Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067406.t1 | XP_003597907.2 | 99.4 | 158 | 1 | 0 | 1 | 158 | 1 | 158 | 3.00E-80 | 307.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067406.t1 | Q9SSC9 | 77.3 | 154 | 35 | 0 | 1 | 154 | 1 | 154 | 2.0e-61 | 236.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067406.t1 | G7II71 | 99.4 | 158 | 1 | 0 | 1 | 158 | 1 | 158 | 2.2e-80 | 307.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067406 | MS.gene002811 | PPI |
| MS.gene067406 | MS.gene049808 | PPI |
| MS.gene004497 | MS.gene067406 | PPI |
| MS.gene067406 | MS.gene00955 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067406.t1 | MTR_2g103870 | 99.367 | 158 | 1 | 0 | 1 | 158 | 1 | 158 | 1.71e-115 | 323 |
| MS.gene067406.t1 | MTR_4g010160 | 86.713 | 143 | 19 | 0 | 13 | 155 | 1 | 143 | 2.81e-79 | 231 |
| MS.gene067406.t1 | MTR_7g114020 | 46.269 | 134 | 71 | 1 | 20 | 153 | 14 | 146 | 7.40e-37 | 124 |
| MS.gene067406.t1 | MTR_1g082290 | 45.161 | 124 | 67 | 1 | 20 | 143 | 18 | 140 | 8.04e-36 | 121 |
| MS.gene067406.t1 | MTR_1g089130 | 46.429 | 140 | 70 | 2 | 13 | 148 | 4 | 142 | 2.31e-35 | 120 |
| MS.gene067406.t1 | MTR_2g020770 | 46.207 | 145 | 73 | 3 | 10 | 154 | 10 | 149 | 3.58e-31 | 110 |
| MS.gene067406.t1 | MTR_2g100900 | 41.935 | 124 | 71 | 1 | 20 | 143 | 17 | 139 | 9.82e-30 | 106 |
| MS.gene067406.t1 | MTR_2g100880 | 41.935 | 124 | 71 | 1 | 20 | 143 | 17 | 139 | 1.42e-29 | 105 |
| MS.gene067406.t1 | MTR_2g020770 | 44.361 | 133 | 70 | 2 | 10 | 142 | 10 | 138 | 3.47e-27 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067406.t1 | AT1G80100 | 77.273 | 154 | 35 | 0 | 1 | 154 | 1 | 154 | 1.38e-68 | 205 |
| MS.gene067406.t1 | AT1G80100 | 75.714 | 140 | 34 | 0 | 1 | 140 | 1 | 140 | 5.52e-58 | 178 |
| MS.gene067406.t1 | AT1G80100 | 66.883 | 154 | 29 | 1 | 1 | 154 | 1 | 132 | 7.78e-58 | 177 |
| MS.gene067406.t1 | AT5G39340 | 41.722 | 151 | 83 | 2 | 7 | 153 | 2 | 151 | 1.54e-36 | 124 |
| MS.gene067406.t1 | AT5G39340 | 44.030 | 134 | 74 | 1 | 20 | 153 | 52 | 184 | 8.48e-36 | 122 |
| MS.gene067406.t1 | AT1G03430 | 42.537 | 134 | 76 | 1 | 20 | 153 | 20 | 152 | 1.38e-35 | 121 |
| MS.gene067406.t1 | AT5G39340 | 44.030 | 134 | 74 | 1 | 20 | 153 | 68 | 200 | 2.15e-35 | 122 |
| MS.gene067406.t1 | AT5G39340 | 44.030 | 134 | 74 | 1 | 20 | 153 | 68 | 200 | 2.15e-35 | 122 |
| MS.gene067406.t1 | AT3G29350 | 41.221 | 131 | 77 | 0 | 20 | 150 | 19 | 149 | 8.59e-35 | 119 |
| MS.gene067406.t1 | AT1G03430 | 42.149 | 121 | 70 | 0 | 20 | 140 | 20 | 140 | 3.95e-33 | 115 |
| MS.gene067406.t1 | AT3G16360 | 41.791 | 134 | 77 | 1 | 20 | 153 | 12 | 144 | 1.37e-32 | 113 |
| MS.gene067406.t1 | AT3G21510 | 47.328 | 131 | 68 | 1 | 20 | 150 | 17 | 146 | 2.65e-32 | 112 |
| MS.gene067406.t1 | AT3G16360 | 43.333 | 120 | 67 | 1 | 23 | 142 | 9 | 127 | 9.91e-30 | 105 |
| MS.gene067406.t1 | AT3G29350 | 41.667 | 96 | 56 | 0 | 20 | 115 | 19 | 114 | 8.22e-24 | 90.1 |
| MS.gene067406.t1 | AT4G04402 | 29.012 | 162 | 98 | 6 | 4 | 153 | 6 | 162 | 1.23e-13 | 65.1 |
Find 31 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCACTCTGGATGCTTGGTT+TGG | 0.259246 | 2.4:+1651125 | None:intergenic |
| CCACTCTGGATGCTTGGTTT+GGG | 0.300260 | 2.4:+1651126 | None:intergenic |
| CCTTGCATTTCTCTTCCATC+AGG | 0.306041 | 2.4:+1651182 | MS.gene067406:CDS |
| ACAACTTCAGAGACAAAGTT+AGG | 0.349554 | 2.4:-1651258 | None:intergenic |
| CAGCAACGTGCTTTGGCAGC+TGG | 0.382396 | 2.4:+1652279 | MS.gene067406:CDS |
| GATAGAGCAGCAACGTGCTT+TGG | 0.394138 | 2.4:+1652272 | MS.gene067406:intron |
| CTTGCATTTCTCTTCCATCA+GGG | 0.403749 | 2.4:+1651183 | MS.gene067406:CDS |
| ATACATTTGAATCAATTCAT+TGG | 0.432634 | 2.4:+1651658 | MS.gene067406:CDS |
| TTTGGGTGCGGACCTCTTGT+GGG | 0.440442 | 2.4:+1651143 | MS.gene067406:CDS |
| GGTCTTCCACTCTGGATGCT+TGG | 0.445346 | 2.4:+1651120 | None:intergenic |
| TTTGCGAGCGTTGGAAATGC+TGG | 0.454165 | 2.4:+1652088 | MS.gene067406:CDS |
| GTTTGGGTGCGGACCTCTTG+TGG | 0.459527 | 2.4:+1651142 | MS.gene067406:CDS |
| ATTTATTGATGCTGCTGCAT+AGG | 0.460477 | 2.4:-1652312 | None:intergenic |
| TTACAGCAATGATCTGAGAT+TGG | 0.475206 | 2.4:-1651316 | None:intergenic |
| TTGTAGATGTTTGCGAGCGT+TGG | 0.477843 | 2.4:+1652079 | MS.gene067406:intron |
| TTTATTGATGCTGCTGCATA+GGG | 0.480181 | 2.4:-1652311 | None:intergenic |
| TCTGAGCAAGACAACCGTGC+TGG | 0.483071 | 2.4:+1651742 | MS.gene067406:CDS |
| TGCATTTCTCTTCCATCAGG+GGG | 0.500140 | 2.4:+1651185 | MS.gene067406:CDS |
| CTGAGCAAGACAACCGTGCT+GGG | 0.507329 | 2.4:+1651743 | MS.gene067406:CDS |
| CAACTTCAGAGACAAAGTTA+GGG | 0.524139 | 2.4:-1651257 | None:intergenic |
| CTGGATGCTTGGTTTGGGTG+CGG | 0.535283 | 2.4:+1651131 | None:intergenic |
| TTGCATTTCTCTTCCATCAG+GGG | 0.542436 | 2.4:+1651184 | MS.gene067406:CDS |
| ATTGGTAGTAGCTCAAGCAT+TGG | 0.586684 | 2.4:+1651676 | MS.gene067406:CDS |
| TGATCATTGTAGAATGGAGA+GGG | 0.606921 | 2.4:+1651609 | MS.gene067406:intron |
| GATGCATGTCGGCCCACAAG+AGG | 0.616168 | 2.4:-1651155 | None:intergenic |
| TGCAAGGAGACGATGCATGT+CGG | 0.635385 | 2.4:-1651166 | None:intergenic |
| CCTGATGGAAGAGAAATGCA+AGG | 0.646278 | 2.4:-1651182 | None:intergenic |
| GTGATCATTGTAGAATGGAG+AGG | 0.646641 | 2.4:+1651608 | MS.gene067406:intron |
| AAAATGGAACGAACCCAGCA+CGG | 0.649795 | 2.4:-1651756 | None:intergenic |
| TTCAGAGACAAAGTTAGGGG+AGG | 0.659336 | 2.4:-1651253 | None:intergenic |
| AACTTCAGAGACAAAGTTAG+GGG | 0.715591 | 2.4:-1651256 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTAATTTACGATAAATTATA+TGG | + | chr2.4:1652240-1652259 | MS.gene067406:intron | 15.0% |
| !! | ATACATTTGAATCAATTCAT+TGG | + | chr2.4:1651658-1651677 | MS.gene067406:CDS | 20.0% |
| !! | GATCATAGTATAGAAATATA+AGG | - | chr2.4:1651358-1651377 | None:intergenic | 20.0% |
| ! | CAATGATACAAAATGCATAA+TGG | + | chr2.4:1652006-1652025 | MS.gene067406:intron | 25.0% |
| ! | TAACTAGTAACTATATCTGT+AGG | - | chr2.4:1651442-1651461 | None:intergenic | 25.0% |
| !!! | TTTTAAGAGGGAATGAAAAA+TGG | - | chr2.4:1651775-1651794 | None:intergenic | 25.0% |
| AGATGCAATAGAACTTTACT+TGG | - | chr2.4:1652157-1652176 | None:intergenic | 30.0% | |
| CACTACACTAACATTGATAT+TGG | - | chr2.4:1651499-1651518 | None:intergenic | 30.0% | |
| AACTTCAGAGACAAAGTTAG+GGG | - | chr2.4:1651259-1651278 | None:intergenic | 35.0% | |
| ACAACTTCAGAGACAAAGTT+AGG | - | chr2.4:1651261-1651280 | None:intergenic | 35.0% | |
| ATATTTCTCCTATGAAGCAC+CGG | - | chr2.4:1651550-1651569 | None:intergenic | 35.0% | |
| CAACTTCAGAGACAAAGTTA+GGG | - | chr2.4:1651260-1651279 | None:intergenic | 35.0% | |
| TACAAGTTAGAACTCATCAC+TGG | - | chr2.4:1652046-1652065 | None:intergenic | 35.0% | |
| TATATCTGTAGGTGTCTTGT+CGG | - | chr2.4:1651431-1651450 | None:intergenic | 35.0% | |
| TGATCATTGTAGAATGGAGA+GGG | + | chr2.4:1651609-1651628 | MS.gene067406:intron | 35.0% | |
| ! | AGTAGTGCACGAATTTTAAG+AGG | - | chr2.4:1651788-1651807 | None:intergenic | 35.0% |
| ! | TTACAGCAATGATCTGAGAT+TGG | - | chr2.4:1651319-1651338 | None:intergenic | 35.0% |
| !! | AAAGTGCGTGCATTTTTAAG+TGG | - | chr2.4:1651912-1651931 | None:intergenic | 35.0% |
| !! | GTAGTGCACGAATTTTAAGA+GGG | - | chr2.4:1651787-1651806 | None:intergenic | 35.0% |
| !!! | AAGTGCGTGCATTTTTAAGT+GGG | - | chr2.4:1651911-1651930 | None:intergenic | 35.0% |
| !!! | GTGCATTTTTAAGTGGGAAA+AGG | - | chr2.4:1651905-1651924 | None:intergenic | 35.0% |
| CTTGCATTTCTCTTCCATCA+GGG | + | chr2.4:1651183-1651202 | MS.gene067406:CDS | 40.0% | |
| GCTCGCAAACATCTACAATT+AGG | - | chr2.4:1652077-1652096 | None:intergenic | 40.0% | |
| GTGATCATTGTAGAATGGAG+AGG | + | chr2.4:1651608-1651627 | MS.gene067406:intron | 40.0% | |
| TACTTCTGATACAAGACGTC+TGG | + | chr2.4:1651402-1651421 | MS.gene067406:intron | 40.0% | |
| TGCGTGTGATCATTGTAGAA+TGG | + | chr2.4:1651603-1651622 | MS.gene067406:intron | 40.0% | |
| TTGCATTTCTCTTCCATCAG+GGG | + | chr2.4:1651184-1651203 | MS.gene067406:CDS | 40.0% | |
| ! | ATTGGTAGTAGCTCAAGCAT+TGG | + | chr2.4:1651676-1651695 | MS.gene067406:CDS | 40.0% |
| ! | TATTTCTCCTATGAAGCACC+GGG | - | chr2.4:1651549-1651568 | None:intergenic | 40.0% |
| AAAATGGAACGAACCCAGCA+CGG | - | chr2.4:1651759-1651778 | None:intergenic | 45.0% | |
| CCTGATGGAAGAGAAATGCA+AGG | - | chr2.4:1651185-1651204 | None:intergenic | 45.0% | |
| CCTTGCATTTCTCTTCCATC+AGG | + | chr2.4:1651182-1651201 | MS.gene067406:CDS | 45.0% | |
| TGCATTTCTCTTCCATCAGG+GGG | + | chr2.4:1651185-1651204 | MS.gene067406:CDS | 45.0% | |
| TTCAGAGACAAAGTTAGGGG+AGG | - | chr2.4:1651256-1651275 | None:intergenic | 45.0% | |
| TTCTGATACAAGACGTCTGG+TGG | + | chr2.4:1651405-1651424 | MS.gene067406:intron | 45.0% | |
| TTGTAGATGTTTGCGAGCGT+TGG | + | chr2.4:1652079-1652098 | MS.gene067406:intron | 45.0% | |
| ! | AAAGGAAAGTGTGACAGTGG+AGG | - | chr2.4:1651887-1651906 | None:intergenic | 45.0% |
| ! | AAGGAAAGTGTGACAGTGGA+GGG | - | chr2.4:1651886-1651905 | None:intergenic | 45.0% |
| ! | GGAAAAGGAAAGTGTGACAG+TGG | - | chr2.4:1651890-1651909 | None:intergenic | 45.0% |
| !! | ACTATATTATATTATTATTA+TGG | - | chr2.4:1652211-1652230 | None:intergenic | 5.0% |
| !!! | AATTTTTTATCAATAAAATA+AGG | + | chr2.4:1651827-1651846 | MS.gene067406:intron | 5.0% |
| GATAGAGCAGCAACGTGCTT+TGG | + | chr2.4:1652272-1652291 | MS.gene067406:intron | 50.0% | |
| GTATGTCTGTGTGTCTGTCC+CGG | + | chr2.4:1651528-1651547 | MS.gene067406:intron | 50.0% | |
| TGCAAGGAGACGATGCATGT+CGG | - | chr2.4:1651169-1651188 | None:intergenic | 50.0% | |
| !! | TTTGCGAGCGTTGGAAATGC+TGG | + | chr2.4:1652088-1652107 | MS.gene067406:CDS | 50.0% |
| AGTGTGACAGTGGAGGGTTG+CGG | - | chr2.4:1651880-1651899 | None:intergenic | 55.0% | |
| CTGAGCAAGACAACCGTGCT+GGG | + | chr2.4:1651743-1651762 | MS.gene067406:CDS | 55.0% | |
| TCTGAGCAAGACAACCGTGC+TGG | + | chr2.4:1651742-1651761 | MS.gene067406:CDS | 55.0% | |
| TGTCTGTCCCGGTGCTTCAT+AGG | + | chr2.4:1651539-1651558 | MS.gene067406:intron | 55.0% | |
| TTTGGGTGCGGACCTCTTGT+GGG | + | chr2.4:1651143-1651162 | MS.gene067406:CDS | 55.0% | |
| ! | TCTCTTCCATCAGGGGGTGT+TGG | + | chr2.4:1651191-1651210 | MS.gene067406:CDS | 55.0% |
| CAGCAACGTGCTTTGGCAGC+TGG | + | chr2.4:1652279-1652298 | MS.gene067406:CDS | 60.0% | |
| GATGCATGTCGGCCCACAAG+AGG | - | chr2.4:1651158-1651177 | None:intergenic | 60.0% | |
| GCTCATCCAACACCCCCTGA+TGG | - | chr2.4:1651200-1651219 | None:intergenic | 60.0% | |
| GTTTGGGTGCGGACCTCTTG+TGG | + | chr2.4:1651142-1651161 | MS.gene067406:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 1651135 | 1652332 | 1651135 | ID=MS.gene067406 |
| chr2.4 | mRNA | 1651135 | 1652332 | 1651135 | ID=MS.gene067406.t1;Parent=MS.gene067406 |
| chr2.4 | exon | 1651135 | 1651334 | 1651135 | ID=MS.gene067406.t1.exon1;Parent=MS.gene067406.t1 |
| chr2.4 | CDS | 1651135 | 1651334 | 1651135 | ID=cds.MS.gene067406.t1;Parent=MS.gene067406.t1 |
| chr2.4 | exon | 1651621 | 1651764 | 1651621 | ID=MS.gene067406.t1.exon2;Parent=MS.gene067406.t1 |
| chr2.4 | CDS | 1651621 | 1651764 | 1651621 | ID=cds.MS.gene067406.t1;Parent=MS.gene067406.t1 |
| chr2.4 | exon | 1652085 | 1652157 | 1652085 | ID=MS.gene067406.t1.exon3;Parent=MS.gene067406.t1 |
| chr2.4 | CDS | 1652085 | 1652157 | 1652085 | ID=cds.MS.gene067406.t1;Parent=MS.gene067406.t1 |
| chr2.4 | exon | 1652273 | 1652332 | 1652273 | ID=MS.gene067406.t1.exon4;Parent=MS.gene067406.t1 |
| chr2.4 | CDS | 1652273 | 1652332 | 1652273 | ID=cds.MS.gene067406.t1;Parent=MS.gene067406.t1 |
| Gene Sequence |
| Protein sequence |
