AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30562

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30562.t1 MTR_4g064730 89.634 328 16 2 1 326 1 312 4.33e-168 470
MS.gene30562.t1 MTR_7g089010 43.534 232 76 5 8 223 5 197 7.22e-43 150
MS.gene30562.t1 MTR_4g121020 66.176 68 23 0 126 193 106 173 4.47e-25 105
MS.gene30562.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.29e-25 105
MS.gene30562.t1 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.50e-25 105
MS.gene30562.t1 MTR_2g034960 49.057 106 47 1 126 224 206 311 3.33e-24 103
MS.gene30562.t1 MTR_7g117705 42.553 141 78 3 130 267 190 330 2.38e-23 100
MS.gene30562.t1 MTR_3g106220 49.485 97 46 1 130 226 197 290 3.56e-23 100
MS.gene30562.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.57e-22 98.6
MS.gene30562.t1 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.82e-22 98.6
MS.gene30562.t1 MTR_4g131570 68.852 61 19 0 128 188 200 260 1.70e-21 95.5
MS.gene30562.t1 MTR_1g032570 57.143 77 29 1 113 189 117 189 2.26e-21 95.1
MS.gene30562.t1 MTR_1g032570 57.143 77 29 1 113 189 186 258 3.15e-21 94.7
MS.gene30562.t1 MTR_1g013170 44.954 109 45 2 130 227 190 294 8.07e-21 93.2
MS.gene30562.t1 MTR_4g131580 68.852 61 19 0 128 188 197 257 1.59e-20 92.8
MS.gene30562.t1 MTR_8g079940 51.685 89 41 2 113 200 190 277 1.80e-19 89.4
MS.gene30562.t1 MTR_0450s0040 39.200 125 67 2 128 246 166 287 1.80e-19 88.2
MS.gene30562.t1 MTR_3g102600 58.333 60 25 0 130 189 82 141 3.90e-19 84.0
MS.gene30562.t1 MTR_1g013180 51.316 76 37 0 130 205 341 416 4.26e-19 88.6
MS.gene30562.t1 MTR_3g102600 58.333 60 25 0 130 189 131 190 5.17e-19 84.7
MS.gene30562.t1 MTR_7g026400 45.556 90 40 2 124 207 150 236 1.33e-18 87.0
MS.gene30562.t1 MTR_4g131600 57.812 64 27 0 126 189 196 259 2.28e-18 86.3
MS.gene30562.t1 MTR_3g086100 36.000 150 85 4 102 247 105 247 1.12e-17 84.0
MS.gene30562.t1 MTR_3g086100 36.000 150 85 4 102 247 176 318 1.21e-17 84.0
MS.gene30562.t1 MTR_4g021760 33.951 162 89 5 110 258 146 302 5.41e-17 80.5
MS.gene30562.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 2.46e-16 80.1
MS.gene30562.t1 MTR_4g098870 37.500 120 64 1 76 184 244 363 2.71e-16 79.7
MS.gene30562.t1 MTR_4g098870 37.500 120 64 1 76 184 283 402 2.94e-16 79.7
MS.gene30562.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 3.22e-16 79.7
MS.gene30562.t1 MTR_4g098870 37.500 120 64 1 76 184 264 383 3.55e-16 79.7
MS.gene30562.t1 MTR_3g102590 51.389 72 33 1 130 199 142 213 3.86e-16 77.0
MS.gene30562.t1 MTR_5g014040 53.333 60 28 0 130 189 310 369 2.79e-15 77.0
MS.gene30562.t1 MTR_2g090675 43.038 79 43 1 130 206 160 238 2.16e-14 73.9
MS.gene30562.t1 MTR_4g086835 49.206 63 32 0 127 189 270 332 3.16e-14 73.2
MS.gene30562.t1 MTR_4g078810 44.928 69 38 0 130 198 157 225 3.39e-14 73.2
MS.gene30562.t1 MTR_4g021790 47.826 69 36 0 120 188 157 225 7.57e-14 70.9
MS.gene30562.t1 MTR_5g014040 49.231 65 28 1 130 189 270 334 2.68e-13 70.9
MS.gene30562.t1 MTR_5g041350 38.679 106 53 4 128 227 12 111 2.80e-13 70.1
MS.gene30562.t1 MTR_5g014040 49.231 65 28 1 130 189 310 374 3.09e-13 70.5
MS.gene30562.t1 MTR_3g102600 50.943 53 26 0 130 182 82 134 3.16e-13 66.6
MS.gene30562.t1 MTR_3g102600 50.943 53 26 0 130 182 131 183 3.46e-13 67.4
MS.gene30562.t1 MTR_5g017980 48.333 60 31 0 130 189 191 250 5.09e-13 69.3
MS.gene30562.t1 MTR_5g017980 48.333 60 31 0 130 189 192 251 5.27e-13 68.9
MS.gene30562.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 5.63e-13 69.3
MS.gene30562.t1 MTR_4g086835 48.438 64 32 1 127 189 270 333 6.69e-13 69.3
MS.gene30562.t1 MTR_6g444980 52.174 69 31 2 117 184 22 89 6.77e-13 69.3
MS.gene30562.t1 MTR_1g090670 60.000 55 21 1 131 184 44 98 7.49e-13 68.6
MS.gene30562.t1 MTR_4g113140 40.404 99 52 3 119 210 218 316 8.63e-13 68.9
MS.gene30562.t1 MTR_7g098250 34.127 126 76 3 65 183 190 315 8.84e-13 69.3
MS.gene30562.t1 MTR_8g105600 56.140 57 25 0 134 190 190 246 8.95e-13 69.3
MS.gene30562.t1 MTR_2g027800 52.055 73 29 2 131 197 39 111 9.90e-13 68.2
MS.gene30562.t1 MTR_2g027800 60.000 55 21 1 131 184 39 93 1.30e-12 67.8
MS.gene30562.t1 MTR_2g086450 55.172 58 25 1 128 184 42 99 1.67e-12 68.2
MS.gene30562.t1 MTR_1g093080 43.243 74 41 1 127 199 207 280 1.86e-12 67.8
MS.gene30562.t1 MTR_4g021855 49.231 65 32 1 125 188 161 225 2.55e-12 67.0
MS.gene30562.t1 MTR_1g080330 50.685 73 34 2 112 183 157 228 2.80e-12 66.6
MS.gene30562.t1 MTR_1g080330 49.315 73 35 2 112 183 157 228 2.83e-12 67.4
MS.gene30562.t1 MTR_6g032990 47.222 72 36 2 128 198 39 109 3.41e-12 67.0
MS.gene30562.t1 MTR_7g069660 49.315 73 36 1 112 183 52 124 4.59e-12 66.6
MS.gene30562.t1 MTR_6g045327 48.276 58 30 0 130 187 171 228 5.89e-12 66.2
MS.gene30562.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 5.94e-12 65.9
MS.gene30562.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 8.40e-12 65.5
MS.gene30562.t1 MTR_2g027860 48.611 72 35 2 131 201 49 119 8.91e-12 65.5
MS.gene30562.t1 MTR_5g027440 54.545 55 24 1 131 184 25 79 9.21e-12 65.1
MS.gene30562.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 9.68e-12 65.9
MS.gene30562.t1 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.47e-11 65.1
MS.gene30562.t1 MTR_5g017980 47.541 61 31 1 130 189 192 252 1.48e-11 64.7
MS.gene30562.t1 MTR_5g054300 42.105 76 43 1 130 204 197 272 1.54e-11 65.1
MS.gene30562.t1 MTR_1g053830 50.943 53 25 1 133 184 20 72 2.11e-11 60.1
MS.gene30562.t1 MTR_6g453220 63.830 47 17 0 148 194 380 426 3.51e-11 64.3
MS.gene30562.t1 MTR_0223s0040 49.231 65 32 1 120 183 7 71 3.79e-11 63.2
MS.gene30562.t1 MTR_3g102590 54.902 51 23 0 130 180 142 192 5.35e-11 61.6
MS.gene30562.t1 MTR_1g112370 50.794 63 30 1 122 183 13 75 5.37e-11 63.2
MS.gene30562.t1 MTR_4g081710 50.000 58 28 1 128 184 14 71 5.55e-11 63.2
MS.gene30562.t1 MTR_2g023580 48.148 81 39 3 105 183 29 108 7.07e-11 62.0
MS.gene30562.t1 MTR_2g084230 53.125 64 29 1 121 183 87 150 9.49e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30562.t1 AT3G46640 57.021 235 75 4 1 215 1 229 5.32e-78 241
MS.gene30562.t1 AT3G46640 57.021 235 75 4 1 215 1 229 5.32e-78 241
MS.gene30562.t1 AT3G46640 57.021 235 75 4 1 215 1 229 2.17e-77 240
MS.gene30562.t1 AT5G59570 54.331 254 91 8 11 250 25 267 3.85e-73 228
MS.gene30562.t1 AT5G59570 54.331 254 91 8 11 250 25 267 3.85e-73 228
MS.gene30562.t1 AT3G10760 47.596 208 80 5 8 210 5 188 3.40e-39 141
MS.gene30562.t1 AT5G05090 81.176 85 15 1 127 210 77 161 3.14e-38 137
MS.gene30562.t1 AT2G40970 72.340 94 17 1 126 210 100 193 4.39e-38 136
MS.gene30562.t1 AT3G16857 52.128 94 40 1 100 193 141 229 2.21e-24 103
MS.gene30562.t1 AT3G16857 52.128 94 40 1 100 193 211 299 2.52e-24 103
MS.gene30562.t1 AT3G16857 52.128 94 40 1 100 193 211 299 2.79e-24 103
MS.gene30562.t1 AT2G25180 39.264 163 78 4 32 194 117 258 4.04e-22 97.4
MS.gene30562.t1 AT2G01760 57.895 76 29 1 130 202 134 209 7.49e-22 94.4
MS.gene30562.t1 AT5G58080 55.696 79 30 1 128 201 191 269 1.14e-21 96.3
MS.gene30562.t1 AT2G01760 57.895 76 29 1 130 202 199 274 2.14e-21 94.0
MS.gene30562.t1 AT2G01760 57.895 76 29 1 130 202 227 302 3.59e-21 93.6
MS.gene30562.t1 AT4G31920 44.792 96 50 1 130 225 182 274 1.35e-18 87.0
MS.gene30562.t1 AT2G20570 33.544 158 99 2 41 196 65 218 1.81e-16 80.1
MS.gene30562.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.00e-16 79.7
MS.gene30562.t1 AT5G44190 50.000 74 35 1 130 203 146 217 2.18e-16 79.3
MS.gene30562.t1 AT5G07210 46.053 76 39 1 119 192 210 285 7.52e-16 78.6
MS.gene30562.t1 AT5G07210 46.053 76 39 1 119 192 218 293 7.58e-16 78.6
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 2.16e-15 77.0
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 2.16e-15 77.0
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 2.16e-15 77.0
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 2.16e-15 77.0
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 2.16e-15 77.0
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 2.16e-15 77.0
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.51e-15 76.6
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.51e-15 76.6
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.51e-15 76.6
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.51e-15 76.6
MS.gene30562.t1 AT4G18020 30.275 218 120 6 84 293 242 435 3.51e-15 76.6
MS.gene30562.t1 AT2G20570 36.885 122 68 2 80 196 117 234 5.00e-15 75.9
MS.gene30562.t1 AT1G67710 42.857 98 49 3 97 190 94 188 6.29e-15 75.5
MS.gene30562.t1 AT1G67710 42.857 98 49 3 97 190 85 179 6.50e-15 75.5
MS.gene30562.t1 AT1G67710 42.857 98 49 3 97 190 159 253 1.03e-14 75.1
MS.gene30562.t1 AT3G12730 52.055 73 33 2 113 184 6 77 1.90e-14 72.0
MS.gene30562.t1 AT2G27070 45.679 81 41 2 130 207 224 304 2.66e-14 73.9
MS.gene30562.t1 AT3G04030 49.275 69 34 1 128 196 42 109 8.28e-14 72.0
MS.gene30562.t1 AT1G79430 53.623 69 30 2 117 184 21 88 1.10e-13 71.6
MS.gene30562.t1 AT5G45580 56.364 55 23 1 131 184 7 61 4.41e-13 68.6
MS.gene30562.t1 AT3G04030 50.000 70 33 2 128 196 42 110 4.66e-13 69.7
MS.gene30562.t1 AT5G45580 56.364 55 23 1 131 184 23 77 5.33e-13 68.6
MS.gene30562.t1 AT3G04030 50.000 70 33 2 128 196 42 110 5.37e-13 69.7
MS.gene30562.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.39e-13 69.3
MS.gene30562.t1 AT5G18240 51.429 70 32 2 128 196 42 110 6.39e-13 69.3
MS.gene30562.t1 AT3G24120 58.182 55 22 1 131 184 41 95 6.94e-13 68.6
MS.gene30562.t1 AT3G24120 58.182 55 22 1 131 184 41 95 7.05e-13 68.6
MS.gene30562.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.23e-13 69.3
MS.gene30562.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.55e-13 69.3
MS.gene30562.t1 AT5G18240 51.429 70 32 2 128 196 42 110 7.55e-13 69.3
MS.gene30562.t1 AT4G13640 58.182 55 22 1 131 184 37 91 8.08e-13 68.2
MS.gene30562.t1 AT1G13300 42.529 87 46 3 130 212 180 266 8.37e-13 68.6
MS.gene30562.t1 AT4G13640 58.182 55 22 1 131 184 37 91 8.54e-13 68.2
MS.gene30562.t1 AT3G62670 55.932 59 21 2 130 183 210 268 8.94e-13 68.9
MS.gene30562.t1 AT2G03500 47.692 65 33 1 125 188 227 291 1.18e-12 68.6
MS.gene30562.t1 AT2G01060 45.055 91 45 3 118 203 2 92 1.76e-12 67.4
MS.gene30562.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.13e-11 65.9
MS.gene30562.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.13e-11 65.9
MS.gene30562.t1 AT3G13040 55.357 56 24 1 130 184 240 295 1.13e-11 65.9
MS.gene30562.t1 AT2G38300 40.196 102 57 2 107 207 30 128 1.25e-11 65.1
MS.gene30562.t1 AT1G49560 44.118 68 37 1 122 188 183 250 2.16e-11 64.3
MS.gene30562.t1 AT1G25550 48.333 60 30 1 130 188 208 267 3.62e-11 63.9
MS.gene30562.t1 AT5G49240 50.980 51 25 0 130 180 222 272 4.90e-11 63.2
MS.gene30562.t1 AT3G25790 40.449 89 49 3 135 219 200 288 6.95e-11 63.2

Find 92 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGATGCATCATATTAAA+TGG 0.267208 4.2:+40546782 None:intergenic
GGAGTGAGATCGTGGAGGTT+AGG 0.288087 4.2:+40547622 None:intergenic
CATTTAATATGATGCATCAT+AGG 0.291545 4.2:-40546781 MS.gene30562:CDS
GATGAGTTTGCCTCCTCCTT+CGG 0.309025 4.2:-40546831 MS.gene30562:CDS
GAGTGAGATCGTGGAGGTTA+GGG 0.316953 4.2:+40547623 None:intergenic
ACATCATCCACATGGGCATA+TGG 0.344716 4.2:-40546855 MS.gene30562:CDS
GTGGATGTTGTTGCTCATCT+TGG 0.347586 4.2:-40547247 MS.gene30562:CDS
ATGAGTTTGCCTCCTCCTTC+GGG 0.352579 4.2:-40546830 MS.gene30562:CDS
GAACAACGGAGACGATAAAA+AGG 0.405625 4.2:-40547309 MS.gene30562:CDS
GTCTTCTACTTCGCTGGACA+CGG 0.409549 4.2:+40547444 None:intergenic
CATCATCCACATGGGCATAT+GGG 0.413116 4.2:-40546854 MS.gene30562:CDS
AAGAGAGTACGGAGAGGCTC+CGG 0.421773 4.2:+40547547 None:intergenic
GTCGAAGGTGGTGGTGGATA+AGG 0.424874 4.2:+40546911 None:intergenic
TCAAATGCTAATCATTCAAA+TGG 0.429533 4.2:-40547004 MS.gene30562:CDS
TTCAACGTCTTCTACTTCGC+TGG 0.432464 4.2:+40547438 None:intergenic
TGATTGTTCCGGAGTTGCTT+CGG 0.434817 4.2:+40547485 None:intergenic
TGGATGTTGTTGCTCATCTT+GGG 0.442275 4.2:-40547246 MS.gene30562:CDS
CCGCAGAGTTTGCATGAAAC+CGG 0.443486 4.2:-40547052 MS.gene30562:CDS
TTCCATGGCTTTGATCCCAC+CGG 0.445349 4.2:-40547596 MS.gene30562:CDS
AGTGGAAGCTGAAGCAGATT+CGG 0.449529 4.2:-40547338 MS.gene30562:CDS
ACGTGTAACGGAGTGGGAAA+AGG 0.453107 4.2:-40547650 MS.gene30562:CDS
AGAGAGTACGGAGAGGCTCC+GGG 0.454039 4.2:+40547548 None:intergenic
GAACCCGAGCGAGATGGATC+AGG 0.456693 4.2:-40547406 MS.gene30562:CDS
CTGAAAGCGGAAGCGAGCTC+CGG 0.460721 4.2:+40547577 None:intergenic
TGATGTCCCATACCAATCAT+AGG 0.460751 4.2:+40546872 None:intergenic
TCATGCAAACTCTGCGGCAC+AGG 0.465750 4.2:+40547058 None:intergenic
GATTTGATGAGTGATTGTTC+CGG 0.471479 4.2:+40547474 None:intergenic
ACAGCTACATAAAAGATTTG+TGG 0.472539 4.2:-40547266 MS.gene30562:CDS
TCAAATGGAGGGCATGGTAA+TGG 0.474640 4.2:-40546989 MS.gene30562:CDS
CGATCTCACTCCTCTTTCCA+TGG 0.482732 4.2:-40547611 MS.gene30562:CDS
ATATGATGCATCATAGGGAT+TGG 0.484646 4.2:-40546775 MS.gene30562:CDS
ATGAGAATGATTAGCAGAAC+CGG 0.486272 4.2:+40547033 None:intergenic
ATTTAATATGATGCATCATA+GGG 0.488329 4.2:-40546780 MS.gene30562:CDS
ATGCAGTTGATGAATGTTGA+AGG 0.492393 4.2:-40547196 MS.gene30562:CDS
TTCCGCTTTCAGCATCTCCC+CGG 0.493661 4.2:-40547566 MS.gene30562:CDS
ATCATAGTCGAAGGTGGTGG+TGG 0.494086 4.2:+40546905 None:intergenic
AGATTCTGTTACAGAGGAAG+TGG 0.499261 4.2:-40547356 MS.gene30562:CDS
CGTGTAACGGAGTGGGAAAA+GGG 0.517852 4.2:-40547649 MS.gene30562:CDS
TCTCTTCTCCGAAGCAACTC+CGG 0.518098 4.2:-40547493 MS.gene30562:CDS
CGAGCGAGATGGATCAGGTT+CGG 0.519486 4.2:-40547401 MS.gene30562:CDS
AAAGCGGAAGCGAGCTCCGG+TGG 0.521066 4.2:+40547580 None:intergenic
GACGAAGAACCCGAGCGAGA+TGG 0.525147 4.2:-40547412 MS.gene30562:CDS
CGAACCTGATCCATCTCGCT+CGG 0.534136 4.2:+40547402 None:intergenic
TGAGGATGAGAATGTGGATT+CGG 0.534692 4.2:+40546749 None:intergenic
AAAAGATGATCAGAAGAAGA+AGG 0.541009 4.2:+40547091 None:intergenic
GAACCTGATCCATCTCGCTC+GGG 0.541572 4.2:+40547403 None:intergenic
ATGTCAATGCCGCCTATGAT+TGG 0.542928 4.2:-40546884 MS.gene30562:CDS
TCTTCTACTTCGCTGGACAC+GGG 0.550393 4.2:+40547445 None:intergenic
ACGAAGAACGTGTAACGGAG+TGG 0.550873 4.2:-40547657 MS.gene30562:CDS
GACATGTGAGGATGAGAATG+TGG 0.553221 4.2:+40546743 None:intergenic
AATGCTAATCATTCAAATGG+AGG 0.554348 4.2:-40547001 MS.gene30562:CDS
ATCATCCACATGGGCATATG+GGG 0.557841 4.2:-40546853 MS.gene30562:CDS
CTAGAACCACCCGAAGGAGG+AGG 0.558990 4.2:+40546821 None:intergenic
GGGATCAAAGCCATGGAAAG+AGG 0.559832 4.2:+40547601 None:intergenic
ATGCCGCCTATGATTGGTAT+GGG 0.560585 4.2:-40546878 MS.gene30562:CDS
GGTTGACGTCGAAGAGAGTA+CGG 0.568518 4.2:+40547536 None:intergenic
CTCCGGTGGGATCAAAGCCA+TGG 0.569390 4.2:+40547594 None:intergenic
AATCATTCAAATGGAGGGCA+TGG 0.569625 4.2:-40546995 MS.gene30562:CDS
GGAGGGCATGGTAATGGACA+TGG 0.571823 4.2:-40546983 MS.gene30562:CDS
AATGCCGCCTATGATTGGTA+TGG 0.579585 4.2:-40546879 MS.gene30562:CDS
ATGCTAATCATTCAAATGGA+GGG 0.580774 4.2:-40547000 MS.gene30562:CDS
CTTTATTTGAAGAGAATGCA+AGG 0.583827 4.2:-40547130 MS.gene30562:CDS
GACATCATAGTCGAAGGTGG+TGG 0.587152 4.2:+40546902 None:intergenic
AACAAACTGAATGGGTGAAG+AGG 0.590082 4.2:-40547689 None:intergenic
GGTGGTGGTGGATAAGGCAT+AGG 0.595338 4.2:+40546917 None:intergenic
CTCCGGGGAGATGCTGAAAG+CGG 0.595726 4.2:+40547564 None:intergenic
TGGAAAGAGGAGTGAGATCG+TGG 0.601806 4.2:+40547614 None:intergenic
TTTATTTGAAGAGAATGCAA+GGG 0.602218 4.2:-40547129 MS.gene30562:CDS
GGCATTGACATCATAGTCGA+AGG 0.602892 4.2:+40546896 None:intergenic
GAGGGCATGGTAATGGACAT+GGG 0.610778 4.2:-40546982 MS.gene30562:CDS
AAGAGAGAGTGTGTTGCGAG+AGG 0.612371 4.2:+40547510 None:intergenic
GAGCTAGAACCACCCGAAGG+AGG 0.617421 4.2:+40546818 None:intergenic
CTCATCCCCATATGCCCATG+TGG 0.624417 4.2:+40546848 None:intergenic
GAGAGTACGGAGAGGCTCCG+GGG 0.626074 4.2:+40547549 None:intergenic
AGTTTGCCTCCTCCTTCGGG+TGG 0.628086 4.2:-40546827 MS.gene30562:CDS
CGAAGAACGTGTAACGGAGT+GGG 0.629837 4.2:-40547656 MS.gene30562:CDS
TGTGGGGTCCACACCAACCT+CGG 0.635154 4.2:+40547286 None:intergenic
AAGCGGAAGCGAGCTCCGGT+GGG 0.636737 4.2:+40547581 None:intergenic
AACAGACGAAGAACGTGTAA+CGG 0.639303 4.2:-40547662 MS.gene30562:CDS
GGATGAGAATGTGGATTCGG+AGG 0.643753 4.2:+40546752 None:intergenic
GGTATGGGACATCATCCACA+TGG 0.649470 4.2:-40546863 MS.gene30562:CDS
AAAGAGGAGTGAGATCGTGG+AGG 0.675435 4.2:+40547617 None:intergenic
ATTGACATCATAGTCGAAGG+TGG 0.681193 4.2:+40546899 None:intergenic
AGAGTGTGTTGCGAGAGGCG+CGG 0.693296 4.2:+40547515 None:intergenic
CTATCATTGGAAGACATGTG+AGG 0.702805 4.2:+40546731 None:intergenic
TAAGAGCTAGAACCACCCGA+AGG 0.715458 4.2:+40546815 None:intergenic
AGATTCGGCTGTTAGAACAA+CGG 0.719098 4.2:-40547323 MS.gene30562:CDS
TCATCTTGGGATAAAGAACG+CGG 0.719383 4.2:-40547233 MS.gene30562:CDS
GTATGGGACATCATCCACAT+GGG 0.727717 4.2:-40546862 MS.gene30562:CDS
CCGGTTTCATGCAAACTCTG+CGG 0.740073 4.2:+40547052 None:intergenic
TGTCCCATACCAATCATAGG+CGG 0.741457 4.2:+40546875 None:intergenic
ACGTCGAAGAGAGTACGGAG+AGG 0.748301 4.2:+40547541 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATGATGCATCATA+GGG - chr4.2:40547620-40547639 MS.gene30562:CDS 20.0%
! AAAAATAGATTCTGTTACAG+AGG - chr4.2:40547038-40547057 MS.gene30562:CDS 25.0%
! CATTTAATATGATGCATCAT+AGG - chr4.2:40547619-40547638 MS.gene30562:CDS 25.0%
! CTATGATGCATCATATTAAA+TGG + chr4.2:40547621-40547640 None:intergenic 25.0%
! TCAAATGCTAATCATTCAAA+TGG - chr4.2:40547396-40547415 MS.gene30562:CDS 25.0%
! TTTATTTGAAGAGAATGCAA+GGG - chr4.2:40547271-40547290 MS.gene30562:CDS 25.0%
AAAAGATGATCAGAAGAAGA+AGG + chr4.2:40547312-40547331 None:intergenic 30.0%
AATGCTAATCATTCAAATGG+AGG - chr4.2:40547399-40547418 MS.gene30562:CDS 30.0%
ACAGCTACATAAAAGATTTG+TGG - chr4.2:40547134-40547153 MS.gene30562:CDS 30.0%
ATGCTAATCATTCAAATGGA+GGG - chr4.2:40547400-40547419 MS.gene30562:CDS 30.0%
! AAATCTTTTATGTAGCTGTG+GGG + chr4.2:40547133-40547152 None:intergenic 30.0%
! ACAAATCTTTTATGTAGCTG+TGG + chr4.2:40547135-40547154 None:intergenic 30.0%
! ATCAACTGCATAATCGTTTT+CGG + chr4.2:40547195-40547214 None:intergenic 30.0%
! CAAATCTTTTATGTAGCTGT+GGG + chr4.2:40547134-40547153 None:intergenic 30.0%
! CTTTATTTGAAGAGAATGCA+AGG - chr4.2:40547270-40547289 MS.gene30562:CDS 30.0%
ATATGATGCATCATAGGGAT+TGG - chr4.2:40547625-40547644 MS.gene30562:CDS 35.0%
ATGAGAATGATTAGCAGAAC+CGG + chr4.2:40547370-40547389 None:intergenic 35.0%
GATTTGATGAGTGATTGTTC+CGG + chr4.2:40546929-40546948 None:intergenic 35.0%
!! ATGCAGTTGATGAATGTTGA+AGG - chr4.2:40547204-40547223 MS.gene30562:CDS 35.0%
AACAGACGAAGAACGTGTAA+CGG - chr4.2:40546738-40546757 MS.gene30562:CDS 40.0%
AATCATTCAAATGGAGGGCA+TGG - chr4.2:40547405-40547424 MS.gene30562:CDS 40.0%
AGATTCGGCTGTTAGAACAA+CGG - chr4.2:40547077-40547096 MS.gene30562:CDS 40.0%
AGATTCTGTTACAGAGGAAG+TGG - chr4.2:40547044-40547063 MS.gene30562:CDS 40.0%
ATTGACATCATAGTCGAAGG+TGG + chr4.2:40547504-40547523 None:intergenic 40.0%
CTATCATTGGAAGACATGTG+AGG + chr4.2:40547672-40547691 None:intergenic 40.0%
GAACAACGGAGACGATAAAA+AGG - chr4.2:40547091-40547110 MS.gene30562:CDS 40.0%
TCATCTTGGGATAAAGAACG+CGG - chr4.2:40547167-40547186 MS.gene30562:CDS 40.0%
TGAGGATGAGAATGTGGATT+CGG + chr4.2:40547654-40547673 None:intergenic 40.0%
TGATGTCCCATACCAATCAT+AGG + chr4.2:40547531-40547550 None:intergenic 40.0%
TGGATGTTGTTGCTCATCTT+GGG - chr4.2:40547154-40547173 MS.gene30562:CDS 40.0%
AATGCCGCCTATGATTGGTA+TGG - chr4.2:40547521-40547540 MS.gene30562:CDS 45.0%
ACATCATCCACATGGGCATA+TGG - chr4.2:40547545-40547564 MS.gene30562:CDS 45.0%
AGTGGAAGCTGAAGCAGATT+CGG - chr4.2:40547062-40547081 MS.gene30562:CDS 45.0%
ATCATCCACATGGGCATATG+GGG - chr4.2:40547547-40547566 MS.gene30562:CDS 45.0%
ATGCCGCCTATGATTGGTAT+GGG - chr4.2:40547522-40547541 MS.gene30562:CDS 45.0%
ATGTCAATGCCGCCTATGAT+TGG - chr4.2:40547516-40547535 MS.gene30562:CDS 45.0%
CATCATCCACATGGGCATAT+GGG - chr4.2:40547546-40547565 MS.gene30562:CDS 45.0%
GACATGTGAGGATGAGAATG+TGG + chr4.2:40547660-40547679 None:intergenic 45.0%
GTATGGGACATCATCCACAT+GGG - chr4.2:40547538-40547557 MS.gene30562:CDS 45.0%
GTGGATGTTGTTGCTCATCT+TGG - chr4.2:40547153-40547172 MS.gene30562:CDS 45.0%
TGATTGTTCCGGAGTTGCTT+CGG + chr4.2:40546918-40546937 None:intergenic 45.0%
TGTCCCATACCAATCATAGG+CGG + chr4.2:40547528-40547547 None:intergenic 45.0%
TTCAACGTCTTCTACTTCGC+TGG + chr4.2:40546965-40546984 None:intergenic 45.0%
! TCAAATGGAGGGCATGGTAA+TGG - chr4.2:40547411-40547430 MS.gene30562:CDS 45.0%
!! GGCATTGACATCATAGTCGA+AGG + chr4.2:40547507-40547526 None:intergenic 45.0%
AAAGAGGAGTGAGATCGTGG+AGG + chr4.2:40546786-40546805 None:intergenic 50.0%
ATGAGTTTGCCTCCTCCTTC+GGG - chr4.2:40547570-40547589 MS.gene30562:CDS 50.0%
CCGCAGAGTTTGCATGAAAC+CGG - chr4.2:40547348-40547367 MS.gene30562:CDS 50.0%
CCGGTTTCATGCAAACTCTG+CGG + chr4.2:40547351-40547370 None:intergenic 50.0%
CGATCTCACTCCTCTTTCCA+TGG - chr4.2:40546789-40546808 MS.gene30562:CDS 50.0%
GACATCATAGTCGAAGGTGG+TGG + chr4.2:40547501-40547520 None:intergenic 50.0%
GACGATAAAAAGGCCGAGGT+TGG - chr4.2:40547101-40547120 MS.gene30562:CDS 50.0%
GAGTGAGATCGTGGAGGTTA+GGG + chr4.2:40546780-40546799 None:intergenic 50.0%
GATGAGTTTGCCTCCTCCTT+CGG - chr4.2:40547569-40547588 MS.gene30562:CDS 50.0%
GGATGAGAATGTGGATTCGG+AGG + chr4.2:40547651-40547670 None:intergenic 50.0%
GGGATCAAAGCCATGGAAAG+AGG + chr4.2:40546802-40546821 None:intergenic 50.0%
GGTATGGGACATCATCCACA+TGG - chr4.2:40547537-40547556 MS.gene30562:CDS 50.0%
GGTTGACGTCGAAGAGAGTA+CGG + chr4.2:40546867-40546886 None:intergenic 50.0%
GTCTTCTACTTCGCTGGACA+CGG + chr4.2:40546959-40546978 None:intergenic 50.0%
TAAGAGCTAGAACCACCCGA+AGG + chr4.2:40547588-40547607 None:intergenic 50.0%
TCTCTTCTCCGAAGCAACTC+CGG - chr4.2:40546907-40546926 MS.gene30562:CDS 50.0%
TCTTCTACTTCGCTGGACAC+GGG + chr4.2:40546958-40546977 None:intergenic 50.0%
TGGAAAGAGGAGTGAGATCG+TGG + chr4.2:40546789-40546808 None:intergenic 50.0%
! AAGAGAGAGTGTGTTGCGAG+AGG + chr4.2:40546893-40546912 None:intergenic 50.0%
! ACGAAGAACGTGTAACGGAG+TGG - chr4.2:40546743-40546762 MS.gene30562:CDS 50.0%
! ACGTGTAACGGAGTGGGAAA+AGG - chr4.2:40546750-40546769 MS.gene30562:CDS 50.0%
! ATCATAGTCGAAGGTGGTGG+TGG + chr4.2:40547498-40547517 None:intergenic 50.0%
! CGAAGAACGTGTAACGGAGT+GGG - chr4.2:40546744-40546763 MS.gene30562:CDS 50.0%
! CGTGTAACGGAGTGGGAAAA+GGG - chr4.2:40546751-40546770 MS.gene30562:CDS 50.0%
!! GAGGGCATGGTAATGGACAT+GGG - chr4.2:40547418-40547437 MS.gene30562:CDS 50.0%
!! TAAAAAGGCCGAGGTTGGTG+TGG - chr4.2:40547106-40547125 MS.gene30562:CDS 50.0%
!! TTCCATGGCTTTGATCCCAC+CGG - chr4.2:40546804-40546823 MS.gene30562:CDS 50.0%
CGAACCTGATCCATCTCGCT+CGG + chr4.2:40547001-40547020 None:intergenic 55.0%
CGAGCGAGATGGATCAGGTT+CGG - chr4.2:40546999-40547018 MS.gene30562:CDS 55.0%
CGGAGACGATAAAAAGGCCG+AGG - chr4.2:40547097-40547116 MS.gene30562:CDS 55.0%
CTCATCCCCATATGCCCATG+TGG + chr4.2:40547555-40547574 None:intergenic 55.0%
GAACCTGATCCATCTCGCTC+GGG + chr4.2:40547000-40547019 None:intergenic 55.0%
GGAGTGAGATCGTGGAGGTT+AGG + chr4.2:40546781-40546800 None:intergenic 55.0%
TCATGCAAACTCTGCGGCAC+AGG + chr4.2:40547345-40547364 None:intergenic 55.0%
TTCCGCTTTCAGCATCTCCC+CGG - chr4.2:40546834-40546853 MS.gene30562:CDS 55.0%
! AAGAGAGTACGGAGAGGCTC+CGG + chr4.2:40546856-40546875 None:intergenic 55.0%
! ACGTCGAAGAGAGTACGGAG+AGG + chr4.2:40546862-40546881 None:intergenic 55.0%
!! GGAGGGCATGGTAATGGACA+TGG - chr4.2:40547417-40547436 MS.gene30562:CDS 55.0%
!! GGTGGTGGTGGATAAGGCAT+AGG + chr4.2:40547486-40547505 None:intergenic 55.0%
!! GTCGAAGGTGGTGGTGGATA+AGG + chr4.2:40547492-40547511 None:intergenic 55.0%
AGAGTGTGTTGCGAGAGGCG+CGG + chr4.2:40546888-40546907 None:intergenic 60.0%
AGTTTGCCTCCTCCTTCGGG+TGG - chr4.2:40547573-40547592 MS.gene30562:CDS 60.0%
CTAGAACCACCCGAAGGAGG+AGG + chr4.2:40547582-40547601 None:intergenic 60.0%
CTCCGGGGAGATGCTGAAAG+CGG + chr4.2:40546839-40546858 None:intergenic 60.0%
CTCCGGTGGGATCAAAGCCA+TGG + chr4.2:40546809-40546828 None:intergenic 60.0%
CTGAAAGCGGAAGCGAGCTC+CGG + chr4.2:40546826-40546845 None:intergenic 60.0%
GAACCCGAGCGAGATGGATC+AGG - chr4.2:40546994-40547013 MS.gene30562:CDS 60.0%
GACGAAGAACCCGAGCGAGA+TGG - chr4.2:40546988-40547007 MS.gene30562:CDS 60.0%
GAGCTAGAACCACCCGAAGG+AGG + chr4.2:40547585-40547604 None:intergenic 60.0%
TGTGGGGTCCACACCAACCT+CGG + chr4.2:40547117-40547136 None:intergenic 60.0%
! AGAGAGTACGGAGAGGCTCC+GGG + chr4.2:40546855-40546874 None:intergenic 60.0%
AAAGCGGAAGCGAGCTCCGG+TGG + chr4.2:40546823-40546842 None:intergenic 65.0%
AAGCGGAAGCGAGCTCCGGT+GGG + chr4.2:40546822-40546841 None:intergenic 65.0%
! GAGAGTACGGAGAGGCTCCG+GGG + chr4.2:40546854-40546873 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 40546721 40547701 40546721 ID=MS.gene30562
chr4.2 mRNA 40546721 40547701 40546721 ID=MS.gene30562.t1;Parent=MS.gene30562
chr4.2 exon 40546721 40547701 40546721 ID=MS.gene30562.t1.exon1;Parent=MS.gene30562.t1
chr4.2 CDS 40546721 40547701 40546721 ID=cds.MS.gene30562.t1;Parent=MS.gene30562.t1
Gene Sequence

>MS.gene30562

ATGGGTGAAGAGGTTCGAACAGACGAAGAACGTGTAACGGAGTGGGAAAAGGGTCTCCCTAACCTCCACGATCTCACTCCTCTTTCCATGGCTTTGATCCCACCGGAGCTCGCTTCCGCTTTCAGCATCTCCCCGGAGCCTCTCCGTACTCTCTTCGACGTCAACCGCGCCTCTCGCAACACACTCTCTCTTCTCCGAAGCAACTCCGGAACAATCACTCATCAAATCAATCAACCCGTGTCCAGCGAAGTAGAAGACGTTGAAGAAGACGAAGAACCCGAGCGAGATGGATCAGGTTCGGATTCAAGAAAACATCGAAAAATAGATTCTGTTACAGAGGAAGTGGAAGCTGAAGCAGATTCGGCTGTTAGAACAACGGAGACGATAAAAAGGCCGAGGTTGGTGTGGACCCCACAGCTACATAAAAGATTTGTGGATGTTGTTGCTCATCTTGGGATAAAGAACGCGGTTCCGAAAACGATTATGCAGTTGATGAATGTTGAAGGATTAACAAGAGAAAATGTTGCTAGTCATTTGCAGAAATATCGTCTTTATTTGAAGAGAATGCAAGGGCTTTCAAATGATGCACCTTCTTCTTCTGATCATCTTTTTGCTTCTACACCTGTGCCGCAGAGTTTGCATGAAACCGGTTCTGCTAATCATTCTCATTCACATTCAAATGCTAATCATTCAAATGGAGGGCATGGTAATGGACATGGGAATTCGCATTCTCATTCACATTCGCATCATTCTGTGACGATTCCTATGCCTTATCCACCACCACCTTCGACTATGATGTCAATGCCGCCTATGATTGGTATGGGACATCATCCACATGGGCATATGGGGATGAGTTTGCCTCCTCCTTCGGGTGGTTCTAGCTCTTATCGCTCGCATCCATTTAATATGATGCATCATAGGGATTGGCCTCCGAATCCACATTCTCATCCTCACATGTCTTCCAATGATAGTAATAAATAA
Protein sequence

>MS.gene30562.t1

MGEEVRTDEERVTEWEKGLPNLHDLTPLSMALIPPELASAFSISPEPLRTLFDVNRASRNTLSLLRSNSGTITHQINQPVSSEVEDVEEDEEPERDGSGSDSRKHRKIDSVTEEVEAEADSAVRTTETIKRPRLVWTPQLHKRFVDVVAHLGIKNAVPKTIMQLMNVEGLTRENVASHLQKYRLYLKRMQGLSNDAPSSSDHLFASTPVPQSLHETGSANHSHSHSNANHSNGGHGNGHGNSHSHSHSHHSVTIPMPYPPPPSTMMSMPPMIGMGHHPHGHMGMSLPPPSGGSSSYRSHPFNMMHHRDWPPNPHSHPHMSSNDSNK