Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004497.t1 | XP_003594049.2 | 99.3 | 153 | 1 | 0 | 1 | 153 | 1 | 153 | 1.20E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004497.t1 | Q9ZNV9 | 71.1 | 152 | 44 | 0 | 1 | 152 | 1 | 152 | 3.0e-57 | 222.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004497.t1 | B7FGU6 | 99.3 | 153 | 1 | 0 | 1 | 153 | 1 | 153 | 8.8e-79 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004497 | MS.gene75348 | PPI |
| MS.gene004497 | MS.gene60023 | PPI |
| MS.gene004497 | MS.gene89014 | PPI |
| MS.gene004497 | MS.gene034089 | PPI |
| MS.gene004497 | MS.gene026751 | PPI |
| MS.gene004497 | MS.gene83535 | PPI |
| MS.gene004497 | MS.gene067406 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004497.t1 | MTR_2g020770 | 99.346 | 153 | 1 | 0 | 1 | 153 | 1 | 153 | 5.93e-111 | 311 |
| MS.gene004497.t1 | MTR_2g020770 | 96.429 | 140 | 5 | 0 | 1 | 140 | 1 | 140 | 2.35e-97 | 277 |
| MS.gene004497.t1 | MTR_1g082290 | 56.835 | 139 | 60 | 0 | 5 | 143 | 6 | 144 | 1.09e-59 | 182 |
| MS.gene004497.t1 | MTR_7g114020 | 60.993 | 141 | 54 | 1 | 9 | 148 | 6 | 146 | 4.28e-59 | 180 |
| MS.gene004497.t1 | MTR_1g089130 | 55.944 | 143 | 63 | 0 | 5 | 147 | 3 | 145 | 1.06e-56 | 174 |
| MS.gene004497.t1 | MTR_2g100880 | 42.446 | 139 | 80 | 0 | 1 | 139 | 1 | 139 | 5.20e-39 | 129 |
| MS.gene004497.t1 | MTR_2g100900 | 42.446 | 139 | 80 | 0 | 1 | 139 | 1 | 139 | 9.47e-39 | 129 |
| MS.gene004497.t1 | MTR_2g103870 | 46.207 | 145 | 73 | 3 | 10 | 149 | 10 | 154 | 1.79e-31 | 110 |
| MS.gene004497.t1 | MTR_4g010160 | 43.478 | 115 | 63 | 2 | 37 | 149 | 28 | 142 | 7.56e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004497.t1 | AT3G21510 | 70.588 | 153 | 45 | 0 | 1 | 153 | 1 | 153 | 6.73e-71 | 210 |
| MS.gene004497.t1 | AT1G03430 | 52.632 | 152 | 71 | 1 | 3 | 153 | 6 | 157 | 7.81e-56 | 172 |
| MS.gene004497.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 5 | 155 | 3.76e-51 | 160 |
| MS.gene004497.t1 | AT3G29350 | 48.026 | 152 | 78 | 1 | 3 | 153 | 5 | 156 | 4.67e-51 | 160 |
| MS.gene004497.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 38 | 188 | 1.44e-50 | 160 |
| MS.gene004497.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 54 | 204 | 3.23e-50 | 160 |
| MS.gene004497.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 54 | 204 | 3.23e-50 | 160 |
| MS.gene004497.t1 | AT1G03430 | 51.852 | 135 | 64 | 1 | 3 | 136 | 6 | 140 | 1.82e-46 | 149 |
| MS.gene004497.t1 | AT3G16360 | 44.030 | 134 | 75 | 0 | 6 | 139 | 1 | 134 | 8.65e-39 | 129 |
| MS.gene004497.t1 | AT3G16360 | 45.000 | 120 | 66 | 0 | 19 | 138 | 8 | 127 | 1.04e-36 | 123 |
| MS.gene004497.t1 | AT3G29350 | 47.273 | 110 | 57 | 1 | 3 | 111 | 5 | 114 | 6.43e-34 | 115 |
| MS.gene004497.t1 | AT1G80100 | 49.275 | 138 | 68 | 2 | 14 | 149 | 17 | 154 | 2.32e-27 | 100 |
| MS.gene004497.t1 | AT1G80100 | 47.778 | 90 | 46 | 1 | 61 | 149 | 43 | 132 | 1.21e-21 | 84.7 |
| MS.gene004497.t1 | AT4G04402 | 33.750 | 160 | 94 | 4 | 6 | 153 | 8 | 167 | 1.32e-21 | 85.9 |
| MS.gene004497.t1 | AT1G80100 | 46.774 | 124 | 65 | 1 | 14 | 136 | 17 | 140 | 1.51e-21 | 85.5 |
Find 30 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACATGTTCACCAGTTTA+AGG | 0.220882 | 2.4:+66292126 | MS.gene004497:CDS |
| ATGGTGAACAAAGGGTTTCT+TGG | 0.350647 | 2.4:+66291674 | MS.gene004497:intron |
| ACTCAGTTCAGTTCCATCAT+AGG | 0.355461 | 2.4:-66292814 | None:intergenic |
| GCTGCTGAAGTTGGAGAAAC+TGG | 0.408482 | 2.4:-66291701 | None:intergenic |
| TTGCACTGCTACCCTTAAAC+TGG | 0.425560 | 2.4:-66292138 | None:intergenic |
| GCACATGTTCACCAGTTTAA+GGG | 0.428803 | 2.4:+66292127 | MS.gene004497:CDS |
| CTACATCAAATCCATGTTCA+TGG | 0.459422 | 2.4:+66291522 | MS.gene004497:CDS |
| CTAATTTATGCAGGTGCCGC+AGG | 0.459692 | 2.4:+66292597 | MS.gene004497:intron |
| CATCTTGTAGCTGCTGAAGT+TGG | 0.465876 | 2.4:-66291710 | None:intergenic |
| GCAGCTTGAGCAGCAGATAG+TGG | 0.473916 | 2.4:+66292774 | MS.gene004497:intron |
| CTCAGTTCAGTTCCATCATA+GGG | 0.476361 | 2.4:-66292813 | None:intergenic |
| GGGTCAGATGCGGAGACAGT+GGG | 0.506261 | 2.4:+66291495 | MS.gene004497:CDS |
| TAAGCTTGAAACATTGTTGA+GGG | 0.522076 | 2.4:+66292661 | MS.gene004497:CDS |
| TGAACAAAGGGTTTCTTGGA+TGG | 0.522720 | 2.4:+66291678 | MS.gene004497:intron |
| TGACAGCATAGGCGCACAAA+GGG | 0.523421 | 2.4:+66292348 | MS.gene004497:intron |
| GAGCAGCAGATAGTGGCAGC+TGG | 0.537850 | 2.4:+66292781 | MS.gene004497:CDS |
| TGGTCTAACCTCCATGAACA+TGG | 0.544818 | 2.4:-66291533 | None:intergenic |
| TGGTGGCTCAATCCCTATGA+TGG | 0.558041 | 2.4:+66292801 | MS.gene004497:CDS |
| TTTGTAGAGAAACATGGAGG+TGG | 0.564605 | 2.4:+66291474 | None:intergenic |
| ATAAGCTTGAAACATTGTTG+AGG | 0.584829 | 2.4:+66292660 | MS.gene004497:CDS |
| TGGGTCAGATGCGGAGACAG+TGG | 0.592973 | 2.4:+66291494 | MS.gene004497:CDS |
| TCAGATGCGGAGACAGTGGG+TGG | 0.608143 | 2.4:+66291498 | MS.gene004497:CDS |
| TTGTAGAGAAACATGGAGGT+GGG | 0.611758 | 2.4:+66291475 | None:intergenic |
| AGTTTAAGGGTAGCAGTGCA+AGG | 0.619461 | 2.4:+66292140 | MS.gene004497:CDS |
| TCACTTGCTGCAAACACCTG+CGG | 0.622315 | 2.4:-66292613 | None:intergenic |
| CAGCAGATAGTGGCAGCTGG+TGG | 0.634128 | 2.4:+66292784 | MS.gene004497:CDS |
| GAAATTGCGAAAAGCAACGC+AGG | 0.637314 | 2.4:-66292380 | None:intergenic |
| ACATGGAGGTGGGTCAGATG+CGG | 0.639294 | 2.4:+66291485 | None:intergenic |
| TTGACAGCATAGGCGCACAA+AGG | 0.651584 | 2.4:+66292347 | MS.gene004497:intron |
| CATCAAATCCATGTTCATGG+AGG | 0.716249 | 2.4:+66291525 | MS.gene004497:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTATATGATTTCTTTTA+TGG | + | chr2.4:66292742-66292761 | MS.gene004497:intron | 10.0% |
| !! | AAATCCATAAGTTTAATATA+AGG | - | chr2.4:66292720-66292739 | None:intergenic | 15.0% |
| !! | ATTAGAGAATATAATGAATT+TGG | + | chr2.4:66291871-66291890 | MS.gene004497:intron | 15.0% |
| !! | AACAAGTGAAAAAACATAAA+TGG | - | chr2.4:66292274-66292293 | None:intergenic | 20.0% |
| !! | AATAAAGTATAACCAAGAAT+TGG | - | chr2.4:66291968-66291987 | None:intergenic | 20.0% |
| !! | AGAACCTTATATTAAACTTA+TGG | + | chr2.4:66292713-66292732 | MS.gene004497:intron | 20.0% |
| !!! | TTGTTTTTCTGTTTTGTTTA+TGG | + | chr2.4:66291655-66291674 | MS.gene004497:intron | 20.0% |
| !!! | TTTTCAATTGTTGATTTTGT+TGG | + | chr2.4:66291629-66291648 | MS.gene004497:intron | 20.0% |
| !!! | TTTTGCTTTTGTTTTTGTTT+AGG | + | chr2.4:66291596-66291615 | MS.gene004497:intron | 20.0% |
| ! | AACATGAGAGAAATGAAAAA+AGG | - | chr2.4:66291847-66291866 | None:intergenic | 25.0% |
| ! | ACAACTTCAAAAACAAATTC+AGG | - | chr2.4:66291744-66291763 | None:intergenic | 25.0% |
| ! | ACCAAAAATATATAGTAGCA+TGG | - | chr2.4:66292301-66292320 | None:intergenic | 25.0% |
| ! | CTGTAGCATTTATACATTTA+AGG | - | chr2.4:66292188-66292207 | None:intergenic | 25.0% |
| ! | GTGAAATTTCTAATTTATGC+AGG | + | chr2.4:66292588-66292607 | MS.gene004497:intron | 25.0% |
| !! | ACCATGCTACTATATATTTT+TGG | + | chr2.4:66292297-66292316 | MS.gene004497:intron | 25.0% |
| !! | AGCATTTATACATTTAAGGT+TGG | - | chr2.4:66292184-66292203 | None:intergenic | 25.0% |
| !! | TGAATTTTTCGCTAATTTCA+TGG | + | chr2.4:66292556-66292575 | MS.gene004497:intron | 25.0% |
| !!! | GACTAGTTGTTTAATAGTTT+TGG | + | chr2.4:66292444-66292463 | MS.gene004497:intron | 25.0% |
| !!! | TTTTGTTTATGGTGAACAAA+GGG | + | chr2.4:66291666-66291685 | MS.gene004497:intron | 25.0% |
| AAGTATAACCAAGAATTGGT+TGG | - | chr2.4:66291964-66291983 | None:intergenic | 30.0% | |
| AGACATGATCTAATGTTGTT+GGG | + | chr2.4:66292055-66292074 | MS.gene004497:intron | 30.0% | |
| ATAAGCTTGAAACATTGTTG+AGG | + | chr2.4:66292660-66292679 | MS.gene004497:CDS | 30.0% | |
| GCTACAGAATCTGAATAAAT+CGG | + | chr2.4:66292201-66292220 | MS.gene004497:intron | 30.0% | |
| TAAGCTTGAAACATTGTTGA+GGG | + | chr2.4:66292661-66292680 | MS.gene004497:CDS | 30.0% | |
| ! | ACACATTAATTGACTCTTCT+GGG | + | chr2.4:66291562-66291581 | MS.gene004497:intron | 30.0% |
| ! | GTCAATTAATGTGTGTTTTG+TGG | - | chr2.4:66291556-66291575 | None:intergenic | 30.0% |
| !! | TTTTCTTTGATGATTCTGAG+AGG | + | chr2.4:66291769-66291788 | MS.gene004497:CDS | 30.0% |
| !!! | GTTTTGTTTATGGTGAACAA+AGG | + | chr2.4:66291665-66291684 | MS.gene004497:intron | 30.0% |
| CTACATCAAATCCATGTTCA+TGG | + | chr2.4:66291522-66291541 | MS.gene004497:CDS | 35.0% | |
| GACATGATCTAATGTTGTTG+GGG | + | chr2.4:66292056-66292075 | MS.gene004497:intron | 35.0% | |
| GAGACATGATCTAATGTTGT+TGG | + | chr2.4:66292054-66292073 | MS.gene004497:intron | 35.0% | |
| GTATGTTTGATTGACAGCAT+AGG | + | chr2.4:66292337-66292356 | MS.gene004497:intron | 35.0% | |
| TCTCCTGAATGATTCATTCT+AGG | - | chr2.4:66291926-66291945 | None:intergenic | 35.0% | |
| ! | CACACATTAATTGACTCTTC+TGG | + | chr2.4:66291561-66291580 | MS.gene004497:intron | 35.0% |
| ATGGTGAACAAAGGGTTTCT+TGG | + | chr2.4:66291674-66291693 | MS.gene004497:intron | 40.0% | |
| CAGCTAGTGCAATAGTTTGA+AGG | + | chr2.4:66292483-66292502 | MS.gene004497:intron | 40.0% | |
| CATCAAATCCATGTTCATGG+AGG | + | chr2.4:66291525-66291544 | MS.gene004497:CDS | 40.0% | |
| CCACCTAGAATGAATCATTC+AGG | + | chr2.4:66291920-66291939 | MS.gene004497:intron | 40.0% | |
| CCTGAATGATTCATTCTAGG+TGG | - | chr2.4:66291923-66291942 | None:intergenic | 40.0% | |
| CTTTACTGCTTAGATGCTAG+AGG | + | chr2.4:66292525-66292544 | MS.gene004497:intron | 40.0% | |
| GCACATGTTCACCAGTTTAA+GGG | + | chr2.4:66292127-66292146 | MS.gene004497:CDS | 40.0% | |
| TGAACAAAGGGTTTCTTGGA+TGG | + | chr2.4:66291678-66291697 | MS.gene004497:intron | 40.0% | |
| TGCACATGTTCACCAGTTTA+AGG | + | chr2.4:66292126-66292145 | MS.gene004497:CDS | 40.0% | |
| !! | CTCTCTTGCTATTAATCACC+CGG | - | chr2.4:66292035-66292054 | None:intergenic | 40.0% |
| AGTTTAAGGGTAGCAGTGCA+AGG | + | chr2.4:66292140-66292159 | MS.gene004497:CDS | 45.0% | |
| ATCATTCAGGAGAACCCTCT+TGG | + | chr2.4:66291933-66291952 | MS.gene004497:intron | 45.0% | |
| CATCTTGTAGCTGCTGAAGT+TGG | - | chr2.4:66291713-66291732 | None:intergenic | 45.0% | |
| CTATTAATCACCCGGTTCTG+GGG | - | chr2.4:66292027-66292046 | None:intergenic | 45.0% | |
| GAAATTGCGAAAAGCAACGC+AGG | - | chr2.4:66292383-66292402 | None:intergenic | 45.0% | |
| GCTATTAATCACCCGGTTCT+GGG | - | chr2.4:66292028-66292047 | None:intergenic | 45.0% | |
| TCATTCAGGAGAACCCTCTT+GGG | + | chr2.4:66291934-66291953 | MS.gene004497:intron | 45.0% | |
| TGCTATTAATCACCCGGTTC+TGG | - | chr2.4:66292029-66292048 | None:intergenic | 45.0% | |
| TGGTCTAACCTCCATGAACA+TGG | - | chr2.4:66291536-66291555 | None:intergenic | 45.0% | |
| TTGCACTGCTACCCTTAAAC+TGG | - | chr2.4:66292141-66292160 | None:intergenic | 45.0% | |
| TTTACCTCTCGAGCGAACTT+CGG | + | chr2.4:66291986-66292005 | MS.gene004497:intron | 45.0% | |
| ! | GTTGGGGTTTTACATGTGCA+GGG | + | chr2.4:66292072-66292091 | MS.gene004497:intron | 45.0% |
| ! | TGTTGGGGTTTTACATGTGC+AGG | + | chr2.4:66292071-66292090 | MS.gene004497:intron | 45.0% |
| GCTGCTGAAGTTGGAGAAAC+TGG | - | chr2.4:66291704-66291723 | None:intergenic | 50.0% | |
| TAATCCGAAGTTCGCTCGAG+AGG | - | chr2.4:66291993-66292012 | None:intergenic | 50.0% | |
| TGACAGCATAGGCGCACAAA+GGG | + | chr2.4:66292348-66292367 | MS.gene004497:intron | 50.0% | |
| TGGGTGGACCAACCAATTCT+TGG | + | chr2.4:66291953-66291972 | MS.gene004497:intron | 50.0% | |
| TGGTGGCTCAATCCCTATGA+TGG | + | chr2.4:66292801-66292820 | MS.gene004497:CDS | 50.0% | |
| TTGACAGCATAGGCGCACAA+AGG | + | chr2.4:66292347-66292366 | MS.gene004497:intron | 50.0% | |
| ! | AATTGGTTGGTCCACCCAAG+AGG | - | chr2.4:66291951-66291970 | None:intergenic | 50.0% |
| ! | ATTGGTTGGTCCACCCAAGA+GGG | - | chr2.4:66291950-66291969 | None:intergenic | 50.0% |
| ! | CTAATTTATGCAGGTGCCGC+AGG | + | chr2.4:66292597-66292616 | MS.gene004497:intron | 50.0% |
| ! | TCACTTGCTGCAAACACCTG+CGG | - | chr2.4:66292616-66292635 | None:intergenic | 50.0% |
| CGAGCGAACTTCGGATTACC+AGG | + | chr2.4:66291995-66292014 | MS.gene004497:intron | 55.0% | |
| GCAGCTTGAGCAGCAGATAG+TGG | + | chr2.4:66292774-66292793 | MS.gene004497:intron | 55.0% | |
| TTCAGGAGAACCCTCTTGGG+TGG | + | chr2.4:66291937-66291956 | MS.gene004497:intron | 55.0% | |
| !! | AATCACCCGGTTCTGGGGAA+GGG | - | chr2.4:66292022-66292041 | None:intergenic | 55.0% |
| !! | TAATCACCCGGTTCTGGGGA+AGG | - | chr2.4:66292023-66292042 | None:intergenic | 55.0% |
| GGGTCAGATGCGGAGACAGT+GGG | + | chr2.4:66291495-66291514 | MS.gene004497:CDS | 60.0% | |
| TCAGATGCGGAGACAGTGGG+TGG | + | chr2.4:66291498-66291517 | MS.gene004497:CDS | 60.0% | |
| TGGGTCAGATGCGGAGACAG+TGG | + | chr2.4:66291494-66291513 | MS.gene004497:CDS | 60.0% | |
| ! | CAGCAGATAGTGGCAGCTGG+TGG | + | chr2.4:66292784-66292803 | MS.gene004497:CDS | 60.0% |
| ! | GAGCAGCAGATAGTGGCAGC+TGG | + | chr2.4:66292781-66292800 | MS.gene004497:CDS | 60.0% |
| !! | ATCACCCGGTTCTGGGGAAG+GGG | - | chr2.4:66292021-66292040 | None:intergenic | 60.0% |
| CAGGCCCCTTCCCCAGAACC+GGG | + | chr2.4:66292014-66292033 | MS.gene004497:intron | 70.0% | |
| CCAGGCCCCTTCCCCAGAAC+CGG | + | chr2.4:66292013-66292032 | MS.gene004497:intron | 70.0% | |
| !! | CCGGTTCTGGGGAAGGGGCC+TGG | - | chr2.4:66292016-66292035 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 66291487 | 66292834 | 66291487 | ID=MS.gene004497 |
| chr2.4 | mRNA | 66291487 | 66292834 | 66291487 | ID=MS.gene004497.t1;Parent=MS.gene004497 |
| chr2.4 | exon | 66291487 | 66291546 | 66291487 | ID=MS.gene004497.t1.exon1;Parent=MS.gene004497.t1 |
| chr2.4 | CDS | 66291487 | 66291546 | 66291487 | ID=cds.MS.gene004497.t1;Parent=MS.gene004497.t1 |
| chr2.4 | exon | 66291687 | 66291817 | 66291687 | ID=MS.gene004497.t1.exon2;Parent=MS.gene004497.t1 |
| chr2.4 | CDS | 66291687 | 66291817 | 66291687 | ID=cds.MS.gene004497.t1;Parent=MS.gene004497.t1 |
| chr2.4 | exon | 66292093 | 66292161 | 66292093 | ID=MS.gene004497.t1.exon3;Parent=MS.gene004497.t1 |
| chr2.4 | CDS | 66292093 | 66292161 | 66292093 | ID=cds.MS.gene004497.t1;Parent=MS.gene004497.t1 |
| chr2.4 | exon | 66292354 | 66292425 | 66292354 | ID=MS.gene004497.t1.exon4;Parent=MS.gene004497.t1 |
| chr2.4 | CDS | 66292354 | 66292425 | 66292354 | ID=cds.MS.gene004497.t1;Parent=MS.gene004497.t1 |
| chr2.4 | exon | 66292610 | 66292682 | 66292610 | ID=MS.gene004497.t1.exon5;Parent=MS.gene004497.t1 |
| chr2.4 | CDS | 66292610 | 66292682 | 66292610 | ID=cds.MS.gene004497.t1;Parent=MS.gene004497.t1 |
| chr2.4 | exon | 66292778 | 66292834 | 66292778 | ID=MS.gene004497.t1.exon6;Parent=MS.gene004497.t1 |
| chr2.4 | CDS | 66292778 | 66292834 | 66292778 | ID=cds.MS.gene004497.t1;Parent=MS.gene004497.t1 |
| Gene Sequence |
| Protein sequence |
