AlfalfaGEDB | MS.gene20912

M. sativa cultivar XinJiangDaYe / MS.gene20912

KEGG: -
GO: -
PFAM: PF05899.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20912.t1	XP_003591303.1	99.3	143	1	0	1	143	1	143	6.90E-76	293.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20912.t1	G7IBQ3	99.3	143	1	0	1	143	1	143	5.0e-76	293.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene20912	0.859345	4.41E-63	-1.69E-46
MS.gene049378	MS.gene20912	0.803991	2.68E-49	-1.69E-46
MS.gene049758	MS.gene20912	0.83679	7.61E-57	-1.69E-46
MS.gene04987	MS.gene20912	0.815575	8.62E-52	-1.69E-46
MS.gene050226	MS.gene20912	0.817258	3.62E-52	-1.69E-46
MS.gene050227	MS.gene20912	0.825081	5.69E-54	-1.69E-46
MS.gene050228	MS.gene20912	0.825092	5.66E-54	-1.69E-46
MS.gene050277	MS.gene20912	0.822797	1.95E-53	-1.69E-46
MS.gene050303	MS.gene20912	0.877282	7.12E-69	-1.69E-46
MS.gene050319	MS.gene20912	-0.804616	1.98E-49	-1.69E-46
MS.gene050330	MS.gene20912	-0.838712	2.44E-57	-1.69E-46
MS.gene050438	MS.gene20912	0.800872	1.18E-48	-1.69E-46
MS.gene050558	MS.gene20912	0.830373	3.05E-55	-1.69E-46
MS.gene050623	MS.gene20912	-0.855485	6.11E-62	-1.69E-46
MS.gene050642	MS.gene20912	0.865267	6.68E-65	-1.69E-46
MS.gene050719	MS.gene20912	0.812514	4.08E-51	-1.69E-46
MS.gene050721	MS.gene20912	0.817886	2.61E-52	-1.69E-46
MS.gene050820	MS.gene20912	0.803436	3.49E-49	-1.69E-46
MS.gene051349	MS.gene20912	0.806845	6.75E-50	-1.69E-46
MS.gene051633	MS.gene20912	0.806004	1.02E-49	-1.69E-46
MS.gene051662	MS.gene20912	0.86502	7.99E-65	-1.69E-46
MS.gene051663	MS.gene20912	0.843478	1.37E-58	-1.69E-46
MS.gene051983	MS.gene20912	0.847897	8.64E-60	-1.69E-46
MS.gene052048	MS.gene20912	-0.812874	3.40E-51	-1.69E-46
MS.gene052176	MS.gene20912	0.852636	4.05E-61	-1.69E-46
MS.gene052286	MS.gene20912	0.832416	9.57E-56	-1.69E-46
MS.gene052302	MS.gene20912	0.865705	4.87E-65	-1.69E-46
MS.gene052309	MS.gene20912	-0.870246	1.69E-66	-1.69E-46
MS.gene052358	MS.gene20912	-0.803873	2.83E-49	-1.69E-46
MS.gene052376	MS.gene20912	0.873607	1.29E-67	-1.69E-46
MS.gene052600	MS.gene20912	0.875689	2.53E-68	-1.69E-46
MS.gene052856	MS.gene20912	0.827764	1.31E-54	-1.69E-46
MS.gene052858	MS.gene20912	0.813155	2.95E-51	-1.69E-46
MS.gene052860	MS.gene20912	0.812075	5.09E-51	-1.69E-46
MS.gene053630	MS.gene20912	0.810389	1.18E-50	-1.69E-46
MS.gene053836	MS.gene20912	0.852169	5.51E-61	-1.69E-46
MS.gene053907	MS.gene20912	0.817236	3.66E-52	-1.69E-46
MS.gene054930	MS.gene20912	0.828704	7.75E-55	-1.69E-46
MS.gene054942	MS.gene20912	0.869429	3.12E-66	-1.69E-46
MS.gene054983	MS.gene20912	0.818957	1.49E-52	-1.69E-46
MS.gene055422	MS.gene20912	0.801591	8.37E-49	-1.69E-46
MS.gene056069	MS.gene20912	-0.804538	2.06E-49	-1.69E-46
MS.gene056091	MS.gene20912	0.806258	8.98E-50	-1.69E-46
MS.gene056631	MS.gene20912	0.82609	3.28E-54	-1.69E-46
MS.gene056875	MS.gene20912	0.81177	5.93E-51	-1.69E-46
MS.gene056979	MS.gene20912	0.838004	3.72E-57	-1.69E-46
MS.gene057060	MS.gene20912	0.823299	1.49E-53	-1.69E-46
MS.gene057079	MS.gene20912	0.833783	4.37E-56	-1.69E-46
MS.gene057121	MS.gene20912	0.838139	3.43E-57	-1.69E-46
MS.gene057260	MS.gene20912	0.844298	8.24E-59	-1.69E-46
MS.gene057439	MS.gene20912	0.841689	4.08E-58	-1.69E-46
MS.gene057555	MS.gene20912	0.834306	3.24E-56	-1.69E-46
MS.gene05795	MS.gene20912	0.812007	5.26E-51	-1.69E-46
MS.gene057967	MS.gene20912	0.831703	1.44E-55	-1.69E-46
MS.gene058182	MS.gene20912	0.824201	9.17E-54	-1.69E-46
MS.gene058200	MS.gene20912	0.814663	1.37E-51	-1.69E-46
MS.gene058201	MS.gene20912	0.831445	1.66E-55	-1.69E-46
MS.gene058238	MS.gene20912	0.811219	7.81E-51	-1.69E-46
MS.gene058399	MS.gene20912	0.806361	8.54E-50	-1.69E-46
MS.gene058749	MS.gene20912	0.810924	9.05E-51	-1.69E-46
MS.gene058845	MS.gene20912	0.826672	2.39E-54	-1.69E-46
MS.gene058906	MS.gene20912	0.80819	3.50E-50	-1.69E-46
MS.gene05954	MS.gene20912	0.832432	9.49E-56	-1.69E-46
MS.gene059569	MS.gene20912	0.821234	4.50E-53	-1.69E-46
MS.gene05984	MS.gene20912	0.808044	3.76E-50	-1.69E-46
MS.gene059899	MS.gene20912	0.815238	1.02E-51	-1.69E-46
MS.gene06001	MS.gene20912	0.815718	8.01E-52	-1.69E-46
MS.gene060297	MS.gene20912	0.831089	2.03E-55	-1.69E-46
MS.gene060476	MS.gene20912	0.806427	8.27E-50	-1.69E-46
MS.gene060586	MS.gene20912	0.825078	5.70E-54	-1.69E-46
MS.gene060619	MS.gene20912	0.812329	4.48E-51	-1.69E-46
MS.gene061040	MS.gene20912	-0.827826	1.26E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21134	MS.gene20912	PPI
MS.gene07368	MS.gene20912	PPI
MS.gene20912	MS.gene005422	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20912.t1	MTR_1g086000	99.301	143	1	1	143	1	143	2.68e-104	294
MS.gene20912.t1	MTR_6g060170	40.260	77	46	42	118	3	79	2.42e-16	69.7
MS.gene20912.t1	MTR_1g076840	32.500	80	54	39	118	34	113	8.78e-13	61.6
MS.gene20912.t1	MTR_6g059920	32.258	93	63	45	137	36	128	2.24e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20912.t1	AT2G32650	67.133	143	42	2	1	142	1	139	4.04e-65	195
MS.gene20912.t1	AT2G32650	67.133	143	42	2	1	142	1	139	4.04e-65	195
MS.gene20912.t1	AT2G32180	67.133	143	42	2	1	142	1	139	5.03e-65	194
MS.gene20912.t1	AT2G32180	67.133	143	42	2	1	142	1	139	5.03e-65	194
MS.gene20912.t1	AT4G10290	35.065	77	47	1	45	118	16	92	2.83e-11	57.4
MS.gene20912.t1	AT4G10300	29.703	101	69	1	18	118	17	115	3.95e-11	57.4

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTTCATGCAATTTGTTGC+CGG	0.346254	1.3:-60940510	MS.gene20912:CDS
GAGGCTGATCTTTGGTTCAA+TGG	0.368609	1.3:-60940459	MS.gene20912:CDS
ATGTTTCAAGATGGTCAGTA+TGG	0.381328	1.3:-60940629	MS.gene20912:CDS
AGTGGTTTGAGGCTGATCTT+TGG	0.382114	1.3:-60940467	MS.gene20912:CDS
CGTTATAGTTTCCGAGCATA+TGG	0.389309	1.3:-60940423	MS.gene20912:CDS
ATGAAACGCTTGCTCCCTTC+AGG	0.397604	1.3:+60940525	None:intergenic
CTCCTTGAACCATTGTTGAT+AGG	0.416620	1.3:+60940783	None:intergenic
CTGCCAATCCCATGGCAACC+TGG	0.474702	1.3:+60940593	None:intergenic
ACCATTGTTGATAGGAGAAT+AGG	0.477458	1.3:+60940791	None:intergenic
TTGTTGCCGGAGATTTAATC+CGG	0.479684	1.3:-60940497	MS.gene20912:CDS
CAATGTGAAGGTAGAAAGAA+AGG	0.481006	1.3:-60940679	MS.gene20912:CDS
CCTCAAACCACTTCGGATAC+CGG	0.486692	1.3:+60940478	None:intergenic
AATCCAGGTTGCCATGGGAT+TGG	0.488234	1.3:-60940596	MS.gene20912:CDS
CTCCTATCAACAATGGTTCA+AGG	0.489683	1.3:-60940785	MS.gene20912:CDS
CGGATACCGGATTAAATCTC+CGG	0.495277	1.3:+60940491	None:intergenic
CAGGTTGCCATGGGATTGGC+AGG	0.499106	1.3:-60940592	MS.gene20912:CDS
GGATTGGCAGGTAGACCAAC+TGG	0.504664	1.3:-60940580	MS.gene20912:CDS
CCGGAAAATCCAGGTTGCCA+TGG	0.518583	1.3:-60940602	MS.gene20912:CDS
CACATTGTATAATTCTTCCA+AGG	0.523416	1.3:+60940695	None:intergenic
AAACTATAACGTTCTTGATA+CGG	0.530071	1.3:+60940435	None:intergenic
CGGAAAATCCAGGTTGCCAT+GGG	0.538481	1.3:-60940601	MS.gene20912:CDS
TATGGAAGACCGGAAAATCC+AGG	0.546447	1.3:-60940611	MS.gene20912:CDS
AATGAAGATAGAGAAATCCT+TGG	0.554986	1.3:-60940712	MS.gene20912:CDS
AGATGGTCAGTATGGAAGAC+CGG	0.563715	1.3:-60940621	MS.gene20912:CDS
CTCAGCTTGATGTTTCAAGA+TGG	0.565626	1.3:-60940638	MS.gene20912:CDS
CCGGTATCCGAAGTGGTTTG+AGG	0.566818	1.3:-60940478	MS.gene20912:CDS
AATAATCATCACCATATGCT+CGG	0.568249	1.3:+60940412	None:intergenic
TCAAGTAACTCTTCTATGCG+AGG	0.580396	1.3:-60940750	MS.gene20912:CDS
CAAGTAACTCTTCTATGCGA+GGG	0.589742	1.3:-60940749	MS.gene20912:CDS
CTTCTTCTATGTACACCAGT+TGG	0.594279	1.3:+60940565	None:intergenic
ACCTATTCTCCTATCAACAA+TGG	0.597081	1.3:-60940792	MS.gene20912:CDS
CAACTGGTGTACATAGAAGA+AGG	0.600561	1.3:-60940564	MS.gene20912:CDS
AAGTGAGAGTTGTTCCTGAA+GGG	0.600643	1.3:-60940539	MS.gene20912:CDS
GAAGTGAGAGTTGTTCCTGA+AGG	0.600726	1.3:-60940540	MS.gene20912:CDS
TGGTCTACCTGCCAATCCCA+TGG	0.603266	1.3:+60940585	None:intergenic
GGAAGAATTATACAATGTGA+AGG	0.609686	1.3:-60940691	MS.gene20912:CDS
ATTTAATCCGGTATCCGAAG+TGG	0.624736	1.3:-60940485	MS.gene20912:CDS
AGATCAGCCTCAAACCACTT+CGG	0.631094	1.3:+60940471	None:intergenic
GTTGATAGGAGAATAGGTGA+AGG	0.653020	1.3:+60940797	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACTATAACGTTCTTGATA+CGG	+	chr1.3:60940797-60940816	None:intergenic	25.0%
	AATAATCATCACCATATGCT+CGG	+	chr1.3:60940820-60940839	None:intergenic	30.0%
	AATGAAGATAGAGAAATCCT+TGG	-	chr1.3:60940517-60940536	MS.gene20912:CDS	30.0%
	CACATTGTATAATTCTTCCA+AGG	+	chr1.3:60940537-60940556	None:intergenic	30.0%
	GGAAGAATTATACAATGTGA+AGG	-	chr1.3:60940538-60940557	MS.gene20912:CDS	30.0%
	ACCTATTCTCCTATCAACAA+TGG	-	chr1.3:60940437-60940456	MS.gene20912:CDS	35.0%
	ATGTTTCAAGATGGTCAGTA+TGG	-	chr1.3:60940600-60940619	MS.gene20912:CDS	35.0%
	CAATGTGAAGGTAGAAAGAA+AGG	-	chr1.3:60940550-60940569	MS.gene20912:CDS	35.0%
!	ACCATTGTTGATAGGAGAAT+AGG	+	chr1.3:60940441-60940460	None:intergenic	35.0%
	AAGTGAGAGTTGTTCCTGAA+GGG	-	chr1.3:60940690-60940709	MS.gene20912:CDS	40.0%
	ATTTAATCCGGTATCCGAAG+TGG	-	chr1.3:60940744-60940763	MS.gene20912:CDS	40.0%
	CAACTGGTGTACATAGAAGA+AGG	-	chr1.3:60940665-60940684	MS.gene20912:CDS	40.0%
	CAAGTAACTCTTCTATGCGA+GGG	-	chr1.3:60940480-60940499	MS.gene20912:CDS	40.0%
	CGTTATAGTTTCCGAGCATA+TGG	-	chr1.3:60940806-60940825	MS.gene20912:CDS	40.0%
	CTCAGCTTGATGTTTCAAGA+TGG	-	chr1.3:60940591-60940610	MS.gene20912:CDS	40.0%
	CTCCTATCAACAATGGTTCA+AGG	-	chr1.3:60940444-60940463	MS.gene20912:CDS	40.0%
	CTCCTTGAACCATTGTTGAT+AGG	+	chr1.3:60940449-60940468	None:intergenic	40.0%
	CTTCTTCTATGTACACCAGT+TGG	+	chr1.3:60940667-60940686	None:intergenic	40.0%
	TCAAGTAACTCTTCTATGCG+AGG	-	chr1.3:60940479-60940498	MS.gene20912:CDS	40.0%
	TTGTTGCCGGAGATTTAATC+CGG	-	chr1.3:60940732-60940751	MS.gene20912:CDS	40.0%
!	GTTGATAGGAGAATAGGTGA+AGG	+	chr1.3:60940435-60940454	None:intergenic	40.0%
!!	CGTTTCATGCAATTTGTTGC+CGG	-	chr1.3:60940719-60940738	MS.gene20912:CDS	40.0%
!!!	TCAAGCTGAGCTAGTTTTTC+AGG	+	chr1.3:60940581-60940600	None:intergenic	40.0%
	AGATCAGCCTCAAACCACTT+CGG	+	chr1.3:60940761-60940780	None:intergenic	45.0%
	AGATGGTCAGTATGGAAGAC+CGG	-	chr1.3:60940608-60940627	MS.gene20912:CDS	45.0%
	CGGATACCGGATTAAATCTC+CGG	+	chr1.3:60940741-60940760	None:intergenic	45.0%
	GAAGTGAGAGTTGTTCCTGA+AGG	-	chr1.3:60940689-60940708	MS.gene20912:CDS	45.0%
	TATGGAAGACCGGAAAATCC+AGG	-	chr1.3:60940618-60940637	MS.gene20912:CDS	45.0%
!	AGTGGTTTGAGGCTGATCTT+TGG	-	chr1.3:60940762-60940781	MS.gene20912:CDS	45.0%
!	GAGGCTGATCTTTGGTTCAA+TGG	-	chr1.3:60940770-60940789	MS.gene20912:CDS	45.0%
	AATCCAGGTTGCCATGGGAT+TGG	-	chr1.3:60940633-60940652	MS.gene20912:CDS	50.0%
	ATGAAACGCTTGCTCCCTTC+AGG	+	chr1.3:60940707-60940726	None:intergenic	50.0%
	CCTCAAACCACTTCGGATAC+CGG	+	chr1.3:60940754-60940773	None:intergenic	50.0%
	CGGAAAATCCAGGTTGCCAT+GGG	-	chr1.3:60940628-60940647	MS.gene20912:CDS	50.0%
!	CCATGGCAACCTGGATTTTC+CGG	+	chr1.3:60940630-60940649	None:intergenic	50.0%
	CCGGAAAATCCAGGTTGCCA+TGG	-	chr1.3:60940627-60940646	MS.gene20912:CDS	55.0%
	CCGGTATCCGAAGTGGTTTG+AGG	-	chr1.3:60940751-60940770	MS.gene20912:CDS	55.0%
	TGGTCTACCTGCCAATCCCA+TGG	+	chr1.3:60940647-60940666	None:intergenic	55.0%
!	GGATTGGCAGGTAGACCAAC+TGG	-	chr1.3:60940649-60940668	MS.gene20912:CDS	55.0%
	CTGCCAATCCCATGGCAACC+TGG	+	chr1.3:60940639-60940658	None:intergenic	60.0%
!	CAGGTTGCCATGGGATTGGC+AGG	-	chr1.3:60940637-60940656	MS.gene20912:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	60940410	60940841	60940410	ID=MS.gene20912
chr1.3	mRNA	60940410	60940841	60940410	ID=MS.gene20912.t1;Parent=MS.gene20912
chr1.3	exon	60940410	60940841	60940410	ID=MS.gene20912.t1.exon1;Parent=MS.gene20912.t1
chr1.3	CDS	60940410	60940841	60940410	ID=cds.MS.gene20912.t1;Parent=MS.gene20912.t1

Gene Sequence

>MS.gene20912

ATGGCAAGCTTGTTGACATCAACCTTCACCTATTCTCCTATCAACAATGGTTCAAGGAGTTTGAGAAAATCAAGTAACTCTTCTATGCGAGGGTGTTGCGTAAAAGCAATGAAGATAGAGAAATCCTTGGAAGAATTATACAATGTGAAGGTAGAAAGAAAGGTGTCACCTGAAAAACTAGCTCAGCTTGATGTTTCAAGATGGTCAGTATGGAAGACCGGAAAATCCAGGTTGCCATGGGATTGGCAGGTAGACCAACTGGTGTACATAGAAGAAGGTGAAGTGAGAGTTGTTCCTGAAGGGAGCAAGCGTTTCATGCAATTTGTTGCCGGAGATTTAATCCGGTATCCGAAGTGGTTTGAGGCTGATCTTTGGTTCAATGGTCCGTATCAAGAACGTTATAGTTTCCGAGCATATGGTGATGATTATTGA

Protein sequence

>MS.gene20912.t1

MASLLTSTFTYSPINNGSRSLRKSSNSSMRGCCVKAMKIEKSLEELYNVKVERKVSPEKLAQLDVSRWSVWKTGKSRLPWDWQVDQLVYIEEGEVRVVPEGSKRFMQFVAGDLIRYPKWFEADLWFNGPYQERYSFRAYGDDY