AlfalfaGEDB | MS.gene21134

M. sativa cultivar XinJiangDaYe / MS.gene21134

KEGG: -
GO: -
PFAM: PF05899.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21134.t1	XP_003591303.1	98.6	143	2	0	1	143	1	143	3.40E-75	290.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21134.t1	G7IBQ3	98.6	143	2	0	1	143	1	143	2.5e-75	290.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene21134	0.847655	1.01E-59	-1.69E-46
MS.gene049758	MS.gene21134	0.824374	8.35E-54	-1.69E-46
MS.gene050226	MS.gene21134	0.800127	1.67E-48	-1.69E-46
MS.gene050227	MS.gene21134	0.817798	2.74E-52	-1.69E-46
MS.gene050228	MS.gene21134	0.808592	2.87E-50	-1.69E-46
MS.gene050277	MS.gene21134	0.804455	2.14E-49	-1.69E-46
MS.gene050303	MS.gene21134	0.857097	2.06E-62	-1.69E-46
MS.gene050330	MS.gene21134	-0.819017	1.45E-52	-1.69E-46
MS.gene050558	MS.gene21134	0.811935	5.46E-51	-1.69E-46
MS.gene050623	MS.gene21134	-0.834997	2.17E-56	-1.69E-46
MS.gene050642	MS.gene21134	0.86003	2.74E-63	-1.69E-46
MS.gene050820	MS.gene21134	0.801397	9.18E-49	-1.69E-46
MS.gene051240	MS.gene21134	0.816505	5.34E-52	-1.69E-46
MS.gene051257	MS.gene21134	0.824245	8.96E-54	-1.69E-46
MS.gene051662	MS.gene21134	0.834857	2.35E-56	-1.69E-46
MS.gene051663	MS.gene21134	0.816529	5.28E-52	-1.69E-46
MS.gene051983	MS.gene21134	0.847526	1.09E-59	-1.69E-46
MS.gene052176	MS.gene21134	0.831329	1.78E-55	-1.69E-46
MS.gene052302	MS.gene21134	0.822201	2.69E-53	-1.69E-46
MS.gene052309	MS.gene21134	-0.854408	1.26E-61	-1.69E-46
MS.gene052376	MS.gene21134	0.831169	1.94E-55	-1.69E-46
MS.gene052600	MS.gene21134	0.87567	2.57E-68	-1.69E-46
MS.gene053836	MS.gene21134	0.853534	2.24E-61	-1.69E-46
MS.gene053971	MS.gene21134	0.803058	4.18E-49	-1.69E-46
MS.gene054185	MS.gene21134	0.806353	8.57E-50	-1.69E-46
MS.gene054942	MS.gene21134	0.849098	4.02E-60	-1.69E-46
MS.gene054983	MS.gene21134	0.823364	1.44E-53	-1.69E-46
MS.gene055422	MS.gene21134	0.801142	1.04E-48	-1.69E-46
MS.gene056612	MS.gene21134	0.809698	1.66E-50	-1.69E-46
MS.gene056979	MS.gene21134	0.834412	3.04E-56	-1.69E-46
MS.gene057121	MS.gene21134	0.843532	1.32E-58	-1.69E-46
MS.gene057260	MS.gene21134	0.818636	1.77E-52	-1.69E-46
MS.gene057439	MS.gene21134	0.828591	8.26E-55	-1.69E-46
MS.gene058002	MS.gene21134	0.807875	4.08E-50	-1.69E-46
MS.gene05811	MS.gene21134	0.822107	2.83E-53	-1.69E-46
MS.gene058201	MS.gene21134	0.813235	2.84E-51	-1.69E-46
MS.gene058749	MS.gene21134	0.808471	3.05E-50	-1.69E-46
MS.gene058833	MS.gene21134	0.802467	5.53E-49	-1.69E-46
MS.gene058845	MS.gene21134	0.811258	7.66E-51	-1.69E-46
MS.gene059569	MS.gene21134	0.800655	1.30E-48	-1.69E-46
MS.gene05984	MS.gene21134	0.827323	1.67E-54	-1.69E-46
MS.gene060165	MS.gene21134	0.802523	5.39E-49	-1.69E-46
MS.gene060297	MS.gene21134	0.800733	1.25E-48	-1.69E-46
MS.gene060476	MS.gene21134	0.813956	1.97E-51	-1.69E-46
MS.gene060619	MS.gene21134	0.806377	8.47E-50	-1.69E-46
MS.gene060994	MS.gene21134	0.802084	6.63E-49	-1.69E-46
MS.gene061040	MS.gene21134	-0.801951	7.06E-49	-1.69E-46
MS.gene061049	MS.gene21134	0.805788	1.13E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21134	MS.gene20912	PPI
MS.gene21134	MS.gene005422	PPI
MS.gene21134	MS.gene07368	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21134.t1	MTR_1g086000	98.601	143	2	1	143	1	143	1.71e-103	292
MS.gene21134.t1	MTR_6g060170	38.961	77	47	42	118	3	79	1.43e-15	67.8
MS.gene21134.t1	MTR_1g076840	31.250	80	55	39	118	34	113	7.73e-12	59.3
MS.gene21134.t1	MTR_6g059920	31.183	93	64	45	137	36	128	1.22e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21134.t1	AT2G32650	66.434	143	43	2	1	142	1	139	2.93e-64	192
MS.gene21134.t1	AT2G32650	66.434	143	43	2	1	142	1	139	2.93e-64	192
MS.gene21134.t1	AT2G32180	66.434	143	43	2	1	142	1	139	3.13e-64	192
MS.gene21134.t1	AT2G32180	66.434	143	43	2	1	142	1	139	3.13e-64	192
MS.gene21134.t1	AT4G10290	35.065	77	47	1	45	118	16	92	1.30e-11	58.2

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTTTCATGCAATTTGTTGC+CGG	0.346254	1.4:-67940479	MS.gene21134:CDS
GAGGCTGATCTTTGGTTCAA+TGG	0.368609	1.4:-67940428	MS.gene21134:CDS
ATGTTTCAAGATGGTCAGTA+TGG	0.381328	1.4:-67940598	MS.gene21134:CDS
AGTGGTTTGAGGCTGATCTT+TGG	0.382114	1.4:-67940436	MS.gene21134:CDS
CGTTATAGTTTCCGAGCATA+TGG	0.389309	1.4:-67940392	MS.gene21134:CDS
ATGAAACGCTTGCTCCCTTC+AGG	0.397604	1.4:+67940494	None:intergenic
CTCCTTGAACCATTGTTGAT+AGG	0.416620	1.4:+67940752	None:intergenic
CAATGTGAAGGTAGCAAGAA+AGG	0.452172	1.4:-67940648	MS.gene21134:CDS
CTGCCAATCCCATGGCAACC+TGG	0.474702	1.4:+67940562	None:intergenic
ACCATTGTTGATAGGAGAAT+AGG	0.477458	1.4:+67940760	None:intergenic
TTGTTGCCGGAGATTTAATC+CGG	0.479684	1.4:-67940466	MS.gene21134:CDS
CCTCAAACCACTTCGGATAC+CGG	0.486692	1.4:+67940447	None:intergenic
AATCCAGGTTGCCATGGGAT+TGG	0.488234	1.4:-67940565	MS.gene21134:CDS
CTCCTATCAACAATGGTTCA+AGG	0.489683	1.4:-67940754	MS.gene21134:CDS
CGGATACCGGATTAAATCTC+CGG	0.495277	1.4:+67940460	None:intergenic
CAGGTTGCCATGGGATTGGC+AGG	0.499106	1.4:-67940561	MS.gene21134:CDS
GGATTGGCAGGTAGACCAAC+TGG	0.504664	1.4:-67940549	MS.gene21134:CDS
CCGGAAAATCCAGGTTGCCA+TGG	0.518583	1.4:-67940571	MS.gene21134:CDS
CACATTGTATAATTCTTCCA+AGG	0.523416	1.4:+67940664	None:intergenic
AAACTATAACGTTCTTGATA+CGG	0.530071	1.4:+67940404	None:intergenic
CGGAAAATCCAGGTTGCCAT+GGG	0.538481	1.4:-67940570	MS.gene21134:CDS
TATGGAAGACCGGAAAATCC+AGG	0.546447	1.4:-67940580	MS.gene21134:CDS
AATGAAGATAGAGAAATCCT+TGG	0.554986	1.4:-67940681	MS.gene21134:CDS
AGATGGTCAGTATGGAAGAC+CGG	0.563715	1.4:-67940590	MS.gene21134:CDS
CTCAGCTTGATGTTTCAAGA+TGG	0.565626	1.4:-67940607	MS.gene21134:CDS
CCGGTATCCGAAGTGGTTTG+AGG	0.566818	1.4:-67940447	MS.gene21134:CDS
AATAATCATCACCATATGCT+CGG	0.568249	1.4:+67940381	None:intergenic
TCAAGTAACTCTTCTATGCG+AGG	0.580396	1.4:-67940719	MS.gene21134:CDS
CAAGTAACTCTTCTATGCGA+GGG	0.589742	1.4:-67940718	MS.gene21134:CDS
CTTCTTCTATGTACACCAGT+TGG	0.594279	1.4:+67940534	None:intergenic
ACCTATTCTCCTATCAACAA+TGG	0.597081	1.4:-67940761	MS.gene21134:CDS
CAACTGGTGTACATAGAAGA+AGG	0.600561	1.4:-67940533	MS.gene21134:CDS
AAGTGAGAGTTGTTCCTGAA+GGG	0.600643	1.4:-67940508	MS.gene21134:CDS
GAAGTGAGAGTTGTTCCTGA+AGG	0.600726	1.4:-67940509	MS.gene21134:CDS
TGGTCTACCTGCCAATCCCA+TGG	0.603266	1.4:+67940554	None:intergenic
GGAAGAATTATACAATGTGA+AGG	0.609686	1.4:-67940660	MS.gene21134:CDS
ATTTAATCCGGTATCCGAAG+TGG	0.624736	1.4:-67940454	MS.gene21134:CDS
AGATCAGCCTCAAACCACTT+CGG	0.631094	1.4:+67940440	None:intergenic
GTTGATAGGAGAATAGGTGA+AGG	0.653020	1.4:+67940766	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACTATAACGTTCTTGATA+CGG	+	chr1.4:67940766-67940785	None:intergenic	25.0%
	AATAATCATCACCATATGCT+CGG	+	chr1.4:67940789-67940808	None:intergenic	30.0%
	AATGAAGATAGAGAAATCCT+TGG	-	chr1.4:67940486-67940505	MS.gene21134:CDS	30.0%
	CACATTGTATAATTCTTCCA+AGG	+	chr1.4:67940506-67940525	None:intergenic	30.0%
	GGAAGAATTATACAATGTGA+AGG	-	chr1.4:67940507-67940526	MS.gene21134:CDS	30.0%
	ACCTATTCTCCTATCAACAA+TGG	-	chr1.4:67940406-67940425	MS.gene21134:CDS	35.0%
	ATGTTTCAAGATGGTCAGTA+TGG	-	chr1.4:67940569-67940588	MS.gene21134:CDS	35.0%
!	ACCATTGTTGATAGGAGAAT+AGG	+	chr1.4:67940410-67940429	None:intergenic	35.0%
	AAGTGAGAGTTGTTCCTGAA+GGG	-	chr1.4:67940659-67940678	MS.gene21134:CDS	40.0%
	ATTTAATCCGGTATCCGAAG+TGG	-	chr1.4:67940713-67940732	MS.gene21134:CDS	40.0%
	CAACTGGTGTACATAGAAGA+AGG	-	chr1.4:67940634-67940653	MS.gene21134:CDS	40.0%
	CAAGTAACTCTTCTATGCGA+GGG	-	chr1.4:67940449-67940468	MS.gene21134:CDS	40.0%
	CAATGTGAAGGTAGCAAGAA+AGG	-	chr1.4:67940519-67940538	MS.gene21134:CDS	40.0%
	CGTTATAGTTTCCGAGCATA+TGG	-	chr1.4:67940775-67940794	MS.gene21134:CDS	40.0%
	CTCAGCTTGATGTTTCAAGA+TGG	-	chr1.4:67940560-67940579	MS.gene21134:CDS	40.0%
	CTCCTATCAACAATGGTTCA+AGG	-	chr1.4:67940413-67940432	MS.gene21134:CDS	40.0%
	CTCCTTGAACCATTGTTGAT+AGG	+	chr1.4:67940418-67940437	None:intergenic	40.0%
	CTTCTTCTATGTACACCAGT+TGG	+	chr1.4:67940636-67940655	None:intergenic	40.0%
	TCAAGTAACTCTTCTATGCG+AGG	-	chr1.4:67940448-67940467	MS.gene21134:CDS	40.0%
	TTGTTGCCGGAGATTTAATC+CGG	-	chr1.4:67940701-67940720	MS.gene21134:CDS	40.0%
!	GTTGATAGGAGAATAGGTGA+AGG	+	chr1.4:67940404-67940423	None:intergenic	40.0%
!!	CGTTTCATGCAATTTGTTGC+CGG	-	chr1.4:67940688-67940707	MS.gene21134:CDS	40.0%
!!!	TCAAGCTGAGCTAGTTTTTC+AGG	+	chr1.4:67940550-67940569	None:intergenic	40.0%
	AGATCAGCCTCAAACCACTT+CGG	+	chr1.4:67940730-67940749	None:intergenic	45.0%
	AGATGGTCAGTATGGAAGAC+CGG	-	chr1.4:67940577-67940596	MS.gene21134:CDS	45.0%
	CGGATACCGGATTAAATCTC+CGG	+	chr1.4:67940710-67940729	None:intergenic	45.0%
	GAAGTGAGAGTTGTTCCTGA+AGG	-	chr1.4:67940658-67940677	MS.gene21134:CDS	45.0%
	TATGGAAGACCGGAAAATCC+AGG	-	chr1.4:67940587-67940606	MS.gene21134:CDS	45.0%
!	AGTGGTTTGAGGCTGATCTT+TGG	-	chr1.4:67940731-67940750	MS.gene21134:CDS	45.0%
!	GAGGCTGATCTTTGGTTCAA+TGG	-	chr1.4:67940739-67940758	MS.gene21134:CDS	45.0%
	AATCCAGGTTGCCATGGGAT+TGG	-	chr1.4:67940602-67940621	MS.gene21134:CDS	50.0%
	ATGAAACGCTTGCTCCCTTC+AGG	+	chr1.4:67940676-67940695	None:intergenic	50.0%
	CCTCAAACCACTTCGGATAC+CGG	+	chr1.4:67940723-67940742	None:intergenic	50.0%
	CGGAAAATCCAGGTTGCCAT+GGG	-	chr1.4:67940597-67940616	MS.gene21134:CDS	50.0%
!	CCATGGCAACCTGGATTTTC+CGG	+	chr1.4:67940599-67940618	None:intergenic	50.0%
	CCGGAAAATCCAGGTTGCCA+TGG	-	chr1.4:67940596-67940615	MS.gene21134:CDS	55.0%
	CCGGTATCCGAAGTGGTTTG+AGG	-	chr1.4:67940720-67940739	MS.gene21134:CDS	55.0%
	TGGTCTACCTGCCAATCCCA+TGG	+	chr1.4:67940616-67940635	None:intergenic	55.0%
!	GGATTGGCAGGTAGACCAAC+TGG	-	chr1.4:67940618-67940637	MS.gene21134:CDS	55.0%
	CTGCCAATCCCATGGCAACC+TGG	+	chr1.4:67940608-67940627	None:intergenic	60.0%
!	CAGGTTGCCATGGGATTGGC+AGG	-	chr1.4:67940606-67940625	MS.gene21134:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	67940379	67940810	67940379	ID=MS.gene21134
chr1.4	mRNA	67940379	67940810	67940379	ID=MS.gene21134.t1;Parent=MS.gene21134
chr1.4	exon	67940379	67940810	67940379	ID=MS.gene21134.t1.exon1;Parent=MS.gene21134.t1
chr1.4	CDS	67940379	67940810	67940379	ID=cds.MS.gene21134.t1;Parent=MS.gene21134.t1

Gene Sequence

>MS.gene21134

ATGGCAAGCTTGTTGACATCAACCTTCACCTATTCTCCTATCAACAATGGTTCAAGGAGTTTGAGAAAATCAAGTAACTCTTCTATGCGAGGGTGTTGCGTAAAAGCAATGAAGATAGAGAAATCCTTGGAAGAATTATACAATGTGAAGGTAGCAAGAAAGGTGTCACCTGAAAAACTAGCTCAGCTTGATGTTTCAAGATGGTCAGTATGGAAGACCGGAAAATCCAGGTTGCCATGGGATTGGCAGGTAGACCAACTGGTGTACATAGAAGAAGGTGAAGTGAGAGTTGTTCCTGAAGGGAGCAAGCGTTTCATGCAATTTGTTGCCGGAGATTTAATCCGGTATCCGAAGTGGTTTGAGGCTGATCTTTGGTTCAATGGTCCGTATCAAGAACGTTATAGTTTCCGAGCATATGGTGATGATTATTGA

Protein sequence

>MS.gene21134.t1

MASLLTSTFTYSPINNGSRSLRKSSNSSMRGCCVKAMKIEKSLEELYNVKVARKVSPEKLAQLDVSRWSVWKTGKSRLPWDWQVDQLVYIEEGEVRVVPEGSKRFMQFVAGDLIRYPKWFEADLWFNGPYQERYSFRAYGDDY