Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000647.t1 | XP_024629527.1 | 98.4 | 183 | 3 | 0 | 3 | 185 | 46 | 228 | 2.40E-98 | 368.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000647.t1 | Q8GUH2 | 53.4 | 191 | 78 | 4 | 5 | 186 | 64 | 252 | 5.1e-49 | 195.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000647.t1 | A0A072TZ44 | 98.4 | 183 | 3 | 0 | 3 | 185 | 46 | 228 | 1.7e-98 | 368.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene50027 | MS.gene000647 | PPI |
| MS.gene45677 | MS.gene000647 | PPI |
| MS.gene056706 | MS.gene000647 | PPI |
| MS.gene003757 | MS.gene000647 | PPI |
| MS.gene003751 | MS.gene000647 | PPI |
| MS.gene23256 | MS.gene000647 | PPI |
| MS.gene23257 | MS.gene000647 | PPI |
| MS.gene003753 | MS.gene000647 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000647.t1 | MTR_8g028630 | 98.361 | 183 | 3 | 0 | 3 | 185 | 46 | 228 | 7.18e-130 | 369 |
| MS.gene000647.t1 | MTR_4g070020 | 39.286 | 168 | 90 | 3 | 3 | 161 | 57 | 221 | 7.46e-30 | 110 |
| MS.gene000647.t1 | MTR_4g070020 | 39.286 | 168 | 90 | 3 | 3 | 161 | 57 | 221 | 7.36e-29 | 109 |
| MS.gene000647.t1 | MTR_1g022190 | 39.412 | 170 | 95 | 5 | 18 | 186 | 104 | 266 | 2.39e-28 | 107 |
| MS.gene000647.t1 | MTR_1g022190 | 41.379 | 145 | 78 | 4 | 18 | 161 | 79 | 217 | 7.39e-27 | 104 |
| MS.gene000647.t1 | MTR_1g022190 | 41.379 | 145 | 78 | 4 | 18 | 161 | 104 | 242 | 8.55e-27 | 104 |
| MS.gene000647.t1 | MTR_1g022190 | 41.379 | 145 | 78 | 4 | 18 | 161 | 109 | 247 | 1.71e-26 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000647.t1 | AT1G01500 | 53.403 | 191 | 78 | 4 | 5 | 186 | 64 | 252 | 2.93e-63 | 199 |
| MS.gene000647.t1 | AT1G19400 | 41.237 | 194 | 93 | 6 | 3 | 187 | 54 | 235 | 2.74e-34 | 122 |
| MS.gene000647.t1 | AT1G19400 | 42.262 | 168 | 84 | 3 | 3 | 161 | 54 | 217 | 2.89e-33 | 121 |
| MS.gene000647.t1 | AT1G19400 | 42.262 | 168 | 84 | 3 | 3 | 161 | 54 | 217 | 2.89e-33 | 121 |
| MS.gene000647.t1 | AT1G19400 | 42.262 | 168 | 84 | 3 | 3 | 161 | 54 | 217 | 2.89e-33 | 121 |
| MS.gene000647.t1 | AT1G75180 | 39.181 | 171 | 88 | 3 | 3 | 161 | 58 | 224 | 2.17e-31 | 114 |
| MS.gene000647.t1 | AT1G75180 | 36.923 | 195 | 102 | 4 | 3 | 180 | 58 | 248 | 2.61e-31 | 115 |
| MS.gene000647.t1 | AT1G75180 | 39.181 | 171 | 88 | 3 | 3 | 161 | 58 | 224 | 1.45e-30 | 114 |
| MS.gene000647.t1 | AT1G75180 | 39.181 | 171 | 88 | 3 | 3 | 161 | 58 | 224 | 1.45e-30 | 114 |
Find 0 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGAATAAAATGCTTCTT+AGG | - | 57397:943-962 | None:intergenic | 20.0% |
| GTGACATAGTGTTAAACATT+CGG | - | 57397:374-393 | None:intergenic | 30.0% | |
| ! | GGTTTTTCTCTTGTAATCAA+CGG | + | 57397:409-428 | MS.gene000647:CDS | 30.0% |
| !! | AAAAAAGTACCTGCAATTTG+GGG | - | 57397:909-928 | None:intergenic | 30.0% |
| !! | AAAAACCGATTTCACGTTTT+GGG | - | 57397:396-415 | None:intergenic | 30.0% |
| !! | CAAAAAAGTACCTGCAATTT+GGG | - | 57397:910-929 | None:intergenic | 30.0% |
| AGATGAGGAAATGCATATGA+TGG | + | 57397:843-862 | MS.gene000647:CDS | 35.0% | |
| ATATGCATTTCCTCATCTTC+GGG | - | 57397:841-860 | None:intergenic | 35.0% | |
| CACATTATCAGTGCTAACAT+AGG | - | 57397:515-534 | None:intergenic | 35.0% | |
| CATATGCATTTCCTCATCTT+CGG | - | 57397:842-861 | None:intergenic | 35.0% | |
| GATCATGTTAGTGCTATGAA+TGG | + | 57397:871-890 | MS.gene000647:CDS | 35.0% | |
| TTGAGGTGTATGAGAAAGAT+GGG | + | 57397:557-576 | MS.gene000647:CDS | 35.0% | |
| ! | ACTGATAATGTGAGAATCAC+AGG | + | 57397:523-542 | MS.gene000647:CDS | 35.0% |
| ! | ATAGTCTTGCTCAAAATCAC+AGG | - | 57397:766-785 | None:intergenic | 35.0% |
| ! | TTTGAGGTGTATGAGAAAGA+TGG | + | 57397:556-575 | MS.gene000647:CDS | 35.0% |
| !! | AAAGATGGGTTGTTTCTATG+TGG | + | 57397:571-590 | MS.gene000647:CDS | 35.0% |
| !! | AAGATGGGTTGTTTCTATGT+GGG | + | 57397:572-591 | MS.gene000647:CDS | 35.0% |
| !! | AGTTCCTAGACATGCTATTT+TGG | + | 57397:807-826 | MS.gene000647:CDS | 35.0% |
| !! | CCAAAAAAGTACCTGCAATT+TGG | - | 57397:911-930 | None:intergenic | 35.0% |
| !! | GAAAAACCGATTTCACGTTT+TGG | - | 57397:397-416 | None:intergenic | 35.0% |
| !! | GAACGTTTGGATGTTGATTT+GGG | + | 57397:601-620 | MS.gene000647:CDS | 35.0% |
| !! | TGGATGTTGATTTGGGAAAT+GGG | + | 57397:608-627 | MS.gene000647:CDS | 35.0% |
| !! | TTGGATGTTGATTTGGGAAA+TGG | + | 57397:607-626 | MS.gene000647:CDS | 35.0% |
| !!! | CCAAATTGCAGGTACTTTTT+TGG | + | 57397:908-927 | MS.gene000647:CDS | 35.0% |
| AGACTATTTCGTTGAGTCCT+AGG | + | 57397:779-798 | MS.gene000647:CDS | 40.0% | |
| ATCAATTGGATCGGGTTCTT+CGG | + | 57397:666-685 | MS.gene000647:CDS | 40.0% | |
| GATAATGTGAGAATCACAGG+TGG | + | 57397:526-545 | MS.gene000647:CDS | 40.0% | |
| GCAACATAAACTTCAACCGA+TGG | - | 57397:724-743 | None:intergenic | 40.0% | |
| GCATCCAAAATAGCATGTCT+AGG | - | 57397:814-833 | None:intergenic | 40.0% | |
| TATGTTGCTGGATGTTGTTC+TGG | + | 57397:736-755 | MS.gene000647:CDS | 40.0% | |
| TCGGTTGAAGTTTATGTTGC+TGG | + | 57397:724-743 | MS.gene000647:CDS | 40.0% | |
| TGTCACCCAAAACGTGAAAT+CGG | + | 57397:388-407 | MS.gene000647:CDS | 40.0% | |
| ! | GGAAATGCATATGATGGAGA+AGG | + | 57397:849-868 | MS.gene000647:CDS | 40.0% |
| ! | GGAACGTTTGGATGTTGATT+TGG | + | 57397:600-619 | MS.gene000647:CDS | 40.0% |
| ! | TTGGGATATGGATTGTCATG+TGG | + | 57397:636-655 | MS.gene000647:CDS | 40.0% |
| !! | GTTGTTTCTATGTGGGTCAT+TGG | + | 57397:579-598 | MS.gene000647:CDS | 40.0% |
| AAATGGGTCTGGTTGGGATA+TGG | + | 57397:624-643 | MS.gene000647:CDS | 45.0% | |
| AATTCCACACCCCAAATTGC+AGG | + | 57397:897-916 | MS.gene000647:CDS | 45.0% | |
| ACTCTTTATCGACACGGTCA+CGG | - | 57397:480-499 | None:intergenic | 45.0% | |
| ATGTGGGTCATTGGAACGTT+TGG | + | 57397:588-607 | MS.gene000647:CDS | 45.0% | |
| ATTTGGGAAATGGGTCTGGT+TGG | + | 57397:617-636 | MS.gene000647:CDS | 45.0% | |
| CAGCAGACTCTTTATCGACA+CGG | - | 57397:486-505 | None:intergenic | 45.0% | |
| CTATTTCGTTGAGTCCTAGG+AGG | + | 57397:782-801 | MS.gene000647:CDS | 45.0% | |
| TGTCGATAAAGAGTCTGCTG+AGG | + | 57397:486-505 | MS.gene000647:CDS | 45.0% | |
| TTTGGGAAATGGGTCTGGTT+GGG | + | 57397:618-637 | MS.gene000647:CDS | 45.0% | |
| ! | GGATATGGATTGTCATGTGG+CGG | + | 57397:639-658 | MS.gene000647:CDS | 45.0% |
| !! | GTTGATTTGGGAAATGGGTC+TGG | + | 57397:613-632 | MS.gene000647:CDS | 45.0% |
| !!! | CGGGTTCTTCGGCTTTTTTT+AGG | + | 57397:677-696 | MS.gene000647:CDS | 45.0% |
| !!! | GCTTTTTTTAGGCCGAAGCT+CGG | + | 57397:688-707 | MS.gene000647:CDS | 45.0% |
| CATGTGGCGGTTGGATCAAT+TGG | + | 57397:652-671 | MS.gene000647:CDS | 50.0% | |
| GCGGTTGGATCAATTGGATC+GGG | + | 57397:658-677 | MS.gene000647:CDS | 50.0% | |
| GGATGCTATTCCCGAAGATG+AGG | + | 57397:828-847 | MS.gene000647:CDS | 50.0% | |
| GGCGGTTGGATCAATTGGAT+CGG | + | 57397:657-676 | MS.gene000647:CDS | 50.0% | |
| TGTCTAGGAACTCTCCTCCT+AGG | - | 57397:799-818 | None:intergenic | 50.0% | |
| ! | AGTACCTGCAATTTGGGGTG+TGG | - | 57397:904-923 | None:intergenic | 50.0% |
| ! | ATGGATTGTCATGTGGCGGT+TGG | + | 57397:643-662 | MS.gene000647:CDS | 50.0% |
| ! | CACAGGTGGTGCTGAATTTG+AGG | + | 57397:540-559 | MS.gene000647:CDS | 50.0% |
| CTTTATCGACACGGTCACGG+CGG | - | 57397:477-496 | None:intergenic | 55.0% | |
| !! | TAGGCCGAAGCTCGGTGTTT+CGG | + | 57397:696-715 | MS.gene000647:CDS | 55.0% |
| GGAGCCGAAACACCGAGCTT+CGG | - | 57397:703-722 | None:intergenic | 60.0% | |
| !! | GCTCGGTGTTTCGGCTCCAT+CGG | + | 57397:705-724 | MS.gene000647:CDS | 60.0% |
| ! | GCGGAAGAGGGTGCAGGGAT+CGG | - | 57397:439-458 | None:intergenic | 65.0% |
| GGAAGCGGTGGGGCGGAAGA+GGG | - | 57397:451-470 | None:intergenic | 70.0% | |
| TCGACACGGTCACGGCGGAG+CGG | - | 57397:472-491 | None:intergenic | 70.0% | |
| ! | GTGGGGCGGAAGAGGGTGCA+GGG | - | 57397:444-463 | None:intergenic | 70.0% |
| ACGGCGGAGCGGAAGCGGTG+GGG | - | 57397:461-480 | None:intergenic | 75.0% | |
| CACGGCGGAGCGGAAGCGGT+GGG | - | 57397:462-481 | None:intergenic | 75.0% | |
| CGGAAGCGGTGGGGCGGAAG+AGG | - | 57397:452-471 | None:intergenic | 75.0% | |
| CGGTCACGGCGGAGCGGAAG+CGG | - | 57397:466-485 | None:intergenic | 75.0% | |
| TCACGGCGGAGCGGAAGCGG+TGG | - | 57397:463-482 | None:intergenic | 75.0% | |
| ! | GGTGGGGCGGAAGAGGGTGC+AGG | - | 57397:445-464 | None:intergenic | 75.0% |
| ! | GCGGAGCGGAAGCGGTGGGG+CGG | - | 57397:458-477 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57397 | gene | 367 | 969 | 367 | ID=MS.gene000647 |
| 57397 | mRNA | 367 | 969 | 367 | ID=MS.gene000647.t1;Parent=MS.gene000647 |
| 57397 | exon | 367 | 969 | 367 | ID=MS.gene000647.t1.exon1;Parent=MS.gene000647.t1 |
| 57397 | CDS | 367 | 969 | 367 | ID=cds.MS.gene000647.t1;Parent=MS.gene000647.t1 |
| Gene Sequence |
| Protein sequence |
