AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000644

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000644.t1 MTR_5g028450 69.967 303 60 2 569 841 1 302 2.62e-144 428
MS.gene000644.t1 MTR_5g028510 69.401 317 90 3 107 422 18 328 1.18e-141 422
MS.gene000644.t1 MTR_5g028190 54.366 355 122 5 511 833 185 531 7.49e-119 370
MS.gene000644.t1 MTR_5g028190 47.945 219 86 5 205 421 5 197 4.48e-45 171
MS.gene000644.t1 MTR_5g028930 49.575 353 146 3 518 839 270 621 4.59e-106 340
MS.gene000644.t1 MTR_5g028930 48.084 287 121 10 126 407 1 264 2.89e-60 216
MS.gene000644.t1 MTR_5g028130 49.560 341 131 5 528 833 1 335 2.40e-103 323
MS.gene000644.t1 MTR_5g028830 40.584 308 138 8 366 669 22 288 1.29e-55 194
MS.gene000644.t1 MTR_5g028690 48.077 208 76 3 665 841 36 242 3.80e-53 185
MS.gene000644.t1 MTR_2g438740 34.589 292 159 4 572 834 279 567 1.27e-43 167
MS.gene000644.t1 MTR_3g055310 33.858 254 165 2 527 779 14 265 8.40e-43 159
MS.gene000644.t1 MTR_3g055310 33.858 254 165 2 527 779 77 328 3.60e-42 159
MS.gene000644.t1 MTR_3g055310 33.858 254 165 2 527 779 129 380 1.45e-41 159
MS.gene000644.t1 MTR_5g091220 33.138 341 191 6 527 836 270 604 1.72e-41 161
MS.gene000644.t1 MTR_3g055310 33.858 254 165 2 527 779 157 408 1.78e-41 159
MS.gene000644.t1 MTR_3g055310 33.858 254 165 2 527 779 155 406 2.12e-41 159
MS.gene000644.t1 MTR_3g055310 33.858 254 165 2 527 779 182 433 2.45e-41 159
MS.gene000644.t1 MTR_3g455790 30.183 328 192 4 520 816 258 579 3.48e-41 160
MS.gene000644.t1 MTR_5g028920 54.483 145 62 2 529 672 86 227 9.42e-41 150
MS.gene000644.t1 MTR_2g438440 38.605 215 131 1 567 781 394 607 6.52e-38 152
MS.gene000644.t1 MTR_2g438760 33.725 255 154 2 572 826 273 512 5.92e-37 147
MS.gene000644.t1 MTR_3g452020 32.927 246 162 2 529 773 92 335 1.09e-36 144
MS.gene000644.t1 MTR_2g438700 37.963 216 132 2 567 781 397 611 5.89e-36 145
MS.gene000644.t1 MTR_5g028110 42.188 192 58 4 672 833 4 172 6.72e-36 134
MS.gene000644.t1 MTR_2g438720 37.963 216 132 2 567 781 399 613 7.66e-36 145
MS.gene000644.t1 MTR_3g452020 32.927 246 162 2 529 773 268 511 1.37e-35 144
MS.gene000644.t1 MTR_2g438400 36.667 210 132 1 572 781 258 466 5.59e-35 141
MS.gene000644.t1 MTR_2g438640 33.846 260 156 3 567 826 399 642 1.13e-34 142
MS.gene000644.t1 MTR_6g017215 42.857 189 73 4 670 828 6 189 1.31e-33 129
MS.gene000644.t1 MTR_5g028840 38.725 204 93 3 670 842 2 204 2.73e-33 127
MS.gene000644.t1 MTR_5g028900 57.658 111 35 2 671 781 3 101 2.24e-32 123
MS.gene000644.t1 MTR_2g020450 30.247 324 185 8 552 840 372 689 4.19e-32 134
MS.gene000644.t1 MTR_2g020510 29.553 291 170 4 569 829 390 675 1.06e-30 129
MS.gene000644.t1 MTR_2g438640 31.923 260 135 4 567 826 399 616 3.18e-29 124
MS.gene000644.t1 MTR_3g058570 33.484 221 137 3 568 781 83 300 3.23e-28 118
MS.gene000644.t1 MTR_2g017930 37.387 222 129 4 562 781 352 565 1.91e-26 116
MS.gene000644.t1 MTR_2g020470 29.508 305 159 6 553 826 367 646 3.95e-25 112
MS.gene000644.t1 MTR_1g113820 31.455 213 144 2 567 779 274 484 8.95e-25 110
MS.gene000644.t1 MTR_5g085340 29.911 224 140 3 569 781 443 660 6.46e-24 108
MS.gene000644.t1 MTR_5g085350 29.954 217 147 2 569 781 424 639 1.24e-23 107
MS.gene000644.t1 MTR_5g085350 29.954 217 147 2 569 781 437 652 1.28e-23 107
MS.gene000644.t1 MTR_2g438470 41.353 133 77 1 614 746 366 497 2.95e-23 104
MS.gene000644.t1 MTR_3g050500 29.224 219 146 4 568 780 427 642 2.49e-21 100
MS.gene000644.t1 MTR_1g113760 27.239 268 182 4 560 817 231 495 2.96e-20 95.9
MS.gene000644.t1 MTR_5g085360 24.332 337 189 9 569 842 405 738 1.64e-18 91.3
MS.gene000644.t1 MTR_1g113730 26.638 229 166 2 560 788 37 263 2.08e-18 88.6
MS.gene000644.t1 MTR_3g051060 26.291 213 153 3 570 779 435 646 5.23e-17 86.3
MS.gene000644.t1 MTR_2g438640 33.758 157 88 3 670 826 399 539 9.27e-17 85.1
MS.gene000644.t1 MTR_2g438580 37.805 82 50 1 685 766 3 83 6.46e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000644.t1 AT3G18670 37.931 348 184 6 516 834 295 639 3.06e-65 231
MS.gene000644.t1 AT3G18670 29.767 215 109 6 219 427 88 266 3.69e-17 86.7
MS.gene000644.t1 AT3G18670 38.393 336 175 6 528 834 252 584 4.99e-65 229
MS.gene000644.t1 AT3G18670 28.500 200 101 6 219 412 88 251 2.07e-12 71.2
MS.gene000644.t1 AT5G04730 34.718 337 190 5 527 834 254 589 1.57e-51 191
MS.gene000644.t1 AT5G04700 35.860 343 179 7 526 834 321 656 5.68e-49 184
MS.gene000644.t1 AT5G04690 32.743 339 188 6 527 834 283 612 9.16e-46 174
MS.gene000644.t1 AT5G04690 32.743 339 188 6 527 834 303 632 1.20e-45 174
MS.gene000644.t1 AT5G04690 32.743 339 188 6 527 834 317 646 1.42e-45 174
MS.gene000644.t1 AT5G04680 33.807 352 182 8 526 834 185 528 1.22e-43 167
MS.gene000644.t1 AT5G35810 38.356 219 129 3 569 781 35 253 1.97e-43 161
MS.gene000644.t1 AT5G04680 33.807 352 182 8 526 834 294 637 4.24e-43 167
MS.gene000644.t1 AT5G04680 33.807 352 182 8 526 834 308 651 4.92e-43 167
MS.gene000644.t1 AT5G04680 33.807 352 182 8 526 834 337 680 8.15e-43 167
MS.gene000644.t1 AT3G54065 37.264 212 105 5 656 841 8 217 1.11e-32 127
MS.gene000644.t1 AT5G04700 34.694 147 90 3 527 670 322 465 1.98e-12 70.9

Find 175 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACTTTCATAAATAATT+TGG 0.159583 5.1:+19415654 None:intergenic
CTCTTGAATCTGGGTCATTT+TGG 0.169381 5.1:+19418470 None:intergenic
CATTATCGACATGATCTTAA+AGG 0.186021 5.1:-19411278 MS.gene000644:CDS
TTGTCCATCCTTCATCTTCT+TGG 0.196052 5.1:+19418579 None:intergenic
CAGAACAATTCTTTATTCTT+TGG 0.203069 5.1:+19416454 None:intergenic
GAGTATGATATTGCCGAAAT+TGG 0.213255 5.1:-19414206 MS.gene000644:CDS
ATCTGCAGGGGCAAGGTCTA+TGG 0.241341 5.1:-19418645 MS.gene000644:CDS
GTACTACAATGAACCACTTA+TGG 0.252983 5.1:+19411245 None:intergenic
TGTATCACTGCTGAAATATT+TGG 0.253668 5.1:-19416004 MS.gene000644:intron
AAGAGAGATTCAATGGTTTA+AGG 0.258197 5.1:-19412252 MS.gene000644:intron
CGTTGTTCTAATAATGCTTT+CGG 0.260371 5.1:-19414078 MS.gene000644:CDS
TACCATGCACTTTGCTATTT+CGG 0.263241 5.1:+19416213 None:intergenic
TGATATCGATATTTCTGTTT+TGG 0.276216 5.1:-19413872 MS.gene000644:CDS
GTTGTTCTAATAATGCTTTC+GGG 0.284513 5.1:-19414077 MS.gene000644:intron
TCAACGGCTGAAAGAAAATT+AGG 0.286805 5.1:+19412476 None:intergenic
CAGCAACACATGTTACCTTT+TGG 0.289716 5.1:+19414174 None:intergenic
GCGCTTGTTGCACGTTATAC+TGG 0.297367 5.1:-19416241 MS.gene000644:CDS
CAGTCTGGATAACGCCTAAA+AGG 0.301425 5.1:+19416768 None:intergenic
CAAAACTCGATTCTGTGAAT+TGG 0.302690 5.1:+19416039 None:intergenic
TAAATGATTCCACGCGCTTT+TGG 0.303813 5.1:+19416631 None:intergenic
TCTTGGGTAGCTCTTGAATC+TGG 0.311187 5.1:+19418460 None:intergenic
TTGGACGAAGTTCCCAATTT+CGG 0.318636 5.1:+19414193 None:intergenic
TTCAAGAGCTACCCAAGATT+TGG 0.321701 5.1:-19418455 MS.gene000644:CDS
TTTACGTACCATTACTATCT+AGG 0.330681 5.1:+19413635 None:intergenic
TATATATGACATCGGCTTCC+AGG 0.333476 5.1:-19416381 MS.gene000644:CDS
GTGCATGGTAGAGGAAATAA+AGG 0.338215 5.1:-19416200 MS.gene000644:CDS
AATCATTTAGGCTCATTATA+TGG 0.343162 5.1:-19416617 MS.gene000644:CDS
CCGTGGAGTTGCATATTTGT+TGG 0.349278 5.1:-19413674 MS.gene000644:CDS
GGCACCCGATTGGGATTTCT+AGG 0.351879 5.1:-19415677 MS.gene000644:CDS
TTGCACCTGGCAGCACATTT+AGG 0.356259 5.1:-19412323 MS.gene000644:CDS
ACTAACCTTGCTCCAAATCT+TGG 0.357576 5.1:+19418443 None:intergenic
ATTCTTCTAGCTGCCGCTAA+TGG 0.360168 5.1:-19416127 MS.gene000644:CDS
GACATACCCAAAAGCTTTAC+TGG 0.361682 5.1:+19413849 None:intergenic
ATTTATCAGGTAAGGATTGC+TGG 0.365909 5.1:+19415747 None:intergenic
CAAAGAATAAAGAATTGTTC+TGG 0.367690 5.1:-19416453 MS.gene000644:CDS
TACTACAATGAACCACTTAT+GGG 0.368135 5.1:+19411246 None:intergenic
CATACGAAAATATGCCTCTA+TGG 0.371634 5.1:+19412447 None:intergenic
TTTGTCAAACACTTTGGATT+TGG 0.373648 5.1:+19416063 None:intergenic
TACACACATTATGAAGTTAT+AGG 0.373654 5.1:-19412635 MS.gene000644:CDS
ACTAAGGCCGAGAATTTATC+AGG 0.379921 5.1:+19415734 None:intergenic
CTTTAAGATCATGTCGATAA+TGG 0.388292 5.1:+19411279 None:intergenic
GCAACATCTTATACAATTGT+AGG 0.407639 5.1:-19411593 MS.gene000644:CDS
CTTCAATTATGTCAGGAATC+AGG 0.410277 5.1:-19412676 MS.gene000644:intron
GCTGCAGCCTTTACAGTGCC+AGG 0.415001 5.1:-19411545 MS.gene000644:CDS
TTTCCAAGTGGCACCCGATT+GGG 0.417478 5.1:-19415686 MS.gene000644:CDS
TGATGCGTCATGGAAAATAA+AGG 0.419406 5.1:-19416729 MS.gene000644:CDS
TTTCAAATTCTGAAACAGTC+TGG 0.425070 5.1:+19416753 None:intergenic
TTAGCGGCAGCTAGAAGAAT+AGG 0.427359 5.1:+19416130 None:intergenic
GAACTCGATTATTCCGTGTT+GGG 0.428128 5.1:+19412523 None:intergenic
CTTTCAATGAATATCTCATA+AGG 0.433992 5.1:+19411662 None:intergenic
GGTGGAAACGACGACAAAAC+TGG 0.435847 5.1:-19411524 MS.gene000644:CDS
ATCCTTACCTGATAAATTCT+CGG 0.437868 5.1:-19415741 MS.gene000644:CDS
ATTGGATTTGTCAAACACTT+TGG 0.438556 5.1:+19416057 None:intergenic
TAGTGAAAGATGGAGAGAAA+TGG 0.438852 5.1:-19411628 MS.gene000644:CDS
TCAAACACTTTGGATTTGGT+GGG 0.439060 5.1:+19416067 None:intergenic
ATTATCGACATGATCTTAAA+GGG 0.440142 5.1:-19411277 MS.gene000644:CDS
GATATTACAAGAACGATAAT+CGG 0.441300 5.1:+19411199 None:intergenic
GTCAAACACTTTGGATTTGG+TGG 0.443427 5.1:+19416066 None:intergenic
CTCCGTCCCTACGTTGTTGA+GGG 0.447774 5.1:-19415813 MS.gene000644:CDS
AGTATGATATTGCCGAAATT+GGG 0.448600 5.1:-19414205 MS.gene000644:CDS
CAAACACTTTGGATTTGGTG+GGG 0.451286 5.1:+19416068 None:intergenic
CCCAGAAACGTTGCTAGAAA+AGG 0.455513 5.1:-19416173 MS.gene000644:CDS
GTGATGAGAGGTACGCCTAT+TGG 0.457145 5.1:-19416427 MS.gene000644:CDS
GGTACGCCTATTGGAAATAT+CGG 0.457767 5.1:-19416418 MS.gene000644:CDS
CTTCACGCAGCAGTGATTGC+TGG 0.458933 5.1:-19416349 MS.gene000644:CDS
TGCTGAATCTCGTACTGATC+AGG 0.459882 5.1:-19418605 MS.gene000644:CDS
AGTAGTATGATGAAATCAAC+AGG 0.460104 5.1:-19418745 None:intergenic
GGCCGAGAATTTATCAGGTA+AGG 0.460680 5.1:+19415739 None:intergenic
CAACTCATACATTCTGTCAA+TGG 0.460886 5.1:-19412395 MS.gene000644:CDS
TCATTTGTTTATTGAACGGA+TGG 0.471650 5.1:+19411129 None:intergenic
TACCATTACTATCTAGGTAA+TGG 0.473551 5.1:+19413641 None:intergenic
AAATGCAAAGAGAGATTCAA+TGG 0.476952 5.1:-19412259 MS.gene000644:CDS
ATTCTCGGCCTTAGTTGCAT+TGG 0.477418 5.1:-19415726 MS.gene000644:CDS
AGACATCGTATAGACTCGTT+TGG 0.481995 5.1:-19412356 MS.gene000644:CDS
AAACACTTTGGATTTGGTGG+GGG 0.483841 5.1:+19416069 None:intergenic
CAATTCTTTGTGTCCATTAG+CGG 0.484696 5.1:+19416114 None:intergenic
TTGCATTTGCAGAGCAGCAC+CGG 0.486301 5.1:+19412274 None:intergenic
CTAACCTTGCTCCAAATCTT+GGG 0.486417 5.1:+19418444 None:intergenic
TGTGGAAAATGTGGAATTGT+TGG 0.486891 5.1:-19416323 MS.gene000644:CDS
AAAAGCGCGTGGAATCATTT+AGG 0.488436 5.1:-19416629 MS.gene000644:CDS
GTCGTCATTTGTTTATTGAA+CGG 0.491644 5.1:+19411125 None:intergenic
AATTGGGAACTTCGTCCAAA+AGG 0.492844 5.1:-19414189 MS.gene000644:CDS
TGTTATGCAAAACTATCTGC+AGG 0.494906 5.1:-19418659 MS.gene000644:CDS
TATATTGATAATGATTGCTC+GGG 0.495481 5.1:+19415842 None:intergenic
AGTGAAAGATGGAGAGAAAT+GGG 0.498200 5.1:-19411627 MS.gene000644:CDS
ATATATTGATAATGATTGCT+CGG 0.502639 5.1:+19415841 None:intergenic
CCTTTCTGCCTATGCCACAT+TGG 0.504501 5.1:+19414111 None:intergenic
AATCCCAATCGGGTGCCACT+TGG 0.507477 5.1:+19415683 None:intergenic
CTAAAGACAACTATGAAAGA+TGG 0.509180 5.1:-19418687 MS.gene000644:CDS
TCAACAGGTGTTGCCTCTGA+TGG 0.509585 5.1:-19418730 MS.gene000644:CDS
TGAACTCGATTATTCCGTGT+TGG 0.510589 5.1:+19412522 None:intergenic
TGCTGGAAATGTGGAAAATG+TGG 0.512870 5.1:-19416332 MS.gene000644:CDS
CTTGGGTAGCTCTTGAATCT+GGG 0.512944 5.1:+19418461 None:intergenic
ATACTCCCAAGTGCCATGAT+TGG 0.513042 5.1:+19411223 None:intergenic
CATCTTTCACTAGCTCCTCA+TGG 0.514505 5.1:+19411639 None:intergenic
TTCCATTACCTAGATAGTAA+TGG 0.516269 5.1:-19413643 MS.gene000644:intron
TATGGCAAAGATTTAACAGC+CGG 0.518073 5.1:-19416599 MS.gene000644:CDS
TATCGTTCTTGTAATATCAC+AGG 0.519358 5.1:-19411194 MS.gene000644:CDS
TAGCGGCAGCTAGAAGAATA+GGG 0.520879 5.1:+19416131 None:intergenic
TTTGGCCTAGAAATCCCAAT+CGG 0.521150 5.1:+19415672 None:intergenic
AGTTACTTCTGCCGAAGTGT+TGG 0.521693 5.1:-19412605 MS.gene000644:CDS
TCTGCAGGGGCAAGGTCTAT+GGG 0.522236 5.1:-19418644 MS.gene000644:CDS
AATAGCAAAGTGCATGGTAG+AGG 0.523953 5.1:-19416209 MS.gene000644:CDS
TATGTTGTCATGTAAGAAGA+TGG 0.525735 5.1:+19411496 None:intergenic
TCTGATCGTCATAGCAGATC+CGG 0.526463 5.1:-19412293 MS.gene000644:CDS
TCTCCGTCCCTACGTTGTTG+AGG 0.527493 5.1:-19415814 MS.gene000644:CDS
TCAGGTAAGGATTGCTGGAG+AGG 0.527967 5.1:+19415752 None:intergenic
TGGCAATAATTTGTTGCACC+TGG 0.529393 5.1:-19412336 MS.gene000644:CDS
ATTCTTTATTCTTTGGAGAG+TGG 0.532153 5.1:+19416461 None:intergenic
GCCTCTATGGCAGCTATCAA+CGG 0.538256 5.1:+19412460 None:intergenic
CATATTCACTGCATTTCTCA+TGG 0.544524 5.1:-19411477 MS.gene000644:CDS
TGGAATTGTTGGTGAAAGTA+GGG 0.547426 5.1:-19416312 MS.gene000644:CDS
GTTTCCACCTGGCACTGTAA+AGG 0.547912 5.1:+19411538 None:intergenic
TCCATACCGATATTTCCAAT+AGG 0.548152 5.1:+19416412 None:intergenic
CATGAGGAGCTAGTGAAAGA+TGG 0.552759 5.1:-19411638 MS.gene000644:CDS
CTGCTATGACGATCAGATGA+AGG 0.554930 5.1:+19412299 None:intergenic
GTTCATTGTAGTACCAATCA+TGG 0.555376 5.1:-19411236 MS.gene000644:CDS
GTGCCACTTGGAAAAGATGT+AGG 0.556314 5.1:+19415695 None:intergenic
CGAGTTCATAAATGCCATGA+GGG 0.557678 5.1:-19412507 MS.gene000644:CDS
TGCGTGAAGTAGCGAAGACC+TGG 0.560154 5.1:+19416363 None:intergenic
GCCTATTGGAAATATCGGTA+TGG 0.560203 5.1:-19416413 MS.gene000644:CDS
CCAATGTGGCATAGGCAGAA+AGG 0.561188 5.1:-19414111 MS.gene000644:CDS
CATTTGTTTATTGAACGGAT+GGG 0.564108 5.1:+19411130 None:intergenic
GCTACCCAAGATTTGGAGCA+AGG 0.566888 5.1:-19418448 MS.gene000644:intron
TAATAGTTCAAAGCTCAAAG+AGG 0.573010 5.1:-19412576 MS.gene000644:CDS
TCCGCGGTCCAATGTGGCAT+AGG 0.573491 5.1:-19414119 MS.gene000644:CDS
AAGGAAGCCGAAAATGATGA+TGG 0.573881 5.1:-19411692 MS.gene000644:CDS
TTGCAGATTTGAGCGTGGAA+AGG 0.576225 5.1:-19414241 MS.gene000644:intron
CTACTTTGTAAGATATTCCG+TGG 0.578446 5.1:-19413691 MS.gene000644:CDS
AGGGATATGAAACCCATAAG+TGG 0.578644 5.1:-19411258 MS.gene000644:CDS
CAAAACTATCTGCAGGGGCA+AGG 0.578679 5.1:-19418652 MS.gene000644:CDS
ACTTTGTTCATGATCTGCAC+CGG 0.579681 5.1:+19416580 None:intergenic
GAATAAAGAATTGTTCTGGA+AGG 0.580301 5.1:-19416449 MS.gene000644:CDS
GTGGAATTGTTGGTGAAAGT+AGG 0.581941 5.1:-19416313 MS.gene000644:CDS
GTAGTACCAATCATGGCACT+TGG 0.582602 5.1:-19411229 MS.gene000644:CDS
TGCATGGTAGAGGAAATAAA+GGG 0.582858 5.1:-19416199 MS.gene000644:CDS
TCAGGCTTCCAAGAAGATGA+AGG 0.586986 5.1:-19418587 MS.gene000644:CDS
ATGTTGTCATGTAAGAAGAT+GGG 0.590707 5.1:+19411497 None:intergenic
ATGAAATCTTCCGCTCAACA+TGG 0.593061 5.1:-19411159 MS.gene000644:CDS
ATGATTCCCTCAACAACGTA+GGG 0.595992 5.1:+19415807 None:intergenic
AATTTGAAACTGATGCGTCA+TGG 0.599011 5.1:-19416739 MS.gene000644:CDS
ATGTGGCTTTGAGATTACTC+AGG 0.601489 5.1:-19415880 MS.gene000644:CDS
GCTTCCAAGAAGATGAAGGA+TGG 0.601738 5.1:-19418583 MS.gene000644:CDS
TTGGCCTAGAAATCCCAATC+GGG 0.602780 5.1:+19415673 None:intergenic
CATATCATTCAACTATCATG+TGG 0.604069 5.1:-19416692 MS.gene000644:CDS
ACCACAAAAGATTTCTTCCG+CGG 0.610501 5.1:-19414135 MS.gene000644:CDS
TACCGAAATAGCAAAGTGCA+TGG 0.611483 5.1:-19416215 MS.gene000644:CDS
CTCGAATGTATATATGACAT+CGG 0.616856 5.1:-19416389 MS.gene000644:CDS
ACATTCTGTCAATGGACGGA+AGG 0.617474 5.1:-19412387 MS.gene000644:CDS
GCCGTTGATAGCTGCCATAG+AGG 0.619118 5.1:-19412461 MS.gene000644:CDS
ATTAGGATTAGCATCCCTCA+TGG 0.622934 5.1:+19412493 None:intergenic
TTAAAACAGCCAAAAGCGCG+TGG 0.623364 5.1:-19416640 MS.gene000644:CDS
TAGTACCAATCATGGCACTT+GGG 0.623895 5.1:-19411228 MS.gene000644:CDS
GTTATGCAAAACTATCTGCA+GGG 0.624004 5.1:-19418658 MS.gene000644:CDS
TTCCCTCAACAACGTAGGGA+CGG 0.626973 5.1:+19415811 None:intergenic
AAGGACCTAAATGTGCTGCC+AGG 0.631007 5.1:+19412318 None:intergenic
TCATACATTCTGTCAATGGA+CGG 0.635424 5.1:-19412391 MS.gene000644:CDS
GCCTATGCCACATTGGACCG+CGG 0.638415 5.1:+19414118 None:intergenic
TTGATGAACTATTCCATCAG+AGG 0.645567 5.1:+19418717 None:intergenic
TTATGCAAAACTATCTGCAG+GGG 0.646152 5.1:-19418657 MS.gene000644:CDS
AATGATTCCCTCAACAACGT+AGG 0.651538 5.1:+19415806 None:intergenic
CCAACAAATATGCAACTCCA+CGG 0.652186 5.1:+19413674 None:intergenic
AGCAGTGATTGCTGGAAATG+TGG 0.655602 5.1:-19416341 MS.gene000644:CDS
TATACCAATGAAAATCAAGA+CGG 0.658060 5.1:+19411421 None:intergenic
ATTTCTTCCGCGGTCCAATG+TGG 0.663733 5.1:-19414125 MS.gene000644:CDS
ATATGCATGCAAGACGAACA+CGG 0.664176 5.1:-19416277 MS.gene000644:CDS
ATGTTGCATGCATCCCAACA+CGG 0.665878 5.1:-19412536 MS.gene000644:CDS
TTGCATTGCAGATTTGAGCG+TGG 0.668467 5.1:-19414246 MS.gene000644:intron
TTGTTCTGGAAGGTGATGAG+AGG 0.672631 5.1:-19416439 MS.gene000644:CDS
AAAGGCTGCAGCAAACATGA+TGG 0.673254 5.1:+19411556 None:intergenic
ATGCAACATTGCTTCATACG+CGG 0.681582 5.1:+19412550 None:intergenic
TCGAGTTCATAAATGCCATG+AGG 0.690060 5.1:-19412508 MS.gene000644:CDS
GCAGCCTTTACAGTGCCAGG+TGG 0.698792 5.1:-19411542 MS.gene000644:CDS
GGCTGCAGCAAACATGATGG+TGG 0.726270 5.1:+19411559 None:intergenic
GATATTCATTGAAAGCCATG+AGG 0.729208 5.1:-19411654 MS.gene000644:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.1 gene 19411129 19418761 19411129 ID=MS.gene000644
chr5.1 mRNA 19411129 19418761 19411129 ID=MS.gene000644.t1;Parent=MS.gene000644
chr5.1 exon 19418449 19418761 19418449 ID=MS.gene000644.t1.exon1;Parent=MS.gene000644.t1
chr5.1 CDS 19418449 19418761 19418449 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19416582 19416797 19416582 ID=MS.gene000644.t1.exon2;Parent=MS.gene000644.t1
chr5.1 CDS 19416582 19416797 19416582 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19416005 19416496 19416005 ID=MS.gene000644.t1.exon3;Parent=MS.gene000644.t1
chr5.1 CDS 19416005 19416496 19416005 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19415660 19415902 19415660 ID=MS.gene000644.t1.exon4;Parent=MS.gene000644.t1
chr5.1 CDS 19415660 19415902 19415660 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19414078 19414257 19414078 ID=MS.gene000644.t1.exon5;Parent=MS.gene000644.t1
chr5.1 CDS 19414078 19414257 19414078 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19413856 19413915 19413856 ID=MS.gene000644.t1.exon6;Parent=MS.gene000644.t1
chr5.1 CDS 19413856 19413915 19413856 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19413644 19413718 19413644 ID=MS.gene000644.t1.exon7;Parent=MS.gene000644.t1
chr5.1 CDS 19413644 19413718 19413644 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19412253 19412683 19412253 ID=MS.gene000644.t1.exon8;Parent=MS.gene000644.t1
chr5.1 CDS 19412253 19412683 19412253 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19411411 19411744 19411411 ID=MS.gene000644.t1.exon9;Parent=MS.gene000644.t1
chr5.1 CDS 19411411 19411744 19411411 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
chr5.1 exon 19411129 19411316 19411129 ID=MS.gene000644.t1.exon10;Parent=MS.gene000644.t1
chr5.1 CDS 19411129 19411316 19411129 ID=cds.MS.gene000644.t1;Parent=MS.gene000644.t1
Gene Sequence

>MS.gene000644

ATGATGAAATCAACAGGTGTTGCCTCTGATGGAATAGTTCATCAAGTACTCACTAAAGACAACTATGAAAGATGGAAAATTGTTATGCAAAACTATCTGCAGGGGCAAGGTCTATGGGACGTTGTCATACAAAATGCTGAATCTCGTACTGATCAGGCTTCCAAGAAGATGAAGGATGGACAACAAGCTTTACACTTCTTTCAACTATCATCATGTAAACGAGATTTTCTTGAAACAGTCAAAAAATCAGGCTGGAATAACTTCACCAACCAAAATGACCCAGATTCAAGAGCTACCCAAGATTTGGAGCAAGGTTAGTATCTGTATACTCATATGTCATATCCATATTTTTTTAGTACAACTCATCACTGTGATACTATTACTCGCTTTCTCTATTGTTTTATTAAGGCTTAATTAATGATTTGGTCCCTTAAGTTATTTGTTGGTTACATTTTGGTCCCATTAATTATTAACGTTACTATTTGGTCCCTTAAGTTAGTATCTGTTAGCCAAATAAATCCTCACCGTCAGTCTTTTGCCAAAACGTTAAATAGGCACATGTGAACGGTGACGTGACAACTTGCAATGTGTATCCACCTTAAACATTGGCTTAACGGTTTTGACGGTGGGAATCTATTTGGTTGATGGAAGATGACTTAATGAGACCAAATTGTAACATTAATAACTTATGCGACTAAAATGTAACCAACAAATAACTTAAGGGACCACATCATTAATTAAGTCTTTTATTAATCATGGCGACATCTAAATTTACTTGTAAATATTTTTGGTGGTGGCCGGGGTATGAGCCTCGGACCTTACATATATTTGATATATTTGATTGTAAATAACATAATCTATTTTTGATTGTAAATATTTGATTGTAAATATTTGATTGTAAACTCACGAAGACTTTATTTGTATATATTTGATTGTAAATAACATAATCTATTTTTTTCGATAATGGTATATATCTAGGCACACCCAATTTTTCTACATCCATTCAACAATTCATTTTCTCTATATCTCTCTTCATAGCACATCATGAATCTATCACATTCACTCTCTTTTATCTTTATCTCTTTCAAATATACATTTGGAGTGTCTAAGTAACATTTTTTTCTTCTTAAAATCAAATACTCTTCATCTATACTATATACAAAGAAAATAAGCTTAAAATACATGAGTTTGAGCTAACTTTTTCATTACCTATTTTACCCTTCATTTAAACTATTTTATTTACTATTTTATCCTTTAATAATATTATTTTAAATTTTAATTAAAAAATTTAAATCCCATGAAAATAGGAATTATCCCACTACAATAAGAACAATATGTAAACAAATTATCTTGTATTAATATTATATTGTTGGTGGTATTAAAAAAGATAAGAATTTATATTATATTTGTTAAATTACTGTTATTATTGAAAAAGATAAAGATGATTTTTTGGGATTGTTACAATTTAAAAGTCACAAATATTTGTACTTATAATTTGAATCAAAATTAAAATTTCATATAAAATACCGTTCATAATTTACATGGGTCAGGGCAATAAAAAGAGCGGAAAGACGGACACATGAGTGCCGTCTTTTCGCTAGGAATACATAAATCAATTTAATATAAATAAATTTGATAGAAACTTAAACAAAACAACAAGGACGACTTACTTTTTTTTTTAATCAAACCAAGGACTTTAAATTTTAACTAAGGACTTGCTTAAACAAGCCGCAACTTTTAAACTTATGAGTCATTATGTCTGACTCAAATATTAATAGGACCAACAACTTGTTATATTGAAGTTGTGTTTAATTAACTTTATATTGCACATCTGGTCCTCGTTGTTACATTTTAAGTGTTTAGCTGTAAGAATATTTTATGTGGAATTACAAATTCAAACATTAATGTATATGTTTATGTTTCCCACTAAATATTTTTGATATTTCCCTCTATGAATATGTTAGGGCAAGGCCTTTTAGGCGTTATCCAGACTGTTTCAGAATTTGAAACTGATGCGTCATGGAAAATAAAGGATGTGAAAGCTTTGCATATCATTCAACTATCATGTGGACGAGAGATTGCTGATGAGATTAGTCATTTTAAAACAGCCAAAAGCGCGTGGAATCATTTAGGCTCATTATATGGCAAAGATTTAACAGCCGGTGCAGATCATGAACAAAGTACCATTAACTGATTTAAAAGTTTCATAAACTGATTTTTATGACAATTAATGTGCCTCATCAAACTCTTGAATGTTAATGGCAGATGTGGATGATAGCCACTCTCCAAAGAATAAAGAATTGTTCTGGAAGGTGATGAGAGGTACGCCTATTGGAAATATCGGTATGGACTCGAATGTATATATGACATCGGCTTCCAGGTCTTCGCTACTTCACGCAGCAGTGATTGCTGGAAATGTGGAAAATGTGGAATTGTTGGTGAAAGTAGGGAAAGACACGTTAATATGCATGCAAGACGAACACGGTGACACAGCTCTTGCGCTTGTTGCACGTTATACTGGAAATACCGAAATAGCAAAGTGCATGGTAGAGGAAATAAAGGGACTCCCAGAAACGTTGCTAGAAAAGGAGAACAATGACAATGTAATCCCTATTCTTCTAGCTGCCGCTAATGGACACAAAGAATTGACTTCCTATCTTTACTCCAAAACCCCCACCAAATCCAAAGTGTTTGACAAATCCAATTCACAGAATCGAGTTTTGCTTCTTTCACTATGTATCACTGCTGAAATATTTGGTAAGTAAATTTATAGTTTTTGTATGTTGTTAATTTCTTTTTGGTATTTTGATGTAATACTTGTTTTATTCAAATGTGTGGTTTGATTTGATCCATGATCAGATGTGGCTTTGAGATTACTCAGGCGTTTCAAAGATCTGCCCGAGCAATCATTATCAATATATAACTTCTCCGTCCCTACGTTGTTGAGGGAATCATTATCACTACCTAGCAAAATATCAGAATCAGATCCTCTCCAGCAATCCTTACCTGATAAATTCTCGGCCTTAGTTGCATTGGCAAAAATGCCTACATCTTTTCCAAGTGGCACCCGATTGGGATTTCTAGGCCAAATTATTTATGAAAGTAAGTTTTCACACTATTCTTTCTTTATTGGTAATAGTGATAACCAGGCAACATTCCAATACCCTGAAATACATATTGTAAAAGAAAAATAGTTTAAATCAAGTTAGATGGTTAATACATATAACGGCAACAACAAAACCCAATAGATACTATTCAGATTAAACTCAAGATAATATGTCATGTAATTGATTTTGATTGTGTTGATAAACTTATCTCTTTGAGTTAAAACATGTGGGTTCAATGTGGCTGCAATAGAAAAATACATAAAACCTAAGTTAGTAATATGATAATAGCGAATTGATGATGGAGATTATGAAAGATGATAGAATAATAAGATACATGTCAAATATTGTAAAGCATGAGCATCCTCAACACTGAGATTTTCCATGAGCATGTGAGATTGAGGTTCAACTGGTATGCAATGACGTCGCATAAAAATAATGAGCATCAATTAATATAATAAGAAGCGGGTTGTGAGTTTTTACGGTTTTGGTGATGTGGTGCAATTACTGAGATTATGGGTGGGAATTAGGCTTAAGAGGGGATTAGGGTTGAGCATAATTTAAGAGACATTTATAATAATAGAATAGATACATAAATATAATATGATACAATGGTTATGGTAATTTGTACATTTATCGCGTATTAATAAGTTATTTAAAAAGGATATGGAACAAATATAAGAAAAAGGTAACCGAGTGATTCAACTCAAGTGATTAATGAACTTCAAATGATGAATCGTTCGGGAGAACAAGTTGGATTTTTGGATGAAACAATTAAAATCTCACAACCAAACTACAGATTACTAGGGGGACTCTTTTTCCTGAGATCTAAAGGATTAACAAAAATAAAAAAAAAAAAAAGATAAGTAAAGGGATATGTTAGTATATCCATGATGATGATCATTTAAAATTTACCAGTTCTTTATCAACATTAGTGATTAACTTTATATTATTCCTTCCGGTTCTTTTTATAAAAGATAAATGTGTCAACAAAAGTTGATGTATTTAGTTTATAATATTGACTAAATACATCAATTTTCGTTGATCCATTTTTCTCTTATAAAAAAGTACCGAAAGGAGAGTATGTAGATATTTATCCATATAATGATAGTTAAATATTAGATCATAAAAGAAAGAAAGAAACAATGTAATAAAATATTTGAATAGAGGAGGATTGCTATATGCTGCGAAGGAAATGATGCACGACCTGGATACGACAAAATGTGGGCAATGCTTTCACACTTTTAAAATAAATAAAGTAAAACACCACTTAAAGCAAAACTAAAGGGCTGGTGTGCATTCGTCGTTATTCGTCAACTCCTCTCTCGCACCATTTATCTTTCTCCATTTGAATAAACCTCTTTTAAAAAGAAAATATTTGAACATAATTTGCATTGCAGATTTGAGCGTGGAAAGGAGTTTCAACATTGAGTATGATATTGCCGAAATTGGGAACTTCGTCCAAAAGGTAACATGTGTTGCTGATGCTGCTAATGAAAGACCACAAAAGATTTCTTCCGCGGTCCAATGTGGCATAGGCAGAAAGGTTTTGAATTTCGTTGTTCTAATAATGCTTTCGGGTACGCTATGTACTTTCTCTTTAACTATTTCAGTCTTCCAACATCACATATTAATAATAAAGACCTATGCCTTTTAACCGGTACTTTTATGACACAAGTTAGCAAAACCTGTTTTTCATTACCCACATGAATATATATAAATGGCTCAAAGCTTTTATTTAGGTTGGCGCTTCTGCAAACTGTTGATATCGATATTTCTGTTTTGGCCAGTAAAGCTTTTGGGTATGTCGTGTACTGTCTCAGTTTGCAATATCATGCTTTAATCATTTTTTATTTCTTCTCTGATATTTTTTCTTTTTATTATTTTTTTTAAGGATTTCTTTTTACTATTTCTACACTGATTTTGTTGATTTGTTCAGGTCAACTACTTTGTAAGATATTCCGTGGAGTTGCATATTTGTTGGTTCAAGTTTTCCATTACCTAGATAGTAATGGTACGTAAATTCAAATGCAAAAGTTTGATTCATTTCAATAATCATCCAGTAAAAAAAACTCATTAATTATTCCCTCACATTTCAAATATGTGATGAATTAGGGGAGCGGTTAAAAAGTACGACTGATTTTTATACGAAAATTCACGAAGTGCAAATTGATATAAAAAAAAATGTGTAATTTCCCCTCGTTTCTTTGAAAAAAGAACTAGATTAAACTAAAAAATAAAAAATAGAACAAACTTGACATAAAAGCTTTCTCATGCCAATAAGAGTGAAGTCGGCAAAATTTGGAACAAGGGAGTTTAAAATCAATTTCATTATCTTTTGCAAGCTGCTTTGAAAAATCAATTCAAAAAACGTGTTAGCATGGAAGATGCCGACTCATAAATCACATCATGACGAATTTAAAGTTATCTTTTACTCTCAAATTAAAAAGTTGTTTAAATAATGGAAAAAAATTACTTTGATTCATGAAATTTTGTATATACGATCAAATATTGGTTTCACTAATTAGAAAACTTCAATGTTCCAACCTGCAAAACTGTCTGCAACTCATGCGGAAACCTATATCAGTAGGAAAACATGTAACATCAGCTATCACACGAGTCAATTCGCTTTGCACCAATACTAATAACAATTGATATACTAATTTGCAATAGACACGAATACAATTATACACTATAAACCATACACCAGTCAACAAATCGATCGAACCGCAAAATATTGAACCATATTTAAAACATAATCCCTCCTACAATATAATTACTAATATAGCTGGAACGTCAACAGATCATAGTGATGTCTTAGCATTTAAAATATTGGTTAAAAAAATCATTATGATTGATAGTTTAGAAAAAATCTTGATATTGATGTTGCATGTAAAGCTTTCCTTATATGCTTAATTCGAATTGATTTTTTTTAACAACTCCAAGACTGATTAGGTCCCTTCAATTATGTCAGGAATCAGGAAGATATATGATCAGAAATACACACATTATGAAGTTATAGGAATATTGAGTTACTTCTGCCGAAGTGTTGGAAAATTTAATAGTTCAAAGCTCAAAGAGGCTTCCGCGTATGAAGCAATGTTGCATGCATCCCAACACGGAATAATCGAGTTCATAAATGCCATGAGGGATGCTAATCCTAATTTTCTTTCAGCCGTTGATAGCTGCCATAGAGGCATATTTTCGTATGCAATCTTGCATCGCAAACAAAATGTGTTTCAACTCATACATTCTGTCAATGGACGGAAGGAGATACTTAGACATCGTATAGACTCGTTTGGCAATAATTTGTTGCACCTGGCAGCACATTTAGGTCCTTCATCTGATCGTCATAGCAGATCCGGTGCTGCTCTGCAAATGCAAAGAGAGATTCAATGGTTTAAGGTCACCCATCTAAATTAATTGTCTTAAATATACAATTGATTGAAGATACATATTCCATATTTTAATTCAATATTAGAAGTGCAAAATAATCAACGTATACTAGCTTTGTTGGCCATTTTACAAAACATGGTATAGGAAAATCCTTTTAGATTACATGTTATAAATACATATTAAGCATTTCCTAATAAATGTTGTTGAAACACCATATGTTCAGTACTCTAAAATTACACTCCAACAGTATTAGGTACGAAACACCCCTTCTGTTACGAAATATAAGTAAAAATGAGTTAGTTTTTTTGGACCAAATACATCAAACTTTCGTTAACCAACTTTTGCTTATATTTCGTTACAGATGTTGTTACAAAGCCTCCAAATGAACCAAGATTCATGTGTTTCAACATTGTGATTTTAAAGAAGCTTCAAAACCACATAATTCCACCAATCTATTTAATTTACAGTATATGCATGTCTCTTGCTTCTTTATAAGTTGTTTATGATACCACTGCAGGCGGTTGAGAAAGTTTTACATCCCAAGTTTAAGGAAGCCGAAAATGATGATGGTAAGAAGCCTTATGAGATATTCATTGAAAGCCATGAGGAGCTAGTGAAAGATGGAGAGAAATGGGCAAAAGACACAGCAACATCTTATACAATTGTAGGTACTCTCATCACCACCATCATGTTTGCTGCAGCCTTTACAGTGCCAGGTGGAAACGACGACAAAACTGGTTTGCCCATCTTCTTACATGACAACATATTCACTGCATTTCTCATGGCAGACGCAGTATCTCTCTTCGCATCTGCGACTTCCGTCTTGATTTTCATTGGTATACTCACATCGCGTACGTTATGCTGAGACAGACTTTCTCAAGAGCTTACCTTGGAAGTTATTGTTTGGTCTTTTGCTCCTTTTTCTCTCTGTTTGTTCTATGATAGTTGCATTTAGCGCTGCCATTATCGACATGATCTTAAAGGGATATGAAACCCATAAGTGGTTCATTGTAGTACCAATCATGGCACTTGGGAGTATTCCGATTATCGTTCTTGTAATATCACAGGTGAGCTTCATGTATGAAATCTTCCGCTCAACATGGAAAAATCCCATCCGTTCAATAAACAAATGA
Protein sequence

>MS.gene000644.t1

MMKSTGVASDGIVHQVLTKDNYERWKIVMQNYLQGQGLWDVVIQNAESRTDQASKKMKDGQQALHFFQLSSCKRDFLETVKKSGWNNFTNQNDPDSRATQDLEQGQGLLGVIQTVSEFETDASWKIKDVKALHIIQLSCGREIADEISHFKTAKSAWNHLGSLYGKDLTAGADHEQNVDDSHSPKNKELFWKVMRGTPIGNIGMDSNVYMTSASRSSLLHAAVIAGNVENVELLVKVGKDTLICMQDEHGDTALALVARYTGNTEIAKCMVEEIKGLPETLLEKENNDNVIPILLAAANGHKELTSYLYSKTPTKSKVFDKSNSQNRVLLLSLCITAEIFDVALRLLRRFKDLPEQSLSIYNFSVPTLLRESLSLPSKISESDPLQQSLPDKFSALVALAKMPTSFPSGTRLGFLGQIIYENLSVERSFNIEYDIAEIGNFVQKVTCVADAANERPQKISSAVQCGIGRKVLNFVVLIMLSGWRFCKLLISIFLFWPVKLLGQLLCKIFRGVAYLLVQVFHYLDSNGIRKIYDQKYTHYEVIGILSYFCRSVGKFNSSKLKEASAYEAMLHASQHGIIEFINAMRDANPNFLSAVDSCHRGIFSYAILHRKQNVFQLIHSVNGRKEILRHRIDSFGNNLLHLAAHLGPSSDRHSRSGAALQMQREIQWFKAVEKVLHPKFKEAENDDGKKPYEIFIESHEELVKDGEKWAKDTATSYTIVGTLITTIMFAAAFTVPGGNDDKTGLPIFLHDNIFTAFLMADAVSLFASATSVLIFIGILTSLAFSAAIIDMILKGYETHKWFIVVPIMALGSIPIIVLVISQVSFMYEIFRSTWKNPIRSINK