AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003757


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003757.t1 MTR_4g070020 99.000 200 1 1 1 199 112 311 1.23e-145 407
MS.gene003757.t1 MTR_4g070020 98.400 125 1 1 1 124 112 236 5.11e-84 249
MS.gene003757.t1 MTR_1g022190 44.175 206 96 6 1 199 119 312 2.46e-50 165
MS.gene003757.t1 MTR_1g022190 44.175 206 96 6 1 199 144 337 4.43e-50 165
MS.gene003757.t1 MTR_1g022190 44.175 206 96 6 1 199 149 342 6.14e-50 165
MS.gene003757.t1 MTR_8g028630 41.315 213 99 7 1 198 101 302 5.41e-42 144
MS.gene003757.t1 MTR_1g022190 41.026 117 56 3 1 116 144 248 1.39e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003757.t1 AT1G75180 55.172 203 87 3 1 199 113 315 3.59e-69 213
MS.gene003757.t1 AT1G75180 55.172 203 87 3 1 199 113 315 3.59e-69 213
MS.gene003757.t1 AT1G19400 52.970 202 85 5 1 199 109 303 2.39e-61 193
MS.gene003757.t1 AT1G19400 52.970 202 85 5 1 199 109 303 2.39e-61 193
MS.gene003757.t1 AT1G19400 52.970 202 85 5 1 199 109 303 2.39e-61 193
MS.gene003757.t1 AT1G01500 39.545 220 104 8 1 199 116 327 1.78e-39 137
MS.gene003757.t1 AT1G75180 53.279 122 55 1 1 120 113 234 5.89e-35 124
MS.gene003757.t1 AT1G75180 53.279 122 55 1 1 120 113 234 2.47e-34 123
MS.gene003757.t1 AT1G19400 51.562 128 56 3 1 125 109 233 9.49e-33 118

Find 53 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCACAAAGTTCTTTA+AGG 0.155540 4.2:+36914594 MS.gene003757:CDS
GAGAATGATGCAAATGAAAA+TGG 0.217056 4.2:+36914769 MS.gene003757:CDS
TCCTTCTGCGCTTGCTTTGC+AGG 0.233001 4.2:+36914798 MS.gene003757:CDS
CATGGTTTAACGCCGGTGTT+AGG 0.255714 4.2:+36915619 MS.gene003757:CDS
GTTAGGGTTGGTGTTGGTAT+TGG 0.297925 4.2:+36915636 MS.gene003757:CDS
ATTGCTGTTACATAATTCTA+AGG 0.316159 4.2:-36914506 None:intergenic
GGAGAAGCAATTGTTGTTGC+CGG 0.344534 4.2:+36914615 MS.gene003757:CDS
AAAGATAAGCTTCTTCTGTT+TGG 0.350138 4.2:+36914481 MS.gene003757:CDS
GCGAAGATGGGGAACTTTCA+TGG 0.371984 4.2:+36915601 MS.gene003757:CDS
AGAATAGCCGTCTTCTGAAT+TGG 0.372025 4.2:-36915728 None:intergenic
GTTAGAGAATCAAATTCTAA+TGG 0.378979 4.2:+36914535 MS.gene003757:CDS
TATATTGCCGGGTCCTTCTC+TGG 0.379377 4.2:+36914664 MS.gene003757:CDS
ACAATTGAGGTCTATATTGC+CGG 0.393367 4.2:+36914652 MS.gene003757:CDS
TGTTTGTCATACATGTAATC+TGG 0.402010 4.2:-36915528 None:intergenic
CTTAGTGTTTGTCTTGGAAT+CGG 0.423405 4.2:+36915660 MS.gene003757:CDS
GCAATATAGACCTCAATTGT+TGG 0.437528 4.2:-36914649 None:intergenic
CTTGGAATCGGCATTGGAGT+TGG 0.441010 4.2:+36915672 MS.gene003757:CDS
TCCTTAAAGAACTTTGTGCC+TGG 0.447833 4.2:-36914595 None:intergenic
GTTTGTCTTGGAATCGGCAT+TGG 0.451016 4.2:+36915666 MS.gene003757:CDS
TGTGAGTCGAATATAATTCC+AGG 0.452189 4.2:+36914577 MS.gene003757:CDS
ATGGTTTAACGCCGGTGTTA+GGG 0.458989 4.2:+36915620 MS.gene003757:CDS
AGAATGATGCAAATGAAAAT+GGG 0.470852 4.2:+36914770 MS.gene003757:CDS
CGTATGCTGTTCTTGTGTAT+AGG 0.473133 4.2:-36915572 None:intergenic
TGAATTGGCCGGTGGCACCT+TGG 0.475731 4.2:-36915713 None:intergenic
ATTGGACTTAGTGTTTGTCT+TGG 0.481547 4.2:+36915654 MS.gene003757:CDS
GCATACGCTGACGGCGAAGA+TGG 0.492307 4.2:+36915588 MS.gene003757:CDS
CAAATTCTAATGGACAAAGT+TGG 0.492533 4.2:+36914545 MS.gene003757:CDS
CATACGCTGACGGCGAAGAT+GGG 0.511131 4.2:+36915589 MS.gene003757:CDS
ACCTGCAAAGCAAGCGCAGA+AGG 0.534939 4.2:-36914799 None:intergenic
GAGTGGTAGTGTGAAGTTTG+AGG 0.549063 4.2:+36914450 MS.gene003757:CDS
GCCGGTGTTAGGGTTGGTGT+TGG 0.549235 4.2:+36915630 MS.gene003757:CDS
CTCTACTCTTCCAACAATTG+AGG 0.556408 4.2:+36914639 MS.gene003757:CDS
AAATCATACCAAGGTGCCAC+CGG 0.568352 4.2:+36915705 MS.gene003757:CDS
TTTAACGCCGGTGTTAGGGT+TGG 0.570434 4.2:+36915624 MS.gene003757:CDS
TAGCCGTCTTCTGAATTGGC+CGG 0.581948 4.2:-36915724 None:intergenic
CAATTGAGGTCTATATTGCC+GGG 0.582339 4.2:+36914653 MS.gene003757:CDS
CCACCGGCCAATTCAGAAGA+CGG 0.588469 4.2:+36915721 MS.gene003757:CDS
TCTGGAATTGCATTCAACGC+CGG 0.588676 4.2:-36914748 None:intergenic
AAGGTCTTCGTTAAGATGAT+CGG 0.592566 4.2:-36914691 None:intergenic
CGGAGTGCCAGAGAAGGACC+CGG 0.592882 4.2:-36914671 None:intergenic
GATGCAAATGAAAATGGGAA+AGG 0.596141 4.2:+36914775 MS.gene003757:CDS
ATTGTTGGAAGAGTAGAGTC+CGG 0.603903 4.2:-36914634 None:intergenic
ACAAGAACAGCATACGCTGA+CGG 0.606360 4.2:+36915579 MS.gene003757:CDS
ACCAACACCAACCCTAACAC+CGG 0.618909 4.2:-36915631 None:intergenic
ATACGCTGACGGCGAAGATG+GGG 0.629010 4.2:+36915590 MS.gene003757:CDS
ACTGATAGTATAAGGATGAG+TGG 0.634943 4.2:+36914433 None:intergenic
CATGATGATGAGGATTATCA+CGG 0.635768 4.2:+36915549 MS.gene003757:CDS
GATGATCGGAGTGCCAGAGA+AGG 0.637274 4.2:-36914677 None:intergenic
CCGTCTTCTGAATTGGCCGG+TGG 0.642624 4.2:-36915721 None:intergenic
TTAGAATTATGTAACAGCAA+TGG 0.648523 4.2:+36914508 MS.gene003757:CDS
GAACTTTCATGGTTTAACGC+CGG 0.650464 4.2:+36915612 MS.gene003757:CDS
GTATGACAAACATGATGATG+AGG 0.669080 4.2:+36915539 MS.gene003757:CDS
ATACTCGTTAAATCATACCA+AGG 0.711189 4.2:+36915696 MS.gene003757:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCATTTACATAAAATAATA+AGG - chr4.2:36915501-36915520 None:intergenic 15.0%
!!! AAATTTTTAAAATCTTGTCA+AGG - chr4.2:36915411-36915430 None:intergenic 15.0%
!! ATTTAAATAAGCACATGAAA+AGG - chr4.2:36914847-36914866 None:intergenic 20.0%
!! GATATAACTGTAAATTATCT+TGG + chr4.2:36915242-36915261 MS.gene003757:intron 20.0%
!!! AGGAATTATCTATAATTTGT+TGG - chr4.2:36915481-36915500 None:intergenic 20.0%
!!! GGAATTATCTATAATTTGTT+GGG - chr4.2:36915480-36915499 None:intergenic 20.0%
! AGAATGATGCAAATGAAAAT+GGG + chr4.2:36914770-36914789 MS.gene003757:CDS 25.0%
! ATATTTATGACCAAACCAAA+TGG + chr4.2:36915431-36915450 MS.gene003757:intron 25.0%
! GTTAGAGAATCAAATTCTAA+TGG + chr4.2:36914535-36914554 MS.gene003757:CDS 25.0%
! TTAGAATTATGTAACAGCAA+TGG + chr4.2:36914508-36914527 MS.gene003757:CDS 25.0%
! TTCTTCATAAGATTTCCATT+TGG - chr4.2:36915449-36915468 None:intergenic 25.0%
!! ATTGCTGTTACATAATTCTA+AGG - chr4.2:36914509-36914528 None:intergenic 25.0%
!! TACAAAGTGTTCTTAAATAG+TGG + chr4.2:36915017-36915036 MS.gene003757:intron 25.0%
!!! TCTATAATTTGTTGGGAAAA+TGG - chr4.2:36915473-36915492 None:intergenic 25.0%
!!! TTATTTTATGTAAATGCTGC+AGG + chr4.2:36915503-36915522 MS.gene003757:intron 25.0%
AAAGATAAGCTTCTTCTGTT+TGG + chr4.2:36914481-36914500 MS.gene003757:CDS 30.0%
ATACTCGTTAAATCATACCA+AGG + chr4.2:36915696-36915715 MS.gene003757:CDS 30.0%
ATTCAAGCAACAAGTTAACA+AGG - chr4.2:36914899-36914918 None:intergenic 30.0%
CATAAGATTTCCATTTGGTT+TGG - chr4.2:36915444-36915463 None:intergenic 30.0%
GAGAATGATGCAAATGAAAA+TGG + chr4.2:36914769-36914788 MS.gene003757:CDS 30.0%
GTTCTCAAAAAAGACATACA+AGG + chr4.2:36914722-36914741 MS.gene003757:CDS 30.0%
TGTTTGTCATACATGTAATC+TGG - chr4.2:36915531-36915550 None:intergenic 30.0%
! CAAATTCTAATGGACAAAGT+TGG + chr4.2:36914545-36914564 MS.gene003757:CDS 30.0%
! GATTTGTTCAAATCCCTAAA+GGG - chr4.2:36914970-36914989 None:intergenic 30.0%
! TTTTCATTTGCATCATTCTC+TGG - chr4.2:36914769-36914788 None:intergenic 30.0%
!! ATTGGCTCAACGTAATTTTA+TGG + chr4.2:36915127-36915146 MS.gene003757:intron 30.0%
!! TTTTTGAGAACTAAGATGCA+AGG - chr4.2:36914713-36914732 None:intergenic 30.0%
AAAAAGACATACAAGGAAGC+CGG + chr4.2:36914729-36914748 MS.gene003757:CDS 35.0%
AAGGTCTTCGTTAAGATGAT+CGG - chr4.2:36914694-36914713 None:intergenic 35.0%
ACAATTGAGGTCTATATTGC+CGG + chr4.2:36914652-36914671 MS.gene003757:CDS 35.0%
ATTATGTTCCATCTCCTACT+AGG - chr4.2:36915214-36915233 None:intergenic 35.0%
ATTGGACTTAGTGTTTGTCT+TGG + chr4.2:36915654-36915673 MS.gene003757:CDS 35.0%
CTTAGTGTTTGTCTTGGAAT+CGG + chr4.2:36915660-36915679 MS.gene003757:CDS 35.0%
GATGCAAATGAAAATGGGAA+AGG + chr4.2:36914775-36914794 MS.gene003757:CDS 35.0%
GCAATATAGACCTCAATTGT+TGG - chr4.2:36914652-36914671 None:intergenic 35.0%
TGATATGCGATTGACAATAC+AGG + chr4.2:36915089-36915108 MS.gene003757:intron 35.0%
TGTGAGTCGAATATAATTCC+AGG + chr4.2:36914577-36914596 MS.gene003757:CDS 35.0%
TTTGAGAAATAGCTATCCTC+CGG + chr4.2:36915321-36915340 MS.gene003757:intron 35.0%
! AACTATTTTCATCACTCGTC+AGG - chr4.2:36915387-36915406 None:intergenic 35.0%
! ATGCACTGACATAGTTAAAG+AGG - chr4.2:36914873-36914892 None:intergenic 35.0%
! CATGATGATGAGGATTATCA+CGG + chr4.2:36915549-36915568 MS.gene003757:CDS 35.0%
! CGATTTGTTCAAATCCCTAA+AGG - chr4.2:36914971-36914990 None:intergenic 35.0%
! GTATGACAAACATGATGATG+AGG + chr4.2:36915539-36915558 MS.gene003757:CDS 35.0%
!! ATTATCTTGGCTCTTTTCTG+AGG + chr4.2:36915255-36915274 MS.gene003757:intron 35.0%
AATAGTGTATCGCAGAACAG+TGG - chr4.2:36914994-36915013 None:intergenic 40.0%
AGAATAGCCGTCTTCTGAAT+TGG - chr4.2:36915731-36915750 None:intergenic 40.0%
ATTGTTGGAAGAGTAGAGTC+CGG - chr4.2:36914637-36914656 None:intergenic 40.0%
CAATTGAGGTCTATATTGCC+GGG + chr4.2:36914653-36914672 MS.gene003757:CDS 40.0%
CGTATGCTGTTCTTGTGTAT+AGG - chr4.2:36915575-36915594 None:intergenic 40.0%
CTCTACTCTTCCAACAATTG+AGG + chr4.2:36914639-36914658 MS.gene003757:CDS 40.0%
GAACTTTCATGGTTTAACGC+CGG + chr4.2:36915612-36915631 MS.gene003757:CDS 40.0%
GCTATGGCATTGTACTTTAG+TGG + chr4.2:36915041-36915060 MS.gene003757:intron 40.0%
GGAGTCGTTTGAATATGACA+TGG + chr4.2:36915148-36915167 MS.gene003757:intron 40.0%
GTTCTTAAATAGTGGCGCTA+TGG + chr4.2:36915025-36915044 MS.gene003757:intron 40.0%
TCCAGGCACAAAGTTCTTTA+AGG + chr4.2:36914594-36914613 MS.gene003757:CDS 40.0%
TCCTTAAAGAACTTTGTGCC+TGG - chr4.2:36914598-36914617 None:intergenic 40.0%
! AGGTCTAGCGTTGCTTTAAT+TGG + chr4.2:36915109-36915128 MS.gene003757:intron 40.0%
ACAAGAACAGCATACGCTGA+CGG + chr4.2:36915579-36915598 MS.gene003757:CDS 45.0%
ACTATAGCGCTATGCCCTTT+AGG + chr4.2:36914953-36914972 MS.gene003757:intron 45.0%
AGTGCCGCAATTTGACAACA+CGG - chr4.2:36914936-36914955 None:intergenic 45.0%
CTATAGCGCTATGCCCTTTA+GGG + chr4.2:36914954-36914973 MS.gene003757:intron 45.0%
GACTCCGTGTTGTCAAATTG+CGG + chr4.2:36914929-36914948 MS.gene003757:intron 45.0%
GACTGATGTCACAACCTAGT+AGG + chr4.2:36915197-36915216 MS.gene003757:intron 45.0%
GTTTGTCTTGGAATCGGCAT+TGG + chr4.2:36915666-36915685 MS.gene003757:CDS 45.0%
TGTCACAACCTAGTAGGAGA+TGG + chr4.2:36915203-36915222 MS.gene003757:intron 45.0%
! AAATCATACCAAGGTGCCAC+CGG + chr4.2:36915705-36915724 MS.gene003757:CDS 45.0%
! ATGGTTTAACGCCGGTGTTA+GGG + chr4.2:36915620-36915639 MS.gene003757:CDS 45.0%
! GAGTGGTAGTGTGAAGTTTG+AGG + chr4.2:36914450-36914469 MS.gene003757:CDS 45.0%
! GGAGAAGCAATTGTTGTTGC+CGG + chr4.2:36914615-36914634 MS.gene003757:CDS 45.0%
! TCTGGAATTGCATTCAACGC+CGG - chr4.2:36914751-36914770 None:intergenic 45.0%
!! GTTAGGGTTGGTGTTGGTAT+TGG + chr4.2:36915636-36915655 MS.gene003757:CDS 45.0%
ACCAACACCAACCCTAACAC+CGG - chr4.2:36915634-36915653 None:intergenic 50.0%
CATGGTTTAACGCCGGTGTT+AGG + chr4.2:36915619-36915638 MS.gene003757:CDS 50.0%
GCGAAGATGGGGAACTTTCA+TGG + chr4.2:36915601-36915620 MS.gene003757:CDS 50.0%
TAGCCGTCTTCTGAATTGGC+CGG - chr4.2:36915727-36915746 None:intergenic 50.0%
TATATTGCCGGGTCCTTCTC+TGG + chr4.2:36914664-36914683 MS.gene003757:CDS 50.0%
TGACAACACGGAGTCACACA+TGG - chr4.2:36914924-36914943 None:intergenic 50.0%
! CTTGGAATCGGCATTGGAGT+TGG + chr4.2:36915672-36915691 MS.gene003757:CDS 50.0%
! TTTAACGCCGGTGTTAGGGT+TGG + chr4.2:36915624-36915643 MS.gene003757:CDS 50.0%
ACCTGCAAAGCAAGCGCAGA+AGG - chr4.2:36914802-36914821 None:intergenic 55.0%
ATACGCTGACGGCGAAGATG+GGG + chr4.2:36915590-36915609 MS.gene003757:CDS 55.0%
CATACGCTGACGGCGAAGAT+GGG + chr4.2:36915589-36915608 MS.gene003757:CDS 55.0%
CCACCGGCCAATTCAGAAGA+CGG + chr4.2:36915721-36915740 MS.gene003757:CDS 55.0%
TCCTTCTGCGCTTGCTTTGC+AGG + chr4.2:36914798-36914817 MS.gene003757:CDS 55.0%
! GATGATCGGAGTGCCAGAGA+AGG - chr4.2:36914680-36914699 None:intergenic 55.0%
CCGTCTTCTGAATTGGCCGG+TGG - chr4.2:36915724-36915743 None:intergenic 60.0%
GCATACGCTGACGGCGAAGA+TGG + chr4.2:36915588-36915607 MS.gene003757:CDS 60.0%
TGAATTGGCCGGTGGCACCT+TGG - chr4.2:36915716-36915735 None:intergenic 60.0%
!! GCCGGTGTTAGGGTTGGTGT+TGG + chr4.2:36915630-36915649 MS.gene003757:CDS 60.0%
GCCTGCATGTGCTCTCGAGC+CGG - chr4.2:36915343-36915362 None:intergenic 65.0%
TGCATGTGCTCTCGAGCCGG+AGG - chr4.2:36915340-36915359 None:intergenic 65.0%
! CGGAGTGCCAGAGAAGGACC+CGG - chr4.2:36914674-36914693 None:intergenic 65.0%
! TCCGGCTCGAGAGCACATGC+AGG + chr4.2:36915339-36915358 MS.gene003757:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 36914448 36915752 36914448 ID=MS.gene003757
chr4.2 mRNA 36914448 36915752 36914448 ID=MS.gene003757.t1;Parent=MS.gene003757
chr4.2 exon 36914448 36914819 36914448 ID=MS.gene003757.t1.exon1;Parent=MS.gene003757.t1
chr4.2 CDS 36914448 36914819 36914448 ID=cds.MS.gene003757.t1;Parent=MS.gene003757.t1
chr4.2 exon 36915525 36915752 36915525 ID=MS.gene003757.t1.exon2;Parent=MS.gene003757.t1
chr4.2 CDS 36915525 36915752 36915525 ID=cds.MS.gene003757.t1;Parent=MS.gene003757.t1
Gene Sequence

>MS.gene003757

ATGAGTGGTAGTGTGAAGTTTGAGGTGTTTGATAAAGATAAGCTTCTTCTGTTTGGTACCTTAGAATTATGTAACAGCAATGGTGTTGTTAGAGAATCAAATTCTAATGGACAAAGTTGGAGCATGAAGTGTGAGTCGAATATAATTCCAGGCACAAAGTTCTTTAAGGAGAAGCAATTGTTGTTGCCGGACTCTACTCTTCCAACAATTGAGGTCTATATTGCCGGGTCCTTCTCTGGCACTCCGATCATCTTAACGAAGACCTTGCATCTTAGTTCTCAAAAAAGACATACAAGGAAGCCGGCGTTGAATGCAATTCCAGAGAATGATGCAAATGAAAATGGGAAAGGTCCTTCTGCGCTTGCTTTGCAGGTAATTAGTGCTCGTTGAATCATGCCTTTTCATGTGCTTATTTAAATTATCCTCTTTAACTATGTCAGTGCATCATCCTTGTTAACTTGTTGCTTGAATTTCCATGTGTGACTCCGTGTTGTCAAATTGCGGCACTATAGCGCTATGCCCTTTAGGGATTTGAACAAATCGCCACTGTTCTGCGATACACTATTCAATACAAAGTGTTCTTAAATAGTGGCGCTATGGCATTGTACTTTAGTGGAACTTGATCAAACTGTTATTTTCTGTGATATGCGATTGACAATACAGGTCTAGCGTTGCTTTAATTGGCTCAACGTAATTTTATGGAGTCGTTTGAATATGACATGGTTTGACTGATATGAGCATTTGTATCTGACTGATGTCACAACCTAGTAGGAGATGGAACATAATAAAACTATGATATAACTGTAAATTATCTTGGCTCTTTTCTGAGGTTTCATTTTCTACTTAACTTTTCTTGCTCAATTCTGTATTGTGTTTGAGAAATAGCTATCCTCCGGCTCGAGAGCACATGCAGGCTGCAAATTAACGTCACACACTCCTGACGAGTGATGAAAATAGTTTCCTTGACAAGATTTTAAAAATTTATATTTATGACCAAACCAAATGGAAATCTTATGAAGAATCCATTTTCCCAACAAATTATAGATAATTCCTTATTATTTTATGTAAATGCTGCAGGCTCCAGATTACATGTATGACAAACATGATGATGAGGATTATCACGGCCTATACACAAGAACAGCATACGCTGACGGCGAAGATGGGGAACTTTCATGGTTTAACGCCGGTGTTAGGGTTGGTGTTGGTATTGGACTTAGTGTTTGTCTTGGAATCGGCATTGGAGTTGGCATACTCGTTAAATCATACCAAGGTGCCACCGGCCAATTCAGAAGACGGCTATTCTAA

Protein sequence

>MS.gene003757.t1

MSGSVKFEVFDKDKLLLFGTLELCNSNGVVRESNSNGQSWSMKCESNIIPGTKFFKEKQLLLPDSTLPTIEVYIAGSFSGTPIILTKTLHLSSQKRHTRKPALNAIPENDANENGKGPSALALQAPDYMYDKHDDEDYHGLYTRTAYADGEDGELSWFNAGVRVGVGIGLSVCLGIGIGVGILVKSYQGATGQFRRRLF