AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021153.t1 MTR_4g064730 90.244 328 14 2 1 326 1 312 0.0 587
MS.gene021153.t1 MTR_7g089010 43.534 232 76 5 8 223 5 197 4.83e-43 150
MS.gene021153.t1 MTR_4g121020 66.176 68 23 0 126 193 106 173 4.06e-25 105
MS.gene021153.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 4.92e-25 105
MS.gene021153.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.20e-25 105
MS.gene021153.t1 MTR_2g034960 49.057 106 47 1 126 224 206 311 3.66e-24 103
MS.gene021153.t1 MTR_7g117705 42.553 141 78 3 130 267 190 330 2.57e-23 100
MS.gene021153.t1 MTR_3g106220 49.485 97 46 1 130 226 197 290 3.66e-23 100
MS.gene021153.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.62e-22 98.6
MS.gene021153.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.79e-22 98.6
MS.gene021153.t1 MTR_4g131570 68.852 61 19 0 128 188 200 260 1.52e-21 95.5
MS.gene021153.t1 MTR_1g032570 57.143 77 29 1 113 189 117 189 2.08e-21 95.1
MS.gene021153.t1 MTR_1g032570 57.143 77 29 1 113 189 186 258 2.87e-21 94.7
MS.gene021153.t1 MTR_1g013170 44.954 109 45 2 130 227 190 294 8.14e-21 93.2
MS.gene021153.t1 MTR_4g131580 68.852 61 19 0 128 188 197 257 1.42e-20 92.8
MS.gene021153.t1 MTR_8g079940 48.571 105 46 3 97 200 180 277 7.25e-20 90.5
MS.gene021153.t1 MTR_0450s0040 39.200 125 67 2 128 246 166 287 1.95e-19 87.8
MS.gene021153.t1 MTR_3g102600 58.333 60 25 0 130 189 82 141 3.67e-19 84.0
MS.gene021153.t1 MTR_1g013180 51.316 76 37 0 130 205 341 416 3.85e-19 88.6
MS.gene021153.t1 MTR_3g102600 58.333 60 25 0 130 189 131 190 4.92e-19 84.7
MS.gene021153.t1 MTR_7g026400 53.125 64 30 0 124 187 150 213 1.42e-18 86.7
MS.gene021153.t1 MTR_4g131600 57.812 64 27 0 126 189 196 259 2.14e-18 86.3
MS.gene021153.t1 MTR_3g086100 35.526 152 87 4 100 247 103 247 7.22e-18 84.3
MS.gene021153.t1 MTR_3g086100 35.526 152 87 4 100 247 174 318 7.72e-18 84.7
MS.gene021153.t1 MTR_4g021760 32.768 177 101 5 95 258 131 302 3.74e-17 80.9
MS.gene021153.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 3.87e-16 79.3
MS.gene021153.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 3.99e-16 79.3
MS.gene021153.t1 MTR_3g102590 51.389 72 33 1 130 199 142 213 4.09e-16 77.0
MS.gene021153.t1 MTR_4g098870 37.500 120 64 1 76 184 283 402 4.66e-16 79.3
MS.gene021153.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 4.90e-16 79.3
MS.gene021153.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 5.00e-16 79.0
MS.gene021153.t1 MTR_5g014040 53.333 60 28 0 130 189 310 369 2.72e-15 77.0
MS.gene021153.t1 MTR_2g090675 43.038 79 43 1 130 206 160 238 2.00e-14 73.9
MS.gene021153.t1 MTR_4g086835 49.206 63 32 0 127 189 270 332 3.19e-14 73.2
MS.gene021153.t1 MTR_4g078810 44.928 69 38 0 130 198 157 225 3.55e-14 73.2
MS.gene021153.t1 MTR_4g021790 38.532 109 55 1 80 188 129 225 6.13e-14 71.2
MS.gene021153.t1 MTR_5g014040 49.231 65 28 1 130 189 270 334 2.56e-13 70.9
MS.gene021153.t1 MTR_5g041350 38.679 106 53 4 128 227 12 111 2.72e-13 70.1
MS.gene021153.t1 MTR_5g014040 49.231 65 28 1 130 189 310 374 2.84e-13 70.9
MS.gene021153.t1 MTR_3g102600 50.943 53 26 0 130 182 82 134 2.89e-13 66.6
MS.gene021153.t1 MTR_3g102600 50.943 53 26 0 130 182 131 183 3.59e-13 67.4
MS.gene021153.t1 MTR_5g017980 48.333 60 31 0 130 189 192 251 4.71e-13 69.3
MS.gene021153.t1 MTR_5g017980 48.333 60 31 0 130 189 191 250 4.72e-13 69.3
MS.gene021153.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 5.22e-13 69.3
MS.gene021153.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 6.17e-13 69.3
MS.gene021153.t1 MTR_4g086835 48.438 64 32 1 127 189 270 333 6.69e-13 69.3
MS.gene021153.t1 MTR_1g090670 60.000 55 21 1 131 184 44 98 7.21e-13 68.6
MS.gene021153.t1 MTR_4g113140 40.404 99 52 3 119 210 218 316 8.17e-13 69.3
MS.gene021153.t1 MTR_7g069660 42.202 109 47 3 82 183 25 124 8.82e-13 68.6
MS.gene021153.t1 MTR_8g105600 56.140 57 25 0 134 190 190 246 8.86e-13 69.3
MS.gene021153.t1 MTR_2g027800 52.055 73 29 2 131 197 39 111 1.00e-12 68.2
MS.gene021153.t1 MTR_2g027800 60.000 55 21 1 131 184 39 93 1.31e-12 67.8
MS.gene021153.t1 MTR_2g086450 55.172 58 25 1 128 184 42 99 1.54e-12 68.2
MS.gene021153.t1 MTR_1g093080 43.243 74 41 1 127 199 207 280 1.91e-12 67.8
MS.gene021153.t1 MTR_4g021855 40.625 96 55 2 95 188 130 225 1.96e-12 67.0
MS.gene021153.t1 MTR_1g080330 50.685 73 34 2 112 183 157 228 2.99e-12 66.6
MS.gene021153.t1 MTR_1g080330 49.315 73 35 2 112 183 157 228 3.05e-12 67.4
MS.gene021153.t1 MTR_6g032990 47.222 72 36 2 128 198 39 109 3.17e-12 67.4
MS.gene021153.t1 MTR_7g098250 39.080 87 52 1 98 183 229 315 3.44e-12 67.4
MS.gene021153.t1 MTR_6g045327 39.326 89 52 1 101 187 140 228 4.23e-12 66.6
MS.gene021153.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 6.17e-12 65.9
MS.gene021153.t1 MTR_2g027860 48.611 72 35 2 131 201 49 119 6.87e-12 65.9
MS.gene021153.t1 MTR_5g027440 54.545 55 24 1 131 184 25 79 7.70e-12 65.1
MS.gene021153.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 9.40e-12 65.5
MS.gene021153.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.01e-11 65.9
MS.gene021153.t1 MTR_5g017980 47.541 61 31 1 130 189 192 252 1.44e-11 64.7
MS.gene021153.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.52e-11 65.1
MS.gene021153.t1 MTR_5g054300 42.105 76 43 1 130 204 197 272 1.61e-11 65.1
MS.gene021153.t1 MTR_1g053830 50.943 53 25 1 133 184 20 72 1.99e-11 60.1
MS.gene021153.t1 MTR_6g453220 63.830 47 17 0 148 194 380 426 3.51e-11 64.3
MS.gene021153.t1 MTR_0223s0040 49.231 65 32 1 120 183 7 71 3.79e-11 63.2
MS.gene021153.t1 MTR_3g102590 54.902 51 23 0 130 180 142 192 5.10e-11 61.6
MS.gene021153.t1 MTR_1g112370 50.794 63 30 1 122 183 13 75 5.42e-11 63.2
MS.gene021153.t1 MTR_4g081710 50.000 58 28 1 128 184 14 71 5.60e-11 63.2
MS.gene021153.t1 MTR_2g023580 48.148 81 39 3 105 183 29 108 6.62e-11 62.0
MS.gene021153.t1 MTR_2g084230 53.125 64 29 1 121 183 87 150 9.49e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021153.t1 AT3G46640 56.596 235 76 4 1 215 1 229 3.86e-77 239
MS.gene021153.t1 AT3G46640 56.596 235 76 4 1 215 1 229 3.86e-77 239
MS.gene021153.t1 AT3G46640 56.596 235 76 4 1 215 1 229 1.95e-76 237
MS.gene021153.t1 AT5G59570 53.414 249 101 7 11 250 25 267 6.83e-73 227
MS.gene021153.t1 AT5G59570 53.414 249 101 7 11 250 25 267 6.83e-73 227
MS.gene021153.t1 AT3G10760 47.596 208 80 5 8 210 5 188 2.59e-39 141
MS.gene021153.t1 AT5G05090 47.196 214 56 6 1 210 1 161 2.13e-38 137
MS.gene021153.t1 AT2G40970 72.340 94 17 1 126 210 100 193 4.12e-38 136
MS.gene021153.t1 AT3G16857 52.128 94 40 1 100 193 141 229 1.47e-24 104
MS.gene021153.t1 AT3G16857 52.128 94 40 1 100 193 211 299 1.88e-24 104
MS.gene021153.t1 AT3G16857 52.128 94 40 1 100 193 211 299 2.08e-24 104
MS.gene021153.t1 AT2G25180 48.182 110 48 1 94 194 149 258 2.35e-22 98.2
MS.gene021153.t1 AT2G01760 57.895 76 29 1 130 202 134 209 7.49e-22 94.4
MS.gene021153.t1 AT5G58080 55.696 79 30 1 128 201 191 269 1.03e-21 96.3
MS.gene021153.t1 AT2G01760 57.895 76 29 1 130 202 199 274 2.22e-21 94.0
MS.gene021153.t1 AT2G01760 57.895 76 29 1 130 202 227 302 3.70e-21 93.6
MS.gene021153.t1 AT4G31920 44.792 96 50 1 130 225 182 274 1.21e-18 87.0
MS.gene021153.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.08e-16 79.3
MS.gene021153.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.09e-16 79.7
MS.gene021153.t1 AT2G20570 32.911 158 100 2 41 196 65 218 5.84e-16 78.6
MS.gene021153.t1 AT5G07210 42.268 97 50 2 98 192 193 285 6.61e-16 79.0
MS.gene021153.t1 AT5G07210 42.268 97 50 2 98 192 201 293 6.91e-16 79.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.65e-15 77.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.65e-15 77.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.65e-15 77.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.65e-15 77.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.65e-15 77.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 2.65e-15 77.0
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 4.30e-15 76.3
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 4.30e-15 76.3
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 4.30e-15 76.3
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 4.30e-15 76.3
MS.gene021153.t1 AT4G18020 29.680 219 120 5 84 293 242 435 4.30e-15 76.3
MS.gene021153.t1 AT2G20570 36.885 122 68 2 80 196 117 234 4.31e-15 75.9
MS.gene021153.t1 AT1G67710 43.478 92 48 2 100 190 100 188 1.02e-14 75.1
MS.gene021153.t1 AT1G67710 43.478 92 48 2 100 190 91 179 1.10e-14 74.7
MS.gene021153.t1 AT3G12730 52.055 73 33 2 113 184 6 77 1.73e-14 72.4
MS.gene021153.t1 AT1G67710 43.478 92 48 2 100 190 165 253 1.83e-14 74.3
MS.gene021153.t1 AT2G27070 45.679 81 41 2 130 207 224 304 2.50e-14 73.9
MS.gene021153.t1 AT3G04030 49.275 69 34 1 128 196 42 109 7.98e-14 72.0
MS.gene021153.t1 AT1G79430 53.623 69 30 2 117 184 21 88 1.00e-13 71.6
MS.gene021153.t1 AT5G45580 56.364 55 23 1 131 184 7 61 3.82e-13 68.9
MS.gene021153.t1 AT3G04030 50.000 70 33 2 128 196 42 110 4.54e-13 69.7
MS.gene021153.t1 AT5G45580 56.364 55 23 1 131 184 23 77 4.62e-13 68.9
MS.gene021153.t1 AT3G04030 50.000 70 33 2 128 196 42 110 5.18e-13 69.7
MS.gene021153.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.16e-13 69.3
MS.gene021153.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.16e-13 69.3
MS.gene021153.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.48e-13 68.6
MS.gene021153.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.49e-13 68.6
MS.gene021153.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.84e-13 69.3
MS.gene021153.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.21e-13 69.3
MS.gene021153.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.21e-13 69.3
MS.gene021153.t1 AT4G13640 58.182 55 22 1 131 184 37 91 7.71e-13 68.2
MS.gene021153.t1 AT4G13640 58.182 55 22 1 131 184 37 91 7.92e-13 68.2
MS.gene021153.t1 AT1G13300 42.529 87 46 3 130 212 180 266 8.85e-13 68.6
MS.gene021153.t1 AT3G62670 55.932 59 21 2 130 183 210 268 9.11e-13 68.9
MS.gene021153.t1 AT2G03500 47.692 65 33 1 125 188 227 291 1.12e-12 68.9
MS.gene021153.t1 AT2G01060 45.055 91 45 3 118 203 2 92 1.71e-12 67.4
MS.gene021153.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.17e-11 65.9
MS.gene021153.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.17e-11 65.9
MS.gene021153.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.17e-11 65.9
MS.gene021153.t1 AT2G38300 40.196 102 57 2 107 207 30 128 1.24e-11 65.1
MS.gene021153.t1 AT1G49560 44.118 68 37 1 122 188 183 250 2.18e-11 64.3
MS.gene021153.t1 AT1G25550 48.333 60 30 1 130 188 208 267 3.46e-11 63.9
MS.gene021153.t1 AT5G49240 30.070 143 80 4 43 180 145 272 4.07e-11 63.2
MS.gene021153.t1 AT3G25790 40.449 89 49 3 135 219 200 288 7.21e-11 63.2

Find 90 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGATGCATCATATTAAA+TGG 0.267208 4.4:+41673553 None:intergenic
GGAGTGAGATCGTGGAGGTT+AGG 0.288087 4.4:+41674393 None:intergenic
CATTTAATATGATGCATCAT+AGG 0.291545 4.4:-41673552 MS.gene021153:CDS
GATGAGTTTGCCTCCTCCTT+CGG 0.309025 4.4:-41673602 MS.gene021153:CDS
GAGTGAGATCGTGGAGGTTA+GGG 0.316953 4.4:+41674394 None:intergenic
ACATCATCCACATGGGCATA+TGG 0.344716 4.4:-41673626 MS.gene021153:CDS
GTGGATGTTGTTGCTCATCT+TGG 0.347586 4.4:-41674018 MS.gene021153:CDS
ATGAGTTTGCCTCCTCCTTC+GGG 0.352579 4.4:-41673601 MS.gene021153:CDS
TTCAACTTCTTCTACTTCGC+TGG 0.386278 4.4:+41674209 None:intergenic
GAACAACGGAGACGATAAAA+AGG 0.405625 4.4:-41674080 MS.gene021153:CDS
CATCATCCACATGGGCATAT+GGG 0.413116 4.4:-41673625 MS.gene021153:CDS
TCGAATGGAGGGCATGGAAA+TGG 0.414953 4.4:-41673760 MS.gene021153:CDS
AAGAGAGTACGGAGAGGCTC+CGG 0.421773 4.4:+41674318 None:intergenic
GTCGAAGGTGGTGGTGGATA+AGG 0.424874 4.4:+41673682 None:intergenic
TGATTGTTCCGGAGTTGCTT+CGG 0.434817 4.4:+41674256 None:intergenic
TGAGGATGAGAATGTGGATT+CGG 0.435504 4.4:+41673520 None:intergenic
TGGATGTTGTTGCTCATCTT+GGG 0.442275 4.4:-41674017 MS.gene021153:CDS
TTCTTCTACTTCGCTGGACA+CGG 0.442290 4.4:+41674215 None:intergenic
CCGCAGAGTTTGCATGAAAC+CGG 0.443486 4.4:-41673823 MS.gene021153:CDS
TTCCATGGCTTTGATCCCAC+CGG 0.445349 4.4:-41674367 MS.gene021153:CDS
AGTGGAAGCTGAAGCAGATT+CGG 0.449529 4.4:-41674109 MS.gene021153:CDS
ACGTGTAACGGAGTGGGAAA+AGG 0.453107 4.4:-41674421 MS.gene021153:CDS
AGAGAGTACGGAGAGGCTCC+GGG 0.454039 4.4:+41674319 None:intergenic
GAACCCGAGCGAGATGGATC+AGG 0.456693 4.4:-41674177 MS.gene021153:CDS
CTGAAAGCGGAAGCGAGCTC+CGG 0.460721 4.4:+41674348 None:intergenic
TGATGTCCCATACCAATCAT+AGG 0.460751 4.4:+41673643 None:intergenic
TCATGCAAACTCTGCGGCAC+AGG 0.465750 4.4:+41673829 None:intergenic
GATTTGATGAGTGATTGTTC+CGG 0.471479 4.4:+41674245 None:intergenic
ACAGCTACATAAAAGATTTG+TGG 0.472539 4.4:-41674037 MS.gene021153:CDS
CGATCTCACTCCTCTTTCCA+TGG 0.482732 4.4:-41674382 MS.gene021153:CDS
ATATGATGCATCATAGGGAT+TGG 0.484646 4.4:-41673546 MS.gene021153:CDS
ATGAGAATGATTAGCAGAAC+CGG 0.486272 4.4:+41673804 None:intergenic
ATTTAATATGATGCATCATA+GGG 0.488329 4.4:-41673551 MS.gene021153:CDS
ATGCAGTTGATGAATGTTGA+AGG 0.492393 4.4:-41673967 MS.gene021153:CDS
TTCCGCTTTCAGCATCTCCC+CGG 0.493661 4.4:-41674337 MS.gene021153:CDS
ATCATAGTCGAAGGTGGTGG+TGG 0.494086 4.4:+41673676 None:intergenic
CGTGTAACGGAGTGGGAAAA+GGG 0.517852 4.4:-41674420 MS.gene021153:CDS
TCTCTTCTCCGAAGCAACTC+CGG 0.518098 4.4:-41674264 MS.gene021153:CDS
AAAGCGGAAGCGAGCTCCGG+TGG 0.521066 4.4:+41674351 None:intergenic
GACGAAGAACCCGAGCGAGA+TGG 0.525147 4.4:-41674183 MS.gene021153:CDS
AGATTCTGTTACAGAAGAAG+TGG 0.528651 4.4:-41674127 MS.gene021153:CDS
CGAACCTGATCCATCTCGCT+CGG 0.534136 4.4:+41674173 None:intergenic
TCAAATGCTAATCATTCGAA+TGG 0.540654 4.4:-41673775 MS.gene021153:CDS
AAAAGATGATCAGAAGAAGA+AGG 0.541009 4.4:+41673862 None:intergenic
GAACCTGATCCATCTCGCTC+GGG 0.541572 4.4:+41674174 None:intergenic
ATGTCAATGCCGCCTATGAT+TGG 0.542928 4.4:-41673655 MS.gene021153:CDS
GACATATGAGGATGAGAATG+TGG 0.546426 4.4:+41673514 None:intergenic
ACGAAGAACGTGTAACGGAG+TGG 0.550873 4.4:-41674428 MS.gene021153:CDS
ATCATCCACATGGGCATATG+GGG 0.557841 4.4:-41673624 MS.gene021153:CDS
CTAGAACCACCCGAAGGAGG+AGG 0.558990 4.4:+41673592 None:intergenic
CTATCATTGGAAGACATATG+AGG 0.559295 4.4:+41673502 None:intergenic
GGGATCAAAGCCATGGAAAG+AGG 0.559832 4.4:+41674372 None:intergenic
ATGCCGCCTATGATTGGTAT+GGG 0.560585 4.4:-41673649 MS.gene021153:CDS
GGTTGACGTCGAAGAGAGTA+CGG 0.568518 4.4:+41674307 None:intergenic
CTCCGGTGGGATCAAAGCCA+TGG 0.569390 4.4:+41674365 None:intergenic
GTTCGAATTCAAGAAAACAT+AGG 0.571081 4.4:-41674155 MS.gene021153:CDS
ATGCTAATCATTCGAATGGA+GGG 0.576619 4.4:-41673771 MS.gene021153:CDS
AATCATTCGAATGGAGGGCA+TGG 0.576636 4.4:-41673766 MS.gene021153:CDS
AATGCCGCCTATGATTGGTA+TGG 0.579585 4.4:-41673650 MS.gene021153:CDS
TCTTCTACTTCGCTGGACAC+GGG 0.581779 4.4:+41674216 None:intergenic
GACATCATAGTCGAAGGTGG+TGG 0.587152 4.4:+41673673 None:intergenic
AACAAACTGAATGGGTGAAG+AGG 0.590082 4.4:-41674460 None:intergenic
CTTTATTTGAAGAGAATGCA+AGG 0.591058 4.4:-41673901 MS.gene021153:CDS
GGTGGTGGTGGATAAGGCAT+AGG 0.595338 4.4:+41673688 None:intergenic
CTCCGGGGAGATGCTGAAAG+CGG 0.595726 4.4:+41674335 None:intergenic
TGGAAAGAGGAGTGAGATCG+TGG 0.601806 4.4:+41674385 None:intergenic
GGCATTGACATCATAGTCGA+AGG 0.602892 4.4:+41673667 None:intergenic
AATGCTAATCATTCGAATGG+AGG 0.610766 4.4:-41673772 MS.gene021153:CDS
AAGAGAGAGTGTGTTGCGAG+AGG 0.612371 4.4:+41674281 None:intergenic
GAGCTAGAACCACCCGAAGG+AGG 0.617421 4.4:+41673589 None:intergenic
GGAGGGCATGGAAATGGACA+TGG 0.620162 4.4:-41673754 MS.gene021153:CDS
CTCATCCCCATATGCCCATG+TGG 0.624417 4.4:+41673619 None:intergenic
GAGAGTACGGAGAGGCTCCG+GGG 0.626074 4.4:+41674320 None:intergenic
AGTTTGCCTCCTCCTTCGGG+TGG 0.628086 4.4:-41673598 MS.gene021153:CDS
CGAAGAACGTGTAACGGAGT+GGG 0.629837 4.4:-41674427 MS.gene021153:CDS
TGTGGGGTCCACACCAACCT+CGG 0.635154 4.4:+41674057 None:intergenic
AAGCGGAAGCGAGCTCCGGT+GGG 0.636737 4.4:+41674352 None:intergenic
AACAGACGAAGAACGTGTAA+CGG 0.639303 4.4:-41674433 MS.gene021153:CDS
GGATGAGAATGTGGATTCGG+AGG 0.643753 4.4:+41673523 None:intergenic
GGTATGGGACATCATCCACA+TGG 0.649470 4.4:-41673634 MS.gene021153:CDS
AAAGAGGAGTGAGATCGTGG+AGG 0.675435 4.4:+41674388 None:intergenic
ATTGACATCATAGTCGAAGG+TGG 0.681193 4.4:+41673670 None:intergenic
AGAGTGTGTTGCGAGAGGCG+CGG 0.693296 4.4:+41674286 None:intergenic
TAAGAGCTAGAACCACCCGA+AGG 0.715458 4.4:+41673586 None:intergenic
AGATTCGGCTGTTAGAACAA+CGG 0.719098 4.4:-41674094 MS.gene021153:CDS
TCATCTTGGGATAAAGAACG+CGG 0.719383 4.4:-41674004 MS.gene021153:CDS
GTATGGGACATCATCCACAT+GGG 0.727717 4.4:-41673633 MS.gene021153:CDS
CCGGTTTCATGCAAACTCTG+CGG 0.740073 4.4:+41673823 None:intergenic
TGTCCCATACCAATCATAGG+CGG 0.741457 4.4:+41673646 None:intergenic
ACGTCGAAGAGAGTACGGAG+AGG 0.748301 4.4:+41674312 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATGATGCATCATA+GGG - chr4.4:41674391-41674410 MS.gene021153:CDS 20.0%
! CATTTAATATGATGCATCAT+AGG - chr4.4:41674390-41674409 MS.gene021153:CDS 25.0%
! CTATGATGCATCATATTAAA+TGG + chr4.4:41674392-41674411 None:intergenic 25.0%
AAAAGATGATCAGAAGAAGA+AGG + chr4.4:41674083-41674102 None:intergenic 30.0%
ACAGCTACATAAAAGATTTG+TGG - chr4.4:41673905-41673924 MS.gene021153:CDS 30.0%
GTTCGAATTCAAGAAAACAT+AGG - chr4.4:41673787-41673806 MS.gene021153:CDS 30.0%
TCAAATGCTAATCATTCGAA+TGG - chr4.4:41674167-41674186 MS.gene021153:CDS 30.0%
! AAATCTTTTATGTAGCTGTG+GGG + chr4.4:41673904-41673923 None:intergenic 30.0%
! ACAAATCTTTTATGTAGCTG+TGG + chr4.4:41673906-41673925 None:intergenic 30.0%
! ATCAACTGCATAATCGTTTT+CGG + chr4.4:41673966-41673985 None:intergenic 30.0%
! CAAATCTTTTATGTAGCTGT+GGG + chr4.4:41673905-41673924 None:intergenic 30.0%
! CTTTATTTGAAGAGAATGCA+AGG - chr4.4:41674041-41674060 MS.gene021153:CDS 30.0%
AATGCTAATCATTCGAATGG+AGG - chr4.4:41674170-41674189 MS.gene021153:CDS 35.0%
AGATTCTGTTACAGAAGAAG+TGG - chr4.4:41673815-41673834 MS.gene021153:CDS 35.0%
ATATGATGCATCATAGGGAT+TGG - chr4.4:41674396-41674415 MS.gene021153:CDS 35.0%
ATGAGAATGATTAGCAGAAC+CGG + chr4.4:41674141-41674160 None:intergenic 35.0%
ATGCTAATCATTCGAATGGA+GGG - chr4.4:41674171-41674190 MS.gene021153:CDS 35.0%
CTATCATTGGAAGACATATG+AGG + chr4.4:41674443-41674462 None:intergenic 35.0%
GATTTGATGAGTGATTGTTC+CGG + chr4.4:41673700-41673719 None:intergenic 35.0%
!! ATGCAGTTGATGAATGTTGA+AGG - chr4.4:41673975-41673994 MS.gene021153:CDS 35.0%
AACAGACGAAGAACGTGTAA+CGG - chr4.4:41673509-41673528 MS.gene021153:CDS 40.0%
AGATTCGGCTGTTAGAACAA+CGG - chr4.4:41673848-41673867 MS.gene021153:CDS 40.0%
ATTGACATCATAGTCGAAGG+TGG + chr4.4:41674275-41674294 None:intergenic 40.0%
GAACAACGGAGACGATAAAA+AGG - chr4.4:41673862-41673881 MS.gene021153:CDS 40.0%
GACATATGAGGATGAGAATG+TGG + chr4.4:41674431-41674450 None:intergenic 40.0%
TCATCTTGGGATAAAGAACG+CGG - chr4.4:41673938-41673957 MS.gene021153:CDS 40.0%
TGAGGATGAGAATGTGGATT+CGG + chr4.4:41674425-41674444 None:intergenic 40.0%
TGATGTCCCATACCAATCAT+AGG + chr4.4:41674302-41674321 None:intergenic 40.0%
TGGATGTTGTTGCTCATCTT+GGG - chr4.4:41673925-41673944 MS.gene021153:CDS 40.0%
TTCAACTTCTTCTACTTCGC+TGG + chr4.4:41673736-41673755 None:intergenic 40.0%
AATCATTCGAATGGAGGGCA+TGG - chr4.4:41674176-41674195 MS.gene021153:CDS 45.0%
AATGCCGCCTATGATTGGTA+TGG - chr4.4:41674292-41674311 MS.gene021153:CDS 45.0%
ACATCATCCACATGGGCATA+TGG - chr4.4:41674316-41674335 MS.gene021153:CDS 45.0%
AGTGGAAGCTGAAGCAGATT+CGG - chr4.4:41673833-41673852 MS.gene021153:CDS 45.0%
ATCATCCACATGGGCATATG+GGG - chr4.4:41674318-41674337 MS.gene021153:CDS 45.0%
ATGCCGCCTATGATTGGTAT+GGG - chr4.4:41674293-41674312 MS.gene021153:CDS 45.0%
ATGTCAATGCCGCCTATGAT+TGG - chr4.4:41674287-41674306 MS.gene021153:CDS 45.0%
CATCATCCACATGGGCATAT+GGG - chr4.4:41674317-41674336 MS.gene021153:CDS 45.0%
GTATGGGACATCATCCACAT+GGG - chr4.4:41674309-41674328 MS.gene021153:CDS 45.0%
GTGGATGTTGTTGCTCATCT+TGG - chr4.4:41673924-41673943 MS.gene021153:CDS 45.0%
TGATTGTTCCGGAGTTGCTT+CGG + chr4.4:41673689-41673708 None:intergenic 45.0%
TGTCCCATACCAATCATAGG+CGG + chr4.4:41674299-41674318 None:intergenic 45.0%
TTCTTCTACTTCGCTGGACA+CGG + chr4.4:41673730-41673749 None:intergenic 45.0%
!! GGCATTGACATCATAGTCGA+AGG + chr4.4:41674278-41674297 None:intergenic 45.0%
AAAGAGGAGTGAGATCGTGG+AGG + chr4.4:41673557-41673576 None:intergenic 50.0%
ATGAGTTTGCCTCCTCCTTC+GGG - chr4.4:41674341-41674360 MS.gene021153:CDS 50.0%
CCGCAGAGTTTGCATGAAAC+CGG - chr4.4:41674119-41674138 MS.gene021153:CDS 50.0%
CCGGTTTCATGCAAACTCTG+CGG + chr4.4:41674122-41674141 None:intergenic 50.0%
CGATCTCACTCCTCTTTCCA+TGG - chr4.4:41673560-41673579 MS.gene021153:CDS 50.0%
GACATCATAGTCGAAGGTGG+TGG + chr4.4:41674272-41674291 None:intergenic 50.0%
GACGATAAAAAGGCCGAGGT+TGG - chr4.4:41673872-41673891 MS.gene021153:CDS 50.0%
GAGTGAGATCGTGGAGGTTA+GGG + chr4.4:41673551-41673570 None:intergenic 50.0%
GATGAGTTTGCCTCCTCCTT+CGG - chr4.4:41674340-41674359 MS.gene021153:CDS 50.0%
GGATGAGAATGTGGATTCGG+AGG + chr4.4:41674422-41674441 None:intergenic 50.0%
GGGATCAAAGCCATGGAAAG+AGG + chr4.4:41673573-41673592 None:intergenic 50.0%
GGTATGGGACATCATCCACA+TGG - chr4.4:41674308-41674327 MS.gene021153:CDS 50.0%
GGTTGACGTCGAAGAGAGTA+CGG + chr4.4:41673638-41673657 None:intergenic 50.0%
TAAGAGCTAGAACCACCCGA+AGG + chr4.4:41674359-41674378 None:intergenic 50.0%
TCTCTTCTCCGAAGCAACTC+CGG - chr4.4:41673678-41673697 MS.gene021153:CDS 50.0%
TCTTCTACTTCGCTGGACAC+GGG + chr4.4:41673729-41673748 None:intergenic 50.0%
TGGAAAGAGGAGTGAGATCG+TGG + chr4.4:41673560-41673579 None:intergenic 50.0%
! AAGAGAGAGTGTGTTGCGAG+AGG + chr4.4:41673664-41673683 None:intergenic 50.0%
! ACGAAGAACGTGTAACGGAG+TGG - chr4.4:41673514-41673533 MS.gene021153:CDS 50.0%
! ACGTGTAACGGAGTGGGAAA+AGG - chr4.4:41673521-41673540 MS.gene021153:CDS 50.0%
! ATCATAGTCGAAGGTGGTGG+TGG + chr4.4:41674269-41674288 None:intergenic 50.0%
! CGAAGAACGTGTAACGGAGT+GGG - chr4.4:41673515-41673534 MS.gene021153:CDS 50.0%
! CGTGTAACGGAGTGGGAAAA+GGG - chr4.4:41673522-41673541 MS.gene021153:CDS 50.0%
! TCGAATGGAGGGCATGGAAA+TGG - chr4.4:41674182-41674201 MS.gene021153:CDS 50.0%
!! TAAAAAGGCCGAGGTTGGTG+TGG - chr4.4:41673877-41673896 MS.gene021153:CDS 50.0%
!! TTCCATGGCTTTGATCCCAC+CGG - chr4.4:41673575-41673594 MS.gene021153:CDS 50.0%
CGAACCTGATCCATCTCGCT+CGG + chr4.4:41673772-41673791 None:intergenic 55.0%
CGGAGACGATAAAAAGGCCG+AGG - chr4.4:41673868-41673887 MS.gene021153:CDS 55.0%
CTCATCCCCATATGCCCATG+TGG + chr4.4:41674326-41674345 None:intergenic 55.0%
GAACCTGATCCATCTCGCTC+GGG + chr4.4:41673771-41673790 None:intergenic 55.0%
GGAGTGAGATCGTGGAGGTT+AGG + chr4.4:41673552-41673571 None:intergenic 55.0%
TCATGCAAACTCTGCGGCAC+AGG + chr4.4:41674116-41674135 None:intergenic 55.0%
TTCCGCTTTCAGCATCTCCC+CGG - chr4.4:41673605-41673624 MS.gene021153:CDS 55.0%
! AAGAGAGTACGGAGAGGCTC+CGG + chr4.4:41673627-41673646 None:intergenic 55.0%
! ACGTCGAAGAGAGTACGGAG+AGG + chr4.4:41673633-41673652 None:intergenic 55.0%
! GGAGGGCATGGAAATGGACA+TGG - chr4.4:41674188-41674207 MS.gene021153:CDS 55.0%
!! GGTGGTGGTGGATAAGGCAT+AGG + chr4.4:41674257-41674276 None:intergenic 55.0%
!! GTCGAAGGTGGTGGTGGATA+AGG + chr4.4:41674263-41674282 None:intergenic 55.0%
AGAGTGTGTTGCGAGAGGCG+CGG + chr4.4:41673659-41673678 None:intergenic 60.0%
AGTTTGCCTCCTCCTTCGGG+TGG - chr4.4:41674344-41674363 MS.gene021153:CDS 60.0%
CTAGAACCACCCGAAGGAGG+AGG + chr4.4:41674353-41674372 None:intergenic 60.0%
CTCCGGGGAGATGCTGAAAG+CGG + chr4.4:41673610-41673629 None:intergenic 60.0%
CTCCGGTGGGATCAAAGCCA+TGG + chr4.4:41673580-41673599 None:intergenic 60.0%
CTGAAAGCGGAAGCGAGCTC+CGG + chr4.4:41673597-41673616 None:intergenic 60.0%
GAACCCGAGCGAGATGGATC+AGG - chr4.4:41673765-41673784 MS.gene021153:CDS 60.0%
GACGAAGAACCCGAGCGAGA+TGG - chr4.4:41673759-41673778 MS.gene021153:CDS 60.0%
GAGCTAGAACCACCCGAAGG+AGG + chr4.4:41674356-41674375 None:intergenic 60.0%
TGTGGGGTCCACACCAACCT+CGG + chr4.4:41673888-41673907 None:intergenic 60.0%
! AGAGAGTACGGAGAGGCTCC+GGG + chr4.4:41673626-41673645 None:intergenic 60.0%
AAAGCGGAAGCGAGCTCCGG+TGG + chr4.4:41673594-41673613 None:intergenic 65.0%
AAGCGGAAGCGAGCTCCGGT+GGG + chr4.4:41673593-41673612 None:intergenic 65.0%
! GAGAGTACGGAGAGGCTCCG+GGG + chr4.4:41673625-41673644 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41673492 41674472 41673492 ID=MS.gene021153
chr4.4 mRNA 41673492 41674472 41673492 ID=MS.gene021153.t1;Parent=MS.gene021153
chr4.4 exon 41673492 41674472 41673492 ID=MS.gene021153.t1.exon1;Parent=MS.gene021153.t1
chr4.4 CDS 41673492 41674472 41673492 ID=cds.MS.gene021153.t1;Parent=MS.gene021153.t1
Gene Sequence

>MS.gene021153

ATGGGTGAAGAGGTTCGAACAGACGAAGAACGTGTAACGGAGTGGGAAAAGGGTCTCCCTAACCTCCACGATCTCACTCCTCTTTCCATGGCTTTGATCCCACCGGAGCTCGCTTCCGCTTTCAGCATCTCCCCGGAGCCTCTCCGTACTCTCTTCGACGTCAACCGCGCCTCTCGCAACACACTCTCTCTTCTCCGAAGCAACTCCGGAACAATCACTCATCAAATCAATCAACCCGTGTCCAGCGAAGTAGAAGAAGTTGAAGAAGACGAAGAACCCGAGCGAGATGGATCAGGTTCGAATTCAAGAAAACATAGGAAAATAGATTCTGTTACAGAAGAAGTGGAAGCTGAAGCAGATTCGGCTGTTAGAACAACGGAGACGATAAAAAGGCCGAGGTTGGTGTGGACCCCACAGCTACATAAAAGATTTGTGGATGTTGTTGCTCATCTTGGGATAAAGAACGCGGTTCCGAAAACGATTATGCAGTTGATGAATGTTGAAGGATTAACAAGAGAAAATGTTGCTAGTCATTTGCAGAAATATCGTCTTTATTTGAAGAGAATGCAAGGTCTTTCAAATGATGCACCTTCTTCTTCTGATCATCTTTTTGCTTCTACACCTGTGCCGCAGAGTTTGCATGAAACCGGTTCTGCTAATCATTCTCATTCACATTCAAATGCTAATCATTCGAATGGAGGGCATGGAAATGGACATGGAAATTCGCATTCTCATTCACATTCGCATCATTCTGTGACGATTCCTATGCCTTATCCACCACCACCTTCGACTATGATGTCAATGCCGCCTATGATTGGTATGGGACATCATCCACATGGGCATATGGGGATGAGTTTGCCTCCTCCTTCGGGTGGTTCTAGCTCTTATCGCTCGCATCCATTTAATATGATGCATCATAGGGATTGGCCTCCGAATCCACATTCTCATCCTCATATGTCTTCCAATGATAGTAATAAATAA
Protein sequence

>MS.gene021153.t1

MGEEVRTDEERVTEWEKGLPNLHDLTPLSMALIPPELASAFSISPEPLRTLFDVNRASRNTLSLLRSNSGTITHQINQPVSSEVEEVEEDEEPERDGSGSNSRKHRKIDSVTEEVEAEADSAVRTTETIKRPRLVWTPQLHKRFVDVVAHLGIKNAVPKTIMQLMNVEGLTRENVASHLQKYRLYLKRMQGLSNDAPSSSDHLFASTPVPQSLHETGSANHSHSHSNANHSNGGHGNGHGNSHSHSHSHHSVTIPMPYPPPPSTMMSMPPMIGMGHHPHGHMGMSLPPPSGGSSSYRSHPFNMMHHRDWPPNPHSHPHMSSNDSNK