AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021109

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021109.t1 MTR_4g063780 95.536 336 12 2 1 335 1 334 0.0 586
MS.gene021109.t1 MTR_2g096740 41.504 359 130 17 1 335 1 303 2.65e-63 203
MS.gene021109.t1 MTR_7g059400 43.465 329 136 10 1 311 1 297 1.16e-61 200
MS.gene021109.t1 MTR_2g096740 38.997 359 124 15 1 335 1 288 3.62e-57 187
MS.gene021109.t1 MTR_4g022370 38.968 349 154 12 1 316 1 323 1.48e-55 185
MS.gene021109.t1 MTR_7g024670 59.028 144 52 4 1 139 1 142 1.93e-46 161
MS.gene021109.t1 MTR_5g031440 87.500 56 7 0 70 125 38 93 1.32e-32 124
MS.gene021109.t1 MTR_3g077750 54.867 113 44 3 48 160 37 142 1.77e-32 124
MS.gene021109.t1 MTR_4g088580 42.197 173 64 5 76 221 56 219 2.93e-32 124
MS.gene021109.t1 MTR_2g093220 80.328 61 12 0 69 129 34 94 1.59e-31 120
MS.gene021109.t1 MTR_4g461080 66.667 78 25 1 50 127 5 81 1.80e-31 119
MS.gene021109.t1 MTR_8g479350 60.674 89 26 1 75 163 68 147 1.98e-31 121
MS.gene021109.t1 MTR_8g027295 57.732 97 39 1 62 158 11 105 1.14e-30 117
MS.gene021109.t1 MTR_8g068210 69.620 79 20 2 74 152 31 105 1.21e-30 119
MS.gene021109.t1 MTR_8g079060 54.717 106 32 3 54 159 17 106 7.88e-30 114
MS.gene021109.t1 MTR_3g091820 67.089 79 18 2 56 134 28 98 9.56e-30 115
MS.gene021109.t1 MTR_1g077600 60.000 80 30 1 52 129 18 97 3.02e-29 113
MS.gene021109.t1 MTR_1g077600 60.000 80 30 1 52 129 18 97 3.54e-29 114
MS.gene021109.t1 MTR_3g090430 63.514 74 27 0 56 129 32 105 1.02e-28 113
MS.gene021109.t1 MTR_8g015840 58.750 80 30 1 59 135 1 80 1.30e-28 110
MS.gene021109.t1 MTR_2g014060 67.606 71 22 1 57 126 6 76 2.16e-28 112
MS.gene021109.t1 MTR_4g109980 82.692 52 9 0 74 125 39 90 3.30e-28 112
MS.gene021109.t1 MTR_2g014170 88.000 50 6 0 74 123 44 93 5.65e-28 111
MS.gene021109.t1 MTR_1g056810 63.636 77 27 1 77 153 14 89 6.44e-28 110
MS.gene021109.t1 MTR_2g059540 84.000 50 8 0 74 123 30 79 8.92e-27 105
MS.gene021109.t1 MTR_4g089095 71.186 59 15 1 75 133 50 106 5.00e-25 102
MS.gene021109.t1 MTR_4g089095 71.186 59 15 1 75 133 16 72 6.05e-25 102
MS.gene021109.t1 MTR_2g013370 66.129 62 16 1 74 135 45 101 2.09e-24 100
MS.gene021109.t1 MTR_8g044220 66.102 59 20 0 71 129 114 172 2.86e-24 102
MS.gene021109.t1 MTR_2g016030 63.380 71 25 1 72 142 44 113 3.57e-24 97.1
MS.gene021109.t1 MTR_6g027450 63.333 60 22 0 68 127 82 141 7.41e-24 100
MS.gene021109.t1 MTR_6g012450 66.667 60 20 0 68 127 129 188 1.04e-23 101
MS.gene021109.t1 MTR_4g082060 58.571 70 26 1 58 127 108 174 1.72e-23 100
MS.gene021109.t1 MTR_7g010950 49.412 85 43 0 68 152 128 212 2.18e-23 100
MS.gene021109.t1 MTR_7g086780 60.000 70 25 2 60 129 86 152 2.24e-23 100
MS.gene021109.t1 MTR_3g435480 62.687 67 24 1 68 134 129 194 3.38e-23 100
MS.gene021109.t1 MTR_6g027460 61.667 60 23 0 68 127 89 148 4.59e-23 98.6
MS.gene021109.t1 MTR_5g041420 43.820 89 50 0 39 127 102 190 1.22e-21 94.0
MS.gene021109.t1 MTR_5g041530 69.091 55 16 1 77 131 142 195 1.61e-20 91.7
MS.gene021109.t1 MTR_5g041380 48.780 82 41 1 59 140 111 191 5.72e-20 90.1
MS.gene021109.t1 MTR_5g041400 50.725 69 34 0 59 127 103 171 1.23e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021109.t1 AT3G55370 38.636 352 138 13 1 323 1 303 8.80e-52 174
MS.gene021109.t1 AT3G55370 42.322 267 104 8 1 250 1 234 1.69e-51 174
MS.gene021109.t1 AT1G07640 42.456 285 145 9 57 334 3 275 5.49e-50 168
MS.gene021109.t1 AT1G07640 42.254 284 147 8 57 334 59 331 2.36e-49 168
MS.gene021109.t1 AT1G07640 42.254 284 147 8 57 334 67 339 3.31e-49 168
MS.gene021109.t1 AT3G55370 39.936 313 118 11 32 323 85 348 4.96e-49 168
MS.gene021109.t1 AT2G37590 53.846 156 32 2 1 129 1 143 8.34e-47 161
MS.gene021109.t1 AT2G28810 37.815 357 170 15 1 331 1 331 3.99e-46 160
MS.gene021109.t1 AT5G02460 88.158 76 9 0 54 129 74 149 4.83e-44 156
MS.gene021109.t1 AT2G28810 40.283 283 135 11 57 331 58 314 7.68e-43 150
MS.gene021109.t1 AT5G60200 76.389 72 13 1 70 141 50 117 1.82e-34 127
MS.gene021109.t1 AT1G51700 58.889 90 29 1 70 159 28 109 1.18e-31 117
MS.gene021109.t1 AT5G60850 62.791 86 25 2 74 159 50 128 2.55e-31 120
MS.gene021109.t1 AT3G45610 53.409 88 39 1 70 155 35 122 2.99e-31 118
MS.gene021109.t1 AT4G38000 77.049 61 14 0 69 129 35 95 2.24e-30 116
MS.gene021109.t1 AT5G65590 81.034 58 11 0 75 132 41 98 4.57e-30 117
MS.gene021109.t1 AT3G21270 53.000 100 28 2 75 155 29 128 3.81e-29 111
MS.gene021109.t1 AT4G24060 50.000 112 56 0 69 180 47 158 1.92e-28 113
MS.gene021109.t1 AT1G28310 79.661 59 11 1 75 133 27 84 3.68e-28 111
MS.gene021109.t1 AT3G52440 70.588 68 16 1 77 144 27 90 4.08e-28 110
MS.gene021109.t1 AT3G52440 70.588 68 16 1 77 144 46 109 4.97e-28 110
MS.gene021109.t1 AT2G28510 73.846 65 13 1 74 138 47 107 5.27e-28 110
MS.gene021109.t1 AT1G28310 79.661 59 11 1 75 133 51 108 5.73e-28 111
MS.gene021109.t1 AT3G61850 58.889 90 29 2 71 156 58 143 2.20e-27 108
MS.gene021109.t1 AT3G61850 58.889 90 29 2 71 156 58 143 2.20e-27 108
MS.gene021109.t1 AT5G66940 50.000 100 36 2 56 144 2 98 2.35e-27 107
MS.gene021109.t1 AT3G61850 58.889 90 29 2 71 156 70 155 3.08e-27 108
MS.gene021109.t1 AT3G61850 58.889 90 29 2 71 156 58 143 3.10e-27 109
MS.gene021109.t1 AT1G21340 73.770 61 10 1 77 137 40 94 3.43e-27 107
MS.gene021109.t1 AT3G61850 58.889 90 29 2 71 156 70 155 3.79e-27 109
MS.gene021109.t1 AT4G21050 47.581 124 50 3 68 190 17 126 4.35e-27 106
MS.gene021109.t1 AT3G50410 70.690 58 17 0 75 132 30 87 1.41e-26 106
MS.gene021109.t1 AT2G46590 56.250 80 35 0 43 122 36 115 3.08e-26 107
MS.gene021109.t1 AT2G46590 56.250 80 35 0 43 122 48 127 3.46e-26 107
MS.gene021109.t1 AT4G00940 63.514 74 23 2 59 129 50 122 1.21e-25 104
MS.gene021109.t1 AT4G00940 63.514 74 23 2 59 129 50 122 1.21e-25 104
MS.gene021109.t1 AT4G00940 63.514 74 23 2 59 129 50 122 1.21e-25 104
MS.gene021109.t1 AT1G64620 66.176 68 23 0 56 123 30 97 4.17e-25 104
MS.gene021109.t1 AT1G47655 70.000 60 16 1 66 123 18 77 1.99e-24 99.4
MS.gene021109.t1 AT2G34140 58.442 77 28 2 53 129 39 111 3.72e-24 97.4
MS.gene021109.t1 AT1G29160 69.492 59 17 1 71 129 58 115 1.13e-23 96.3
MS.gene021109.t1 AT4G21040 56.250 80 30 1 77 156 27 101 3.46e-23 96.3
MS.gene021109.t1 AT5G39660 66.667 63 20 1 67 129 130 191 3.81e-23 100
MS.gene021109.t1 AT5G39660 66.667 63 20 1 67 129 130 191 3.81e-23 100
MS.gene021109.t1 AT5G39660 66.667 63 20 1 67 129 130 191 3.81e-23 100
MS.gene021109.t1 AT1G26790 54.412 68 31 0 60 127 96 163 4.01e-23 99.0
MS.gene021109.t1 AT5G62430 56.000 75 31 2 58 131 36 109 4.19e-23 97.8
MS.gene021109.t1 AT3G47500 65.000 60 21 0 68 127 103 162 8.31e-23 99.0
MS.gene021109.t1 AT1G69570 61.667 60 23 0 68 127 125 184 2.21e-22 97.1
MS.gene021109.t1 AT4G21080 63.492 63 18 1 77 139 27 84 7.71e-22 93.2
MS.gene021109.t1 AT5G62940 36.464 181 77 4 69 213 67 245 2.36e-19 88.2
MS.gene021109.t1 AT4G21030 54.098 61 25 1 68 128 17 74 2.05e-15 73.9

Find 86 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGACATGTAGGCGTTAT+TGG 0.207030 4.4:-42297090 MS.gene021109:CDS
GTTGTTGTTGATGTTGTTCT+TGG 0.217968 4.4:+42296798 None:intergenic
TCAAGAGCCACCGGTTAAAA+TGG 0.246428 4.4:-42296528 MS.gene021109:CDS
TCTTGAGAGTTTGTTGAATT+AGG 0.247926 4.4:+42296980 None:intergenic
CCATTTGCTTGTTGATGATT+TGG 0.249048 4.4:+42297337 None:intergenic
ACCAACAACAATACTATTCT+TGG 0.250684 4.4:-42296448 MS.gene021109:CDS
GTTTCTGCTTCTGCTGTTGC+TGG 0.257390 4.4:-42296716 MS.gene021109:CDS
GATCATATGGGGTTTCAAAT+TGG 0.271163 4.4:-42296770 MS.gene021109:CDS
TATTCTTGGAATGATTTATC+AGG 0.272738 4.4:-42296434 MS.gene021109:CDS
TACTGGTGGCGGTGGTACTA+TGG 0.292815 4.4:-42297263 MS.gene021109:CDS
TTTACCTGGTGGTGCCAATT+AGG 0.304320 4.4:+42297540 None:intergenic
CCTTCTCATCATGAACTAAT+TGG 0.327970 4.4:-42296929 MS.gene021109:CDS
GTTATGTAGATCCTCCTAAT+TGG 0.350822 4.4:-42297554 MS.gene021109:CDS
AACATTGAAGCTGCAGCATC+TGG 0.352153 4.4:-42296587 MS.gene021109:CDS
AATTCCTAACCTACCCTTCA+TGG 0.365060 4.4:-42296888 MS.gene021109:CDS
ACAACAACAACAAGATCATA+TGG 0.370266 4.4:-42296783 MS.gene021109:CDS
TCTGCTGTTGCTGGAGGTGT+TGG 0.398368 4.4:-42296707 MS.gene021109:CDS
TTTCAAATTGGTATTGGTAA+TGG 0.399190 4.4:-42296758 MS.gene021109:CDS
TCCAAGAATAGTATTGTTGT+TGG 0.406762 4.4:+42296447 None:intergenic
ATGGGGTTTCAAATTGGTAT+TGG 0.408013 4.4:-42296764 MS.gene021109:CDS
CTACGACACCCTCCACCAAC+TGG 0.417773 4.4:+42297040 None:intergenic
GGACACTTTAGCGCTGGTTC+TGG 0.424523 4.4:+42297184 None:intergenic
TGAGAAGGGATTGCACTTGA+TGG 0.431346 4.4:+42296944 None:intergenic
GCTCGATCAGCCATGGAGCC+TGG 0.444337 4.4:+42297229 None:intergenic
CCTCAAGTGGGAGGTAGTAC+TGG 0.448394 4.4:-42297280 MS.gene021109:CDS
CCAAATCATCAACAAGCAAA+TGG 0.450966 4.4:-42297337 MS.gene021109:CDS
GCTGCAGCATCTGGATTTGT+TGG 0.454209 4.4:-42296578 MS.gene021109:CDS
GATTCAAAAGTATTCAAGAA+AGG 0.460724 4.4:+42296653 None:intergenic
CTTAGAAATGTTCCAGTTGG+TGG 0.472325 4.4:-42297052 MS.gene021109:CDS
CTCGATCAGACCAGGCTCCA+TGG 0.475078 4.4:-42297239 MS.gene021109:CDS
GAGATATAGCTTCAAACTCT+AGG 0.480948 4.4:-42296556 MS.gene021109:CDS
CCTCCCACTTGAGGTGGTAA+AGG 0.483237 4.4:+42297289 None:intergenic
TTTAGCGCTGGTTCTGGTGG+TGG 0.483860 4.4:+42297190 None:intergenic
GGTAGGTTAGGAATTGTTTG+TGG 0.484498 4.4:+42296896 None:intergenic
CAACAACAACAAGATCATAT+GGG 0.484986 4.4:-42296782 MS.gene021109:CDS
GAGGCCATGAAGGGTAGGTT+AGG 0.490552 4.4:+42296884 None:intergenic
AGAAATGTTCCAGTTGGTGG+AGG 0.497986 4.4:-42297049 MS.gene021109:CDS
CCAATTAGTTCATGATGAGA+AGG 0.516486 4.4:+42296929 None:intergenic
CACTTTAGCGCTGGTTCTGG+TGG 0.522746 4.4:+42297187 None:intergenic
GAAGTAGCAAAATTTAGTGT+TGG 0.523027 4.4:+42297147 None:intergenic
TCCTCCTAATTGGCACCACC+AGG 0.528426 4.4:-42297544 MS.gene021109:intron
ACTGGTGGCGGTGGTACTAT+GGG 0.530847 4.4:-42297262 MS.gene021109:CDS
GGAAACGTTAACATGGGACT+TGG 0.531022 4.4:-42296833 MS.gene021109:CDS
GAGAAGGGATTGCACTTGAT+GGG 0.532889 4.4:+42296945 None:intergenic
AATCTATCAAGAACATCCCT+TGG 0.539894 4.4:-42296488 MS.gene021109:CDS
CAACGAGGACACTTTAGCGC+TGG 0.540252 4.4:+42297178 None:intergenic
ACCTGGTGGTGCCAATTAGG+AGG 0.541280 4.4:+42297543 None:intergenic
TCTGCTTCTGCTGTTGCTGG+AGG 0.548891 4.4:-42296713 MS.gene021109:CDS
AGATATAGCTTCAAACTCTA+GGG 0.556521 4.4:-42296555 MS.gene021109:CDS
GGAGGTAGTACTGGTGGCGG+TGG 0.560481 4.4:-42297271 MS.gene021109:CDS
ATTCTGCATCGAGGCCATGA+AGG 0.561082 4.4:+42296874 None:intergenic
AATCTGAGTCGTTATGTCGT+TGG 0.570962 4.4:-42296854 MS.gene021109:CDS
CAATTAGTTCATGATGAGAA+GGG 0.573984 4.4:+42296930 None:intergenic
TGTCGTTGGAAACGTTAACA+TGG 0.575317 4.4:-42296840 MS.gene021109:CDS
TCCACCTTTACCACCTCAAG+TGG 0.577272 4.4:-42297293 MS.gene021109:CDS
ACTATGGGCTCGATCAGACC+AGG 0.580839 4.4:-42297247 MS.gene021109:CDS
TTCTGCATCGAGGCCATGAA+GGG 0.581643 4.4:+42296875 None:intergenic
CCCACTTGAGGTGGTAAAGG+TGG 0.581750 4.4:+42297292 None:intergenic
GAAATGTTCCAGTTGGTGGA+GGG 0.594932 4.4:-42297048 MS.gene021109:CDS
GCACTTAGAAATGTTCCAGT+TGG 0.600640 4.4:-42297055 MS.gene021109:CDS
AGAAGGGATTGCACTTGATG+GGG 0.604506 4.4:+42296946 None:intergenic
GTAGGCGTTATTGGACAAGA+GGG 0.605161 4.4:-42297081 MS.gene021109:CDS
TAGGGCTAATCAAGAGCCAC+CGG 0.605644 4.4:-42296537 MS.gene021109:CDS
GTCGTTGGAAACGTTAACAT+GGG 0.609381 4.4:-42296839 MS.gene021109:CDS
GTCACTTCTGCAAGACATGT+AGG 0.611130 4.4:-42297099 MS.gene021109:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 4.4:-42297082 MS.gene021109:CDS
GCATCGAGGCCATGAAGGGT+AGG 0.623817 4.4:+42296879 None:intergenic
AATTGCTGCAAATTACGCCA+TGG 0.629104 4.4:+42296677 None:intergenic
TAAACGAGCTCGATCAGCCA+TGG 0.633447 4.4:+42297222 None:intergenic
CATGTCTTGCAGAAGTGACG+AGG 0.635723 4.4:+42297103 None:intergenic
GTGGGAGGTAGTACTGGTGG+CGG 0.638124 4.4:-42297274 MS.gene021109:CDS
AACAACAACAAGATCATATG+GGG 0.646211 4.4:-42296781 MS.gene021109:CDS
TATTATGTTCAGAGACACCA+AGG 0.647047 4.4:+42296471 None:intergenic
CCACCTTTACCACCTCAAGT+GGG 0.662217 4.4:-42297292 MS.gene021109:CDS
CAAGTGGGAGGTAGTACTGG+TGG 0.664836 4.4:-42297277 MS.gene021109:CDS
GTACTACCTCCCACTTGAGG+TGG 0.666584 4.4:+42297283 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 4.4:-42297080 MS.gene021109:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 4.4:-42297079 MS.gene021109:CDS
CCAGTACTACCTCCCACTTG+AGG 0.681370 4.4:+42297280 None:intergenic
CCTTTACCACCTCAAGTGGG+AGG 0.700568 4.4:-42297289 MS.gene021109:CDS
GAGGTGTTGGAGTTGAACCA+TGG 0.701694 4.4:-42296694 MS.gene021109:CDS
TTGAATAGAAGAGAAAACCA+TGG 0.707467 4.4:+42297578 None:intergenic
ATTATGTTCAGAGACACCAA+GGG 0.718424 4.4:+42296472 None:intergenic
AGAAGTGACGAGGTTGAGTG+AGG 0.724186 4.4:+42297113 None:intergenic
GTGTTGGTTGAATCGCAACG+AGG 0.733913 4.4:+42297163 None:intergenic
ACGACTCAGATTCTGCATCG+AGG 0.741695 4.4:+42296865 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAAAAAATTATTGTGA+TGG + chr4.4:42296585-42296604 None:intergenic 10.0%
!! ATGGAAAGAAATTAAAAAAA+GGG + chr4.4:42296609-42296628 None:intergenic 15.0%
!! ATGAAAATTAAAACTTTACC+TGG + chr4.4:42296462-42296481 None:intergenic 20.0%
!! GATGGAAAGAAATTAAAAAA+AGG + chr4.4:42296610-42296629 None:intergenic 20.0%
!!! AATAATTCATACACTTTTCA+TGG - chr4.4:42297368-42297387 MS.gene021109:intron 20.0%
!!! TTCACTTTCTTTTTTGAATT+TGG + chr4.4:42296491-42296510 None:intergenic 20.0%
! AAAATTAAAACTTTACCTGG+TGG + chr4.4:42296459-42296478 None:intergenic 25.0%
! ATATTCAAGAAGATGAAGTA+AGG + chr4.4:42296549-42296568 None:intergenic 25.0%
! TATTCTTGGAATGATTTATC+AGG - chr4.4:42297551-42297570 MS.gene021109:CDS 25.0%
!! TTGAATTAGGAGATTTTGAA+CGG + chr4.4:42296995-42297014 None:intergenic 25.0%
!!! GATTCAAAAGTATTCAAGAA+AGG + chr4.4:42297335-42297354 None:intergenic 25.0%
!!! TTTCAAATTGGTATTGGTAA+TGG - chr4.4:42297227-42297246 MS.gene021109:CDS 25.0%
AACAACAACAAGATCATATG+GGG - chr4.4:42297204-42297223 MS.gene021109:CDS 30.0%
ACAACAACAACAAGATCATA+TGG - chr4.4:42297202-42297221 MS.gene021109:CDS 30.0%
ACCAACAACAATACTATTCT+TGG - chr4.4:42297537-42297556 MS.gene021109:intron 30.0%
AGATATAGCTTCAAACTCTA+GGG - chr4.4:42297430-42297449 MS.gene021109:intron 30.0%
CAACAACAACAAGATCATAT+GGG - chr4.4:42297203-42297222 MS.gene021109:CDS 30.0%
CAATTAGTTCATGATGAGAA+GGG + chr4.4:42297058-42297077 None:intergenic 30.0%
GAAGTAGCAAAATTTAGTGT+TGG + chr4.4:42296841-42296860 None:intergenic 30.0%
TCCAAGAATAGTATTGTTGT+TGG + chr4.4:42297541-42297560 None:intergenic 30.0%
! ATGGTTTTCTCTTCTATTCA+AGG - chr4.4:42296409-42296428 MS.gene021109:CDS 30.0%
!! CGATTTTCTTCCATTTTAAC+CGG + chr4.4:42297470-42297489 None:intergenic 30.0%
!! TCTTGAGAGTTTGTTGAATT+AGG + chr4.4:42297008-42297027 None:intergenic 30.0%
AATCTATCAAGAACATCCCT+TGG - chr4.4:42297497-42297516 MS.gene021109:intron 35.0%
ATGGGGTTTCAAATTGGTAT+TGG - chr4.4:42297221-42297240 MS.gene021109:CDS 35.0%
ATTATGTTCAGAGACACCAA+GGG + chr4.4:42297516-42297535 None:intergenic 35.0%
CCAAATCATCAACAAGCAAA+TGG - chr4.4:42296648-42296667 MS.gene021109:CDS 35.0%
CCAATTAGTTCATGATGAGA+AGG + chr4.4:42297059-42297078 None:intergenic 35.0%
CCTTCTCATCATGAACTAAT+TGG - chr4.4:42297056-42297075 MS.gene021109:CDS 35.0%
GAGATATAGCTTCAAACTCT+AGG - chr4.4:42297429-42297448 MS.gene021109:intron 35.0%
GATCATATGGGGTTTCAAAT+TGG - chr4.4:42297215-42297234 MS.gene021109:CDS 35.0%
GTTATGTAGATCCTCCTAAT+TGG - chr4.4:42296431-42296450 MS.gene021109:CDS 35.0%
TATTATGTTCAGAGACACCA+AGG + chr4.4:42297517-42297536 None:intergenic 35.0%
TTGCTGCAGGTTAAAATAGA+TGG + chr4.4:42296628-42296647 None:intergenic 35.0%
! ACTTTTGAATCAAACTCTGC+TGG - chr4.4:42297344-42297363 MS.gene021109:intron 35.0%
! GTTGTTGTTGATGTTGTTCT+TGG + chr4.4:42297190-42297209 None:intergenic 35.0%
!! CCATTTGCTTGTTGATGATT+TGG + chr4.4:42296651-42296670 None:intergenic 35.0%
!! TTTTCTTCCATTTTAACCGG+TGG + chr4.4:42297467-42297486 None:intergenic 35.0%
AATCTGAGTCGTTATGTCGT+TGG - chr4.4:42297131-42297150 MS.gene021109:CDS 40.0%
AATTCCTAACCTACCCTTCA+TGG - chr4.4:42297097-42297116 MS.gene021109:CDS 40.0%
AATTGCTGCAAATTACGCCA+TGG + chr4.4:42297311-42297330 None:intergenic 40.0%
CTTAGAAATGTTCCAGTTGG+TGG - chr4.4:42296933-42296952 MS.gene021109:CDS 40.0%
GCACTTAGAAATGTTCCAGT+TGG - chr4.4:42296930-42296949 MS.gene021109:CDS 40.0%
GGTAGGTTAGGAATTGTTTG+TGG + chr4.4:42297092-42297111 None:intergenic 40.0%
GTCGTTGGAAACGTTAACAT+GGG - chr4.4:42297146-42297165 MS.gene021109:CDS 40.0%
TGTCGTTGGAAACGTTAACA+TGG - chr4.4:42297145-42297164 MS.gene021109:CDS 40.0%
AACATTGAAGCTGCAGCATC+TGG - chr4.4:42297398-42297417 MS.gene021109:intron 45.0%
AGAAGGGATTGCACTTGATG+GGG + chr4.4:42297042-42297061 None:intergenic 45.0%
GAGAAGGGATTGCACTTGAT+GGG + chr4.4:42297043-42297062 None:intergenic 45.0%
GCAAGACATGTAGGCGTTAT+TGG - chr4.4:42296895-42296914 MS.gene021109:CDS 45.0%
GGAAACGTTAACATGGGACT+TGG - chr4.4:42297152-42297171 MS.gene021109:CDS 45.0%
GTAGGCGTTATTGGACAAGA+GGG - chr4.4:42296904-42296923 MS.gene021109:CDS 45.0%
GTCACTTCTGCAAGACATGT+AGG - chr4.4:42296886-42296905 MS.gene021109:CDS 45.0%
TAGGCGTTATTGGACAAGAG+GGG - chr4.4:42296905-42296924 MS.gene021109:CDS 45.0%
TGAGAAGGGATTGCACTTGA+TGG + chr4.4:42297044-42297063 None:intergenic 45.0%
TGTAGGCGTTATTGGACAAG+AGG - chr4.4:42296903-42296922 MS.gene021109:CDS 45.0%
! TCAAGAGCCACCGGTTAAAA+TGG - chr4.4:42297457-42297476 MS.gene021109:intron 45.0%
! TTTACCTGGTGGTGCCAATT+AGG + chr4.4:42296448-42296467 None:intergenic 45.0%
!! AGAAATGTTCCAGTTGGTGG+AGG - chr4.4:42296936-42296955 MS.gene021109:CDS 45.0%
!! GAAATGTTCCAGTTGGTGGA+GGG - chr4.4:42296937-42296956 MS.gene021109:CDS 45.0%
!! GTTGATGATTTGGTTGCTGC+AGG + chr4.4:42296641-42296660 None:intergenic 45.0%
ACGACTCAGATTCTGCATCG+AGG + chr4.4:42297123-42297142 None:intergenic 50.0%
AGAAGTGACGAGGTTGAGTG+AGG + chr4.4:42296875-42296894 None:intergenic 50.0%
AGGCGTTATTGGACAAGAGG+GGG - chr4.4:42296906-42296925 MS.gene021109:CDS 50.0%
ATTCTGCATCGAGGCCATGA+AGG + chr4.4:42297114-42297133 None:intergenic 50.0%
CATGTCTTGCAGAAGTGACG+AGG + chr4.4:42296885-42296904 None:intergenic 50.0%
CCACCTTTACCACCTCAAGT+GGG - chr4.4:42296693-42296712 MS.gene021109:CDS 50.0%
GCTGCAGCATCTGGATTTGT+TGG - chr4.4:42297407-42297426 MS.gene021109:intron 50.0%
GTTTCTGCTTCTGCTGTTGC+TGG - chr4.4:42297269-42297288 MS.gene021109:CDS 50.0%
TAAACGAGCTCGATCAGCCA+TGG + chr4.4:42296766-42296785 None:intergenic 50.0%
TAGGGCTAATCAAGAGCCAC+CGG - chr4.4:42297448-42297467 MS.gene021109:intron 50.0%
TCCACCTTTACCACCTCAAG+TGG - chr4.4:42296692-42296711 MS.gene021109:CDS 50.0%
TTCTGCATCGAGGCCATGAA+GGG + chr4.4:42297113-42297132 None:intergenic 50.0%
!! GAGGTGTTGGAGTTGAACCA+TGG - chr4.4:42297291-42297310 MS.gene021109:CDS 50.0%
!! GTGTTGGTTGAATCGCAACG+AGG + chr4.4:42296825-42296844 None:intergenic 50.0%
ACTATGGGCTCGATCAGACC+AGG - chr4.4:42296738-42296757 MS.gene021109:CDS 55.0%
CAACGAGGACACTTTAGCGC+TGG + chr4.4:42296810-42296829 None:intergenic 55.0%
CCAGTACTACCTCCCACTTG+AGG + chr4.4:42296708-42296727 None:intergenic 55.0%
CCCACTTGAGGTGGTAAAGG+TGG + chr4.4:42296696-42296715 None:intergenic 55.0%
CCTCAAGTGGGAGGTAGTAC+TGG - chr4.4:42296705-42296724 MS.gene021109:CDS 55.0%
CCTCCCACTTGAGGTGGTAA+AGG + chr4.4:42296699-42296718 None:intergenic 55.0%
CCTTTACCACCTCAAGTGGG+AGG - chr4.4:42296696-42296715 MS.gene021109:CDS 55.0%
GAGGCCATGAAGGGTAGGTT+AGG + chr4.4:42297104-42297123 None:intergenic 55.0%
GTACTACCTCCCACTTGAGG+TGG + chr4.4:42296705-42296724 None:intergenic 55.0%
TCCTCCTAATTGGCACCACC+AGG - chr4.4:42296441-42296460 MS.gene021109:CDS 55.0%
TCTGCTTCTGCTGTTGCTGG+AGG - chr4.4:42297272-42297291 MS.gene021109:CDS 55.0%
! ACCTGGTGGTGCCAATTAGG+AGG + chr4.4:42296445-42296464 None:intergenic 55.0%
! CAAGTGGGAGGTAGTACTGG+TGG - chr4.4:42296708-42296727 MS.gene021109:CDS 55.0%
! TCTGCTGTTGCTGGAGGTGT+TGG - chr4.4:42297278-42297297 MS.gene021109:CDS 55.0%
!! ACTGGTGGCGGTGGTACTAT+GGG - chr4.4:42296723-42296742 MS.gene021109:CDS 55.0%
!! CACTTTAGCGCTGGTTCTGG+TGG + chr4.4:42296801-42296820 None:intergenic 55.0%
!! GGACACTTTAGCGCTGGTTC+TGG + chr4.4:42296804-42296823 None:intergenic 55.0%
!! TACTGGTGGCGGTGGTACTA+TGG - chr4.4:42296722-42296741 MS.gene021109:CDS 55.0%
!! TTTAGCGCTGGTTCTGGTGG+TGG + chr4.4:42296798-42296817 None:intergenic 55.0%
CTACGACACCCTCCACCAAC+TGG + chr4.4:42296948-42296967 None:intergenic 60.0%
CTCGATCAGACCAGGCTCCA+TGG - chr4.4:42296746-42296765 MS.gene021109:CDS 60.0%
GCATCGAGGCCATGAAGGGT+AGG + chr4.4:42297109-42297128 None:intergenic 60.0%
!! GTGGGAGGTAGTACTGGTGG+CGG - chr4.4:42296711-42296730 MS.gene021109:CDS 60.0%
GCTCGATCAGCCATGGAGCC+TGG + chr4.4:42296759-42296778 None:intergenic 65.0%
!! GGAGGTAGTACTGGTGGCGG+TGG - chr4.4:42296714-42296733 MS.gene021109:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 42296409 42297598 42296409 ID=MS.gene021109
chr4.4 mRNA 42296409 42297598 42296409 ID=MS.gene021109.t1;Parent=MS.gene021109
chr4.4 exon 42297545 42297598 42297545 ID=MS.gene021109.t1.exon1;Parent=MS.gene021109.t1
chr4.4 CDS 42297545 42297598 42297545 ID=cds.MS.gene021109.t1;Parent=MS.gene021109.t1
chr4.4 exon 42296409 42297362 42296409 ID=MS.gene021109.t1.exon2;Parent=MS.gene021109.t1
chr4.4 CDS 42296409 42297362 42296409 ID=cds.MS.gene021109.t1;Parent=MS.gene021109.t1
Gene Sequence

>MS.gene021109

ATGGTTTTCTCTTCTATTCAAGGTTATGTAGATCCTCCTAATTGGCACCACCAGGTAAAGTTTTAATTTTCATCAAATTCCAAATTCAAAAAAGAAAGTGAAAAAAAAAAACTTCAAAAAGATGAATCATTCATATTCCTTACTTCATCTTCTTGAATATACTAATTTTTTTTCCATCACAATAATTTTTTTAAATTCCCTTTTTTTAATTTCTTTCCATCTATTTTAACCTGCAGCAACCAAATCATCAACAAGCAAATGGAAGCAATAACACTCAACTTCTTCCACCTTTACCACCTCAAGTGGGAGGTAGTACTGGTGGCGGTGGTACTATGGGCTCGATCAGACCAGGCTCCATGGCTGATCGAGCTCGTTTAGCAAAACTACCACCACCAGAACCAGCGCTAAAGTGTCCTCGTTGCGATTCAACCAACACTAAATTTTGCTACTTCAACAACTACAGCCTCACTCAACCTCGTCACTTCTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCACTTAGAAATGTTCCAGTTGGTGGAGGGTGTCGTAGAAACAACAAGAAAAACAAAAGAAGCCGTTCAAAATCTCCTAATTCAACAAACTCTCAAGAAAAACAAACTTCCCCATCAAGTGCAATCCCTTCTCATCATGAACTAATTGGTCATCAGTTACCACAAACAATTCCTAACCTACCCTTCATGGCCTCGATGCAGAATCTGAGTCGTTATGTCGTTGGAAACGTTAACATGGGACTTGGTTTGCGTGAGATCCAAGAACAACATCAACAACAACAACAAGATCATATGGGGTTTCAAATTGGTATTGGTAATGGTAACTCAACTTCTGTTTCTGTTTCTGCTTCTGCTGTTGCTGGAGGTGTTGGAGTTGAACCATGGCGTAATTTGCAGCAATTTCCTTTCTTGAATACTTTTGAATCAAACTCTGCTGGAAATAATTCATACACTTTTCATGGAGAAAGTAACATTGAAGCTGCAGCATCTGGATTTGTTGGAGATATAGCTTCAAACTCTAGGGCTAATCAAGAGCCACCGGTTAAAATGGAAGAAAATCGAGCTTTAAATCTATCAAGAACATCCCTTGGTGTCTCTGAACATAATAACCAACAACAATACTATTCTTGGAATGATTTATCAGGCTCTTCAACTACTCATCTCTTATAA
Protein sequence

>MS.gene021109.t1

MVFSSIQGYVDPPNWHHQQPNHQQANGSNNTQLLPPLPPQVGGSTGGGGTMGSIRPGSMADRARLAKLPPPEPALKCPRCDSTNTKFCYFNNYSLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKKNKRSRSKSPNSTNSQEKQTSPSSAIPSHHELIGHQLPQTIPNLPFMASMQNLSRYVVGNVNMGLGLREIQEQHQQQQQDHMGFQIGIGNGNSTSVSVSASAVAGGVGVEPWRNLQQFPFLNTFESNSAGNNSYTFHGESNIEAAASGFVGDIASNSRANQEPPVKMEENRALNLSRTSLGVSEHNNQQQYYSWNDLSGSSTTHLL