AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004004

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004004.t1 MTR_4g064730 90.549 328 13 2 1 326 1 312 0.0 588
MS.gene004004.t1 MTR_7g089010 43.534 232 76 5 8 223 5 197 4.63e-43 150
MS.gene004004.t1 MTR_4g121020 66.176 68 23 0 126 193 106 173 4.14e-25 105
MS.gene004004.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 4.87e-25 105
MS.gene004004.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.25e-25 105
MS.gene004004.t1 MTR_2g034960 49.057 106 47 1 126 224 206 311 3.62e-24 103
MS.gene004004.t1 MTR_7g117705 46.281 121 63 2 130 248 190 310 3.60e-23 99.8
MS.gene004004.t1 MTR_3g106220 49.485 97 46 1 130 226 197 290 3.73e-23 100
MS.gene004004.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.65e-22 98.6
MS.gene004004.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.79e-22 98.6
MS.gene004004.t1 MTR_4g131570 68.852 61 19 0 128 188 200 260 1.60e-21 95.5
MS.gene004004.t1 MTR_1g032570 57.143 77 29 1 113 189 117 189 2.12e-21 95.1
MS.gene004004.t1 MTR_1g032570 57.143 77 29 1 113 189 186 258 2.84e-21 94.7
MS.gene004004.t1 MTR_1g013170 44.954 109 45 2 130 227 190 294 7.92e-21 93.2
MS.gene004004.t1 MTR_4g131580 68.852 61 19 0 128 188 197 257 1.48e-20 92.8
MS.gene004004.t1 MTR_8g079940 48.571 105 46 3 97 200 180 277 7.25e-20 90.5
MS.gene004004.t1 MTR_0450s0040 39.200 125 67 2 128 246 166 287 1.99e-19 87.8
MS.gene004004.t1 MTR_3g102600 58.333 60 25 0 130 189 82 141 3.59e-19 84.0
MS.gene004004.t1 MTR_1g013180 51.316 76 37 0 130 205 341 416 3.85e-19 88.6
MS.gene004004.t1 MTR_3g102600 58.333 60 25 0 130 189 131 190 4.87e-19 84.7
MS.gene004004.t1 MTR_7g026400 53.125 64 30 0 124 187 150 213 1.39e-18 86.7
MS.gene004004.t1 MTR_4g131600 57.812 64 27 0 126 189 196 259 2.18e-18 86.3
MS.gene004004.t1 MTR_3g086100 35.526 152 87 4 100 247 103 247 8.31e-18 84.3
MS.gene004004.t1 MTR_3g086100 35.526 152 87 4 100 247 174 318 9.29e-18 84.3
MS.gene004004.t1 MTR_4g021760 32.768 177 101 5 95 258 131 302 3.74e-17 80.9
MS.gene004004.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 3.91e-16 79.3
MS.gene004004.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 4.03e-16 79.3
MS.gene004004.t1 MTR_3g102590 51.389 72 33 1 130 199 142 213 4.05e-16 77.0
MS.gene004004.t1 MTR_4g098870 37.500 120 64 1 76 184 283 402 4.29e-16 79.3
MS.gene004004.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 4.94e-16 79.3
MS.gene004004.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 4.96e-16 79.3
MS.gene004004.t1 MTR_5g014040 53.333 60 28 0 130 189 310 369 2.57e-15 77.0
MS.gene004004.t1 MTR_2g090675 43.038 79 43 1 130 206 160 238 2.16e-14 73.9
MS.gene004004.t1 MTR_4g086835 49.206 63 32 0 127 189 270 332 3.31e-14 73.2
MS.gene004004.t1 MTR_4g078810 44.928 69 38 0 130 198 157 225 3.52e-14 73.2
MS.gene004004.t1 MTR_4g021790 38.532 109 55 1 80 188 129 225 6.62e-14 71.2
MS.gene004004.t1 MTR_5g041350 38.679 106 53 4 128 227 12 111 2.60e-13 70.1
MS.gene004004.t1 MTR_5g014040 49.231 65 28 1 130 189 270 334 2.60e-13 70.9
MS.gene004004.t1 MTR_5g014040 49.231 65 28 1 130 189 310 374 2.87e-13 70.9
MS.gene004004.t1 MTR_3g102600 50.943 53 26 0 130 182 82 134 2.94e-13 66.6
MS.gene004004.t1 MTR_3g102600 50.943 53 26 0 130 182 131 183 3.63e-13 67.4
MS.gene004004.t1 MTR_5g017980 48.333 60 31 0 130 189 191 250 4.76e-13 69.3
MS.gene004004.t1 MTR_5g017980 48.333 60 31 0 130 189 192 251 4.80e-13 69.3
MS.gene004004.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 4.94e-13 69.3
MS.gene004004.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 5.63e-13 69.3
MS.gene004004.t1 MTR_4g086835 48.438 64 32 1 127 189 270 333 6.81e-13 69.3
MS.gene004004.t1 MTR_1g090670 60.000 55 21 1 131 184 44 98 7.01e-13 68.6
MS.gene004004.t1 MTR_4g113140 40.404 99 52 3 119 210 218 316 7.52e-13 69.3
MS.gene004004.t1 MTR_7g069660 42.202 109 47 3 82 183 25 124 9.07e-13 68.6
MS.gene004004.t1 MTR_8g105600 56.140 57 25 0 134 190 190 246 9.28e-13 69.3
MS.gene004004.t1 MTR_2g027800 52.055 73 29 2 131 197 39 111 9.90e-13 68.2
MS.gene004004.t1 MTR_2g027800 60.000 55 21 1 131 184 39 93 1.27e-12 67.8
MS.gene004004.t1 MTR_2g086450 55.172 58 25 1 128 184 42 99 1.52e-12 68.2
MS.gene004004.t1 MTR_1g093080 43.243 74 41 1 127 199 207 280 2.00e-12 67.8
MS.gene004004.t1 MTR_4g021855 40.625 96 55 2 95 188 130 225 2.08e-12 67.0
MS.gene004004.t1 MTR_1g080330 50.685 73 34 2 112 183 157 228 2.93e-12 66.6
MS.gene004004.t1 MTR_1g080330 49.315 73 35 2 112 183 157 228 3.16e-12 67.0
MS.gene004004.t1 MTR_6g032990 47.222 72 36 2 128 198 39 109 3.20e-12 67.4
MS.gene004004.t1 MTR_7g098250 39.080 87 52 1 98 183 229 315 3.41e-12 67.4
MS.gene004004.t1 MTR_6g045327 39.326 89 52 1 101 187 140 228 4.43e-12 66.6
MS.gene004004.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 6.11e-12 65.9
MS.gene004004.t1 MTR_2g027860 48.611 72 35 2 131 201 49 119 6.56e-12 65.9
MS.gene004004.t1 MTR_5g027440 54.545 55 24 1 131 184 25 79 6.88e-12 65.5
MS.gene004004.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 9.57e-12 65.5
MS.gene004004.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.04e-11 65.9
MS.gene004004.t1 MTR_5g017980 47.541 61 31 1 130 189 192 252 1.44e-11 64.7
MS.gene004004.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.55e-11 65.1
MS.gene004004.t1 MTR_5g054300 42.105 76 43 1 130 204 197 272 1.67e-11 64.7
MS.gene004004.t1 MTR_1g053830 50.943 53 25 1 133 184 20 72 1.95e-11 60.5
MS.gene004004.t1 MTR_6g453220 63.830 47 17 0 148 194 380 426 3.51e-11 64.3
MS.gene004004.t1 MTR_0223s0040 49.231 65 32 1 120 183 7 71 3.79e-11 63.2
MS.gene004004.t1 MTR_1g112370 50.794 63 30 1 122 183 13 75 5.17e-11 63.2
MS.gene004004.t1 MTR_3g102590 54.902 51 23 0 130 180 142 192 5.25e-11 61.6
MS.gene004004.t1 MTR_4g081710 50.000 58 28 1 128 184 14 71 5.40e-11 63.2
MS.gene004004.t1 MTR_2g023580 48.148 81 39 3 105 183 29 108 6.75e-11 62.0
MS.gene004004.t1 MTR_2g084230 53.125 64 29 1 121 183 87 150 9.15e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004004.t1 AT3G46640 56.596 235 76 4 1 215 1 229 3.58e-77 239
MS.gene004004.t1 AT3G46640 56.596 235 76 4 1 215 1 229 3.58e-77 239
MS.gene004004.t1 AT3G46640 56.596 235 76 4 1 215 1 229 2.08e-76 237
MS.gene004004.t1 AT5G59570 53.414 249 101 7 11 250 25 267 6.90e-73 227
MS.gene004004.t1 AT5G59570 53.414 249 101 7 11 250 25 267 6.90e-73 227
MS.gene004004.t1 AT3G10760 47.596 208 80 5 8 210 5 188 2.79e-39 141
MS.gene004004.t1 AT5G05090 47.196 214 56 6 1 210 1 161 2.31e-38 137
MS.gene004004.t1 AT2G40970 72.340 94 17 1 126 210 100 193 3.95e-38 136
MS.gene004004.t1 AT3G16857 52.128 94 40 1 100 193 141 229 1.60e-24 104
MS.gene004004.t1 AT3G16857 52.128 94 40 1 100 193 211 299 1.99e-24 104
MS.gene004004.t1 AT3G16857 52.128 94 40 1 100 193 211 299 2.27e-24 103
MS.gene004004.t1 AT2G25180 48.182 110 48 1 94 194 149 258 2.28e-22 98.2
MS.gene004004.t1 AT2G01760 57.895 76 29 1 130 202 134 209 7.87e-22 94.4
MS.gene004004.t1 AT5G58080 55.696 79 30 1 128 201 191 269 1.01e-21 96.3
MS.gene004004.t1 AT2G01760 57.895 76 29 1 130 202 199 274 2.20e-21 94.0
MS.gene004004.t1 AT2G01760 57.895 76 29 1 130 202 227 302 3.92e-21 93.6
MS.gene004004.t1 AT4G31920 44.792 96 50 1 130 225 182 274 1.21e-18 87.0
MS.gene004004.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.10e-16 79.3
MS.gene004004.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.11e-16 79.7
MS.gene004004.t1 AT2G20570 32.911 158 100 2 41 196 65 218 6.53e-16 78.6
MS.gene004004.t1 AT5G07210 42.268 97 50 2 98 192 193 285 7.05e-16 79.0
MS.gene004004.t1 AT5G07210 46.053 76 39 1 119 192 218 293 7.44e-16 78.6
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.40e-15 77.0
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.40e-15 77.0
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.40e-15 77.0
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.40e-15 77.0
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.40e-15 77.0
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.40e-15 77.0
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 3.64e-15 76.6
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 3.64e-15 76.6
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 3.64e-15 76.6
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 3.64e-15 76.6
MS.gene004004.t1 AT4G18020 29.680 219 120 5 84 293 242 435 3.64e-15 76.6
MS.gene004004.t1 AT2G20570 36.885 122 68 2 80 196 117 234 4.12e-15 76.3
MS.gene004004.t1 AT1G67710 43.478 92 48 2 100 190 100 188 1.07e-14 75.1
MS.gene004004.t1 AT1G67710 43.478 92 48 2 100 190 91 179 1.18e-14 74.7
MS.gene004004.t1 AT3G12730 52.055 73 33 2 113 184 6 77 1.68e-14 72.4
MS.gene004004.t1 AT1G67710 43.478 92 48 2 100 190 165 253 1.83e-14 74.3
MS.gene004004.t1 AT2G27070 45.679 81 41 2 130 207 224 304 2.47e-14 73.9
MS.gene004004.t1 AT3G04030 49.275 69 34 1 128 196 42 109 7.62e-14 72.0
MS.gene004004.t1 AT1G79430 53.623 69 30 2 117 184 21 88 9.38e-14 71.6
MS.gene004004.t1 AT5G45580 56.364 55 23 1 131 184 7 61 3.78e-13 68.9
MS.gene004004.t1 AT3G04030 50.000 70 33 2 128 196 42 110 4.37e-13 69.7
MS.gene004004.t1 AT5G45580 56.364 55 23 1 131 184 23 77 4.53e-13 68.9
MS.gene004004.t1 AT3G04030 50.000 70 33 2 128 196 42 110 5.18e-13 69.7
MS.gene004004.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.19e-13 68.6
MS.gene004004.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.33e-13 69.3
MS.gene004004.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.33e-13 69.3
MS.gene004004.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.36e-13 68.6
MS.gene004004.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.59e-13 69.3
MS.gene004004.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.14e-13 69.3
MS.gene004004.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.14e-13 69.3
MS.gene004004.t1 AT4G13640 58.182 55 22 1 131 184 37 91 7.42e-13 68.6
MS.gene004004.t1 AT4G13640 58.182 55 22 1 131 184 37 91 7.92e-13 68.2
MS.gene004004.t1 AT1G13300 42.529 87 46 3 130 212 180 266 9.01e-13 68.6
MS.gene004004.t1 AT3G62670 55.932 59 21 2 130 183 210 268 9.03e-13 68.9
MS.gene004004.t1 AT2G03500 47.692 65 33 1 125 188 227 291 1.13e-12 68.9
MS.gene004004.t1 AT2G01060 45.055 91 45 3 118 203 2 92 1.76e-12 67.4
MS.gene004004.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.15e-11 65.9
MS.gene004004.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.15e-11 65.9
MS.gene004004.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.15e-11 65.9
MS.gene004004.t1 AT2G38300 40.196 102 57 2 107 207 30 128 1.28e-11 65.1
MS.gene004004.t1 AT1G49560 44.118 68 37 1 122 188 183 250 2.22e-11 64.3
MS.gene004004.t1 AT1G25550 48.333 60 30 1 130 188 208 267 3.46e-11 63.9
MS.gene004004.t1 AT5G49240 50.980 51 25 0 130 180 222 272 4.38e-11 63.2
MS.gene004004.t1 AT3G25790 40.449 89 49 3 135 219 200 288 7.28e-11 63.2

Find 91 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGATGCATCATATTAAA+TGG 0.267208 4.3:+39342104 None:intergenic
GGAGTGAGATCGTGGAGGTT+AGG 0.288087 4.3:+39342944 None:intergenic
CATTTAATATGATGCATCAT+AGG 0.291545 4.3:-39342103 MS.gene004004:CDS
GATGAGTTTGCCTCCTCCTT+CGG 0.309025 4.3:-39342153 MS.gene004004:CDS
GAGTGAGATCGTGGAGGTTA+GGG 0.316953 4.3:+39342945 None:intergenic
ACATCATCCACATGGGCATA+TGG 0.344716 4.3:-39342177 MS.gene004004:CDS
GTGGATGTTGTTGCTCATCT+TGG 0.347586 4.3:-39342569 MS.gene004004:CDS
ATGAGTTTGCCTCCTCCTTC+GGG 0.352579 4.3:-39342152 MS.gene004004:CDS
TTCAACTTCTTCTACTTCGC+TGG 0.386278 4.3:+39342760 None:intergenic
CTCGAAGGTGGTGGTGGATA+AGG 0.394424 4.3:+39342233 None:intergenic
GAACAACGGAGACGATAAAA+AGG 0.405625 4.3:-39342631 MS.gene004004:CDS
CATCATCCACATGGGCATAT+GGG 0.413116 4.3:-39342176 MS.gene004004:CDS
TCAAATGGAGGGCATGGAAA+TGG 0.414953 4.3:-39342311 MS.gene004004:CDS
AAGAGAGTACGGAGAGGCTC+CGG 0.421773 4.3:+39342869 None:intergenic
TCAAATGCTAATCATTCAAA+TGG 0.429533 4.3:-39342326 MS.gene004004:CDS
TGATTGTTCCGGAGTTGCTT+CGG 0.434817 4.3:+39342807 None:intergenic
TGAGGATGAGAATGTGGATT+CGG 0.435504 4.3:+39342071 None:intergenic
TGGATGTTGTTGCTCATCTT+GGG 0.442275 4.3:-39342568 MS.gene004004:CDS
TTCTTCTACTTCGCTGGACA+CGG 0.442290 4.3:+39342766 None:intergenic
CCGCAGAGTTTGCATGAAAC+CGG 0.443486 4.3:-39342374 MS.gene004004:CDS
TTCCATGGCTTTGATCCCAC+CGG 0.445349 4.3:-39342918 MS.gene004004:CDS
AGTGGAAGCTGAAGCAGATT+CGG 0.449529 4.3:-39342660 MS.gene004004:CDS
ACGTGTAACGGAGTGGGAAA+AGG 0.453107 4.3:-39342972 MS.gene004004:CDS
AGAGAGTACGGAGAGGCTCC+GGG 0.454039 4.3:+39342870 None:intergenic
GAACCCGAGCGAGATGGATC+AGG 0.456693 4.3:-39342728 MS.gene004004:CDS
CTGAAAGCGGAAGCGAGCTC+CGG 0.460721 4.3:+39342899 None:intergenic
TGATGTCCCATACCAATCAT+AGG 0.460751 4.3:+39342194 None:intergenic
TCATGCAAACTCTGCGGCAC+AGG 0.465750 4.3:+39342380 None:intergenic
GATTTGATGAGTGATTGTTC+CGG 0.471479 4.3:+39342796 None:intergenic
ACAGCTACATAAAAGATTTG+TGG 0.472539 4.3:-39342588 MS.gene004004:CDS
CGATCTCACTCCTCTTTCCA+TGG 0.482732 4.3:-39342933 MS.gene004004:CDS
ATATGATGCATCATAGGGAT+TGG 0.484646 4.3:-39342097 MS.gene004004:CDS
ATGAGAATGATTAGCAGAAC+CGG 0.486272 4.3:+39342355 None:intergenic
ATTTAATATGATGCATCATA+GGG 0.488329 4.3:-39342102 MS.gene004004:CDS
ATGCAGTTGATGAATGTTGA+AGG 0.492393 4.3:-39342518 MS.gene004004:CDS
TTCCGCTTTCAGCATCTCCC+CGG 0.493661 4.3:-39342888 MS.gene004004:CDS
ATCATACTCGAAGGTGGTGG+TGG 0.507063 4.3:+39342227 None:intergenic
CGTGTAACGGAGTGGGAAAA+GGG 0.517852 4.3:-39342971 MS.gene004004:CDS
TCTCTTCTCCGAAGCAACTC+CGG 0.518098 4.3:-39342815 MS.gene004004:CDS
AAAGCGGAAGCGAGCTCCGG+TGG 0.521066 4.3:+39342902 None:intergenic
GACGAAGAACCCGAGCGAGA+TGG 0.525147 4.3:-39342734 MS.gene004004:CDS
AATCATTCAAATGGAGGGCA+TGG 0.528339 4.3:-39342317 MS.gene004004:CDS
AGATTCTGTTACAGAAGAAG+TGG 0.528651 4.3:-39342678 MS.gene004004:CDS
CGAACCTGATCCATCTCGCT+CGG 0.534136 4.3:+39342724 None:intergenic
AAAAGATGATCAGAAGAAGA+AGG 0.541009 4.3:+39342413 None:intergenic
GAACCTGATCCATCTCGCTC+GGG 0.541572 4.3:+39342725 None:intergenic
ATGTCAATGCCGCCTATGAT+TGG 0.542928 4.3:-39342206 MS.gene004004:CDS
GACATATGAGGATGAGAATG+TGG 0.546426 4.3:+39342065 None:intergenic
ACGAAGAACGTGTAACGGAG+TGG 0.550873 4.3:-39342979 MS.gene004004:CDS
AATGCTAATCATTCAAATGG+AGG 0.554348 4.3:-39342323 MS.gene004004:CDS
ATCATCCACATGGGCATATG+GGG 0.557841 4.3:-39342175 MS.gene004004:CDS
CTAGAACCACCCGAAGGAGG+AGG 0.558990 4.3:+39342143 None:intergenic
CTATCATTGGAAGACATATG+AGG 0.559295 4.3:+39342053 None:intergenic
GGGATCAAAGCCATGGAAAG+AGG 0.559832 4.3:+39342923 None:intergenic
ATGCCGCCTATGATTGGTAT+GGG 0.560585 4.3:-39342200 MS.gene004004:CDS
GGTTGACGTCGAAGAGAGTA+CGG 0.568518 4.3:+39342858 None:intergenic
CTCCGGTGGGATCAAAGCCA+TGG 0.569390 4.3:+39342916 None:intergenic
GTTCGAATTCAAGAAAACAT+AGG 0.571081 4.3:-39342706 MS.gene004004:CDS
AATGCCGCCTATGATTGGTA+TGG 0.579585 4.3:-39342201 MS.gene004004:CDS
ATGCTAATCATTCAAATGGA+GGG 0.580774 4.3:-39342322 MS.gene004004:CDS
TCTTCTACTTCGCTGGACAC+GGG 0.581779 4.3:+39342767 None:intergenic
CTTTATTTGAAGAGAATGCA+AGG 0.583827 4.3:-39342452 MS.gene004004:CDS
AACAAACTGAATGGGTGAAG+AGG 0.590082 4.3:-39343011 None:intergenic
GGTGGTGGTGGATAAGGCAT+AGG 0.595338 4.3:+39342239 None:intergenic
CTCCGGGGAGATGCTGAAAG+CGG 0.595726 4.3:+39342886 None:intergenic
TGGAAAGAGGAGTGAGATCG+TGG 0.601806 4.3:+39342936 None:intergenic
TTTATTTGAAGAGAATGCAA+GGG 0.602218 4.3:-39342451 MS.gene004004:CDS
AAGAGAGAGTGTGTTGCGAG+AGG 0.612371 4.3:+39342832 None:intergenic
GACATCATACTCGAAGGTGG+TGG 0.615415 4.3:+39342224 None:intergenic
GAGCTAGAACCACCCGAAGG+AGG 0.617421 4.3:+39342140 None:intergenic
GGAGGGCATGGAAATGGACA+TGG 0.620162 4.3:-39342305 MS.gene004004:CDS
GGCATTGACATCATACTCGA+AGG 0.620560 4.3:+39342218 None:intergenic
CTCATCCCCATATGCCCATG+TGG 0.624417 4.3:+39342170 None:intergenic
GAGAGTACGGAGAGGCTCCG+GGG 0.626074 4.3:+39342871 None:intergenic
AGTTTGCCTCCTCCTTCGGG+TGG 0.628086 4.3:-39342149 MS.gene004004:CDS
CGAAGAACGTGTAACGGAGT+GGG 0.629837 4.3:-39342978 MS.gene004004:CDS
TGTGGGGTCCACACCAACCT+CGG 0.635154 4.3:+39342608 None:intergenic
AAGCGGAAGCGAGCTCCGGT+GGG 0.636737 4.3:+39342903 None:intergenic
AACAGACGAAGAACGTGTAA+CGG 0.639303 4.3:-39342984 MS.gene004004:CDS
GGATGAGAATGTGGATTCGG+AGG 0.643753 4.3:+39342074 None:intergenic
GGTATGGGACATCATCCACA+TGG 0.649470 4.3:-39342185 MS.gene004004:CDS
AAAGAGGAGTGAGATCGTGG+AGG 0.675435 4.3:+39342939 None:intergenic
ATTGACATCATACTCGAAGG+TGG 0.684359 4.3:+39342221 None:intergenic
AGAGTGTGTTGCGAGAGGCG+CGG 0.693296 4.3:+39342837 None:intergenic
TAAGAGCTAGAACCACCCGA+AGG 0.715458 4.3:+39342137 None:intergenic
AGATTCGGCTGTTAGAACAA+CGG 0.719098 4.3:-39342645 MS.gene004004:CDS
TCATCTTGGGATAAAGAACG+CGG 0.719383 4.3:-39342555 MS.gene004004:CDS
GTATGGGACATCATCCACAT+GGG 0.727717 4.3:-39342184 MS.gene004004:CDS
CCGGTTTCATGCAAACTCTG+CGG 0.740073 4.3:+39342374 None:intergenic
TGTCCCATACCAATCATAGG+CGG 0.741457 4.3:+39342197 None:intergenic
ACGTCGAAGAGAGTACGGAG+AGG 0.748301 4.3:+39342863 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATGATGCATCATA+GGG - chr4.3:39342942-39342961 MS.gene004004:CDS 20.0%
! CATTTAATATGATGCATCAT+AGG - chr4.3:39342941-39342960 MS.gene004004:CDS 25.0%
! CTATGATGCATCATATTAAA+TGG + chr4.3:39342943-39342962 None:intergenic 25.0%
! TCAAATGCTAATCATTCAAA+TGG - chr4.3:39342718-39342737 MS.gene004004:CDS 25.0%
! TTTATTTGAAGAGAATGCAA+GGG - chr4.3:39342593-39342612 MS.gene004004:CDS 25.0%
AAAAGATGATCAGAAGAAGA+AGG + chr4.3:39342634-39342653 None:intergenic 30.0%
AATGCTAATCATTCAAATGG+AGG - chr4.3:39342721-39342740 MS.gene004004:CDS 30.0%
ACAGCTACATAAAAGATTTG+TGG - chr4.3:39342456-39342475 MS.gene004004:CDS 30.0%
ATGCTAATCATTCAAATGGA+GGG - chr4.3:39342722-39342741 MS.gene004004:CDS 30.0%
GTTCGAATTCAAGAAAACAT+AGG - chr4.3:39342338-39342357 MS.gene004004:CDS 30.0%
! AAATCTTTTATGTAGCTGTG+GGG + chr4.3:39342455-39342474 None:intergenic 30.0%
! ACAAATCTTTTATGTAGCTG+TGG + chr4.3:39342457-39342476 None:intergenic 30.0%
! ATCAACTGCATAATCGTTTT+CGG + chr4.3:39342517-39342536 None:intergenic 30.0%
! CAAATCTTTTATGTAGCTGT+GGG + chr4.3:39342456-39342475 None:intergenic 30.0%
! CTTTATTTGAAGAGAATGCA+AGG - chr4.3:39342592-39342611 MS.gene004004:CDS 30.0%
AGATTCTGTTACAGAAGAAG+TGG - chr4.3:39342366-39342385 MS.gene004004:CDS 35.0%
ATATGATGCATCATAGGGAT+TGG - chr4.3:39342947-39342966 MS.gene004004:CDS 35.0%
ATGAGAATGATTAGCAGAAC+CGG + chr4.3:39342692-39342711 None:intergenic 35.0%
CTATCATTGGAAGACATATG+AGG + chr4.3:39342994-39343013 None:intergenic 35.0%
GATTTGATGAGTGATTGTTC+CGG + chr4.3:39342251-39342270 None:intergenic 35.0%
!! ATGCAGTTGATGAATGTTGA+AGG - chr4.3:39342526-39342545 MS.gene004004:CDS 35.0%
AACAGACGAAGAACGTGTAA+CGG - chr4.3:39342060-39342079 MS.gene004004:CDS 40.0%
AATCATTCAAATGGAGGGCA+TGG - chr4.3:39342727-39342746 MS.gene004004:CDS 40.0%
AGATTCGGCTGTTAGAACAA+CGG - chr4.3:39342399-39342418 MS.gene004004:CDS 40.0%
ATTGACATCATACTCGAAGG+TGG + chr4.3:39342826-39342845 None:intergenic 40.0%
GAACAACGGAGACGATAAAA+AGG - chr4.3:39342413-39342432 MS.gene004004:CDS 40.0%
GACATATGAGGATGAGAATG+TGG + chr4.3:39342982-39343001 None:intergenic 40.0%
TCATCTTGGGATAAAGAACG+CGG - chr4.3:39342489-39342508 MS.gene004004:CDS 40.0%
TGAGGATGAGAATGTGGATT+CGG + chr4.3:39342976-39342995 None:intergenic 40.0%
TGATGTCCCATACCAATCAT+AGG + chr4.3:39342853-39342872 None:intergenic 40.0%
TGGATGTTGTTGCTCATCTT+GGG - chr4.3:39342476-39342495 MS.gene004004:CDS 40.0%
TTCAACTTCTTCTACTTCGC+TGG + chr4.3:39342287-39342306 None:intergenic 40.0%
AATGCCGCCTATGATTGGTA+TGG - chr4.3:39342843-39342862 MS.gene004004:CDS 45.0%
ACATCATCCACATGGGCATA+TGG - chr4.3:39342867-39342886 MS.gene004004:CDS 45.0%
AGTGGAAGCTGAAGCAGATT+CGG - chr4.3:39342384-39342403 MS.gene004004:CDS 45.0%
ATCATCCACATGGGCATATG+GGG - chr4.3:39342869-39342888 MS.gene004004:CDS 45.0%
ATGCCGCCTATGATTGGTAT+GGG - chr4.3:39342844-39342863 MS.gene004004:CDS 45.0%
ATGTCAATGCCGCCTATGAT+TGG - chr4.3:39342838-39342857 MS.gene004004:CDS 45.0%
CATCATCCACATGGGCATAT+GGG - chr4.3:39342868-39342887 MS.gene004004:CDS 45.0%
GTATGGGACATCATCCACAT+GGG - chr4.3:39342860-39342879 MS.gene004004:CDS 45.0%
GTGGATGTTGTTGCTCATCT+TGG - chr4.3:39342475-39342494 MS.gene004004:CDS 45.0%
TGATTGTTCCGGAGTTGCTT+CGG + chr4.3:39342240-39342259 None:intergenic 45.0%
TGTCCCATACCAATCATAGG+CGG + chr4.3:39342850-39342869 None:intergenic 45.0%
TTCTTCTACTTCGCTGGACA+CGG + chr4.3:39342281-39342300 None:intergenic 45.0%
! TCAAATGGAGGGCATGGAAA+TGG - chr4.3:39342733-39342752 MS.gene004004:CDS 45.0%
!! GGCATTGACATCATACTCGA+AGG + chr4.3:39342829-39342848 None:intergenic 45.0%
AAAGAGGAGTGAGATCGTGG+AGG + chr4.3:39342108-39342127 None:intergenic 50.0%
ATGAGTTTGCCTCCTCCTTC+GGG - chr4.3:39342892-39342911 MS.gene004004:CDS 50.0%
CCGCAGAGTTTGCATGAAAC+CGG - chr4.3:39342670-39342689 MS.gene004004:CDS 50.0%
CCGGTTTCATGCAAACTCTG+CGG + chr4.3:39342673-39342692 None:intergenic 50.0%
CGATCTCACTCCTCTTTCCA+TGG - chr4.3:39342111-39342130 MS.gene004004:CDS 50.0%
GACATCATACTCGAAGGTGG+TGG + chr4.3:39342823-39342842 None:intergenic 50.0%
GACGATAAAAAGGCCGAGGT+TGG - chr4.3:39342423-39342442 MS.gene004004:CDS 50.0%
GAGTGAGATCGTGGAGGTTA+GGG + chr4.3:39342102-39342121 None:intergenic 50.0%
GATGAGTTTGCCTCCTCCTT+CGG - chr4.3:39342891-39342910 MS.gene004004:CDS 50.0%
GGATGAGAATGTGGATTCGG+AGG + chr4.3:39342973-39342992 None:intergenic 50.0%
GGGATCAAAGCCATGGAAAG+AGG + chr4.3:39342124-39342143 None:intergenic 50.0%
GGTATGGGACATCATCCACA+TGG - chr4.3:39342859-39342878 MS.gene004004:CDS 50.0%
GGTTGACGTCGAAGAGAGTA+CGG + chr4.3:39342189-39342208 None:intergenic 50.0%
TAAGAGCTAGAACCACCCGA+AGG + chr4.3:39342910-39342929 None:intergenic 50.0%
TCTCTTCTCCGAAGCAACTC+CGG - chr4.3:39342229-39342248 MS.gene004004:CDS 50.0%
TCTTCTACTTCGCTGGACAC+GGG + chr4.3:39342280-39342299 None:intergenic 50.0%
TGGAAAGAGGAGTGAGATCG+TGG + chr4.3:39342111-39342130 None:intergenic 50.0%
! AAGAGAGAGTGTGTTGCGAG+AGG + chr4.3:39342215-39342234 None:intergenic 50.0%
! ACGAAGAACGTGTAACGGAG+TGG - chr4.3:39342065-39342084 MS.gene004004:CDS 50.0%
! ACGTGTAACGGAGTGGGAAA+AGG - chr4.3:39342072-39342091 MS.gene004004:CDS 50.0%
! ATCATACTCGAAGGTGGTGG+TGG + chr4.3:39342820-39342839 None:intergenic 50.0%
! CGAAGAACGTGTAACGGAGT+GGG - chr4.3:39342066-39342085 MS.gene004004:CDS 50.0%
! CGTGTAACGGAGTGGGAAAA+GGG - chr4.3:39342073-39342092 MS.gene004004:CDS 50.0%
!! TAAAAAGGCCGAGGTTGGTG+TGG - chr4.3:39342428-39342447 MS.gene004004:CDS 50.0%
!! TTCCATGGCTTTGATCCCAC+CGG - chr4.3:39342126-39342145 MS.gene004004:CDS 50.0%
CGAACCTGATCCATCTCGCT+CGG + chr4.3:39342323-39342342 None:intergenic 55.0%
CGGAGACGATAAAAAGGCCG+AGG - chr4.3:39342419-39342438 MS.gene004004:CDS 55.0%
CTCATCCCCATATGCCCATG+TGG + chr4.3:39342877-39342896 None:intergenic 55.0%
GAACCTGATCCATCTCGCTC+GGG + chr4.3:39342322-39342341 None:intergenic 55.0%
GGAGTGAGATCGTGGAGGTT+AGG + chr4.3:39342103-39342122 None:intergenic 55.0%
TCATGCAAACTCTGCGGCAC+AGG + chr4.3:39342667-39342686 None:intergenic 55.0%
TTCCGCTTTCAGCATCTCCC+CGG - chr4.3:39342156-39342175 MS.gene004004:CDS 55.0%
! AAGAGAGTACGGAGAGGCTC+CGG + chr4.3:39342178-39342197 None:intergenic 55.0%
! ACGTCGAAGAGAGTACGGAG+AGG + chr4.3:39342184-39342203 None:intergenic 55.0%
! GGAGGGCATGGAAATGGACA+TGG - chr4.3:39342739-39342758 MS.gene004004:CDS 55.0%
!! CTCGAAGGTGGTGGTGGATA+AGG + chr4.3:39342814-39342833 None:intergenic 55.0%
!! GGTGGTGGTGGATAAGGCAT+AGG + chr4.3:39342808-39342827 None:intergenic 55.0%
AGAGTGTGTTGCGAGAGGCG+CGG + chr4.3:39342210-39342229 None:intergenic 60.0%
AGTTTGCCTCCTCCTTCGGG+TGG - chr4.3:39342895-39342914 MS.gene004004:CDS 60.0%
CTAGAACCACCCGAAGGAGG+AGG + chr4.3:39342904-39342923 None:intergenic 60.0%
CTCCGGGGAGATGCTGAAAG+CGG + chr4.3:39342161-39342180 None:intergenic 60.0%
CTCCGGTGGGATCAAAGCCA+TGG + chr4.3:39342131-39342150 None:intergenic 60.0%
CTGAAAGCGGAAGCGAGCTC+CGG + chr4.3:39342148-39342167 None:intergenic 60.0%
GAACCCGAGCGAGATGGATC+AGG - chr4.3:39342316-39342335 MS.gene004004:CDS 60.0%
GACGAAGAACCCGAGCGAGA+TGG - chr4.3:39342310-39342329 MS.gene004004:CDS 60.0%
GAGCTAGAACCACCCGAAGG+AGG + chr4.3:39342907-39342926 None:intergenic 60.0%
TGTGGGGTCCACACCAACCT+CGG + chr4.3:39342439-39342458 None:intergenic 60.0%
! AGAGAGTACGGAGAGGCTCC+GGG + chr4.3:39342177-39342196 None:intergenic 60.0%
AAAGCGGAAGCGAGCTCCGG+TGG + chr4.3:39342145-39342164 None:intergenic 65.0%
AAGCGGAAGCGAGCTCCGGT+GGG + chr4.3:39342144-39342163 None:intergenic 65.0%
! GAGAGTACGGAGAGGCTCCG+GGG + chr4.3:39342176-39342195 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 39342043 39343023 39342043 ID=MS.gene004004
chr4.3 mRNA 39342043 39343023 39342043 ID=MS.gene004004.t1;Parent=MS.gene004004
chr4.3 exon 39342043 39343023 39342043 ID=MS.gene004004.t1.exon1;Parent=MS.gene004004.t1
chr4.3 CDS 39342043 39343023 39342043 ID=cds.MS.gene004004.t1;Parent=MS.gene004004.t1
Gene Sequence

>MS.gene004004

ATGGGTGAAGAGGTTCGAACAGACGAAGAACGTGTAACGGAGTGGGAAAAGGGTCTCCCTAACCTCCACGATCTCACTCCTCTTTCCATGGCTTTGATCCCACCGGAGCTCGCTTCCGCTTTCAGCATCTCCCCGGAGCCTCTCCGTACTCTCTTCGACGTCAACCGCGCCTCTCGCAACACACTCTCTCTTCTCCGAAGCAACTCCGGAACAATCACTCATCAAATCAATCAACCCGTGTCCAGCGAAGTAGAAGAAGTTGAAGAAGACGAAGAACCCGAGCGAGATGGATCAGGTTCGAATTCAAGAAAACATAGGAAAATAGATTCTGTTACAGAAGAAGTGGAAGCTGAAGCAGATTCGGCTGTTAGAACAACGGAGACGATAAAAAGGCCGAGGTTGGTGTGGACCCCACAGCTACATAAAAGATTTGTGGATGTTGTTGCTCATCTTGGGATAAAGAACGCGGTTCCGAAAACGATTATGCAGTTGATGAATGTTGAAGGATTAACAAGAGAAAATGTTGCTAGTCATTTGCAGAAATATCGTCTTTATTTGAAGAGAATGCAAGGGCTTTCAAATGATGCACCTTCTTCTTCTGATCATCTTTTTGCTTCTACACCTGTGCCGCAGAGTTTGCATGAAACCGGTTCTGCTAATCATTCTCATTCACATTCAAATGCTAATCATTCAAATGGAGGGCATGGAAATGGACATGGAAATTCGCATTCTCATTCACATTCGCATCATTCTGTGACGATTCCTATGCCTTATCCACCACCACCTTCGAGTATGATGTCAATGCCGCCTATGATTGGTATGGGACATCATCCACATGGGCATATGGGGATGAGTTTGCCTCCTCCTTCGGGTGGTTCTAGCTCTTATCGCTCGCATCCATTTAATATGATGCATCATAGGGATTGGCCTCCGAATCCACATTCTCATCCTCATATGTCTTCCAATGATAGTAATAAATAA
Protein sequence

>MS.gene004004.t1

MGEEVRTDEERVTEWEKGLPNLHDLTPLSMALIPPELASAFSISPEPLRTLFDVNRASRNTLSLLRSNSGTITHQINQPVSSEVEEVEEDEEPERDGSGSNSRKHRKIDSVTEEVEAEADSAVRTTETIKRPRLVWTPQLHKRFVDVVAHLGIKNAVPKTIMQLMNVEGLTRENVASHLQKYRLYLKRMQGLSNDAPSSSDHLFASTPVPQSLHETGSANHSHSHSNANHSNGGHGNGHGNSHSHSHSHHSVTIPMPYPPPPSSMMSMPPMIGMGHHPHGHMGMSLPPPSGGSSSYRSHPFNMMHHRDWPPNPHSHPHMSSNDSNK