AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004050.t1 MTR_4g063780 94.955 337 13 2 1 336 1 334 0.0 584
MS.gene004050.t1 MTR_2g096740 41.389 360 130 17 1 336 1 303 4.20e-63 202
MS.gene004050.t1 MTR_7g059400 44.144 333 129 10 1 312 1 297 1.53e-61 200
MS.gene004050.t1 MTR_2g096740 38.611 360 125 15 1 336 1 288 1.11e-56 186
MS.gene004050.t1 MTR_4g022370 38.857 350 154 12 1 317 1 323 2.14e-55 185
MS.gene004050.t1 MTR_7g024670 59.028 144 52 4 1 139 1 142 1.75e-46 162
MS.gene004050.t1 MTR_5g031440 87.500 56 7 0 70 125 38 93 1.18e-32 124
MS.gene004050.t1 MTR_3g077750 54.867 113 44 3 48 160 37 142 1.63e-32 124
MS.gene004050.t1 MTR_4g088580 66.667 81 23 1 76 156 56 132 6.06e-32 123
MS.gene004050.t1 MTR_2g093220 80.328 61 12 0 69 129 34 94 1.66e-31 120
MS.gene004050.t1 MTR_4g461080 66.667 78 25 1 50 127 5 81 1.78e-31 119
MS.gene004050.t1 MTR_8g479350 60.674 89 26 1 75 163 68 147 1.88e-31 121
MS.gene004050.t1 MTR_8g068210 69.620 79 20 2 74 152 31 105 1.06e-30 119
MS.gene004050.t1 MTR_8g027295 57.732 97 39 1 62 158 11 105 1.21e-30 117
MS.gene004050.t1 MTR_8g079060 54.717 106 32 3 54 159 17 106 8.83e-30 114
MS.gene004050.t1 MTR_3g091820 67.089 79 18 2 56 134 28 98 9.97e-30 115
MS.gene004050.t1 MTR_1g077600 60.000 80 30 1 52 129 18 97 2.90e-29 114
MS.gene004050.t1 MTR_1g077600 60.000 80 30 1 52 129 18 97 3.80e-29 114
MS.gene004050.t1 MTR_3g090430 63.514 74 27 0 56 129 32 105 9.58e-29 113
MS.gene004050.t1 MTR_8g015840 58.750 80 30 1 59 135 1 80 1.38e-28 110
MS.gene004050.t1 MTR_2g014060 67.606 71 22 1 57 126 6 76 2.20e-28 112
MS.gene004050.t1 MTR_4g109980 82.692 52 9 0 74 125 39 90 3.37e-28 112
MS.gene004050.t1 MTR_2g014170 88.000 50 6 0 74 123 44 93 4.94e-28 111
MS.gene004050.t1 MTR_1g056810 63.636 77 27 1 77 153 14 89 6.24e-28 110
MS.gene004050.t1 MTR_2g059540 84.000 50 8 0 74 123 30 79 8.89e-27 105
MS.gene004050.t1 MTR_4g089095 71.186 59 15 1 75 133 50 106 5.09e-25 102
MS.gene004050.t1 MTR_4g089095 71.186 59 15 1 75 133 16 72 6.28e-25 102
MS.gene004050.t1 MTR_2g013370 66.129 62 16 1 74 135 45 101 2.07e-24 100
MS.gene004050.t1 MTR_8g044220 66.102 59 20 0 71 129 114 172 2.83e-24 102
MS.gene004050.t1 MTR_2g016030 63.380 71 25 1 72 142 44 113 3.56e-24 97.1
MS.gene004050.t1 MTR_6g027450 63.333 60 22 0 68 127 82 141 7.32e-24 100
MS.gene004050.t1 MTR_6g012450 66.667 60 20 0 68 127 129 188 1.06e-23 101
MS.gene004050.t1 MTR_4g082060 58.571 70 26 1 58 127 108 174 1.82e-23 100
MS.gene004050.t1 MTR_7g010950 49.412 85 43 0 68 152 128 212 2.04e-23 100
MS.gene004050.t1 MTR_7g086780 60.000 70 25 2 60 129 86 152 2.13e-23 100
MS.gene004050.t1 MTR_3g435480 62.687 67 24 1 68 134 129 194 3.15e-23 100
MS.gene004050.t1 MTR_6g027460 61.667 60 23 0 68 127 89 148 4.58e-23 98.6
MS.gene004050.t1 MTR_5g041420 43.820 89 50 0 39 127 102 190 1.30e-21 94.0
MS.gene004050.t1 MTR_5g041530 69.091 55 16 1 77 131 142 195 1.52e-20 91.7
MS.gene004050.t1 MTR_5g041380 48.780 82 41 1 59 140 111 191 5.81e-20 90.1
MS.gene004050.t1 MTR_5g041400 50.725 69 34 0 59 127 103 171 1.25e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004050.t1 AT3G55370 39.093 353 136 14 1 324 1 303 9.35e-52 174
MS.gene004050.t1 AT3G55370 42.910 268 102 9 1 251 1 234 1.33e-51 174
MS.gene004050.t1 AT3G55370 39.809 314 118 11 32 324 85 348 8.04e-49 167
MS.gene004050.t1 AT1G07640 42.308 286 145 10 57 335 3 275 1.36e-48 164
MS.gene004050.t1 AT1G07640 42.105 285 147 9 57 335 59 331 6.20e-48 164
MS.gene004050.t1 AT1G07640 42.105 285 147 9 57 335 67 339 7.87e-48 164
MS.gene004050.t1 AT2G37590 53.846 156 32 2 1 129 1 143 8.30e-47 161
MS.gene004050.t1 AT2G28810 36.685 368 160 15 1 332 1 331 1.27e-45 159
MS.gene004050.t1 AT5G02460 88.158 76 9 0 54 129 74 149 4.71e-44 156
MS.gene004050.t1 AT2G28810 38.776 294 125 11 57 332 58 314 1.79e-42 150
MS.gene004050.t1 AT5G60200 76.389 72 13 1 70 141 50 117 1.73e-34 127
MS.gene004050.t1 AT1G51700 58.889 90 29 1 70 159 28 109 1.18e-31 118
MS.gene004050.t1 AT5G60850 62.791 86 25 2 74 159 50 128 2.35e-31 120
MS.gene004050.t1 AT3G45610 53.409 88 39 1 70 155 35 122 2.93e-31 118
MS.gene004050.t1 AT4G38000 77.049 61 14 0 69 129 35 95 2.20e-30 116
MS.gene004050.t1 AT5G65590 81.034 58 11 0 75 132 41 98 4.62e-30 117
MS.gene004050.t1 AT3G21270 53.000 100 28 2 75 155 29 128 3.61e-29 111
MS.gene004050.t1 AT4G24060 50.000 112 56 0 69 180 47 158 1.79e-28 113
MS.gene004050.t1 AT1G28310 79.661 59 11 1 75 133 27 84 3.46e-28 111
MS.gene004050.t1 AT3G52440 70.588 68 16 1 77 144 27 90 4.07e-28 110
MS.gene004050.t1 AT3G52440 70.588 68 16 1 77 144 46 109 4.71e-28 110
MS.gene004050.t1 AT2G28510 73.846 65 13 1 74 138 47 107 5.21e-28 110
MS.gene004050.t1 AT1G28310 79.661 59 11 1 75 133 51 108 5.67e-28 111
MS.gene004050.t1 AT5G62940 77.193 57 13 0 69 125 67 123 7.31e-28 112
MS.gene004050.t1 AT5G66940 65.714 70 21 1 75 144 32 98 2.27e-27 107
MS.gene004050.t1 AT3G61850 58.889 90 29 2 71 156 58 143 2.36e-27 108
MS.gene004050.t1 AT3G61850 58.889 90 29 2 71 156 58 143 2.36e-27 108
MS.gene004050.t1 AT3G61850 58.889 90 29 2 71 156 70 155 2.80e-27 108
MS.gene004050.t1 AT3G61850 58.889 90 29 2 71 156 58 143 3.07e-27 109
MS.gene004050.t1 AT1G21340 71.875 64 12 1 74 137 37 94 3.29e-27 108
MS.gene004050.t1 AT3G61850 58.889 90 29 2 71 156 70 155 3.78e-27 109
MS.gene004050.t1 AT4G21050 47.581 124 50 3 68 190 17 126 4.39e-27 106
MS.gene004050.t1 AT3G50410 70.690 58 17 0 75 132 30 87 1.44e-26 106
MS.gene004050.t1 AT2G46590 56.250 80 35 0 43 122 36 115 2.89e-26 107
MS.gene004050.t1 AT2G46590 56.250 80 35 0 43 122 48 127 3.49e-26 107
MS.gene004050.t1 AT4G00940 63.514 74 23 2 59 129 50 122 1.18e-25 104
MS.gene004050.t1 AT4G00940 63.514 74 23 2 59 129 50 122 1.18e-25 104
MS.gene004050.t1 AT4G00940 63.514 74 23 2 59 129 50 122 1.18e-25 104
MS.gene004050.t1 AT1G64620 66.176 68 23 0 56 123 30 97 4.00e-25 104
MS.gene004050.t1 AT1G47655 70.000 60 16 1 66 123 18 77 1.90e-24 99.4
MS.gene004050.t1 AT2G34140 58.442 77 28 2 53 129 39 111 3.70e-24 97.4
MS.gene004050.t1 AT1G29160 69.492 59 17 1 71 129 58 115 1.20e-23 96.3
MS.gene004050.t1 AT4G21040 56.250 80 30 1 77 156 27 101 3.24e-23 96.7
MS.gene004050.t1 AT5G39660 66.667 63 20 1 67 129 130 191 3.63e-23 100
MS.gene004050.t1 AT5G39660 66.667 63 20 1 67 129 130 191 3.63e-23 100
MS.gene004050.t1 AT5G39660 66.667 63 20 1 67 129 130 191 3.63e-23 100
MS.gene004050.t1 AT1G26790 54.412 68 31 0 60 127 96 163 4.12e-23 99.0
MS.gene004050.t1 AT5G62430 56.000 75 31 2 58 131 36 109 4.22e-23 97.8
MS.gene004050.t1 AT3G47500 65.000 60 21 0 68 127 103 162 8.22e-23 99.0
MS.gene004050.t1 AT1G69570 61.667 60 23 0 68 127 125 184 2.12e-22 97.4
MS.gene004050.t1 AT4G21080 63.492 63 18 1 77 139 27 84 7.52e-22 93.2
MS.gene004050.t1 AT4G21030 54.098 61 25 1 68 128 17 74 1.88e-15 74.3

Find 85 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTTGCTTCTTGATGATT+TGG 0.200308 4.2:+41494277 None:intergenic
GCAAGACATGTAGGCGTTAT+TGG 0.207030 4.2:-41494030 MS.gene004050:CDS
GTTGTTGTTGATGTTGTTCT+TGG 0.217968 4.2:+41493738 None:intergenic
TCAAGAGCCACCGGTTAAAA+TGG 0.246428 4.2:-41493465 MS.gene004050:CDS
TCTTGAGAGTTTGTTGAATT+AGG 0.247926 4.2:+41493920 None:intergenic
ACCAACAACAATACTATTCT+TGG 0.250684 4.2:-41493385 MS.gene004050:CDS
GTTTCTGCTTCTGCTGTTGC+TGG 0.257390 4.2:-41493653 MS.gene004050:CDS
GATCATATGGGGTTTCAAAT+TGG 0.271163 4.2:-41493707 MS.gene004050:CDS
TATTCTTGGAATGATTTATC+AGG 0.288988 4.2:-41493371 MS.gene004050:CDS
TACTGGTGGCGGTGGTACTA+TGG 0.292815 4.2:-41494203 MS.gene004050:CDS
TTTACCTGGTGGTGCCAATT+AGG 0.304320 4.2:+41494479 None:intergenic
CCTTCTCATCATGAACTAAT+TGG 0.327970 4.2:-41493869 MS.gene004050:CDS
GTTATGTAGATCCTCCTAAT+TGG 0.350822 4.2:-41494493 MS.gene004050:CDS
AACATTGAAGCTGCAGCATC+TGG 0.352153 4.2:-41493524 MS.gene004050:CDS
AATTCCTAACCTACCCTTCA+TGG 0.365060 4.2:-41493828 MS.gene004050:CDS
ACAACAACAACAAGATCATA+TGG 0.370266 4.2:-41493720 MS.gene004050:CDS
TCTGCTGTTGCTGGAGGTGT+TGG 0.398368 4.2:-41493644 MS.gene004050:CDS
TTTCAAATTGGTATTGGTAA+TGG 0.399190 4.2:-41493695 MS.gene004050:CDS
TCCAAGAATAGTATTGTTGT+TGG 0.406762 4.2:+41493384 None:intergenic
ATGGGGTTTCAAATTGGTAT+TGG 0.408013 4.2:-41493701 MS.gene004050:CDS
CTACGACACCCTCCACCAAC+TGG 0.417773 4.2:+41493980 None:intergenic
GGACACTTTAGCGCTGGTTC+TGG 0.424523 4.2:+41494124 None:intergenic
TGAGAAGGGATTGCACTTGA+TGG 0.431346 4.2:+41493884 None:intergenic
GCTCGATCAGCCATGGAGCC+TGG 0.444337 4.2:+41494169 None:intergenic
CCTCAAGTGGGAGGTAGTAC+TGG 0.448394 4.2:-41494220 MS.gene004050:CDS
GCTGCAGCATCTGGATTTGT+TGG 0.454209 4.2:-41493515 MS.gene004050:CDS
CCAAATCATCAAGAAGCAAA+TGG 0.458921 4.2:-41494277 MS.gene004050:CDS
GATTCAAAAGTATTCAAGAA+AGG 0.460724 4.2:+41493590 None:intergenic
CTTAGAAATGTTCCAGTTGG+TGG 0.472325 4.2:-41493992 MS.gene004050:CDS
CAACAACAACAAGATCATAT+GGG 0.473282 4.2:-41493719 MS.gene004050:CDS
AATTTATCAAGAACATCTCT+TGG 0.474556 4.2:-41493425 MS.gene004050:CDS
CTCGATCAGACCAGGCTCCA+TGG 0.475078 4.2:-41494179 MS.gene004050:CDS
GAGATATAGCTTCAAACTCT+AGG 0.480948 4.2:-41493493 MS.gene004050:CDS
CCTCCCACTTGAGGTGGTAA+AGG 0.483237 4.2:+41494229 None:intergenic
TTTAGCGCTGGTTCTGGTGG+TGG 0.483860 4.2:+41494130 None:intergenic
GGTAGGTTAGGAATTGTTTG+TGG 0.484498 4.2:+41493836 None:intergenic
GAGGCCATGAAGGGTAGGTT+AGG 0.490552 4.2:+41493824 None:intergenic
AGAAATGTTCCAGTTGGTGG+AGG 0.497986 4.2:-41493989 MS.gene004050:CDS
CCAATTAGTTCATGATGAGA+AGG 0.516486 4.2:+41493869 None:intergenic
CACTTTAGCGCTGGTTCTGG+TGG 0.522746 4.2:+41494127 None:intergenic
GAAGTAGCAAAATTTAGTGT+TGG 0.523027 4.2:+41494087 None:intergenic
TCCTCCTAATTGGCACCACC+AGG 0.528426 4.2:-41494483 MS.gene004050:intron
ACTGGTGGCGGTGGTACTAT+GGG 0.530847 4.2:-41494202 MS.gene004050:CDS
GGAAACGTTAACATGGGACT+TGG 0.531022 4.2:-41493773 MS.gene004050:CDS
GAGAAGGGATTGCACTTGAT+GGG 0.532889 4.2:+41493885 None:intergenic
CAACGAGGACACTTTAGCGC+TGG 0.540252 4.2:+41494118 None:intergenic
ACCTGGTGGTGCCAATTAGG+AGG 0.541280 4.2:+41494482 None:intergenic
TCTGCTTCTGCTGTTGCTGG+AGG 0.548891 4.2:-41493650 MS.gene004050:CDS
AGATATAGCTTCAAACTCTA+GGG 0.556521 4.2:-41493492 MS.gene004050:CDS
GGAGGTAGTACTGGTGGCGG+TGG 0.560481 4.2:-41494211 MS.gene004050:CDS
ATTCTGCATCGAGGCCATGA+AGG 0.561082 4.2:+41493814 None:intergenic
AATCTGAGTCGTTATGTCGT+TGG 0.570962 4.2:-41493794 MS.gene004050:CDS
CAATTAGTTCATGATGAGAA+GGG 0.573984 4.2:+41493870 None:intergenic
TGTCGTTGGAAACGTTAACA+TGG 0.575317 4.2:-41493780 MS.gene004050:CDS
TCCACCTTTACCACCTCAAG+TGG 0.577272 4.2:-41494233 MS.gene004050:CDS
ACTATGGGCTCGATCAGACC+AGG 0.580839 4.2:-41494187 MS.gene004050:CDS
TTCTGCATCGAGGCCATGAA+GGG 0.581643 4.2:+41493815 None:intergenic
CCCACTTGAGGTGGTAAAGG+TGG 0.581750 4.2:+41494232 None:intergenic
GAAATGTTCCAGTTGGTGGA+GGG 0.594932 4.2:-41493988 MS.gene004050:CDS
GCACTTAGAAATGTTCCAGT+TGG 0.600640 4.2:-41493995 MS.gene004050:CDS
AGAAGGGATTGCACTTGATG+GGG 0.604506 4.2:+41493886 None:intergenic
GTAGGCGTTATTGGACAAGA+GGG 0.605161 4.2:-41494021 MS.gene004050:CDS
TAGGGCTAATCAAGAGCCAC+CGG 0.605644 4.2:-41493474 MS.gene004050:CDS
GTCGTTGGAAACGTTAACAT+GGG 0.609381 4.2:-41493779 MS.gene004050:CDS
GTCACTTCTGCAAGACATGT+AGG 0.611130 4.2:-41494039 MS.gene004050:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 4.2:-41494022 MS.gene004050:CDS
GCATCGAGGCCATGAAGGGT+AGG 0.623817 4.2:+41493819 None:intergenic
AATTGCTGCAAATTACGCCA+TGG 0.629104 4.2:+41493614 None:intergenic
TAAACGAGCTCGATCAGCCA+TGG 0.633447 4.2:+41494162 None:intergenic
CATGTCTTGCAGAAGTGACG+AGG 0.635723 4.2:+41494043 None:intergenic
GTGGGAGGTAGTACTGGTGG+CGG 0.638124 4.2:-41494214 MS.gene004050:CDS
TTCTTGATAAATTTAAAGCG+CGG 0.644934 4.2:+41493435 None:intergenic
AACAACAACAAGATCATATG+GGG 0.646211 4.2:-41493718 MS.gene004050:CDS
CCACCTTTACCACCTCAAGT+GGG 0.662217 4.2:-41494232 MS.gene004050:CDS
CAAGTGGGAGGTAGTACTGG+TGG 0.664836 4.2:-41494217 MS.gene004050:CDS
GTACTACCTCCCACTTGAGG+TGG 0.666584 4.2:+41494223 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 4.2:-41494020 MS.gene004050:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 4.2:-41494019 MS.gene004050:CDS
CCAGTACTACCTCCCACTTG+AGG 0.681370 4.2:+41494220 None:intergenic
CCTTTACCACCTCAAGTGGG+AGG 0.700568 4.2:-41494229 MS.gene004050:CDS
GAGGTGTTGGAGTTGAACCA+TGG 0.701694 4.2:-41493631 MS.gene004050:CDS
TTGAATAGAAGAGAAAACCA+TGG 0.707467 4.2:+41494517 None:intergenic
AGAAGTGACGAGGTTGAGTG+AGG 0.724186 4.2:+41494053 None:intergenic
GTGTTGGTTGAATCGCAACG+AGG 0.733913 4.2:+41494103 None:intergenic
ACGACTCAGATTCTGCATCG+AGG 0.741695 4.2:+41493805 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAAAATTATTGTGA+TGG + chr4.2:41493521-41493540 None:intergenic 10.0%
!! ATGGAAAGAAATTAAAAAAA+GGG + chr4.2:41493545-41493564 None:intergenic 15.0%
!! ATGAAAATTAAAACTTTACC+TGG + chr4.2:41493399-41493418 None:intergenic 20.0%
!! GATGGAAAGAAATTAAAAAA+AGG + chr4.2:41493546-41493565 None:intergenic 20.0%
!!! AATAATTCATACACTTTTCA+TGG - chr4.2:41494307-41494326 MS.gene004050:intron 20.0%
!!! TTCACTTTCTTTTTTGAATT+TGG + chr4.2:41493428-41493447 None:intergenic 20.0%
! AAAATTAAAACTTTACCTGG+TGG + chr4.2:41493396-41493415 None:intergenic 25.0%
! AATTTATCAAGAACATCTCT+TGG - chr4.2:41494436-41494455 MS.gene004050:intron 25.0%
! ATATTCAAGAAGATGAAGTA+AGG + chr4.2:41493485-41493504 None:intergenic 25.0%
! TATTCTTGGAATGATTTATC+AGG - chr4.2:41494490-41494509 MS.gene004050:CDS 25.0%
!! TTCTTGATAAATTTAAAGCG+CGG + chr4.2:41494429-41494448 None:intergenic 25.0%
!! TTGAATTAGGAGATTTTGAA+CGG + chr4.2:41493931-41493950 None:intergenic 25.0%
!!! GATTCAAAAGTATTCAAGAA+AGG + chr4.2:41494274-41494293 None:intergenic 25.0%
!!! TTTCAAATTGGTATTGGTAA+TGG - chr4.2:41494166-41494185 MS.gene004050:CDS 25.0%
AACAACAACAAGATCATATG+GGG - chr4.2:41494143-41494162 MS.gene004050:CDS 30.0%
ACAACAACAACAAGATCATA+TGG - chr4.2:41494141-41494160 MS.gene004050:CDS 30.0%
ACCAACAACAATACTATTCT+TGG - chr4.2:41494476-41494495 MS.gene004050:intron 30.0%
AGATATAGCTTCAAACTCTA+GGG - chr4.2:41494369-41494388 MS.gene004050:intron 30.0%
CAACAACAACAAGATCATAT+GGG - chr4.2:41494142-41494161 MS.gene004050:CDS 30.0%
CAATTAGTTCATGATGAGAA+GGG + chr4.2:41493994-41494013 None:intergenic 30.0%
GAAGTAGCAAAATTTAGTGT+TGG + chr4.2:41493777-41493796 None:intergenic 30.0%
TCCAAGAATAGTATTGTTGT+TGG + chr4.2:41494480-41494499 None:intergenic 30.0%
! ATGGTTTTCTCTTCTATTCA+AGG - chr4.2:41493346-41493365 MS.gene004050:CDS 30.0%
!! TCTTGAGAGTTTGTTGAATT+AGG + chr4.2:41493944-41493963 None:intergenic 30.0%
ATGGGGTTTCAAATTGGTAT+TGG - chr4.2:41494160-41494179 MS.gene004050:CDS 35.0%
CCAAATCATCAAGAAGCAAA+TGG - chr4.2:41493584-41493603 MS.gene004050:CDS 35.0%
CCAATTAGTTCATGATGAGA+AGG + chr4.2:41493995-41494014 None:intergenic 35.0%
CCTTCTCATCATGAACTAAT+TGG - chr4.2:41493992-41494011 MS.gene004050:CDS 35.0%
GAGATATAGCTTCAAACTCT+AGG - chr4.2:41494368-41494387 MS.gene004050:intron 35.0%
GATCATATGGGGTTTCAAAT+TGG - chr4.2:41494154-41494173 MS.gene004050:CDS 35.0%
GTTATGTAGATCCTCCTAAT+TGG - chr4.2:41493368-41493387 MS.gene004050:CDS 35.0%
TTGCTGCAGGTTAAAATAGA+TGG + chr4.2:41493564-41493583 None:intergenic 35.0%
! ACTTTTGAATCAAACTCTGC+TGG - chr4.2:41494283-41494302 MS.gene004050:CDS 35.0%
! CCATTTGCTTCTTGATGATT+TGG + chr4.2:41493587-41493606 None:intergenic 35.0%
! GTTGTTGTTGATGTTGTTCT+TGG + chr4.2:41494126-41494145 None:intergenic 35.0%
!! CGGTTTTCTTCCATTTTAAC+CGG + chr4.2:41494409-41494428 None:intergenic 35.0%
!! TTTTCTTCCATTTTAACCGG+TGG + chr4.2:41494406-41494425 None:intergenic 35.0%
AATCTGAGTCGTTATGTCGT+TGG - chr4.2:41494067-41494086 MS.gene004050:CDS 40.0%
AATTCCTAACCTACCCTTCA+TGG - chr4.2:41494033-41494052 MS.gene004050:CDS 40.0%
AATTGCTGCAAATTACGCCA+TGG + chr4.2:41494250-41494269 None:intergenic 40.0%
CTTAGAAATGTTCCAGTTGG+TGG - chr4.2:41493869-41493888 MS.gene004050:CDS 40.0%
GCACTTAGAAATGTTCCAGT+TGG - chr4.2:41493866-41493885 MS.gene004050:CDS 40.0%
GGTAGGTTAGGAATTGTTTG+TGG + chr4.2:41494028-41494047 None:intergenic 40.0%
GTCGTTGGAAACGTTAACAT+GGG - chr4.2:41494082-41494101 MS.gene004050:CDS 40.0%
TGTCGTTGGAAACGTTAACA+TGG - chr4.2:41494081-41494100 MS.gene004050:CDS 40.0%
AACATTGAAGCTGCAGCATC+TGG - chr4.2:41494337-41494356 MS.gene004050:intron 45.0%
AGAAGGGATTGCACTTGATG+GGG + chr4.2:41493978-41493997 None:intergenic 45.0%
GAGAAGGGATTGCACTTGAT+GGG + chr4.2:41493979-41493998 None:intergenic 45.0%
GCAAGACATGTAGGCGTTAT+TGG - chr4.2:41493831-41493850 MS.gene004050:CDS 45.0%
GGAAACGTTAACATGGGACT+TGG - chr4.2:41494088-41494107 MS.gene004050:CDS 45.0%
GTAGGCGTTATTGGACAAGA+GGG - chr4.2:41493840-41493859 MS.gene004050:CDS 45.0%
GTCACTTCTGCAAGACATGT+AGG - chr4.2:41493822-41493841 MS.gene004050:CDS 45.0%
TAGGCGTTATTGGACAAGAG+GGG - chr4.2:41493841-41493860 MS.gene004050:CDS 45.0%
TGAGAAGGGATTGCACTTGA+TGG + chr4.2:41493980-41493999 None:intergenic 45.0%
TGTAGGCGTTATTGGACAAG+AGG - chr4.2:41493839-41493858 MS.gene004050:CDS 45.0%
! TCAAGAGCCACCGGTTAAAA+TGG - chr4.2:41494396-41494415 MS.gene004050:intron 45.0%
! TTTACCTGGTGGTGCCAATT+AGG + chr4.2:41493385-41493404 None:intergenic 45.0%
!! AGAAATGTTCCAGTTGGTGG+AGG - chr4.2:41493872-41493891 MS.gene004050:CDS 45.0%
!! CTTGATGATTTGGTTGCTGC+AGG + chr4.2:41493577-41493596 None:intergenic 45.0%
!! GAAATGTTCCAGTTGGTGGA+GGG - chr4.2:41493873-41493892 MS.gene004050:CDS 45.0%
ACGACTCAGATTCTGCATCG+AGG + chr4.2:41494059-41494078 None:intergenic 50.0%
AGAAGTGACGAGGTTGAGTG+AGG + chr4.2:41493811-41493830 None:intergenic 50.0%
AGGCGTTATTGGACAAGAGG+GGG - chr4.2:41493842-41493861 MS.gene004050:CDS 50.0%
ATTCTGCATCGAGGCCATGA+AGG + chr4.2:41494050-41494069 None:intergenic 50.0%
CATGTCTTGCAGAAGTGACG+AGG + chr4.2:41493821-41493840 None:intergenic 50.0%
CCACCTTTACCACCTCAAGT+GGG - chr4.2:41493629-41493648 MS.gene004050:CDS 50.0%
GCTGCAGCATCTGGATTTGT+TGG - chr4.2:41494346-41494365 MS.gene004050:intron 50.0%
GTTTCTGCTTCTGCTGTTGC+TGG - chr4.2:41494208-41494227 MS.gene004050:CDS 50.0%
TAAACGAGCTCGATCAGCCA+TGG + chr4.2:41493702-41493721 None:intergenic 50.0%
TAGGGCTAATCAAGAGCCAC+CGG - chr4.2:41494387-41494406 MS.gene004050:intron 50.0%
TCCACCTTTACCACCTCAAG+TGG - chr4.2:41493628-41493647 MS.gene004050:CDS 50.0%
TTCTGCATCGAGGCCATGAA+GGG + chr4.2:41494049-41494068 None:intergenic 50.0%
!! GAGGTGTTGGAGTTGAACCA+TGG - chr4.2:41494230-41494249 MS.gene004050:CDS 50.0%
!! GTGTTGGTTGAATCGCAACG+AGG + chr4.2:41493761-41493780 None:intergenic 50.0%
ACTATGGGCTCGATCAGACC+AGG - chr4.2:41493674-41493693 MS.gene004050:CDS 55.0%
CAACGAGGACACTTTAGCGC+TGG + chr4.2:41493746-41493765 None:intergenic 55.0%
CCAGTACTACCTCCCACTTG+AGG + chr4.2:41493644-41493663 None:intergenic 55.0%
CCCACTTGAGGTGGTAAAGG+TGG + chr4.2:41493632-41493651 None:intergenic 55.0%
CCTCAAGTGGGAGGTAGTAC+TGG - chr4.2:41493641-41493660 MS.gene004050:CDS 55.0%
CCTCCCACTTGAGGTGGTAA+AGG + chr4.2:41493635-41493654 None:intergenic 55.0%
CCTTTACCACCTCAAGTGGG+AGG - chr4.2:41493632-41493651 MS.gene004050:CDS 55.0%
GAGGCCATGAAGGGTAGGTT+AGG + chr4.2:41494040-41494059 None:intergenic 55.0%
GTACTACCTCCCACTTGAGG+TGG + chr4.2:41493641-41493660 None:intergenic 55.0%
TCCTCCTAATTGGCACCACC+AGG - chr4.2:41493378-41493397 MS.gene004050:CDS 55.0%
TCTGCTTCTGCTGTTGCTGG+AGG - chr4.2:41494211-41494230 MS.gene004050:CDS 55.0%
! ACCTGGTGGTGCCAATTAGG+AGG + chr4.2:41493382-41493401 None:intergenic 55.0%
! CAAGTGGGAGGTAGTACTGG+TGG - chr4.2:41493644-41493663 MS.gene004050:CDS 55.0%
! TCTGCTGTTGCTGGAGGTGT+TGG - chr4.2:41494217-41494236 MS.gene004050:CDS 55.0%
!! ACTGGTGGCGGTGGTACTAT+GGG - chr4.2:41493659-41493678 MS.gene004050:CDS 55.0%
!! CACTTTAGCGCTGGTTCTGG+TGG + chr4.2:41493737-41493756 None:intergenic 55.0%
!! GGACACTTTAGCGCTGGTTC+TGG + chr4.2:41493740-41493759 None:intergenic 55.0%
!! TACTGGTGGCGGTGGTACTA+TGG - chr4.2:41493658-41493677 MS.gene004050:CDS 55.0%
!! TTTAGCGCTGGTTCTGGTGG+TGG + chr4.2:41493734-41493753 None:intergenic 55.0%
CTACGACACCCTCCACCAAC+TGG + chr4.2:41493884-41493903 None:intergenic 60.0%
CTCGATCAGACCAGGCTCCA+TGG - chr4.2:41493682-41493701 MS.gene004050:CDS 60.0%
GCATCGAGGCCATGAAGGGT+AGG + chr4.2:41494045-41494064 None:intergenic 60.0%
!! GTGGGAGGTAGTACTGGTGG+CGG - chr4.2:41493647-41493666 MS.gene004050:CDS 60.0%
GCTCGATCAGCCATGGAGCC+TGG + chr4.2:41493695-41493714 None:intergenic 65.0%
!! GGAGGTAGTACTGGTGGCGG+TGG - chr4.2:41493650-41493669 MS.gene004050:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 41493346 41494537 41493346 ID=MS.gene004050
chr4.2 mRNA 41493346 41494537 41493346 ID=MS.gene004050.t1;Parent=MS.gene004050
chr4.2 exon 41494484 41494537 41494484 ID=MS.gene004050.t1.exon1;Parent=MS.gene004050.t1
chr4.2 CDS 41494484 41494537 41494484 ID=cds.MS.gene004050.t1;Parent=MS.gene004050.t1
chr4.2 exon 41493346 41494302 41493346 ID=MS.gene004050.t1.exon2;Parent=MS.gene004050.t1
chr4.2 CDS 41493346 41494302 41493346 ID=cds.MS.gene004050.t1;Parent=MS.gene004050.t1
Gene Sequence

>MS.gene004050

ATGGTTTTCTCTTCTATTCAAGGTTATGTAGATCCTCCTAATTGGCACCACCAGGTAAAGTTTTAATTTTCATCAAATTCCAAATTCAAAAAAGAAAGTGAAAAAAAAAACTTCAAAAAGATGAATCATTCATATTCCTTACTTCATCTTCTTGAATATACTAATTTTTTTTCCATCACAATAATTTTTTAAAATTCCCTTTTTTTAATTTCTTTCCATCTATTTTAACCTGCAGCAACCAAATCATCAAGAAGCAAATGGAAGCAATAACACTCAACTTCTTCCACCTTTACCACCTCAAGTGGGAGGTAGTACTGGTGGCGGTGGTACTATGGGCTCGATCAGACCAGGCTCCATGGCTGATCGAGCTCGTTTAGCAAAACTACCACCACCAGAACCAGCGCTAAAGTGTCCTCGTTGCGATTCAACCAACACTAAATTTTGCTACTTCAACAACTACAGCCTCACTCAACCTCGTCACTTCTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCACTTAGAAATGTTCCAGTTGGTGGAGGGTGTCGTAGAAACAACAAGAAAAACAAAAGAAGCCGTTCAAAATCTCCTAATTCAACAAACTCTCAAGAAAAACAAACTTCCCCATCAAGTGCAATCCCTTCTCATCATGAACTAATTGGTCATCAGTTACCACAAACAATTCCTAACCTACCCTTCATGGCCTCGATGCAGAATCTGAGTCGTTATGTCGTTGGAAACGTTAACATGGGACTTGGTTTGCGTGAGATCCAAGAACAACATCAACAACAACAACAACAAGATCATATGGGGTTTCAAATTGGTATTGGTAATGGTAACTCAACTTCTGTTTCTGTTTCTGCTTCTGCTGTTGCTGGAGGTGTTGGAGTTGAACCATGGCGTAATTTGCAGCAATTTCCTTTCTTGAATACTTTTGAATCAAACTCTGCTGGAAATAATTCATACACTTTTCATGGAGAAAGTAACATTGAAGCTGCAGCATCTGGATTTGTTGGAGATATAGCTTCAAACTCTAGGGCTAATCAAGAGCCACCGGTTAAAATGGAAGAAAACCGCGCTTTAAATTTATCAAGAACATCTCTTGGTGTCTCTGAACATAATAACCAACAACAATACTATTCTTGGAATGATTTATCAGGTTCTTCAACTACTCATCTCTTATAA
Protein sequence

>MS.gene004050.t1

MVFSSIQGYVDPPNWHHQQPNHQEANGSNNTQLLPPLPPQVGGSTGGGGTMGSIRPGSMADRARLAKLPPPEPALKCPRCDSTNTKFCYFNNYSLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKKNKRSRSKSPNSTNSQEKQTSPSSAIPSHHELIGHQLPQTIPNLPFMASMQNLSRYVVGNVNMGLGLREIQEQHQQQQQQDHMGFQIGIGNGNSTSVSVSASAVAGGVGVEPWRNLQQFPFLNTFESNSAGNNSYTFHGESNIEAAASGFVGDIASNSRANQEPPVKMEENRALNLSRTSLGVSEHNNQQQYYSWNDLSGSSTTHLL