AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00208

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00208.t1 MTR_2g034960 95.881 607 23 2 1 606 1 606 0.0 1173
MS.gene00208.t1 MTR_4g121020 62.225 683 173 12 1 601 2 681 0.0 773
MS.gene00208.t1 MTR_4g121020 62.225 683 172 13 1 601 2 680 0.0 768
MS.gene00208.t1 MTR_4g121020 58.435 575 156 11 107 601 6 577 0.0 595
MS.gene00208.t1 MTR_1g032570 59.091 308 117 3 25 325 8 313 5.98e-115 356
MS.gene00208.t1 MTR_3g106220 48.588 354 157 8 26 356 6 357 3.04e-101 321
MS.gene00208.t1 MTR_3g086100 58.462 260 92 3 28 277 13 266 4.45e-95 303
MS.gene00208.t1 MTR_8g079940 57.874 254 105 1 26 277 23 276 3.05e-92 294
MS.gene00208.t1 MTR_1g013170 44.444 378 174 9 24 385 1 358 1.67e-91 291
MS.gene00208.t1 MTR_7g117705 52.174 276 120 4 24 291 1 272 3.06e-87 279
MS.gene00208.t1 MTR_1g032570 57.724 246 95 3 87 325 1 244 4.39e-85 276
MS.gene00208.t1 MTR_2g450070 53.307 257 107 1 24 267 14 270 1.59e-83 275
MS.gene00208.t1 MTR_2g450070 53.307 257 107 1 24 267 14 270 3.51e-83 275
MS.gene00208.t1 MTR_1g013180 55.000 260 113 2 22 277 150 409 2.97e-82 272
MS.gene00208.t1 MTR_4g131570 42.703 370 172 6 17 368 6 353 1.32e-81 268
MS.gene00208.t1 MTR_4g131600 51.737 259 102 3 22 269 14 260 5.19e-81 266
MS.gene00208.t1 MTR_4g131580 44.706 340 149 8 22 346 11 326 1.44e-77 258
MS.gene00208.t1 MTR_3g102590 46.484 256 86 3 25 277 5 212 8.00e-65 213
MS.gene00208.t1 MTR_3g086100 54.500 200 75 3 87 276 1 194 1.13e-63 218
MS.gene00208.t1 MTR_3g102600 43.284 268 92 3 24 291 4 211 4.24e-63 208
MS.gene00208.t1 MTR_3g102590 47.034 236 76 2 25 259 5 192 9.53e-59 195
MS.gene00208.t1 MTR_3g102600 43.882 237 75 2 25 261 5 183 1.26e-56 189
MS.gene00208.t1 MTR_4g021760 43.096 239 112 5 28 266 11 225 3.87e-56 192
MS.gene00208.t1 MTR_4g021855 40.357 280 135 5 28 304 10 260 1.86e-55 190
MS.gene00208.t1 MTR_4g021790 42.917 240 113 4 28 267 10 225 3.44e-55 188
MS.gene00208.t1 MTR_4g021845 41.810 232 111 2 28 259 10 217 1.49e-51 177
MS.gene00208.t1 MTR_0450s0040 37.770 278 138 7 30 298 6 257 8.14e-48 171
MS.gene00208.t1 MTR_3g102600 40.541 222 72 3 70 291 1 162 1.16e-47 165
MS.gene00208.t1 MTR_6g045327 38.843 242 120 6 27 266 13 228 7.62e-45 164
MS.gene00208.t1 MTR_3g102600 40.625 192 56 2 70 261 1 134 6.12e-42 148
MS.gene00208.t1 MTR_8g105600 38.306 248 133 5 28 267 9 244 1.01e-41 159
MS.gene00208.t1 MTR_7g026400 36.792 212 122 1 58 269 17 216 8.12e-41 157
MS.gene00208.t1 MTR_5g055260 37.083 240 100 6 28 267 10 198 1.71e-37 140
MS.gene00208.t1 MTR_1g013160 47.097 155 64 3 90 233 1 148 4.42e-35 130
MS.gene00208.t1 MTR_8g093040 34.167 240 119 5 27 266 13 213 1.45e-28 115
MS.gene00208.t1 MTR_8g032710 30.000 270 165 6 30 277 19 286 1.59e-27 116
MS.gene00208.t1 MTR_4g023980 35.220 159 80 3 78 236 39 174 5.35e-27 108
MS.gene00208.t1 MTR_4g064730 50.909 110 45 3 186 286 99 208 4.15e-24 103
MS.gene00208.t1 MTR_3g092780 36.250 160 96 3 32 186 56 214 4.79e-23 103
MS.gene00208.t1 MTR_3g092780 36.875 160 94 4 32 186 56 213 3.63e-22 101
MS.gene00208.t1 MTR_3g092780 26.341 410 263 11 32 415 56 452 9.95e-22 100
MS.gene00208.t1 MTR_6g016850 36.220 127 78 2 26 150 7 132 1.77e-21 92.4
MS.gene00208.t1 MTR_7g118260 38.971 136 74 2 29 158 11 143 1.95e-21 99.0
MS.gene00208.t1 MTR_4g061360 35.333 150 91 3 6 150 62 210 4.66e-21 98.2
MS.gene00208.t1 MTR_7g089010 57.971 69 29 0 203 271 84 152 5.10e-21 94.4
MS.gene00208.t1 MTR_7g118260 39.844 128 68 2 29 150 11 135 5.76e-21 97.4
MS.gene00208.t1 MTR_3g092780 26.585 410 261 12 32 415 56 451 6.08e-21 97.8
MS.gene00208.t1 MTR_1g067110 39.344 122 71 1 32 150 85 206 1.38e-20 96.7
MS.gene00208.t1 MTR_1g067110 39.344 122 71 1 32 150 85 206 1.54e-20 96.7
MS.gene00208.t1 MTR_3g037390 35.971 139 84 2 19 152 9 147 2.45e-20 95.5
MS.gene00208.t1 MTR_4g108880 35.507 138 84 2 18 152 21 156 7.70e-19 90.9
MS.gene00208.t1 MTR_8g024260 39.344 122 67 3 34 150 38 157 1.22e-18 90.1
MS.gene00208.t1 MTR_5g014040 37.903 124 68 2 145 268 255 369 9.50e-17 84.0
MS.gene00208.t1 MTR_5g014040 37.008 127 76 2 28 153 14 137 1.02e-13 74.3
MS.gene00208.t1 MTR_1g093080 37.692 130 74 4 198 325 199 323 6.41e-16 80.5
MS.gene00208.t1 MTR_5g041350 55.714 70 24 2 206 274 11 74 6.30e-15 77.0
MS.gene00208.t1 MTR_5g014040 36.434 129 68 3 145 268 215 334 6.82e-15 78.2
MS.gene00208.t1 MTR_5g014040 36.434 129 68 3 145 268 255 374 8.57e-15 77.8
MS.gene00208.t1 MTR_5g014040 37.008 127 76 2 28 153 14 137 1.06e-13 74.3
MS.gene00208.t1 MTR_4g098870 47.887 71 37 0 209 279 309 379 1.06e-14 77.8
MS.gene00208.t1 MTR_4g098870 36.220 127 77 2 28 153 14 137 2.36e-13 73.2
MS.gene00208.t1 MTR_4g078810 41.379 87 46 1 209 295 157 238 1.10e-14 77.0
MS.gene00208.t1 MTR_4g098870 47.887 71 37 0 209 279 309 379 1.13e-14 77.4
MS.gene00208.t1 MTR_4g098870 36.220 127 77 2 28 153 14 137 4.19e-13 72.4
MS.gene00208.t1 MTR_4g098870 47.887 71 37 0 209 279 329 399 1.18e-14 77.4
MS.gene00208.t1 MTR_4g098870 36.220 127 77 2 28 153 34 157 2.99e-13 73.2
MS.gene00208.t1 MTR_4g098870 47.887 71 37 0 209 279 329 399 1.19e-14 77.4
MS.gene00208.t1 MTR_4g098870 36.220 127 77 2 28 153 34 157 4.91e-13 72.4
MS.gene00208.t1 MTR_4g098870 47.887 71 37 0 209 279 348 418 1.32e-14 77.4
MS.gene00208.t1 MTR_4g098870 36.220 127 77 2 28 153 53 176 4.41e-13 72.4
MS.gene00208.t1 MTR_6g088950 35.632 87 56 0 67 153 43 129 5.46e-14 71.6
MS.gene00208.t1 MTR_5g017980 50.704 71 35 0 207 277 189 259 6.08e-14 73.6
MS.gene00208.t1 MTR_5g017980 50.704 71 35 0 207 277 190 260 6.14e-14 73.6
MS.gene00208.t1 MTR_4g081710 55.932 59 25 1 206 263 13 71 1.02e-13 73.2
MS.gene00208.t1 MTR_2g090675 53.333 60 28 0 209 268 160 219 1.04e-13 73.9
MS.gene00208.t1 MTR_2g086450 55.932 59 25 1 206 263 41 99 1.10e-13 73.6
MS.gene00208.t1 MTR_4g086835 51.515 66 32 0 207 272 271 336 1.24e-13 73.6
MS.gene00208.t1 MTR_6g032990 51.667 60 28 1 205 263 37 96 2.68e-13 72.4
MS.gene00208.t1 MTR_6g453220 63.462 52 19 0 227 278 380 431 3.68e-13 72.4
MS.gene00208.t1 MTR_5g054300 30.220 182 115 5 151 325 133 309 5.02e-13 71.2
MS.gene00208.t1 MTR_5g017980 50.000 72 35 1 207 277 190 261 7.30e-13 70.5
MS.gene00208.t1 MTR_6g444980 54.237 59 26 1 206 263 31 89 3.22e-12 68.6
MS.gene00208.t1 MTR_4g086835 50.746 67 32 1 207 272 271 337 3.61e-12 68.9
MS.gene00208.t1 MTR_6g444980 54.237 59 26 1 206 263 31 89 3.76e-12 68.6
MS.gene00208.t1 MTR_7g115530 59.259 54 21 1 210 262 218 271 7.19e-12 67.0
MS.gene00208.t1 MTR_1g090670 52.308 65 27 2 203 263 34 98 1.08e-11 66.6
MS.gene00208.t1 MTR_7g115530 59.259 54 21 1 210 262 218 271 1.10e-11 67.0
MS.gene00208.t1 MTR_7g115530 59.259 54 21 1 210 262 218 271 1.63e-11 67.0
MS.gene00208.t1 MTR_7g115530 59.259 54 21 1 210 262 218 271 1.70e-11 66.6
MS.gene00208.t1 MTR_4g113140 36.283 113 66 3 166 273 188 299 2.58e-11 66.6
MS.gene00208.t1 MTR_7g098250 49.206 63 31 1 206 267 258 320 2.69e-11 66.6
MS.gene00208.t1 MTR_1g080330 50.847 59 28 1 205 262 170 228 5.65e-11 64.3
MS.gene00208.t1 MTR_1g080330 50.847 59 28 1 205 262 170 228 7.71e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00208.t1 AT3G16857 47.059 680 246 20 21 601 26 690 3.31e-175 513
MS.gene00208.t1 AT3G16857 46.423 657 239 19 21 578 26 668 4.92e-164 484
MS.gene00208.t1 AT3G16857 44.724 635 237 20 66 601 1 620 1.56e-144 432
MS.gene00208.t1 AT2G25180 59.924 262 93 3 26 281 11 266 1.03e-95 305
MS.gene00208.t1 AT2G01760 45.263 380 162 8 24 373 3 366 3.23e-95 297
MS.gene00208.t1 AT2G01760 42.157 408 162 9 24 373 3 394 5.46e-89 282
MS.gene00208.t1 AT4G31920 55.000 260 93 2 24 277 9 250 7.09e-87 281
MS.gene00208.t1 AT1G67710 49.702 336 128 9 29 333 8 333 9.28e-86 277
MS.gene00208.t1 AT5G58080 46.875 320 128 7 26 324 12 310 1.54e-78 261
MS.gene00208.t1 AT2G01760 42.271 317 137 9 87 373 1 301 9.61e-62 207
MS.gene00208.t1 AT1G67710 45.683 278 110 9 87 333 1 268 2.89e-56 197
MS.gene00208.t1 AT5G49240 42.915 247 134 3 18 259 28 272 8.10e-54 186
MS.gene00208.t1 AT1G49190 36.364 275 134 6 27 277 29 286 1.08e-45 167
MS.gene00208.t1 AT3G62670 39.407 236 128 5 33 262 42 268 2.08e-45 166
MS.gene00208.t1 AT1G67710 41.473 258 110 9 107 333 12 259 2.15e-40 153
MS.gene00208.t1 AT5G07210 33.955 268 145 6 32 269 16 281 7.85e-39 151
MS.gene00208.t1 AT5G07210 32.971 276 145 7 32 269 16 289 3.25e-36 144
MS.gene00208.t1 AT2G27070 32.103 271 147 7 32 268 16 283 3.55e-33 134
MS.gene00208.t1 AT1G49190 44.800 125 69 0 27 151 29 153 2.61e-29 123
MS.gene00208.t1 AT5G59570 52.083 96 43 1 194 286 125 220 4.13e-25 106
MS.gene00208.t1 AT5G59570 52.083 96 43 1 194 286 125 220 4.13e-25 106
MS.gene00208.t1 AT3G46640 51.064 94 45 1 194 286 128 221 1.76e-23 102
MS.gene00208.t1 AT3G46640 51.064 94 45 1 194 286 128 221 1.76e-23 102
MS.gene00208.t1 AT3G46640 51.064 94 45 1 194 286 128 221 2.53e-23 101
MS.gene00208.t1 AT2G46790 40.650 123 68 2 32 150 37 158 4.71e-23 102
MS.gene00208.t1 AT2G46790 40.650 123 68 2 32 150 37 158 5.22e-23 102
MS.gene00208.t1 AT5G24470 39.200 125 71 2 31 151 49 172 5.12e-21 97.4
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 6.60e-21 95.9
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 6.60e-21 95.9
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 6.60e-21 95.9
MS.gene00208.t1 AT2G40970 60.606 66 26 0 203 268 98 163 8.87e-21 92.4
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 9.85e-21 96.3
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 9.85e-21 96.3
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 9.85e-21 96.3
MS.gene00208.t1 AT5G60100 39.837 123 69 2 32 150 64 185 2.04e-20 95.5
MS.gene00208.t1 AT5G05090 59.091 66 27 0 203 268 74 139 2.49e-20 91.7
MS.gene00208.t1 AT5G61380 38.710 124 73 1 32 152 19 142 4.27e-20 94.7
MS.gene00208.t1 AT3G10760 59.091 66 27 0 203 268 98 163 4.31e-20 92.4
MS.gene00208.t1 AT5G02810 40.164 122 70 1 32 150 78 199 5.61e-20 94.7
MS.gene00208.t1 AT5G44190 50.649 77 38 0 203 279 140 216 8.67e-18 85.9
MS.gene00208.t1 AT5G44190 50.649 77 38 0 203 279 140 216 1.13e-17 85.9
MS.gene00208.t1 AT2G20570 39.823 113 63 2 170 277 108 220 4.60e-16 81.3
MS.gene00208.t1 AT2G20570 38.060 134 77 2 145 277 108 236 2.26e-14 76.3
MS.gene00208.t1 AT3G04030 51.282 78 30 3 206 282 41 111 3.60e-14 75.1
MS.gene00208.t1 AT3G04030 51.282 78 30 3 206 282 41 111 4.06e-14 75.1
MS.gene00208.t1 AT5G18240 50.000 82 37 3 205 285 40 118 5.73e-14 74.7
MS.gene00208.t1 AT5G18240 50.000 82 37 3 205 285 40 118 5.73e-14 74.7
MS.gene00208.t1 AT5G18240 50.000 82 37 3 205 285 40 118 5.87e-14 74.7
MS.gene00208.t1 AT5G18240 50.000 82 37 3 205 285 40 118 6.49e-14 74.3
MS.gene00208.t1 AT5G18240 50.000 82 37 3 205 285 40 118 6.49e-14 74.3
MS.gene00208.t1 AT3G04030 48.052 77 33 2 206 282 41 110 4.75e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 5.46e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.17e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 5.46e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.17e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 5.46e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.17e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 5.46e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.17e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 5.46e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.17e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 5.46e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.17e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 6.64e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 6.64e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 6.64e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 6.64e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MS.gene00208.t1 AT4G18020 36.029 136 78 3 26 154 12 145 6.64e-13 72.0
MS.gene00208.t1 AT4G18020 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MS.gene00208.t1 AT1G69580 54.237 59 26 1 206 263 30 88 7.82e-13 70.5
MS.gene00208.t1 AT1G69580 54.237 59 26 1 206 263 30 88 7.96e-13 70.5
MS.gene00208.t1 AT3G24120 52.308 65 27 2 203 263 31 95 3.38e-12 68.2
MS.gene00208.t1 AT3G24120 52.308 65 27 2 203 263 31 95 3.47e-12 68.2
MS.gene00208.t1 AT1G79430 53.333 60 27 1 205 263 29 88 3.92e-12 68.6
MS.gene00208.t1 AT3G25790 38.655 119 61 4 214 330 200 308 7.17e-12 67.8
MS.gene00208.t1 AT4G13640 52.308 65 27 2 203 263 27 91 7.61e-12 67.0
MS.gene00208.t1 AT4G13640 52.308 65 27 2 203 263 27 91 7.85e-12 67.0
MS.gene00208.t1 AT1G49560 36.283 113 64 2 209 313 191 303 2.79e-11 65.9
MS.gene00208.t1 AT3G12730 49.275 69 28 2 202 263 9 77 2.98e-11 64.3
MS.gene00208.t1 AT1G25550 45.070 71 38 1 198 267 197 267 4.02e-11 65.5
MS.gene00208.t1 AT5G29000 45.714 70 35 2 196 262 215 284 5.92e-11 64.3
MS.gene00208.t1 AT1G68670 45.679 81 42 2 194 272 198 278 6.08e-11 64.7
MS.gene00208.t1 AT2G03500 47.059 68 35 1 207 273 230 297 8.01e-11 65.1

Find 138 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCTTCATCAACAGTTTA+TGG 0.134096 2.1:+57874161 MS.gene00208:CDS
CTTCAAACCCTTTCAGCTTC+TGG 0.197138 2.1:+57875046 MS.gene00208:CDS
CCTTATCTCCGACCAGTTTC+CGG 0.220153 2.1:+57873109 MS.gene00208:CDS
GTTGTTGTTGTTGAGTTTCA+TGG 0.237423 2.1:-57873042 None:intergenic
GAAGCTACTTCGGGAATTAA+AGG 0.286940 2.1:+57875499 MS.gene00208:CDS
TATGGATGGATTCAAACTTT+TGG 0.295920 2.1:+57873669 MS.gene00208:CDS
AGTACGCCAGAGTCAATATT+TGG 0.308653 2.1:+57874998 MS.gene00208:CDS
CACCAGTCTGCTCAATCCTT+TGG 0.321223 2.1:+57875241 MS.gene00208:CDS
TTGAAGATCAATTTCATTTA+CGG 0.326626 2.1:-57875029 None:intergenic
GAGAACTCAAAACTAAGATT+TGG 0.328580 2.1:+57875124 MS.gene00208:CDS
TTCAGCTTCTGGCCAGTTCT+CGG 0.333161 2.1:+57875057 MS.gene00208:CDS
ACTTCGGGAATTAAAGGATT+AGG 0.335057 2.1:+57875505 MS.gene00208:CDS
AAGAAGATGAAGCAGATGAT+AGG 0.337750 2.1:+57874094 MS.gene00208:CDS
TGCTTTGGCTCAATGTTGAT+AGG 0.342601 2.1:-57875205 None:intergenic
GATAATGAGTTTGACTTTGA+TGG 0.349646 2.1:+57876007 MS.gene00208:CDS
TTGAGCAGACTGGTGAAAAT+TGG 0.352834 2.1:-57875233 None:intergenic
TGAGGTGTTGACCAACATTA+TGG 0.359965 2.1:-57875735 None:intergenic
TGAAAATTGGCAAACTGCTT+TGG 0.368522 2.1:-57875220 None:intergenic
CGACGTGCATATGCCTGATA+TGG 0.368808 2.1:+57873651 MS.gene00208:CDS
TCGAGGCTTTGAAGAATATA+TGG 0.369544 2.1:+57873920 MS.gene00208:CDS
CTTCGGGAATTAAAGGATTA+GGG 0.371380 2.1:+57875506 MS.gene00208:CDS
AAACAATGCTTACTAATAAC+AGG 0.383212 2.1:-57873718 None:intergenic
CTATTGACTGATATTCCATT+TGG 0.387671 2.1:-57875415 None:intergenic
CACGGAGACCCGCCGGAAAC+TGG 0.388410 2.1:-57873121 None:intergenic
TTCGGAAGAGAAAGAATAAC+TGG 0.397525 2.1:+57873953 MS.gene00208:CDS
TCATTTAGCATCTGACCTCT+TGG 0.399993 2.1:-57875337 None:intergenic
CTAGACTTTGCACCGAGAAC+TGG 0.401730 2.1:-57875069 None:intergenic
TAACAGGATCTGTTATAAAC+TGG 0.403457 2.1:-57876049 None:intergenic
AAACGTTGCCAGTCATCTTC+AGG 0.403511 2.1:+57874535 MS.gene00208:CDS
AACCTCAGCTCTCTTGCATT+TGG 0.403620 2.1:-57873578 None:intergenic
ATTTGCTAATCCCCAATCAT+TGG 0.406561 2.1:-57875569 None:intergenic
CAAAGAAGCCTCGAGTTGTT+TGG 0.412556 2.1:+57874133 MS.gene00208:CDS
TTTCATGCGGAAGCTACTTC+GGG 0.416515 2.1:+57875490 MS.gene00208:CDS
CCATCAGAAGTCCAATGATT+GGG 0.416956 2.1:+57875558 MS.gene00208:CDS
TATTCAAGTTACTTTGCTGC+TGG 0.418842 2.1:-57874964 None:intergenic
ATGGAAATGATGAATGTTCC+TGG 0.426157 2.1:+57874500 MS.gene00208:CDS
AGTTTGAATCCATCCATATC+AGG 0.428415 2.1:-57873664 None:intergenic
GTTTCATGCGGAAGCTACTT+CGG 0.458202 2.1:+57875489 MS.gene00208:CDS
ATAAGGTTGCCGGAGTTTGA+TGG 0.460200 2.1:-57873092 None:intergenic
TATGGCTGTTGTGAATCAAT+TGG 0.460490 2.1:+57874179 MS.gene00208:CDS
TCCATCAGAAGTCCAATGAT+TGG 0.463174 2.1:+57875557 MS.gene00208:CDS
TGATTAGAAAGAGAGCTAAC+AGG 0.464856 2.1:-57876065 None:intergenic
TGGAAAGATCTGAAGCAATC+TGG 0.465336 2.1:+57873973 MS.gene00208:CDS
TTCTCGCAGCACAAAGGCGT+TGG 0.468445 2.1:+57875977 MS.gene00208:intron
CCGGAAACTGGTCGGAGATA+AGG 0.470882 2.1:-57873109 None:intergenic
CCCAATCATTGGACTTCTGA+TGG 0.470889 2.1:-57875558 None:intergenic
TTGTCGAGTCTGTTGGATAG+AGG 0.472904 2.1:-57875683 None:intergenic
ATGGCTGTTGTGAATCAATT+GGG 0.473696 2.1:+57874180 MS.gene00208:CDS
ATGCAACCTACAGGACCAAA+TGG 0.473881 2.1:+57875400 MS.gene00208:CDS
ACATATGAGCAGCAGTAAAC+CGG 0.474063 2.1:+57875162 MS.gene00208:CDS
CTAGCCAAGCTTCAAGCAGC+AGG 0.477941 2.1:+57875088 MS.gene00208:CDS
GGAACATATTGGATTGGAGA+TGG 0.480393 2.1:+57873690 MS.gene00208:CDS
AGAAGATGAAGCAGATGATA+GGG 0.481453 2.1:+57874095 MS.gene00208:CDS
AGATCAATGTTGCCTTGTAA+AGG 0.489412 2.1:-57875628 None:intergenic
TTCCCTCTTAGATGCACTCC+GGG 0.489466 2.1:+57875439 MS.gene00208:CDS
GTAGGTTGCATTAAAGATGA+TGG 0.494028 2.1:-57875388 None:intergenic
CAACATGCAAATCCTTTACA+AGG 0.495519 2.1:+57875616 MS.gene00208:CDS
TACTCATCTTCAACGAATGA+AGG 0.499328 2.1:+57874045 MS.gene00208:CDS
GATATTCCATTTGGTCCTGT+AGG 0.499380 2.1:-57875406 None:intergenic
GCTTTGGCTCAATGTTGATA+GGG 0.500235 2.1:-57875204 None:intergenic
CAAAACTAAGATTTGGAGAT+GGG 0.507173 2.1:+57875131 MS.gene00208:CDS
CAAGGATCATGAGGCAAGTA+GGG 0.508406 2.1:-57873163 None:intergenic
CTGGTCGGAGATAAGGTTGC+CGG 0.509887 2.1:-57873102 None:intergenic
ATTCAAGTTACTTTGCTGCT+GGG 0.515195 2.1:-57874963 None:intergenic
GTTGAGAAGCATCATATGTC+AGG 0.515544 2.1:-57875597 None:intergenic
CATGAGCGTATTTCTGAACC+AGG 0.515894 2.1:+57875861 MS.gene00208:CDS
GATTGGGGATTAGCAAATAC+AGG 0.520281 2.1:+57875574 MS.gene00208:CDS
TTCAAAGCCTCGATTCGCAC+AGG 0.523205 2.1:-57873910 None:intergenic
ATATATGGCAGCATGTGGTT+CGG 0.524503 2.1:+57873935 MS.gene00208:CDS
TCAAGGATCATGAGGCAAGT+AGG 0.525643 2.1:-57873164 None:intergenic
ATATCCAGTAGCAGTACAAA+AGG 0.527621 2.1:+57875463 MS.gene00208:CDS
TGACTGACTTGATCAGAGAA+GGG 0.534175 2.1:-57875829 None:intergenic
AAACCGGTGAACTTTCTTCA+CGG 0.535622 2.1:+57875178 MS.gene00208:CDS
ATACAAATTCTCATGCTACA+AGG 0.536556 2.1:+57875362 MS.gene00208:CDS
TCAAAACTAAGATTTGGAGA+TGG 0.536886 2.1:+57875130 MS.gene00208:CDS
TTGACTGACTTGATCAGAGA+AGG 0.537467 2.1:-57875830 None:intergenic
TTGTATCTTCGAAGGTTGAG+TGG 0.537963 2.1:+57874935 MS.gene00208:CDS
TGTCGACAAAAGTTGTATTG+AGG 0.539424 2.1:-57875753 None:intergenic
GAACTGGCCAGAAGCTGAAA+GGG 0.541735 2.1:-57875053 None:intergenic
ATCTCCGACCAGTTTCCGGC+GGG 0.544362 2.1:+57873113 MS.gene00208:CDS
TCGCATATTTGCGCTGCCAA+AGG 0.548078 2.1:-57875257 None:intergenic
AATACCGTTTGTATCTTCGA+AGG 0.548651 2.1:+57874927 MS.gene00208:CDS
AGAACTGGCCAGAAGCTGAA+AGG 0.554761 2.1:-57875054 None:intergenic
TCATCTTTAATGCAACCTAC+AGG 0.556863 2.1:+57875391 MS.gene00208:CDS
TTAGTGATGTCGGCTGATGA+TGG 0.559693 2.1:+57873832 MS.gene00208:intron
ATACCCGGAGTGCATCTAAG+AGG 0.561462 2.1:-57875442 None:intergenic
ATATCGTACCTGAAGATGAC+TGG 0.562314 2.1:-57874543 None:intergenic
AAGCCCTTGGTTGATGCAGA+TGG 0.564484 2.1:+57875303 MS.gene00208:CDS
CTCTGATCAAGTCAGTCAAG+AGG 0.573459 2.1:+57875834 MS.gene00208:CDS
TTTGTCGACAATTCATTAAG+GGG 0.574449 2.1:+57875766 MS.gene00208:CDS
TTGATCTCAATGTTTGTACT+CGG 0.576687 2.1:-57875805 None:intergenic
ATTCCGTGAAGAAAGTTCAC+CGG 0.576781 2.1:-57875181 None:intergenic
GTTCCTCCAAATATTGACTC+TGG 0.578483 2.1:-57875004 None:intergenic
TGCCAAAGGATTGAGCAGAC+TGG 0.579512 2.1:-57875243 None:intergenic
TTTCCCTCTTAGATGCACTC+CGG 0.583189 2.1:+57875438 MS.gene00208:CDS
TGACTCTGGCGTACTCATGA+AGG 0.583909 2.1:-57874990 None:intergenic
GCAAGAGCTCAAAGAAGAGG+AGG 0.587165 2.1:+57874067 MS.gene00208:CDS
GACTGACTTGATCAGAGAAG+GGG 0.587939 2.1:-57875828 None:intergenic
ACAAGTACACGGAGACCCGC+CGG 0.589774 2.1:-57873128 None:intergenic
CAAGGTGATCACCATAATGT+TGG 0.591086 2.1:+57875724 MS.gene00208:CDS
AAGTCCTGCTGCTTGAAGCT+TGG 0.592461 2.1:-57875092 None:intergenic
AGATGAGTAATTAGCATCAG+TGG 0.594629 2.1:-57874031 None:intergenic
CTGCTGCAAAGAGAAGCCCT+TGG 0.595593 2.1:+57875290 MS.gene00208:CDS
TGGTGAAGTTGAGGTTGACA+TGG 0.599570 2.1:-57873072 None:intergenic
AAGATCAATTTCATTTACGG+AGG 0.607064 2.1:-57875026 None:intergenic
GAGCCATCTGCATCAACCAA+GGG 0.609724 2.1:-57875306 None:intergenic
TGAGCCATCTGCATCAACCA+AGG 0.610874 2.1:-57875307 None:intergenic
AATAGAGATGGTATGAGTCA+AGG 0.612883 2.1:+57875706 MS.gene00208:CDS
TCAACCTTCGAAGATACAAA+CGG 0.613662 2.1:-57874931 None:intergenic
AATCAAACCTGTGCGAATCG+AGG 0.614923 2.1:+57873903 MS.gene00208:CDS
TCAACTTCACCATCAAACTC+CGG 0.617812 2.1:+57873083 MS.gene00208:CDS
TACCCGGAGTGCATCTAAGA+GGG 0.617885 2.1:-57875441 None:intergenic
GTGCATATGCCTGATATGGA+TGG 0.621582 2.1:+57873655 MS.gene00208:CDS
CAGACTCGACAAAATAGAGA+TGG 0.622886 2.1:+57875694 MS.gene00208:CDS
CATCAGAAGTCCAATGATTG+GGG 0.623338 2.1:+57875559 MS.gene00208:CDS
AAGATTGAAGACCTTCATAG+AGG 0.625198 2.1:-57873205 None:intergenic
GCATTCTTTCAAGGATCATG+AGG 0.625199 2.1:-57873172 None:intergenic
TGGAAATGATGAATGTTCCT+GGG 0.628797 2.1:+57874501 MS.gene00208:CDS
AAAGATCTGAAGCAATCTGG+TGG 0.628824 2.1:+57873976 MS.gene00208:CDS
TATCTCCGACCAGTTTCCGG+CGG 0.629363 2.1:+57873112 MS.gene00208:CDS
GAAGAATATATGGCAGCATG+TGG 0.629609 2.1:+57873930 MS.gene00208:CDS
TACAAAAGGCGTGTTTCATG+CGG 0.631885 2.1:+57875477 MS.gene00208:CDS
TTCGGGAATTAAAGGATTAG+GGG 0.632056 2.1:+57875507 MS.gene00208:CDS
TTGTATTTCTCGCAGCACAA+AGG 0.634173 2.1:+57875971 MS.gene00208:intron
CCACGATGGCCTAAAATCGA+CGG 0.634519 2.1:-57875655 None:intergenic
AAGGCAAGAGCTCAAAGAAG+AGG 0.635070 2.1:+57874064 MS.gene00208:CDS
TCGGGAATTAAAGGATTAGG+GGG 0.636049 2.1:+57875508 MS.gene00208:CDS
ACGCCAGAGTCAATATTTGG+AGG 0.638077 2.1:+57875001 MS.gene00208:CDS
TCAATAGACCAAACAACTCG+AGG 0.639848 2.1:-57874141 None:intergenic
GGAGTTTGATGGTGAAGTTG+AGG 0.651951 2.1:-57873081 None:intergenic
CATCATCAACAACAAGTACA+CGG 0.653506 2.1:-57873139 None:intergenic
GAGACCCGCCGGAAACTGGT+CGG 0.661705 2.1:-57873117 None:intergenic
CAAGAGCTCAAAGAAGAGGA+GGG 0.663100 2.1:+57874068 MS.gene00208:CDS
TACCAAATGCAAGAGAGCTG+AGG 0.664673 2.1:+57873576 MS.gene00208:CDS
GTACTGCTACTGGATATACC+CGG 0.685345 2.1:-57875457 None:intergenic
ATGGCTCAGTCTCAACCAAG+AGG 0.702081 2.1:+57875322 MS.gene00208:CDS
GTAATGAAAGGTGTGACACA+CGG 0.719143 2.1:+57873865 MS.gene00208:CDS
ATAAACTGGAATGTTGTCTG+CGG 0.732217 2.1:-57876035 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTGAACTAAAAATAAAAA+TGG - chr2.1:57873365-57873384 None:intergenic 15.0%
!! TAAGGAAACATAAATATTTA+GGG - chr2.1:57875908-57875927 None:intergenic 15.0%
!! TCTGAACTAAAAATAAAAAT+GGG - chr2.1:57873364-57873383 None:intergenic 15.0%
!!! AATAAATGATTTGTCTATAA+AGG - chr2.1:57875936-57875955 None:intergenic 15.0%
!!! AGAAATATTTAAGATTCTAT+GGG - chr2.1:57874382-57874401 None:intergenic 15.0%
!! AAAAAACTCAATCAAACAAA+AGG - chr2.1:57873333-57873352 None:intergenic 20.0%
!! AAAAACTCAATCAAACAAAA+GGG - chr2.1:57873332-57873351 None:intergenic 20.0%
!! CAAGGATATAACATAATTAA+GGG - chr2.1:57874222-57874241 None:intergenic 20.0%
!! CTTAACAATAACAAAAATAG+AGG - chr2.1:57874587-57874606 None:intergenic 20.0%
!! CTTCTAAACTAACTTATTAT+AGG - chr2.1:57874634-57874653 None:intergenic 20.0%
!! GTAAGGAAACATAAATATTT+AGG - chr2.1:57875909-57875928 None:intergenic 20.0%
!! TTGAAGATCAATTTCATTTA+CGG - chr2.1:57875032-57875051 None:intergenic 20.0%
!!! AAATATTTAAGATTCTATGG+GGG - chr2.1:57874380-57874399 None:intergenic 20.0%
!!! ATAATAAGTTAGTTTAGAAG+TGG + chr2.1:57874634-57874653 MS.gene00208:intron 20.0%
!!! ATCATTTCCATAATCTTTTT+AGG - chr2.1:57874491-57874510 None:intergenic 20.0%
!!! CAGAAATATTTAAGATTCTA+TGG - chr2.1:57874383-57874402 None:intergenic 20.0%
!!! GAAATATTTAAGATTCTATG+GGG - chr2.1:57874381-57874400 None:intergenic 20.0%
!!! GCTTTTGATATGATTTAATA+TGG + chr2.1:57873417-57873436 MS.gene00208:intron 20.0%
!!! TAAGTGTTTTTTTCAACTAT+TGG + chr2.1:57873453-57873472 MS.gene00208:intron 20.0%
!!! TTATTTTTAGTTCAGATTCT+TGG + chr2.1:57873368-57873387 MS.gene00208:intron 20.0%
!!! TTTTAACACTAATTCATGAT+GGG - chr2.1:57873250-57873269 None:intergenic 20.0%
!!! TTTTTAACACTAATTCATGA+TGG - chr2.1:57873251-57873270 None:intergenic 20.0%
! AAACAATGCTTACTAATAAC+AGG - chr2.1:57873721-57873740 None:intergenic 25.0%
! AAATTGATATTGATATGCAG+AGG + chr2.1:57874460-57874479 MS.gene00208:intron 25.0%
! AGCATAATTCAAAAGAAAGA+TGG - chr2.1:57873400-57873419 None:intergenic 25.0%
! ATATCTCGGAAAAACTTTAA+GGG - chr2.1:57874308-57874327 None:intergenic 25.0%
! CTTCTTAGAGAGAATAAAAA+CGG + chr2.1:57873610-57873629 MS.gene00208:CDS 25.0%
! GCAAGGATATAACATAATTA+AGG - chr2.1:57874223-57874242 None:intergenic 25.0%
! TATATCTCGGAAAAACTTTA+AGG - chr2.1:57874309-57874328 None:intergenic 25.0%
! TTCTTAGAGAGAATAAAAAC+GGG + chr2.1:57873611-57873630 MS.gene00208:CDS 25.0%
! TTGTCTATAAAGGAAAAGTA+AGG - chr2.1:57875926-57875945 None:intergenic 25.0%
!! AAAGTCTCAAACTTTAACTT+TGG - chr2.1:57873487-57873506 None:intergenic 25.0%
!! TTTTGTCGACAATTCATTAA+GGG + chr2.1:57875765-57875784 MS.gene00208:CDS 25.0%
!!! TTCAAACTTTTGGAACATAT+TGG + chr2.1:57873679-57873698 MS.gene00208:CDS 25.0%
AACAATCTTGTAGAATCACA+AGG - chr2.1:57874334-57874353 None:intergenic 30.0%
AAGATCAATTTCATTTACGG+AGG - chr2.1:57875029-57875048 None:intergenic 30.0%
ACAAGCAAAACAGAATCAAA+TGG - chr2.1:57873537-57873556 None:intergenic 30.0%
ACTGTAACTCTTAACATTCA+AGG - chr2.1:57874845-57874864 None:intergenic 30.0%
ATACAAATTCTCATGCTACA+AGG + chr2.1:57875362-57875381 MS.gene00208:CDS 30.0%
CAAAACTAAGATTTGGAGAT+GGG + chr2.1:57875131-57875150 MS.gene00208:CDS 30.0%
GAATCACAAGGATTATATCT+CGG - chr2.1:57874322-57874341 None:intergenic 30.0%
GAGAACTCAAAACTAAGATT+TGG + chr2.1:57875124-57875143 MS.gene00208:CDS 30.0%
TAACAGGATCTGTTATAAAC+TGG - chr2.1:57876052-57876071 None:intergenic 30.0%
TCAAAACTAAGATTTGGAGA+TGG + chr2.1:57875130-57875149 MS.gene00208:CDS 30.0%
TTGATCTCAATGTTTGTACT+CGG - chr2.1:57875808-57875827 None:intergenic 30.0%
TTTGTCGACAATTCATTAAG+GGG + chr2.1:57875766-57875785 MS.gene00208:CDS 30.0%
! AATATTTAGGGTCAACAGAA+AGG - chr2.1:57875896-57875915 None:intergenic 30.0%
! ATAAGTTAGTTTAGAAGTGG+TGG + chr2.1:57874637-57874656 MS.gene00208:intron 30.0%
! CTATTGACTGATATTCCATT+TGG - chr2.1:57875418-57875437 None:intergenic 30.0%
! CTTTTGTCGACAATTCATTA+AGG + chr2.1:57875764-57875783 MS.gene00208:CDS 30.0%
! GATAATGAGTTTGACTTTGA+TGG + chr2.1:57876007-57876026 MS.gene00208:CDS 30.0%
! TGAAAGAGAACATTTTTGCA+AGG - chr2.1:57874240-57874259 None:intergenic 30.0%
! TTGATTCTGTTTTGCTTGTT+GGG + chr2.1:57873538-57873557 MS.gene00208:intron 30.0%
! TTTGATTCTGTTTTGCTTGT+TGG + chr2.1:57873537-57873556 MS.gene00208:intron 30.0%
!! CGTACAAACTGATTTTGTAA+GGG + chr2.1:57874671-57874690 MS.gene00208:intron 30.0%
!! TATGGATGGATTCAAACTTT+TGG + chr2.1:57873669-57873688 MS.gene00208:CDS 30.0%
!! TCATCTTTGTTTGTTTTCCA+CGG + chr2.1:57874257-57874276 MS.gene00208:intron 30.0%
!! TCGTACAAACTGATTTTGTA+AGG + chr2.1:57874670-57874689 MS.gene00208:intron 30.0%
!!! AATCTTAGTTTTGAGTTCTC+AGG - chr2.1:57875124-57875143 None:intergenic 30.0%
!!! ACTTTTGGAACATATTGGAT+TGG + chr2.1:57873684-57873703 MS.gene00208:CDS 30.0%
!!! TGAATGTGTTTTAGTGATGT+CGG + chr2.1:57873822-57873841 MS.gene00208:intron 30.0%
AAGAAGATGAAGCAGATGAT+AGG + chr2.1:57874094-57874113 MS.gene00208:CDS 35.0%
AAGATTGAAGACCTTCATAG+AGG - chr2.1:57873208-57873227 None:intergenic 35.0%
AATACCGTTTGTATCTTCGA+AGG + chr2.1:57874927-57874946 MS.gene00208:CDS 35.0%
AATAGAGATGGTATGAGTCA+AGG + chr2.1:57875706-57875725 MS.gene00208:CDS 35.0%
ACTTCGGGAATTAAAGGATT+AGG + chr2.1:57875505-57875524 MS.gene00208:CDS 35.0%
AGAAGATGAAGCAGATGATA+GGG + chr2.1:57874095-57874114 MS.gene00208:CDS 35.0%
AGAGCCTATATAAGATCTCA+TGG + chr2.1:57874722-57874741 MS.gene00208:intron 35.0%
AGATCAATGTTGCCTTGTAA+AGG - chr2.1:57875631-57875650 None:intergenic 35.0%
AGATGAGTAATTAGCATCAG+TGG - chr2.1:57874034-57874053 None:intergenic 35.0%
AGTTTGAATCCATCCATATC+AGG - chr2.1:57873667-57873686 None:intergenic 35.0%
ATAAACTGGAATGTTGTCTG+CGG - chr2.1:57876038-57876057 None:intergenic 35.0%
ATATCCAGTAGCAGTACAAA+AGG + chr2.1:57875463-57875482 MS.gene00208:CDS 35.0%
ATGGAAATGATGAATGTTCC+TGG + chr2.1:57874500-57874519 MS.gene00208:CDS 35.0%
ATGGCTGTTGTGAATCAATT+GGG + chr2.1:57874180-57874199 MS.gene00208:CDS 35.0%
CAAAATCAGTTTGTACGATG+AGG - chr2.1:57874668-57874687 None:intergenic 35.0%
CAACATGCAAATCCTTTACA+AGG + chr2.1:57875616-57875635 MS.gene00208:CDS 35.0%
CATCATCAACAACAAGTACA+CGG - chr2.1:57873142-57873161 None:intergenic 35.0%
CTTCGGGAATTAAAGGATTA+GGG + chr2.1:57875506-57875525 MS.gene00208:CDS 35.0%
GAGCCTATATAAGATCTCAT+GGG + chr2.1:57874723-57874742 MS.gene00208:intron 35.0%
GGCTGTTCCTAAAAAGATTA+TGG + chr2.1:57874481-57874500 MS.gene00208:CDS 35.0%
GTAACTCTTAACATTCAAGG+TGG - chr2.1:57874842-57874861 None:intergenic 35.0%
GTAGGTTGCATTAAAGATGA+TGG - chr2.1:57875391-57875410 None:intergenic 35.0%
TAAGATTCTATGGGGGAAAA+GGG - chr2.1:57874373-57874392 None:intergenic 35.0%
TACTCATCTTCAACGAATGA+AGG + chr2.1:57874045-57874064 MS.gene00208:CDS 35.0%
TATGGCTGTTGTGAATCAAT+TGG + chr2.1:57874179-57874198 MS.gene00208:CDS 35.0%
TCAACCTTCGAAGATACAAA+CGG - chr2.1:57874934-57874953 None:intergenic 35.0%
TCATCTTTAATGCAACCTAC+AGG + chr2.1:57875391-57875410 MS.gene00208:CDS 35.0%
TGAAAATTGGCAAACTGCTT+TGG - chr2.1:57875223-57875242 None:intergenic 35.0%
TGAGCTTCATCAACAGTTTA+TGG + chr2.1:57874161-57874180 MS.gene00208:CDS 35.0%
TGGAAATGATGAATGTTCCT+GGG + chr2.1:57874501-57874520 MS.gene00208:CDS 35.0%
TGTCGACAAAAGTTGTATTG+AGG - chr2.1:57875756-57875775 None:intergenic 35.0%
TTAAGATTCTATGGGGGAAA+AGG - chr2.1:57874374-57874393 None:intergenic 35.0%
TTCGGAAGAGAAAGAATAAC+TGG + chr2.1:57873953-57873972 MS.gene00208:CDS 35.0%
TTCGGGAATTAAAGGATTAG+GGG + chr2.1:57875507-57875526 MS.gene00208:CDS 35.0%
! AAAAACGGAGCATTCTTTCA+AGG - chr2.1:57873184-57873203 None:intergenic 35.0%
! ATTCAAGTTACTTTGCTGCT+GGG - chr2.1:57874966-57874985 None:intergenic 35.0%
! ATTTGCTAATCCCCAATCAT+TGG - chr2.1:57875572-57875591 None:intergenic 35.0%
! GGAAAAAACGTGGTAATGAA+AGG + chr2.1:57873853-57873872 MS.gene00208:CDS 35.0%
! TATTCAAGTTACTTTGCTGC+TGG - chr2.1:57874967-57874986 None:intergenic 35.0%
! TCGGAAAAACTTTAAGGGAA+TGG - chr2.1:57874303-57874322 None:intergenic 35.0%
!! TCGAGGCTTTGAAGAATATA+TGG + chr2.1:57873920-57873939 MS.gene00208:CDS 35.0%
AAACCGGTGAACTTTCTTCA+CGG + chr2.1:57875178-57875197 MS.gene00208:CDS 40.0%
ACATATGAGCAGCAGTAAAC+CGG + chr2.1:57875162-57875181 MS.gene00208:CDS 40.0%
AGGTCATATCTCATCTAACG+TGG - chr2.1:57874614-57874633 None:intergenic 40.0%
AGTACGCCAGAGTCAATATT+TGG + chr2.1:57874998-57875017 MS.gene00208:CDS 40.0%
ATATATGGCAGCATGTGGTT+CGG + chr2.1:57873935-57873954 MS.gene00208:CDS 40.0%
ATATCGTACCTGAAGATGAC+TGG - chr2.1:57874546-57874565 None:intergenic 40.0%
ATGTCCACTTATCAGTGTGA+GGG + chr2.1:57874795-57874814 MS.gene00208:intron 40.0%
ATTCCGTGAAGAAAGTTCAC+CGG - chr2.1:57875184-57875203 None:intergenic 40.0%
CAAGGTGATCACCATAATGT+TGG + chr2.1:57875724-57875743 MS.gene00208:CDS 40.0%
CAGACTCGACAAAATAGAGA+TGG + chr2.1:57875694-57875713 MS.gene00208:CDS 40.0%
CATCAGAAGTCCAATGATTG+GGG + chr2.1:57875559-57875578 MS.gene00208:CDS 40.0%
CCATCAGAAGTCCAATGATT+GGG + chr2.1:57875558-57875577 MS.gene00208:CDS 40.0%
GAAGAATATATGGCAGCATG+TGG + chr2.1:57873930-57873949 MS.gene00208:CDS 40.0%
GAAGCTACTTCGGGAATTAA+AGG + chr2.1:57875499-57875518 MS.gene00208:CDS 40.0%
GACCTTCATAGAGGCAAAAA+CGG - chr2.1:57873199-57873218 None:intergenic 40.0%
GATATTCCATTTGGTCCTGT+AGG - chr2.1:57875409-57875428 None:intergenic 40.0%
GCATTCTTTCAAGGATCATG+AGG - chr2.1:57873175-57873194 None:intergenic 40.0%
GGTCATATCTCATCTAACGT+GGG - chr2.1:57874613-57874632 None:intergenic 40.0%
TCAACTTCACCATCAAACTC+CGG + chr2.1:57873083-57873102 MS.gene00208:CDS 40.0%
TCAATAGACCAAACAACTCG+AGG - chr2.1:57874144-57874163 None:intergenic 40.0%
TCATTTAGCATCTGACCTCT+TGG - chr2.1:57875340-57875359 None:intergenic 40.0%
TCCATCAGAAGTCCAATGAT+TGG + chr2.1:57875557-57875576 MS.gene00208:CDS 40.0%
TCGGGAATTAAAGGATTAGG+GGG + chr2.1:57875508-57875527 MS.gene00208:CDS 40.0%
TGGCCCATGAGATCTTATAT+AGG - chr2.1:57874729-57874748 None:intergenic 40.0%
TTGAGCAGACTGGTGAAAAT+TGG - chr2.1:57875236-57875255 None:intergenic 40.0%
TTGTATCTTCGAAGGTTGAG+TGG + chr2.1:57874935-57874954 MS.gene00208:CDS 40.0%
TTGTATTTCTCGCAGCACAA+AGG + chr2.1:57875971-57875990 MS.gene00208:intron 40.0%
! AAAGATCTGAAGCAATCTGG+TGG + chr2.1:57873976-57873995 MS.gene00208:CDS 40.0%
! CTCTATTTTGTCGAGTCTGT+TGG - chr2.1:57875693-57875712 None:intergenic 40.0%
! GATTGGGGATTAGCAAATAC+AGG + chr2.1:57875574-57875593 MS.gene00208:CDS 40.0%
! GCTTTGGCTCAATGTTGATA+GGG - chr2.1:57875207-57875226 None:intergenic 40.0%
! GGAACATATTGGATTGGAGA+TGG + chr2.1:57873690-57873709 MS.gene00208:CDS 40.0%
! GTAATGAAAGGTGTGACACA+CGG + chr2.1:57873865-57873884 MS.gene00208:CDS 40.0%
! TACAAAAGGCGTGTTTCATG+CGG + chr2.1:57875477-57875496 MS.gene00208:CDS 40.0%
! TGCTTTGGCTCAATGTTGAT+AGG - chr2.1:57875208-57875227 None:intergenic 40.0%
! TGGAAAGATCTGAAGCAATC+TGG + chr2.1:57873973-57873992 MS.gene00208:CDS 40.0%
!! GTTCCTCCAAATATTGACTC+TGG - chr2.1:57875007-57875026 None:intergenic 40.0%
!! GTTGAGAAGCATCATATGTC+AGG - chr2.1:57875600-57875619 None:intergenic 40.0%
!! TGACTGACTTGATCAGAGAA+GGG - chr2.1:57875832-57875851 None:intergenic 40.0%
!! TGAGGTGTTGACCAACATTA+TGG - chr2.1:57875738-57875757 None:intergenic 40.0%
!! TTGACTGACTTGATCAGAGA+AGG - chr2.1:57875833-57875852 None:intergenic 40.0%
AAACGTTGCCAGTCATCTTC+AGG + chr2.1:57874535-57874554 MS.gene00208:CDS 45.0%
AACCTCAGCTCTCTTGCATT+TGG - chr2.1:57873581-57873600 None:intergenic 45.0%
AAGGCAAGAGCTCAAAGAAG+AGG + chr2.1:57874064-57874083 MS.gene00208:CDS 45.0%
AATCAAACCTGTGCGAATCG+AGG + chr2.1:57873903-57873922 MS.gene00208:CDS 45.0%
ACAGAAAGGAAGAACCAACC+TGG - chr2.1:57875882-57875901 None:intergenic 45.0%
ACGCCAGAGTCAATATTTGG+AGG + chr2.1:57875001-57875020 MS.gene00208:CDS 45.0%
ATAAGGTTGCCGGAGTTTGA+TGG - chr2.1:57873095-57873114 None:intergenic 45.0%
ATGCAACCTACAGGACCAAA+TGG + chr2.1:57875400-57875419 MS.gene00208:CDS 45.0%
CAAGAGCTCAAAGAAGAGGA+GGG + chr2.1:57874068-57874087 MS.gene00208:CDS 45.0%
CAAGGATCATGAGGCAAGTA+GGG - chr2.1:57873166-57873185 None:intergenic 45.0%
CACACTGATAAGTGGACATC+TGG - chr2.1:57874794-57874813 None:intergenic 45.0%
CCCAATCATTGGACTTCTGA+TGG - chr2.1:57875561-57875580 None:intergenic 45.0%
CTCTGATCAAGTCAGTCAAG+AGG + chr2.1:57875834-57875853 MS.gene00208:CDS 45.0%
CTTCAAACCCTTTCAGCTTC+TGG + chr2.1:57875046-57875065 MS.gene00208:CDS 45.0%
GATGTCCACTTATCAGTGTG+AGG + chr2.1:57874794-57874813 MS.gene00208:intron 45.0%
GGATAGAGGAAAAACCACGA+TGG - chr2.1:57875672-57875691 None:intergenic 45.0%
GTACGTTAGATGAGATACCG+TGG - chr2.1:57874277-57874296 None:intergenic 45.0%
GTACTGCTACTGGATATACC+CGG - chr2.1:57875460-57875479 None:intergenic 45.0%
GTGCATATGCCTGATATGGA+TGG + chr2.1:57873655-57873674 MS.gene00208:CDS 45.0%
GTTTCATGCGGAAGCTACTT+CGG + chr2.1:57875489-57875508 MS.gene00208:CDS 45.0%
TACCAAATGCAAGAGAGCTG+AGG + chr2.1:57873576-57873595 MS.gene00208:CDS 45.0%
TATAAGATCTCATGGGCCAC+TGG + chr2.1:57874730-57874749 MS.gene00208:intron 45.0%
TCAAGGATCATGAGGCAAGT+AGG - chr2.1:57873167-57873186 None:intergenic 45.0%
TGGTGAAGTTGAGGTTGACA+TGG - chr2.1:57873075-57873094 None:intergenic 45.0%
TGTCCACTTATCAGTGTGAG+GGG + chr2.1:57874796-57874815 MS.gene00208:intron 45.0%
TTGTCGAGTCTGTTGGATAG+AGG - chr2.1:57875686-57875705 None:intergenic 45.0%
TTTCATGCGGAAGCTACTTC+GGG + chr2.1:57875490-57875509 MS.gene00208:CDS 45.0%
TTTCCCTCTTAGATGCACTC+CGG + chr2.1:57875438-57875457 MS.gene00208:CDS 45.0%
! AGCGTATTTCTGAACCAGGT+TGG + chr2.1:57875865-57875884 MS.gene00208:intron 45.0%
! CAAAGAAGCCTCGAGTTGTT+TGG + chr2.1:57874133-57874152 MS.gene00208:CDS 45.0%
! CATGAGCGTATTTCTGAACC+AGG + chr2.1:57875861-57875880 MS.gene00208:CDS 45.0%
! TTAGTGATGTCGGCTGATGA+TGG + chr2.1:57873832-57873851 MS.gene00208:intron 45.0%
!! CTCCGTTTTTGCCTCTATGA+AGG + chr2.1:57873194-57873213 MS.gene00208:CDS 45.0%
!! GACTGACTTGATCAGAGAAG+GGG - chr2.1:57875831-57875850 None:intergenic 45.0%
!! GGAGTTTGATGGTGAAGTTG+AGG - chr2.1:57873084-57873103 None:intergenic 45.0%
!! GGCTGATGATGGAAAAAACG+TGG + chr2.1:57873843-57873862 MS.gene00208:CDS 45.0%
!!! TTTTTTTTTTTTTATTGATT+TGG + chr2.1:57873740-57873759 MS.gene00208:intron 5.0%
AGAACTGGCCAGAAGCTGAA+AGG - chr2.1:57875057-57875076 None:intergenic 50.0%
ATACCCGGAGTGCATCTAAG+AGG - chr2.1:57875445-57875464 None:intergenic 50.0%
ATGGCTCAGTCTCAACCAAG+AGG + chr2.1:57875322-57875341 MS.gene00208:CDS 50.0%
CACCAGTCTGCTCAATCCTT+TGG + chr2.1:57875241-57875260 MS.gene00208:CDS 50.0%
CCACGATGGCCTAAAATCGA+CGG - chr2.1:57875658-57875677 None:intergenic 50.0%
CCTTATCTCCGACCAGTTTC+CGG + chr2.1:57873109-57873128 MS.gene00208:CDS 50.0%
CGACGTGCATATGCCTGATA+TGG + chr2.1:57873651-57873670 MS.gene00208:CDS 50.0%
CTAGACTTTGCACCGAGAAC+TGG - chr2.1:57875072-57875091 None:intergenic 50.0%
GAACTGGCCAGAAGCTGAAA+GGG - chr2.1:57875056-57875075 None:intergenic 50.0%
GAGCCATCTGCATCAACCAA+GGG - chr2.1:57875309-57875328 None:intergenic 50.0%
GCAAGAGCTCAAAGAAGAGG+AGG + chr2.1:57874067-57874086 MS.gene00208:CDS 50.0%
GGCTATCAAGTCACCGCTAT+TGG + chr2.1:57874751-57874770 MS.gene00208:intron 50.0%
GTCCACTTATCAGTGTGAGG+GGG + chr2.1:57874797-57874816 MS.gene00208:intron 50.0%
TACCCGGAGTGCATCTAAGA+GGG - chr2.1:57875444-57875463 None:intergenic 50.0%
TCGCATATTTGCGCTGCCAA+AGG - chr2.1:57875260-57875279 None:intergenic 50.0%
TGACTCTGGCGTACTCATGA+AGG - chr2.1:57874993-57875012 None:intergenic 50.0%
TGAGCCATCTGCATCAACCA+AGG - chr2.1:57875310-57875329 None:intergenic 50.0%
TGCCAAAGGATTGAGCAGAC+TGG - chr2.1:57875246-57875265 None:intergenic 50.0%
TTCAGCTTCTGGCCAGTTCT+CGG + chr2.1:57875057-57875076 MS.gene00208:CDS 50.0%
TTCCCTCTTAGATGCACTCC+GGG + chr2.1:57875439-57875458 MS.gene00208:CDS 50.0%
! AAGCCCTTGGTTGATGCAGA+TGG + chr2.1:57875303-57875322 MS.gene00208:CDS 50.0%
! AAGTCCTGCTGCTTGAAGCT+TGG - chr2.1:57875095-57875114 None:intergenic 50.0%
! ACGTTTTCTCTAGTGAGCCC+AGG - chr2.1:57874521-57874540 None:intergenic 50.0%
! CACGCCTTTTGTACTGCTAC+TGG - chr2.1:57875470-57875489 None:intergenic 50.0%
! TTCAAAGCCTCGATTCGCAC+AGG - chr2.1:57873913-57873932 None:intergenic 50.0%
!!! GATCTCTCGCCGTCGATTTT+AGG + chr2.1:57875646-57875665 MS.gene00208:CDS 50.0%
CACCCCCTCACACTGATAAG+TGG - chr2.1:57874802-57874821 None:intergenic 55.0%
CCGGAAACTGGTCGGAGATA+AGG - chr2.1:57873112-57873131 None:intergenic 55.0%
CTAGCCAAGCTTCAAGCAGC+AGG + chr2.1:57875088-57875107 MS.gene00208:CDS 55.0%
CTGCTGCAAAGAGAAGCCCT+TGG + chr2.1:57875290-57875309 MS.gene00208:CDS 55.0%
CTGGTCGGAGATAAGGTTGC+CGG - chr2.1:57873105-57873124 None:intergenic 55.0%
TATCTCCGACCAGTTTCCGG+CGG + chr2.1:57873112-57873131 MS.gene00208:CDS 55.0%
! AGCGGTGACTTGATAGCCAG+TGG - chr2.1:57874749-57874768 None:intergenic 55.0%
! ATCTGGAGTGTGACTCGAGC+GGG - chr2.1:57874777-57874796 None:intergenic 55.0%
! CATCTGGAGTGTGACTCGAG+CGG - chr2.1:57874778-57874797 None:intergenic 55.0%
! TTCTCGCAGCACAAAGGCGT+TGG + chr2.1:57875977-57875996 MS.gene00208:intron 55.0%
!!! CCGTCGATTTTAGGCCATCG+TGG + chr2.1:57875655-57875674 MS.gene00208:CDS 55.0%
ACAAGTACACGGAGACCCGC+CGG - chr2.1:57873131-57873150 None:intergenic 60.0%
ATCTCCGACCAGTTTCCGGC+GGG + chr2.1:57873113-57873132 MS.gene00208:CDS 60.0%
TGACTCGAGCGGGCCAATAG+CGG - chr2.1:57874767-57874786 None:intergenic 60.0%
GAGACCCGCCGGAAACTGGT+CGG - chr2.1:57873120-57873139 None:intergenic 65.0%
CACGGAGACCCGCCGGAAAC+TGG - chr2.1:57873124-57873143 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 57873044 57876072 57873044 ID=MS.gene00208
chr2.1 mRNA 57873044 57876072 57873044 ID=MS.gene00208.t1;Parent=MS.gene00208
chr2.1 exon 57873044 57873215 57873044 ID=MS.gene00208.t1.exon1;Parent=MS.gene00208.t1
chr2.1 CDS 57873044 57873215 57873044 ID=cds.MS.gene00208.t1;Parent=MS.gene00208.t1
chr2.1 exon 57873575 57873727 57873575 ID=MS.gene00208.t1.exon2;Parent=MS.gene00208.t1
chr2.1 CDS 57873575 57873727 57873575 ID=cds.MS.gene00208.t1;Parent=MS.gene00208.t1
chr2.1 exon 57873836 57874210 57873836 ID=MS.gene00208.t1.exon3;Parent=MS.gene00208.t1
chr2.1 CDS 57873836 57874210 57873836 ID=cds.MS.gene00208.t1;Parent=MS.gene00208.t1
chr2.1 exon 57874480 57874556 57874480 ID=MS.gene00208.t1.exon4;Parent=MS.gene00208.t1
chr2.1 CDS 57874480 57874556 57874480 ID=cds.MS.gene00208.t1;Parent=MS.gene00208.t1
chr2.1 exon 57874926 57875882 57874926 ID=MS.gene00208.t1.exon5;Parent=MS.gene00208.t1
chr2.1 CDS 57874926 57875882 57874926 ID=cds.MS.gene00208.t1;Parent=MS.gene00208.t1
chr2.1 exon 57875986 57876072 57875986 ID=MS.gene00208.t1.exon6;Parent=MS.gene00208.t1
chr2.1 CDS 57875986 57876072 57875986 ID=cds.MS.gene00208.t1;Parent=MS.gene00208.t1
Gene Sequence

>MS.gene00208

ATGAAACTCAACAACAACAACAAAATTTCCATGTCAACCTCAACTTCACCATCAAACTCCGGCAACCTTATCTCCGACCAGTTTCCGGCGGGTCTCCGTGTACTTGTTGTTGATGATGACCCTACTTGCCTCATGATCCTTGAAAGAATGCTCCGTTTTTGCCTCTATGAAGGTCTTCAATCTTCATCTTTGTTTCACTATTGCCCATCATGAATTAGTGTTAAAAATCTTAACTTTACATCATTTTTTCAACGTTTATGTGCTTATAGATGCAAATTAGTAATACCCTTTTGTTTGATTGAGTTTTTTTATTCAGACCCATTTTTATTTTTAGTTCAGATTCTTGGTTGCAACCATCTTTCTTTTGAATTATGCTTTTGATATGATTTAATATGGTAGATTTGTTTGATAAGTGTTTTTTTCAACTATTGGAATTTATTCCAAAGTTAAAGTTTGAGACTTTAGTATATTGTGTAAGTTTTGTTGTTCACCATTTGATTCTGTTTTGCTTGTTGGGTATGTTTGTTTCAGTTACCAAATGCAAGAGAGCTGAGGTTGCATTATCACTTCTTAGAGAGAATAAAAACGGGTTCGACATTGTTTTAAGCGACGTGCATATGCCTGATATGGATGGATTCAAACTTTTGGAACATATTGGATTGGAGATGGATCTTCCTGTTATTAGTAAGCATTGTTTTTTTTTTTTTTTATTGATTTGGATCAGTTGATGCAATATGCAATGACATGAGTTGACATATTTTGTGTGTGCGTGTTTGTGTGAATGTGTTTTAGTGATGTCGGCTGATGATGGAAAAAACGTGGTAATGAAAGGTGTGACACACGGTGCTTGTGATTATCTAATCAAACCTGTGCGAATCGAGGCTTTGAAGAATATATGGCAGCATGTGGTTCGGAAGAGAAAGAATAACTGGAAAGATCTGAAGCAATCTGGTGGATTAGATGAAAGAGATCGAGAAAAAATTGTGTCCACTGATGCTAATTACTCATCTTCAACGAATGAAGGCAAGAGCTCAAAGAAGAGGAGGGATGAAGAAGATGAAGCAGATGATAGGGATGATTGTTCGACGTCAAAGAAGCCTCGAGTTGTTTGGTCTATTGAGCTTCATCAACAGTTTATGGCTGTTGTGAATCAATTGGGATTAGACAGTATGTGTCCCTTAATTATGTTATATCCTTGCAAAAATGTTCTCTTTCATCTTTGTTTGTTTTCCACGGTATCTCATCTAACGTACAAACCATTCCCTTAAAGTTTTTCCGAGATATAATCCTTGTGATTCTACAAGATTGTTTTTATCTCTAAATTAGCCCTTTTCCCCCATAGAATCTTAAATATTTCTGCTTGTCTAATTGATTTTTATATGTAATGTTAAGTAAAGTCGTCATCTTATGTTTTCAAAATTGATATTGATATGCAGAGGCTGTTCCTAAAAAGATTATGGAAATGATGAATGTTCCTGGGCTCACTAGAGAAAACGTTGCCAGTCATCTTCAGGTACGATATACTAAAAAGATTATACTTCCTCTATTTTTGTTATTGTTAAGAGTCCCACGTTAGATGAGATATGACCTATAATAAGTTAGTTTAGAAGTGGTGGACGATCCTCATCGTACAAACTGATTTTGTAAGGGTTGAGCAACTGATATTCTAGATGTTATTAGAGCCTATATAAGATCTCATGGGCCACTGGCTATCAAGTCACCGCTATTGGCCCGCTCGAGTCACACTCCAGATGTCCACTTATCAGTGTGAGGGGGTGTGTAAAGTCTAAAGAATCCACCTTGAATGTTAAGAGTTACAGTCATCAACGATATTGCTTGAAGAAGCTAACTTTCTTCATCTGTTTTTTATTTTCTTATACAGAAATACCGTTTGTATCTTCGAAGGTTGAGTGGAGTTTCCCAGCAGCAAAGTAACTTGAATAACTCCTTCATGAGTACGCCAGAGTCAATATTTGGAGGAACCTCCGTAAATGAAATTGATCTTCAAACCCTTTCAGCTTCTGGCCAGTTCTCGGTGCAAAGTCTAGCCAAGCTTCAAGCAGCAGGACTTTACAGTCCTGAGAACTCAAAACTAAGATTTGGAGATGGGCAACTGCAACATATGAGCAGCAGTAAACCGGTGAACTTTCTTCACGGAATCCCTATCAACATTGAGCCAAAGCAGTTTGCCAATTTTCACCAGTCTGCTCAATCCTTTGGCAGCGCAAATATGCGAGTCAATGCTTCTGCTGCAAAGAGAAGCCCTTGGTTGATGCAGATGGCTCAGTCTCAACCAAGAGGTCAGATGCTAAATGAGAATACAAATTCTCATGCTACAAGGTTTCCATCATCTTTAATGCAACCTACAGGACCAAATGGAATATCAGTCAATAGTTTCCCTCTTAGATGCACTCCGGGTATATCCAGTAGCAGTACAAAAGGCGTGTTTCATGCGGAAGCTACTTCGGGAATTAAAGGATTAGGGGGATTTAGTTATGATGTTTATAATGAACTCCATCAGAAGTCCAATGATTGGGGATTAGCAAATACAGGCCTGACATATGATGCTTCTCAACATGCAAATCCTTTACAAGGCAACATTGATCTCTCGCCGTCGATTTTAGGCCATCGTGGTTTTTCCTCTATCCAACAGACTCGACAAAATAGAGATGGTATGAGTCAAGGTGATCACCATAATGTTGGTCAACACCTCAATACAACTTTTGTCGACAATTCATTAAGGGGTAAGAGTGAAAGAATTCCGAGTACAAACATTGAGATCAACCCCTTCTCTGATCAAGTCAGTCAAGAGGATCTCATGAGCGTATTTCTGAACCAGGTTGGTTCTTCCTTTCTGTTGACCCTAAATATTTATGTTTCCTTACTTTTCCTTTATAGACAAATCATTTATTTTCATTTTTTTTTTTTTGTATTTCTCGCAGCACAAAGGCGTTGGACAAGTTGATAATGAGTTTGACTTTGATGGATATTCCGCAGACAACATTCCAGTTTATAACAGATCCTGTTAG
Protein sequence

>MS.gene00208.t1

MKLNNNNKISMSTSTSPSNSGNLISDQFPAGLRVLVVDDDPTCLMILERMLRFCLYEVTKCKRAEVALSLLRENKNGFDIVLSDVHMPDMDGFKLLEHIGLEMDLPVIMMSADDGKNVVMKGVTHGACDYLIKPVRIEALKNIWQHVVRKRKNNWKDLKQSGGLDERDREKIVSTDANYSSSTNEGKSSKKRRDEEDEADDRDDCSTSKKPRVVWSIELHQQFMAVVNQLGLDKAVPKKIMEMMNVPGLTRENVASHLQKYRLYLRRLSGVSQQQSNLNNSFMSTPESIFGGTSVNEIDLQTLSASGQFSVQSLAKLQAAGLYSPENSKLRFGDGQLQHMSSSKPVNFLHGIPINIEPKQFANFHQSAQSFGSANMRVNASAAKRSPWLMQMAQSQPRGQMLNENTNSHATRFPSSLMQPTGPNGISVNSFPLRCTPGISSSSTKGVFHAEATSGIKGLGGFSYDVYNELHQKSNDWGLANTGLTYDASQHANPLQGNIDLSPSILGHRGFSSIQQTRQNRDGMSQGDHHNVGQHLNTTFVDNSLRGKSERIPSTNIEINPFSDQVSQEDLMSVFLNQHKGVGQVDNEFDFDGYSADNIPVYNRSC