Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026751.t1 | XP_003604376.2 | 97.9 | 145 | 3 | 0 | 1 | 145 | 1 | 145 | 2.10E-72 | 281.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026751.t1 | Q9SSC9 | 77.5 | 142 | 32 | 0 | 1 | 142 | 13 | 154 | 6.2e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026751.t1 | G7JGY0 | 97.9 | 145 | 3 | 0 | 1 | 145 | 1 | 145 | 1.5e-72 | 281.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene026751 | MS.gene00955 | PPI |
| MS.gene004497 | MS.gene026751 | PPI |
| MS.gene026751 | MS.gene002811 | PPI |
| MS.gene026751 | MS.gene049808 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026751.t1 | MTR_4g010160 | 97.931 | 145 | 3 | 0 | 1 | 145 | 1 | 145 | 6.51e-104 | 293 |
| MS.gene026751.t1 | MTR_2g103870 | 87.413 | 143 | 18 | 0 | 1 | 143 | 13 | 155 | 1.69e-84 | 244 |
| MS.gene026751.t1 | MTR_7g114020 | 47.794 | 136 | 70 | 1 | 6 | 141 | 12 | 146 | 5.38e-38 | 126 |
| MS.gene026751.t1 | MTR_1g082290 | 45.161 | 124 | 67 | 1 | 8 | 131 | 18 | 140 | 9.85e-36 | 121 |
| MS.gene026751.t1 | MTR_1g089130 | 48.462 | 130 | 64 | 2 | 8 | 136 | 15 | 142 | 8.27e-34 | 116 |
| MS.gene026751.t1 | MTR_2g100900 | 41.935 | 124 | 71 | 1 | 8 | 131 | 17 | 139 | 4.45e-30 | 106 |
| MS.gene026751.t1 | MTR_2g020770 | 48.148 | 135 | 68 | 2 | 8 | 142 | 17 | 149 | 5.94e-30 | 106 |
| MS.gene026751.t1 | MTR_2g100880 | 41.935 | 124 | 71 | 1 | 8 | 131 | 17 | 139 | 8.00e-30 | 105 |
| MS.gene026751.t1 | MTR_2g020770 | 47.154 | 123 | 64 | 1 | 8 | 130 | 17 | 138 | 9.83e-27 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026751.t1 | AT1G80100 | 77.465 | 142 | 32 | 0 | 1 | 142 | 13 | 154 | 1.61e-59 | 181 |
| MS.gene026751.t1 | AT1G80100 | 76.562 | 128 | 30 | 0 | 1 | 128 | 13 | 140 | 1.29e-49 | 156 |
| MS.gene026751.t1 | AT1G80100 | 65.493 | 142 | 27 | 1 | 1 | 142 | 13 | 132 | 3.39e-49 | 154 |
| MS.gene026751.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 19 | 151 | 3.32e-38 | 127 |
| MS.gene026751.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 52 | 184 | 1.65e-37 | 126 |
| MS.gene026751.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 68 | 200 | 4.00e-37 | 126 |
| MS.gene026751.t1 | AT5G39340 | 46.269 | 134 | 71 | 1 | 8 | 141 | 68 | 200 | 4.00e-37 | 126 |
| MS.gene026751.t1 | AT1G03430 | 43.284 | 134 | 75 | 1 | 8 | 141 | 20 | 152 | 2.16e-36 | 122 |
| MS.gene026751.t1 | AT3G29350 | 42.748 | 131 | 75 | 0 | 8 | 138 | 19 | 149 | 6.16e-36 | 121 |
| MS.gene026751.t1 | AT1G03430 | 42.975 | 121 | 69 | 0 | 8 | 128 | 20 | 140 | 9.75e-34 | 116 |
| MS.gene026751.t1 | AT3G21510 | 47.328 | 131 | 68 | 1 | 8 | 138 | 17 | 146 | 9.79e-32 | 110 |
| MS.gene026751.t1 | AT3G16360 | 41.045 | 134 | 78 | 1 | 8 | 141 | 12 | 144 | 2.07e-31 | 110 |
| MS.gene026751.t1 | AT3G16360 | 42.500 | 120 | 68 | 1 | 11 | 130 | 9 | 127 | 1.08e-28 | 102 |
| MS.gene026751.t1 | AT3G29350 | 43.750 | 96 | 54 | 0 | 8 | 103 | 19 | 114 | 2.35e-25 | 93.6 |
| MS.gene026751.t1 | AT4G04402 | 31.690 | 142 | 82 | 5 | 12 | 141 | 24 | 162 | 1.75e-13 | 64.3 |
Find 27 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTGATTTGAATCAGTTTA+TGG | 0.142653 | 4.1:-85672481 | MS.gene026751:CDS |
| CAGGATGGAGAGAGAATTTA+TGG | 0.196613 | 4.1:-85672520 | MS.gene026751:CDS |
| ATTTATGGACTATAAGAAAA+TGG | 0.240158 | 4.1:-85672505 | MS.gene026751:CDS |
| TTTATGGACTATAAGAAAAT+GGG | 0.347973 | 4.1:-85672504 | MS.gene026751:CDS |
| GATTGAACAGCAACGAGCTT+TGG | 0.383140 | 4.1:-85671597 | MS.gene026751:CDS |
| ATTGATTTGAATCAGTTTAT+GGG | 0.393080 | 4.1:-85672480 | MS.gene026751:CDS |
| ATGTCTCATCTTGTAGCTGC+TGG | 0.413122 | 4.1:+85672724 | None:intergenic |
| ACGAATCTGAGAAGCTTCTC+AGG | 0.417214 | 4.1:-85672662 | MS.gene026751:CDS |
| CTTCAGAGCGTTGGAAATGC+TGG | 0.427599 | 4.1:-85672074 | MS.gene026751:CDS |
| TGAGAAGCTTCTCAGATTCG+TGG | 0.428630 | 4.1:+85672664 | None:intergenic |
| ACAACCTCAGAGACAAAGTT+TGG | 0.431005 | 4.1:+85672699 | None:intergenic |
| CAGCAACGAGCTTTGGCAGC+TGG | 0.433005 | 4.1:-85671590 | MS.gene026751:CDS |
| ATACTTTATTCATTCTGCAC+TGG | 0.452579 | 4.1:+85671557 | None:intergenic |
| ACTGAAAAGAACAACCGTGC+TGG | 0.494777 | 4.1:-85672396 | MS.gene026751:CDS |
| GGAAGAGGAAGGAGAGGAGG+CGG | 0.501898 | 4.1:+85672982 | None:intergenic |
| TTGCAGATGCTTCAGAGCGT+TGG | 0.502520 | 4.1:-85672083 | MS.gene026751:intron |
| CCTCTCCTTCCTCTTCCACC+AGG | 0.516920 | 4.1:-85672976 | MS.gene026751:intron |
| CTGAAAAGAACAACCGTGCT+GGG | 0.528776 | 4.1:-85672395 | MS.gene026751:intron |
| ACATACGTACCTGGTGGAAG+AGG | 0.530544 | 4.1:+85672967 | None:intergenic |
| ATGGGAAGCAGCTCAAGCAT+TGG | 0.551335 | 4.1:-85672462 | MS.gene026751:CDS |
| TTATGGACTATAAGAAAATG+GGG | 0.562353 | 4.1:-85672503 | MS.gene026751:CDS |
| GGTGGAAGAGGAAGGAGAGG+AGG | 0.572375 | 4.1:+85672979 | None:intergenic |
| CCTGGTGGAAGAGGAAGGAG+AGG | 0.593472 | 4.1:+85672976 | None:intergenic |
| ATCACCAAACTTTGTCTCTG+AGG | 0.606924 | 4.1:-85672703 | MS.gene026751:CDS |
| GAGCTTTGGCAGCTGGAGTG+AGG | 0.614608 | 4.1:-85671583 | MS.gene026751:CDS |
| ACGTACCTGGTGGAAGAGGA+AGG | 0.619941 | 4.1:+85672971 | None:intergenic |
| GTATAGGCACCCACCCAGCA+CGG | 0.691862 | 4.1:+85672382 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATAGTTTTTTTCTTAT+AGG | - | chr4.1:85671774-85671793 | MS.gene026751:intron | 10.0% |
| !!! | TATATAGTTTTTTTCTTATA+GGG | - | chr4.1:85671775-85671794 | MS.gene026751:intron | 10.0% |
| !! | AAAACCAAAATTAATTAGTA+AGG | + | chr4.1:85672858-85672877 | None:intergenic | 15.0% |
| !! | TACAAAATCAAGATAAATTA+TGG | + | chr4.1:85672280-85672299 | None:intergenic | 15.0% |
| !! | TATTAAGATAATTACTTGTT+AGG | + | chr4.1:85671951-85671970 | None:intergenic | 15.0% |
| !!! | AATATGATTTATTGATTCAA+TGG | - | chr4.1:85672688-85672707 | MS.gene026751:CDS | 15.0% |
| !!! | ATTTTTACATGAATTTATTC+AGG | - | chr4.1:85672010-85672029 | MS.gene026751:intron | 15.0% |
| !!! | TTTTCCTTACTAATTAATTT+TGG | - | chr4.1:85672851-85672870 | MS.gene026751:intron | 15.0% |
| !! | ATTTAACGACAAAATTATAG+TGG | - | chr4.1:85672804-85672823 | MS.gene026751:intron | 20.0% |
| !! | TAAAACTTTGTAGATATACT+AGG | + | chr4.1:85672584-85672603 | None:intergenic | 20.0% |
| !! | TATGAGATTTATTGATATCA+TGG | + | chr4.1:85672649-85672668 | None:intergenic | 20.0% |
| !! | TGATAAACATAAATAATGAG+AGG | + | chr4.1:85672909-85672928 | None:intergenic | 20.0% |
| !! | TTAATGTATGTTTGATATCA+AGG | - | chr4.1:85672237-85672256 | MS.gene026751:intron | 20.0% |
| !! | TTAGTAAGGAAAATTTAGAA+AGG | + | chr4.1:85672844-85672863 | None:intergenic | 20.0% |
| !!! | ATTGATTTGAATCAGTTTAT+GGG | - | chr4.1:85672069-85672088 | MS.gene026751:CDS | 20.0% |
| !!! | ATTTATCTTGATTTTGTAGA+AGG | - | chr4.1:85672282-85672301 | MS.gene026751:intron | 20.0% |
| !!! | ATTTATGGACTATAAGAAAA+TGG | - | chr4.1:85672044-85672063 | MS.gene026751:CDS | 20.0% |
| !!! | ATTTTGGTTTTTTTTTAGTG+TGG | - | chr4.1:85672867-85672886 | MS.gene026751:intron | 20.0% |
| !!! | TTTATGGACTATAAGAAAAT+GGG | - | chr4.1:85672045-85672064 | MS.gene026751:CDS | 20.0% |
| !!! | TTTTGGTTTTTTTTTAGTGT+GGG | - | chr4.1:85672868-85672887 | MS.gene026751:intron | 20.0% |
| ! | TTACATGAATTTATTCAGGA+TGG | - | chr4.1:85672014-85672033 | MS.gene026751:intron | 25.0% |
| ! | TTATTCGTGCATTCTTTATT+GGG | - | chr4.1:85672197-85672216 | MS.gene026751:intron | 25.0% |
| ! | TTTATTCGTGCATTCTTTAT+TGG | - | chr4.1:85672196-85672215 | MS.gene026751:intron | 25.0% |
| !! | TTATGGACTATAAGAAAATG+GGG | - | chr4.1:85672046-85672065 | MS.gene026751:CDS | 25.0% |
| !!! | GATTGATTTGAATCAGTTTA+TGG | - | chr4.1:85672068-85672087 | MS.gene026751:CDS | 25.0% |
| !!! | TTTTTTCTTATAGGGAGTAT+TGG | - | chr4.1:85671783-85671802 | MS.gene026751:intron | 25.0% |
| AAAGAATGCACGAATAAAGA+GGG | + | chr4.1:85672194-85672213 | None:intergenic | 30.0% | |
| GTTTGATATCAAGGTGAATA+TGG | - | chr4.1:85672246-85672265 | MS.gene026751:intron | 30.0% | |
| TAAAGAATGCACGAATAAAG+AGG | + | chr4.1:85672195-85672214 | None:intergenic | 30.0% | |
| TAGTGTAGCACAAAATACTA+GGG | - | chr4.1:85672381-85672400 | MS.gene026751:intron | 30.0% | |
| ! | ATGCTAGTTAGTGTTCTTTT+TGG | - | chr4.1:85671669-85671688 | MS.gene026751:intron | 30.0% |
| CTAGTGTAGCACAAAATACT+AGG | - | chr4.1:85672380-85672399 | MS.gene026751:intron | 35.0% | |
| GATAGTGTTTGATATCACCA+TGG | + | chr4.1:85672361-85672380 | None:intergenic | 35.0% | |
| AAGTCTTGCACATTGCAACA+AGG | - | chr4.1:85672614-85672633 | MS.gene026751:intron | 40.0% | |
| ACAACCTCAGAGACAAAGTT+TGG | + | chr4.1:85671853-85671872 | None:intergenic | 40.0% | |
| AGGTTGAGTGCAACTTTACT+TGG | + | chr4.1:85672544-85672563 | None:intergenic | 40.0% | |
| ATCACCAAACTTTGTCTCTG+AGG | - | chr4.1:85671846-85671865 | MS.gene026751:intron | 40.0% | |
| CAGGATGGAGAGAGAATTTA+TGG | - | chr4.1:85672029-85672048 | MS.gene026751:CDS | 40.0% | |
| GTAGAAAATAGACGTCTGTG+TGG | - | chr4.1:85672710-85672729 | MS.gene026751:CDS | 40.0% | |
| ! | CACGAATAAAGAGGGAGTAT+AGG | + | chr4.1:85672186-85672205 | None:intergenic | 40.0% |
| !! | CTGTGATTTTGACGTATCCA+TGG | - | chr4.1:85672341-85672360 | MS.gene026751:intron | 40.0% |
| ACGAATCTGAGAAGCTTCTC+AGG | - | chr4.1:85671887-85671906 | MS.gene026751:intron | 45.0% | |
| ACTGAAAAGAACAACCGTGC+TGG | - | chr4.1:85672153-85672172 | MS.gene026751:intron | 45.0% | |
| AGCATCTGCAATTGGTTGGA+TGG | + | chr4.1:85672457-85672476 | None:intergenic | 45.0% | |
| ATGTCTCATCTTGTAGCTGC+TGG | + | chr4.1:85671828-85671847 | None:intergenic | 45.0% | |
| CTGAAAAGAACAACCGTGCT+GGG | - | chr4.1:85672154-85672173 | MS.gene026751:intron | 45.0% | |
| GATTGAACAGCAACGAGCTT+TGG | - | chr4.1:85672952-85672971 | MS.gene026751:intron | 45.0% | |
| GCATCTGCAATTGGTTGGAT+GGG | + | chr4.1:85672456-85672475 | None:intergenic | 45.0% | |
| TGAGAAGCTTCTCAGATTCG+TGG | + | chr4.1:85671888-85671907 | None:intergenic | 45.0% | |
| ! | AGGCTGCTAGCTAGCTTAAT+AGG | + | chr4.1:85672564-85672583 | None:intergenic | 45.0% |
| ! | CTGAAGCATCTGCAATTGGT+TGG | + | chr4.1:85672461-85672480 | None:intergenic | 45.0% |
| ! | CTTTTCAGTAGCGACACGAA+AGG | + | chr4.1:85672142-85672161 | None:intergenic | 45.0% |
| ! | TCTGAAACATACGTACCTGG+TGG | + | chr4.1:85671591-85671610 | None:intergenic | 45.0% |
| !! | GTCTCTGAAACATACGTACC+TGG | + | chr4.1:85671594-85671613 | None:intergenic | 45.0% |
| AAAAGAACAACCGTGCTGGG+TGG | - | chr4.1:85672157-85672176 | MS.gene026751:intron | 50.0% | |
| AAAGAACAACCGTGCTGGGT+GGG | - | chr4.1:85672158-85672177 | MS.gene026751:intron | 50.0% | |
| ACATACGTACCTGGTGGAAG+AGG | + | chr4.1:85671585-85671604 | None:intergenic | 50.0% | |
| ATGGGAAGCAGCTCAAGCAT+TGG | - | chr4.1:85672087-85672106 | MS.gene026751:intron | 50.0% | |
| TTGCAGATGCTTCAGAGCGT+TGG | - | chr4.1:85672466-85672485 | MS.gene026751:CDS | 50.0% | |
| !! | CGCTCTGAAGCATCTGCAAT+TGG | + | chr4.1:85672465-85672484 | None:intergenic | 50.0% |
| !! | CTTCAGAGCGTTGGAAATGC+TGG | - | chr4.1:85672475-85672494 | MS.gene026751:CDS | 50.0% |
| ACGTACCTGGTGGAAGAGGA+AGG | + | chr4.1:85671581-85671600 | None:intergenic | 55.0% | |
| CCTCTCCTTCCTCTTCCACC+AGG | - | chr4.1:85671573-85671592 | MS.gene026751:CDS | 60.0% | |
| CCTGGTGGAAGAGGAAGGAG+AGG | + | chr4.1:85671576-85671595 | None:intergenic | 60.0% | |
| GGAAGAGGAAGGAGAGGAGG+CGG | + | chr4.1:85671570-85671589 | None:intergenic | 60.0% | |
| GGTGGAAGAGGAAGGAGAGG+AGG | + | chr4.1:85671573-85671592 | None:intergenic | 60.0% | |
| GTATAGGCACCCACCCAGCA+CGG | + | chr4.1:85672170-85672189 | None:intergenic | 60.0% | |
| ! | CAGCAACGAGCTTTGGCAGC+TGG | - | chr4.1:85672959-85672978 | MS.gene026751:intron | 60.0% |
| ! | GAGCTTTGGCAGCTGGAGTG+AGG | - | chr4.1:85672966-85672985 | MS.gene026751:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 85671562 | 85673009 | 85671562 | ID=MS.gene026751 |
| chr4.1 | mRNA | 85671562 | 85673009 | 85671562 | ID=MS.gene026751.t1;Parent=MS.gene026751 |
| chr4.1 | exon | 85672977 | 85673009 | 85672977 | ID=MS.gene026751.t1.exon1;Parent=MS.gene026751.t1 |
| chr4.1 | CDS | 85672977 | 85673009 | 85672977 | ID=cds.MS.gene026751.t1;Parent=MS.gene026751.t1 |
| chr4.1 | exon | 85672645 | 85672775 | 85672645 | ID=MS.gene026751.t1.exon2;Parent=MS.gene026751.t1 |
| chr4.1 | CDS | 85672645 | 85672775 | 85672645 | ID=cds.MS.gene026751.t1;Parent=MS.gene026751.t1 |
| chr4.1 | exon | 85672396 | 85672539 | 85672396 | ID=MS.gene026751.t1.exon3;Parent=MS.gene026751.t1 |
| chr4.1 | CDS | 85672396 | 85672539 | 85672396 | ID=cds.MS.gene026751.t1;Parent=MS.gene026751.t1 |
| chr4.1 | exon | 85672027 | 85672099 | 85672027 | ID=MS.gene026751.t1.exon4;Parent=MS.gene026751.t1 |
| chr4.1 | CDS | 85672027 | 85672099 | 85672027 | ID=cds.MS.gene026751.t1;Parent=MS.gene026751.t1 |
| chr4.1 | exon | 85671562 | 85671618 | 85671562 | ID=MS.gene026751.t1.exon5;Parent=MS.gene026751.t1 |
| chr4.1 | CDS | 85671562 | 85671618 | 85671562 | ID=cds.MS.gene026751.t1;Parent=MS.gene026751.t1 |
| Gene Sequence |
| Protein sequence |
