AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004696

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004696.t1 MTR_4g102520 97.265 585 14 2 1 584 1 584 0.0 1188
MS.gene004696.t1 MTR_1g072740 98.318 535 8 1 1 535 1 534 0.0 1088
MS.gene004696.t1 MTR_8g467040 33.226 310 193 4 282 581 107 412 1.86e-51 182
MS.gene004696.t1 MTR_4g119620 27.964 329 210 10 270 584 6 321 2.51e-33 130
MS.gene004696.t1 MTR_1g072140 25.325 308 217 8 281 584 14 312 1.25e-29 119
MS.gene004696.t1 MTR_7g086360 26.730 318 220 9 268 581 32 340 2.60e-26 110
MS.gene004696.t1 MTR_8g092620 27.687 307 203 10 282 584 6 297 1.39e-24 104
MS.gene004696.t1 MTR_8g092620 24.514 257 147 8 283 496 47 299 1.86e-12 68.9
MS.gene004696.t1 MTR_4g104380 25.962 312 206 8 282 584 218 513 3.02e-24 107
MS.gene004696.t1 MTR_4g104380 33.019 106 65 3 273 374 289 392 8.17e-12 68.2
MS.gene004696.t1 MTR_8g092620 27.541 305 202 10 282 582 13 302 3.29e-23 101
MS.gene004696.t1 MTR_3g075400 26.471 306 214 8 281 584 212 508 3.81e-23 103
MS.gene004696.t1 MTR_2g436510 26.299 308 195 12 291 584 167 456 3.96e-19 90.9
MS.gene004696.t1 MTR_7g076230 26.132 287 188 9 292 560 367 647 5.91e-19 91.7
MS.gene004696.t1 MTR_8g092620 28.037 214 139 8 282 491 6 208 1.52e-16 79.7
MS.gene004696.t1 MTR_6g004040 29.825 171 111 6 285 453 92 255 1.60e-16 83.6
MS.gene004696.t1 MTR_6g004040 23.264 288 197 10 290 565 12 287 1.66e-14 77.4
MS.gene004696.t1 MTR_6g004040 24.868 189 135 4 396 584 31 212 3.67e-13 72.8
MS.gene004696.t1 MTR_6g004040 31.538 130 80 4 284 410 133 256 2.44e-11 67.0
MS.gene004696.t1 MTR_1g069345 24.691 324 227 11 267 584 191 503 7.21e-16 80.9
MS.gene004696.t1 MTR_2g012630 29.545 176 114 7 285 458 91 258 1.74e-15 80.5
MS.gene004696.t1 MTR_2g012630 23.737 198 144 4 387 584 21 211 4.10e-13 73.2
MS.gene004696.t1 MTR_2g012630 22.867 293 198 10 287 565 8 286 2.09e-12 70.9
MS.gene004696.t1 MTR_2g012630 29.545 176 114 7 285 458 91 258 2.10e-15 80.5
MS.gene004696.t1 MTR_2g012630 23.737 198 144 4 387 584 21 211 5.32e-13 72.8
MS.gene004696.t1 MTR_2g012630 22.867 293 198 10 287 565 8 286 2.45e-12 70.5
MS.gene004696.t1 MTR_1g054300 23.097 381 203 10 285 584 110 481 2.33e-15 79.3
MS.gene004696.t1 MTR_1g097140 25.329 304 204 14 287 584 73 359 3.13e-15 78.6
MS.gene004696.t1 MTR_5g090420 21.338 314 234 8 276 584 243 548 2.23e-14 76.6
MS.gene004696.t1 MTR_3g052220 23.824 319 217 11 283 584 202 511 2.47e-14 76.3
MS.gene004696.t1 MTR_3g116500 24.127 315 215 10 289 584 60 369 2.19e-13 72.4
MS.gene004696.t1 MTR_3g116500 26.222 225 150 7 373 584 38 259 8.43e-13 69.3
MS.gene004696.t1 MTR_8g106790 23.022 278 183 12 282 539 54 320 1.51e-12 69.3
MS.gene004696.t1 MTR_2g060520 21.429 322 228 8 283 584 115 431 6.28e-12 68.2
MS.gene004696.t1 MTR_4g123597 22.812 320 212 14 285 584 347 651 6.32e-12 69.3
MS.gene004696.t1 MTR_4g105700 21.639 305 212 11 298 584 316 611 1.05e-11 68.6
MS.gene004696.t1 MTR_1g034785 24.339 189 117 5 285 451 109 293 6.15e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004696.t1 AT1G10580 77.436 585 118 4 1 584 1 572 0.0 938
MS.gene004696.t1 AT5G54520 32.915 319 204 3 274 584 140 456 9.56e-55 192
MS.gene004696.t1 AT3G49660 25.321 312 215 9 281 584 11 312 2.19e-29 119
MS.gene004696.t1 AT3G49660 28.767 219 145 6 286 499 105 317 1.91e-25 107
MS.gene004696.t1 AT5G64730 27.508 309 201 11 282 584 6 297 7.41e-27 111
MS.gene004696.t1 AT4G15900 29.967 307 183 12 292 584 174 462 4.77e-26 112
MS.gene004696.t1 AT2G43770 26.460 291 206 7 291 578 50 335 1.43e-25 108
MS.gene004696.t1 AT4G02730 24.759 311 216 10 281 584 30 329 1.62e-24 105
MS.gene004696.t1 AT3G16650 28.990 307 185 13 292 584 168 455 6.19e-22 99.8
MS.gene004696.t1 AT5G50230 24.658 292 196 7 282 564 213 489 8.99e-20 93.2
MS.gene004696.t1 AT5G23430 23.256 301 200 12 287 578 9 287 1.59e-17 87.0
MS.gene004696.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.40e-14 77.8
MS.gene004696.t1 AT5G23430 25.000 192 131 4 396 584 31 212 5.39e-13 72.4
MS.gene004696.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.21e-11 67.0
MS.gene004696.t1 AT5G23430 23.256 301 200 12 287 578 9 287 1.60e-17 87.0
MS.gene004696.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.41e-14 77.8
MS.gene004696.t1 AT5G23430 25.000 192 131 4 396 584 31 212 4.94e-13 72.8
MS.gene004696.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.02e-11 67.0
MS.gene004696.t1 AT5G23430 23.256 301 200 12 287 578 9 287 1.60e-17 87.0
MS.gene004696.t1 AT5G23430 30.000 150 97 5 285 432 92 235 1.41e-14 77.8
MS.gene004696.t1 AT5G23430 25.000 192 131 4 396 584 31 212 4.94e-13 72.8
MS.gene004696.t1 AT5G23430 29.921 127 86 3 284 410 133 256 3.02e-11 67.0
MS.gene004696.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.64e-17 86.7
MS.gene004696.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.64e-17 86.7
MS.gene004696.t1 AT5G08560 23.453 307 222 8 282 584 262 559 1.64e-17 86.7
MS.gene004696.t1 AT5G08390 23.913 276 192 9 287 561 9 267 3.40e-17 85.9
MS.gene004696.t1 AT5G08390 32.000 150 94 5 285 432 92 235 2.02e-15 80.5
MS.gene004696.t1 AT5G08390 25.000 192 131 4 396 584 31 212 3.42e-13 73.2
MS.gene004696.t1 AT5G08390 32.283 127 83 3 284 410 133 256 1.98e-12 70.9
MS.gene004696.t1 AT1G61210 31.429 175 112 6 285 458 91 258 7.60e-17 85.1
MS.gene004696.t1 AT1G61210 25.714 280 182 10 292 567 13 270 8.24e-16 81.6
MS.gene004696.t1 AT1G61210 23.810 189 137 4 396 584 30 211 1.43e-11 68.2
MS.gene004696.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.49e-11 68.2
MS.gene004696.t1 AT1G61210 31.429 175 112 6 285 458 91 258 1.03e-16 84.7
MS.gene004696.t1 AT1G61210 25.714 280 182 10 292 567 13 270 1.42e-15 80.9
MS.gene004696.t1 AT1G61210 23.810 189 137 4 396 584 30 211 1.76e-11 67.8
MS.gene004696.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.86e-11 67.8
MS.gene004696.t1 AT1G61210 31.429 175 112 6 285 458 91 258 1.09e-16 84.7
MS.gene004696.t1 AT1G61210 25.714 280 182 10 292 567 13 270 1.44e-15 80.9
MS.gene004696.t1 AT1G61210 23.810 189 137 4 396 584 30 211 1.62e-11 68.2
MS.gene004696.t1 AT1G61210 31.008 129 82 5 284 410 132 255 1.84e-11 67.8
MS.gene004696.t1 AT1G15440 26.457 223 156 5 292 510 347 565 1.28e-16 84.3
MS.gene004696.t1 AT1G15440 26.457 223 156 5 292 510 387 605 1.77e-16 84.0
MS.gene004696.t1 AT5G43920 24.688 320 218 12 278 584 204 513 2.59e-16 82.4
MS.gene004696.t1 AT1G11160 24.742 291 195 9 287 565 8 286 5.79e-16 82.0
MS.gene004696.t1 AT1G11160 27.684 177 120 6 285 460 91 260 6.54e-15 79.0
MS.gene004696.t1 AT1G11160 25.397 189 134 4 396 584 30 211 1.26e-14 77.8
MS.gene004696.t1 AT1G11160 30.534 131 80 6 284 410 132 255 6.07e-11 66.2
MS.gene004696.t1 AT1G11160 24.742 291 195 9 287 565 8 286 5.89e-16 82.0
MS.gene004696.t1 AT1G11160 27.684 177 120 6 285 460 91 260 6.05e-15 79.0
MS.gene004696.t1 AT1G11160 25.397 189 134 4 396 584 30 211 1.32e-14 77.8
MS.gene004696.t1 AT1G11160 30.534 131 80 6 284 410 132 255 5.87e-11 66.2
MS.gene004696.t1 AT1G11160 24.742 291 195 9 287 565 8 286 6.16e-16 82.0
MS.gene004696.t1 AT1G11160 27.684 177 120 6 285 460 91 260 7.14e-15 78.6
MS.gene004696.t1 AT1G11160 25.397 189 134 4 396 584 30 211 1.23e-14 77.8
MS.gene004696.t1 AT1G11160 30.534 131 80 6 284 410 132 255 6.18e-11 66.2
MS.gene004696.t1 AT1G11160 27.684 177 120 6 285 460 49 218 5.78e-15 79.0
MS.gene004696.t1 AT1G11160 25.749 167 117 4 418 584 10 169 1.33e-12 71.2
MS.gene004696.t1 AT1G11160 24.803 254 168 8 324 565 2 244 1.28e-11 68.2
MS.gene004696.t1 AT1G11160 30.534 131 80 6 284 410 90 213 5.35e-11 66.2
MS.gene004696.t1 AT3G18130 24.907 269 175 11 291 539 60 321 6.24e-14 73.6
MS.gene004696.t1 AT3G18130 24.907 269 184 9 293 553 14 272 1.27e-11 66.6
MS.gene004696.t1 AT5G52820 24.497 298 204 10 291 584 191 471 2.40e-13 73.2
MS.gene004696.t1 AT1G48630 24.907 269 177 11 291 541 60 321 6.47e-13 70.5
MS.gene004696.t1 AT1G48630 24.542 273 188 9 289 553 10 272 6.13e-12 67.8
MS.gene004696.t1 AT2G33340 25.591 254 173 9 285 532 255 498 6.74e-13 71.6
MS.gene004696.t1 AT2G33340 25.591 254 173 9 285 532 255 498 6.74e-13 71.6
MS.gene004696.t1 AT5G16750 25.444 169 118 5 289 452 55 220 8.57e-13 72.0
MS.gene004696.t1 AT2G41500 23.228 254 179 7 335 584 252 493 1.50e-12 70.9
MS.gene004696.t1 AT2G41500 25.472 212 152 6 286 496 331 537 4.88e-12 68.9
MS.gene004696.t1 AT2G41500 23.228 254 179 7 335 584 252 493 1.50e-12 70.9
MS.gene004696.t1 AT2G41500 25.472 212 152 6 286 496 331 537 4.88e-12 68.9
MS.gene004696.t1 AT1G73720 24.451 319 214 12 283 584 201 509 3.73e-12 69.3
MS.gene004696.t1 AT3G21540 23.864 264 174 12 334 584 402 651 8.32e-12 68.9
MS.gene004696.t1 AT3G21540 27.094 203 134 8 303 498 26 221 5.88e-11 66.2
MS.gene004696.t1 AT1G79990 22.460 187 141 3 310 494 72 256 1.61e-11 67.8
MS.gene004696.t1 AT2G33340 27.011 174 117 6 285 453 255 423 1.62e-11 67.4
MS.gene004696.t1 AT1G18080 23.420 269 182 9 291 541 60 322 1.80e-11 66.2
MS.gene004696.t1 AT1G18080 22.695 282 161 7 289 562 10 242 1.97e-11 66.2
MS.gene004696.t1 AT1G79990 22.460 187 141 3 310 494 72 256 1.80e-11 67.8
MS.gene004696.t1 AT5G60940 23.125 320 224 9 282 584 111 425 4.13e-11 65.9
MS.gene004696.t1 AT5G60940 23.125 320 224 9 282 584 19 333 5.74e-11 64.7
MS.gene004696.t1 AT5G67320 23.464 358 202 14 281 575 252 600 6.06e-11 65.9

Find 155 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGGTTTGGAGAGGGTTT+TGG 0.192541 1.1:-54866662 None:intergenic
GTGAGGGTAAGTGTTGGTTC+TGG 0.241552 1.1:+54868105 MS.gene004696:CDS
ATCGAAATCACCGACGTAAT+TGG 0.272574 1.1:-54866902 None:intergenic
AGCATGGTGTCGTCGACTTT+GGG 0.273615 1.1:-54866619 None:intergenic
ACATTCTGTTTATCCTCATC+TGG 0.288775 1.1:-54867669 None:intergenic
TAAATGTATGAGGACTTATA+TGG 0.290113 1.1:+54867488 MS.gene004696:CDS
TTACTCGCCTTAGCATCTTT+TGG 0.297645 1.1:-54867312 None:intergenic
CAAGAGTATGATCAGCATTT+GGG 0.309524 1.1:+54867756 MS.gene004696:CDS
TGGATACTAAGGTTAAGATT+TGG 0.323671 1.1:+54867448 MS.gene004696:CDS
GGCGAAGAGAAGAGTGGTTT+TGG 0.323999 1.1:+54867201 MS.gene004696:CDS
TTGAGTTGGGTCGATGGGTT+TGG 0.324589 1.1:-54866674 None:intergenic
TCCCTCAGGGTATGGGAATA+TGG 0.330247 1.1:+54867843 MS.gene004696:CDS
TTGGCTCGCCGCTCAGAGTT+TGG 0.331077 1.1:+54867944 MS.gene004696:CDS
GGATACTAAGGTTAAGATTT+GGG 0.331103 1.1:+54867449 MS.gene004696:CDS
TGATAAGAATATTAAGTATT+GGG 0.340716 1.1:+54867578 MS.gene004696:CDS
GTTCAGTGGGATATGAATAC+TGG 0.343180 1.1:+54867723 MS.gene004696:CDS
CAGCTCTGGGCCCCGATTCA+AGG 0.351014 1.1:+54866727 MS.gene004696:CDS
TGAGGGTAAGTGTTGGTTCT+GGG 0.355149 1.1:+54868106 MS.gene004696:CDS
GAGCATGGTGTCGTCGACTT+TGG 0.357237 1.1:-54866620 None:intergenic
TTTGATTCTGTCTGCTGGTA+TGG 0.366420 1.1:+54867428 MS.gene004696:CDS
TCAAGAGTATGATCAGCATT+TGG 0.367145 1.1:+54867755 MS.gene004696:CDS
CGTTGTTGTCGTCGGTGTAT+TGG 0.369179 1.1:-54866519 None:intergenic
GCGGCGAGCCAATTGGCATT+GGG 0.372981 1.1:-54867933 None:intergenic
AACAACCATGCCTCAGAAGC+TGG 0.373950 1.1:-54867078 None:intergenic
TGGGGCATTCGAAAGCTGTT+AGG 0.375620 1.1:+54867508 MS.gene004696:CDS
CGAAAGGGGTTTCTGCGATT+AGG 0.375961 1.1:+54867385 MS.gene004696:CDS
TTTGCTGGACATATTGTTGC+TGG 0.377860 1.1:+54868026 MS.gene004696:CDS
GGTGAGATGGGCCTTGAATC+GGG 0.381007 1.1:-54866738 None:intergenic
CGATATAACCTGCCCCGTTT+CGG 0.387388 1.1:-54867604 None:intergenic
TTGTGCTATAAATAAGTATC+TGG 0.390504 1.1:-54867971 None:intergenic
ACCCTACCTACGACCAGCTC+TGG 0.397227 1.1:+54866713 MS.gene004696:CDS
CACTTCACCCCAATGCCAAT+TGG 0.397316 1.1:+54867925 MS.gene004696:CDS
GGGATTATCAAGGTAGGTCT+TGG 0.401179 1.1:+54867280 MS.gene004696:CDS
TTTGGGGTCGGCGACGGTTA+GGG 0.403402 1.1:-54866644 None:intergenic
GTAAGTGTTGGTTCTGGGAT+TGG 0.407813 1.1:+54868111 MS.gene004696:CDS
CTTGAATCGGGGCCCAGAGC+TGG 0.409734 1.1:-54866726 None:intergenic
GCAAAGTAGCGACGTGTGGC+TGG 0.411956 1.1:+54868213 MS.gene004696:CDS
AGCGGCGAGCCAATTGGCAT+TGG 0.418827 1.1:-54867934 None:intergenic
GGGTGAGATGGGCCTTGAAT+CGG 0.419829 1.1:-54866739 None:intergenic
ATGATAAGAATATTAAGTAT+TGG 0.420715 1.1:+54867577 MS.gene004696:CDS
AGAATCAAATGACCCGAATT+CGG 0.425737 1.1:-54867414 None:intergenic
TCTTCTTCTGTTCACGGTGT+GGG 0.426132 1.1:-54866963 None:intergenic
GAACGGGAAGGGAGGAGACG+AGG 0.429345 1.1:-54866589 None:intergenic
AAGTATTGGGATACCGAAAC+GGG 0.432545 1.1:+54867591 MS.gene004696:CDS
ATGACAAGTCCCTCAGGGTA+TGG 0.434880 1.1:+54867835 MS.gene004696:CDS
GAGTCCTTGGGCTGGGAAGA+AGG 0.437077 1.1:+54867113 MS.gene004696:CDS
CTCTTCTTCTGTTCACGGTG+TGG 0.437638 1.1:-54866964 None:intergenic
TCAATACTGGTAAATGTATG+AGG 0.444000 1.1:+54867478 MS.gene004696:CDS
CTTCGACGAAACCGAGTTTG+TGG 0.444127 1.1:-54866798 None:intergenic
GCCCAGAGCTGGTCGTAGGT+AGG 0.447192 1.1:-54866715 None:intergenic
GGGTTAAGCTTCACAACATA+AGG 0.453310 1.1:-54867648 None:intergenic
GGTCATTTGATTCTGTCTGC+TGG 0.457625 1.1:+54867423 MS.gene004696:CDS
AAATGTATGAGGACTTATAT+GGG 0.458158 1.1:+54867489 MS.gene004696:CDS
TGAAGAATAAGAAGAGTCCT+TGG 0.458759 1.1:+54867100 MS.gene004696:CDS
GTCTGCTGGTATGGATACTA+AGG 0.461762 1.1:+54867437 MS.gene004696:CDS
TGGGTCGATGGGTTTGGAGA+GGG 0.461924 1.1:-54866668 None:intergenic
GAGGAGCACGCGAAGAAGAA+AGG 0.463092 1.1:+54867180 MS.gene004696:CDS
TGGTTGCGAGTGGCATCCTC+TGG 0.463609 1.1:+54868184 MS.gene004696:CDS
GGGCGGCGGAACGGGAAGGG+AGG 0.465040 1.1:-54866597 None:intergenic
GGCATTGGGGTGAAGTGAAA+TGG 0.465903 1.1:-54867919 None:intergenic
TAGCGACGTGTGGCTGGGAC+GGG 0.469608 1.1:+54868219 MS.gene004696:CDS
AGGTGTTGAGTTGGGTCGAT+GGG 0.471106 1.1:-54866679 None:intergenic
CCCAGAGCTGGTCGTAGGTA+GGG 0.477469 1.1:-54866714 None:intergenic
GTCTAGATCGTTGTTGTCGT+CGG 0.478814 1.1:-54866527 None:intergenic
CTGATATACTTTATAACTAC+AGG 0.479452 1.1:-54867870 None:intergenic
TGTTGCTGGATATGCTTGTC+AGG 0.489525 1.1:+54868040 MS.gene004696:CDS
CCAGTGATGACAAGTCCCTC+AGG 0.489956 1.1:+54867829 MS.gene004696:CDS
AGGCGATAAGGTGTTGAGTT+GGG 0.492099 1.1:-54866687 None:intergenic
GGGGCATTCGAAAGCTGTTA+GGG 0.493324 1.1:+54867509 MS.gene004696:CDS
CATTCTGTTTATCCTCATCT+GGG 0.502022 1.1:-54867668 None:intergenic
CCCTACCTACGACCAGCTCT+GGG 0.505645 1.1:+54866714 MS.gene004696:CDS
CGAAGAGATTGGTACATACA+TGG 0.505690 1.1:+54867352 MS.gene004696:CDS
GCTAAACCGTCTTTAGCGTA+AGG 0.513178 1.1:-54866760 None:intergenic
ACTCTGAGCGGCGAGCCAAT+TGG 0.513719 1.1:-54867940 None:intergenic
TAAGTATTGGGATACCGAAA+CGG 0.518461 1.1:+54867590 MS.gene004696:CDS
CATGTATGTACCAATCTCTT+CGG 0.518553 1.1:-54867351 None:intergenic
ATCTCAAAACCCTAATTCAC+CGG 0.519645 1.1:+54866555 MS.gene004696:CDS
CAAAGTAGCGACGTGTGGCT+GGG 0.520964 1.1:+54868214 MS.gene004696:CDS
AGGTAGGGTTGAAGGCGATA+AGG 0.521290 1.1:-54866699 None:intergenic
TTGGGATACCGAAACGGGGC+AGG 0.523429 1.1:+54867596 MS.gene004696:CDS
GTAGCGACGTGTGGCTGGGA+CGG 0.525013 1.1:+54868218 MS.gene004696:CDS
GTTATATCGACTTTCTCCAC+TGG 0.528482 1.1:+54867618 MS.gene004696:CDS
TCTTTAGCGTAAGGGTGAGA+TGG 0.528553 1.1:-54866751 None:intergenic
GATTGAAAACCCAGCTTCTG+AGG 0.529050 1.1:+54867068 MS.gene004696:CDS
CTGGTCGTAGGTAGGGTTGA+AGG 0.530035 1.1:-54866707 None:intergenic
AAGGCGATAAGGTGTTGAGT+TGG 0.533837 1.1:-54866688 None:intergenic
CTGTTATATACCGAAGAGAT+TGG 0.537473 1.1:+54867341 MS.gene004696:CDS
CCTGAGGGACTTGTCATCAC+TGG 0.537996 1.1:-54867829 None:intergenic
TGATAAGAAGATTGTTCAGT+GGG 0.538924 1.1:+54867710 MS.gene004696:CDS
ATAAGAAGAGTCCTTGGGCT+GGG 0.539663 1.1:+54867106 MS.gene004696:CDS
ACAACCATGCCTCAGAAGCT+GGG 0.540280 1.1:-54867077 None:intergenic
CTTTAGCGTAAGGGTGAGAT+GGG 0.540886 1.1:-54866750 None:intergenic
TGTCACGAAGGCGTTACCAT+TGG 0.543297 1.1:+54868164 MS.gene004696:CDS
AAAACCCAGCTTCTGAGGCA+TGG 0.546170 1.1:+54867073 MS.gene004696:CDS
TTGGGTCGATGGGTTTGGAG+AGG 0.547307 1.1:-54866669 None:intergenic
CATGGAGTGGACATACGAAA+GGG 0.549842 1.1:+54867370 MS.gene004696:CDS
TGACAAGTCCCTCAGGGTAT+GGG 0.550285 1.1:+54867836 MS.gene004696:CDS
CCTTCCTTCTTCCCAGCCCA+AGG 0.553576 1.1:-54867117 None:intergenic
AAGAAAGGCGAAGAGAAGAG+TGG 0.555351 1.1:+54867195 MS.gene004696:CDS
AAGGTGTTGAGTTGGGTCGA+TGG 0.555828 1.1:-54866680 None:intergenic
TGCTGTTCATCGAAGAGGAA+AGG 0.559395 1.1:-54866835 None:intergenic
TGCGCCTCCAAAAGATGCTA+AGG 0.561249 1.1:+54867305 MS.gene004696:CDS
GGGACGGGTTGATAAAGTAC+TGG 0.561357 1.1:+54868234 MS.gene004696:CDS
GAAGAATAAGAAGAGTCCTT+GGG 0.563353 1.1:+54867101 MS.gene004696:CDS
CCTTGGGCTGGGAAGAAGGA+AGG 0.564772 1.1:+54867117 MS.gene004696:CDS
GCATGGTGTCGTCGACTTTG+GGG 0.565100 1.1:-54866618 None:intergenic
GGGAAGAAGGAAGGTTTACA+AGG 0.566983 1.1:+54867126 MS.gene004696:CDS
AATAAGAAGAGTCCTTGGGC+TGG 0.567302 1.1:+54867105 MS.gene004696:CDS
GACATGACAAAACGTCCATC+CGG 0.567963 1.1:-54868074 None:intergenic
TGTCATGTCGGGTGATGGTG+AGG 0.571408 1.1:+54868088 MS.gene004696:CDS
ATTGTTATCGACAAAAGTAA+TGG 0.571823 1.1:-54867790 None:intergenic
AAAGAGGAAAGGGATTATCA+AGG 0.572402 1.1:+54867270 MS.gene004696:CDS
CGGGAAGGGAGGAGACGAGG+TGG 0.573173 1.1:-54866586 None:intergenic
GTGAGATGGGCCTTGAATCG+GGG 0.578748 1.1:-54866737 None:intergenic
CGGCGAGCCAATTGGCATTG+GGG 0.587352 1.1:-54867932 None:intergenic
GGCGACGGTTAGGGCGAGCA+TGG 0.589400 1.1:-54866635 None:intergenic
GACAACAACGAAGATGAAGA+TGG 0.592971 1.1:+54867039 MS.gene004696:CDS
AAGAGTATGATCAGCATTTG+GGG 0.593826 1.1:+54867757 MS.gene004696:CDS
TTCAGTGGGATATGAATACT+GGG 0.594900 1.1:+54867724 MS.gene004696:CDS
TTATATCGACTTTCTCCACT+GGG 0.596005 1.1:+54867619 MS.gene004696:CDS
GCTACTTTGCTCTGTTCCAG+AGG 0.598337 1.1:-54868200 None:intergenic
AGATACTTATTTATAGCACA+AGG 0.600630 1.1:+54867973 MS.gene004696:CDS
GCTAAAGACGGTTTAGCACA+AGG 0.608053 1.1:+54866766 MS.gene004696:CDS
GCGTTACCATTGGTTGCGAG+TGG 0.608524 1.1:+54868174 MS.gene004696:CDS
AGGAAAGGGATTATCAAGGT+AGG 0.608842 1.1:+54867274 MS.gene004696:CDS
CTAAACCGTCTTTAGCGTAA+GGG 0.610141 1.1:-54866759 None:intergenic
ACATGGAGTGGACATACGAA+AGG 0.611220 1.1:+54867369 MS.gene004696:CDS
GTCATGTCGGGTGATGGTGA+GGG 0.625539 1.1:+54868089 MS.gene004696:CDS
CGGGGCCCAGAGCTGGTCGT+AGG 0.625795 1.1:-54866719 None:intergenic
ACCATATTCCCATACCCTGA+GGG 0.627603 1.1:-54867844 None:intergenic
CATGGTGTCGTCGACTTTGG+GGG 0.627690 1.1:-54866617 None:intergenic
CAGTGATGACAAGTCCCTCA+GGG 0.630201 1.1:+54867830 MS.gene004696:CDS
TCTCACCCTTACGCTAAAGA+CGG 0.631080 1.1:+54866754 MS.gene004696:CDS
AGGATGCCACTCGCAACCAA+TGG 0.631424 1.1:-54868180 None:intergenic
TGTTATGCTGTTCATCGAAG+AGG 0.631441 1.1:-54866840 None:intergenic
TTGGGGTGAAGTGAAATGGA+AGG 0.631463 1.1:-54867915 None:intergenic
AGATTGGTACATACATGGAG+TGG 0.644932 1.1:+54867357 MS.gene004696:CDS
CAGAGCAAAGTAGCGACGTG+TGG 0.656241 1.1:+54868209 MS.gene004696:CDS
GTGATAAGAAGATTGTTCAG+TGG 0.659340 1.1:+54867709 MS.gene004696:CDS
CCGACGTAATTGGAAGCAGA+CGG 0.664084 1.1:-54866892 None:intergenic
AATGTATGAGGACTTATATG+GGG 0.667045 1.1:+54867490 MS.gene004696:CDS
TGTGAAGCTTAACCCAGATG+AGG 0.669296 1.1:+54867656 MS.gene004696:CDS
GATACTTATTTATAGCACAA+GGG 0.672388 1.1:+54867974 MS.gene004696:CDS
GGTATGCGTAACCACAAACT+CGG 0.673398 1.1:+54866787 MS.gene004696:CDS
GTGATGGTGAGGGTAAGTGT+TGG 0.677988 1.1:+54868099 MS.gene004696:CDS
CTGGTTATCCAAACTCTGAG+CGG 0.682803 1.1:-54867952 None:intergenic
CCGTCTGCTTCCAATTACGT+CGG 0.687927 1.1:+54866892 MS.gene004696:CDS
ATGGAGTGGACATACGAAAG+GGG 0.691126 1.1:+54867371 MS.gene004696:CDS
AGAACTCTCAAGTGTCACGA+AGG 0.693137 1.1:+54868152 MS.gene004696:CDS
TACCATATTCCCATACCCTG+AGG 0.706671 1.1:-54867845 None:intergenic
GAAGGCATAGAATGCATGTG+TGG 0.724483 1.1:-54867897 None:intergenic
AACATAAGGAATCTTCCCAG+TGG 0.726027 1.1:-54867634 None:intergenic
AGATCAGAAGAAATACGCTG+AGG 0.737161 1.1:+54867161 MS.gene004696:CDS
AGTATTGGGATACCGAAACG+GGG 0.752897 1.1:+54867592 MS.gene004696:CDS
AGAGTATGATCAGCATTTGG+GGG 0.760214 1.1:+54867758 MS.gene004696:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAAGAATATTAAGTAT+TGG + chr1.1:54867577-54867596 MS.gene004696:CDS 15.0%
!! TGATAAGAATATTAAGTATT+GGG + chr1.1:54867578-54867597 MS.gene004696:CDS 15.0%
!!! CATAACACCTTTTTAAAATT+CGG + chr1.1:54866856-54866875 MS.gene004696:CDS 20.0%
! AAATGTATGAGGACTTATAT+GGG + chr1.1:54867489-54867508 MS.gene004696:CDS 25.0%
! AGATACTTATTTATAGCACA+AGG + chr1.1:54867973-54867992 MS.gene004696:CDS 25.0%
! ATTGTTATCGACAAAAGTAA+TGG - chr1.1:54867793-54867812 None:intergenic 25.0%
! CTGATATACTTTATAACTAC+AGG - chr1.1:54867873-54867892 None:intergenic 25.0%
! GATACTTATTTATAGCACAA+GGG + chr1.1:54867974-54867993 MS.gene004696:CDS 25.0%
! TAAATGTATGAGGACTTATA+TGG + chr1.1:54867488-54867507 MS.gene004696:CDS 25.0%
! TTCAACTTAACAAAAAGAAG+AGG + chr1.1:54868003-54868022 MS.gene004696:CDS 25.0%
! TTGTGCTATAAATAAGTATC+TGG - chr1.1:54867974-54867993 None:intergenic 25.0%
!!! AAAGATAAGAGTACTTTTCA+TGG + chr1.1:54867246-54867265 MS.gene004696:CDS 25.0%
!!! AAGATAAGAGTACTTTTCAT+GGG + chr1.1:54867247-54867266 MS.gene004696:CDS 25.0%
!!! AGCATAACCGAATTTTAAAA+AGG - chr1.1:54866866-54866885 None:intergenic 25.0%
!!! GATATTTGTTTTACCAATGA+TGG + chr1.1:54867531-54867550 MS.gene004696:CDS 25.0%
!!! TTACTTTTGTCGATAACAAT+CGG + chr1.1:54867793-54867812 MS.gene004696:CDS 25.0%
AATGTATGAGGACTTATATG+GGG + chr1.1:54867490-54867509 MS.gene004696:CDS 30.0%
CAAAAACTTAGTTCCATCAT+TGG - chr1.1:54867547-54867566 None:intergenic 30.0%
GGATACTAAGGTTAAGATTT+GGG + chr1.1:54867449-54867468 MS.gene004696:CDS 30.0%
TCAATACTGGTAAATGTATG+AGG + chr1.1:54867478-54867497 MS.gene004696:CDS 30.0%
TGATAAGAAGATTGTTCAGT+GGG + chr1.1:54867710-54867729 MS.gene004696:CDS 30.0%
TGGATACTAAGGTTAAGATT+TGG + chr1.1:54867448-54867467 MS.gene004696:CDS 30.0%
! GGATAAACAGAATGTTTTGT+TGG + chr1.1:54867677-54867696 MS.gene004696:CDS 30.0%
! TTTTTCTCTTCTTCTGTTCA+CGG - chr1.1:54866972-54866991 None:intergenic 30.0%
!! ATTTGGGATGTTTTCAATAC+TGG + chr1.1:54867465-54867484 MS.gene004696:CDS 30.0%
!!! ATTAGGTTTTTTCCGAATTC+GGG + chr1.1:54867402-54867421 MS.gene004696:CDS 30.0%
!!! GATTAGGTTTTTTCCGAATT+CGG + chr1.1:54867401-54867420 MS.gene004696:CDS 30.0%
AAAGAGGAAAGGGATTATCA+AGG + chr1.1:54867270-54867289 MS.gene004696:CDS 35.0%
AACAAAAAGAAGAGGTTTGC+TGG + chr1.1:54868011-54868030 MS.gene004696:CDS 35.0%
ACATTCTGTTTATCCTCATC+TGG - chr1.1:54867672-54867691 None:intergenic 35.0%
AGAATCAAATGACCCGAATT+CGG - chr1.1:54867417-54867436 None:intergenic 35.0%
ATCTCAAAACCCTAATTCAC+CGG + chr1.1:54866555-54866574 MS.gene004696:CDS 35.0%
CATGTATGTACCAATCTCTT+CGG - chr1.1:54867354-54867373 None:intergenic 35.0%
CATTCTGTTTATCCTCATCT+GGG - chr1.1:54867671-54867690 None:intergenic 35.0%
CTGTTATATACCGAAGAGAT+TGG + chr1.1:54867341-54867360 MS.gene004696:CDS 35.0%
GAAGAATAAGAAGAGTCCTT+GGG + chr1.1:54867101-54867120 MS.gene004696:CDS 35.0%
GTGATAAGAAGATTGTTCAG+TGG + chr1.1:54867709-54867728 MS.gene004696:CDS 35.0%
TGAAGAATAAGAAGAGTCCT+TGG + chr1.1:54867100-54867119 MS.gene004696:CDS 35.0%
TTCAGTGGGATATGAATACT+GGG + chr1.1:54867724-54867743 MS.gene004696:CDS 35.0%
! AAGAGTATGATCAGCATTTG+GGG + chr1.1:54867757-54867776 MS.gene004696:CDS 35.0%
! CAAGAGTATGATCAGCATTT+GGG + chr1.1:54867756-54867775 MS.gene004696:CDS 35.0%
! GAGAATTTTCCGGTGAATTA+GGG - chr1.1:54866567-54866586 None:intergenic 35.0%
! TCAAGAGTATGATCAGCATT+TGG + chr1.1:54867755-54867774 MS.gene004696:CDS 35.0%
! TTTTCATGGGAAAGAGGAAA+GGG + chr1.1:54867260-54867279 MS.gene004696:CDS 35.0%
!! AAACAGAATGTTTTGTTGGC+TGG + chr1.1:54867681-54867700 MS.gene004696:CDS 35.0%
!! TAAGTATTGGGATACCGAAA+CGG + chr1.1:54867590-54867609 MS.gene004696:CDS 35.0%
!! TTATATCGACTTTCTCCACT+GGG + chr1.1:54867619-54867638 MS.gene004696:CDS 35.0%
!!! TTTTGGTGGTGAAAAAGTTG+AGG + chr1.1:54867218-54867237 MS.gene004696:CDS 35.0%
AACATAAGGAATCTTCCCAG+TGG - chr1.1:54867637-54867656 None:intergenic 40.0%
AGAGTATGATCAGCATTTGG+GGG + chr1.1:54867758-54867777 MS.gene004696:CDS 40.0%
AGATCAGAAGAAATACGCTG+AGG + chr1.1:54867161-54867180 MS.gene004696:CDS 40.0%
AGATTGGTACATACATGGAG+TGG + chr1.1:54867357-54867376 MS.gene004696:CDS 40.0%
AGGAAAGGGATTATCAAGGT+AGG + chr1.1:54867274-54867293 MS.gene004696:CDS 40.0%
ATCGAAATCACCGACGTAAT+TGG - chr1.1:54866905-54866924 None:intergenic 40.0%
CGAAGAGATTGGTACATACA+TGG + chr1.1:54867352-54867371 MS.gene004696:CDS 40.0%
CTAAACCGTCTTTAGCGTAA+GGG - chr1.1:54866762-54866781 None:intergenic 40.0%
GACAACAACGAAGATGAAGA+TGG + chr1.1:54867039-54867058 MS.gene004696:CDS 40.0%
GGGTTAAGCTTCACAACATA+AGG - chr1.1:54867651-54867670 None:intergenic 40.0%
GTTCAGTGGGATATGAATAC+TGG + chr1.1:54867723-54867742 MS.gene004696:CDS 40.0%
TGTTATGCTGTTCATCGAAG+AGG - chr1.1:54866843-54866862 None:intergenic 40.0%
TTTGATTCTGTCTGCTGGTA+TGG + chr1.1:54867428-54867447 MS.gene004696:CDS 40.0%
TTTGCTGGACATATTGTTGC+TGG + chr1.1:54868026-54868045 MS.gene004696:CDS 40.0%
! CTTTTCATGGGAAAGAGGAA+AGG + chr1.1:54867259-54867278 MS.gene004696:CDS 40.0%
! GAGTACTTTTCATGGGAAAG+AGG + chr1.1:54867254-54867273 MS.gene004696:CDS 40.0%
! GGAGAATTTTCCGGTGAATT+AGG - chr1.1:54866568-54866587 None:intergenic 40.0%
! TTACTCGCCTTAGCATCTTT+TGG - chr1.1:54867315-54867334 None:intergenic 40.0%
! TTGTCAGGTCAATTTTTCGC+CGG + chr1.1:54868055-54868074 MS.gene004696:CDS 40.0%
!! AAGTATTGGGATACCGAAAC+GGG + chr1.1:54867591-54867610 MS.gene004696:CDS 40.0%
!! GTTATATCGACTTTCTCCAC+TGG + chr1.1:54867618-54867637 MS.gene004696:CDS 40.0%
!!! GGAACTAAGTTTTTGAGTGC+TGG + chr1.1:54867552-54867571 MS.gene004696:CDS 40.0%
!!! GGTGAATTAGGGTTTTGAGA+TGG - chr1.1:54866556-54866575 None:intergenic 40.0%
AAGAAAGGCGAAGAGAAGAG+TGG + chr1.1:54867195-54867214 MS.gene004696:CDS 45.0%
AATAAGAAGAGTCCTTGGGC+TGG + chr1.1:54867105-54867124 MS.gene004696:CDS 45.0%
ACATGGAGTGGACATACGAA+AGG + chr1.1:54867369-54867388 MS.gene004696:CDS 45.0%
ACCATATTCCCATACCCTGA+GGG - chr1.1:54867847-54867866 None:intergenic 45.0%
AGAACTCTCAAGTGTCACGA+AGG + chr1.1:54868152-54868171 MS.gene004696:CDS 45.0%
ATAAGAAGAGTCCTTGGGCT+GGG + chr1.1:54867106-54867125 MS.gene004696:CDS 45.0%
ATGGAGTGGACATACGAAAG+GGG + chr1.1:54867371-54867390 MS.gene004696:CDS 45.0%
CATGGAGTGGACATACGAAA+GGG + chr1.1:54867370-54867389 MS.gene004696:CDS 45.0%
CTGGTTATCCAAACTCTGAG+CGG - chr1.1:54867955-54867974 None:intergenic 45.0%
CTTTAGCGTAAGGGTGAGAT+GGG - chr1.1:54866753-54866772 None:intergenic 45.0%
GACATGACAAAACGTCCATC+CGG - chr1.1:54868077-54868096 None:intergenic 45.0%
GATTGAAAACCCAGCTTCTG+AGG + chr1.1:54867068-54867087 MS.gene004696:CDS 45.0%
GCTAAACCGTCTTTAGCGTA+AGG - chr1.1:54866763-54866782 None:intergenic 45.0%
GCTAAAGACGGTTTAGCACA+AGG + chr1.1:54866766-54866785 MS.gene004696:CDS 45.0%
GGGAAGAAGGAAGGTTTACA+AGG + chr1.1:54867126-54867145 MS.gene004696:CDS 45.0%
GGGATTATCAAGGTAGGTCT+TGG + chr1.1:54867280-54867299 MS.gene004696:CDS 45.0%
GGTATGCGTAACCACAAACT+CGG + chr1.1:54866787-54866806 MS.gene004696:CDS 45.0%
GGTCATTTGATTCTGTCTGC+TGG + chr1.1:54867423-54867442 MS.gene004696:CDS 45.0%
GTCTAGATCGTTGTTGTCGT+CGG - chr1.1:54866530-54866549 None:intergenic 45.0%
GTCTGCTGGTATGGATACTA+AGG + chr1.1:54867437-54867456 MS.gene004696:CDS 45.0%
TACCATATTCCCATACCCTG+AGG - chr1.1:54867848-54867867 None:intergenic 45.0%
TCTCACCCTTACGCTAAAGA+CGG + chr1.1:54866754-54866773 MS.gene004696:CDS 45.0%
TCTTCTTCTGTTCACGGTGT+GGG - chr1.1:54866966-54866985 None:intergenic 45.0%
TCTTTAGCGTAAGGGTGAGA+TGG - chr1.1:54866754-54866773 None:intergenic 45.0%
TGCTGTTCATCGAAGAGGAA+AGG - chr1.1:54866838-54866857 None:intergenic 45.0%
TGTGAAGCTTAACCCAGATG+AGG + chr1.1:54867656-54867675 MS.gene004696:CDS 45.0%
TGTTGCTGGATATGCTTGTC+AGG + chr1.1:54868040-54868059 MS.gene004696:CDS 45.0%
TTGGGGTGAAGTGAAATGGA+AGG - chr1.1:54867918-54867937 None:intergenic 45.0%
! AGACGAGGTGGAGAATTTTC+CGG - chr1.1:54866577-54866596 None:intergenic 45.0%
! AGTATTGGGATACCGAAACG+GGG + chr1.1:54867592-54867611 MS.gene004696:CDS 45.0%
! ATGGGTTTGGAGAGGGTTTT+GGG - chr1.1:54866664-54866683 None:intergenic 45.0%
! GAAGGCATAGAATGCATGTG+TGG - chr1.1:54867900-54867919 None:intergenic 45.0%
! GATGGACGTTTTGTCATGTC+GGG + chr1.1:54868077-54868096 MS.gene004696:CDS 45.0%
! GGATGGACGTTTTGTCATGT+CGG + chr1.1:54868076-54868095 MS.gene004696:CDS 45.0%
!! AAGGCGATAAGGTGTTGAGT+TGG - chr1.1:54866691-54866710 None:intergenic 45.0%
!! AGGCGATAAGGTGTTGAGTT+GGG - chr1.1:54866690-54866709 None:intergenic 45.0%
!! GTAAGTGTTGGTTCTGGGAT+TGG + chr1.1:54868111-54868130 MS.gene004696:CDS 45.0%
!! TGAGGGTAAGTGTTGGTTCT+GGG + chr1.1:54868106-54868125 MS.gene004696:CDS 45.0%
!!! GAAGAGAAGAGTGGTTTTGG+TGG + chr1.1:54867204-54867223 MS.gene004696:CDS 45.0%
AACAACCATGCCTCAGAAGC+TGG - chr1.1:54867081-54867100 None:intergenic 50.0%
ACAACCATGCCTCAGAAGCT+GGG - chr1.1:54867080-54867099 None:intergenic 50.0%
AGGTAGGGTTGAAGGCGATA+AGG - chr1.1:54866702-54866721 None:intergenic 50.0%
ATGACAAGTCCCTCAGGGTA+TGG + chr1.1:54867835-54867854 MS.gene004696:CDS 50.0%
CACTTCACCCCAATGCCAAT+TGG + chr1.1:54867925-54867944 MS.gene004696:CDS 50.0%
CAGTGATGACAAGTCCCTCA+GGG + chr1.1:54867830-54867849 MS.gene004696:CDS 50.0%
CCGACGTAATTGGAAGCAGA+CGG - chr1.1:54866895-54866914 None:intergenic 50.0%
CCGTCTGCTTCCAATTACGT+CGG + chr1.1:54866892-54866911 MS.gene004696:CDS 50.0%
CGAAAGGGGTTTCTGCGATT+AGG + chr1.1:54867385-54867404 MS.gene004696:CDS 50.0%
CGATATAACCTGCCCCGTTT+CGG - chr1.1:54867607-54867626 None:intergenic 50.0%
CGTTGTTGTCGTCGGTGTAT+TGG - chr1.1:54866522-54866541 None:intergenic 50.0%
CTCTTCTTCTGTTCACGGTG+TGG - chr1.1:54866967-54866986 None:intergenic 50.0%
CTTCGACGAAACCGAGTTTG+TGG - chr1.1:54866801-54866820 None:intergenic 50.0%
GTATGATCAGCATTTGGGGG+CGG + chr1.1:54867761-54867780 MS.gene004696:CDS 50.0%
TCCCTCAGGGTATGGGAATA+TGG + chr1.1:54867843-54867862 MS.gene004696:CDS 50.0%
TGACAAGTCCCTCAGGGTAT+GGG + chr1.1:54867836-54867855 MS.gene004696:CDS 50.0%
TGCGCCTCCAAAAGATGCTA+AGG + chr1.1:54867305-54867324 MS.gene004696:CDS 50.0%
! CAGGTCAATTTTTCGCCGGA+TGG + chr1.1:54868059-54868078 MS.gene004696:CDS 50.0%
! CGTTTTGTCATGTCGGGTGA+TGG + chr1.1:54868083-54868102 MS.gene004696:CDS 50.0%
! CTCGCCTTAGCATCTTTTGG+AGG - chr1.1:54867312-54867331 None:intergenic 50.0%
! GCTACTTTGCTCTGTTCCAG+AGG - chr1.1:54868203-54868222 None:intergenic 50.0%
! GGCATTGGGGTGAAGTGAAA+TGG - chr1.1:54867922-54867941 None:intergenic 50.0%
! GGCGAAGAGAAGAGTGGTTT+TGG + chr1.1:54867201-54867220 MS.gene004696:CDS 50.0%
! GGGGCATTCGAAAGCTGTTA+GGG + chr1.1:54867509-54867528 MS.gene004696:CDS 50.0%
! GTGATGGTGAGGGTAAGTGT+TGG + chr1.1:54868099-54868118 MS.gene004696:CDS 50.0%
! TGGGGCATTCGAAAGCTGTT+AGG + chr1.1:54867508-54867527 MS.gene004696:CDS 50.0%
! TGGGTTTGGAGAGGGTTTTG+GGG - chr1.1:54866663-54866682 None:intergenic 50.0%
!! AAAACCCAGCTTCTGAGGCA+TGG + chr1.1:54867073-54867092 MS.gene004696:CDS 50.0%
!! AAGGTGTTGAGTTGGGTCGA+TGG - chr1.1:54866683-54866702 None:intergenic 50.0%
!! AGCATGGTGTCGTCGACTTT+GGG - chr1.1:54866622-54866641 None:intergenic 50.0%
!! AGGTGTTGAGTTGGGTCGAT+GGG - chr1.1:54866682-54866701 None:intergenic 50.0%
!! GATGGGTTTGGAGAGGGTTT+TGG - chr1.1:54866665-54866684 None:intergenic 50.0%
!! GGGACGGGTTGATAAAGTAC+TGG + chr1.1:54868234-54868253 MS.gene004696:CDS 50.0%
!! GTGAGGGTAAGTGTTGGTTC+TGG + chr1.1:54868105-54868124 MS.gene004696:CDS 50.0%
!! TGTCACGAAGGCGTTACCAT+TGG + chr1.1:54868164-54868183 MS.gene004696:CDS 50.0%
!! TTGAGTTGGGTCGATGGGTT+TGG - chr1.1:54866677-54866696 None:intergenic 50.0%
!!! TTTGGAGAGGGTTTTGGGGT+CGG - chr1.1:54866659-54866678 None:intergenic 50.0%
AGGATGCCACTCGCAACCAA+TGG - chr1.1:54868183-54868202 None:intergenic 55.0%
CAAAGTAGCGACGTGTGGCT+GGG + chr1.1:54868214-54868233 MS.gene004696:CDS 55.0%
CAGAGCAAAGTAGCGACGTG+TGG + chr1.1:54868209-54868228 MS.gene004696:CDS 55.0%
CCAGTGATGACAAGTCCCTC+AGG + chr1.1:54867829-54867848 MS.gene004696:CDS 55.0%
CCTGAGGGACTTGTCATCAC+TGG - chr1.1:54867832-54867851 None:intergenic 55.0%
CTGGTCGTAGGTAGGGTTGA+AGG - chr1.1:54866710-54866729 None:intergenic 55.0%
GGGTGAGATGGGCCTTGAAT+CGG - chr1.1:54866742-54866761 None:intergenic 55.0%
GGTGAGATGGGCCTTGAATC+GGG - chr1.1:54866741-54866760 None:intergenic 55.0%
GTGAGATGGGCCTTGAATCG+GGG - chr1.1:54866740-54866759 None:intergenic 55.0%
! GAGGAGCACGCGAAGAAGAA+AGG + chr1.1:54867180-54867199 MS.gene004696:CDS 55.0%
! GCGTTACCATTGGTTGCGAG+TGG + chr1.1:54868174-54868193 MS.gene004696:CDS 55.0%
! GTCATGTCGGGTGATGGTGA+GGG + chr1.1:54868089-54868108 MS.gene004696:CDS 55.0%
! TGGGTCGATGGGTTTGGAGA+GGG - chr1.1:54866671-54866690 None:intergenic 55.0%
! TGTCATGTCGGGTGATGGTG+AGG + chr1.1:54868088-54868107 MS.gene004696:CDS 55.0%
! TTGGGTCGATGGGTTTGGAG+AGG - chr1.1:54866672-54866691 None:intergenic 55.0%
!! CATGGTGTCGTCGACTTTGG+GGG - chr1.1:54866620-54866639 None:intergenic 55.0%
!! GAGCATGGTGTCGTCGACTT+TGG - chr1.1:54866623-54866642 None:intergenic 55.0%
!! GCATGGTGTCGTCGACTTTG+GGG - chr1.1:54866621-54866640 None:intergenic 55.0%
ACCCTACCTACGACCAGCTC+TGG + chr1.1:54866713-54866732 MS.gene004696:CDS 60.0%
ACTCTGAGCGGCGAGCCAAT+TGG - chr1.1:54867943-54867962 None:intergenic 60.0%
AGCGGCGAGCCAATTGGCAT+TGG - chr1.1:54867937-54867956 None:intergenic 60.0%
CCCAGAGCTGGTCGTAGGTA+GGG - chr1.1:54866717-54866736 None:intergenic 60.0%
CCCTACCTACGACCAGCTCT+GGG + chr1.1:54866714-54866733 MS.gene004696:CDS 60.0%
CCTTCCTTCTTCCCAGCCCA+AGG - chr1.1:54867120-54867139 None:intergenic 60.0%
CGGCGAGCCAATTGGCATTG+GGG - chr1.1:54867935-54867954 None:intergenic 60.0%
GCAAAGTAGCGACGTGTGGC+TGG + chr1.1:54868213-54868232 MS.gene004696:CDS 60.0%
GCGGCGAGCCAATTGGCATT+GGG - chr1.1:54867936-54867955 None:intergenic 60.0%
TGGTTGCGAGTGGCATCCTC+TGG + chr1.1:54868184-54868203 MS.gene004696:CDS 60.0%
TTGGGATACCGAAACGGGGC+AGG + chr1.1:54867596-54867615 MS.gene004696:CDS 60.0%
! CCTTGGGCTGGGAAGAAGGA+AGG + chr1.1:54867117-54867136 MS.gene004696:CDS 60.0%
! GAGTCCTTGGGCTGGGAAGA+AGG + chr1.1:54867113-54867132 MS.gene004696:CDS 60.0%
! TTGGCTCGCCGCTCAGAGTT+TGG + chr1.1:54867944-54867963 MS.gene004696:CDS 60.0%
! TTTGGGGTCGGCGACGGTTA+GGG - chr1.1:54866647-54866666 None:intergenic 60.0%
!!! TTTTGGGGTCGGCGACGGTT+AGG - chr1.1:54866648-54866667 None:intergenic 60.0%
CTTGAATCGGGGCCCAGAGC+TGG - chr1.1:54866729-54866748 None:intergenic 65.0%
GAACGGGAAGGGAGGAGACG+AGG - chr1.1:54866592-54866611 None:intergenic 65.0%
GCCCAGAGCTGGTCGTAGGT+AGG - chr1.1:54866718-54866737 None:intergenic 65.0%
GTAGCGACGTGTGGCTGGGA+CGG + chr1.1:54868218-54868237 MS.gene004696:CDS 65.0%
TAGCGACGTGTGGCTGGGAC+GGG + chr1.1:54868219-54868238 MS.gene004696:CDS 65.0%
!! CAGCTCTGGGCCCCGATTCA+AGG + chr1.1:54866727-54866746 MS.gene004696:CDS 65.0%
!! GGTGTCGTCGACTTTGGGGG+CGG - chr1.1:54866617-54866636 None:intergenic 65.0%
!!! GAGGGTTTTGGGGTCGGCGA+CGG - chr1.1:54866653-54866672 None:intergenic 65.0%
CGGGAAGGGAGGAGACGAGG+TGG - chr1.1:54866589-54866608 None:intergenic 70.0%
GGCGACGGTTAGGGCGAGCA+TGG - chr1.1:54866638-54866657 None:intergenic 70.0%
! CGACTTTGGGGGCGGCGGAA+CGG - chr1.1:54866609-54866628 None:intergenic 70.0%
! GACTTTGGGGGCGGCGGAAC+GGG - chr1.1:54866608-54866627 None:intergenic 70.0%
!! GTCGTCGACTTTGGGGGCGG+CGG - chr1.1:54866614-54866633 None:intergenic 70.0%
CGGGGCCCAGAGCTGGTCGT+AGG - chr1.1:54866722-54866741 None:intergenic 75.0%
TGGGGGCGGCGGAACGGGAA+GGG - chr1.1:54866603-54866622 None:intergenic 75.0%
TTGGGGGCGGCGGAACGGGA+AGG - chr1.1:54866604-54866623 None:intergenic 75.0%
! GGGCGGCGGAACGGGAAGGG+AGG - chr1.1:54866600-54866619 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 54866505 54868259 54866505 ID=MS.gene004696
chr1.1 mRNA 54866505 54868259 54866505 ID=MS.gene004696.t1;Parent=MS.gene004696
chr1.1 exon 54866505 54868259 54866505 ID=MS.gene004696.t1.exon1;Parent=MS.gene004696.t1
chr1.1 CDS 54866505 54868259 54866505 ID=cds.MS.gene004696.t1;Parent=MS.gene004696.t1
Gene Sequence

>MS.gene004696

ATGGATCTACTCAACCAATACACCGACGACAACAACGATCTAGACTCACCATCTCAAAACCCTAATTCACCGGAAAATTCTCCACCTCGTCTCCTCCCTTCCCGTTCCGCCGCCCCCAAAGTCGACGACACCATGCTCGCCCTAACCGTCGCCGACCCCAAAACCCTCTCCAAACCCATCGACCCAACTCAACACCTTATCGCCTTCAACCCTACCTACGACCAGCTCTGGGCCCCGATTCAAGGCCCATCTCACCCTTACGCTAAAGACGGTTTAGCACAAGGTATGCGTAACCACAAACTCGGTTTCGTCGAAGACGCTAATATCGAACCTTTCCTCTTCGATGAACAGCATAACACCTTTTTAAAATTCGGTTATGCTGCTGATCCGTCTGCTTCCAATTACGTCGGTGATTTCGATGCTTTGCAGAAGAATAATGCTGCTTCTGTTTATAATATCCCACACCGTGAACAGAAGAAGAGAAAAATCGAATCTCTTAAGAAGAAAGAAGACAATGATAACGAAGAAGACAATGACAACAACGAAGATGAAGATGGTGTTGAGATTGAAAACCCAGCTTCTGAGGCATGGTTGTTGAAGAATAAGAAGAGTCCTTGGGCTGGGAAGAAGGAAGGTTTACAAGGAGAGTTAACTGAAGATCAGAAGAAATACGCTGAGGAGCACGCGAAGAAGAAAGGCGAAGAGAAGAGTGGTTTTGGTGGTGAAAAAGTTGAGGCTGTTAAAGATAAGAGTACTTTTCATGGGAAAGAGGAAAGGGATTATCAAGGTAGGTCTTGGATTGCGCCTCCAAAAGATGCTAAGGCGAGTAATGATCACTGTTATATACCGAAGAGATTGGTACATACATGGAGTGGACATACGAAAGGGGTTTCTGCGATTAGGTTTTTTCCGAATTCGGGTCATTTGATTCTGTCTGCTGGTATGGATACTAAGGTTAAGATTTGGGATGTTTTCAATACTGGTAAATGTATGAGGACTTATATGGGGCATTCGAAAGCTGTTAGGGATATTTGTTTTACCAATGATGGAACTAAGTTTTTGAGTGCTGGTTATGATAAGAATATTAAGTATTGGGATACCGAAACGGGGCAGGTTATATCGACTTTCTCCACTGGGAAGATTCCTTATGTTGTGAAGCTTAACCCAGATGAGGATAAACAGAATGTTTTGTTGGCTGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATACTGGGCAGATCACTCAAGAGTATGATCAGCATTTGGGGGCGGTGAATACCATTACTTTTGTCGATAACAATCGGAGATTTGTTACTTCCAGTGATGACAAGTCCCTCAGGGTATGGGAATATGGTATTCCTGTAGTTATAAAGTATATCAGTGAGCCACACATGCATTCTATGCCTTCCATTTCACTTCACCCCAATGCCAATTGGCTCGCCGCTCAGAGTTTGGATAACCAGATACTTATTTATAGCACAAGGGAGAAGTTTCAACTTAACAAAAAGAAGAGGTTTGCTGGACATATTGTTGCTGGATATGCTTGTCAGGTCAATTTTTCGCCGGATGGACGTTTTGTCATGTCGGGTGATGGTGAGGGTAAGTGTTGGTTCTGGGATTGGAAGAGTTGCAAAGTCTTTAGAACTCTCAAGTGTCACGAAGGCGTTACCATTGGTTGCGAGTGGCATCCTCTGGAACAGAGCAAAGTAGCGACGTGTGGCTGGGACGGGTTGATAAAGTACTGGTAA
Protein sequence

>MS.gene004696.t1

MDLLNQYTDDNNDLDSPSQNPNSPENSPPRLLPSRSAAPKVDDTMLALTVADPKTLSKPIDPTQHLIAFNPTYDQLWAPIQGPSHPYAKDGLAQGMRNHKLGFVEDANIEPFLFDEQHNTFLKFGYAADPSASNYVGDFDALQKNNAASVYNIPHREQKKRKIESLKKKEDNDNEEDNDNNEDEDGVEIENPASEAWLLKNKKSPWAGKKEGLQGELTEDQKKYAEEHAKKKGEEKSGFGGEKVEAVKDKSTFHGKEERDYQGRSWIAPPKDAKASNDHCYIPKRLVHTWSGHTKGVSAIRFFPNSGHLILSAGMDTKVKIWDVFNTGKCMRTYMGHSKAVRDICFTNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDEDKQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEYGIPVVIKYISEPHMHSMPSISLHPNANWLAAQSLDNQILIYSTREKFQLNKKKRFAGHIVAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVTIGCEWHPLEQSKVATCGWDGLIKYW