Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003751.t1 | AES89100.2 | 97.9 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 1.10E-126 | 462.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003751.t1 | Q8GUH2 | 39.3 | 196 | 100 | 6 | 36 | 221 | 42 | 228 | 2.0e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003751.t1 | G7JG61 | 97.9 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 7.8e-127 | 462.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene003751 | MS.gene019686 | PPI |
| MS.gene003751 | MS.gene000647 | PPI |
| MS.gene003751 | MS.gene92099 | PPI |
| MS.gene003751 | MS.gene056832 | PPI |
| MS.gene79923 | MS.gene003751 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003751.t1 | MTR_4g070020 | 97.881 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 1.00e-171 | 473 |
| MS.gene003751.t1 | MTR_4g070020 | 97.881 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 2.22e-170 | 471 |
| MS.gene003751.t1 | MTR_1g022190 | 49.754 | 203 | 86 | 4 | 26 | 224 | 55 | 245 | 9.18e-64 | 199 |
| MS.gene003751.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 30 | 220 | 3.81e-63 | 199 |
| MS.gene003751.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 55 | 245 | 7.04e-63 | 199 |
| MS.gene003751.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 60 | 250 | 1.05e-62 | 199 |
| MS.gene003751.t1 | MTR_8g028630 | 40.816 | 196 | 103 | 4 | 29 | 221 | 19 | 204 | 2.59e-38 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003751.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 1.04e-80 | 242 |
| MS.gene003751.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 6.79e-80 | 242 |
| MS.gene003751.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 6.79e-80 | 242 |
| MS.gene003751.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 6.79e-80 | 242 |
| MS.gene003751.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 1.72e-74 | 226 |
| MS.gene003751.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 1.19e-73 | 226 |
| MS.gene003751.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 1.19e-73 | 226 |
| MS.gene003751.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 2.25e-73 | 224 |
| MS.gene003751.t1 | AT1G01500 | 40.099 | 202 | 100 | 7 | 36 | 227 | 42 | 232 | 1.88e-36 | 131 |
Find 56 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGGCACAAAGTTCTTTA+AGG | 0.155540 | 4.1:+32766343 | MS.gene003751:CDS |
| GATACCCTTCTTGAAGTTAA+TGG | 0.212761 | 4.1:+32766068 | MS.gene003751:CDS |
| GAGAATGATGCAAATGAAAA+TGG | 0.217056 | 4.1:+32766518 | MS.gene003751:CDS |
| TCCTTCTGCGCTTGCTTTGC+AGG | 0.233001 | 4.1:+32766547 | MS.gene003751:CDS |
| GAGAATTCGTATAAGGTTAA+TGG | 0.283457 | 4.1:+32765867 | MS.gene003751:CDS |
| GGAAATGGACCAAGTGAAAA+TGG | 0.283915 | 4.1:+32765888 | MS.gene003751:CDS |
| ATTGCTGTTACATAATTCTA+AGG | 0.316159 | 4.1:-32766255 | None:intergenic |
| CTCATATCAACCCTCGATAA+AGG | 0.321091 | 4.1:+32765935 | MS.gene003751:CDS |
| AGAAGGGTATCAGGATTTAA+AGG | 0.331368 | 4.1:-32766056 | None:intergenic |
| GGAGAAGCAATTGTTGTTGC+CGG | 0.344534 | 4.1:+32766364 | MS.gene003751:CDS |
| AAAGATAAGCTTCTTCTGTT+TGG | 0.350138 | 4.1:+32766230 | MS.gene003751:CDS |
| TTAACTTCAAGAAGGGTATC+AGG | 0.353127 | 4.1:-32766065 | None:intergenic |
| TCGTATAAGGTTAATGGAAA+TGG | 0.363071 | 4.1:+32765873 | MS.gene003751:CDS |
| GTTAGAGAATCAAATTCTAA+TGG | 0.378979 | 4.1:+32766284 | MS.gene003751:CDS |
| TATATTGCCGGGTCCTTCTC+TGG | 0.379377 | 4.1:+32766413 | MS.gene003751:CDS |
| CGTTACCGTGGTTAGACATA+AGG | 0.381604 | 4.1:+32765961 | MS.gene003751:CDS |
| ACAATTGAGGTCTATATTGC+CGG | 0.393367 | 4.1:+32766401 | MS.gene003751:CDS |
| TTGTAAGCACTGATAGTATA+AGG | 0.394406 | 4.1:+32766174 | MS.gene003751:CDS |
| CGCGATGGAGAATTCGTATA+AGG | 0.406902 | 4.1:+32765860 | None:intergenic |
| TTCTTGAAGTTAATGGTGTT+CGG | 0.422504 | 4.1:+32766075 | MS.gene003751:CDS |
| TGATTAAGTGTGAGAATCTC+AGG | 0.428692 | 4.1:-32766029 | None:intergenic |
| CGGAGTAGTTTATATTCTGA+TGG | 0.435949 | 4.1:+32766095 | MS.gene003751:CDS |
| GCAATATAGACCTCAATTGT+TGG | 0.437528 | 4.1:-32766398 | None:intergenic |
| GGATTCCAACCCTTCTCAAA+AGG | 0.443968 | 4.1:+32766117 | MS.gene003751:CDS |
| TCCTTAAAGAACTTTGTGCC+TGG | 0.447833 | 4.1:-32766344 | None:intergenic |
| GGATAGGGTGGATAGAAAAT+CGG | 0.450168 | 4.1:+32766139 | MS.gene003751:CDS |
| TGTGAGTCGAATATAATTCC+AGG | 0.452189 | 4.1:+32766326 | MS.gene003751:CDS |
| TCATATCAACCCTCGATAAA+GGG | 0.470195 | 4.1:+32765936 | MS.gene003751:CDS |
| AGAATGATGCAAATGAAAAT+GGG | 0.470852 | 4.1:+32766519 | MS.gene003751:CDS |
| CAACCCTTCTCAAAAGGGAT+AGG | 0.480345 | 4.1:+32766123 | MS.gene003751:CDS |
| CAAATTCTAATGGACAAAGT+TGG | 0.492533 | 4.1:+32766294 | MS.gene003751:CDS |
| GTTACCGTGGTTAGACATAA+GGG | 0.505953 | 4.1:+32765962 | MS.gene003751:CDS |
| GATTCCAACCCTTCTCAAAA+GGG | 0.526688 | 4.1:+32766118 | MS.gene003751:CDS |
| GGAGTAGTTTATATTCTGAT+GGG | 0.528712 | 4.1:+32766096 | MS.gene003751:CDS |
| ACCTGCAAAGCAAGCGCAGA+AGG | 0.534939 | 4.1:-32766548 | None:intergenic |
| AACCCTTCTCAAAAGGGATA+GGG | 0.548703 | 4.1:+32766124 | MS.gene003751:CDS |
| GAGTGGTAGTGTGAAGTTTG+AGG | 0.549063 | 4.1:+32766199 | MS.gene003751:CDS |
| CTCTACTCTTCCAACAATTG+AGG | 0.556408 | 4.1:+32766388 | MS.gene003751:CDS |
| GAACACCATTAACTTCAAGA+AGG | 0.569037 | 4.1:-32766073 | None:intergenic |
| CAATTGAGGTCTATATTGCC+GGG | 0.582339 | 4.1:+32766402 | MS.gene003751:CDS |
| TCTGGAATTGCATTCAACGC+CGG | 0.588676 | 4.1:-32766497 | None:intergenic |
| AAAATGGATACTCTATTGCT+AGG | 0.589815 | 4.1:+32765904 | MS.gene003751:CDS |
| CGATAAAGGGTTCGTTACCG+TGG | 0.592561 | 4.1:+32765949 | MS.gene003751:CDS |
| AAGGTCTTCGTTAAGATGAT+CGG | 0.592566 | 4.1:-32766440 | None:intergenic |
| CGGAGTGCCAGAGAAGGACC+CGG | 0.592882 | 4.1:-32766420 | None:intergenic |
| GATGCAAATGAAAATGGGAA+AGG | 0.596141 | 4.1:+32766524 | MS.gene003751:CDS |
| AACACCATTAACTTCAAGAA+GGG | 0.596194 | 4.1:-32766072 | None:intergenic |
| CCTTCTCAAAAGGGATAGGG+TGG | 0.600087 | 4.1:+32766127 | MS.gene003751:CDS |
| ATTGTTGGAAGAGTAGAGTC+CGG | 0.603903 | 4.1:-32766383 | None:intergenic |
| CGGTAACGAACCCTTTATCG+AGG | 0.628590 | 4.1:-32765946 | None:intergenic |
| ACTGATAGTATAAGGATGAG+TGG | 0.634943 | 4.1:+32766182 | MS.gene003751:CDS |
| GATGATCGGAGTGCCAGAGA+AGG | 0.637274 | 4.1:-32766426 | None:intergenic |
| GGTAACGAACCCTTTATCGA+GGG | 0.642976 | 4.1:-32765945 | None:intergenic |
| AACACCCTTATGTCTAACCA+CGG | 0.647619 | 4.1:-32765966 | None:intergenic |
| TTAGAATTATGTAACAGCAA+TGG | 0.648523 | 4.1:+32766257 | MS.gene003751:CDS |
| AAGTGTGAGAATCTCAGGAG+TGG | 0.662685 | 4.1:-32766024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAATGATGCAAATGAAAAT+GGG | + | chr4.1:32766519-32766538 | MS.gene003751:CDS | 25.0% |
| ! | GTTAGAGAATCAAATTCTAA+TGG | + | chr4.1:32766284-32766303 | MS.gene003751:CDS | 25.0% |
| ! | TTAGAATTATGTAACAGCAA+TGG | + | chr4.1:32766257-32766276 | MS.gene003751:CDS | 25.0% |
| !! | ATTGCTGTTACATAATTCTA+AGG | - | chr4.1:32766258-32766277 | None:intergenic | 25.0% |
| AAAATGGATACTCTATTGCT+AGG | + | chr4.1:32765904-32765923 | MS.gene003751:CDS | 30.0% | |
| AAAGATAAGCTTCTTCTGTT+TGG | + | chr4.1:32766230-32766249 | MS.gene003751:CDS | 30.0% | |
| AACACCATTAACTTCAAGAA+GGG | - | chr4.1:32766075-32766094 | None:intergenic | 30.0% | |
| GAGAATGATGCAAATGAAAA+TGG | + | chr4.1:32766518-32766537 | MS.gene003751:CDS | 30.0% | |
| GAGAATTCGTATAAGGTTAA+TGG | + | chr4.1:32765867-32765886 | MS.gene003751:CDS | 30.0% | |
| GGAGTAGTTTATATTCTGAT+GGG | + | chr4.1:32766096-32766115 | MS.gene003751:CDS | 30.0% | |
| GTTCTCAAAAAAGACATACA+AGG | + | chr4.1:32766471-32766490 | MS.gene003751:CDS | 30.0% | |
| TCGTATAAGGTTAATGGAAA+TGG | + | chr4.1:32765873-32765892 | MS.gene003751:CDS | 30.0% | |
| TTCTTGAAGTTAATGGTGTT+CGG | + | chr4.1:32766075-32766094 | MS.gene003751:CDS | 30.0% | |
| ! | CAAATTCTAATGGACAAAGT+TGG | + | chr4.1:32766294-32766313 | MS.gene003751:CDS | 30.0% |
| ! | TTTTCATTTGCATCATTCTC+TGG | - | chr4.1:32766518-32766537 | None:intergenic | 30.0% |
| !! | ATAGAGTATCCATTTTCACT+TGG | - | chr4.1:32765900-32765919 | None:intergenic | 30.0% |
| !! | TTGTAAGCACTGATAGTATA+AGG | + | chr4.1:32766174-32766193 | MS.gene003751:CDS | 30.0% |
| !! | TTTTTGAGAACTAAGATGCA+AGG | - | chr4.1:32766462-32766481 | None:intergenic | 30.0% |
| !!! | ACATAAGGGTGTTTTATGTA+AGG | + | chr4.1:32765976-32765995 | MS.gene003751:CDS | 30.0% |
| !!! | CATAAGGGTGTTTTATGTAA+GGG | + | chr4.1:32765977-32765996 | MS.gene003751:CDS | 30.0% |
| AAAAAGACATACAAGGAAGC+CGG | + | chr4.1:32766478-32766497 | MS.gene003751:CDS | 35.0% | |
| AAGGTCTTCGTTAAGATGAT+CGG | - | chr4.1:32766443-32766462 | None:intergenic | 35.0% | |
| ACAATTGAGGTCTATATTGC+CGG | + | chr4.1:32766401-32766420 | MS.gene003751:CDS | 35.0% | |
| ACTGATAGTATAAGGATGAG+TGG | + | chr4.1:32766182-32766201 | MS.gene003751:CDS | 35.0% | |
| CGGAGTAGTTTATATTCTGA+TGG | + | chr4.1:32766095-32766114 | MS.gene003751:CDS | 35.0% | |
| GAACACCATTAACTTCAAGA+AGG | - | chr4.1:32766076-32766095 | None:intergenic | 35.0% | |
| GATACCCTTCTTGAAGTTAA+TGG | + | chr4.1:32766068-32766087 | MS.gene003751:CDS | 35.0% | |
| GATGCAAATGAAAATGGGAA+AGG | + | chr4.1:32766524-32766543 | MS.gene003751:CDS | 35.0% | |
| GCAATATAGACCTCAATTGT+TGG | - | chr4.1:32766401-32766420 | None:intergenic | 35.0% | |
| TCATATCAACCCTCGATAAA+GGG | + | chr4.1:32765936-32765955 | MS.gene003751:CDS | 35.0% | |
| TGATTAAGTGTGAGAATCTC+AGG | - | chr4.1:32766032-32766051 | None:intergenic | 35.0% | |
| TGTGAGTCGAATATAATTCC+AGG | + | chr4.1:32766326-32766345 | MS.gene003751:CDS | 35.0% | |
| !! | AGAAGGGTATCAGGATTTAA+AGG | - | chr4.1:32766059-32766078 | None:intergenic | 35.0% |
| !! | TTAACTTCAAGAAGGGTATC+AGG | - | chr4.1:32766068-32766087 | None:intergenic | 35.0% |
| AACACCCTTATGTCTAACCA+CGG | - | chr4.1:32765969-32765988 | None:intergenic | 40.0% | |
| AACCCTTCTCAAAAGGGATA+GGG | + | chr4.1:32766124-32766143 | MS.gene003751:CDS | 40.0% | |
| ATTGTTGGAAGAGTAGAGTC+CGG | - | chr4.1:32766386-32766405 | None:intergenic | 40.0% | |
| CAATTGAGGTCTATATTGCC+GGG | + | chr4.1:32766402-32766421 | MS.gene003751:CDS | 40.0% | |
| CTCATATCAACCCTCGATAA+AGG | + | chr4.1:32765935-32765954 | MS.gene003751:CDS | 40.0% | |
| CTCTACTCTTCCAACAATTG+AGG | + | chr4.1:32766388-32766407 | MS.gene003751:CDS | 40.0% | |
| GATTCCAACCCTTCTCAAAA+GGG | + | chr4.1:32766118-32766137 | MS.gene003751:CDS | 40.0% | |
| GGAAATGGACCAAGTGAAAA+TGG | + | chr4.1:32765888-32765907 | MS.gene003751:CDS | 40.0% | |
| GGATAGGGTGGATAGAAAAT+CGG | + | chr4.1:32766139-32766158 | MS.gene003751:CDS | 40.0% | |
| GTTACCGTGGTTAGACATAA+GGG | + | chr4.1:32765962-32765981 | MS.gene003751:CDS | 40.0% | |
| TCCAGGCACAAAGTTCTTTA+AGG | + | chr4.1:32766343-32766362 | MS.gene003751:CDS | 40.0% | |
| TCCTTAAAGAACTTTGTGCC+TGG | - | chr4.1:32766347-32766366 | None:intergenic | 40.0% | |
| AAGTGTGAGAATCTCAGGAG+TGG | - | chr4.1:32766027-32766046 | None:intergenic | 45.0% | |
| CAACCCTTCTCAAAAGGGAT+AGG | + | chr4.1:32766123-32766142 | MS.gene003751:CDS | 45.0% | |
| CGTTACCGTGGTTAGACATA+AGG | + | chr4.1:32765961-32765980 | MS.gene003751:CDS | 45.0% | |
| GGATTCCAACCCTTCTCAAA+AGG | + | chr4.1:32766117-32766136 | MS.gene003751:CDS | 45.0% | |
| GGTAACGAACCCTTTATCGA+GGG | - | chr4.1:32765948-32765967 | None:intergenic | 45.0% | |
| ! | GAGTGGTAGTGTGAAGTTTG+AGG | + | chr4.1:32766199-32766218 | MS.gene003751:CDS | 45.0% |
| ! | GGAGAAGCAATTGTTGTTGC+CGG | + | chr4.1:32766364-32766383 | MS.gene003751:CDS | 45.0% |
| ! | TCTGGAATTGCATTCAACGC+CGG | - | chr4.1:32766500-32766519 | None:intergenic | 45.0% |
| !! | CACCCTATCCCTTTTGAGAA+GGG | - | chr4.1:32766129-32766148 | None:intergenic | 45.0% |
| !! | CTATCCCTTTTGAGAAGGGT+TGG | - | chr4.1:32766125-32766144 | None:intergenic | 45.0% |
| CCTTCTCAAAAGGGATAGGG+TGG | + | chr4.1:32766127-32766146 | MS.gene003751:CDS | 50.0% | |
| CGATAAAGGGTTCGTTACCG+TGG | + | chr4.1:32765949-32765968 | MS.gene003751:CDS | 50.0% | |
| CGGTAACGAACCCTTTATCG+AGG | - | chr4.1:32765949-32765968 | None:intergenic | 50.0% | |
| TATATTGCCGGGTCCTTCTC+TGG | + | chr4.1:32766413-32766432 | MS.gene003751:CDS | 50.0% | |
| !! | CCACCCTATCCCTTTTGAGA+AGG | - | chr4.1:32766130-32766149 | None:intergenic | 50.0% |
| ! | GATGATCGGAGTGCCAGAGA+AGG | - | chr4.1:32766429-32766448 | None:intergenic | 55.0% |
| ! | CGGAGTGCCAGAGAAGGACC+CGG | - | chr4.1:32766423-32766442 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 32765864 | 32766568 | 32765864 | ID=MS.gene003751 |
| chr4.1 | mRNA | 32765864 | 32766568 | 32765864 | ID=MS.gene003751.t1;Parent=MS.gene003751 |
| chr4.1 | exon | 32765864 | 32766568 | 32765864 | ID=MS.gene003751.t1.exon1;Parent=MS.gene003751.t1 |
| chr4.1 | CDS | 32765864 | 32766568 | 32765864 | ID=cds.MS.gene003751.t1;Parent=MS.gene003751.t1 |
| Gene Sequence |
| Protein sequence |
