Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034089.t1 | XP_003604376.2 | 98.6 | 145 | 2 | 0 | 1 | 145 | 1 | 145 | 7.20E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034089.t1 | Q9SSC9 | 77.5 | 142 | 32 | 0 | 1 | 142 | 13 | 154 | 6.2e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034089.t1 | G7JGY0 | 98.6 | 145 | 2 | 0 | 1 | 145 | 1 | 145 | 5.2e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene004497 | MS.gene034089 | PPI |
| MS.gene034089 | MS.gene00955 | PPI |
| MS.gene034089 | MS.gene002811 | PPI |
| MS.gene034089 | MS.gene049808 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034089.t1 | MTR_4g010160 | 98.621 | 145 | 2 | 0 | 1 | 145 | 1 | 145 | 1.88e-104 | 294 |
| MS.gene034089.t1 | MTR_2g103870 | 88.112 | 143 | 17 | 0 | 1 | 143 | 13 | 155 | 4.33e-85 | 246 |
| MS.gene034089.t1 | MTR_7g114020 | 47.794 | 136 | 70 | 1 | 6 | 141 | 12 | 146 | 4.42e-38 | 127 |
| MS.gene034089.t1 | MTR_1g082290 | 45.161 | 124 | 67 | 1 | 8 | 131 | 18 | 140 | 6.95e-36 | 121 |
| MS.gene034089.t1 | MTR_1g089130 | 48.462 | 130 | 64 | 2 | 8 | 136 | 15 | 142 | 1.12e-33 | 115 |
| MS.gene034089.t1 | MTR_2g100900 | 41.935 | 124 | 71 | 1 | 8 | 131 | 17 | 139 | 4.65e-30 | 106 |
| MS.gene034089.t1 | MTR_2g020770 | 48.148 | 135 | 68 | 2 | 8 | 142 | 17 | 149 | 4.78e-30 | 106 |
| MS.gene034089.t1 | MTR_2g100880 | 41.935 | 124 | 71 | 1 | 8 | 131 | 17 | 139 | 8.73e-30 | 105 |
| MS.gene034089.t1 | MTR_2g020770 | 47.154 | 123 | 64 | 1 | 8 | 130 | 17 | 138 | 7.18e-27 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034089.t1 | AT1G80100 | 77.465 | 142 | 32 | 0 | 1 | 142 | 13 | 154 | 1.64e-59 | 181 |
| MS.gene034089.t1 | AT1G80100 | 76.562 | 128 | 30 | 0 | 1 | 128 | 13 | 140 | 1.21e-49 | 156 |
| MS.gene034089.t1 | AT1G80100 | 65.493 | 142 | 27 | 1 | 1 | 142 | 13 | 132 | 3.35e-49 | 154 |
| MS.gene034089.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 19 | 151 | 3.54e-38 | 127 |
| MS.gene034089.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 52 | 184 | 1.79e-37 | 126 |
| MS.gene034089.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 68 | 200 | 4.23e-37 | 126 |
| MS.gene034089.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 68 | 200 | 4.23e-37 | 126 |
| MS.gene034089.t1 | AT1G03430 | 43.284 | 134 | 75 | 1 | 8 | 141 | 20 | 152 | 2.41e-36 | 122 |
| MS.gene034089.t1 | AT3G29350 | 42.748 | 131 | 75 | 0 | 8 | 138 | 19 | 149 | 6.79e-36 | 121 |
| MS.gene034089.t1 | AT1G03430 | 42.975 | 121 | 69 | 0 | 8 | 128 | 20 | 140 | 1.09e-33 | 116 |
| MS.gene034089.t1 | AT3G21510 | 48.092 | 131 | 67 | 1 | 8 | 138 | 17 | 146 | 2.46e-32 | 112 |
| MS.gene034089.t1 | AT3G16360 | 41.045 | 134 | 78 | 1 | 8 | 141 | 12 | 144 | 2.33e-31 | 109 |
| MS.gene034089.t1 | AT3G16360 | 42.500 | 120 | 68 | 1 | 11 | 130 | 9 | 127 | 1.23e-28 | 102 |
| MS.gene034089.t1 | AT3G29350 | 43.750 | 96 | 54 | 0 | 8 | 103 | 19 | 114 | 2.57e-25 | 93.6 |
| MS.gene034089.t1 | AT4G04402 | 30.986 | 142 | 83 | 5 | 12 | 141 | 24 | 162 | 2.59e-13 | 63.9 |
Find 28 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTGATTTGAATCAGTTTA+TGG | 0.142653 | 4.4:-89075451 | MS.gene034089:CDS |
| CAGGATGGAGAGAGAATTTA+TGG | 0.196613 | 4.4:-89075490 | MS.gene034089:CDS |
| ATTTATGGACTATAAGAAAA+TGG | 0.240158 | 4.4:-89075475 | MS.gene034089:CDS |
| TTTATGGACTATAAGAAAAT+GGG | 0.347973 | 4.4:-89075474 | MS.gene034089:CDS |
| GATTGAACAGCAACGAGCTT+TGG | 0.383140 | 4.4:-89074570 | MS.gene034089:CDS |
| ATTGATTTGAATCAGTTTAT+GGG | 0.393080 | 4.4:-89075450 | MS.gene034089:CDS |
| ATGTCTCATCTTGTAGCTGC+TGG | 0.413122 | 4.4:+89075694 | None:intergenic |
| ACGAATCTGAGAAGCTTCTC+AGG | 0.417214 | 4.4:-89075632 | MS.gene034089:CDS |
| CTTCAGAGCGTTGGAAATGC+TGG | 0.427599 | 4.4:-89075029 | MS.gene034089:CDS |
| TGAGAAGCTTCTCAGATTCG+TGG | 0.428630 | 4.4:+89075634 | None:intergenic |
| ACAACCTCAGAGACAAAGTT+TGG | 0.431005 | 4.4:+89075669 | None:intergenic |
| CAGCAACGAGCTTTGGCAGC+TGG | 0.433005 | 4.4:-89074563 | MS.gene034089:CDS |
| ATACTTTATTCATTCTGCAC+TGG | 0.452579 | 4.4:+89074530 | None:intergenic |
| ACTGAACAGAACAACCGTGC+TGG | 0.501598 | 4.4:-89075366 | MS.gene034089:CDS |
| GGAAGAGGAAGGAGAGGAGG+CGG | 0.501898 | 4.4:+89075954 | None:intergenic |
| TTGCAGATGCTTCAGAGCGT+TGG | 0.502520 | 4.4:-89075038 | MS.gene034089:intron |
| CCTCTCCTTCCTCTTCCACC+AGG | 0.516920 | 4.4:-89075948 | MS.gene034089:intron |
| ACATACGTACCTGGTGGAAG+AGG | 0.530544 | 4.4:+89075939 | None:intergenic |
| CTGAACAGAACAACCGTGCT+GGG | 0.535597 | 4.4:-89075365 | MS.gene034089:intron |
| ATGGGAAGCAGCTCAAGCAT+TGG | 0.551335 | 4.4:-89075432 | MS.gene034089:CDS |
| TTATGGACTATAAGAAAATG+GGG | 0.562353 | 4.4:-89075473 | MS.gene034089:CDS |
| GGTGGAAGAGGAAGGAGAGG+AGG | 0.572375 | 4.4:+89075951 | None:intergenic |
| CCTGGTGGAAGAGGAAGGAG+AGG | 0.593472 | 4.4:+89075948 | None:intergenic |
| ATCACCAAACTTTGTCTCTG+AGG | 0.606924 | 4.4:-89075673 | MS.gene034089:CDS |
| GAGCTTTGGCAGCTGGAGTG+AGG | 0.614608 | 4.4:-89074556 | MS.gene034089:CDS |
| ACGTACCTGGTGGAAGAGGA+AGG | 0.619941 | 4.4:+89075943 | None:intergenic |
| CTGTTCAGTAGCGACACGAA+AGG | 0.656974 | 4.4:+89075380 | None:intergenic |
| GTATAGGCACCCACCCAGCA+CGG | 0.691862 | 4.4:+89075352 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAATTATATTAAATGAAAT+AGG | - | chr4.4:89075630-89075649 | MS.gene034089:CDS | 10.0% |
| !!! | ATATATAGTTTTTTTCTTAT+AGG | - | chr4.4:89074749-89074768 | MS.gene034089:intron | 10.0% |
| !!! | TATATAGTTTTTTTCTTATA+GGG | - | chr4.4:89074750-89074769 | MS.gene034089:intron | 10.0% |
| !! | TAAAAAAACAAAAATTAGCA+AGG | + | chr4.4:89075835-89075854 | None:intergenic | 15.0% |
| !! | TATTAAGATAATTACTTGTT+AGG | + | chr4.4:89074926-89074945 | None:intergenic | 15.0% |
| !!! | ATTTTTACATGAATTTATTC+AGG | - | chr4.4:89074985-89075004 | MS.gene034089:CDS | 15.0% |
| !! | ATTTAGAGACAAAATTATAG+TGG | - | chr4.4:89075782-89075801 | MS.gene034089:intron | 20.0% |
| !! | TGATAAACATTAATAATGAG+AGG | + | chr4.4:89075881-89075900 | None:intergenic | 20.0% |
| !! | TTAGTGTATGTTTGATATTA+AGG | - | chr4.4:89075212-89075231 | MS.gene034089:intron | 20.0% |
| !!! | ATTGATTTGAATCAGTTTAT+GGG | - | chr4.4:89075044-89075063 | MS.gene034089:intron | 20.0% |
| !!! | ATTTATGGACTATAAGAAAA+TGG | - | chr4.4:89075019-89075038 | MS.gene034089:CDS | 20.0% |
| !!! | TAGTTTTTAAAAAACCATAG+TGG | - | chr4.4:89075295-89075314 | MS.gene034089:intron | 20.0% |
| !!! | TTTATGGACTATAAGAAAAT+GGG | - | chr4.4:89075020-89075039 | MS.gene034089:CDS | 20.0% |
| ! | CAAAACTTTGTAGATATACT+AGG | + | chr4.4:89075574-89075593 | None:intergenic | 25.0% |
| ! | TTACATGAATTTATTCAGGA+TGG | - | chr4.4:89074989-89075008 | MS.gene034089:CDS | 25.0% |
| ! | TTATTCGTGCATTCTTTATT+CGG | - | chr4.4:89075172-89075191 | MS.gene034089:intron | 25.0% |
| !! | TTATGGACTATAAGAAAATG+GGG | - | chr4.4:89075021-89075040 | MS.gene034089:CDS | 25.0% |
| !!! | GATTGATTTGAATCAGTTTA+TGG | - | chr4.4:89075043-89075062 | MS.gene034089:intron | 25.0% |
| !!! | TTTTTTCTTATAGGGAGTAT+TGG | - | chr4.4:89074758-89074777 | MS.gene034089:intron | 25.0% |
| AAAGAATGCACGAATAAAGA+GGG | + | chr4.4:89075169-89075188 | None:intergenic | 30.0% | |
| ATTAGCAAGGAAATTTAGAG+AGG | + | chr4.4:89075822-89075841 | None:intergenic | 30.0% | |
| GTGTATGTTTGATATTAAGG+TGG | - | chr4.4:89075215-89075234 | MS.gene034089:intron | 30.0% | |
| TAAAGAATGCACGAATAAAG+AGG | + | chr4.4:89075170-89075189 | None:intergenic | 30.0% | |
| TACAAAATCACGATGAAACA+TGG | + | chr4.4:89075255-89075274 | None:intergenic | 30.0% | |
| TTAGCAAGGAAATTTAGAGA+GGG | + | chr4.4:89075821-89075840 | None:intergenic | 30.0% | |
| ! | ATGCTAGTTAGTGTTCTTTT+TGG | - | chr4.4:89074641-89074660 | MS.gene034089:intron | 30.0% |
| AAAATCACAACGATCCACTA+TGG | + | chr4.4:89075312-89075331 | None:intergenic | 35.0% | |
| CTACATATCACAGTGCATAA+TGG | + | chr4.4:89075364-89075383 | None:intergenic | 35.0% | |
| ! | CGTGATTTTGTAGATGTAGA+AGG | - | chr4.4:89075263-89075282 | MS.gene034089:intron | 35.0% |
| ACAACCTCAGAGACAAAGTT+TGG | + | chr4.4:89074828-89074847 | None:intergenic | 40.0% | |
| AGGTTGAGTGCAACTTTACT+TGG | + | chr4.4:89075534-89075553 | None:intergenic | 40.0% | |
| ATAGAAAATAGACGTGCGCA+TGG | - | chr4.4:89075674-89075693 | MS.gene034089:CDS | 40.0% | |
| ATCACCAAACTTTGTCTCTG+AGG | - | chr4.4:89074821-89074840 | MS.gene034089:intron | 40.0% | |
| CAGGATGGAGAGAGAATTTA+TGG | - | chr4.4:89075004-89075023 | MS.gene034089:CDS | 40.0% | |
| ! | CACGAATAAAGAGGGAGTAT+AGG | + | chr4.4:89075161-89075180 | None:intergenic | 40.0% |
| ! | GATAGTGTTTGATAGCACGA+TGG | + | chr4.4:89075342-89075361 | None:intergenic | 40.0% |
| ! | TGTTTGATAGCACGATGGAT+AGG | + | chr4.4:89075337-89075356 | None:intergenic | 40.0% |
| ACGAATCTGAGAAGCTTCTC+AGG | - | chr4.4:89074862-89074881 | MS.gene034089:intron | 45.0% | |
| AGCATCTGCAATTGGTTGGA+TGG | + | chr4.4:89075447-89075466 | None:intergenic | 45.0% | |
| ATGTCTCATCTTGTAGCTGC+TGG | + | chr4.4:89074803-89074822 | None:intergenic | 45.0% | |
| GATTGAACAGCAACGAGCTT+TGG | - | chr4.4:89075924-89075943 | MS.gene034089:intron | 45.0% | |
| GCATCTGCAATTGGTTGGAT+GGG | + | chr4.4:89075446-89075465 | None:intergenic | 45.0% | |
| TGAGAAGCTTCTCAGATTCG+TGG | + | chr4.4:89074863-89074882 | None:intergenic | 45.0% | |
| ! | AGGCTGCTAGCTAGCTTAAT+AGG | + | chr4.4:89075554-89075573 | None:intergenic | 45.0% |
| ! | CTGAAGCATCTGCAATTGGT+TGG | + | chr4.4:89075451-89075470 | None:intergenic | 45.0% |
| ! | TCTGAAACATACGTACCTGG+TGG | + | chr4.4:89074564-89074583 | None:intergenic | 45.0% |
| !! | GTCTCTGAAACATACGTACC+TGG | + | chr4.4:89074567-89074586 | None:intergenic | 45.0% |
| ACATACGTACCTGGTGGAAG+AGG | + | chr4.4:89074558-89074577 | None:intergenic | 50.0% | |
| ACTGAACAGAACAACCGTGC+TGG | - | chr4.4:89075128-89075147 | MS.gene034089:intron | 50.0% | |
| ATGGGAAGCAGCTCAAGCAT+TGG | - | chr4.4:89075062-89075081 | MS.gene034089:intron | 50.0% | |
| CTGAACAGAACAACCGTGCT+GGG | - | chr4.4:89075129-89075148 | MS.gene034089:intron | 50.0% | |
| CTGTTCAGTAGCGACACGAA+AGG | + | chr4.4:89075117-89075136 | None:intergenic | 50.0% | |
| TTGCAGATGCTTCAGAGCGT+TGG | - | chr4.4:89075456-89075475 | MS.gene034089:CDS | 50.0% | |
| !! | CGCTCTGAAGCATCTGCAAT+TGG | + | chr4.4:89075455-89075474 | None:intergenic | 50.0% |
| !! | CTTCAGAGCGTTGGAAATGC+TGG | - | chr4.4:89075465-89075484 | MS.gene034089:CDS | 50.0% |
| AACAGAACAACCGTGCTGGG+TGG | - | chr4.4:89075132-89075151 | MS.gene034089:intron | 55.0% | |
| ACAGAACAACCGTGCTGGGT+GGG | - | chr4.4:89075133-89075152 | MS.gene034089:intron | 55.0% | |
| ACGTACCTGGTGGAAGAGGA+AGG | + | chr4.4:89074554-89074573 | None:intergenic | 55.0% | |
| CCTCTCCTTCCTCTTCCACC+AGG | - | chr4.4:89074546-89074565 | MS.gene034089:CDS | 60.0% | |
| CCTGGTGGAAGAGGAAGGAG+AGG | + | chr4.4:89074549-89074568 | None:intergenic | 60.0% | |
| GGAAGAGGAAGGAGAGGAGG+CGG | + | chr4.4:89074543-89074562 | None:intergenic | 60.0% | |
| GGTGGAAGAGGAAGGAGAGG+AGG | + | chr4.4:89074546-89074565 | None:intergenic | 60.0% | |
| GTATAGGCACCCACCCAGCA+CGG | + | chr4.4:89075145-89075164 | None:intergenic | 60.0% | |
| ! | CAGCAACGAGCTTTGGCAGC+TGG | - | chr4.4:89075931-89075950 | MS.gene034089:intron | 60.0% |
| ! | GAGCTTTGGCAGCTGGAGTG+AGG | - | chr4.4:89075938-89075957 | MS.gene034089:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 89074535 | 89075981 | 89074535 | ID=MS.gene034089 |
| chr4.4 | mRNA | 89074535 | 89075981 | 89074535 | ID=MS.gene034089.t1;Parent=MS.gene034089 |
| chr4.4 | exon | 89075949 | 89075981 | 89075949 | ID=MS.gene034089.t1.exon1;Parent=MS.gene034089.t1 |
| chr4.4 | CDS | 89075949 | 89075981 | 89075949 | ID=cds.MS.gene034089.t1;Parent=MS.gene034089.t1 |
| chr4.4 | exon | 89075615 | 89075745 | 89075615 | ID=MS.gene034089.t1.exon2;Parent=MS.gene034089.t1 |
| chr4.4 | CDS | 89075615 | 89075745 | 89075615 | ID=cds.MS.gene034089.t1;Parent=MS.gene034089.t1 |
| chr4.4 | exon | 89075366 | 89075509 | 89075366 | ID=MS.gene034089.t1.exon3;Parent=MS.gene034089.t1 |
| chr4.4 | CDS | 89075366 | 89075509 | 89075366 | ID=cds.MS.gene034089.t1;Parent=MS.gene034089.t1 |
| chr4.4 | exon | 89074982 | 89075054 | 89074982 | ID=MS.gene034089.t1.exon4;Parent=MS.gene034089.t1 |
| chr4.4 | CDS | 89074982 | 89075054 | 89074982 | ID=cds.MS.gene034089.t1;Parent=MS.gene034089.t1 |
| chr4.4 | exon | 89074535 | 89074591 | 89074535 | ID=MS.gene034089.t1.exon5;Parent=MS.gene034089.t1 |
| chr4.4 | CDS | 89074535 | 89074591 | 89074535 | ID=cds.MS.gene034089.t1;Parent=MS.gene034089.t1 |
| Gene Sequence |
| Protein sequence |
