AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004434

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004434.t1 MTR_2g019640 96.311 515 19 0 3 517 1 515 0.0 1033
MS.gene004434.t1 MTR_6g034940 24.894 470 302 16 15 463 5 444 3.10e-28 117
MS.gene004434.t1 MTR_5g025610 24.950 497 313 19 21 499 6 460 5.75e-26 110
MS.gene004434.t1 MTR_2g031920 24.118 510 329 19 22 504 11 489 7.30e-26 110
MS.gene004434.t1 MTR_5g025610 25.322 466 290 18 21 468 6 431 1.42e-25 109
MS.gene004434.t1 MTR_2g031930 24.689 482 314 19 39 504 41 489 3.57e-25 108
MS.gene004434.t1 MTR_0045s0070 25.773 485 298 16 37 501 34 476 2.52e-23 103
MS.gene004434.t1 MTR_8g089800 22.669 472 324 16 38 495 38 482 5.97e-23 102
MS.gene004434.t1 MTR_3g467130 24.153 443 273 15 38 455 28 432 1.25e-22 101
MS.gene004434.t1 MTR_8g072260 23.168 505 305 15 25 502 17 465 1.38e-22 100
MS.gene004434.t1 MTR_8g072260 23.779 471 278 14 25 468 17 433 2.74e-22 100
MS.gene004434.t1 MTR_3g467140 22.817 504 343 16 18 504 5 479 4.28e-22 99.8
MS.gene004434.t1 MTR_2g031930 24.887 442 290 16 39 466 37 450 5.24e-22 99.0
MS.gene004434.t1 MTR_0045s0060 23.156 488 297 18 42 501 39 476 1.80e-21 97.8
MS.gene004434.t1 MTR_0045s0060 24.051 395 249 14 120 501 38 394 1.87e-21 96.7
MS.gene004434.t1 MTR_0045s0070 25.951 447 271 15 37 463 34 440 3.89e-21 96.3
MS.gene004434.t1 MTR_5g014250 23.013 478 322 15 34 495 36 483 6.84e-21 95.9
MS.gene004434.t1 MTR_1g019410 24.506 506 310 19 25 505 10 468 1.37e-20 95.1
MS.gene004434.t1 MTR_4g086040 25.959 443 275 17 40 467 35 439 1.38e-20 95.1
MS.gene004434.t1 MTR_4g095050 26.493 268 165 10 221 479 232 476 2.00e-20 94.7
MS.gene004434.t1 MTR_4g086130 24.661 442 282 16 40 467 32 436 1.46e-19 91.7
MS.gene004434.t1 MTR_6g015910 22.245 481 320 16 21 489 13 451 2.20e-19 91.3
MS.gene004434.t1 MTR_5g010750 23.182 440 283 17 43 463 31 434 2.21e-19 91.3
MS.gene004434.t1 MTR_5g010750 23.182 440 283 17 43 463 31 434 3.81e-19 90.5
MS.gene004434.t1 MTR_8g076940 23.647 499 310 21 25 491 20 479 4.62e-19 90.5
MS.gene004434.t1 MTR_2g437840 26.429 280 171 13 228 497 235 489 4.87e-19 90.5
MS.gene004434.t1 MTR_8g072260 24.065 428 249 13 25 425 17 395 7.75e-19 89.7
MS.gene004434.t1 MTR_3g007320 22.667 450 307 14 21 463 13 428 9.42e-19 89.4
MS.gene004434.t1 MTR_5g091760 25.571 438 277 15 40 461 35 439 9.97e-19 89.4
MS.gene004434.t1 MTR_7g016040 22.874 494 324 20 20 500 12 461 2.55e-18 87.8
MS.gene004434.t1 MTR_3g112120 22.840 486 315 16 19 478 10 461 9.20e-18 86.3
MS.gene004434.t1 MTR_5g014240 23.950 476 319 14 34 495 36 482 1.41e-17 85.9
MS.gene004434.t1 MTR_4g102280 27.419 248 167 8 228 472 240 477 2.23e-17 85.5
MS.gene004434.t1 MTR_8g006495 31.548 168 107 5 295 455 158 324 2.43e-17 84.3
MS.gene004434.t1 MTR_0045s0080 25.559 313 197 9 120 426 120 402 5.30e-17 83.6
MS.gene004434.t1 MTR_4g094775 26.316 228 146 7 257 481 260 468 6.75e-17 84.0
MS.gene004434.t1 MTR_5g016440 26.697 221 147 6 274 491 267 475 8.50e-17 83.6
MS.gene004434.t1 MTR_0059s0170 29.612 206 135 5 296 494 292 494 9.34e-17 83.6
MS.gene004434.t1 MTR_8g006450 31.548 168 107 5 295 455 271 437 1.05e-16 83.2
MS.gene004434.t1 MTR_4g102310 25.806 248 170 7 228 472 235 471 1.40e-15 79.7
MS.gene004434.t1 MTR_3g467140 26.384 307 196 10 208 504 60 346 2.05e-15 78.2
MS.gene004434.t1 MTR_1g111240 32.948 173 103 6 310 473 337 505 3.55e-15 78.6
MS.gene004434.t1 MTR_7g033040 22.650 468 310 15 14 461 4 439 1.42e-14 76.6
MS.gene004434.t1 MTR_3g058060 25.000 252 163 8 253 494 261 496 2.41e-14 76.3
MS.gene004434.t1 MTR_1g032950 20.325 492 330 20 23 495 19 467 2.83e-14 75.5
MS.gene004434.t1 MTR_0171s0010 26.244 221 152 7 257 473 264 477 2.91e-14 75.5
MS.gene004434.t1 MTR_3g058220 25.962 208 144 5 294 494 289 493 2.93e-14 75.5
MS.gene004434.t1 MTR_6g022450 26.549 226 148 7 263 473 257 479 4.01e-14 75.1
MS.gene004434.t1 MTR_4g077210 29.341 167 111 5 292 455 289 451 4.46e-14 75.1
MS.gene004434.t1 MTR_7g079440 27.717 184 121 5 296 473 442 619 4.86e-14 75.5
MS.gene004434.t1 MTR_7g006900 30.964 197 122 7 307 499 283 469 5.66e-14 74.7
MS.gene004434.t1 MTR_7g012860 28.022 182 122 5 296 473 291 467 5.95e-14 74.7
MS.gene004434.t1 MTR_0287s0060 28.022 182 122 5 296 473 291 467 5.95e-14 74.7
MS.gene004434.t1 MTR_6g017325 29.082 196 127 5 307 499 318 504 9.78e-14 73.9
MS.gene004434.t1 MTR_8g012585 22.957 257 166 6 263 494 191 440 1.12e-13 73.6
MS.gene004434.t1 MTR_1g035140 25.556 180 129 3 296 473 291 467 1.80e-13 73.2
MS.gene004434.t1 MTR_4g104610 31.138 167 107 5 300 461 295 458 1.90e-13 73.2
MS.gene004434.t1 MTR_5g095220 28.235 170 117 3 306 473 271 437 2.05e-13 72.8
MS.gene004434.t1 MTR_2g072310 25.292 257 163 9 230 473 83 323 2.12e-13 72.0
MS.gene004434.t1 MTR_4g062500 27.368 190 129 4 296 479 299 485 2.50e-13 72.8
MS.gene004434.t1 MTR_4g088160 24.153 236 155 6 276 502 274 494 3.09e-13 72.4
MS.gene004434.t1 MTR_5g095290 27.811 169 117 3 307 473 302 467 3.14e-13 72.4
MS.gene004434.t1 MTR_8g042060 25.442 283 182 11 232 501 221 487 3.88e-13 72.0
MS.gene004434.t1 MTR_4g104640 22.388 201 146 5 276 473 291 484 4.16e-13 72.0
MS.gene004434.t1 MTR_5g009110 26.522 230 136 6 304 503 345 571 4.24e-13 72.0
MS.gene004434.t1 MTR_5g009110 26.522 230 136 6 304 503 197 423 4.47e-13 71.6
MS.gene004434.t1 MTR_5g021220 21.825 504 329 20 20 489 4 476 4.58e-13 71.6
MS.gene004434.t1 MTR_8g036185 25.789 190 126 5 296 473 299 485 5.08e-13 71.6
MS.gene004434.t1 MTR_8g042060 26.070 257 163 9 257 501 282 523 6.49e-13 71.2
MS.gene004434.t1 MTR_5g007450 28.796 191 124 5 299 483 290 474 8.16e-13 70.9
MS.gene004434.t1 MTR_5g082520 21.830 481 322 16 39 503 24 466 1.05e-12 70.5
MS.gene004434.t1 MTR_4g104540 31.138 167 107 5 300 461 225 388 1.07e-12 70.5
MS.gene004434.t1 MTR_2g065360 26.257 179 126 4 298 473 264 439 1.41e-12 70.1
MS.gene004434.t1 MTR_4g104660 24.873 197 142 4 296 489 303 496 1.47e-12 70.1
MS.gene004434.t1 MTR_4g088195 24.155 207 141 5 276 473 274 473 1.64e-12 70.1
MS.gene004434.t1 MTR_5g018980 25.888 197 130 5 276 466 280 466 1.68e-12 70.1
MS.gene004434.t1 MTR_3g093530 28.235 170 115 3 306 472 330 495 1.97e-12 70.1
MS.gene004434.t1 MTR_0638s0020 25.140 179 128 4 298 473 221 396 2.10e-12 69.3
MS.gene004434.t1 MTR_8g012590 25.000 252 171 8 228 473 229 468 2.20e-12 69.7
MS.gene004434.t1 MTR_8g091680 26.667 180 122 6 299 473 244 418 2.71e-12 69.3
MS.gene004434.t1 MTR_3g057980 23.858 197 144 4 280 473 270 463 3.18e-12 69.3
MS.gene004434.t1 MTR_4g026030 28.824 170 111 6 300 464 138 302 3.30e-12 68.2
MS.gene004434.t1 MTR_4g062510 24.737 190 134 4 296 479 303 489 4.20e-12 68.9
MS.gene004434.t1 MTR_7g012330 27.848 158 107 3 296 451 292 444 4.59e-12 68.6
MS.gene004434.t1 MTR_8g042020 26.733 202 132 6 307 501 330 522 4.98e-12 68.6
MS.gene004434.t1 MTR_3g058000 25.112 223 152 6 254 473 262 472 5.15e-12 68.6
MS.gene004434.t1 MTR_6g008530 26.733 202 139 6 275 471 297 494 6.51e-12 68.2
MS.gene004434.t1 MTR_8g076290 26.570 207 133 8 257 453 255 452 7.14e-12 68.2
MS.gene004434.t1 MTR_3g076560 25.414 181 129 4 296 473 305 482 8.04e-12 67.8
MS.gene004434.t1 MTR_1g102220 26.512 215 124 7 284 473 272 477 8.19e-12 67.8
MS.gene004434.t1 MTR_4g026050 23.926 489 316 18 14 473 2 463 8.59e-12 67.8
MS.gene004434.t1 MTR_8g063280 26.230 183 128 4 297 473 286 467 9.25e-12 67.8
MS.gene004434.t1 MTR_5g007460 28.796 191 124 5 299 483 269 453 9.32e-12 67.8
MS.gene004434.t1 MTR_5g018990 25.989 177 119 6 296 466 294 464 1.09e-11 67.4
MS.gene004434.t1 MTR_3g070380 22.981 483 306 16 43 502 29 468 1.15e-11 67.4
MS.gene004434.t1 MTR_4g026080 27.059 170 118 4 307 473 1 167 1.31e-11 64.3
MS.gene004434.t1 MTR_8g469300 23.846 260 182 10 228 481 233 482 1.77e-11 67.0
MS.gene004434.t1 MTR_5g019010 25.989 177 119 6 296 466 293 463 1.83e-11 66.6
MS.gene004434.t1 MTR_8g042000 25.172 290 194 10 220 501 249 523 4.67e-11 65.5
MS.gene004434.t1 MTR_4g089030 27.749 191 126 6 276 461 291 474 5.16e-11 65.5
MS.gene004434.t1 MTR_7g016040 23.324 373 239 16 20 383 12 346 5.20e-11 64.7
MS.gene004434.t1 MTR_8g076510 20.652 460 303 20 51 489 47 465 7.99e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004434.t1 AT2G34500 68.612 497 147 2 14 510 8 495 0.0 717
MS.gene004434.t1 AT2G34490 65.121 496 167 3 14 508 8 498 0.0 671
MS.gene004434.t1 AT2G28850 60.757 502 186 4 8 507 1 493 0.0 615
MS.gene004434.t1 AT2G28860 59.363 502 193 4 8 507 1 493 0.0 610
MS.gene004434.t1 AT2G32440 24.625 467 316 15 39 495 40 480 4.92e-28 117
MS.gene004434.t1 AT2G32440 24.625 467 316 15 39 495 40 480 4.92e-28 117
MS.gene004434.t1 AT3G19270 23.684 494 316 15 17 493 8 457 1.87e-24 106
MS.gene004434.t1 AT4G19230 23.333 510 335 16 1 502 1 462 2.41e-24 106
MS.gene004434.t1 AT1G05160 24.510 510 333 18 7 500 15 488 6.88e-24 105
MS.gene004434.t1 AT1G05160 24.510 510 333 18 7 500 15 488 6.88e-24 105
MS.gene004434.t1 AT2G29090 24.364 472 305 15 34 492 40 472 1.52e-23 103
MS.gene004434.t1 AT2G29090 24.364 472 305 15 34 492 40 472 1.52e-23 103
MS.gene004434.t1 AT5G45340 23.168 505 327 16 14 502 3 462 2.14e-23 103
MS.gene004434.t1 AT4G19230 23.762 505 326 17 1 495 1 456 6.00e-23 102
MS.gene004434.t1 AT3G19270 23.667 469 299 14 17 468 8 434 1.03e-22 101
MS.gene004434.t1 AT5G45340 23.395 483 308 16 14 477 3 442 3.21e-22 99.8
MS.gene004434.t1 AT3G30180 22.541 488 325 19 17 492 10 456 7.08e-21 95.9
MS.gene004434.t1 AT2G29090 25.193 389 243 12 116 492 25 377 1.38e-19 91.3
MS.gene004434.t1 AT5G14400 25.858 437 243 16 51 461 4 385 3.18e-19 90.1
MS.gene004434.t1 AT5G14400 25.858 437 243 16 51 461 4 385 4.79e-19 90.1
MS.gene004434.t1 AT5G14400 25.926 378 209 14 100 461 9 331 2.64e-18 87.0
MS.gene004434.t1 AT1G12740 23.179 453 303 15 28 463 10 434 9.83e-18 86.3
MS.gene004434.t1 AT1G12740 23.094 459 302 15 28 463 10 440 1.21e-17 85.9
MS.gene004434.t1 AT3G28740 26.126 222 136 8 270 474 257 467 1.84e-17 85.5
MS.gene004434.t1 AT1G12740 23.991 446 287 16 27 463 38 440 2.03e-17 85.5
MS.gene004434.t1 AT1G19630 23.077 455 309 13 33 474 22 448 6.25e-17 83.6
MS.gene004434.t1 AT4G37410 29.947 187 122 6 292 474 279 460 1.50e-16 82.8
MS.gene004434.t1 AT4G37340 26.160 237 142 9 276 491 257 481 4.34e-16 81.3
MS.gene004434.t1 AT1G11680 31.548 168 107 5 295 455 273 439 5.35e-16 80.9
MS.gene004434.t1 AT5G38970 22.535 497 314 20 17 492 10 456 7.27e-16 80.5
MS.gene004434.t1 AT3G30290 25.071 351 230 12 120 463 41 365 1.98e-15 79.0
MS.gene004434.t1 AT5G36220 27.273 187 119 6 294 474 144 319 3.25e-15 77.8
MS.gene004434.t1 AT5G36220 27.273 187 119 6 294 474 290 465 6.32e-15 77.8
MS.gene004434.t1 AT5G10600 27.461 193 132 5 292 481 297 484 9.85e-15 77.0
MS.gene004434.t1 AT4G15330 30.601 183 119 5 296 473 265 444 1.09e-14 77.0
MS.gene004434.t1 AT4G15330 30.601 183 119 5 296 473 293 472 1.18e-14 77.0
MS.gene004434.t1 AT4G15350 26.738 187 123 6 296 473 291 472 1.48e-14 76.6
MS.gene004434.t1 AT4G37320 22.593 509 321 25 22 491 6 480 2.40e-14 75.9
MS.gene004434.t1 AT2G42250 27.273 187 125 4 296 473 296 480 2.76e-14 75.9
MS.gene004434.t1 AT5G57220 25.114 219 140 6 279 481 251 461 6.75e-14 74.3
MS.gene004434.t1 AT4G13770 23.093 472 288 21 21 453 11 446 7.59e-14 74.3
MS.gene004434.t1 AT1G74550 27.326 172 119 4 296 464 272 440 1.31e-13 73.6
MS.gene004434.t1 AT1G66540 25.397 189 130 5 289 474 107 287 1.37e-13 72.4
MS.gene004434.t1 AT5G38970 25.342 292 182 11 210 492 111 375 1.49e-13 72.8
MS.gene004434.t1 AT5G38970 25.342 292 182 11 210 492 111 375 1.49e-13 72.8
MS.gene004434.t1 AT5G06905 25.946 185 119 5 296 464 287 469 1.61e-13 73.6
MS.gene004434.t1 AT5G24900 25.150 167 118 3 309 472 219 381 2.25e-13 72.4
MS.gene004434.t1 AT5G35715 27.811 169 117 4 296 461 225 391 2.60e-13 72.4
MS.gene004434.t1 AT1G66540 25.500 200 132 6 289 479 166 357 2.68e-13 72.0
MS.gene004434.t1 AT3G20940 25.112 223 150 5 257 473 268 479 3.42e-13 72.4
MS.gene004434.t1 AT5G24900 25.150 167 118 3 309 472 336 498 4.31e-13 72.0
MS.gene004434.t1 AT3G14630 24.498 249 169 7 230 473 246 480 4.96e-13 71.6
MS.gene004434.t1 AT2G23220 24.180 244 155 8 236 476 258 474 5.60e-13 71.6
MS.gene004434.t1 AT3G14630 24.498 249 169 7 230 473 301 535 5.98e-13 71.6
MS.gene004434.t1 AT4G13310 30.719 153 97 5 304 451 294 442 6.35e-13 71.2
MS.gene004434.t1 AT5G38450 32.653 196 122 6 307 499 325 513 6.60e-13 71.6
MS.gene004434.t1 AT3G20935 27.841 176 119 4 304 473 131 304 8.60e-13 70.1
MS.gene004434.t1 AT1G24540 25.600 250 168 7 227 464 240 483 9.30e-13 70.9
MS.gene004434.t1 AT3G20080 29.012 162 107 3 300 455 165 324 9.68e-13 70.5
MS.gene004434.t1 AT3G20080 29.012 162 107 3 300 455 165 324 9.68e-13 70.5
MS.gene004434.t1 AT3G20080 29.012 162 107 3 300 455 165 324 9.68e-13 70.5
MS.gene004434.t1 AT3G20080 29.012 162 107 3 300 455 165 324 9.68e-13 70.5
MS.gene004434.t1 AT4G36220 29.310 174 113 5 296 464 305 473 1.44e-12 70.5
MS.gene004434.t1 AT3G20080 29.012 162 107 3 300 455 302 461 2.09e-12 69.7
MS.gene004434.t1 AT3G20080 29.012 162 107 3 300 455 302 461 2.09e-12 69.7
MS.gene004434.t1 AT4G22690 29.444 180 119 6 298 473 343 518 2.28e-12 69.7
MS.gene004434.t1 AT4G22710 29.775 178 117 6 300 473 314 487 2.40e-12 69.7
MS.gene004434.t1 AT3G20090 23.887 247 167 6 235 473 108 341 2.69e-12 68.9
MS.gene004434.t1 AT3G20090 23.887 247 167 6 235 473 246 479 2.93e-12 69.3
MS.gene004434.t1 AT2G29090 22.006 309 207 9 34 336 40 320 3.22e-12 68.6
MS.gene004434.t1 AT1G73340 20.157 511 338 17 24 508 20 486 3.28e-12 69.3
MS.gene004434.t1 AT1G64930 30.939 181 117 7 299 473 300 478 3.49e-12 69.3
MS.gene004434.t1 AT4G15300 22.388 469 308 18 14 463 4 435 4.90e-12 68.6
MS.gene004434.t1 AT1G64940 33.333 171 106 7 299 464 302 469 5.23e-12 68.6
MS.gene004434.t1 AT4G15300 23.529 357 228 13 120 463 101 425 5.74e-12 68.2
MS.gene004434.t1 AT5G10610 25.907 193 135 5 292 481 281 468 5.78e-12 68.6
MS.gene004434.t1 AT3G20950 23.333 240 165 6 241 473 255 482 5.93e-12 68.6
MS.gene004434.t1 AT1G50520 24.299 214 146 6 261 467 269 473 7.42e-12 68.2
MS.gene004434.t1 AT1G13090 25.373 201 141 6 258 456 254 447 7.42e-12 68.2
MS.gene004434.t1 AT3G20130 26.486 185 126 5 296 473 269 450 7.64e-12 68.2
MS.gene004434.t1 AT3G20130 26.486 185 126 5 296 473 297 478 7.83e-12 68.2
MS.gene004434.t1 AT4G15110 24.783 230 140 5 304 503 353 579 7.92e-12 68.2
MS.gene004434.t1 AT1G19630 22.326 430 295 13 33 449 22 425 1.12e-11 67.4
MS.gene004434.t1 AT2G23190 22.200 500 301 22 20 476 49 503 1.31e-11 67.4
MS.gene004434.t1 AT4G15396 23.433 367 236 13 111 463 101 436 1.41e-11 67.0
MS.gene004434.t1 AT3G20110 23.967 242 163 5 257 490 264 492 1.42e-11 67.4
MS.gene004434.t1 AT5G24910 25.150 167 118 3 309 472 341 503 1.44e-11 67.4
MS.gene004434.t1 AT5G25120 24.038 312 208 10 172 466 156 455 1.44e-11 67.0
MS.gene004434.t1 AT4G15396 23.433 367 236 13 111 463 101 436 1.52e-11 67.0
MS.gene004434.t1 AT5G38970 22.611 429 268 17 17 425 10 394 1.56e-11 66.6
MS.gene004434.t1 AT1G55940 24.062 453 299 22 38 472 40 465 1.86e-11 67.0
MS.gene004434.t1 AT3G48270 27.083 192 127 6 276 461 261 445 2.13e-11 66.6
MS.gene004434.t1 AT3G10570 25.824 182 122 6 289 464 298 472 2.71e-11 66.2
MS.gene004434.t1 AT5G67310 26.786 224 152 6 292 513 293 506 2.85e-11 66.2
MS.gene004434.t1 AT3G48290 29.167 192 123 6 276 461 263 447 3.05e-11 66.2
MS.gene004434.t1 AT5G04660 26.738 187 124 7 285 464 291 471 5.05e-11 65.5
MS.gene004434.t1 AT3G48300 27.976 168 112 5 300 461 279 443 6.53e-11 65.1
MS.gene004434.t1 AT3G48300 27.976 168 112 5 300 461 281 445 6.56e-11 65.1

Find 147 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTCTCATTGATTTC+TGG 0.072609 2.4:+67132749 MS.gene004434:CDS
CAAATCTCTCTCGGGCTTTC+AGG 0.177380 2.4:-67132567 None:intergenic
CGTCGACTTCGTCGCTTCTT+TGG 0.185585 2.4:+67132893 MS.gene004434:CDS
GAAGCTTCCGTTTGACTTTC+CGG 0.201330 2.4:+67132624 MS.gene004434:CDS
ACTTTCTCGTGATATGAATT+TGG 0.222895 2.4:+67132507 MS.gene004434:CDS
AAAAGAAACTTTCTAGCATT+TGG 0.244128 2.4:+67133258 MS.gene004434:CDS
TTCGTGACCCAACAAAATTT+TGG 0.247019 2.4:+67132131 MS.gene004434:CDS
TCTTTGGGCGGTGGCATTGC+TGG 0.247238 2.4:+67132909 MS.gene004434:CDS
CATCTTGTGGGCCATCCTTT+TGG 0.257964 2.4:+67132283 MS.gene004434:CDS
ATACTAGGTATGGGATTAAT+TGG 0.259565 2.4:-67131997 None:intergenic
ACAAGGTTTCCTTCATTTGC+TGG 0.277953 2.4:+67133462 MS.gene004434:CDS
AAGCGCCTTAGGAGTGAAAT+TGG 0.279019 2.4:-67132383 None:intergenic
CTTTCTTCTGAAAACCGGTC+TGG 0.291774 2.4:-67133216 None:intergenic
GTCGACTTCGTCGCTTCTTT+GGG 0.319856 2.4:+67132894 MS.gene004434:CDS
AAGCTTCCGTTTGACTTTCC+GGG 0.333180 2.4:+67132625 MS.gene004434:CDS
CTCACTCTGGCGAGAACCTC+TGG 0.336155 2.4:-67132940 None:intergenic
CGACTCCGGCGTCCAGATTC+CGG 0.337286 2.4:-67132974 None:intergenic
GGGAAGAAGTTGCCGGAATC+TGG 0.339302 2.4:+67132962 MS.gene004434:CDS
TCCTTCATTTGCTGGGGTTG+TGG 0.348629 2.4:+67133470 MS.gene004434:CDS
AGGGAAAACTATTGCACCTT+TGG 0.356566 2.4:-67133142 None:intergenic
CAAGGTTTCCTTCATTTGCT+GGG 0.359215 2.4:+67133463 MS.gene004434:CDS
TCCTCAAACATCTTAAATCA+TGG 0.362523 2.4:+67132437 MS.gene004434:CDS
GAGTCGGTCAACGGCGAGTC+TGG 0.362607 2.4:-67132665 None:intergenic
TGTACAACCCGCTAGGGTTC+CGG 0.366222 2.4:-67132689 None:intergenic
TCTTTAGTCCTCCCATTCAT+AGG 0.366396 2.4:+67132091 MS.gene004434:CDS
AAAACCCTACAATCATCTTT+AGG 0.377601 2.4:-67133423 None:intergenic
CTTGTCTTTCTTCTGAAAAC+CGG 0.383703 2.4:-67133221 None:intergenic
GGCAGAGCCGTTTCCGTTGA+CGG 0.391585 2.4:+67133104 MS.gene004434:CDS
GGGAAAACTATTGCACCTTT+GGG 0.392533 2.4:-67133141 None:intergenic
TGATAACATCGGAGCAGTTA+AGG 0.396633 2.4:+67133004 MS.gene004434:CDS
TTTGTCCTAAAGATGATTGT+AGG 0.396653 2.4:+67133418 MS.gene004434:CDS
TTTCTTCTGAAAACCGGTCT+GGG 0.399083 2.4:-67133215 None:intergenic
CGGGCTTTCAGGCCCAAATA+AGG 0.400260 2.4:-67132556 None:intergenic
TTCTCCACCAACGCTGAAAT+CGG 0.405544 2.4:+67132832 MS.gene004434:CDS
AGGATAAAGCATACTAGGTA+TGG 0.406804 2.4:-67132007 None:intergenic
AACTTTCTAGCATTTGGAGC+TGG 0.407002 2.4:+67133264 MS.gene004434:CDS
GCATTACGGAACGCAGTTCC+CGG 0.407646 2.4:-67132643 None:intergenic
CCAAAGAGTTTCTTACCAAA+AGG 0.418748 2.4:-67132298 None:intergenic
CCTCTGGCCCACACATTGGT+GGG 0.422184 2.4:-67133289 None:intergenic
TCTCATTGATTTCTGGATGC+AGG 0.429468 2.4:+67132756 MS.gene004434:CDS
CAACGGCGAGTCTGGCATTA+CGG 0.443747 2.4:-67132657 None:intergenic
TAAGATTCCGTCAACGGAAA+CGG 0.445106 2.4:-67133111 None:intergenic
GAGAAAGAGGATAATAATAG+AGG 0.454708 2.4:-67131968 None:intergenic
TAGACTCATGGGCTTGAGCT+TGG 0.460002 2.4:-67132471 None:intergenic
TACTTTGGTGCCGCATGTTG+CGG 0.460799 2.4:+67133083 MS.gene004434:CDS
TTACCGACCACCAGCTACTT+TGG 0.462145 2.4:+67133068 MS.gene004434:CDS
ACCTCTGGCCCACACATTGG+TGG 0.463771 2.4:-67133290 None:intergenic
GCCCAACGCTTTCCATCTTG+TGG 0.466664 2.4:+67132270 MS.gene004434:CDS
CCCATGATTTAAGATGTTTG+AGG 0.466832 2.4:-67132438 None:intergenic
AATCATGTTCAAATCTCTCT+CGG 0.472656 2.4:-67132576 None:intergenic
GATAACATCGGAGCAGTTAA+GGG 0.472782 2.4:+67133005 MS.gene004434:CDS
ACTTTCTAGCATTTGGAGCT+GGG 0.473086 2.4:+67133265 MS.gene004434:CDS
AGCGGCGAGTAAGGAGAAGA+TGG 0.473213 2.4:+67132711 MS.gene004434:CDS
AGACTCATGGGCTTGAGCTT+GGG 0.475253 2.4:-67132470 None:intergenic
GAGATGGCGTTTCCTATGAA+TGG 0.478084 2.4:-67132103 None:intergenic
GGGAGGACTAAAGATGGGCC+TGG 0.482100 2.4:-67132082 None:intergenic
AAAACCGGTCTGGGTCGAAC+CGG 0.483391 2.4:-67133206 None:intergenic
GTGTATGTAGAAAGCGCCTT+AGG 0.484393 2.4:-67132394 None:intergenic
GGGTTGTACAGCGGCGAGTA+AGG 0.485139 2.4:+67132702 MS.gene004434:CDS
CGCACCCAATTTCACTCCTA+AGG 0.490336 2.4:+67132378 MS.gene004434:CDS
CAAAAGGATGGCCCACAAGA+TGG 0.491389 2.4:-67132282 None:intergenic
CCCAACGCTTTCCATCTTGT+GGG 0.493749 2.4:+67132271 MS.gene004434:CDS
GGAAAAGGATAAAGCATACT+AGG 0.495262 2.4:-67132012 None:intergenic
GATTACTTTCTCTTCAACGT+TGG 0.495270 2.4:+67132595 MS.gene004434:CDS
CCCACAAGATGGAAAGCGTT+GGG 0.496495 2.4:-67132271 None:intergenic
CCTCAAACATCTTAAATCAT+GGG 0.497070 2.4:+67132438 MS.gene004434:CDS
CGGTCTGGGTCGAACCGGTC+CGG 0.505094 2.4:-67133201 None:intergenic
TAATTGGTTGAAAGAGAAAG+AGG 0.508507 2.4:-67131981 None:intergenic
GATGATTCAAAGCGTACCTC+TGG 0.510126 2.4:-67133305 None:intergenic
GGCAACTTCTTCCCTCACTC+TGG 0.510937 2.4:-67132953 None:intergenic
ACATGCGGCACCAAAGTAGC+TGG 0.511652 2.4:-67133078 None:intergenic
TTGTCCTAAAGATGATTGTA+GGG 0.514709 2.4:+67133419 MS.gene004434:CDS
ACACAATCTTATCTACATGA+TGG 0.515797 2.4:+67132324 MS.gene004434:CDS
AGTAAGGAGAAGATGGAGAA+AGG 0.515835 2.4:+67132718 MS.gene004434:CDS
AAGATGACTCGAACGCCGAA+GGG 0.516284 2.4:-67133161 None:intergenic
GCAGTTCCCGGAAAGTCAAA+CGG 0.517756 2.4:-67132631 None:intergenic
AGATGGCGTTTCCTATGAAT+GGG 0.519794 2.4:-67132102 None:intergenic
ACCACCGATTTCAGCGTTGG+TGG 0.520559 2.4:-67132836 None:intergenic
ATGAATGGGAGGACTAAAGA+TGG 0.523224 2.4:-67132088 None:intergenic
TTCGTCGCTTCTTTGGGCGG+TGG 0.523611 2.4:+67132900 MS.gene004434:CDS
AGCTGGGCCCCACCAATGTG+TGG 0.524040 2.4:+67133281 MS.gene004434:CDS
GTTCAATGAGGAGAAGGAAA+AGG 0.524798 2.4:-67132027 None:intergenic
GAAAGCGTTGGGCTTTACAT+TGG 0.526946 2.4:-67132260 None:intergenic
GACTAAAGATGGGCCTGGGA+TGG 0.528214 2.4:-67132077 None:intergenic
TGAAATTGGGTGCGATTCGA+CGG 0.528462 2.4:-67132369 None:intergenic
TCCACAACCCCAGCAAATGA+AGG 0.530533 2.4:-67133471 None:intergenic
CCGCTAGGGTTCCGGCGAGT+CGG 0.533184 2.4:-67132681 None:intergenic
CTCTGGCCCACACATTGGTG+GGG 0.534062 2.4:-67133288 None:intergenic
GACTTCGTCGCTTCTTTGGG+CGG 0.534699 2.4:+67132897 MS.gene004434:CDS
AAAGATGACTCGAACGCCGA+AGG 0.534701 2.4:-67133162 None:intergenic
CACAATCTTATCTACATGAT+GGG 0.539508 2.4:+67132325 MS.gene004434:CDS
ATAACCACCGATTTCAGCGT+TGG 0.540093 2.4:-67132839 None:intergenic
TCACGAGAAAGTACACGGAT+TGG 0.540148 2.4:-67132496 None:intergenic
CAGAAATCAATGAGACAAGA+AGG 0.540242 2.4:-67132748 None:intergenic
AGATCTGTTCAATGAGGAGA+AGG 0.541911 2.4:-67132033 None:intergenic
TCGCCGCTGTACAACCCGCT+AGG 0.542956 2.4:-67132696 None:intergenic
GGAGGACTAAAGATGGGCCT+GGG 0.543474 2.4:-67132081 None:intergenic
ATCATGTTCAAATCTCTCTC+GGG 0.544006 2.4:-67132575 None:intergenic
AACGGCTCTGCCGCAACATG+CGG 0.544241 2.4:-67133093 None:intergenic
CGACTCGCCGGAACCCTAGC+GGG 0.546189 2.4:+67132682 MS.gene004434:CDS
CCCACCAATGTGTGGGCCAG+AGG 0.549703 2.4:+67133289 MS.gene004434:CDS
CACGGATTGGAATAGACTCA+TGG 0.553184 2.4:-67132483 None:intergenic
AGCGCCTTAGGAGTGAAATT+GGG 0.553635 2.4:-67132382 None:intergenic
CGCCGCTGTACAACCCGCTA+GGG 0.554607 2.4:-67132695 None:intergenic
ATTTCAGCGTTGGTGGAGAA+CGG 0.556836 2.4:-67132829 None:intergenic
GCCCACAAGATGGAAAGCGT+TGG 0.558548 2.4:-67132272 None:intergenic
GGATAAAGCATACTAGGTAT+GGG 0.559100 2.4:-67132006 None:intergenic
AGAGGTTCTCGCCAGAGTGA+GGG 0.564227 2.4:+67132942 MS.gene004434:CDS
CGTACCTCTGGCCCACACAT+TGG 0.569147 2.4:-67133293 None:intergenic
ATCGTGTAAGATTCCGTCAA+CGG 0.575389 2.4:-67133117 None:intergenic
AAGGTTTCCTTCATTTGCTG+GGG 0.575823 2.4:+67133464 MS.gene004434:CDS
ACGCGTCTTGTGCTGCGAAG+AGG 0.576235 2.4:-67132873 None:intergenic
GGTTCCGGCGAGTCGGTCAA+CGG 0.576734 2.4:-67132674 None:intergenic
AGAGTTTCTTACCAAAAGGA+TGG 0.576923 2.4:-67132294 None:intergenic
CACACAAGCGGTGGCGCGTG+AGG 0.579947 2.4:+67133038 MS.gene004434:CDS
AGAGTGAGGGAAGAAGTTGC+CGG 0.580981 2.4:+67132955 MS.gene004434:CDS
CTCGCCGTTGACCGACTCGC+CGG 0.582101 2.4:+67132670 MS.gene004434:CDS
TGATTTCAAGAGAGATAGAA+CGG 0.585491 2.4:+67133362 MS.gene004434:CDS
CAGAGGTTCTCGCCAGAGTG+AGG 0.585749 2.4:+67132941 MS.gene004434:CDS
GCTGGGCCCCACCAATGTGT+GGG 0.587228 2.4:+67133282 MS.gene004434:CDS
TTCAAGAGAGATAGAACGGA+CGG 0.593977 2.4:+67133366 MS.gene004434:CDS
TTCTGTCTAAGCGTTGCACA+AGG 0.594634 2.4:+67133445 MS.gene004434:CDS
CGGACCGGTTCGACCCAGAC+CGG 0.595668 2.4:+67133202 MS.gene004434:CDS
TCCACCTTATCAAAATCCAC+TGG 0.602010 2.4:+67132163 MS.gene004434:CDS
TGAATGGGAGGACTAAAGAT+GGG 0.603982 2.4:-67132087 None:intergenic
ATTGCTGGATTCGCATCCAG+AGG 0.607890 2.4:+67132924 MS.gene004434:CDS
ATTGAAGAATCGAAGCTGAA+CGG 0.619411 2.4:+67132793 MS.gene004434:CDS
AACCCTAGCGGGTTGTACAG+CGG 0.621201 2.4:+67132693 MS.gene004434:CDS
ACGGATTGGAATAGACTCAT+GGG 0.622968 2.4:-67132482 None:intergenic
GTTATCAGCGTATTCGACTC+CGG 0.623325 2.4:-67132988 None:intergenic
GCACCAAAGTAGCTGGTGGT+CGG 0.639250 2.4:-67133071 None:intergenic
CCGACTCGCCGGAACCCTAG+CGG 0.646571 2.4:+67132681 MS.gene004434:CDS
TCATATCACGAGAAAGTACA+CGG 0.651010 2.4:-67132501 None:intergenic
GAATCTTACACGATTCCCAA+AGG 0.656637 2.4:+67133126 MS.gene004434:CDS
TGGGTCACGAACTAGTGAGA+TGG 0.657031 2.4:-67132119 None:intergenic
TGGCGTTTCCTATGAATGGG+AGG 0.658875 2.4:-67132099 None:intergenic
GTAGTTAGCTGAAAAGCCAG+TGG 0.671255 2.4:-67132179 None:intergenic
GTCGAATACGCTGATAACAT+CGG 0.671799 2.4:+67132993 MS.gene004434:CDS
TCCACCAACGCTGAAATCGG+TGG 0.677015 2.4:+67132835 MS.gene004434:CDS
TGCGGCACCAAAGTAGCTGG+TGG 0.679995 2.4:-67133075 None:intergenic
TCAGCGTTGGTGGAGAACGG+TGG 0.681466 2.4:-67132826 None:intergenic
AGTTGCCGGAATCTGGACGC+CGG 0.682404 2.4:+67132969 MS.gene004434:CDS
GGTATGAGATCTGTTCAATG+AGG 0.685258 2.4:-67132039 None:intergenic
TCATCTTTAGGACAAATCGT+AGG 0.690195 2.4:-67133411 None:intergenic
AATTGGGTGCGATTCGACGG+CGG 0.691141 2.4:-67132366 None:intergenic
GGAGATGAAGTACACACAAG+CGG 0.691579 2.4:+67133026 MS.gene004434:CDS
GATGAAGTACACACAAGCGG+TGG 0.696798 2.4:+67133029 MS.gene004434:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAACTTTCTAGCATT+TGG + chr2.4:67133258-67133277 MS.gene004434:CDS 25.0%
AAAACCCTACAATCATCTTT+AGG - chr2.4:67133426-67133445 None:intergenic 30.0%
AATCATGTTCAAATCTCTCT+CGG - chr2.4:67132579-67132598 None:intergenic 30.0%
ACACAATCTTATCTACATGA+TGG + chr2.4:67132324-67132343 MS.gene004434:CDS 30.0%
ACTTTCTCGTGATATGAATT+TGG + chr2.4:67132507-67132526 MS.gene004434:CDS 30.0%
ATACTAGGTATGGGATTAAT+TGG - chr2.4:67132000-67132019 None:intergenic 30.0%
CACAATCTTATCTACATGAT+GGG + chr2.4:67132325-67132344 MS.gene004434:CDS 30.0%
CCTCAAACATCTTAAATCAT+GGG + chr2.4:67132438-67132457 MS.gene004434:CDS 30.0%
GAGAAAGAGGATAATAATAG+AGG - chr2.4:67131971-67131990 None:intergenic 30.0%
TAATTGGTTGAAAGAGAAAG+AGG - chr2.4:67131984-67132003 None:intergenic 30.0%
TCCTCAAACATCTTAAATCA+TGG + chr2.4:67132437-67132456 MS.gene004434:CDS 30.0%
TGATTTCAAGAGAGATAGAA+CGG + chr2.4:67133362-67133381 MS.gene004434:CDS 30.0%
TTGTCCTAAAGATGATTGTA+GGG + chr2.4:67133419-67133438 MS.gene004434:CDS 30.0%
TTTGTCCTAAAGATGATTGT+AGG + chr2.4:67133418-67133437 MS.gene004434:CDS 30.0%
! GAACTTCCCAAAATTTTGTT+GGG - chr2.4:67132141-67132160 None:intergenic 30.0%
! TGAACTTCCCAAAATTTTGT+TGG - chr2.4:67132142-67132161 None:intergenic 30.0%
! TTTTCAGCTAACTACATCAT+TGG + chr2.4:67132187-67132206 MS.gene004434:CDS 30.0%
AGAGTTTCTTACCAAAAGGA+TGG - chr2.4:67132297-67132316 None:intergenic 35.0%
AGGATAAAGCATACTAGGTA+TGG - chr2.4:67132010-67132029 None:intergenic 35.0%
ATCATGTTCAAATCTCTCTC+GGG - chr2.4:67132578-67132597 None:intergenic 35.0%
ATTGAAGAATCGAAGCTGAA+CGG + chr2.4:67132793-67132812 MS.gene004434:CDS 35.0%
CAGAAATCAATGAGACAAGA+AGG - chr2.4:67132751-67132770 None:intergenic 35.0%
CCAAAGAGTTTCTTACCAAA+AGG - chr2.4:67132301-67132320 None:intergenic 35.0%
GATTACTTTCTCTTCAACGT+TGG + chr2.4:67132595-67132614 MS.gene004434:CDS 35.0%
GGAAAAGGATAAAGCATACT+AGG - chr2.4:67132015-67132034 None:intergenic 35.0%
GGATAAAGCATACTAGGTAT+GGG - chr2.4:67132009-67132028 None:intergenic 35.0%
! CCCATGATTTAAGATGTTTG+AGG - chr2.4:67132441-67132460 None:intergenic 35.0%
! CCTTTTGGTAAGAAACTCTT+TGG + chr2.4:67132298-67132317 MS.gene004434:CDS 35.0%
! CTTGTCTTTCTTCTGAAAAC+CGG - chr2.4:67133224-67133243 None:intergenic 35.0%
! GTTTTCAGAAGAAAGACAAG+AGG + chr2.4:67133224-67133243 MS.gene004434:CDS 35.0%
! TCATATCACGAGAAAGTACA+CGG - chr2.4:67132504-67132523 None:intergenic 35.0%
! TCGTGACCCAACAAAATTTT+GGG + chr2.4:67132132-67132151 MS.gene004434:CDS 35.0%
! TTCGTGACCCAACAAAATTT+TGG + chr2.4:67132131-67132150 MS.gene004434:CDS 35.0%
!! AAAGCCAGTGGATTTTGATA+AGG - chr2.4:67132170-67132189 None:intergenic 35.0%
!! CTTCTTGTCTCATTGATTTC+TGG + chr2.4:67132749-67132768 MS.gene004434:CDS 35.0%
!! TCATCTTTAGGACAAATCGT+AGG - chr2.4:67133414-67133433 None:intergenic 35.0%
!! TTTTCAGGGTTTTACTGAAC+CGG + chr2.4:67133182-67133201 MS.gene004434:CDS 35.0%
AACAAAAAACGTTCCATCCC+AGG + chr2.4:67132064-67132083 MS.gene004434:CDS 40.0%
ACAAGGTTTCCTTCATTTGC+TGG + chr2.4:67133462-67133481 MS.gene004434:CDS 40.0%
AGATCTGTTCAATGAGGAGA+AGG - chr2.4:67132036-67132055 None:intergenic 40.0%
AGATGGCGTTTCCTATGAAT+GGG - chr2.4:67132105-67132124 None:intergenic 40.0%
AGGGAAAACTATTGCACCTT+TGG - chr2.4:67133145-67133164 None:intergenic 40.0%
AGTAAGGAGAAGATGGAGAA+AGG + chr2.4:67132718-67132737 MS.gene004434:CDS 40.0%
ATCGTGTAAGATTCCGTCAA+CGG - chr2.4:67133120-67133139 None:intergenic 40.0%
ATGAATGGGAGGACTAAAGA+TGG - chr2.4:67132091-67132110 None:intergenic 40.0%
CAAGGTTTCCTTCATTTGCT+GGG + chr2.4:67133463-67133482 MS.gene004434:CDS 40.0%
GAATCTTACACGATTCCCAA+AGG + chr2.4:67133126-67133145 MS.gene004434:CDS 40.0%
GATAACATCGGAGCAGTTAA+GGG + chr2.4:67133005-67133024 MS.gene004434:CDS 40.0%
GGGAAAACTATTGCACCTTT+GGG - chr2.4:67133144-67133163 None:intergenic 40.0%
GGTATGAGATCTGTTCAATG+AGG - chr2.4:67132042-67132061 None:intergenic 40.0%
GTCGAATACGCTGATAACAT+CGG + chr2.4:67132993-67133012 MS.gene004434:CDS 40.0%
GTTCAATGAGGAGAAGGAAA+AGG - chr2.4:67132030-67132049 None:intergenic 40.0%
TAAGATTCCGTCAACGGAAA+CGG - chr2.4:67133114-67133133 None:intergenic 40.0%
TCCACCTTATCAAAATCCAC+TGG + chr2.4:67132163-67132182 MS.gene004434:CDS 40.0%
TCTTTAGTCCTCCCATTCAT+AGG + chr2.4:67132091-67132110 MS.gene004434:CDS 40.0%
TGAATGGGAGGACTAAAGAT+GGG - chr2.4:67132090-67132109 None:intergenic 40.0%
TGATAACATCGGAGCAGTTA+AGG + chr2.4:67133004-67133023 MS.gene004434:CDS 40.0%
TTCAAGAGAGATAGAACGGA+CGG + chr2.4:67133366-67133385 MS.gene004434:CDS 40.0%
! AACGTCTCAGAAAGTTTTCG+TGG + chr2.4:67132531-67132550 MS.gene004434:CDS 40.0%
! AACTTTCTAGCATTTGGAGC+TGG + chr2.4:67133264-67133283 MS.gene004434:CDS 40.0%
! AAGGTTTCCTTCATTTGCTG+GGG + chr2.4:67133464-67133483 MS.gene004434:CDS 40.0%
! ACGTCTCAGAAAGTTTTCGT+GGG + chr2.4:67132532-67132551 MS.gene004434:CDS 40.0%
! ACTTTCTAGCATTTGGAGCT+GGG + chr2.4:67133265-67133284 MS.gene004434:CDS 40.0%
! AGGTGCAATAGTTTTCCCTT+CGG + chr2.4:67133146-67133165 MS.gene004434:CDS 40.0%
! AGTTTTCGTGGGACCTTATT+TGG + chr2.4:67132543-67132562 MS.gene004434:CDS 40.0%
! TTTCTTCTGAAAACCGGTCT+GGG - chr2.4:67133218-67133237 None:intergenic 40.0%
!! ACGGATTGGAATAGACTCAT+GGG - chr2.4:67132485-67132504 None:intergenic 40.0%
!! GTTTTCGTGGGACCTTATTT+GGG + chr2.4:67132544-67132563 MS.gene004434:CDS 40.0%
!! TCTCATTGATTTCTGGATGC+AGG + chr2.4:67132756-67132775 MS.gene004434:CDS 40.0%
!!! GGATGGAACGTTTTTTGTTG+AGG - chr2.4:67132063-67132082 None:intergenic 40.0%
AAGCGCCTTAGGAGTGAAAT+TGG - chr2.4:67132386-67132405 None:intergenic 45.0%
AGCGCCTTAGGAGTGAAATT+GGG - chr2.4:67132385-67132404 None:intergenic 45.0%
GAGATGGCGTTTCCTATGAA+TGG - chr2.4:67132106-67132125 None:intergenic 45.0%
GATGATTCAAAGCGTACCTC+TGG - chr2.4:67133308-67133327 None:intergenic 45.0%
GGAGATGAAGTACACACAAG+CGG + chr2.4:67133026-67133045 MS.gene004434:CDS 45.0%
GTAGTTAGCTGAAAAGCCAG+TGG - chr2.4:67132182-67132201 None:intergenic 45.0%
GTTATCAGCGTATTCGACTC+CGG - chr2.4:67132991-67133010 None:intergenic 45.0%
TGAAATTGGGTGCGATTCGA+CGG - chr2.4:67132372-67132391 None:intergenic 45.0%
TTCTCCACCAACGCTGAAAT+CGG + chr2.4:67132832-67132851 MS.gene004434:CDS 45.0%
! AAGCTTCCGTTTGACTTTCC+GGG + chr2.4:67132625-67132644 MS.gene004434:CDS 45.0%
! CTTTCTTCTGAAAACCGGTC+TGG - chr2.4:67133219-67133238 None:intergenic 45.0%
! GAAGCTTCCGTTTGACTTTC+CGG + chr2.4:67132624-67132643 MS.gene004434:CDS 45.0%
! GCGTTCGAGTCATCTTTTCA+GGG + chr2.4:67133168-67133187 MS.gene004434:CDS 45.0%
! TCACGAGAAAGTACACGGAT+TGG - chr2.4:67132499-67132518 None:intergenic 45.0%
! TTCTGTCTAAGCGTTGCACA+AGG + chr2.4:67133445-67133464 MS.gene004434:CDS 45.0%
!! ATAACCACCGATTTCAGCGT+TGG - chr2.4:67132842-67132861 None:intergenic 45.0%
!! ATTTCAGCGTTGGTGGAGAA+CGG - chr2.4:67132832-67132851 None:intergenic 45.0%
!! CACGGATTGGAATAGACTCA+TGG - chr2.4:67132486-67132505 None:intergenic 45.0%
!! GAAAGCGTTGGGCTTTACAT+TGG - chr2.4:67132263-67132282 None:intergenic 45.0%
!! GCCAGTGGATTTTGATAAGG+TGG - chr2.4:67132167-67132186 None:intergenic 45.0%
!! GTGTATGTAGAAAGCGCCTT+AGG - chr2.4:67132397-67132416 None:intergenic 45.0%
AAAGATGACTCGAACGCCGA+AGG - chr2.4:67133165-67133184 None:intergenic 50.0%
AAGATGACTCGAACGCCGAA+GGG - chr2.4:67133164-67133183 None:intergenic 50.0%
AGACTCATGGGCTTGAGCTT+GGG - chr2.4:67132473-67132492 None:intergenic 50.0%
AGAGTGAGGGAAGAAGTTGC+CGG + chr2.4:67132955-67132974 MS.gene004434:CDS 50.0%
ATTGCTGGATTCGCATCCAG+AGG + chr2.4:67132924-67132943 MS.gene004434:CDS 50.0%
CAAAAGGATGGCCCACAAGA+TGG - chr2.4:67132285-67132304 None:intergenic 50.0%
CAAATCTCTCTCGGGCTTTC+AGG - chr2.4:67132570-67132589 None:intergenic 50.0%
CCCAACGCTTTCCATCTTGT+GGG + chr2.4:67132271-67132290 MS.gene004434:CDS 50.0%
CGCACCCAATTTCACTCCTA+AGG + chr2.4:67132378-67132397 MS.gene004434:CDS 50.0%
GATGAAGTACACACAAGCGG+TGG + chr2.4:67133029-67133048 MS.gene004434:CDS 50.0%
GCAGTTCCCGGAAAGTCAAA+CGG - chr2.4:67132634-67132653 None:intergenic 50.0%
TAGACTCATGGGCTTGAGCT+TGG - chr2.4:67132474-67132493 None:intergenic 50.0%
TCCACAACCCCAGCAAATGA+AGG - chr2.4:67133474-67133493 None:intergenic 50.0%
TGGCGTTTCCTATGAATGGG+AGG - chr2.4:67132102-67132121 None:intergenic 50.0%
TGGGTCACGAACTAGTGAGA+TGG - chr2.4:67132122-67132141 None:intergenic 50.0%
TTACCGACCACCAGCTACTT+TGG + chr2.4:67133068-67133087 MS.gene004434:CDS 50.0%
! AGGGTTTTACTGAACCGGAC+CGG + chr2.4:67133187-67133206 MS.gene004434:CDS 50.0%
! CATCTTGTGGGCCATCCTTT+TGG + chr2.4:67132283-67132302 MS.gene004434:CDS 50.0%
! GGCGTTCGAGTCATCTTTTC+AGG + chr2.4:67133167-67133186 MS.gene004434:CDS 50.0%
! GTCGACTTCGTCGCTTCTTT+GGG + chr2.4:67132894-67132913 MS.gene004434:CDS 50.0%
! TCCTTCATTTGCTGGGGTTG+TGG + chr2.4:67133470-67133489 MS.gene004434:CDS 50.0%
!! CCCACAAGATGGAAAGCGTT+GGG - chr2.4:67132274-67132293 None:intergenic 50.0%
!! TACTTTGGTGCCGCATGTTG+CGG + chr2.4:67133083-67133102 MS.gene004434:CDS 50.0%
AAAACCGGTCTGGGTCGAAC+CGG - chr2.4:67133209-67133228 None:intergenic 55.0%
AATTGGGTGCGATTCGACGG+CGG - chr2.4:67132369-67132388 None:intergenic 55.0%
ACATGCGGCACCAAAGTAGC+TGG - chr2.4:67133081-67133100 None:intergenic 55.0%
AGAGGTTCTCGCCAGAGTGA+GGG + chr2.4:67132942-67132961 MS.gene004434:CDS 55.0%
AGCGGCGAGTAAGGAGAAGA+TGG + chr2.4:67132711-67132730 MS.gene004434:CDS 55.0%
CAACGGCGAGTCTGGCATTA+CGG - chr2.4:67132660-67132679 None:intergenic 55.0%
CGGGCTTTCAGGCCCAAATA+AGG - chr2.4:67132559-67132578 None:intergenic 55.0%
GACTAAAGATGGGCCTGGGA+TGG - chr2.4:67132080-67132099 None:intergenic 55.0%
GACTTCGTCGCTTCTTTGGG+CGG + chr2.4:67132897-67132916 MS.gene004434:CDS 55.0%
GCACCAAAGTAGCTGGTGGT+CGG - chr2.4:67133074-67133093 None:intergenic 55.0%
GCATTACGGAACGCAGTTCC+CGG - chr2.4:67132646-67132665 None:intergenic 55.0%
GCCCAACGCTTTCCATCTTG+TGG + chr2.4:67132270-67132289 MS.gene004434:CDS 55.0%
GGAGGACTAAAGATGGGCCT+GGG - chr2.4:67132084-67132103 None:intergenic 55.0%
GGCAACTTCTTCCCTCACTC+TGG - chr2.4:67132956-67132975 None:intergenic 55.0%
GGGAAGAAGTTGCCGGAATC+TGG + chr2.4:67132962-67132981 MS.gene004434:CDS 55.0%
TCCACCAACGCTGAAATCGG+TGG + chr2.4:67132835-67132854 MS.gene004434:CDS 55.0%
TGTACAACCCGCTAGGGTTC+CGG - chr2.4:67132692-67132711 None:intergenic 55.0%
! GCCCACAAGATGGAAAGCGT+TGG - chr2.4:67132275-67132294 None:intergenic 55.0%
!! AACCCTAGCGGGTTGTACAG+CGG + chr2.4:67132693-67132712 MS.gene004434:CDS 55.0%
!! ACCACCGATTTCAGCGTTGG+TGG - chr2.4:67132839-67132858 None:intergenic 55.0%
!! CGTCGACTTCGTCGCTTCTT+TGG + chr2.4:67132893-67132912 MS.gene004434:CDS 55.0%
AACGGCTCTGCCGCAACATG+CGG - chr2.4:67133096-67133115 None:intergenic 60.0%
ACCTCTGGCCCACACATTGG+TGG - chr2.4:67133293-67133312 None:intergenic 60.0%
ACGCGTCTTGTGCTGCGAAG+AGG - chr2.4:67132876-67132895 None:intergenic 60.0%
AGTTGCCGGAATCTGGACGC+CGG + chr2.4:67132969-67132988 MS.gene004434:CDS 60.0%
CAGAGGTTCTCGCCAGAGTG+AGG + chr2.4:67132941-67132960 MS.gene004434:CDS 60.0%
CCTCTGGCCCACACATTGGT+GGG - chr2.4:67133292-67133311 None:intergenic 60.0%
CGTACCTCTGGCCCACACAT+TGG - chr2.4:67133296-67133315 None:intergenic 60.0%
CTCACTCTGGCGAGAACCTC+TGG - chr2.4:67132943-67132962 None:intergenic 60.0%
CTCTGGCCCACACATTGGTG+GGG - chr2.4:67133291-67133310 None:intergenic 60.0%
GGCAGAGCCGTTTCCGTTGA+CGG + chr2.4:67133104-67133123 MS.gene004434:CDS 60.0%
GGGAGGACTAAAGATGGGCC+TGG - chr2.4:67132085-67132104 None:intergenic 60.0%
GGGTTGTACAGCGGCGAGTA+AGG + chr2.4:67132702-67132721 MS.gene004434:CDS 60.0%
TCTTTGGGCGGTGGCATTGC+TGG + chr2.4:67132909-67132928 MS.gene004434:CDS 60.0%
TGCGGCACCAAAGTAGCTGG+TGG - chr2.4:67133078-67133097 None:intergenic 60.0%
TTCGTCGCTTCTTTGGGCGG+TGG + chr2.4:67132900-67132919 MS.gene004434:CDS 60.0%
!! TCAGCGTTGGTGGAGAACGG+TGG - chr2.4:67132829-67132848 None:intergenic 60.0%
AGCTGGGCCCCACCAATGTG+TGG + chr2.4:67133281-67133300 MS.gene004434:CDS 65.0%
CCCACCAATGTGTGGGCCAG+AGG + chr2.4:67133289-67133308 MS.gene004434:CDS 65.0%
CGACTCCGGCGTCCAGATTC+CGG - chr2.4:67132977-67132996 None:intergenic 65.0%
CGCCGCTGTACAACCCGCTA+GGG - chr2.4:67132698-67132717 None:intergenic 65.0%
GAGTCGGTCAACGGCGAGTC+TGG - chr2.4:67132668-67132687 None:intergenic 65.0%
GCTGGGCCCCACCAATGTGT+GGG + chr2.4:67133282-67133301 MS.gene004434:CDS 65.0%
GGTTCCGGCGAGTCGGTCAA+CGG - chr2.4:67132677-67132696 None:intergenic 65.0%
TCGCCGCTGTACAACCCGCT+AGG - chr2.4:67132699-67132718 None:intergenic 65.0%
CACACAAGCGGTGGCGCGTG+AGG + chr2.4:67133038-67133057 MS.gene004434:CDS 70.0%
CCGCTAGGGTTCCGGCGAGT+CGG - chr2.4:67132684-67132703 None:intergenic 70.0%
CGACTCGCCGGAACCCTAGC+GGG + chr2.4:67132682-67132701 MS.gene004434:CDS 70.0%
CGGTCTGGGTCGAACCGGTC+CGG - chr2.4:67133204-67133223 None:intergenic 70.0%
! CCGACTCGCCGGAACCCTAG+CGG + chr2.4:67132681-67132700 MS.gene004434:CDS 70.0%
!! CGGACCGGTTCGACCCAGAC+CGG + chr2.4:67133202-67133221 MS.gene004434:CDS 70.0%
!! CTCGCCGTTGACCGACTCGC+CGG + chr2.4:67132670-67132689 MS.gene004434:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 67131953 67133506 67131953 ID=MS.gene004434
chr2.4 mRNA 67131953 67133506 67131953 ID=MS.gene004434.t1;Parent=MS.gene004434
chr2.4 exon 67131953 67133506 67131953 ID=MS.gene004434.t1.exon1;Parent=MS.gene004434.t1
chr2.4 CDS 67131953 67133506 67131953 ID=cds.MS.gene004434.t1;Parent=MS.gene004434.t1
Gene Sequence

>MS.gene004434

ATGAATATGAATGCTCCTCTATTATTATCCTCTTTCTCTTTCAACCAATTAATCCCATACCTAGTATGCTTTATCCTTTTCCTTCTCCTCATTGAACAGATCTCATACCTCAACAAAAAACGTTCCATCCCAGGCCCATCTTTAGTCCTCCCATTCATAGGAAACGCCATCTCACTAGTTCGTGACCCAACAAAATTTTGGGAAGTTCAATCCACCTTATCAAAATCCACTGGCTTTTCAGCTAACTACATCATTGGACTCTTTATAGTCTTCATTAGAGACACTGAACTCTCTCATAAAGTTTTCTCCAATGTAAAGCCCAACGCTTTCCATCTTGTGGGCCATCCTTTTGGTAAGAAACTCTTTGGTGAACACAATCTTATCTACATGATGGGTCAAGAACACAAGAATCTCCGCCGTCGAATCGCACCCAATTTCACTCCTAAGGCGCTTTCTACATACACTGAGCTACAGCAGATTATTATCCTCAAACATCTTAAATCATGGGTTGAGATAGCCCAAGCTCAAGCCCATGAGTCTATTCCAATCCGTGTACTTTCTCGTGATATGAATTTGGAAACGTCTCAGAAAGTTTTCGTGGGACCTTATTTGGGCCTGAAAGCCCGAGAGAGATTTGAACATGATTACTTTCTCTTCAACGTTGGTCTCATGAAGCTTCCGTTTGACTTTCCGGGAACTGCGTTCCGTAATGCCAGACTCGCCGTTGACCGACTCGCCGGAACCCTAGCGGGTTGTACAGCGGCGAGTAAGGAGAAGATGGAGAAAGGTGAAGAGCCTTCTTGTCTCATTGATTTCTGGATGCAGGATACTATAAGAGAGATTGAAGAATCGAAGCTGAACGGAATAACAGTGCCACCGTTCTCCACCAACGCTGAAATCGGTGGTTATCTCTTCGATTTCCTCTTCGCAGCACAAGACGCGTCGACTTCGTCGCTTCTTTGGGCGGTGGCATTGCTGGATTCGCATCCAGAGGTTCTCGCCAGAGTGAGGGAAGAAGTTGCCGGAATCTGGACGCCGGAGTCGAATACGCTGATAACATCGGAGCAGTTAAGGGAGATGAAGTACACACAAGCGGTGGCGCGTGAGGTTATTCGTTACCGACCACCAGCTACTTTGGTGCCGCATGTTGCGGCAGAGCCGTTTCCGTTGACGGAATCTTACACGATTCCCAAAGGTGCAATAGTTTTCCCTTCGGCGTTCGAGTCATCTTTTCAGGGTTTTACTGAACCGGACCGGTTCGACCCAGACCGGTTTTCAGAAGAAAGACAAGAGGATCAAATATTTAAAAGAAACTTTCTAGCATTTGGAGCTGGGCCCCACCAATGTGTGGGCCAGAGGTACGCTTTGAATCATCTTGTGCTTTTCATTGCTATGTTCACATCGTTGATTGATTTCAAGAGAGATAGAACGGACGGTTGTGATGAGATCGTGTATGTGCCTACGATTTGTCCTAAAGATGATTGTAGGGTTTTTCTGTCTAAGCGTTGCACAAGGTTTCCTTCATTTGCTGGGGTTGTGGAGCTTGTGAAATGA
Protein sequence

>MS.gene004434.t1

MNMNAPLLLSSFSFNQLIPYLVCFILFLLLIEQISYLNKKRSIPGPSLVLPFIGNAISLVRDPTKFWEVQSTLSKSTGFSANYIIGLFIVFIRDTELSHKVFSNVKPNAFHLVGHPFGKKLFGEHNLIYMMGQEHKNLRRRIAPNFTPKALSTYTELQQIIILKHLKSWVEIAQAQAHESIPIRVLSRDMNLETSQKVFVGPYLGLKARERFEHDYFLFNVGLMKLPFDFPGTAFRNARLAVDRLAGTLAGCTAASKEKMEKGEEPSCLIDFWMQDTIREIEESKLNGITVPPFSTNAEIGGYLFDFLFAAQDASTSSLLWAVALLDSHPEVLARVREEVAGIWTPESNTLITSEQLREMKYTQAVAREVIRYRPPATLVPHVAAEPFPLTESYTIPKGAIVFPSAFESSFQGFTEPDRFDPDRFSEERQEDQIFKRNFLAFGAGPHQCVGQRYALNHLVLFIAMFTSLIDFKRDRTDGCDEIVYVPTICPKDDCRVFLSKRCTRFPSFAGVVELVK