AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41134

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41134.t1 MTR_4g064730 89.939 328 15 2 1 326 1 312 3.22e-168 470
MS.gene41134.t1 MTR_7g089010 43.534 232 76 5 8 223 5 197 6.99e-43 150
MS.gene41134.t1 MTR_4g121020 66.176 68 23 0 126 193 106 173 4.73e-25 105
MS.gene41134.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.50e-25 105
MS.gene41134.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.56e-25 105
MS.gene41134.t1 MTR_2g034960 49.057 106 47 1 126 224 206 311 3.52e-24 103
MS.gene41134.t1 MTR_7g117705 46.281 121 63 2 130 248 190 310 3.37e-23 99.8
MS.gene41134.t1 MTR_3g106220 49.485 97 46 1 130 226 197 290 3.66e-23 100
MS.gene41134.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.59e-22 98.6
MS.gene41134.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.81e-22 98.6
MS.gene41134.t1 MTR_4g131570 68.852 61 19 0 128 188 200 260 1.65e-21 95.5
MS.gene41134.t1 MTR_1g032570 57.143 77 29 1 113 189 117 189 2.33e-21 95.1
MS.gene41134.t1 MTR_1g032570 57.143 77 29 1 113 189 186 258 3.18e-21 94.7
MS.gene41134.t1 MTR_1g013170 44.954 109 45 2 130 227 190 294 7.99e-21 93.2
MS.gene41134.t1 MTR_4g131580 68.852 61 19 0 128 188 197 257 1.62e-20 92.4
MS.gene41134.t1 MTR_8g079940 51.685 89 41 2 113 200 190 277 1.53e-19 89.7
MS.gene41134.t1 MTR_0450s0040 39.200 125 67 2 128 246 166 287 1.84e-19 88.2
MS.gene41134.t1 MTR_3g102600 58.333 60 25 0 130 189 82 141 3.98e-19 84.0
MS.gene41134.t1 MTR_1g013180 51.316 76 37 0 130 205 341 416 4.38e-19 88.6
MS.gene41134.t1 MTR_3g102600 58.333 60 25 0 130 189 131 190 5.02e-19 84.7
MS.gene41134.t1 MTR_7g026400 53.125 64 30 0 124 187 150 213 1.35e-18 87.0
MS.gene41134.t1 MTR_4g131600 57.812 64 27 0 126 189 196 259 2.37e-18 86.3
MS.gene41134.t1 MTR_3g086100 36.000 150 85 4 102 247 105 247 1.30e-17 83.6
MS.gene41134.t1 MTR_3g086100 36.000 150 85 4 102 247 176 318 1.41e-17 84.0
MS.gene41134.t1 MTR_4g021760 33.951 162 89 5 110 258 146 302 6.01e-17 80.5
MS.gene41134.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 2.40e-16 80.1
MS.gene41134.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 2.44e-16 80.1
MS.gene41134.t1 MTR_4g098870 37.500 120 64 1 76 184 283 402 2.86e-16 79.7
MS.gene41134.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 3.22e-16 79.7
MS.gene41134.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 3.36e-16 79.7
MS.gene41134.t1 MTR_3g102590 51.389 72 33 1 130 199 142 213 4.05e-16 77.0
MS.gene41134.t1 MTR_5g014040 53.333 60 28 0 130 189 310 369 2.77e-15 77.0
MS.gene41134.t1 MTR_2g090675 43.038 79 43 1 130 206 160 238 2.22e-14 73.9
MS.gene41134.t1 MTR_4g086835 49.206 63 32 0 127 189 270 332 3.16e-14 73.2
MS.gene41134.t1 MTR_4g078810 44.928 69 38 0 130 198 157 225 3.42e-14 73.2
MS.gene41134.t1 MTR_4g021790 47.826 69 36 0 120 188 157 225 8.25e-14 70.9
MS.gene41134.t1 MTR_5g041350 38.679 106 53 4 128 227 12 111 2.67e-13 70.1
MS.gene41134.t1 MTR_5g014040 49.231 65 28 1 130 189 270 334 2.70e-13 70.9
MS.gene41134.t1 MTR_3g102600 50.943 53 26 0 130 182 82 134 3.03e-13 66.6
MS.gene41134.t1 MTR_5g014040 49.231 65 28 1 130 189 310 374 3.17e-13 70.5
MS.gene41134.t1 MTR_3g102600 50.943 53 26 0 130 182 131 183 3.53e-13 67.4
MS.gene41134.t1 MTR_5g017980 48.333 60 31 0 130 189 191 250 5.23e-13 68.9
MS.gene41134.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 5.27e-13 69.3
MS.gene41134.t1 MTR_5g017980 48.333 60 31 0 130 189 192 251 5.52e-13 68.9
MS.gene41134.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 6.35e-13 69.3
MS.gene41134.t1 MTR_4g086835 48.438 64 32 1 127 189 270 333 6.81e-13 69.3
MS.gene41134.t1 MTR_1g090670 60.000 55 21 1 131 184 44 98 7.08e-13 68.6
MS.gene41134.t1 MTR_4g113140 40.404 99 52 3 119 210 218 316 7.80e-13 69.3
MS.gene41134.t1 MTR_7g098250 34.127 126 76 3 65 183 190 315 8.60e-13 69.3
MS.gene41134.t1 MTR_8g105600 56.140 57 25 0 134 190 190 246 9.19e-13 69.3
MS.gene41134.t1 MTR_2g027800 52.055 73 29 2 131 197 39 111 9.90e-13 68.2
MS.gene41134.t1 MTR_2g027800 60.000 55 21 1 131 184 39 93 1.26e-12 67.8
MS.gene41134.t1 MTR_2g086450 55.172 58 25 1 128 184 42 99 1.65e-12 68.2
MS.gene41134.t1 MTR_1g093080 43.243 74 41 1 127 199 207 280 1.91e-12 67.8
MS.gene41134.t1 MTR_4g021855 49.231 65 32 1 125 188 161 225 2.58e-12 67.0
MS.gene41134.t1 MTR_1g080330 50.685 73 34 2 112 183 157 228 2.75e-12 66.6
MS.gene41134.t1 MTR_1g080330 49.315 73 35 2 112 183 157 228 2.91e-12 67.4
MS.gene41134.t1 MTR_6g032990 47.222 72 36 2 128 198 39 109 3.35e-12 67.0
MS.gene41134.t1 MTR_7g069660 49.315 73 36 1 112 183 52 124 4.67e-12 66.6
MS.gene41134.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 5.94e-12 65.9
MS.gene41134.t1 MTR_6g045327 48.276 58 30 0 130 187 171 228 6.22e-12 66.2
MS.gene41134.t1 MTR_5g027440 54.545 55 24 1 131 184 25 79 8.07e-12 65.1
MS.gene41134.t1 MTR_2g027860 48.611 72 35 2 131 201 49 119 8.58e-12 65.5
MS.gene41134.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 8.64e-12 65.5
MS.gene41134.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 9.42e-12 65.9
MS.gene41134.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.51e-11 65.1
MS.gene41134.t1 MTR_5g017980 47.541 61 31 1 130 189 192 252 1.55e-11 64.7
MS.gene41134.t1 MTR_5g054300 42.105 76 43 1 130 204 197 272 1.60e-11 65.1
MS.gene41134.t1 MTR_1g053830 50.943 53 25 1 133 184 20 72 2.11e-11 60.1
MS.gene41134.t1 MTR_6g453220 63.830 47 17 0 148 194 380 426 3.39e-11 64.3
MS.gene41134.t1 MTR_0223s0040 49.231 65 32 1 120 183 7 71 3.72e-11 63.2
MS.gene41134.t1 MTR_3g102590 54.902 51 23 0 130 180 142 192 5.05e-11 61.6
MS.gene41134.t1 MTR_1g112370 50.794 63 30 1 122 183 13 75 5.32e-11 63.2
MS.gene41134.t1 MTR_4g081710 50.000 58 28 1 128 184 14 71 5.45e-11 63.2
MS.gene41134.t1 MTR_2g023580 48.148 81 39 3 105 183 29 108 7.07e-11 62.0
MS.gene41134.t1 MTR_2g084230 53.125 64 29 1 121 183 87 150 8.98e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41134.t1 AT3G46640 57.021 235 75 4 1 215 1 229 5.56e-78 241
MS.gene41134.t1 AT3G46640 57.021 235 75 4 1 215 1 229 5.56e-78 241
MS.gene41134.t1 AT3G46640 57.021 235 75 4 1 215 1 229 2.84e-77 239
MS.gene41134.t1 AT5G59570 54.331 254 91 8 11 250 25 267 4.06e-73 228
MS.gene41134.t1 AT5G59570 54.331 254 91 8 11 250 25 267 4.06e-73 228
MS.gene41134.t1 AT3G10760 47.596 208 80 5 8 210 5 188 3.51e-39 141
MS.gene41134.t1 AT5G05090 81.176 85 15 1 127 210 77 161 3.08e-38 137
MS.gene41134.t1 AT2G40970 72.340 94 17 1 126 210 100 193 4.39e-38 136
MS.gene41134.t1 AT3G16857 52.128 94 40 1 100 193 141 229 2.21e-24 103
MS.gene41134.t1 AT3G16857 52.128 94 40 1 100 193 211 299 2.69e-24 103
MS.gene41134.t1 AT3G16857 52.128 94 40 1 100 193 211 299 2.92e-24 103
MS.gene41134.t1 AT2G25180 39.264 163 78 4 32 194 117 258 4.44e-22 97.4
MS.gene41134.t1 AT2G01760 57.895 76 29 1 130 202 134 209 7.71e-22 94.4
MS.gene41134.t1 AT5G58080 55.696 79 30 1 128 201 191 269 1.05e-21 96.3
MS.gene41134.t1 AT2G01760 57.895 76 29 1 130 202 199 274 2.14e-21 94.0
MS.gene41134.t1 AT2G01760 57.895 76 29 1 130 202 227 302 3.77e-21 93.6
MS.gene41134.t1 AT4G31920 44.792 96 50 1 130 225 182 274 1.33e-18 87.0
MS.gene41134.t1 AT5G44190 50.000 74 35 1 130 203 146 217 1.94e-16 79.7
MS.gene41134.t1 AT2G20570 33.544 158 99 2 41 196 65 218 2.05e-16 80.1
MS.gene41134.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.12e-16 79.3
MS.gene41134.t1 AT5G07210 46.053 76 39 1 119 192 210 285 7.80e-16 78.6
MS.gene41134.t1 AT5G07210 46.053 76 39 1 119 192 218 293 7.93e-16 78.6
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 1.88e-15 77.4
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 1.88e-15 77.4
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 1.88e-15 77.4
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 1.88e-15 77.4
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 1.88e-15 77.4
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 1.88e-15 77.4
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.41e-15 76.6
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.41e-15 76.6
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.41e-15 76.6
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.41e-15 76.6
MS.gene41134.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.41e-15 76.6
MS.gene41134.t1 AT2G20570 36.885 122 68 2 80 196 117 234 4.91e-15 75.9
MS.gene41134.t1 AT1G67710 42.857 98 49 3 97 190 94 188 6.29e-15 75.5
MS.gene41134.t1 AT1G67710 42.857 98 49 3 97 190 85 179 6.68e-15 75.5
MS.gene41134.t1 AT1G67710 42.857 98 49 3 97 190 159 253 1.06e-14 75.1
MS.gene41134.t1 AT3G12730 52.055 73 33 2 113 184 6 77 1.90e-14 72.0
MS.gene41134.t1 AT2G27070 45.679 81 41 2 130 207 224 304 2.61e-14 73.9
MS.gene41134.t1 AT3G04030 49.275 69 34 1 128 196 42 109 8.13e-14 72.0
MS.gene41134.t1 AT1G79430 53.623 69 30 2 117 184 21 88 1.08e-13 71.6
MS.gene41134.t1 AT5G45580 56.364 55 23 1 131 184 7 61 4.29e-13 68.6
MS.gene41134.t1 AT3G04030 50.000 70 33 2 128 196 42 110 4.49e-13 69.7
MS.gene41134.t1 AT5G45580 56.364 55 23 1 131 184 23 77 5.33e-13 68.6
MS.gene41134.t1 AT3G04030 50.000 70 33 2 128 196 42 110 5.47e-13 69.7
MS.gene41134.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.45e-13 69.3
MS.gene41134.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.45e-13 69.3
MS.gene41134.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.48e-13 68.6
MS.gene41134.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.55e-13 68.6
MS.gene41134.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.97e-13 69.3
MS.gene41134.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.34e-13 69.3
MS.gene41134.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.34e-13 69.3
MS.gene41134.t1 AT4G13640 58.182 55 22 1 131 184 37 91 7.71e-13 68.2
MS.gene41134.t1 AT4G13640 58.182 55 22 1 131 184 37 91 8.30e-13 68.2
MS.gene41134.t1 AT1G13300 42.529 87 46 3 130 212 180 266 8.60e-13 68.6
MS.gene41134.t1 AT3G62670 55.932 59 21 2 130 183 210 268 8.70e-13 68.9
MS.gene41134.t1 AT2G03500 47.692 65 33 1 125 188 227 291 1.18e-12 68.6
MS.gene41134.t1 AT2G01060 45.055 91 45 3 118 203 2 92 1.83e-12 67.0
MS.gene41134.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.08e-11 65.9
MS.gene41134.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.08e-11 65.9
MS.gene41134.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.08e-11 65.9
MS.gene41134.t1 AT2G38300 40.196 102 57 2 107 207 30 128 1.25e-11 65.1
MS.gene41134.t1 AT1G49560 44.118 68 37 1 122 188 183 250 2.18e-11 64.3
MS.gene41134.t1 AT1G25550 48.333 60 30 1 130 188 208 267 3.69e-11 63.9
MS.gene41134.t1 AT5G49240 50.980 51 25 0 130 180 222 272 4.72e-11 63.2
MS.gene41134.t1 AT3G25790 40.449 89 49 3 135 219 200 288 6.95e-11 63.2

Find 92 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGATGCATCATATTAAA+TGG 0.267208 4.1:+36109303 None:intergenic
GGAGTGAGATCGTGGAGGTT+AGG 0.288087 4.1:+36110143 None:intergenic
CATTTAATATGATGCATCAT+AGG 0.291545 4.1:-36109302 MS.gene41134:CDS
GATGAGTTTGCCTCCTCCTT+CGG 0.309025 4.1:-36109352 MS.gene41134:CDS
GAGTGAGATCGTGGAGGTTA+GGG 0.316953 4.1:+36110144 None:intergenic
ACATCATCCACATGGGCATA+TGG 0.344716 4.1:-36109376 MS.gene41134:CDS
GTGGATGTTGTTGCTCATCT+TGG 0.347586 4.1:-36109768 MS.gene41134:CDS
ATGAGTTTGCCTCCTCCTTC+GGG 0.352579 4.1:-36109351 MS.gene41134:CDS
CTCGAAGGTGGTGGTGGATA+AGG 0.394424 4.1:+36109432 None:intergenic
GAACAACGGAGACGATAAAA+AGG 0.405625 4.1:-36109830 MS.gene41134:CDS
GTCTTCTACTTCGCTGGACA+CGG 0.409549 4.1:+36109965 None:intergenic
CATCATCCACATGGGCATAT+GGG 0.413116 4.1:-36109375 MS.gene41134:CDS
TCAAATGGAGGGCATGGAAA+TGG 0.414953 4.1:-36109510 MS.gene41134:CDS
AAGAGAGTACGGAGAGGCTC+CGG 0.421773 4.1:+36110068 None:intergenic
TCAAATGCTAATCATTCAAA+TGG 0.429533 4.1:-36109525 MS.gene41134:CDS
TTCAACGTCTTCTACTTCGC+TGG 0.432464 4.1:+36109959 None:intergenic
TGATTGTTCCGGAGTTGCTT+CGG 0.434817 4.1:+36110006 None:intergenic
TGAGGATGAGAATGTGGATT+CGG 0.435504 4.1:+36109270 None:intergenic
TGGATGTTGTTGCTCATCTT+GGG 0.442275 4.1:-36109767 MS.gene41134:CDS
CCGCAGAGTTTGCATGAAAC+CGG 0.443486 4.1:-36109573 MS.gene41134:CDS
TTCCATGGCTTTGATCCCAC+CGG 0.445349 4.1:-36110117 MS.gene41134:CDS
AGTGGAAGCTGAAGCAGATT+CGG 0.449529 4.1:-36109859 MS.gene41134:CDS
ACGTGTAACGGAGTGGGAAA+AGG 0.453107 4.1:-36110171 MS.gene41134:CDS
AGAGAGTACGGAGAGGCTCC+GGG 0.454039 4.1:+36110069 None:intergenic
GAACCCGAGCGAGATGGATC+AGG 0.456693 4.1:-36109927 MS.gene41134:CDS
CTGAAAGCGGAAGCGAGCTC+CGG 0.460721 4.1:+36110098 None:intergenic
TGATGTCCCATACCAATCAT+AGG 0.460751 4.1:+36109393 None:intergenic
TCATGCAAACTCTGCGGCAC+AGG 0.465750 4.1:+36109579 None:intergenic
GATTTGATGAGTGATTGTTC+CGG 0.471479 4.1:+36109995 None:intergenic
ACAGCTACATAAAAGATTTG+TGG 0.472539 4.1:-36109787 MS.gene41134:CDS
CGATCTCACTCCTCTTTCCA+TGG 0.482732 4.1:-36110132 MS.gene41134:CDS
ATATGATGCATCATAGGGAT+TGG 0.484646 4.1:-36109296 MS.gene41134:CDS
ATGAGAATGATTAGCAGAAC+CGG 0.486272 4.1:+36109554 None:intergenic
ATTTAATATGATGCATCATA+GGG 0.488329 4.1:-36109301 MS.gene41134:CDS
ATGCAGTTGATGAATGTTGA+AGG 0.492393 4.1:-36109717 MS.gene41134:CDS
TTCCGCTTTCAGCATCTCCC+CGG 0.493661 4.1:-36110087 MS.gene41134:CDS
ATCATACTCGAAGGTGGTGG+TGG 0.507063 4.1:+36109426 None:intergenic
CGTGTAACGGAGTGGGAAAA+GGG 0.517852 4.1:-36110170 MS.gene41134:CDS
TCTCTTCTCCGAAGCAACTC+CGG 0.518098 4.1:-36110014 MS.gene41134:CDS
CGAGCGAGATGGATCAGGTT+CGG 0.519486 4.1:-36109922 MS.gene41134:CDS
AAAGCGGAAGCGAGCTCCGG+TGG 0.521066 4.1:+36110101 None:intergenic
GACGAAGAACCCGAGCGAGA+TGG 0.525147 4.1:-36109933 MS.gene41134:CDS
AATCATTCAAATGGAGGGCA+TGG 0.528339 4.1:-36109516 MS.gene41134:CDS
AGATTCTGTTACAGAAGAAG+TGG 0.528651 4.1:-36109877 MS.gene41134:CDS
CGAACCTGATCCATCTCGCT+CGG 0.534136 4.1:+36109923 None:intergenic
AAAAGATGATCAGAAGAAGA+AGG 0.541009 4.1:+36109612 None:intergenic
GAACCTGATCCATCTCGCTC+GGG 0.541572 4.1:+36109924 None:intergenic
ATGTCAATGCCGCCTATGAT+TGG 0.542928 4.1:-36109405 MS.gene41134:CDS
GACATATGAGGATGAGAATG+TGG 0.546426 4.1:+36109264 None:intergenic
TCTTCTACTTCGCTGGACAC+GGG 0.550393 4.1:+36109966 None:intergenic
ACGAAGAACGTGTAACGGAG+TGG 0.550873 4.1:-36110178 MS.gene41134:CDS
AATGCTAATCATTCAAATGG+AGG 0.554348 4.1:-36109522 MS.gene41134:CDS
ATCATCCACATGGGCATATG+GGG 0.557841 4.1:-36109374 MS.gene41134:CDS
CTAGAACCACCCGAAGGAGG+AGG 0.558990 4.1:+36109342 None:intergenic
CTATCATTGGAAGACATATG+AGG 0.559295 4.1:+36109252 None:intergenic
GGGATCAAAGCCATGGAAAG+AGG 0.559832 4.1:+36110122 None:intergenic
ATGCCGCCTATGATTGGTAT+GGG 0.560585 4.1:-36109399 MS.gene41134:CDS
GGTTGACGTCGAAGAGAGTA+CGG 0.568518 4.1:+36110057 None:intergenic
CTCCGGTGGGATCAAAGCCA+TGG 0.569390 4.1:+36110115 None:intergenic
GTTCGGATTCAAGAAAACAT+AGG 0.571081 4.1:-36109905 MS.gene41134:CDS
AATGCCGCCTATGATTGGTA+TGG 0.579585 4.1:-36109400 MS.gene41134:CDS
ATGCTAATCATTCAAATGGA+GGG 0.580774 4.1:-36109521 MS.gene41134:CDS
CTTTATTTGAAGAGAATGCA+AGG 0.583827 4.1:-36109651 MS.gene41134:CDS
AACAAACTGAATGGGTGAAG+AGG 0.590082 4.1:-36110210 None:intergenic
GGTGGTGGTGGATAAGGCAT+AGG 0.595338 4.1:+36109438 None:intergenic
CTCCGGGGAGATGCTGAAAG+CGG 0.595726 4.1:+36110085 None:intergenic
TGGAAAGAGGAGTGAGATCG+TGG 0.601806 4.1:+36110135 None:intergenic
TTTATTTGAAGAGAATGCAA+GGG 0.602218 4.1:-36109650 MS.gene41134:CDS
AAGAGAGAGTGTGTTGCGAG+AGG 0.612371 4.1:+36110031 None:intergenic
GACATCATACTCGAAGGTGG+TGG 0.615415 4.1:+36109423 None:intergenic
GAGCTAGAACCACCCGAAGG+AGG 0.617421 4.1:+36109339 None:intergenic
GGAGGGCATGGAAATGGACA+TGG 0.620162 4.1:-36109504 MS.gene41134:CDS
GGCATTGACATCATACTCGA+AGG 0.620560 4.1:+36109417 None:intergenic
CTCATCCCCATATGCCCATG+TGG 0.624417 4.1:+36109369 None:intergenic
GAGAGTACGGAGAGGCTCCG+GGG 0.626074 4.1:+36110070 None:intergenic
AGTTTGCCTCCTCCTTCGGG+TGG 0.628086 4.1:-36109348 MS.gene41134:CDS
CGAAGAACGTGTAACGGAGT+GGG 0.629837 4.1:-36110177 MS.gene41134:CDS
TGTGGGGTCCACACCAACCT+CGG 0.635154 4.1:+36109807 None:intergenic
AAGCGGAAGCGAGCTCCGGT+GGG 0.636737 4.1:+36110102 None:intergenic
AACAGACGAAGAACGTGTAA+CGG 0.639303 4.1:-36110183 MS.gene41134:CDS
GGATGAGAATGTGGATTCGG+AGG 0.643753 4.1:+36109273 None:intergenic
GGTATGGGACATCATCCACA+TGG 0.649470 4.1:-36109384 MS.gene41134:CDS
AAAGAGGAGTGAGATCGTGG+AGG 0.675435 4.1:+36110138 None:intergenic
ATTGACATCATACTCGAAGG+TGG 0.684359 4.1:+36109420 None:intergenic
AGAGTGTGTTGCGAGAGGCG+CGG 0.693296 4.1:+36110036 None:intergenic
TAAGAGCTAGAACCACCCGA+AGG 0.715458 4.1:+36109336 None:intergenic
AGATTCGGCTGTTAGAACAA+CGG 0.719098 4.1:-36109844 MS.gene41134:CDS
TCATCTTGGGATAAAGAACG+CGG 0.719383 4.1:-36109754 MS.gene41134:CDS
GTATGGGACATCATCCACAT+GGG 0.727717 4.1:-36109383 MS.gene41134:CDS
CCGGTTTCATGCAAACTCTG+CGG 0.740073 4.1:+36109573 None:intergenic
TGTCCCATACCAATCATAGG+CGG 0.741457 4.1:+36109396 None:intergenic
ACGTCGAAGAGAGTACGGAG+AGG 0.748301 4.1:+36110062 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATGATGCATCATA+GGG - chr4.1:36110141-36110160 MS.gene41134:CDS 20.0%
! CATTTAATATGATGCATCAT+AGG - chr4.1:36110140-36110159 MS.gene41134:CDS 25.0%
! CTATGATGCATCATATTAAA+TGG + chr4.1:36110142-36110161 None:intergenic 25.0%
! TCAAATGCTAATCATTCAAA+TGG - chr4.1:36109917-36109936 MS.gene41134:CDS 25.0%
! TTTATTTGAAGAGAATGCAA+GGG - chr4.1:36109792-36109811 MS.gene41134:CDS 25.0%
AAAAGATGATCAGAAGAAGA+AGG + chr4.1:36109833-36109852 None:intergenic 30.0%
AATGCTAATCATTCAAATGG+AGG - chr4.1:36109920-36109939 MS.gene41134:CDS 30.0%
ACAGCTACATAAAAGATTTG+TGG - chr4.1:36109655-36109674 MS.gene41134:CDS 30.0%
ATGCTAATCATTCAAATGGA+GGG - chr4.1:36109921-36109940 MS.gene41134:CDS 30.0%
! AAATCTTTTATGTAGCTGTG+GGG + chr4.1:36109654-36109673 None:intergenic 30.0%
! ACAAATCTTTTATGTAGCTG+TGG + chr4.1:36109656-36109675 None:intergenic 30.0%
! ATCAACTGCATAATCGTTTT+CGG + chr4.1:36109716-36109735 None:intergenic 30.0%
! CAAATCTTTTATGTAGCTGT+GGG + chr4.1:36109655-36109674 None:intergenic 30.0%
! CTTTATTTGAAGAGAATGCA+AGG - chr4.1:36109791-36109810 MS.gene41134:CDS 30.0%
AGATTCTGTTACAGAAGAAG+TGG - chr4.1:36109565-36109584 MS.gene41134:CDS 35.0%
ATATGATGCATCATAGGGAT+TGG - chr4.1:36110146-36110165 MS.gene41134:CDS 35.0%
ATGAGAATGATTAGCAGAAC+CGG + chr4.1:36109891-36109910 None:intergenic 35.0%
CTATCATTGGAAGACATATG+AGG + chr4.1:36110193-36110212 None:intergenic 35.0%
GATTTGATGAGTGATTGTTC+CGG + chr4.1:36109450-36109469 None:intergenic 35.0%
GTTCGGATTCAAGAAAACAT+AGG - chr4.1:36109537-36109556 MS.gene41134:CDS 35.0%
!! ATGCAGTTGATGAATGTTGA+AGG - chr4.1:36109725-36109744 MS.gene41134:CDS 35.0%
AACAGACGAAGAACGTGTAA+CGG - chr4.1:36109259-36109278 MS.gene41134:CDS 40.0%
AATCATTCAAATGGAGGGCA+TGG - chr4.1:36109926-36109945 MS.gene41134:CDS 40.0%
AGATTCGGCTGTTAGAACAA+CGG - chr4.1:36109598-36109617 MS.gene41134:CDS 40.0%
ATTGACATCATACTCGAAGG+TGG + chr4.1:36110025-36110044 None:intergenic 40.0%
GAACAACGGAGACGATAAAA+AGG - chr4.1:36109612-36109631 MS.gene41134:CDS 40.0%
GACATATGAGGATGAGAATG+TGG + chr4.1:36110181-36110200 None:intergenic 40.0%
TCATCTTGGGATAAAGAACG+CGG - chr4.1:36109688-36109707 MS.gene41134:CDS 40.0%
TGAGGATGAGAATGTGGATT+CGG + chr4.1:36110175-36110194 None:intergenic 40.0%
TGATGTCCCATACCAATCAT+AGG + chr4.1:36110052-36110071 None:intergenic 40.0%
TGGATGTTGTTGCTCATCTT+GGG - chr4.1:36109675-36109694 MS.gene41134:CDS 40.0%
AATGCCGCCTATGATTGGTA+TGG - chr4.1:36110042-36110061 MS.gene41134:CDS 45.0%
ACATCATCCACATGGGCATA+TGG - chr4.1:36110066-36110085 MS.gene41134:CDS 45.0%
AGTGGAAGCTGAAGCAGATT+CGG - chr4.1:36109583-36109602 MS.gene41134:CDS 45.0%
ATCATCCACATGGGCATATG+GGG - chr4.1:36110068-36110087 MS.gene41134:CDS 45.0%
ATGCCGCCTATGATTGGTAT+GGG - chr4.1:36110043-36110062 MS.gene41134:CDS 45.0%
ATGTCAATGCCGCCTATGAT+TGG - chr4.1:36110037-36110056 MS.gene41134:CDS 45.0%
CATCATCCACATGGGCATAT+GGG - chr4.1:36110067-36110086 MS.gene41134:CDS 45.0%
GTATGGGACATCATCCACAT+GGG - chr4.1:36110059-36110078 MS.gene41134:CDS 45.0%
GTGGATGTTGTTGCTCATCT+TGG - chr4.1:36109674-36109693 MS.gene41134:CDS 45.0%
TGATTGTTCCGGAGTTGCTT+CGG + chr4.1:36109439-36109458 None:intergenic 45.0%
TGTCCCATACCAATCATAGG+CGG + chr4.1:36110049-36110068 None:intergenic 45.0%
TTCAACGTCTTCTACTTCGC+TGG + chr4.1:36109486-36109505 None:intergenic 45.0%
! TCAAATGGAGGGCATGGAAA+TGG - chr4.1:36109932-36109951 MS.gene41134:CDS 45.0%
!! GGCATTGACATCATACTCGA+AGG + chr4.1:36110028-36110047 None:intergenic 45.0%
AAAGAGGAGTGAGATCGTGG+AGG + chr4.1:36109307-36109326 None:intergenic 50.0%
ATGAGTTTGCCTCCTCCTTC+GGG - chr4.1:36110091-36110110 MS.gene41134:CDS 50.0%
CCGCAGAGTTTGCATGAAAC+CGG - chr4.1:36109869-36109888 MS.gene41134:CDS 50.0%
CCGGTTTCATGCAAACTCTG+CGG + chr4.1:36109872-36109891 None:intergenic 50.0%
CGATCTCACTCCTCTTTCCA+TGG - chr4.1:36109310-36109329 MS.gene41134:CDS 50.0%
GACATCATACTCGAAGGTGG+TGG + chr4.1:36110022-36110041 None:intergenic 50.0%
GACGATAAAAAGGCCGAGGT+TGG - chr4.1:36109622-36109641 MS.gene41134:CDS 50.0%
GAGTGAGATCGTGGAGGTTA+GGG + chr4.1:36109301-36109320 None:intergenic 50.0%
GATGAGTTTGCCTCCTCCTT+CGG - chr4.1:36110090-36110109 MS.gene41134:CDS 50.0%
GGATGAGAATGTGGATTCGG+AGG + chr4.1:36110172-36110191 None:intergenic 50.0%
GGGATCAAAGCCATGGAAAG+AGG + chr4.1:36109323-36109342 None:intergenic 50.0%
GGTATGGGACATCATCCACA+TGG - chr4.1:36110058-36110077 MS.gene41134:CDS 50.0%
GGTTGACGTCGAAGAGAGTA+CGG + chr4.1:36109388-36109407 None:intergenic 50.0%
GTCTTCTACTTCGCTGGACA+CGG + chr4.1:36109480-36109499 None:intergenic 50.0%
TAAGAGCTAGAACCACCCGA+AGG + chr4.1:36110109-36110128 None:intergenic 50.0%
TCTCTTCTCCGAAGCAACTC+CGG - chr4.1:36109428-36109447 MS.gene41134:CDS 50.0%
TCTTCTACTTCGCTGGACAC+GGG + chr4.1:36109479-36109498 None:intergenic 50.0%
TGGAAAGAGGAGTGAGATCG+TGG + chr4.1:36109310-36109329 None:intergenic 50.0%
! AAGAGAGAGTGTGTTGCGAG+AGG + chr4.1:36109414-36109433 None:intergenic 50.0%
! ACGAAGAACGTGTAACGGAG+TGG - chr4.1:36109264-36109283 MS.gene41134:CDS 50.0%
! ACGTGTAACGGAGTGGGAAA+AGG - chr4.1:36109271-36109290 MS.gene41134:CDS 50.0%
! ATCATACTCGAAGGTGGTGG+TGG + chr4.1:36110019-36110038 None:intergenic 50.0%
! CGAAGAACGTGTAACGGAGT+GGG - chr4.1:36109265-36109284 MS.gene41134:CDS 50.0%
! CGTGTAACGGAGTGGGAAAA+GGG - chr4.1:36109272-36109291 MS.gene41134:CDS 50.0%
!! TAAAAAGGCCGAGGTTGGTG+TGG - chr4.1:36109627-36109646 MS.gene41134:CDS 50.0%
!! TTCCATGGCTTTGATCCCAC+CGG - chr4.1:36109325-36109344 MS.gene41134:CDS 50.0%
CGAACCTGATCCATCTCGCT+CGG + chr4.1:36109522-36109541 None:intergenic 55.0%
CGAGCGAGATGGATCAGGTT+CGG - chr4.1:36109520-36109539 MS.gene41134:CDS 55.0%
CGGAGACGATAAAAAGGCCG+AGG - chr4.1:36109618-36109637 MS.gene41134:CDS 55.0%
CTCATCCCCATATGCCCATG+TGG + chr4.1:36110076-36110095 None:intergenic 55.0%
GAACCTGATCCATCTCGCTC+GGG + chr4.1:36109521-36109540 None:intergenic 55.0%
GGAGTGAGATCGTGGAGGTT+AGG + chr4.1:36109302-36109321 None:intergenic 55.0%
TCATGCAAACTCTGCGGCAC+AGG + chr4.1:36109866-36109885 None:intergenic 55.0%
TTCCGCTTTCAGCATCTCCC+CGG - chr4.1:36109355-36109374 MS.gene41134:CDS 55.0%
! AAGAGAGTACGGAGAGGCTC+CGG + chr4.1:36109377-36109396 None:intergenic 55.0%
! ACGTCGAAGAGAGTACGGAG+AGG + chr4.1:36109383-36109402 None:intergenic 55.0%
! GGAGGGCATGGAAATGGACA+TGG - chr4.1:36109938-36109957 MS.gene41134:CDS 55.0%
!! CTCGAAGGTGGTGGTGGATA+AGG + chr4.1:36110013-36110032 None:intergenic 55.0%
!! GGTGGTGGTGGATAAGGCAT+AGG + chr4.1:36110007-36110026 None:intergenic 55.0%
AGAGTGTGTTGCGAGAGGCG+CGG + chr4.1:36109409-36109428 None:intergenic 60.0%
AGTTTGCCTCCTCCTTCGGG+TGG - chr4.1:36110094-36110113 MS.gene41134:CDS 60.0%
CTAGAACCACCCGAAGGAGG+AGG + chr4.1:36110103-36110122 None:intergenic 60.0%
CTCCGGGGAGATGCTGAAAG+CGG + chr4.1:36109360-36109379 None:intergenic 60.0%
CTCCGGTGGGATCAAAGCCA+TGG + chr4.1:36109330-36109349 None:intergenic 60.0%
CTGAAAGCGGAAGCGAGCTC+CGG + chr4.1:36109347-36109366 None:intergenic 60.0%
GAACCCGAGCGAGATGGATC+AGG - chr4.1:36109515-36109534 MS.gene41134:CDS 60.0%
GACGAAGAACCCGAGCGAGA+TGG - chr4.1:36109509-36109528 MS.gene41134:CDS 60.0%
GAGCTAGAACCACCCGAAGG+AGG + chr4.1:36110106-36110125 None:intergenic 60.0%
TGTGGGGTCCACACCAACCT+CGG + chr4.1:36109638-36109657 None:intergenic 60.0%
! AGAGAGTACGGAGAGGCTCC+GGG + chr4.1:36109376-36109395 None:intergenic 60.0%
AAAGCGGAAGCGAGCTCCGG+TGG + chr4.1:36109344-36109363 None:intergenic 65.0%
AAGCGGAAGCGAGCTCCGGT+GGG + chr4.1:36109343-36109362 None:intergenic 65.0%
! GAGAGTACGGAGAGGCTCCG+GGG + chr4.1:36109375-36109394 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 36109242 36110222 36109242 ID=MS.gene41134
chr4.1 mRNA 36109242 36110222 36109242 ID=MS.gene41134.t1;Parent=MS.gene41134
chr4.1 exon 36109242 36110222 36109242 ID=MS.gene41134.t1.exon1;Parent=MS.gene41134.t1
chr4.1 CDS 36109242 36110222 36109242 ID=cds.MS.gene41134.t1;Parent=MS.gene41134.t1
Gene Sequence

>MS.gene41134

ATGGGTGAAGAGGTTCGAACAGACGAAGAACGTGTAACGGAGTGGGAAAAGGGTCTCCCTAACCTCCACGATCTCACTCCTCTTTCCATGGCTTTGATCCCACCGGAGCTCGCTTCCGCTTTCAGCATCTCCCCGGAGCCTCTCCGTACTCTCTTCGACGTCAACCGCGCCTCTCGCAACACACTCTCTCTTCTCCGAAGCAACTCCGGAACAATCACTCATCAAATCAATCAACCCGTGTCCAGCGAAGTAGAAGACGTTGAAGAAGACGAAGAACCCGAGCGAGATGGATCAGGTTCGGATTCAAGAAAACATAGGAAAATAGATTCTGTTACAGAAGAAGTGGAAGCTGAAGCAGATTCGGCTGTTAGAACAACGGAGACGATAAAAAGGCCGAGGTTGGTGTGGACCCCACAGCTACATAAAAGATTTGTGGATGTTGTTGCTCATCTTGGGATAAAGAACGCGGTTCCGAAAACGATTATGCAGTTGATGAATGTTGAAGGATTAACAAGAGAAAATGTTGCTAGTCATTTGCAGAAATATCGTCTTTATTTGAAGAGAATGCAAGGGCTTTCAAATGATGCACCTTCTTCTTCTGATCATCTTTTTGCTTCTACACCTGTGCCGCAGAGTTTGCATGAAACCGGTTCTGCTAATCATTCTCATTCACATTCAAATGCTAATCATTCAAATGGAGGGCATGGAAATGGACATGGAAATTCGCATTCTCATTCACATTCGCATCATTCTGTGACGATTCCTATGCCTTATCCACCACCACCTTCGAGTATGATGTCAATGCCGCCTATGATTGGTATGGGACATCATCCACATGGGCATATGGGGATGAGTTTGCCTCCTCCTTCGGGTGGTTCTAGCTCTTATCGCTCGCATCCATTTAATATGATGCATCATAGGGATTGGCCTCCGAATCCACATTCTCATCCTCATATGTCTTCCAATGATAGTAATAAATAA
Protein sequence

>MS.gene41134.t1

MGEEVRTDEERVTEWEKGLPNLHDLTPLSMALIPPELASAFSISPEPLRTLFDVNRASRNTLSLLRSNSGTITHQINQPVSSEVEDVEEDEEPERDGSGSDSRKHRKIDSVTEEVEAEADSAVRTTETIKRPRLVWTPQLHKRFVDVVAHLGIKNAVPKTIMQLMNVEGLTRENVASHLQKYRLYLKRMQGLSNDAPSSSDHLFASTPVPQSLHETGSANHSHSHSNANHSNGGHGNGHGNSHSHSHSHHSVTIPMPYPPPPSSMMSMPPMIGMGHHPHGHMGMSLPPPSGGSSSYRSHPFNMMHHRDWPPNPHSHPHMSSNDSNK