AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004518

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004518.t1 MTR_6g051425 28.521 284 161 6 170 412 7 289 1.32e-30 124
MS.gene004518.t1 MTR_4g067290 43.119 109 50 1 179 275 55 163 4.61e-23 99.4
MS.gene004518.t1 MTR_4g067290 33.333 150 70 3 28 150 55 201 2.06e-16 79.7
MS.gene004518.t1 MTR_4g067290 43.119 109 50 1 179 275 55 163 4.71e-23 99.0
MS.gene004518.t1 MTR_4g067290 33.784 148 70 3 28 150 55 199 2.04e-16 79.7
MS.gene004518.t1 MTR_4g067290 43.119 109 50 1 179 275 73 181 6.80e-23 99.0
MS.gene004518.t1 MTR_4g067290 33.784 148 70 3 28 150 73 217 3.65e-16 79.3
MS.gene004518.t1 MTR_4g067290 43.119 109 50 1 179 275 75 183 7.02e-23 99.0
MS.gene004518.t1 MTR_4g067290 33.784 148 70 3 28 150 75 219 3.54e-16 79.7
MS.gene004518.t1 MTR_4g067290 43.119 109 50 1 179 275 75 183 7.54e-23 99.0
MS.gene004518.t1 MTR_4g067290 33.333 150 70 3 28 150 75 221 3.46e-16 79.7
MS.gene004518.t1 MTR_4g067290 43.119 109 50 1 179 275 115 223 1.39e-22 99.4
MS.gene004518.t1 MTR_4g067290 33.333 150 70 3 28 150 115 261 6.29e-16 79.7
MS.gene004518.t1 MTR_2g098920 41.284 109 52 1 179 275 79 187 1.14e-21 95.9
MS.gene004518.t1 MTR_2g098920 41.176 102 48 1 28 117 79 180 1.87e-17 83.6
MS.gene004518.t1 MTR_2g098880 36.735 147 67 3 26 152 58 198 4.68e-19 87.8
MS.gene004518.t1 MTR_2g098880 33.019 106 56 1 178 268 59 164 2.64e-13 70.9
MS.gene004518.t1 MTR_2g098880 36.735 147 67 3 26 152 70 210 5.00e-19 88.2
MS.gene004518.t1 MTR_2g098880 33.019 106 56 1 178 268 71 176 2.77e-13 71.2
MS.gene004518.t1 MTR_3g006370 35.659 129 69 1 179 293 49 177 5.37e-18 84.3
MS.gene004518.t1 MTR_3g006370 39.423 104 49 1 28 117 49 152 5.79e-18 84.3
MS.gene004518.t1 MTR_7g061980 35.135 111 54 4 24 119 5 112 1.18e-12 65.9
MS.gene004518.t1 MTR_6g022070 26.506 166 116 3 439 600 1 164 6.12e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004518.t1 AT3G06250 59.792 771 290 9 1 758 1 764 0.0 930
MS.gene004518.t1 AT3G06250 59.792 771 290 9 1 758 1 764 0.0 930
MS.gene004518.t1 AT3G06250 59.792 771 290 9 1 758 1 764 0.0 930
MS.gene004518.t1 AT5G18960 56.978 781 307 10 1 758 14 788 0.0 889
MS.gene004518.t1 AT5G18960 56.978 781 307 10 1 758 14 788 0.0 889
MS.gene004518.t1 AT4G38180 36.160 625 349 11 178 754 71 693 2.20e-129 404
MS.gene004518.t1 AT4G38180 33.974 156 78 4 20 158 63 210 3.03e-14 77.4
MS.gene004518.t1 AT2G27110 37.261 628 364 12 149 754 16 635 5.43e-122 386
MS.gene004518.t1 AT2G27110 37.261 628 364 12 149 754 16 635 5.43e-122 386
MS.gene004518.t1 AT2G27110 40.652 460 258 6 308 754 33 490 5.45e-108 345
MS.gene004518.t1 AT4G15090 32.736 614 367 10 178 749 49 658 4.30e-106 343
MS.gene004518.t1 AT4G15090 32.736 614 367 10 178 749 49 658 4.30e-106 343
MS.gene004518.t1 AT4G15090 32.736 614 367 10 178 749 49 658 4.30e-106 343
MS.gene004518.t1 AT4G15090 32.736 614 367 10 178 749 49 658 4.30e-106 343
MS.gene004518.t1 AT4G15090 32.736 614 367 10 178 749 49 658 4.30e-106 343
MS.gene004518.t1 AT4G38170 40.136 441 252 7 323 755 21 457 2.63e-104 330
MS.gene004518.t1 AT1G52520 32.846 615 369 14 180 754 85 695 2.08e-100 325
MS.gene004518.t1 AT1G52520 29.530 149 90 3 29 165 85 230 8.50e-11 65.9
MS.gene004518.t1 AT3G22170 31.240 605 367 10 178 744 68 661 5.30e-100 328
MS.gene004518.t1 AT3G22170 31.240 605 367 10 178 744 68 661 5.30e-100 328
MS.gene004518.t1 AT1G76320 33.759 548 326 10 185 699 2 545 8.67e-90 296
MS.gene004518.t1 AT1G76320 33.759 548 326 10 185 699 2 545 3.41e-89 296
MS.gene004518.t1 AT1G76320 33.759 548 326 10 185 699 2 545 3.41e-89 296
MS.gene004518.t1 AT1G76320 33.759 548 326 10 185 699 2 545 3.47e-89 296
MS.gene004518.t1 AT2G32250 31.852 540 322 8 183 699 41 557 3.70e-86 289
MS.gene004518.t1 AT2G32250 31.852 540 322 8 183 699 41 557 1.35e-85 288
MS.gene004518.t1 AT2G32250 31.852 540 322 8 183 699 41 557 1.35e-85 288
MS.gene004518.t1 AT2G32250 31.852 540 322 8 183 699 41 557 1.35e-85 288
MS.gene004518.t1 AT2G32250 31.852 540 322 8 183 699 41 557 1.44e-85 288
MS.gene004518.t1 AT2G32250 31.852 540 322 8 183 699 41 557 1.44e-85 288
MS.gene004518.t1 AT2G32250 31.784 538 321 8 185 699 2 516 3.38e-85 286
MS.gene004518.t1 AT2G32250 31.784 538 321 8 185 699 2 516 4.68e-85 286
MS.gene004518.t1 AT1G80010 28.708 627 391 12 180 754 95 717 1.26e-84 284
MS.gene004518.t1 AT1G80010 31.579 114 69 1 16 120 82 195 2.12e-11 68.2
MS.gene004518.t1 AT4G19990 29.244 595 361 11 168 723 15 588 3.65e-76 261
MS.gene004518.t1 AT4G19990 29.244 595 361 11 168 723 15 588 3.65e-76 261
MS.gene004518.t1 AT4G19990 29.244 595 361 11 168 723 15 588 6.07e-76 261
MS.gene004518.t1 AT4G19990 29.244 595 361 11 168 723 29 602 6.50e-76 261
MS.gene004518.t1 AT4G19990 29.244 595 361 11 168 723 15 588 6.63e-76 261
MS.gene004518.t1 AT4G19990 29.244 595 361 11 168 723 15 588 6.63e-76 261
MS.gene004518.t1 AT4G19990 30.200 500 321 8 237 723 47 531 2.92e-68 238
MS.gene004518.t1 AT5G28530 28.970 573 337 14 180 693 55 616 2.65e-59 213
MS.gene004518.t1 AT5G28530 28.621 573 335 14 180 693 55 612 4.73e-59 213
MS.gene004518.t1 AT5G28530 28.621 573 335 14 180 693 55 612 4.73e-59 213
MS.gene004518.t1 AT1G10240 27.634 579 350 15 172 695 40 604 4.24e-58 210
MS.gene004518.t1 AT1G10240 27.634 579 350 15 172 695 40 604 4.24e-58 210
MS.gene004518.t1 AT5G28530 25.741 540 334 13 180 693 55 553 1.43e-45 173
MS.gene004518.t1 AT5G28530 25.370 540 332 13 180 693 55 549 2.05e-45 172
MS.gene004518.t1 AT3G59470 46.789 109 46 1 179 275 69 177 5.79e-24 102
MS.gene004518.t1 AT3G59470 34.161 161 73 3 14 148 62 215 4.50e-19 88.2
MS.gene004518.t1 AT3G59470 46.789 109 46 1 179 275 69 177 5.79e-24 102
MS.gene004518.t1 AT3G59470 34.161 161 73 3 14 148 62 215 4.50e-19 88.2
MS.gene004518.t1 AT3G59470 46.789 109 46 1 179 275 81 189 7.01e-24 102
MS.gene004518.t1 AT3G59470 34.161 161 73 3 14 148 74 227 5.29e-19 88.2
MS.gene004518.t1 AT3G07500 38.532 109 54 1 173 268 26 134 3.73e-19 87.4
MS.gene004518.t1 AT3G07500 39.806 103 49 1 28 117 32 134 3.64e-16 78.6
MS.gene004518.t1 AT4G12850 42.574 101 48 1 28 118 10 110 1.93e-18 82.8
MS.gene004518.t1 AT4G12850 42.982 114 52 2 179 279 10 123 5.49e-18 81.6
MS.gene004518.t1 AT4G12850 42.574 101 48 1 28 118 10 110 2.50e-18 84.0
MS.gene004518.t1 AT4G12850 38.732 142 61 4 179 300 10 145 5.03e-18 83.2
MS.gene004518.t1 AT4G12850 40.909 110 52 2 19 118 4 110 2.92e-18 82.4
MS.gene004518.t1 AT4G12850 44.000 100 46 1 179 268 10 109 1.47e-17 80.5
MS.gene004518.t1 AT2G43280 39.815 108 51 1 24 117 17 124 4.96e-18 84.0
MS.gene004518.t1 AT2G43280 36.364 132 70 2 178 295 20 151 3.45e-16 78.6
MS.gene004518.t1 AT2G43280 33.835 133 74 1 44 162 2 134 2.37e-14 72.0
MS.gene004518.t1 AT2G43280 36.522 115 59 2 195 295 2 116 1.91e-13 69.7

Find 152 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAATTGCATGAGCATATTT+TGG 0.147366 2.4:-66083249 MS.gene004518:CDS
CTGGGTTCCGTTCTATTTAA+AGG 0.153193 2.4:-66082729 MS.gene004518:CDS
TTCTTTGGTGGAAGTTTAAA+TGG 0.183946 2.4:-66082662 MS.gene004518:CDS
TGGTGAATGGCGACATATTT+AGG 0.203007 2.4:-66083408 MS.gene004518:CDS
AGGGTTCAGTTAAATGATTC+TGG 0.241282 2.4:-66084003 MS.gene004518:CDS
CATGCAGCATACTTGGGTTT+TGG 0.249061 2.4:-66083691 MS.gene004518:CDS
CACAAGCAACCTGTGCTTCT+TGG 0.254421 2.4:-66083109 MS.gene004518:CDS
GGCAATAGCAAAAGTCTTTC+CGG 0.278519 2.4:-66082963 MS.gene004518:CDS
TTGCAGTGCTTTAGTTATCT+TGG 0.278930 2.4:-66082452 MS.gene004518:CDS
CCATGCTCATGCAGCATACT+TGG 0.293439 2.4:-66083698 MS.gene004518:CDS
CTTTATCGATCGAGAACTAA+TGG 0.295969 2.4:-66084105 MS.gene004518:CDS
GATTCCATGCTTTCTTTCAT+AGG 0.296754 2.4:+66082686 None:intergenic
GTAGAAGACTCGATAGCTTT+TGG 0.308596 2.4:+66083898 None:intergenic
ATAACACCAAAGGGAGAAAT+AGG 0.311248 2.4:+66083823 None:intergenic
GAAAGCATGGAATCATTCTT+TGG 0.319532 2.4:-66082677 MS.gene004518:CDS
GGTGAACCTATTTCTCCCTT+TGG 0.320761 2.4:-66083829 MS.gene004518:CDS
TATGCCATGGAAGTTGATAA+TGG 0.354230 2.4:-66083271 MS.gene004518:CDS
TGGGTTCCGTTCTATTTAAA+GGG 0.358637 2.4:-66082728 MS.gene004518:CDS
CTGCATGAGCATGGTAAAAT+TGG 0.364754 2.4:+66083707 None:intergenic
TAAAATTGGTAGGCTTCATT+AGG 0.371960 2.4:+66083721 None:intergenic
ATCGAATGGGATTTAAAGTT+CGG 0.379216 2.4:-66084137 MS.gene004518:CDS
AAAAGATCATAATCATGATC+TGG 0.383971 2.4:-66083503 MS.gene004518:CDS
ATATAGAAAGAGTGTTTATA+TGG 0.389131 2.4:-66083164 MS.gene004518:CDS
CTGGTTGTCCAGCATTCATA+AGG 0.391197 2.4:-66084023 MS.gene004518:CDS
AGTGCTGTACTCGTCAAATA+TGG 0.393973 2.4:-66082800 MS.gene004518:CDS
CGCTTTGTGTGCTCAAAAGA+AGG 0.394545 2.4:-66083613 MS.gene004518:CDS
AGGGTCCCTCAGCAGATATT+TGG 0.395130 2.4:-66082411 MS.gene004518:CDS
CATAATCATGATCTGGGCCT+TGG 0.409497 2.4:-66083496 MS.gene004518:CDS
CCACAACGAAAAGCGATGAT+TGG 0.412913 2.4:+66082937 None:intergenic
TCGCCGGTCTGATGGGTAAT+GGG 0.413317 2.4:-66082889 MS.gene004518:CDS
GCCTTACAGCCAACTCTTGA+AGG 0.417533 2.4:+66083580 None:intergenic
CGTCAAATTCATCGACTGTC+TGG 0.424860 2.4:+66082831 None:intergenic
CTTCTGCACTGTTCTTCTAC+TGG 0.426641 2.4:+66082518 None:intergenic
CGAGGAGGTAAACACTGAAT+TGG 0.426954 2.4:-66083428 MS.gene004518:CDS
TGGTAATGAGTAATGCAATT+GGG 0.428684 2.4:-66084251 MS.gene004518:CDS
CCCTTCAAGAGTTGGCTGTA+AGG 0.428884 2.4:-66083581 MS.gene004518:CDS
AGATACTCATGTAGTCAGTT+TGG 0.430685 2.4:-66083211 MS.gene004518:CDS
CTCGCCGGTCTGATGGGTAA+TGG 0.438659 2.4:-66082890 MS.gene004518:CDS
AACTGAACCCTTATGAATGC+TGG 0.443026 2.4:+66084015 None:intergenic
TTCATTCCGCGTTATGAAAG+AGG 0.443774 2.4:-66082620 MS.gene004518:CDS
TGTCTAGCTCCTCTTAGGAC+AGG 0.444148 2.4:+66083373 None:intergenic
GCTACAACTAATGTCAAGAT+TGG 0.453950 2.4:+66082334 None:intergenic
TTGTCTTACGTTCCGTTCCA+AGG 0.458529 2.4:+66083479 None:intergenic
CTGGTAATGAGTAATGCAAT+TGG 0.460733 2.4:-66084252 MS.gene004518:CDS
ATATCTTATGGTAATCGAAT+GGG 0.462953 2.4:-66084150 MS.gene004518:CDS
GAAAAGCGATGATTGGTTAC+CGG 0.465829 2.4:+66082944 None:intergenic
TTCTTTGTAGACACGTGTTG+AGG 0.466046 2.4:-66082286 MS.gene004518:CDS
TAAGTGTGGAAATGATGATA+TGG 0.473912 2.4:-66082384 MS.gene004518:CDS
AAAAGAAGAACTTGAAGTAC+AGG 0.474189 2.4:-66083782 MS.gene004518:CDS
AGGATCATGGAGTAGCTCTC+AGG 0.475535 2.4:-66082171 MS.gene004518:CDS
TCAAGGCAAGCAGAAGATAC+AGG 0.476103 2.4:-66083301 MS.gene004518:CDS
GGATTTAAAATCTATGAAGA+AGG 0.480432 2.4:-66082431 MS.gene004518:CDS
GAGTAATGCAATTGGGGAGG+AGG 0.480640 2.4:-66084244 MS.gene004518:CDS
GCATCCTGTTGGTACTGAAC+TGG 0.481228 2.4:-66084271 MS.gene004518:CDS
TTTATCGATCGAGAACTAAT+GGG 0.481588 2.4:-66084104 MS.gene004518:CDS
AAAATCACATTAGAAGTGAT+TGG 0.484311 2.4:-66083351 MS.gene004518:CDS
AGCCAACTCTTGAAGGGTGC+TGG 0.486059 2.4:+66083587 None:intergenic
AGGATTAAGAATCAACCATC+AGG 0.487093 2.4:-66083550 MS.gene004518:CDS
TCACTTGGTTGTTTCAGACA+TGG 0.489216 2.4:-66083051 MS.gene004518:CDS
ACCCTCTCGCAAAATCTCAT+AGG 0.490493 2.4:+66082058 None:intergenic
ACCAACATATGGTTCGATCC+TGG 0.490539 2.4:+66083759 None:intergenic
CTGAACTCTTGACCAACATA+TGG 0.491150 2.4:+66083748 None:intergenic
GTTAAATGATTCTGGAAAGT+GGG 0.491161 2.4:-66083995 MS.gene004518:CDS
ATTCATCGACTGTCTGGCTC+TGG 0.491387 2.4:+66082837 None:intergenic
CATCAGCTCAGTTCAAGAAC+TGG 0.495226 2.4:-66084042 MS.gene004518:CDS
AGGAAGAAAATGCTTGAAGT+AGG 0.495697 2.4:-66083856 MS.gene004518:CDS
CAAGAGCTCGCCGGTCTGAT+GGG 0.495940 2.4:-66082896 MS.gene004518:CDS
TGAACTCTGTGAGAGGTGTT+TGG 0.496070 2.4:+66082639 None:intergenic
AGAATCAACCATCAGGAAAG+TGG 0.496214 2.4:-66083543 MS.gene004518:CDS
CAATTCAGTGTTTACCTCCT+CGG 0.500177 2.4:+66083429 None:intergenic
ATGGATTGAAGGACAATGCT+TGG 0.502036 2.4:-66082781 MS.gene004518:CDS
CACTTGGTTGTTTCAGACAT+GGG 0.502099 2.4:-66083050 MS.gene004518:CDS
AAAGATCATAATCATGATCT+GGG 0.502626 2.4:-66083502 MS.gene004518:CDS
TTCCAGCACCCTTCAAGAGT+TGG 0.503277 2.4:-66083589 MS.gene004518:CDS
AGTTAAATGATTCTGGAAAG+TGG 0.505016 2.4:-66083996 MS.gene004518:CDS
ATTCCAACAAAAGTAGCAAA+TGG 0.506439 2.4:+66083139 None:intergenic
ACTCGTCAAATATGGATTGA+AGG 0.507225 2.4:-66082792 MS.gene004518:CDS
CTACTCCATGATCCTATGTC+AGG 0.507285 2.4:+66082179 None:intergenic
ACAAGAGCTCGCCGGTCTGA+TGG 0.507295 2.4:-66082897 MS.gene004518:CDS
TATATCTTATGGTAATCGAA+TGG 0.509718 2.4:-66084151 MS.gene004518:CDS
ATTACCAGTTCAGTACCAAC+AGG 0.512740 2.4:+66084267 None:intergenic
ATCAAGATGTTTCAATCCAG+AGG 0.519473 2.4:-66082985 MS.gene004518:CDS
GATAACTACCCTGTCCTAAG+AGG 0.520837 2.4:-66083382 MS.gene004518:CDS
CATGCTCATGCAGCATACTT+GGG 0.521303 2.4:-66083697 MS.gene004518:CDS
GTATGAGAAGCGTGCATCCT+GGG 0.521503 2.4:-66082747 MS.gene004518:CDS
ATGGGATTTAAAGTTCGGAT+TGG 0.522097 2.4:-66084132 MS.gene004518:CDS
GTTGTGGCAAATTAATGCAA+AGG 0.522725 2.4:-66082921 MS.gene004518:CDS
CCTCAGCATTTCTTGTCCAT+CGG 0.524241 2.4:+66082213 None:intergenic
TACCTCCTCGGTCAAAATGT+TGG 0.526158 2.4:+66083441 None:intergenic
TTGGCTGTAAGGCTTATTTG+AGG 0.528311 2.4:-66083570 MS.gene004518:CDS
AGATTTGTGTGTTCGAAGGA+GGG 0.530744 2.4:-66084066 MS.gene004518:CDS
ATTACCATTATCAACTTCCA+TGG 0.531538 2.4:+66083267 None:intergenic
TCTTTGTAGACACGTGTTGA+GGG 0.535253 2.4:-66082285 MS.gene004518:CDS
TTGAAATTAATAACACCAAA+GGG 0.535464 2.4:+66083814 None:intergenic
TTTCTTGTGATCCATACATA+GGG 0.536566 2.4:-66084212 MS.gene004518:CDS
TGTATGAGAAGCGTGCATCC+TGG 0.538953 2.4:-66082748 MS.gene004518:CDS
ATTTGGGATAATGATTGTAA+AGG 0.539019 2.4:-66084295 None:intergenic
TCGGTCAAAATGTTGGAAGC+AGG 0.543963 2.4:+66083448 None:intergenic
TAGAAGATTTGTGTGTTCGA+AGG 0.548021 2.4:-66084070 MS.gene004518:CDS
TCGTGAAGAAGAAAGAAAAG+AGG 0.548083 2.4:-66082585 MS.gene004518:CDS
AAGATTTGTGTGTTCGAAGG+AGG 0.548897 2.4:-66084067 MS.gene004518:CDS
GGAAGAAAATGCTTGAAGTA+GGG 0.553026 2.4:-66083855 MS.gene004518:CDS
GCATCTAAATACATAGATGC+TGG 0.554139 2.4:-66082110 MS.gene004518:CDS
TCGACTACCCACTTTCCTGA+TGG 0.556567 2.4:+66083535 None:intergenic
TGGTTGTCCAGCATTCATAA+GGG 0.557324 2.4:-66084022 MS.gene004518:CDS
GTAAACACTGAATTGGTGAA+TGG 0.557824 2.4:-66083421 MS.gene004518:CDS
GAAGCACAGGTTGCTTGTGG+TGG 0.561382 2.4:+66083113 None:intergenic
GTCTAGCTCCTCTTAGGACA+GGG 0.562105 2.4:+66083374 None:intergenic
TCATGATCTGGGCCTTGGAA+CGG 0.573122 2.4:-66083491 MS.gene004518:CDS
GATTTAAAATCTATGAAGAA+GGG 0.574074 2.4:-66082430 MS.gene004518:CDS
CCAAGTATGCTGCATGAGCA+TGG 0.574108 2.4:+66083698 None:intergenic
AATTCCTATGAAAGAAAGCA+TGG 0.575352 2.4:-66082690 MS.gene004518:CDS
GGGTAAGAGCAATGTCAGGC+CGG 0.576033 2.4:-66083030 MS.gene004518:CDS
AGATATTTGGTGCGTAAGTG+TGG 0.576360 2.4:-66082398 MS.gene004518:CDS
AATCTGAAGAATCTTTCACT+TGG 0.577117 2.4:-66083066 MS.gene004518:CDS
ACATGGGTAAGAGCAATGTC+AGG 0.578816 2.4:-66083034 MS.gene004518:CDS
GAATCAACCATCAGGAAAGT+GGG 0.580534 2.4:-66083542 MS.gene004518:CDS
AGAGCACATCCAAGAAGCAC+AGG 0.580657 2.4:+66083100 None:intergenic
TCGATGAATTTGACGCCTCG+TGG 0.582578 2.4:-66082823 MS.gene004518:CDS
AAGAACTTGAAGTACAGGCC+AGG 0.583073 2.4:-66083777 MS.gene004518:CDS
GGAGAAAACTGTGTTGGCAC+AGG 0.583735 2.4:-66082034 MS.gene004518:CDS
TGTCAGATGTTTGAATATGA+AGG 0.583906 2.4:-66082311 MS.gene004518:CDS
CCTTACAGCCAACTCTTGAA+GGG 0.585947 2.4:+66083581 None:intergenic
GCTTTGTGTGCTCAAAAGAA+GGG 0.587200 2.4:-66083612 MS.gene004518:CDS
CTCGCTACATCTTACACCGA+TGG 0.590796 2.4:-66082229 MS.gene004518:CDS
CAAGAAGCACAGGTTGCTTG+TGG 0.592747 2.4:+66083110 None:intergenic
ATTGTAAAGGAGCATCCTGT+TGG 0.597584 2.4:-66084282 MS.gene004518:CDS
GCCAGGATCGAACCATATGT+TGG 0.601674 2.4:-66083760 MS.gene004518:CDS
AATGTGCCCTTTAAATAGAA+CGG 0.602152 2.4:+66082722 None:intergenic
CTTTAAATAGAACGGAACCC+AGG 0.602743 2.4:+66082730 None:intergenic
CTGAAGTCACTCGCAAGCCA+AGG 0.602865 2.4:-66083876 MS.gene004518:CDS
GGTAATGAGTAATGCAATTG+GGG 0.604332 2.4:-66084250 MS.gene004518:CDS
ATGAGCATGGTAAAATTGGT+AGG 0.604922 2.4:+66083711 None:intergenic
AGCATGGAATCATTCTTTGG+TGG 0.604989 2.4:-66082674 MS.gene004518:CDS
GCAAAGGAACAAGAGCTCGC+CGG 0.611525 2.4:-66082905 MS.gene004518:CDS
AAAATGCTTGAAGTAGGGAA+TGG 0.612218 2.4:-66083850 MS.gene004518:CDS
TGGACAAGAAATGCTGAGGA+TGG 0.619508 2.4:-66082209 MS.gene004518:CDS
CTTGAAATTAATAACACCAA+AGG 0.620334 2.4:+66083813 None:intergenic
TACGCACCAAATATCTGCTG+AGG 0.623181 2.4:+66082405 None:intergenic
CTATTACTGACAAAGGCTGC+CGG 0.623252 2.4:+66083011 None:intergenic
CGCGGAATGAACTCTGTGAG+AGG 0.627384 2.4:+66082632 None:intergenic
TGATCAGCTATTACTGACAA+AGG 0.628429 2.4:+66083004 None:intergenic
AGGGTGGGAGAAAACTGTGT+TGG 0.629447 2.4:-66082040 MS.gene004518:CDS
GAACCCATTACCCATCAGAC+CGG 0.633308 2.4:+66082886 None:intergenic
CGGTGTAAGATGTAGCGAGA+AGG 0.633470 2.4:+66082233 None:intergenic
ACGCACCAAATATCTGCTGA+GGG 0.642827 2.4:+66082406 None:intergenic
TCAAGATGTTTCAATCCAGA+GGG 0.646236 2.4:-66082984 MS.gene004518:CDS
AATGAGTAATGCAATTGGGG+AGG 0.656176 2.4:-66084247 MS.gene004518:CDS
CCGATGGACAAGAAATGCTG+AGG 0.681471 2.4:-66082213 MS.gene004518:CDS
TCGAGACCTCTTTCATAACG+CGG 0.715495 2.4:+66082614 None:intergenic
GACGAGTACAGCACTCCACG+AGG 0.756116 2.4:+66082808 None:intergenic
TATATGATGTATCTACAACG+AGG 0.828520 2.4:+66083182 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAAGTTTTATATATCTTA+TGG - chr2.4:66082154-66082173 MS.gene004518:CDS 15.0%
!! TTGAAATTAATAACACCAAA+GGG + chr2.4:66082505-66082524 None:intergenic 20.0%
!! TTGCAAAAAAGTTTGAAAAT+TGG + chr2.4:66083769-66083788 None:intergenic 20.0%
!!! ATATAGAAAGAGTGTTTATA+TGG - chr2.4:66083152-66083171 MS.gene004518:CDS 20.0%
!!! GATTTAAAATCTATGAAGAA+GGG - chr2.4:66083886-66083905 MS.gene004518:CDS 20.0%
! AAAAGATCATAATCATGATC+TGG - chr2.4:66082813-66082832 MS.gene004518:CDS 25.0%
! AAAATCACATTAGAAGTGAT+TGG - chr2.4:66082965-66082984 MS.gene004518:CDS 25.0%
! AAAGATCATAATCATGATCT+GGG - chr2.4:66082814-66082833 MS.gene004518:CDS 25.0%
! AGTTAAAAAGTTTGATGCTA+TGG - chr2.4:66084168-66084187 MS.gene004518:CDS 25.0%
! ATATCTTATGGTAATCGAAT+GGG - chr2.4:66082166-66082185 MS.gene004518:CDS 25.0%
! CTTGAAATTAATAACACCAA+AGG + chr2.4:66082506-66082525 None:intergenic 25.0%
! TATATCTTATGGTAATCGAA+TGG - chr2.4:66082165-66082184 MS.gene004518:CDS 25.0%
!! GGATTTAAAATCTATGAAGA+AGG - chr2.4:66083885-66083904 MS.gene004518:CDS 25.0%
!! TAATTGCATGAGCATATTTT+GGG - chr2.4:66083068-66083087 MS.gene004518:CDS 25.0%
!!! GATTATGATCTTTTTCAAGA+CGG + chr2.4:66082807-66082826 None:intergenic 25.0%
!!! TTGTTTCGTTCTAAAAATGA+CGG - chr2.4:66082664-66082683 MS.gene004518:CDS 25.0%
!!! TTTTAGAGTATTTTCAGTCA+AGG - chr2.4:66082998-66083017 MS.gene004518:CDS 25.0%
AAAAACTCTAACCCTATGTA+TGG + chr2.4:66082118-66082137 None:intergenic 30.0%
AAAAGAAGAACTTGAAGTAC+AGG - chr2.4:66082534-66082553 MS.gene004518:CDS 30.0%
AATCTGAAGAATCTTTCACT+TGG - chr2.4:66083250-66083269 MS.gene004518:CDS 30.0%
AATGTGCCCTTTAAATAGAA+CGG + chr2.4:66083597-66083616 None:intergenic 30.0%
AATTCCTATGAAAGAAAGCA+TGG - chr2.4:66083626-66083645 MS.gene004518:CDS 30.0%
ATTACCATTATCAACTTCCA+TGG + chr2.4:66083052-66083071 None:intergenic 30.0%
ATTCCAACAAAAGTAGCAAA+TGG + chr2.4:66083180-66083199 None:intergenic 30.0%
TAAAATTGGTAGGCTTCATT+AGG + chr2.4:66082598-66082617 None:intergenic 30.0%
TAAGTGTGGAAATGATGATA+TGG - chr2.4:66083932-66083951 MS.gene004518:CDS 30.0%
TATATGATGTATCTACAACG+AGG + chr2.4:66083137-66083156 None:intergenic 30.0%
TGTCAGATGTTTGAATATGA+AGG - chr2.4:66084005-66084024 MS.gene004518:CDS 30.0%
TTCTTTGGTGGAAGTTTAAA+TGG - chr2.4:66083654-66083673 MS.gene004518:CDS 30.0%
TTTATCGATCGAGAACTAAT+GGG - chr2.4:66082212-66082231 MS.gene004518:CDS 30.0%
TTTCTTGTGATCCATACATA+GGG - chr2.4:66082104-66082123 MS.gene004518:CDS 30.0%
! AGTTAAATGATTCTGGAAAG+TGG - chr2.4:66082320-66082339 MS.gene004518:CDS 30.0%
! ATCGAATGGGATTTAAAGTT+CGG - chr2.4:66082179-66082198 MS.gene004518:CDS 30.0%
! GGTTGTTGATCATTTTCATA+AGG - chr2.4:66082342-66082361 MS.gene004518:CDS 30.0%
! GTAATTGCATGAGCATATTT+TGG - chr2.4:66083067-66083086 MS.gene004518:CDS 30.0%
! GTTAAATGATTCTGGAAAGT+GGG - chr2.4:66082321-66082340 MS.gene004518:CDS 30.0%
! TGGTAATGAGTAATGCAATT+GGG - chr2.4:66082065-66082084 MS.gene004518:CDS 30.0%
! TTTTCTTGTGATCCATACAT+AGG - chr2.4:66082103-66082122 MS.gene004518:CDS 30.0%
!! GTTGAGGGTTTTTCAAATAT+TGG - chr2.4:66084046-66084065 MS.gene004518:CDS 30.0%
!!! AGGGTTAGAGTTTTTTAATG+CGG - chr2.4:66082123-66082142 MS.gene004518:CDS 30.0%
AAAATGCTTGAAGTAGGGAA+TGG - chr2.4:66082466-66082485 MS.gene004518:CDS 35.0%
AGATACTCATGTAGTCAGTT+TGG - chr2.4:66083105-66083124 MS.gene004518:CDS 35.0%
AGGAAGAAAATGCTTGAAGT+AGG - chr2.4:66082460-66082479 MS.gene004518:CDS 35.0%
AGGATTAAGAATCAACCATC+AGG - chr2.4:66082766-66082785 MS.gene004518:CDS 35.0%
AGGGTTCAGTTAAATGATTC+TGG - chr2.4:66082313-66082332 MS.gene004518:CDS 35.0%
ATAACACCAAAGGGAGAAAT+AGG + chr2.4:66082496-66082515 None:intergenic 35.0%
ATCAAGATGTTTCAATCCAG+AGG - chr2.4:66083331-66083350 MS.gene004518:CDS 35.0%
ATGAGCATGGTAAAATTGGT+AGG + chr2.4:66082608-66082627 None:intergenic 35.0%
CTTTATCGATCGAGAACTAA+TGG - chr2.4:66082211-66082230 MS.gene004518:CDS 35.0%
GATTCCATGCTTTCTTTCAT+AGG + chr2.4:66083633-66083652 None:intergenic 35.0%
GCATCTAAATACATAGATGC+TGG - chr2.4:66084206-66084225 MS.gene004518:CDS 35.0%
GCTACAACTAATGTCAAGAT+TGG + chr2.4:66083985-66084004 None:intergenic 35.0%
GGAAGAAAATGCTTGAAGTA+GGG - chr2.4:66082461-66082480 MS.gene004518:CDS 35.0%
GGTAATGAGTAATGCAATTG+GGG - chr2.4:66082066-66082085 MS.gene004518:CDS 35.0%
GTAAACACTGAATTGGTGAA+TGG - chr2.4:66082895-66082914 MS.gene004518:CDS 35.0%
GTTGTGGCAAATTAATGCAA+AGG - chr2.4:66083395-66083414 MS.gene004518:CDS 35.0%
TAGAAGATTTGTGTGTTCGA+AGG - chr2.4:66082246-66082265 MS.gene004518:CDS 35.0%
TCAAGATGTTTCAATCCAGA+GGG - chr2.4:66083332-66083351 MS.gene004518:CDS 35.0%
TCGTGAAGAAGAAAGAAAAG+AGG - chr2.4:66083731-66083750 MS.gene004518:CDS 35.0%
TGATCAGCTATTACTGACAA+AGG + chr2.4:66083315-66083334 None:intergenic 35.0%
TGGGTTCCGTTCTATTTAAA+GGG - chr2.4:66083588-66083607 MS.gene004518:CDS 35.0%
TTGCAGTGCTTTAGTTATCT+TGG - chr2.4:66083864-66083883 MS.gene004518:CDS 35.0%
! ACTCGTCAAATATGGATTGA+AGG - chr2.4:66083524-66083543 MS.gene004518:CDS 35.0%
! ACTTCAAGCATTTTCTTCCT+TGG + chr2.4:66082460-66082479 None:intergenic 35.0%
! ATGGGATTTAAAGTTCGGAT+TGG - chr2.4:66082184-66082203 MS.gene004518:CDS 35.0%
! CTGGTAATGAGTAATGCAAT+TGG - chr2.4:66082064-66082083 MS.gene004518:CDS 35.0%
! GAAAGCATGGAATCATTCTT+TGG - chr2.4:66083639-66083658 MS.gene004518:CDS 35.0%
! GATGGTATTTTTCCTGACAT+AGG - chr2.4:66084125-66084144 MS.gene004518:CDS 35.0%
! TACAGGATTCTTTTATGCCA+TGG - chr2.4:66083032-66083051 MS.gene004518:CDS 35.0%
! TTTTTCCTGACATAGGATCA+TGG - chr2.4:66084132-66084151 MS.gene004518:CDS 35.0%
!! CACTTTCATTTTCAAGAGTG+TGG + chr2.4:66082375-66082394 None:intergenic 35.0%
!! TATGCCATGGAAGTTGATAA+TGG - chr2.4:66083045-66083064 MS.gene004518:CDS 35.0%
!!! GTACCATTTGCTACTTTTGT+TGG - chr2.4:66083174-66083193 MS.gene004518:CDS 35.0%
AACTGAACCCTTATGAATGC+TGG + chr2.4:66082304-66082323 None:intergenic 40.0%
AAGATTTGTGTGTTCGAAGG+AGG - chr2.4:66082249-66082268 MS.gene004518:CDS 40.0%
AATGAGTAATGCAATTGGGG+AGG - chr2.4:66082069-66082088 MS.gene004518:CDS 40.0%
AGAATCAACCATCAGGAAAG+TGG - chr2.4:66082773-66082792 MS.gene004518:CDS 40.0%
AGATTTGTGTGTTCGAAGGA+GGG - chr2.4:66082250-66082269 MS.gene004518:CDS 40.0%
AGCATGGAATCATTCTTTGG+TGG - chr2.4:66083642-66083661 MS.gene004518:CDS 40.0%
AGTGCTGTACTCGTCAAATA+TGG - chr2.4:66083516-66083535 MS.gene004518:CDS 40.0%
ATTACCAGTTCAGTACCAAC+AGG + chr2.4:66082052-66082071 None:intergenic 40.0%
CAATTCAGTGTTTACCTCCT+CGG + chr2.4:66082890-66082909 None:intergenic 40.0%
CTGAACTCTTGACCAACATA+TGG + chr2.4:66082571-66082590 None:intergenic 40.0%
CTGCATGAGCATGGTAAAAT+TGG + chr2.4:66082612-66082631 None:intergenic 40.0%
CTGGGTTCCGTTCTATTTAA+AGG - chr2.4:66083587-66083606 MS.gene004518:CDS 40.0%
CTTTAAATAGAACGGAACCC+AGG + chr2.4:66083589-66083608 None:intergenic 40.0%
GAATCAACCATCAGGAAAGT+GGG - chr2.4:66082774-66082793 MS.gene004518:CDS 40.0%
GCTTTGTGTGCTCAAAAGAA+GGG - chr2.4:66082704-66082723 MS.gene004518:CDS 40.0%
GGCAATAGCAAAAGTCTTTC+CGG - chr2.4:66083353-66083372 MS.gene004518:CDS 40.0%
TCTTTGTAGACACGTGTTGA+GGG - chr2.4:66084031-66084050 MS.gene004518:CDS 40.0%
TGGTGAATGGCGACATATTT+AGG - chr2.4:66082908-66082927 MS.gene004518:CDS 40.0%
TGGTTGTCCAGCATTCATAA+GGG - chr2.4:66082294-66082313 MS.gene004518:CDS 40.0%
TTCATTCCGCGTTATGAAAG+AGG - chr2.4:66083696-66083715 MS.gene004518:CDS 40.0%
TTCTTTGTAGACACGTGTTG+AGG - chr2.4:66084030-66084049 MS.gene004518:CDS 40.0%
TTGGCTGTAAGGCTTATTTG+AGG - chr2.4:66082746-66082765 MS.gene004518:CDS 40.0%
! ATGGATTGAAGGACAATGCT+TGG - chr2.4:66083535-66083554 MS.gene004518:CDS 40.0%
! CACTTGGTTGTTTCAGACAT+GGG - chr2.4:66083266-66083285 MS.gene004518:CDS 40.0%
! GAAAAGCGATGATTGGTTAC+CGG + chr2.4:66083375-66083394 None:intergenic 40.0%
! GTAGAAGACTCGATAGCTTT+TGG + chr2.4:66082421-66082440 None:intergenic 40.0%
! TCACTTGGTTGTTTCAGACA+TGG - chr2.4:66083265-66083284 MS.gene004518:CDS 40.0%
!! AGATATTTGGTGCGTAAGTG+TGG - chr2.4:66083918-66083937 MS.gene004518:CDS 40.0%
!! ATAGCTTTTGGCTGCAATGT+TGG + chr2.4:66082409-66082428 None:intergenic 40.0%
!! ATGAGCATATTTTGGGCTGA+CGG - chr2.4:66083075-66083094 MS.gene004518:CDS 40.0%
!! ATTGTAAAGGAGCATCCTGT+TGG - chr2.4:66082034-66082053 MS.gene004518:CDS 40.0%
!! GATTTTGTCTAGCTCCTCTT+AGG + chr2.4:66082951-66082970 None:intergenic 40.0%
!! TTAAAGGGCACATTTTCTGC+TGG - chr2.4:66083603-66083622 MS.gene004518:CDS 40.0%
!!! TTGGGTTTTGGAATTCGAGT+CGG - chr2.4:66082637-66082656 MS.gene004518:CDS 40.0%
AAGAACTTGAAGTACAGGCC+AGG - chr2.4:66082539-66082558 MS.gene004518:CDS 45.0%
ACATGGGTAAGAGCAATGTC+AGG - chr2.4:66083282-66083301 MS.gene004518:CDS 45.0%
ACCAACATATGGTTCGATCC+TGG + chr2.4:66082560-66082579 None:intergenic 45.0%
ACCCTCTCGCAAAATCTCAT+AGG + chr2.4:66084261-66084280 None:intergenic 45.0%
ACGCACCAAATATCTGCTGA+GGG + chr2.4:66083913-66083932 None:intergenic 45.0%
CATAATCATGATCTGGGCCT+TGG - chr2.4:66082820-66082839 MS.gene004518:CDS 45.0%
CATCAGCTCAGTTCAAGAAC+TGG - chr2.4:66082274-66082293 MS.gene004518:CDS 45.0%
CATGCTCATGCAGCATACTT+GGG - chr2.4:66082619-66082638 MS.gene004518:CDS 45.0%
CCACAACGAAAAGCGATGAT+TGG + chr2.4:66083382-66083401 None:intergenic 45.0%
CCTCAGCATTTCTTGTCCAT+CGG + chr2.4:66084106-66084125 None:intergenic 45.0%
CCTTACAGCCAACTCTTGAA+GGG + chr2.4:66082738-66082757 None:intergenic 45.0%
CGAGGAGGTAAACACTGAAT+TGG - chr2.4:66082888-66082907 MS.gene004518:CDS 45.0%
CGCTTTGTGTGCTCAAAAGA+AGG - chr2.4:66082703-66082722 MS.gene004518:CDS 45.0%
CTACTCCATGATCCTATGTC+AGG + chr2.4:66084140-66084159 None:intergenic 45.0%
CTATTACTGACAAAGGCTGC+CGG + chr2.4:66083308-66083327 None:intergenic 45.0%
CTGGTTGTCCAGCATTCATA+AGG - chr2.4:66082293-66082312 MS.gene004518:CDS 45.0%
CTTCTGCACTGTTCTTCTAC+TGG + chr2.4:66083801-66083820 None:intergenic 45.0%
GATAACTACCCTGTCCTAAG+AGG - chr2.4:66082934-66082953 MS.gene004518:CDS 45.0%
TACCTCCTCGGTCAAAATGT+TGG + chr2.4:66082878-66082897 None:intergenic 45.0%
TACGCACCAAATATCTGCTG+AGG + chr2.4:66083914-66083933 None:intergenic 45.0%
TCAAGGCAAGCAGAAGATAC+AGG - chr2.4:66083015-66083034 MS.gene004518:CDS 45.0%
TCGAGACCTCTTTCATAACG+CGG + chr2.4:66083705-66083724 None:intergenic 45.0%
TCGGTCAAAATGTTGGAAGC+AGG + chr2.4:66082871-66082890 None:intergenic 45.0%
TGGACAAGAAATGCTGAGGA+TGG - chr2.4:66084107-66084126 MS.gene004518:CDS 45.0%
TTGTCTTACGTTCCGTTCCA+AGG + chr2.4:66082840-66082859 None:intergenic 45.0%
! ATGAGATTTTGCGAGAGGGT+GGG - chr2.4:66084261-66084280 MS.gene004518:CDS 45.0%
! CATGCAGCATACTTGGGTTT+TGG - chr2.4:66082625-66082644 MS.gene004518:CDS 45.0%
! CGTCAAATTCATCGACTGTC+TGG + chr2.4:66083488-66083507 None:intergenic 45.0%
! GCCTATGAGATTTTGCGAGA+GGG - chr2.4:66084257-66084276 MS.gene004518:CDS 45.0%
! GGTGAACCTATTTCTCCCTT+TGG - chr2.4:66082487-66082506 MS.gene004518:CDS 45.0%
! TATGAGATTTTGCGAGAGGG+TGG - chr2.4:66084260-66084279 MS.gene004518:CDS 45.0%
! TGCCTATGAGATTTTGCGAG+AGG - chr2.4:66084256-66084275 MS.gene004518:CDS 45.0%
!! CCAATCATCGCTTTTCGTTG+TGG - chr2.4:66083379-66083398 MS.gene004518:CDS 45.0%
!! TGAACTCTGTGAGAGGTGTT+TGG + chr2.4:66083680-66083699 None:intergenic 45.0%
!! TGCTTCCAACATTTTGACCG+AGG - chr2.4:66082870-66082889 MS.gene004518:CDS 45.0%
!! TTCCAACATTTTGACCGAGG+AGG - chr2.4:66082873-66082892 MS.gene004518:CDS 45.0%
!!! AGACTTTTGCTATTGCCCTC+TGG + chr2.4:66083350-66083369 None:intergenic 45.0%
AGGATCATGGAGTAGCTCTC+AGG - chr2.4:66084145-66084164 MS.gene004518:CDS 50.0%
AGGGTCCCTCAGCAGATATT+TGG - chr2.4:66083905-66083924 MS.gene004518:CDS 50.0%
AGGGTGGGAGAAAACTGTGT+TGG - chr2.4:66084276-66084295 MS.gene004518:CDS 50.0%
CACAAGCAACCTGTGCTTCT+TGG - chr2.4:66083207-66083226 MS.gene004518:CDS 50.0%
CCAAGTATGCTGCATGAGCA+TGG + chr2.4:66082621-66082640 None:intergenic 50.0%
CCATGCTCATGCAGCATACT+TGG - chr2.4:66082618-66082637 MS.gene004518:CDS 50.0%
CCCTTCAAGAGTTGGCTGTA+AGG - chr2.4:66082735-66082754 MS.gene004518:CDS 50.0%
CCGATGGACAAGAAATGCTG+AGG - chr2.4:66084103-66084122 MS.gene004518:CDS 50.0%
CGGTGTAAGATGTAGCGAGA+AGG + chr2.4:66084086-66084105 None:intergenic 50.0%
CTCGCTACATCTTACACCGA+TGG - chr2.4:66084087-66084106 MS.gene004518:CDS 50.0%
GAACCCATTACCCATCAGAC+CGG + chr2.4:66083433-66083452 None:intergenic 50.0%
GAGTAATGCAATTGGGGAGG+AGG - chr2.4:66082072-66082091 MS.gene004518:CDS 50.0%
GCCAGGATCGAACCATATGT+TGG - chr2.4:66082556-66082575 MS.gene004518:CDS 50.0%
GCCTTACAGCCAACTCTTGA+AGG + chr2.4:66082739-66082758 None:intergenic 50.0%
TCATGATCTGGGCCTTGGAA+CGG - chr2.4:66082825-66082844 MS.gene004518:CDS 50.0%
TCGACTACCCACTTTCCTGA+TGG + chr2.4:66082784-66082803 None:intergenic 50.0%
TCGATGAATTTGACGCCTCG+TGG - chr2.4:66083493-66083512 MS.gene004518:CDS 50.0%
TTCCAGCACCCTTCAAGAGT+TGG - chr2.4:66082727-66082746 MS.gene004518:CDS 50.0%
! AGAGCACATCCAAGAAGCAC+AGG + chr2.4:66083219-66083238 None:intergenic 50.0%
! ATTCATCGACTGTCTGGCTC+TGG + chr2.4:66083482-66083501 None:intergenic 50.0%
! CAAGAAGCACAGGTTGCTTG+TGG + chr2.4:66083209-66083228 None:intergenic 50.0%
! GCATCCTGTTGGTACTGAAC+TGG - chr2.4:66082045-66082064 MS.gene004518:CDS 50.0%
! GGAGAAAACTGTGTTGGCAC+AGG - chr2.4:66084282-66084301 MS.gene004518:CDS 50.0%
! GTATGAGAAGCGTGCATCCT+GGG - chr2.4:66083569-66083588 MS.gene004518:CDS 50.0%
! GTCTAGCTCCTCTTAGGACA+GGG + chr2.4:66082945-66082964 None:intergenic 50.0%
! TGTATGAGAAGCGTGCATCC+TGG - chr2.4:66083568-66083587 MS.gene004518:CDS 50.0%
! TGTCTAGCTCCTCTTAGGAC+AGG + chr2.4:66082946-66082965 None:intergenic 50.0%
CGCGGAATGAACTCTGTGAG+AGG + chr2.4:66083687-66083706 None:intergenic 55.0%
CTGAAGTCACTCGCAAGCCA+AGG - chr2.4:66082440-66082459 MS.gene004518:CDS 55.0%
GAAGCACAGGTTGCTTGTGG+TGG + chr2.4:66083206-66083225 None:intergenic 55.0%
GCAAAGGAACAAGAGCTCGC+CGG - chr2.4:66083411-66083430 MS.gene004518:CDS 55.0%
GGGTAAGAGCAATGTCAGGC+CGG - chr2.4:66083286-66083305 MS.gene004518:CDS 55.0%
! AGCCAACTCTTGAAGGGTGC+TGG + chr2.4:66082732-66082751 None:intergenic 55.0%
! TCGCCGGTCTGATGGGTAAT+GGG - chr2.4:66083427-66083446 MS.gene004518:CDS 55.0%
ACAAGAGCTCGCCGGTCTGA+TGG - chr2.4:66083419-66083438 MS.gene004518:CDS 60.0%
CAAGAGCTCGCCGGTCTGAT+GGG - chr2.4:66083420-66083439 MS.gene004518:CDS 60.0%
GACGAGTACAGCACTCCACG+AGG + chr2.4:66083511-66083530 None:intergenic 60.0%
! CTCGCCGGTCTGATGGGTAA+TGG - chr2.4:66083426-66083445 MS.gene004518:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 66082031 66084307 66082031 ID=MS.gene004518
chr2.4 mRNA 66082031 66084307 66082031 ID=MS.gene004518.t1;Parent=MS.gene004518
chr2.4 exon 66082031 66084307 66082031 ID=MS.gene004518.t1.exon1;Parent=MS.gene004518.t1
chr2.4 CDS 66082031 66084307 66082031 ID=cds.MS.gene004518.t1;Parent=MS.gene004518.t1
Gene Sequence

>MS.gene004518

ATGATTGTAAAGGAGCATCCTGTTGGTACTGAACTGGTAATGAGTAATGCAATTGGGGAGGAGGAAATTGATTTTTCTTGTGATCCATACATAGGGTTAGAGTTTTTTAATGCGGATGACGCGCTTAAGTTTTATATATCTTATGGTAATCGAATGGGATTTAAAGTTCGGATTGGTCAACTTTATCGATCGAGAACTAATGGGTCTGTTTCTTCTAGAAGATTTGTGTGTTCGAAGGAGGGACATCAGCTCAGTTCAAGAACTGGTTGTCCAGCATTCATAAGGGTTCAGTTAAATGATTCTGGAAAGTGGGTTGTTGATCATTTTCATAAGGATCATAACCACACTCTTGAAAATGAAAGTGAAAGTTTCGCTCCAACATTGCAGCCAAAAGCTATCGAGTCTTCTACTGAAGTCACTCGCAAGCCAAGGAAGAAAATGCTTGAAGTAGGGAATGGTGAACCTATTTCTCCCTTTGGTGTTATTAATTTCAAGCGTATGAGAAAAGAAGAACTTGAAGTACAGGCCAGGATCGAACCATATGTTGGTCAAGAGTTCAGTACTCCTAATGAAGCCTACCAATTTTACCATGCTCATGCAGCATACTTGGGTTTTGGAATTCGAGTCGGTCAATTGTTTCGTTCTAAAAATGACGGATCAATTACATCTCGACGCTTTGTGTGCTCAAAAGAAGGGTTCCAGCACCCTTCAAGAGTTGGCTGTAAGGCTTATTTGAGGATTAAGAATCAACCATCAGGAAAGTGGGTAGTCGACCGTCTTGAAAAAGATCATAATCATGATCTGGGCCTTGGAACGGAACGTAAGACAAAAAGTCTTCCTGCTTCCAACATTTTGACCGAGGAGGTAAACACTGAATTGGTGAATGGCGACATATTTAGGATAGATAACTACCCTGTCCTAAGAGGAGCTAGACAAAATCACATTAGAAGTGATTGGTACAACATGCTTTTAGAGTATTTTCAGTCAAGGCAAGCAGAAGATACAGGATTCTTTTATGCCATGGAAGTTGATAATGGTAATTGCATGAGCATATTTTGGGCTGACGGAAGATCTAGATACTCATGTAGTCAGTTTGGCGATGTCCTCGTTGTAGATACATCATATAGAAAGAGTGTTTATATGGTACCATTTGCTACTTTTGTTGGAATTAACCACCACAAGCAACCTGTGCTTCTTGGATGTGCTCTAATTGCTGATGAATCTGAAGAATCTTTCACTTGGTTGTTTCAGACATGGGTAAGAGCAATGTCAGGCCGGCAGCCTTTGTCAGTAATAGCTGATCAAGATGTTTCAATCCAGAGGGCAATAGCAAAAGTCTTTCCGGTAACCAATCATCGCTTTTCGTTGTGGCAAATTAATGCAAAGGAACAAGAGCTCGCCGGTCTGATGGGTAATGGGTTCACTAAAGATTATGAAAAGTGTGTTTACCAGAGCCAGACAGTCGATGAATTTGACGCCTCGTGGAGTGCTGTACTCGTCAAATATGGATTGAAGGACAATGCTTGGCTAAAAGAAATGTATGAGAAGCGTGCATCCTGGGTTCCGTTCTATTTAAAGGGCACATTTTCTGCTGGAATTCCTATGAAAGAAAGCATGGAATCATTCTTTGGTGGAAGTTTAAATGGCCAAACACCTCTCACAGAGTTCATTCCGCGTTATGAAAGAGGTCTCGAGCGACGTCGTGAAGAAGAAAGAAAAGAGGATTTTAATACTTCCAATTTTCAAACTTTTTTGCAAACAAAAGAGCCAGTAGAAGAACAGTGCAGAAGACTTTACACTCTTGCAGTATTCAAAGTATTTCAGAAAGAGCTTTTGCAGTGCTTTAGTTATCTTGGATTTAAAATCTATGAAGAAGGGTCCCTCAGCAGATATTTGGTGCGTAAGTGTGGAAATGATGATATGGTGAAGCATGTAGTTACATTCAATGCATCCAATCTTGACATTAGTTGTAGCTGTCAGATGTTTGAATATGAAGGTGTTCTTTGTAGACACGTGTTGAGGGTTTTTCAAATATTGGAAGTAAGAGAAGTTCCTTCTCGCTACATCTTACACCGATGGACAAGAAATGCTGAGGATGGTATTTTTCCTGACATAGGATCATGGAGTAGCTCTCAGGAGTTAAAAAGTTTGATGCTATGGAGTCTAAGAGAAACAGCATCTAAATACATAGATGCTGGTGCATCATCAATTGAAAAGTATAAACTTGCCTATGAGATTTTGCGAGAGGGTGGGAGAAAACTGTGTTGGCACAGGTGA
Protein sequence

>MS.gene004518.t1

MIVKEHPVGTELVMSNAIGEEEIDFSCDPYIGLEFFNADDALKFYISYGNRMGFKVRIGQLYRSRTNGSVSSRRFVCSKEGHQLSSRTGCPAFIRVQLNDSGKWVVDHFHKDHNHTLENESESFAPTLQPKAIESSTEVTRKPRKKMLEVGNGEPISPFGVINFKRMRKEELEVQARIEPYVGQEFSTPNEAYQFYHAHAAYLGFGIRVGQLFRSKNDGSITSRRFVCSKEGFQHPSRVGCKAYLRIKNQPSGKWVVDRLEKDHNHDLGLGTERKTKSLPASNILTEEVNTELVNGDIFRIDNYPVLRGARQNHIRSDWYNMLLEYFQSRQAEDTGFFYAMEVDNGNCMSIFWADGRSRYSCSQFGDVLVVDTSYRKSVYMVPFATFVGINHHKQPVLLGCALIADESEESFTWLFQTWVRAMSGRQPLSVIADQDVSIQRAIAKVFPVTNHRFSLWQINAKEQELAGLMGNGFTKDYEKCVYQSQTVDEFDASWSAVLVKYGLKDNAWLKEMYEKRASWVPFYLKGTFSAGIPMKESMESFFGGSLNGQTPLTEFIPRYERGLERRREEERKEDFNTSNFQTFLQTKEPVEEQCRRLYTLAVFKVFQKELLQCFSYLGFKIYEEGSLSRYLVRKCGNDDMVKHVVTFNASNLDISCSCQMFEYEGVLCRHVLRVFQILEVREVPSRYILHRWTRNAEDGIFPDIGSWSSSQELKSLMLWSLRETASKYIDAGASSIEKYKLAYEILREGGRKLCWHR