Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005422.t1 | XP_003591303.1 | 98.6 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 1.20E-75 | 292.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005422.t1 | G7IBQ3 | 98.6 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 8.5e-76 | 292.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20912 | MS.gene005422 | PPI |
| MS.gene21134 | MS.gene005422 | PPI |
| MS.gene07368 | MS.gene005422 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005422.t1 | MTR_1g086000 | 98.601 | 143 | 2 | 0 | 1 | 143 | 1 | 143 | 5.54e-104 | 293 |
| MS.gene005422.t1 | MTR_6g060170 | 40.260 | 77 | 46 | 0 | 42 | 118 | 3 | 79 | 2.31e-16 | 70.1 |
| MS.gene005422.t1 | MTR_1g076840 | 32.500 | 80 | 54 | 0 | 39 | 118 | 34 | 113 | 9.06e-13 | 61.6 |
| MS.gene005422.t1 | MTR_6g059920 | 32.258 | 93 | 63 | 0 | 45 | 137 | 36 | 128 | 3.53e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005422.t1 | AT2G32180 | 66.434 | 143 | 43 | 2 | 1 | 142 | 1 | 139 | 8.89e-65 | 194 |
| MS.gene005422.t1 | AT2G32180 | 66.434 | 143 | 43 | 2 | 1 | 142 | 1 | 139 | 8.89e-65 | 194 |
| MS.gene005422.t1 | AT2G32650 | 66.434 | 143 | 43 | 2 | 1 | 142 | 1 | 139 | 9.59e-65 | 194 |
| MS.gene005422.t1 | AT2G32650 | 66.434 | 143 | 43 | 2 | 1 | 142 | 1 | 139 | 9.59e-65 | 194 |
| MS.gene005422.t1 | AT4G10290 | 35.065 | 77 | 47 | 1 | 45 | 118 | 16 | 92 | 2.47e-11 | 57.4 |
| MS.gene005422.t1 | AT4G10300 | 29.703 | 101 | 69 | 1 | 18 | 118 | 17 | 115 | 3.71e-11 | 57.4 |
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTTCATGCAATTTGTTGC+CGG | 0.346254 | 1.1:-63595653 | MS.gene005422:CDS |
| AGTGGTTTGAGGCTGATATT+TGG | 0.378276 | 1.1:-63595610 | MS.gene005422:CDS |
| CTGCCAATCCCATGGCAATC+TGG | 0.379483 | 1.1:+63595736 | None:intergenic |
| ATGTTTCAAGATGGTCAGTA+TGG | 0.381328 | 1.1:-63595772 | MS.gene005422:CDS |
| CGTTATAGTTTCCGAGCATA+TGG | 0.389309 | 1.1:-63595566 | MS.gene005422:CDS |
| ATGAAACGCTTGCTCCCTTC+AGG | 0.397604 | 1.1:+63595668 | None:intergenic |
| CTCCTTGAACCATTGTTGAT+AGG | 0.416620 | 1.1:+63595926 | None:intergenic |
| GAGGCTGATATTTGGTTCAA+TGG | 0.424092 | 1.1:-63595602 | MS.gene005422:CDS |
| ACCATTGTTGATAGGAGAAT+AGG | 0.477458 | 1.1:+63595934 | None:intergenic |
| TTGTTGCCGGAGATTTAATC+CGG | 0.479684 | 1.1:-63595640 | MS.gene005422:CDS |
| CAATGTGAAGGTAGAAAGAA+AGG | 0.481006 | 1.1:-63595822 | MS.gene005422:CDS |
| CCTCAAACCACTTCGGATAC+CGG | 0.486692 | 1.1:+63595621 | None:intergenic |
| AATCCAGATTGCCATGGGAT+TGG | 0.487823 | 1.1:-63595739 | MS.gene005422:CDS |
| CTCCTATCAACAATGGTTCA+AGG | 0.489683 | 1.1:-63595928 | MS.gene005422:CDS |
| CGGATACCGGATTAAATCTC+CGG | 0.495277 | 1.1:+63595634 | None:intergenic |
| CAGATTGCCATGGGATTGGC+AGG | 0.499106 | 1.1:-63595735 | MS.gene005422:CDS |
| GGATTGGCAGGTAGACCAAC+TGG | 0.504664 | 1.1:-63595723 | MS.gene005422:CDS |
| CACATTGTATAATTCTTCCA+AGG | 0.523416 | 1.1:+63595838 | None:intergenic |
| AAACTATAACGTTCTTGATA+CGG | 0.530071 | 1.1:+63595578 | None:intergenic |
| AATGAAGATAGAGAAATCCT+TGG | 0.554986 | 1.1:-63595855 | MS.gene005422:CDS |
| AGATGGTCAGTATGGAAGAC+CGG | 0.563715 | 1.1:-63595764 | MS.gene005422:CDS |
| CTCAGCTTGATGTTTCAAGA+TGG | 0.565626 | 1.1:-63595781 | MS.gene005422:CDS |
| CCGGTATCCGAAGTGGTTTG+AGG | 0.566818 | 1.1:-63595621 | MS.gene005422:CDS |
| AATAATCATCACCATATGCT+CGG | 0.568249 | 1.1:+63595555 | None:intergenic |
| CGGAAAATCCAGATTGCCAT+GGG | 0.572454 | 1.1:-63595744 | MS.gene005422:CDS |
| TCAAGTAACTCTTCTATGCG+AGG | 0.580396 | 1.1:-63595893 | MS.gene005422:CDS |
| CCGGAAAATCCAGATTGCCA+TGG | 0.585607 | 1.1:-63595745 | MS.gene005422:CDS |
| CAAGTAACTCTTCTATGCGA+GGG | 0.589742 | 1.1:-63595892 | MS.gene005422:CDS |
| CTTCTTCTATGTACACCAGT+TGG | 0.594279 | 1.1:+63595708 | None:intergenic |
| ACCTATTCTCCTATCAACAA+TGG | 0.597081 | 1.1:-63595935 | MS.gene005422:CDS |
| CAACTGGTGTACATAGAAGA+AGG | 0.600561 | 1.1:-63595707 | MS.gene005422:CDS |
| AAGTGAGAGTTGTTCCTGAA+GGG | 0.600643 | 1.1:-63595682 | MS.gene005422:CDS |
| GAAGTGAGAGTTGTTCCTGA+AGG | 0.600726 | 1.1:-63595683 | MS.gene005422:CDS |
| TGGTCTACCTGCCAATCCCA+TGG | 0.603266 | 1.1:+63595728 | None:intergenic |
| GGAAGAATTATACAATGTGA+AGG | 0.609686 | 1.1:-63595834 | MS.gene005422:CDS |
| ATTTAATCCGGTATCCGAAG+TGG | 0.624736 | 1.1:-63595628 | MS.gene005422:CDS |
| ATATCAGCCTCAAACCACTT+CGG | 0.644337 | 1.1:+63595614 | None:intergenic |
| GTTGATAGGAGAATAGGTGA+AGG | 0.653020 | 1.1:+63595940 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACTATAACGTTCTTGATA+CGG | + | chr1.1:63595940-63595959 | None:intergenic | 25.0% |
| AATAATCATCACCATATGCT+CGG | + | chr1.1:63595963-63595982 | None:intergenic | 30.0% | |
| AATGAAGATAGAGAAATCCT+TGG | - | chr1.1:63595660-63595679 | MS.gene005422:CDS | 30.0% | |
| CACATTGTATAATTCTTCCA+AGG | + | chr1.1:63595680-63595699 | None:intergenic | 30.0% | |
| GGAAGAATTATACAATGTGA+AGG | - | chr1.1:63595681-63595700 | MS.gene005422:CDS | 30.0% | |
| ACCTATTCTCCTATCAACAA+TGG | - | chr1.1:63595580-63595599 | MS.gene005422:CDS | 35.0% | |
| ATGTTTCAAGATGGTCAGTA+TGG | - | chr1.1:63595743-63595762 | MS.gene005422:CDS | 35.0% | |
| CAATGTGAAGGTAGAAAGAA+AGG | - | chr1.1:63595693-63595712 | MS.gene005422:CDS | 35.0% | |
| ! | ACCATTGTTGATAGGAGAAT+AGG | + | chr1.1:63595584-63595603 | None:intergenic | 35.0% |
| AAGTGAGAGTTGTTCCTGAA+GGG | - | chr1.1:63595833-63595852 | MS.gene005422:CDS | 40.0% | |
| ATATCAGCCTCAAACCACTT+CGG | + | chr1.1:63595904-63595923 | None:intergenic | 40.0% | |
| ATTTAATCCGGTATCCGAAG+TGG | - | chr1.1:63595887-63595906 | MS.gene005422:CDS | 40.0% | |
| CAACTGGTGTACATAGAAGA+AGG | - | chr1.1:63595808-63595827 | MS.gene005422:CDS | 40.0% | |
| CAAGTAACTCTTCTATGCGA+GGG | - | chr1.1:63595623-63595642 | MS.gene005422:CDS | 40.0% | |
| CGTTATAGTTTCCGAGCATA+TGG | - | chr1.1:63595949-63595968 | MS.gene005422:CDS | 40.0% | |
| CTCAGCTTGATGTTTCAAGA+TGG | - | chr1.1:63595734-63595753 | MS.gene005422:CDS | 40.0% | |
| CTCCTATCAACAATGGTTCA+AGG | - | chr1.1:63595587-63595606 | MS.gene005422:CDS | 40.0% | |
| CTCCTTGAACCATTGTTGAT+AGG | + | chr1.1:63595592-63595611 | None:intergenic | 40.0% | |
| CTTCTTCTATGTACACCAGT+TGG | + | chr1.1:63595810-63595829 | None:intergenic | 40.0% | |
| TCAAGTAACTCTTCTATGCG+AGG | - | chr1.1:63595622-63595641 | MS.gene005422:CDS | 40.0% | |
| TTGTTGCCGGAGATTTAATC+CGG | - | chr1.1:63595875-63595894 | MS.gene005422:CDS | 40.0% | |
| ! | AGTGGTTTGAGGCTGATATT+TGG | - | chr1.1:63595905-63595924 | MS.gene005422:CDS | 40.0% |
| ! | GAGGCTGATATTTGGTTCAA+TGG | - | chr1.1:63595913-63595932 | MS.gene005422:CDS | 40.0% |
| ! | GTTGATAGGAGAATAGGTGA+AGG | + | chr1.1:63595578-63595597 | None:intergenic | 40.0% |
| !! | CGTTTCATGCAATTTGTTGC+CGG | - | chr1.1:63595862-63595881 | MS.gene005422:CDS | 40.0% |
| !!! | TCAAGCTGAGCTAGTTTTTC+AGG | + | chr1.1:63595724-63595743 | None:intergenic | 40.0% |
| AATCCAGATTGCCATGGGAT+TGG | - | chr1.1:63595776-63595795 | MS.gene005422:CDS | 45.0% | |
| AGATGGTCAGTATGGAAGAC+CGG | - | chr1.1:63595751-63595770 | MS.gene005422:CDS | 45.0% | |
| CGGAAAATCCAGATTGCCAT+GGG | - | chr1.1:63595771-63595790 | MS.gene005422:CDS | 45.0% | |
| CGGATACCGGATTAAATCTC+CGG | + | chr1.1:63595884-63595903 | None:intergenic | 45.0% | |
| GAAGTGAGAGTTGTTCCTGA+AGG | - | chr1.1:63595832-63595851 | MS.gene005422:CDS | 45.0% | |
| ! | CCATGGCAATCTGGATTTTC+CGG | + | chr1.1:63595773-63595792 | None:intergenic | 45.0% |
| ATGAAACGCTTGCTCCCTTC+AGG | + | chr1.1:63595850-63595869 | None:intergenic | 50.0% | |
| CCGGAAAATCCAGATTGCCA+TGG | - | chr1.1:63595770-63595789 | MS.gene005422:CDS | 50.0% | |
| CCTCAAACCACTTCGGATAC+CGG | + | chr1.1:63595897-63595916 | None:intergenic | 50.0% | |
| CCGGTATCCGAAGTGGTTTG+AGG | - | chr1.1:63595894-63595913 | MS.gene005422:CDS | 55.0% | |
| CTGCCAATCCCATGGCAATC+TGG | + | chr1.1:63595782-63595801 | None:intergenic | 55.0% | |
| TGGTCTACCTGCCAATCCCA+TGG | + | chr1.1:63595790-63595809 | None:intergenic | 55.0% | |
| ! | CAGATTGCCATGGGATTGGC+AGG | - | chr1.1:63595780-63595799 | MS.gene005422:CDS | 55.0% |
| ! | GGATTGGCAGGTAGACCAAC+TGG | - | chr1.1:63595792-63595811 | MS.gene005422:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 63595553 | 63595984 | 63595553 | ID=MS.gene005422 |
| chr1.1 | mRNA | 63595553 | 63595984 | 63595553 | ID=MS.gene005422.t1;Parent=MS.gene005422 |
| chr1.1 | exon | 63595553 | 63595984 | 63595553 | ID=MS.gene005422.t1.exon1;Parent=MS.gene005422.t1 |
| chr1.1 | CDS | 63595553 | 63595984 | 63595553 | ID=cds.MS.gene005422.t1;Parent=MS.gene005422.t1 |
| Gene Sequence |
| Protein sequence |
