AlfalfaGEDB | MS.gene07368

M. sativa cultivar XinJiangDaYe / MS.gene07368

KEGG: -
GO: -
PFAM: PF05899.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07368.t1	XP_003591303.1	99.3	143	1	0	1	143	1	143	6.90E-76	293.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07368.t1	G7IBQ3	99.3	143	1	0	1	143	1	143	5.0e-76	293.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene07368	0.849289	3.56E-60	-1.69E-46
MS.gene049378	MS.gene07368	0.800672	1.29E-48	-1.69E-46
MS.gene049758	MS.gene07368	0.829103	6.21E-55	-1.69E-46
MS.gene04987	MS.gene07368	0.824581	7.47E-54	-1.69E-46
MS.gene04988	MS.gene07368	0.803084	4.13E-49	-1.69E-46
MS.gene049915	MS.gene07368	0.80317	3.96E-49	-1.69E-46
MS.gene050226	MS.gene07368	0.822451	2.35E-53	-1.69E-46
MS.gene050227	MS.gene07368	0.838083	3.55E-57	-1.69E-46
MS.gene050228	MS.gene07368	0.829621	4.65E-55	-1.69E-46
MS.gene050277	MS.gene07368	0.837122	6.26E-57	-1.69E-46
MS.gene050303	MS.gene07368	0.874843	4.92E-68	-1.69E-46
MS.gene050330	MS.gene07368	-0.853119	2.95E-61	-1.69E-46
MS.gene050438	MS.gene07368	0.805718	1.17E-49	-1.69E-46
MS.gene050558	MS.gene07368	0.802947	4.40E-49	-1.69E-46
MS.gene050623	MS.gene07368	-0.856818	2.49E-62	-1.69E-46
MS.gene050642	MS.gene07368	0.86031	2.26E-63	-1.69E-46
MS.gene050719	MS.gene07368	0.802266	6.09E-49	-1.69E-46
MS.gene050721	MS.gene07368	0.805934	1.05E-49	-1.69E-46
MS.gene051257	MS.gene07368	0.826363	2.83E-54	-1.69E-46
MS.gene051349	MS.gene07368	0.810038	1.41E-50	-1.69E-46
MS.gene051633	MS.gene07368	0.83118	1.93E-55	-1.69E-46
MS.gene051662	MS.gene07368	0.829485	5.01E-55	-1.69E-46
MS.gene051663	MS.gene07368	0.808739	2.67E-50	-1.69E-46
MS.gene051983	MS.gene07368	0.84657	2.00E-59	-1.69E-46
MS.gene052048	MS.gene07368	-0.822629	2.14E-53	-1.69E-46
MS.gene052176	MS.gene07368	0.859442	4.12E-63	-1.69E-46
MS.gene052256	MS.gene07368	0.802922	4.46E-49	-1.69E-46
MS.gene052286	MS.gene07368	0.824388	8.29E-54	-1.69E-46
MS.gene052302	MS.gene07368	0.862422	5.12E-64	-1.69E-46
MS.gene052309	MS.gene07368	-0.884829	1.37E-71	-1.69E-46
MS.gene052358	MS.gene07368	-0.817335	3.48E-52	-1.69E-46
MS.gene052376	MS.gene07368	0.868993	4.32E-66	-1.69E-46
MS.gene052600	MS.gene07368	0.870275	1.65E-66	-1.69E-46
MS.gene052856	MS.gene07368	0.835837	1.33E-56	-1.69E-46
MS.gene052858	MS.gene07368	0.815834	7.55E-52	-1.69E-46
MS.gene052860	MS.gene07368	0.81291	3.34E-51	-1.69E-46
MS.gene053216	MS.gene07368	0.800574	1.35E-48	-1.69E-46
MS.gene053836	MS.gene07368	0.836362	9.79E-57	-1.69E-46
MS.gene054930	MS.gene07368	0.811915	5.51E-51	-1.69E-46
MS.gene054942	MS.gene07368	0.85507	8.07E-62	-1.69E-46
MS.gene054983	MS.gene07368	0.803051	4.19E-49	-1.69E-46
MS.gene055441	MS.gene07368	0.812557	3.99E-51	-1.69E-46
MS.gene055808	MS.gene07368	0.804444	2.15E-49	-1.69E-46
MS.gene056069	MS.gene07368	-0.801527	8.63E-49	-1.69E-46
MS.gene056091	MS.gene07368	0.803815	2.91E-49	-1.69E-46
MS.gene056631	MS.gene07368	0.839471	1.55E-57	-1.69E-46
MS.gene056979	MS.gene07368	0.835993	1.21E-56	-1.69E-46
MS.gene057060	MS.gene07368	0.815331	9.76E-52	-1.69E-46
MS.gene057079	MS.gene07368	0.844358	7.94E-59	-1.69E-46
MS.gene057121	MS.gene07368	0.815077	1.11E-51	-1.69E-46
MS.gene057140	MS.gene07368	0.818617	1.79E-52	-1.69E-46
MS.gene057260	MS.gene07368	0.822926	1.82E-53	-1.69E-46
MS.gene057555	MS.gene07368	0.832293	1.03E-55	-1.69E-46
MS.gene05795	MS.gene07368	0.815017	1.15E-51	-1.69E-46
MS.gene057967	MS.gene07368	0.817351	3.45E-52	-1.69E-46
MS.gene058182	MS.gene07368	0.8327	8.14E-56	-1.69E-46
MS.gene058201	MS.gene07368	0.817668	2.93E-52	-1.69E-46
MS.gene058238	MS.gene07368	0.846662	1.89E-59	-1.69E-46
MS.gene058399	MS.gene07368	0.81278	3.57E-51	-1.69E-46
MS.gene058906	MS.gene07368	0.816822	4.54E-52	-1.69E-46
MS.gene059249	MS.gene07368	0.801806	7.57E-49	-1.69E-46
MS.gene05954	MS.gene07368	0.824936	6.16E-54	-1.69E-46
MS.gene059569	MS.gene07368	0.822432	2.38E-53	-1.69E-46
MS.gene059899	MS.gene07368	0.810782	9.71E-51	-1.69E-46
MS.gene06001	MS.gene07368	0.831978	1.23E-55	-1.69E-46
MS.gene060165	MS.gene07368	0.818657	1.75E-52	-1.69E-46
MS.gene060297	MS.gene07368	0.861476	9.99E-64	-1.69E-46
MS.gene060476	MS.gene07368	0.818781	1.64E-52	-1.69E-46
MS.gene060572	MS.gene07368	0.805589	1.24E-49	-1.69E-46
MS.gene060586	MS.gene07368	0.833849	4.21E-56	-1.69E-46
MS.gene060619	MS.gene07368	0.827396	1.60E-54	-1.69E-46
MS.gene061040	MS.gene07368	-0.818827	1.60E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene07368	MS.gene20912	PPI
MS.gene21134	MS.gene07368	PPI
MS.gene07368	MS.gene005422	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07368.t1	MTR_1g086000	99.301	143	1	1	143	1	143	2.68e-104	294
MS.gene07368.t1	MTR_6g060170	40.260	77	46	42	118	3	79	2.42e-16	69.7
MS.gene07368.t1	MTR_1g076840	32.500	80	54	39	118	34	113	8.78e-13	61.6
MS.gene07368.t1	MTR_6g059920	32.258	93	63	45	137	36	128	2.24e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07368.t1	AT2G32650	67.133	143	42	2	1	142	1	139	4.04e-65	195
MS.gene07368.t1	AT2G32650	67.133	143	42	2	1	142	1	139	4.04e-65	195
MS.gene07368.t1	AT2G32180	67.133	143	42	2	1	142	1	139	5.03e-65	194
MS.gene07368.t1	AT2G32180	67.133	143	42	2	1	142	1	139	5.03e-65	194
MS.gene07368.t1	AT4G10290	35.065	77	47	1	45	118	16	92	2.83e-11	57.4
MS.gene07368.t1	AT4G10300	29.703	101	69	1	18	118	17	115	3.95e-11	57.4

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTTCATGCAATTTGTTGC+CGG	0.346254	1.2:-64687780	MS.gene07368:CDS
GAGGCTGATCTTTGGTTCAA+TGG	0.368609	1.2:-64687729	MS.gene07368:CDS
ATGTTTCAAGATGGTCAGTA+TGG	0.381328	1.2:-64687899	MS.gene07368:CDS
AGTGGTTTGAGGCTGATCTT+TGG	0.382114	1.2:-64687737	MS.gene07368:CDS
CGTTATAGTTTCCGAGCATA+TGG	0.389309	1.2:-64687693	MS.gene07368:CDS
ATGAAACGCTTGCTCCCTTC+AGG	0.397604	1.2:+64687795	None:intergenic
CTCCTTGAACCATTGTTGAT+AGG	0.416620	1.2:+64688053	None:intergenic
CTGCCAATCCCATGGCAACC+TGG	0.474702	1.2:+64687863	None:intergenic
ACCATTGTTGATAGGAGAAT+AGG	0.477458	1.2:+64688061	None:intergenic
TTGTTGCCGGAGATTTAATC+CGG	0.479684	1.2:-64687767	MS.gene07368:CDS
CAATGTGAAGGTAGAAAGAA+AGG	0.481006	1.2:-64687949	MS.gene07368:CDS
CCTCAAACCACTTCGGATAC+CGG	0.486692	1.2:+64687748	None:intergenic
AATCCAGGTTGCCATGGGAT+TGG	0.488234	1.2:-64687866	MS.gene07368:CDS
CTCCTATCAACAATGGTTCA+AGG	0.489683	1.2:-64688055	MS.gene07368:CDS
CGGATACCGGATTAAATCTC+CGG	0.495277	1.2:+64687761	None:intergenic
CAGGTTGCCATGGGATTGGC+AGG	0.499106	1.2:-64687862	MS.gene07368:CDS
GGATTGGCAGGTAGACCAAC+TGG	0.504664	1.2:-64687850	MS.gene07368:CDS
CCGGAAAATCCAGGTTGCCA+TGG	0.518583	1.2:-64687872	MS.gene07368:CDS
CACATTGTATAATTCTTCCA+AGG	0.523416	1.2:+64687965	None:intergenic
AAACTATAACGTTCTTGATA+CGG	0.530071	1.2:+64687705	None:intergenic
CGGAAAATCCAGGTTGCCAT+GGG	0.538481	1.2:-64687871	MS.gene07368:CDS
TATGGAAGACCGGAAAATCC+AGG	0.546447	1.2:-64687881	MS.gene07368:CDS
AATGAAGATAGAGAAATCCT+TGG	0.554986	1.2:-64687982	MS.gene07368:CDS
AGATGGTCAGTATGGAAGAC+CGG	0.563715	1.2:-64687891	MS.gene07368:CDS
CTCAGCTTGATGTTTCAAGA+TGG	0.565626	1.2:-64687908	MS.gene07368:CDS
CCGGTATCCGAAGTGGTTTG+AGG	0.566818	1.2:-64687748	MS.gene07368:CDS
AATAATCATCACCATATGCT+CGG	0.568249	1.2:+64687682	None:intergenic
TCAAGTAACTCTTCTATGCG+AGG	0.580396	1.2:-64688020	MS.gene07368:CDS
CAAGTAACTCTTCTATGCGA+GGG	0.589742	1.2:-64688019	MS.gene07368:CDS
CTTCTTCTATGTACACCAGT+TGG	0.594279	1.2:+64687835	None:intergenic
ACCTATTCTCCTATCAACAA+TGG	0.597081	1.2:-64688062	MS.gene07368:CDS
CAACTGGTGTACATAGAAGA+AGG	0.600561	1.2:-64687834	MS.gene07368:CDS
AAGTGAGAGTTGTTCCTGAA+GGG	0.600643	1.2:-64687809	MS.gene07368:CDS
GAAGTGAGAGTTGTTCCTGA+AGG	0.600726	1.2:-64687810	MS.gene07368:CDS
TGGTCTACCTGCCAATCCCA+TGG	0.603266	1.2:+64687855	None:intergenic
GGAAGAATTATACAATGTGA+AGG	0.609686	1.2:-64687961	MS.gene07368:CDS
ATTTAATCCGGTATCCGAAG+TGG	0.624736	1.2:-64687755	MS.gene07368:CDS
AGATCAGCCTCAAACCACTT+CGG	0.631094	1.2:+64687741	None:intergenic
GTTGATAGGAGAATAGGTGA+AGG	0.653020	1.2:+64688067	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACTATAACGTTCTTGATA+CGG	+	chr1.2:64688067-64688086	None:intergenic	25.0%
	AATAATCATCACCATATGCT+CGG	+	chr1.2:64688090-64688109	None:intergenic	30.0%
	AATGAAGATAGAGAAATCCT+TGG	-	chr1.2:64687787-64687806	MS.gene07368:CDS	30.0%
	CACATTGTATAATTCTTCCA+AGG	+	chr1.2:64687807-64687826	None:intergenic	30.0%
	GGAAGAATTATACAATGTGA+AGG	-	chr1.2:64687808-64687827	MS.gene07368:CDS	30.0%
	ACCTATTCTCCTATCAACAA+TGG	-	chr1.2:64687707-64687726	MS.gene07368:CDS	35.0%
	ATGTTTCAAGATGGTCAGTA+TGG	-	chr1.2:64687870-64687889	MS.gene07368:CDS	35.0%
	CAATGTGAAGGTAGAAAGAA+AGG	-	chr1.2:64687820-64687839	MS.gene07368:CDS	35.0%
!	ACCATTGTTGATAGGAGAAT+AGG	+	chr1.2:64687711-64687730	None:intergenic	35.0%
	AAGTGAGAGTTGTTCCTGAA+GGG	-	chr1.2:64687960-64687979	MS.gene07368:CDS	40.0%
	ATTTAATCCGGTATCCGAAG+TGG	-	chr1.2:64688014-64688033	MS.gene07368:CDS	40.0%
	CAACTGGTGTACATAGAAGA+AGG	-	chr1.2:64687935-64687954	MS.gene07368:CDS	40.0%
	CAAGTAACTCTTCTATGCGA+GGG	-	chr1.2:64687750-64687769	MS.gene07368:CDS	40.0%
	CGTTATAGTTTCCGAGCATA+TGG	-	chr1.2:64688076-64688095	MS.gene07368:CDS	40.0%
	CTCAGCTTGATGTTTCAAGA+TGG	-	chr1.2:64687861-64687880	MS.gene07368:CDS	40.0%
	CTCCTATCAACAATGGTTCA+AGG	-	chr1.2:64687714-64687733	MS.gene07368:CDS	40.0%
	CTCCTTGAACCATTGTTGAT+AGG	+	chr1.2:64687719-64687738	None:intergenic	40.0%
	CTTCTTCTATGTACACCAGT+TGG	+	chr1.2:64687937-64687956	None:intergenic	40.0%
	TCAAGTAACTCTTCTATGCG+AGG	-	chr1.2:64687749-64687768	MS.gene07368:CDS	40.0%
	TTGTTGCCGGAGATTTAATC+CGG	-	chr1.2:64688002-64688021	MS.gene07368:CDS	40.0%
!	GTTGATAGGAGAATAGGTGA+AGG	+	chr1.2:64687705-64687724	None:intergenic	40.0%
!!	CGTTTCATGCAATTTGTTGC+CGG	-	chr1.2:64687989-64688008	MS.gene07368:CDS	40.0%
!!!	TCAAGCTGAGCTAGTTTTTC+AGG	+	chr1.2:64687851-64687870	None:intergenic	40.0%
	AGATCAGCCTCAAACCACTT+CGG	+	chr1.2:64688031-64688050	None:intergenic	45.0%
	AGATGGTCAGTATGGAAGAC+CGG	-	chr1.2:64687878-64687897	MS.gene07368:CDS	45.0%
	CGGATACCGGATTAAATCTC+CGG	+	chr1.2:64688011-64688030	None:intergenic	45.0%
	GAAGTGAGAGTTGTTCCTGA+AGG	-	chr1.2:64687959-64687978	MS.gene07368:CDS	45.0%
	TATGGAAGACCGGAAAATCC+AGG	-	chr1.2:64687888-64687907	MS.gene07368:CDS	45.0%
!	AGTGGTTTGAGGCTGATCTT+TGG	-	chr1.2:64688032-64688051	MS.gene07368:CDS	45.0%
!	GAGGCTGATCTTTGGTTCAA+TGG	-	chr1.2:64688040-64688059	MS.gene07368:CDS	45.0%
	AATCCAGGTTGCCATGGGAT+TGG	-	chr1.2:64687903-64687922	MS.gene07368:CDS	50.0%
	ATGAAACGCTTGCTCCCTTC+AGG	+	chr1.2:64687977-64687996	None:intergenic	50.0%
	CCTCAAACCACTTCGGATAC+CGG	+	chr1.2:64688024-64688043	None:intergenic	50.0%
	CGGAAAATCCAGGTTGCCAT+GGG	-	chr1.2:64687898-64687917	MS.gene07368:CDS	50.0%
!	CCATGGCAACCTGGATTTTC+CGG	+	chr1.2:64687900-64687919	None:intergenic	50.0%
	CCGGAAAATCCAGGTTGCCA+TGG	-	chr1.2:64687897-64687916	MS.gene07368:CDS	55.0%
	CCGGTATCCGAAGTGGTTTG+AGG	-	chr1.2:64688021-64688040	MS.gene07368:CDS	55.0%
	TGGTCTACCTGCCAATCCCA+TGG	+	chr1.2:64687917-64687936	None:intergenic	55.0%
!	GGATTGGCAGGTAGACCAAC+TGG	-	chr1.2:64687919-64687938	MS.gene07368:CDS	55.0%
	CTGCCAATCCCATGGCAACC+TGG	+	chr1.2:64687909-64687928	None:intergenic	60.0%
!	CAGGTTGCCATGGGATTGGC+AGG	-	chr1.2:64687907-64687926	MS.gene07368:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	64687680	64688111	64687680	ID=MS.gene07368
chr1.2	mRNA	64687680	64688111	64687680	ID=MS.gene07368.t1;Parent=MS.gene07368
chr1.2	exon	64687680	64688111	64687680	ID=MS.gene07368.t1.exon1;Parent=MS.gene07368.t1
chr1.2	CDS	64687680	64688111	64687680	ID=cds.MS.gene07368.t1;Parent=MS.gene07368.t1

Gene Sequence

>MS.gene07368

ATGGCAAGCTTGTTGACATCAACCTTCACCTATTCTCCTATCAACAATGGTTCAAGGAGTTTGAGAAAATCAAGTAACTCTTCTATGCGAGGGTGTTGCGTAAAAGCAATGAAGATAGAGAAATCCTTGGAAGAATTATACAATGTGAAGGTAGAAAGAAAGGTGTCACCTGAAAAACTAGCTCAGCTTGATGTTTCAAGATGGTCAGTATGGAAGACCGGAAAATCCAGGTTGCCATGGGATTGGCAGGTAGACCAACTGGTGTACATAGAAGAAGGTGAAGTGAGAGTTGTTCCTGAAGGGAGCAAGCGTTTCATGCAATTTGTTGCCGGAGATTTAATCCGGTATCCGAAGTGGTTTGAGGCTGATCTTTGGTTCAATGGTCCGTATCAAGAACGTTATAGTTTCCGAGCATATGGTGATGATTATTGA

Protein sequence

>MS.gene07368.t1

MASLLTSTFTYSPINNGSRSLRKSSNSSMRGCCVKAMKIEKSLEELYNVKVERKVSPEKLAQLDVSRWSVWKTGKSRLPWDWQVDQLVYIEEGEVRVVPEGSKRFMQFVAGDLIRYPKWFEADLWFNGPYQERYSFRAYGDDY