Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001663.t1 | XP_003597416.1 | 100 | 69 | 0 | 0 | 1 | 69 | 16 | 84 | 2.00E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001663.t1 | Q9ZW33 | 73.8 | 65 | 17 | 0 | 2 | 66 | 15 | 79 | 6.7e-25 | 114.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001663.t1 | G7IRZ4 | 100.0 | 69 | 0 | 0 | 1 | 69 | 16 | 84 | 1.5e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene001663 | MS.gene41171 | PPI |
| MS.gene001663 | MS.gene071173 | PPI |
| MS.gene004041 | MS.gene001663 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001663.t1 | MTR_2g097870 | 100.000 | 69 | 0 | 0 | 1 | 69 | 16 | 84 | 7.41e-48 | 146 |
| MS.gene001663.t1 | MTR_2g097870 | 100.000 | 69 | 0 | 0 | 1 | 69 | 14 | 82 | 1.25e-47 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001663.t1 | AT2G29530 | 73.846 | 65 | 17 | 0 | 2 | 66 | 15 | 79 | 5.52e-35 | 114 |
| MS.gene001663.t1 | AT2G29530 | 73.846 | 65 | 17 | 0 | 2 | 66 | 15 | 79 | 5.52e-35 | 114 |
| MS.gene001663.t1 | AT2G29530 | 52.809 | 89 | 18 | 1 | 2 | 66 | 15 | 103 | 1.18e-28 | 98.6 |
Find 21 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTAATTTGAACAGATATT+TGG | 0.172661 | 2.3:-3652573 | None:intergenic |
| ACATCTTACATGCCAGTATT+TGG | 0.206917 | 2.3:+3651520 | None:intergenic |
| CAAGGAGTCCGAGCTAAATA+TGG | 0.278151 | 2.3:-3651573 | MS.gene001663:CDS |
| AATCTAATTGGTCAGCTTCT+TGG | 0.297313 | 2.3:-3650972 | MS.gene001663:CDS |
| ACCGCTGTGTTTCCAAATAC+TGG | 0.321334 | 2.3:-3651532 | MS.gene001663:CDS |
| TGTCAGGTGACTAATCTAAT+TGG | 0.348592 | 2.3:-3650984 | MS.gene001663:intron |
| ATTGGTCAGCTTCTTGGTAC+TGG | 0.406086 | 2.3:-3650966 | MS.gene001663:CDS |
| TGAAAAGGAGATGGAGTATA+GGG | 0.409805 | 2.3:-3652544 | MS.gene001663:CDS |
| CTGAAAAGGAGATGGAGTAT+AGG | 0.420828 | 2.3:-3652545 | MS.gene001663:CDS |
| GATATTTGGAATGGCTGAAA+AGG | 0.432718 | 2.3:-3652559 | MS.gene001663:CDS |
| CACACTTATTGAAGCAGGAC+TGG | 0.454382 | 2.3:+3651701 | None:intergenic |
| GTCAACACACTTATTGAAGC+AGG | 0.497289 | 2.3:+3651696 | None:intergenic |
| GTCAGCTTCTTGGTACTGGC+GGG | 0.500273 | 2.3:-3650962 | MS.gene001663:CDS |
| ACTTTATCTTCTCAGGTACA+AGG | 0.510118 | 2.3:-3651591 | MS.gene001663:intron |
| GGAAACACAGCGGTCAATGC+AGG | 0.526463 | 2.3:+3651541 | None:intergenic |
| ATTTGAACAGATATTTGGAA+TGG | 0.527257 | 2.3:-3652568 | None:intergenic |
| AAGGAGTCCGAGCTAAATAT+GGG | 0.527587 | 2.3:-3651572 | MS.gene001663:CDS |
| TGGAATGGCTGAAAAGGAGA+TGG | 0.540959 | 2.3:-3652553 | MS.gene001663:CDS |
| GGTCAGCTTCTTGGTACTGG+CGG | 0.562832 | 2.3:-3650963 | MS.gene001663:CDS |
| ACACTTATTGAAGCAGGACT+GGG | 0.620696 | 2.3:+3651702 | None:intergenic |
| GCCAGTATTTGGAAACACAG+CGG | 0.771787 | 2.3:+3651531 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTGATAAAAAAGTAAAAA+AGG | + | chr2.3:3651050-3651069 | None:intergenic | 15.0% |
| !! | AATTATATTTATGCAGTTTA+GGG | - | chr2.3:3651546-3651565 | MS.gene001663:CDS | 15.0% |
| !! | GTTAAAAAAATATGCTAAAA+TGG | + | chr2.3:3652370-3652389 | None:intergenic | 15.0% |
| !! | TAATTATATTTATGCAGTTT+AGG | - | chr2.3:3651545-3651564 | MS.gene001663:CDS | 15.0% |
| !! | TGTTGGCATAATTATTAATT+TGG | - | chr2.3:3651753-3651772 | MS.gene001663:intron | 20.0% |
| !! | TTTCTTCAACTTAATTTCAT+AGG | + | chr2.3:3651269-3651288 | None:intergenic | 20.0% |
| !!! | TTTTTTGCAAATCCAATATT+TGG | - | chr2.3:3652066-3652085 | MS.gene001663:intron | 20.0% |
| ! | AAGAAGTTATGATCTGAATA+TGG | - | chr2.3:3652241-3652260 | MS.gene001663:intron | 25.0% |
| ! | AATAAGTTGTTTATCCAAAC+AGG | + | chr2.3:3651102-3651121 | None:intergenic | 25.0% |
| ! | AGAAACAAACAACCAAATAT+TGG | + | chr2.3:3652081-3652100 | None:intergenic | 25.0% |
| ! | ATAGTTTCTCAAATGTCTAA+AGG | - | chr2.3:3652110-3652129 | MS.gene001663:intron | 25.0% |
| ! | CTGAATGGTATAAACAAAAA+GGG | + | chr2.3:3651784-3651803 | None:intergenic | 25.0% |
| ! | GACAGATTTCATATACTTTA+GGG | - | chr2.3:3651520-3651539 | MS.gene001663:intron | 25.0% |
| ! | TAGTTTAGAGATTGAATTGA+TGG | - | chr2.3:3651590-3651609 | MS.gene001663:intron | 25.0% |
| ! | TGAATGGTATAAACAAAAAG+GGG | + | chr2.3:3651783-3651802 | None:intergenic | 25.0% |
| ! | TGACAGATTTCATATACTTT+AGG | - | chr2.3:3651519-3651538 | MS.gene001663:intron | 25.0% |
| ! | TTTGCAACTAGTTATAATGA+AGG | - | chr2.3:3651725-3651744 | MS.gene001663:intron | 25.0% |
| !! | TCTGAATATGGTTTTTACTT+TGG | - | chr2.3:3652253-3652272 | MS.gene001663:intron | 25.0% |
| !!! | GGATTTTCATATATTTTGAC+AGG | - | chr2.3:3651472-3651491 | MS.gene001663:intron | 25.0% |
| !!! | TCTAAAGGTACAATTTTAGT+TGG | - | chr2.3:3652125-3652144 | MS.gene001663:intron | 25.0% |
| !!! | TTATAATGAAGGTTTGTTGT+TGG | - | chr2.3:3651736-3651755 | MS.gene001663:intron | 25.0% |
| AAAACACCAGTTAGACAATA+AGG | + | chr2.3:3652500-3652519 | None:intergenic | 30.0% | |
| AGATGTTTCAAAAGCTATCT+TGG | - | chr2.3:3651239-3651258 | MS.gene001663:intron | 30.0% | |
| CATGATTCCAAAAGTAAAAG+AGG | + | chr2.3:3652448-3652467 | None:intergenic | 30.0% | |
| CCTGAATGGTATAAACAAAA+AGG | + | chr2.3:3651785-3651804 | None:intergenic | 30.0% | |
| TACACCATGGCTATTAAATA+CGG | - | chr2.3:3652338-3652357 | MS.gene001663:intron | 30.0% | |
| TATCACTCATAAGTCATAAC+AGG | + | chr2.3:3651887-3651906 | None:intergenic | 30.0% | |
| TGCAAAACAATTTAAGTGCT+TGG | - | chr2.3:3652214-3652233 | MS.gene001663:intron | 30.0% | |
| TTAGGGACTAAGTTAGATAA+AGG | - | chr2.3:3651563-3651582 | MS.gene001663:CDS | 30.0% | |
| TTCGAATAAACCATAGATGT+TGG | - | chr2.3:3651075-3651094 | MS.gene001663:intron | 30.0% | |
| ! | ATGTTCCTTATGTCTCTTTT+AGG | - | chr2.3:3652010-3652029 | MS.gene001663:intron | 30.0% |
| ! | TAGCAATCCTCTTTTACTTT+TGG | - | chr2.3:3652438-3652457 | MS.gene001663:intron | 30.0% |
| ! | TGAAACACAAGAACGATTTT+TGG | - | chr2.3:3652306-3652325 | MS.gene001663:intron | 30.0% |
| !! | ATAAGTTTTGTGAGGCTATT+TGG | + | chr2.3:3651331-3651350 | None:intergenic | 30.0% |
| !! | CCTTTTTGTTTATACCATTC+AGG | - | chr2.3:3651782-3651801 | MS.gene001663:intron | 30.0% |
| !! | GAAAATACTGATTTGTACGA+TGG | - | chr2.3:3652275-3652294 | MS.gene001663:intron | 30.0% |
| !! | TAAGTTTTGTGAGGCTATTT+GGG | + | chr2.3:3651330-3651349 | None:intergenic | 30.0% |
| !!! | CTCTTTTACTTTTGGAATCA+TGG | - | chr2.3:3652446-3652465 | MS.gene001663:intron | 30.0% |
| !!! | GCTTACATAAGCTACTTTTA+TGG | - | chr2.3:3651174-3651193 | MS.gene001663:intron | 30.0% |
| !!! | TAAGCTACTTTTATGGCAAA+TGG | - | chr2.3:3651181-3651200 | MS.gene001663:intron | 30.0% |
| AATCTAATTGGTCAGCTTCT+TGG | - | chr2.3:3652539-3652558 | MS.gene001663:CDS | 35.0% | |
| ACATCTTACATGCCAGTATT+TGG | + | chr2.3:3651994-3652013 | None:intergenic | 35.0% | |
| ACGAAGTTACTGAACACAAA+AGG | + | chr2.3:3652412-3652431 | None:intergenic | 35.0% | |
| ACTTTATCTTCTCAGGTACA+AGG | - | chr2.3:3651920-3651939 | MS.gene001663:intron | 35.0% | |
| AGACAATAAGGCAGTGATTA+TGG | + | chr2.3:3652488-3652507 | None:intergenic | 35.0% | |
| AGAGTTAGAGATCAAACTGA+TGG | - | chr2.3:3651451-3651470 | MS.gene001663:intron | 35.0% | |
| ATGTCCGTATTTAATAGCCA+TGG | + | chr2.3:3652345-3652364 | None:intergenic | 35.0% | |
| CCAATCCTAAAAGAGACATA+AGG | + | chr2.3:3652018-3652037 | None:intergenic | 35.0% | |
| GAACTATAGTTTAGAGACCA+GGG | + | chr2.3:3651391-3651410 | None:intergenic | 35.0% | |
| GGCTTATTTGAGCTTATCTA+CGG | + | chr2.3:3651356-3651375 | None:intergenic | 35.0% | |
| GGTTGAATTGTTCAACAAGT+AGG | - | chr2.3:3650988-3651007 | MS.gene001663:intron | 35.0% | |
| TGAAAAGGAGATGGAGTATA+GGG | - | chr2.3:3650967-3650986 | MS.gene001663:CDS | 35.0% | |
| TGTCAGGTGACTAATCTAAT+TGG | - | chr2.3:3652527-3652546 | MS.gene001663:intron | 35.0% | |
| !! | GTTTGTCACTTTATCTTCTC+AGG | - | chr2.3:3651913-3651932 | MS.gene001663:intron | 35.0% |
| !!! | ATGTCTCTTTTAGGATTGGA+TGG | - | chr2.3:3652019-3652038 | MS.gene001663:intron | 35.0% |
| !!! | CCTTATGTCTCTTTTAGGAT+TGG | - | chr2.3:3652015-3652034 | MS.gene001663:intron | 35.0% |
| !!! | TGGTGTTTTTTGTTGTTGTC+AGG | - | chr2.3:3652511-3652530 | MS.gene001663:intron | 35.0% |
| !!! | TGTCTCTTTTAGGATTGGAT+GGG | - | chr2.3:3652020-3652039 | MS.gene001663:intron | 35.0% |
| AAGGAGTCCGAGCTAAATAT+GGG | - | chr2.3:3651939-3651958 | MS.gene001663:intron | 40.0% | |
| ACACTTATTGAAGCAGGACT+GGG | + | chr2.3:3651812-3651831 | None:intergenic | 40.0% | |
| CGAACTATAGTTTAGAGACC+AGG | + | chr2.3:3651392-3651411 | None:intergenic | 40.0% | |
| CTGAAAAGGAGATGGAGTAT+AGG | - | chr2.3:3650966-3650985 | MS.gene001663:CDS | 40.0% | |
| CTGTCATGTTCTCTTGCTTT+CGG | - | chr2.3:3651658-3651677 | MS.gene001663:intron | 40.0% | |
| GTCAACACACTTATTGAAGC+AGG | + | chr2.3:3651818-3651837 | None:intergenic | 40.0% | |
| ! | GTTTTCGCCCATATTTAGCT+CGG | + | chr2.3:3651949-3651968 | None:intergenic | 40.0% |
| ! | TCACTGCCTTATTGTCTAAC+TGG | - | chr2.3:3652491-3652510 | MS.gene001663:intron | 40.0% |
| !! | CTACGGTCATAAGTTTTGTG+AGG | + | chr2.3:3651339-3651358 | None:intergenic | 40.0% |
| !! | TTGGCTGTTGATTTACACCA+TGG | - | chr2.3:3652325-3652344 | MS.gene001663:intron | 40.0% |
| ACCGCTGTGTTTCCAAATAC+TGG | - | chr2.3:3651979-3651998 | MS.gene001663:intron | 45.0% | |
| ATTGGTCAGCTTCTTGGTAC+TGG | - | chr2.3:3652545-3652564 | MS.gene001663:CDS | 45.0% | |
| CAAGGAGTCCGAGCTAAATA+TGG | - | chr2.3:3651938-3651957 | MS.gene001663:intron | 45.0% | |
| CACACTTATTGAAGCAGGAC+TGG | + | chr2.3:3651813-3651832 | None:intergenic | 45.0% | |
| CCAAACAGGTCCAACATCTA+TGG | + | chr2.3:3651088-3651107 | None:intergenic | 45.0% | |
| CCATAGATGTTGGACCTGTT+TGG | - | chr2.3:3651085-3651104 | MS.gene001663:intron | 45.0% | |
| GCCAGTATTTGGAAACACAG+CGG | + | chr2.3:3651983-3652002 | None:intergenic | 45.0% | |
| TGGAATGGCTGAAAAGGAGA+TGG | - | chr2.3:3650958-3650977 | MS.gene001663:CDS | 45.0% | |
| ! | TTTAGAGACCAGGGCTTGTT+TGG | + | chr2.3:3651382-3651401 | None:intergenic | 45.0% |
| AAGCCAATCCAAACAAGCCC+TGG | - | chr2.3:3651371-3651390 | MS.gene001663:intron | 50.0% | |
| ! | AGACCAGGGCTTGTTTGGAT+TGG | + | chr2.3:3651377-3651396 | None:intergenic | 50.0% |
| CAGGACTGGGTCATCCTGAA+TGG | + | chr2.3:3651799-3651818 | None:intergenic | 55.0% | |
| GGAAACACAGCGGTCAATGC+AGG | + | chr2.3:3651973-3651992 | None:intergenic | 55.0% | |
| ! | GGTCAGCTTCTTGGTACTGG+CGG | - | chr2.3:3652548-3652567 | MS.gene001663:CDS | 55.0% |
| !! | GTCAGCTTCTTGGTACTGGC+GGG | - | chr2.3:3652549-3652568 | MS.gene001663:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 3650953 | 3652580 | 3650953 | ID=MS.gene001663 |
| chr2.3 | mRNA | 3650953 | 3652580 | 3650953 | ID=MS.gene001663.t1;Parent=MS.gene001663 |
| chr2.3 | exon | 3652528 | 3652580 | 3652528 | ID=MS.gene001663.t1.exon1;Parent=MS.gene001663.t1 |
| chr2.3 | CDS | 3652528 | 3652580 | 3652528 | ID=cds.MS.gene001663.t1;Parent=MS.gene001663.t1 |
| chr2.3 | exon | 3651691 | 3651729 | 3651691 | ID=MS.gene001663.t1.exon2;Parent=MS.gene001663.t1 |
| chr2.3 | CDS | 3651691 | 3651729 | 3651691 | ID=cds.MS.gene001663.t1;Parent=MS.gene001663.t1 |
| chr2.3 | exon | 3651529 | 3651598 | 3651529 | ID=MS.gene001663.t1.exon3;Parent=MS.gene001663.t1 |
| chr2.3 | CDS | 3651529 | 3651598 | 3651529 | ID=cds.MS.gene001663.t1;Parent=MS.gene001663.t1 |
| chr2.3 | exon | 3650953 | 3651000 | 3650953 | ID=MS.gene001663.t1.exon4;Parent=MS.gene001663.t1 |
| chr2.3 | CDS | 3650953 | 3651000 | 3650953 | ID=cds.MS.gene001663.t1;Parent=MS.gene001663.t1 |
| Gene Sequence |
| Protein sequence |
