AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30954

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30954.t1 MTR_2g096740 95.752 306 7 5 1 303 1 303 0.0 586
MS.gene30954.t1 MTR_2g096740 90.850 306 7 6 1 303 1 288 0.0 548
MS.gene30954.t1 MTR_4g063780 42.500 360 115 17 1 297 1 331 3.18e-63 203
MS.gene30954.t1 MTR_4g022370 40.549 328 108 17 1 261 1 308 1.79e-47 163
MS.gene30954.t1 MTR_7g059400 37.818 275 115 6 1 238 1 256 3.45e-43 151
MS.gene30954.t1 MTR_7g024670 52.500 160 45 5 1 146 1 143 9.67e-42 148
MS.gene30954.t1 MTR_8g068210 56.000 100 41 1 55 154 12 108 5.36e-33 124
MS.gene30954.t1 MTR_8g479350 62.069 87 29 1 78 164 68 150 2.24e-32 122
MS.gene30954.t1 MTR_3g077750 52.475 101 48 0 28 128 11 111 3.00e-31 119
MS.gene30954.t1 MTR_4g088580 68.919 74 18 1 79 147 56 129 3.74e-31 120
MS.gene30954.t1 MTR_8g015840 76.119 67 14 1 73 139 15 79 8.41e-31 115
MS.gene30954.t1 MTR_4g461080 76.923 65 14 1 67 130 17 81 1.26e-30 116
MS.gene30954.t1 MTR_2g093220 64.935 77 27 0 56 132 18 94 1.35e-30 117
MS.gene30954.t1 MTR_5g031440 82.143 56 10 0 73 128 38 93 2.27e-30 117
MS.gene30954.t1 MTR_3g091820 71.642 67 19 0 67 133 32 98 2.33e-30 116
MS.gene30954.t1 MTR_3g090430 70.149 67 20 0 68 134 41 107 7.42e-30 115
MS.gene30954.t1 MTR_8g079060 52.000 100 44 2 71 166 22 121 1.21e-29 112
MS.gene30954.t1 MTR_4g109980 86.538 52 7 0 77 128 39 90 1.45e-29 114
MS.gene30954.t1 MTR_2g059540 90.000 50 5 0 77 126 30 79 2.04e-28 108
MS.gene30954.t1 MTR_8g027295 72.581 62 17 0 75 136 21 82 3.18e-28 110
MS.gene30954.t1 MTR_2g014170 75.362 69 17 0 77 145 44 112 4.24e-28 110
MS.gene30954.t1 MTR_2g014060 84.314 51 8 0 79 129 26 76 2.05e-27 109
MS.gene30954.t1 MTR_1g077600 68.333 60 19 0 75 134 40 99 6.95e-27 106
MS.gene30954.t1 MTR_1g077600 68.333 60 19 0 75 134 40 99 8.10e-27 107
MS.gene30954.t1 MTR_4g089095 75.862 58 13 1 78 135 50 106 8.41e-27 107
MS.gene30954.t1 MTR_4g089095 75.862 58 13 1 78 135 16 72 9.81e-27 105
MS.gene30954.t1 MTR_2g013370 50.538 93 27 2 42 134 28 101 1.12e-26 106
MS.gene30954.t1 MTR_1g056810 77.358 53 12 0 80 132 14 66 1.28e-26 106
MS.gene30954.t1 MTR_5g041420 44.118 102 57 0 30 131 90 191 6.96e-26 105
MS.gene30954.t1 MTR_6g012450 52.273 88 41 1 44 131 103 189 7.86e-26 107
MS.gene30954.t1 MTR_2g016030 56.410 78 30 1 64 141 37 110 9.91e-26 100
MS.gene30954.t1 MTR_8g044220 51.163 86 42 0 56 141 96 181 2.17e-25 105
MS.gene30954.t1 MTR_6g027450 58.824 68 28 0 65 132 76 143 4.53e-24 100
MS.gene30954.t1 MTR_7g010950 61.538 65 25 0 67 131 124 188 6.89e-24 101
MS.gene30954.t1 MTR_3g435480 57.534 73 30 1 59 131 118 189 1.19e-23 100
MS.gene30954.t1 MTR_7g086780 59.701 67 27 0 65 131 86 152 1.27e-23 100
MS.gene30954.t1 MTR_5g041530 47.778 90 47 0 42 131 104 193 1.53e-23 99.4
MS.gene30954.t1 MTR_6g027460 56.716 67 29 0 65 131 83 149 2.61e-23 98.6
MS.gene30954.t1 MTR_4g082060 64.912 57 20 0 75 131 119 175 7.24e-23 98.6
MS.gene30954.t1 MTR_5g041380 47.126 87 45 1 45 131 95 180 1.78e-21 93.6
MS.gene30954.t1 MTR_5g041400 53.333 75 32 1 57 131 101 172 7.26e-21 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30954.t1 AT3G55370 39.275 331 142 15 1 291 1 312 1.23e-48 165
MS.gene30954.t1 AT3G55370 42.126 254 99 10 1 224 1 236 2.86e-46 159
MS.gene30954.t1 AT3G55370 37.578 322 143 14 9 291 55 357 4.46e-42 149
MS.gene30954.t1 AT2G37590 51.351 148 55 4 1 134 1 145 1.49e-40 144
MS.gene30954.t1 AT2G28810 48.408 157 41 4 1 129 1 145 9.58e-38 137
MS.gene30954.t1 AT5G65590 72.500 80 17 1 65 139 23 102 8.57e-35 128
MS.gene30954.t1 AT5G02460 56.303 119 35 2 39 157 73 174 1.33e-34 130
MS.gene30954.t1 AT2G28810 61.702 94 24 1 36 129 47 128 7.13e-34 126
MS.gene30954.t1 AT1G07640 73.611 72 18 1 64 135 8 78 2.26e-33 124
MS.gene30954.t1 AT5G60200 65.333 75 26 0 71 145 48 122 4.58e-33 122
MS.gene30954.t1 AT1G07640 73.611 72 18 1 64 135 64 134 4.76e-33 124
MS.gene30954.t1 AT1G07640 73.611 72 18 1 64 135 72 142 7.73e-33 124
MS.gene30954.t1 AT1G51700 47.934 121 63 0 53 173 8 128 1.49e-32 119
MS.gene30954.t1 AT5G60850 67.532 77 23 1 77 151 50 126 2.62e-32 122
MS.gene30954.t1 AT5G66940 56.322 87 38 0 64 150 18 104 1.06e-30 115
MS.gene30954.t1 AT3G45610 63.889 72 26 0 68 139 30 101 2.77e-30 115
MS.gene30954.t1 AT5G62940 78.333 60 13 0 69 128 64 123 1.05e-29 116
MS.gene30954.t1 AT4G21050 57.647 85 33 1 64 148 13 94 1.48e-29 112
MS.gene30954.t1 AT2G28510 78.333 60 13 0 77 136 47 106 1.57e-29 114
MS.gene30954.t1 AT3G21270 86.792 53 7 0 78 130 29 81 1.81e-29 111
MS.gene30954.t1 AT3G52440 63.158 76 28 0 74 149 21 96 2.64e-29 112
MS.gene30954.t1 AT3G52440 63.158 76 28 0 74 149 40 115 3.98e-29 112
MS.gene30954.t1 AT1G28310 77.586 58 13 0 78 135 27 84 4.92e-29 113
MS.gene30954.t1 AT4G38000 73.770 61 16 0 72 132 35 95 7.11e-29 111
MS.gene30954.t1 AT1G28310 77.586 58 13 0 78 135 51 108 7.63e-29 113
MS.gene30954.t1 AT1G21340 71.667 60 17 0 76 135 36 95 1.85e-28 110
MS.gene30954.t1 AT3G50410 74.576 59 15 0 78 136 30 88 8.58e-28 108
MS.gene30954.t1 AT4G24060 66.667 66 22 0 67 132 42 107 1.32e-27 110
MS.gene30954.t1 AT3G61850 41.667 144 76 4 22 165 8 143 5.45e-27 107
MS.gene30954.t1 AT3G61850 41.667 144 76 4 22 165 8 143 5.45e-27 107
MS.gene30954.t1 AT3G61850 41.667 144 76 4 22 165 20 155 5.69e-27 107
MS.gene30954.t1 AT3G61850 41.958 143 75 4 23 165 9 143 8.15e-27 107
MS.gene30954.t1 AT3G61850 41.958 143 75 4 23 165 21 155 9.30e-27 107
MS.gene30954.t1 AT1G47655 68.182 66 20 1 62 126 12 77 2.44e-26 103
MS.gene30954.t1 AT4G00940 77.358 53 12 0 80 132 70 122 2.62e-25 102
MS.gene30954.t1 AT4G00940 77.358 53 12 0 80 132 70 122 2.62e-25 102
MS.gene30954.t1 AT4G00940 77.358 53 12 0 80 132 70 122 2.62e-25 102
MS.gene30954.t1 AT1G64620 68.852 61 19 0 67 127 38 98 3.45e-25 103
MS.gene30954.t1 AT2G34140 70.175 57 17 0 75 131 55 111 7.38e-25 98.6
MS.gene30954.t1 AT1G29160 70.175 57 17 0 75 131 59 115 1.15e-24 98.2
MS.gene30954.t1 AT2G46590 78.431 51 11 0 75 125 65 115 2.11e-24 101
MS.gene30954.t1 AT2G46590 78.431 51 11 0 75 125 77 127 2.26e-24 101
MS.gene30954.t1 AT5G39660 58.108 74 30 1 58 131 119 191 5.56e-24 101
MS.gene30954.t1 AT5G39660 58.108 74 30 1 58 131 119 191 5.56e-24 101
MS.gene30954.t1 AT5G39660 58.108 74 30 1 58 131 119 191 5.56e-24 101
MS.gene30954.t1 AT3G47500 48.352 91 40 2 47 131 74 163 6.60e-24 101
MS.gene30954.t1 AT1G69570 63.492 63 23 0 75 137 129 191 2.15e-23 99.4
MS.gene30954.t1 AT1G26790 56.164 73 29 1 59 131 95 164 2.56e-23 99.0
MS.gene30954.t1 AT5G62430 47.126 87 43 1 45 131 24 107 3.27e-23 97.4
MS.gene30954.t1 AT4G21040 59.701 67 23 1 80 146 27 89 1.75e-22 94.0
MS.gene30954.t1 AT4G21080 61.905 63 20 1 80 142 27 85 1.10e-21 92.0
MS.gene30954.t1 AT4G21030 49.296 71 32 1 70 140 17 83 1.93e-15 73.9

Find 73 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGACATGTAGGCGTTAT+TGG 0.219753 2.2:-5336801 MS.gene30954:CDS
TTGAACCAACAACTACTTCT+AGG 0.230645 2.2:-5336390 MS.gene30954:CDS
TTCTCATTACTATTTGATAT+TGG 0.260955 2.2:+5336682 None:intergenic
CAGTTTCCTTTCATGAATAA+TGG 0.306796 2.2:-5336463 MS.gene30954:CDS
TCAACAATTTCACTTTGAAA+TGG 0.326972 2.2:+5336409 None:intergenic
TGAAGAGATGTCATGTAATT+AGG 0.330958 2.2:+5336589 None:intergenic
TTATTCTCTGACATATTCAT+TGG 0.339957 2.2:+5336298 None:intergenic
CAACAATTTCACTTTGAAAT+GGG 0.353913 2.2:+5336410 None:intergenic
GTTGGAAACATGTTTCAAAT+TGG 0.387187 2.2:-5336547 MS.gene30954:CDS
GGAAGCAACTGATGTTGATT+TGG 0.388221 2.2:+5337060 None:intergenic
AGCTTGATCCGGTATCGAAC+CGG 0.392442 2.2:+5336990 None:intergenic
TCATGGTCTTGCATCTTCTT+CGG 0.406074 2.2:-5336242 MS.gene30954:CDS
CAAGAGGAGTTGAAGTTGAA+TGG 0.407718 2.2:+5336638 None:intergenic
ATGGTGCAGGCTCAATTATC+AGG 0.408827 2.2:-5337014 MS.gene30954:CDS
GATGCCTGCATCGGTTAAAT+CGG 0.412501 2.2:-5336356 MS.gene30954:CDS
TGTAATTAGGTCTATTAGTT+GGG 0.420123 2.2:+5336602 None:intergenic
ATGTAATTAGGTCTATTAGT+TGG 0.434589 2.2:+5336601 None:intergenic
CTTATTTAGATCCTCTCAAT+TGG 0.441172 2.2:-5337288 MS.gene30954:CDS
CATTCAACTTCAACTCCTCT+TGG 0.446006 2.2:-5336637 MS.gene30954:CDS
TGAAGTTCAAACTGGTCATC+AGG 0.450714 2.2:-5336950 MS.gene30954:CDS
TATCAGGCCCGGTTCGATAC+CGG 0.455922 2.2:-5336998 MS.gene30954:CDS
CTACGACACCCTCCACCAAC+AGG 0.460856 2.2:+5336751 None:intergenic
AAAAGTGTCTTGGTTGAGAC+AGG 0.461945 2.2:+5336824 None:intergenic
TTGAGAGGATCTAAATAAGA+TGG 0.471759 2.2:+5337292 None:intergenic
ACATGTTTCCAACACCGAAC+CGG 0.484940 2.2:+5336557 None:intergenic
GGTGATTATAGTTCAAGTAC+TGG 0.489772 2.2:-5336526 MS.gene30954:CDS
TGAACCAACAACTACTTCTA+GGG 0.497338 2.2:-5336389 MS.gene30954:CDS
GCCTGAGCTTGAGCTTGATC+CGG 0.498212 2.2:+5336979 None:intergenic
TGTTCCGATTTAACCGATGC+AGG 0.498710 2.2:+5336352 None:intergenic
GCAGCTCATGAAGTTCAAAC+TGG 0.502505 2.2:-5336958 MS.gene30954:CDS
CTTCAGAATCTGAACCGGTT+CGG 0.507338 2.2:-5336571 MS.gene30954:CDS
CATCTCTTCAGAATCTGAAC+CGG 0.507412 2.2:-5336576 MS.gene30954:CDS
AATCTGAACCGGTTCGGTGT+TGG 0.507539 2.2:-5336565 MS.gene30954:CDS
TACTACTTTGATGCCTGCAT+CGG 0.514344 2.2:-5336365 MS.gene30954:CDS
GTAACCCTAGAAGTAGTTGT+TGG 0.518165 2.2:+5336385 None:intergenic
TTAAGAAATGTTCCTGTTGG+TGG 0.525025 2.2:-5336763 MS.gene30954:CDS
ACCATTACAATAATTCATCA+TGG 0.529637 2.2:-5336273 MS.gene30954:CDS
ACCGGATCAAGCTCAAGCTC+AGG 0.531426 2.2:-5336980 MS.gene30954:CDS
CATGTCTTGCAAAAGTGTCT+TGG 0.535988 2.2:+5336814 None:intergenic
TCATCACAGCCACATGGTGC+AGG 0.545230 2.2:-5337027 MS.gene30954:CDS
TCAAAACCATTATTCATGAA+AGG 0.546534 2.2:+5336457 None:intergenic
TCCATGATGAATTATTGTAA+TGG 0.550629 2.2:+5336272 None:intergenic
CACTTCAAAGCTGTCTCTGA+AGG 0.551798 2.2:+5336898 None:intergenic
TGGAGGAGACGGAGGAGTTG+TGG 0.552898 2.2:-5336503 MS.gene30954:CDS
AGAAATGTTCCTGTTGGTGG+AGG 0.558097 2.2:-5336760 MS.gene30954:CDS
TCAAGTACTGGTGGAGGAGA+CGG 0.565030 2.2:-5336514 MS.gene30954:CDS
ACGGAGGAGTTGTGGATCAG+TGG 0.567099 2.2:-5336495 MS.gene30954:CDS
CTTACTTGCTGCCAATTGAG+AGG 0.568314 2.2:+5337277 None:intergenic
AAATCGGAACACAACGGAGG+AGG 0.573191 2.2:-5336340 MS.gene30954:CDS
GCATTAAGAAATGTTCCTGT+TGG 0.573758 2.2:-5336766 MS.gene30954:CDS
GCTTGATCCGGTATCGAACC+GGG 0.575393 2.2:+5336991 None:intergenic
GCAGGCTCAATTATCAGGCC+CGG 0.575596 2.2:-5337009 MS.gene30954:CDS
AAGGTGGTGGTAACTTAGCT+TGG 0.577015 2.2:+5336917 None:intergenic
AAAGCTGTCTCTGAAGGTGG+TGG 0.586444 2.2:+5336904 None:intergenic
TATAGTTCAAGTACTGGTGG+AGG 0.593548 2.2:-5336520 MS.gene30954:CDS
GATTATAGTTCAAGTACTGG+TGG 0.600312 2.2:-5336523 MS.gene30954:CDS
GTAGGCGTTATTGGACAAGA+GGG 0.605161 2.2:-5336792 MS.gene30954:CDS
TGTGGCTGTGATGAGGATAG+TGG 0.611946 2.2:+5337036 None:intergenic
TGTAGGCGTTATTGGACAAG+AGG 0.616737 2.2:-5336793 MS.gene30954:CDS
TTCAAAGCTGTCTCTGAAGG+TGG 0.619079 2.2:+5336901 None:intergenic
GAAATGTTCCTGTTGGTGGA+GGG 0.644271 2.2:-5336759 MS.gene30954:CDS
CTATCCTCATCACAGCCACA+TGG 0.646501 2.2:-5337033 MS.gene30954:CDS
GGCTGTGATGAGGATAGTGG+TGG 0.646948 2.2:+5337039 None:intergenic
TGCACCATGTGGCTGTGATG+AGG 0.663808 2.2:+5337029 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 2.2:-5336791 MS.gene30954:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 2.2:-5336790 MS.gene30954:CDS
ATAATTGAGCCTGCACCATG+TGG 0.676504 2.2:+5337018 None:intergenic
AGTACTGGTGGAGGAGACGG+AGG 0.688303 2.2:-5336511 MS.gene30954:CDS
TGGAATAGAAGAGAAAACCA+TGG 0.712223 2.2:+5337312 None:intergenic
GGGTGTAAGAATTCACCAAG+AGG 0.726422 2.2:+5336622 None:intergenic
GTGTTGGTTGATTCACAACG+AGG 0.729681 2.2:+5336874 None:intergenic
GTTAAATCGGAACACAACGG+AGG 0.733463 2.2:-5336343 MS.gene30954:CDS
TCGGTTAAATCGGAACACAA+CGG 0.745806 2.2:-5336346 MS.gene30954:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGATATTATTATTACAA+AGG + chr2.2:5336336-5336355 None:intergenic 15.0%
!! TATTATTATTACAAAGGAAT+AGG + chr2.2:5336330-5336349 None:intergenic 15.0%
!! TCATATATAGATATATCACA+AGG + chr2.2:5336427-5336446 None:intergenic 20.0%
!! TTCTCATTACTATTTGATAT+TGG + chr2.2:5336856-5336875 None:intergenic 20.0%
!!! TAAATAAATTCACTCTTTTG+TGG + chr2.2:5336305-5336324 None:intergenic 20.0%
! ACCATTACAATAATTCATCA+TGG - chr2.2:5337262-5337281 MS.gene30954:intron 25.0%
! ATGTAATTAGGTCTATTAGT+TGG + chr2.2:5336937-5336956 None:intergenic 25.0%
! TCAAAACCATTATTCATGAA+AGG + chr2.2:5337081-5337100 None:intergenic 25.0%
! TCCATGATGAATTATTGTAA+TGG + chr2.2:5337266-5337285 None:intergenic 25.0%
! TGTAATGAAAAAAAATCTGC+AGG + chr2.2:5336367-5336386 None:intergenic 25.0%
! TGTAATTAGGTCTATTAGTT+GGG + chr2.2:5336936-5336955 None:intergenic 25.0%
! TTATTCTCTGACATATTCAT+TGG + chr2.2:5337240-5337259 None:intergenic 25.0%
!! ATTTGATATTGGAGATTTTG+AGG + chr2.2:5336845-5336864 None:intergenic 25.0%
!! GTAGTAACAAAATTTTGTGT+TGG + chr2.2:5336680-5336699 None:intergenic 25.0%
!! TCAACAATTTCACTTTGAAA+TGG + chr2.2:5337129-5337148 None:intergenic 25.0%
!! TTTGATATTGGAGATTTTGA+GGG + chr2.2:5336844-5336863 None:intergenic 25.0%
!!! CAACAATTTCACTTTGAAAT+GGG + chr2.2:5337128-5337147 None:intergenic 25.0%
ATATCACAAGGTTACAAGAA+AGG + chr2.2:5336415-5336434 None:intergenic 30.0%
CAGTTTCCTTTCATGAATAA+TGG - chr2.2:5337072-5337091 MS.gene30954:intron 30.0%
CTTATTTAGATCCTCTCAAT+TGG - chr2.2:5336247-5336266 MS.gene30954:CDS 30.0%
GTTGGAAACATGTTTCAAAT+TGG - chr2.2:5336988-5337007 MS.gene30954:CDS 30.0%
TATCACAAGGTTACAAGAAA+GGG + chr2.2:5336414-5336433 None:intergenic 30.0%
TGAAGAGATGTCATGTAATT+AGG + chr2.2:5336949-5336968 None:intergenic 30.0%
TTGAGAGGATCTAAATAAGA+TGG + chr2.2:5336246-5336265 None:intergenic 30.0%
GCATTAAGAAATGTTCCTGT+TGG - chr2.2:5336769-5336788 MS.gene30954:CDS 35.0%
GGTGATTATAGTTCAAGTAC+TGG - chr2.2:5337009-5337028 MS.gene30954:CDS 35.0%
TGAACCAACAACTACTTCTA+GGG - chr2.2:5337146-5337165 MS.gene30954:intron 35.0%
TTAAGAAATGTTCCTGTTGG+TGG - chr2.2:5336772-5336791 MS.gene30954:CDS 35.0%
TTGAACCAACAACTACTTCT+AGG - chr2.2:5337145-5337164 MS.gene30954:intron 35.0%
!! GATTATAGTTCAAGTACTGG+TGG - chr2.2:5337012-5337031 MS.gene30954:CDS 35.0%
!! TCATCATGGACTGATTTTCA+TGG - chr2.2:5337276-5337295 MS.gene30954:intron 35.0%
!!! AGTAGAGGTGTTACTCTTTT+TGG + chr2.2:5336821-5336840 None:intergenic 35.0%
CAAGAGGAGTTGAAGTTGAA+TGG + chr2.2:5336900-5336919 None:intergenic 40.0%
CATCTCTTCAGAATCTGAAC+CGG - chr2.2:5336959-5336978 MS.gene30954:CDS 40.0%
CATTCAACTTCAACTCCTCT+TGG - chr2.2:5336898-5336917 MS.gene30954:CDS 40.0%
GTAACCCTAGAAGTAGTTGT+TGG + chr2.2:5337153-5337172 None:intergenic 40.0%
TACTACTTTGATGCCTGCAT+CGG - chr2.2:5337170-5337189 MS.gene30954:intron 40.0%
TCGGTTAAATCGGAACACAA+CGG - chr2.2:5337189-5337208 MS.gene30954:intron 40.0%
TGAAGTTCAAACTGGTCATC+AGG - chr2.2:5336585-5336604 MS.gene30954:CDS 40.0%
! GACACTTTTGCAAGACATGT+AGG - chr2.2:5336725-5336744 MS.gene30954:CDS 40.0%
! GGAAGCAACTGATGTTGATT+TGG + chr2.2:5336478-5336497 None:intergenic 40.0%
! TCATGGTCTTGCATCTTCTT+CGG - chr2.2:5337293-5337312 MS.gene30954:CDS 40.0%
!! AAAAGTGTCTTGGTTGAGAC+AGG + chr2.2:5336714-5336733 None:intergenic 40.0%
!! CATGTCTTGCAAAAGTGTCT+TGG + chr2.2:5336724-5336743 None:intergenic 40.0%
!! TATAGTTCAAGTACTGGTGG+AGG - chr2.2:5337015-5337034 MS.gene30954:CDS 40.0%
ACATGTTTCCAACACCGAAC+CGG + chr2.2:5336981-5337000 None:intergenic 45.0%
ATAATTGAGCCTGCACCATG+TGG + chr2.2:5336520-5336539 None:intergenic 45.0%
ATGGTGCAGGCTCAATTATC+AGG - chr2.2:5336521-5336540 MS.gene30954:CDS 45.0%
CACTTCAAAGCTGTCTCTGA+AGG + chr2.2:5336640-5336659 None:intergenic 45.0%
CTTCAGAATCTGAACCGGTT+CGG - chr2.2:5336964-5336983 MS.gene30954:CDS 45.0%
GCAAGACATGTAGGCGTTAT+TGG - chr2.2:5336734-5336753 MS.gene30954:CDS 45.0%
GCAGCTCATGAAGTTCAAAC+TGG - chr2.2:5336577-5336596 MS.gene30954:CDS 45.0%
GGGTGTAAGAATTCACCAAG+AGG + chr2.2:5336916-5336935 None:intergenic 45.0%
GTAGGCGTTATTGGACAAGA+GGG - chr2.2:5336743-5336762 MS.gene30954:CDS 45.0%
GTTAAATCGGAACACAACGG+AGG - chr2.2:5337192-5337211 MS.gene30954:intron 45.0%
TAGGCGTTATTGGACAAGAG+GGG - chr2.2:5336744-5336763 MS.gene30954:CDS 45.0%
TGTAGGCGTTATTGGACAAG+AGG - chr2.2:5336742-5336761 MS.gene30954:CDS 45.0%
TGTTCCGATTTAACCGATGC+AGG + chr2.2:5337186-5337205 None:intergenic 45.0%
! AAGGTGGTGGTAACTTAGCT+TGG + chr2.2:5336621-5336640 None:intergenic 45.0%
! AGAAATGTTCCTGTTGGTGG+AGG - chr2.2:5336775-5336794 MS.gene30954:CDS 45.0%
! CTTACTTGCTGCCAATTGAG+AGG + chr2.2:5336261-5336280 None:intergenic 45.0%
! GAAATGTTCCTGTTGGTGGA+GGG - chr2.2:5336776-5336795 MS.gene30954:CDS 45.0%
! TTCAAAGCTGTCTCTGAAGG+TGG + chr2.2:5336637-5336656 None:intergenic 45.0%
!! GATGCCTGCATCGGTTAAAT+CGG - chr2.2:5337179-5337198 MS.gene30954:intron 45.0%
!! GTGTTGGTTGATTCACAACG+AGG + chr2.2:5336664-5336683 None:intergenic 45.0%
!! TGGAGATTTTGAGGGAGTAG+AGG + chr2.2:5336836-5336855 None:intergenic 45.0%
AAATCGGAACACAACGGAGG+AGG - chr2.2:5337195-5337214 MS.gene30954:intron 50.0%
AGGCGTTATTGGACAAGAGG+GGG - chr2.2:5336745-5336764 MS.gene30954:CDS 50.0%
CTATCCTCATCACAGCCACA+TGG - chr2.2:5336502-5336521 MS.gene30954:CDS 50.0%
TGTGGCTGTGATGAGGATAG+TGG + chr2.2:5336502-5336521 None:intergenic 50.0%
! AAAGCTGTCTCTGAAGGTGG+TGG + chr2.2:5336634-5336653 None:intergenic 50.0%
! AGCTTGATCCGGTATCGAAC+CGG + chr2.2:5336548-5336567 None:intergenic 50.0%
!! AATCTGAACCGGTTCGGTGT+TGG - chr2.2:5336970-5336989 MS.gene30954:CDS 50.0%
!! TCAAGTACTGGTGGAGGAGA+CGG - chr2.2:5337021-5337040 MS.gene30954:CDS 50.0%
ACCGGATCAAGCTCAAGCTC+AGG - chr2.2:5336555-5336574 MS.gene30954:CDS 55.0%
ACGGAGGAGTTGTGGATCAG+TGG - chr2.2:5337040-5337059 MS.gene30954:CDS 55.0%
GCAGGCTCAATTATCAGGCC+CGG - chr2.2:5336526-5336545 MS.gene30954:CDS 55.0%
GCCTGAGCTTGAGCTTGATC+CGG + chr2.2:5336559-5336578 None:intergenic 55.0%
TCATCACAGCCACATGGTGC+AGG - chr2.2:5336508-5336527 MS.gene30954:CDS 55.0%
TGCACCATGTGGCTGTGATG+AGG + chr2.2:5336509-5336528 None:intergenic 55.0%
! GCTTGATCCGGTATCGAACC+GGG + chr2.2:5336547-5336566 None:intergenic 55.0%
! GGCTGTGATGAGGATAGTGG+TGG + chr2.2:5336499-5336518 None:intergenic 55.0%
! TATCAGGCCCGGTTCGATAC+CGG - chr2.2:5336537-5336556 MS.gene30954:CDS 55.0%
CTACGACACCCTCCACCAAC+AGG + chr2.2:5336787-5336806 None:intergenic 60.0%
TGGAGGAGACGGAGGAGTTG+TGG - chr2.2:5337032-5337051 MS.gene30954:CDS 60.0%
!! AGTACTGGTGGAGGAGACGG+AGG - chr2.2:5337024-5337043 MS.gene30954:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 5336225 5337332 5336225 ID=MS.gene30954
chr2.2 mRNA 5336225 5337332 5336225 ID=MS.gene30954.t1;Parent=MS.gene30954
chr2.2 exon 5337282 5337332 5337282 ID=MS.gene30954.t1.exon1;Parent=MS.gene30954.t1
chr2.2 CDS 5337282 5337332 5337282 ID=cds.MS.gene30954.t1;Parent=MS.gene30954.t1
chr2.2 exon 5336225 5337085 5336225 ID=MS.gene30954.t1.exon2;Parent=MS.gene30954.t1
chr2.2 CDS 5336225 5337085 5336225 ID=cds.MS.gene30954.t1;Parent=MS.gene30954.t1
Gene Sequence

>MS.gene30954

ATGGTTTTCTCTTCTATTCCATCTTATTTAGATCCTCTCAATTGGCAGCAAGTAAGTTTTTTCTTTTTCTTTTTTTTCCACAAAAGAGTGAATTTATTTATTCCTATTCCTTTGTAATAATAATATCTTCTTGAATATACCTGCAGATTTTTTTTCATTACAACTCAAGAATAATTTCATCTTTTTCCCTTTCTTGTAACCTTGTGATATATCTATATATGAATTTCATTATACATATGTTTTGCAGCAACCAAATCAACATCAGTTGCTTCCACCACTATCCTCATCACAGCCACATGGTGCAGGCTCAATTATCAGGCCCGGTTCGATACCGGATCAAGCTCAAGCTCAGGCAGCTCATGAAGTTCAAACTGGTCATCAGGTTCAAACTCACCAAGCTAAGTTACCACCACCTTCAGAGACAGCTTTGAAGTGTCCTCGTTGTGAATCAACCAACACAAAATTTTGTTACTACAACAACTATAACCTGTCTCAACCAAGACACTTTTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCATTAAGAAATGTTCCTGTTGGTGGAGGGTGTCGTAGAAGCAAAAAGACCAAAAAGAGTAACACCTCTACTCCCTCAAAATCTCCAATATCAAATAGTAATGAGAAAGATTATTCAACAAGTGCAATCCATTCAACTTCAACTCCTCTTGGTGAATTCTTACACCCAACTAATAGACCTAATTACATGACATCTCTTCAGAATCTGAACCGGTTCGGTGTTGGAAACATGTTTCAAATTGGTGATTATAGTTCAAGTACTGGTGGAGGAGACGGAGGAGTTGTGGATCAGTGGAGATTTCAGCAGTTTCCTTTCATGAATAATGGTTTTGATTCAAGTACTTCAAATGTTTCTTTCCCATTTCAAAGTGAAATTGTTGAACCAACAACTACTTCTAGGGTTACTACTTTGATGCCTGCATCGGTTAAATCGGAACACAACGGAGGAGGTTTTAATCTGCTTAGATCTCCAATGAATATGTCAGAGAATAACAACCATTACAATAATTCATCATGGACTGATTTTCATGGTCTTGCATCTTCTTCGGCTAGTCGTCTGTTATAA
Protein sequence

>MS.gene30954.t1

MVFSSIPSYLDPLNWQQQPNQHQLLPPLSSSQPHGAGSIIRPGSIPDQAQAQAAHEVQTGHQVQTHQAKLPPPSETALKCPRCESTNTKFCYYNNYNLSQPRHFCKTCRRYWTRGGALRNVPVGGGCRRSKKTKKSNTSTPSKSPISNSNEKDYSTSAIHSTSTPLGEFLHPTNRPNYMTSLQNLNRFGVGNMFQIGDYSSSTGGGDGGVVDQWRFQQFPFMNNGFDSSTSNVSFPFQSEIVEPTTTSRVTTLMPASVKSEHNGGGFNLLRSPMNMSENNNHYNNSSWTDFHGLASSSASRLL