AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005686.t1 MTR_1g089980 91.729 399 32 1 1 398 1 399 0.0 740
MS.gene005686.t1 MTR_7g094470 57.656 418 169 3 2 412 1 417 5.20e-166 472
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005686.t1 AT4G02920 38.061 423 240 9 5 412 3 418 1.09e-82 259
MS.gene005686.t1 AT4G02920 38.152 422 240 10 5 412 3 417 2.34e-82 259
MS.gene005686.t1 AT1G03340 35.000 360 199 8 56 412 58 385 9.30e-54 183

Find 78 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACACAGGGTTGGGTTT+TGG 0.134883 1.2:-67379551 MS.gene005686:CDS
TTGGGCTTATTTGGCTTTCT+TGG 0.139996 1.2:-67379532 MS.gene005686:CDS
GAGATGTAGAAATTCCTATT+TGG 0.209369 1.2:+67381399 None:intergenic
AAATGGTCAAGTCACGTTTA+TGG 0.221667 1.2:-67381316 MS.gene005686:CDS
CACTGACCGTTCCTTTAAAA+AGG 0.233508 1.2:-67380285 MS.gene005686:intron
TTGATGGGTTTGCAACTATC+AGG 0.248405 1.2:-67381462 MS.gene005686:CDS
TGGGCTTATTTGGCTTTCTT+GGG 0.252384 1.2:-67379531 MS.gene005686:CDS
GATATCAGAGTCCAAATCTT+AGG 0.281870 1.2:-67382064 MS.gene005686:CDS
AAAATTAATGTTCATCTAAA+TGG 0.282017 1.2:-67381333 MS.gene005686:CDS
GATGAATTTAACCTAAGATT+TGG 0.287708 1.2:+67382053 None:intergenic
TGAGAGCCTTCTGTTCTTGA+TGG 0.290630 1.2:-67379750 MS.gene005686:CDS
GATTCAGAAACTTACTTTGA+TGG 0.292699 1.2:-67382262 MS.gene005686:CDS
CAATTCTCTGCTGGCATTGC+TGG 0.299676 1.2:-67379650 MS.gene005686:CDS
ATTCTCTCATCTACCCAAAT+AGG 0.325349 1.2:-67381413 MS.gene005686:CDS
CTCTGATATCTAATAATATC+TGG 0.353756 1.2:+67382077 None:intergenic
GGATTGGCTTTGACTTTCTT+CGG 0.356219 1.2:+67379842 None:intergenic
TATCTAATAATATCTGGTTT+TGG 0.360696 1.2:+67382083 None:intergenic
AGCTTTGTTGTCTGACTTGA+TGG 0.364159 1.2:-67381478 MS.gene005686:CDS
AATGGTCAAGTCACGTTTAT+GGG 0.369340 1.2:-67381315 MS.gene005686:CDS
GTTCTTTCACTGAAGAAATC+AGG 0.382585 1.2:-67379698 MS.gene005686:CDS
AGATGTAGAAATTCCTATTT+GGG 0.383642 1.2:+67381400 None:intergenic
TCTGCTGGCATTGCTGGTAC+TGG 0.385638 1.2:-67379644 MS.gene005686:CDS
AAGGTCTTCAACACAGGGTT+GGG 0.391495 1.2:-67379557 MS.gene005686:CDS
ATGATAGGGTTGTCAACAAT+TGG 0.402315 1.2:-67382120 MS.gene005686:intron
TGTACATACCTAGTGAAGTA+TGG 0.405041 1.2:+67381198 None:intergenic
AAGCAAGAATGCTGGAAAGA+TGG 0.436409 1.2:-67379474 MS.gene005686:CDS
AGAGAATGCAATGATATTTG+AGG 0.446652 1.2:+67381429 None:intergenic
AGCAAGAATGCTGGAAAGAT+GGG 0.449740 1.2:-67379473 MS.gene005686:CDS
TGTTCTTGATGGCTGATAAG+AGG 0.453397 1.2:-67379739 MS.gene005686:CDS
GAGAATTGTGTAAGGAGCTC+GGG 0.454630 1.2:+67379665 None:intergenic
GAAGACCTTAGACGCACAAA+AGG 0.465886 1.2:+67379571 None:intergenic
ACTGCTGAAGCAGGTGTAGA+TGG 0.468818 1.2:-67381510 MS.gene005686:CDS
GCGTCTAAGGTCTTCAACAC+AGG 0.476430 1.2:-67379563 MS.gene005686:CDS
CTTACTTTGATGGCTTCTCA+TGG 0.481501 1.2:-67382252 MS.gene005686:CDS
GGCTGATAAGAGGCATCGTC+GGG 0.482476 1.2:-67379729 MS.gene005686:CDS
TTCACTGAAGAAATCAGGTC+CGG 0.487415 1.2:-67379693 MS.gene005686:CDS
TGCTAGTAGTGGCAGTGCTA+AGG 0.499557 1.2:-67379379 MS.gene005686:CDS
CTCCTTACACAATTCTCTGC+TGG 0.505017 1.2:-67379659 MS.gene005686:CDS
GGGCTTATTTGGCTTTCTTG+GGG 0.508276 1.2:-67379530 MS.gene005686:CDS
GTAAGGAGCTCGGGAAGCTC+CGG 0.511742 1.2:+67379674 None:intergenic
GTATCAAAACGCACACATAC+AGG 0.515015 1.2:-67381243 MS.gene005686:CDS
AAGACCTTAGACGCACAAAA+GGG 0.516732 1.2:+67379572 None:intergenic
TTCATGGCATATTCTTGAGA+TGG 0.519316 1.2:+67382225 None:intergenic
AAGCTGAAAAGTACAGTGCT+TGG 0.520108 1.2:+67381591 None:intergenic
TAAGGTCTTCAACACAGGGT+TGG 0.534621 1.2:-67379558 MS.gene005686:CDS
AGAGAATTGTGTAAGGAGCT+CGG 0.542826 1.2:+67379664 None:intergenic
GTAGAGTGAATATCCAATGC+GGG 0.543199 1.2:+67380307 None:intergenic
AAAAGAAAGTAGCCAGGGAG+AGG 0.544026 1.2:-67379805 MS.gene005686:CDS
ATCTCAAGAATATGCCATGA+AGG 0.545054 1.2:-67382223 MS.gene005686:intron
CTTGAACAGATCCCTCTCCC+TGG 0.545602 1.2:+67379793 None:intergenic
ACATCTGAGACTGCTGAAGC+AGG 0.548211 1.2:-67381519 MS.gene005686:CDS
AGCTGCAGCGTTGCTAGTAG+TGG 0.549465 1.2:-67379390 MS.gene005686:CDS
GTTAACATAAGCAAGAATGC+TGG 0.550226 1.2:-67379482 MS.gene005686:CDS
TGATGGGTTTGCAACTATCA+GGG 0.550930 1.2:-67381461 MS.gene005686:CDS
CCAACGGTTATTGATGTACA+AGG 0.561376 1.2:-67381954 MS.gene005686:intron
GCTTTGTTGTCTGACTTGAT+GGG 0.563096 1.2:-67381477 MS.gene005686:CDS
CCTTGTACATCAATAACCGT+TGG 0.573161 1.2:+67381954 None:intergenic
TGCCAGCAGAGAATTGTGTA+AGG 0.578242 1.2:+67379657 None:intergenic
GCTGAATATAAATGAAGTAG+AGG 0.580301 1.2:-67380344 MS.gene005686:CDS
TGGCTGATAAGAGGCATCGT+CGG 0.580551 1.2:-67379730 MS.gene005686:CDS
CTTGGGGAGTAAATAAGCTG+AGG 0.580784 1.2:-67379514 MS.gene005686:CDS
TATCAGCCATCAAGAACAGA+AGG 0.589403 1.2:+67379744 None:intergenic
TATTTCAGCTCTAAAGCCAA+CGG 0.594762 1.2:-67381970 MS.gene005686:CDS
TGCTTGTTCCATACTTCACT+AGG 0.596244 1.2:-67381206 MS.gene005686:intron
AAAGAAAGTAGCCAGGGAGA+GGG 0.598067 1.2:-67379804 MS.gene005686:CDS
GACCTTAGACGCACAAAAGG+GGG 0.599512 1.2:+67379574 None:intergenic
CAAGAATGCCATCAAAAGAG+TGG 0.606435 1.2:-67382022 MS.gene005686:CDS
CGTCTAAGGTCTTCAACACA+GGG 0.609157 1.2:-67379562 MS.gene005686:CDS
TCTGCAAACTAGCTTGCGGA+AGG 0.611580 1.2:-67379598 MS.gene005686:CDS
TTGGGGAGTAAATAAGCTGA+GGG 0.614442 1.2:-67379513 MS.gene005686:CDS
AAAGATGGGATTGAAAGATG+AGG 0.631344 1.2:-67379459 MS.gene005686:CDS
GTGATCTGCAAACTAGCTTG+CGG 0.634286 1.2:-67379602 MS.gene005686:CDS
AGACCTTAGACGCACAAAAG+GGG 0.635020 1.2:+67379573 None:intergenic
TAGAGTGAATATCCAATGCG+GGG 0.642303 1.2:+67380308 None:intergenic
CTGCAAACTAGCTTGCGGAA+GGG 0.661355 1.2:-67379597 MS.gene005686:CDS
TCACAGATAGCACGACAGCA+AGG 0.673650 1.2:+67379621 None:intergenic
TGTAGAGTGAATATCCAATG+CGG 0.714818 1.2:+67380306 None:intergenic
AGAGTGAATATCCAATGCGG+GGG 0.722783 1.2:+67380309 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATATTTATAATTAAATC+CGG + chr1.2:67380590-67380609 None:intergenic 10.0%
!!! TGAATTTTTTTTTTATGATA+GGG - chr1.2:67379503-67379522 MS.gene005686:CDS 10.0%
!!! TTGAATTTTTTTTTTATGAT+AGG - chr1.2:67379502-67379521 MS.gene005686:CDS 10.0%
!! AAAATGCATAAAATTAAAGT+CGG + chr1.2:67381551-67381570 None:intergenic 15.0%
!! AAAATTAATGTTCATCTAAA+TGG - chr1.2:67380304-67380323 MS.gene005686:CDS 15.0%
!! ATAATAGAAAACAATATTCA+GGG - chr1.2:67380523-67380542 MS.gene005686:intron 15.0%
!! TATACACAAATTCAATAAAT+TGG + chr1.2:67379716-67379735 None:intergenic 15.0%
!!! ATATTTTGATTATACAATGT+TGG - chr1.2:67379743-67379762 MS.gene005686:CDS 15.0%
!! AAATGCATAAAATTAAAGTC+GGG + chr1.2:67381550-67381569 None:intergenic 20.0%
!! CAAGAATAGAATATTGAATA+TGG + chr1.2:67381224-67381243 None:intergenic 20.0%
!! CATAATAGAAAACAATATTC+AGG - chr1.2:67380522-67380541 MS.gene005686:intron 20.0%
!! CTCATAAAAAATATACAACA+TGG + chr1.2:67379836-67379855 None:intergenic 20.0%
!! GTTAAAGTAGTAAAAATCAA+TGG - chr1.2:67381088-67381107 MS.gene005686:intron 20.0%
!! TGTGATCAATTGAATTTAAA+CGG + chr1.2:67380474-67380493 None:intergenic 20.0%
!! TTTATCGAATTTATCTAACT+AGG - chr1.2:67381013-67381032 MS.gene005686:intron 20.0%
!!! AACATTAATTTTTGAGCTAA+GGG + chr1.2:67380296-67380315 None:intergenic 20.0%
!!! CACATTTAAAAATGCATTTT+TGG + chr1.2:67380632-67380651 None:intergenic 20.0%
!!! TAAATTTGTCGTACATATTT+TGG - chr1.2:67380805-67380824 MS.gene005686:intron 20.0%
!!! TAACATCAAATTTAAAACCT+TGG - chr1.2:67380606-67380625 MS.gene005686:intron 20.0%
!!! TACTAATAATTTTACCTTCA+TGG + chr1.2:67379431-67379450 None:intergenic 20.0%
!!! TATCTAATAATATCTGGTTT+TGG + chr1.2:67379557-67379576 None:intergenic 20.0%
! AGATGTAGAAATTCCTATTT+GGG + chr1.2:67380240-67380259 None:intergenic 25.0%
! ATAGCTGTAAATTTGATCAT+TGG - chr1.2:67380915-67380934 MS.gene005686:intron 25.0%
! CTCTGATATCTAATAATATC+TGG + chr1.2:67379563-67379582 None:intergenic 25.0%
! GAATATTACTATATCAGTCA+TGG - chr1.2:67379886-67379905 MS.gene005686:intron 25.0%
! GATGAATTTAACCTAAGATT+TGG + chr1.2:67379587-67379606 None:intergenic 25.0%
! TAGCTGTAAATTTGATCATT+GGG - chr1.2:67380916-67380935 MS.gene005686:intron 25.0%
! TCTTGAAAAAAATCAAGCAA+AGG + chr1.2:67380268-67380287 None:intergenic 25.0%
! TTTCTCTTAAAATTTGAGCT+AGG + chr1.2:67381605-67381624 None:intergenic 25.0%
!! AAGTAATGTAACTTACCTTT+TGG - chr1.2:67380009-67380028 MS.gene005686:intron 25.0%
!! AATATGTTTTCCATGACAAT+TGG - chr1.2:67381382-67381401 MS.gene005686:CDS 25.0%
!! ACTTATGTTGCTAAATCTTT+TGG - chr1.2:67379944-67379963 MS.gene005686:intron 25.0%
!! TATTCTACCTACCTTTTTAA+AGG + chr1.2:67381366-67381385 None:intergenic 25.0%
!!! AGGGAATCAAGTTTTATTTT+TGG - chr1.2:67381638-67381657 MS.gene005686:intron 25.0%
!!! GAACATTAATTTTTGAGCTA+AGG + chr1.2:67380297-67380316 None:intergenic 25.0%
!!! GGGAATCAAGTTTTATTTTT+GGG - chr1.2:67381639-67381658 MS.gene005686:intron 25.0%
!!! TTCTTTTTCTTTTTTCGTGA+TGG + chr1.2:67381819-67381838 None:intergenic 25.0%
AAAAGAAAAAGAAAGTAGCC+AGG - chr1.2:67381826-67381845 MS.gene005686:intron 30.0%
AAAGAAAAAGAAAGTAGCCA+GGG - chr1.2:67381827-67381846 MS.gene005686:intron 30.0%
AGAGAAATAATCATCAGATG+AGG - chr1.2:67381618-67381637 MS.gene005686:intron 30.0%
AGAGAATGCAATGATATTTG+AGG + chr1.2:67380211-67380230 None:intergenic 30.0%
AGGCAACAAATACAAAAATC+AGG - chr1.2:67381033-67381052 MS.gene005686:intron 30.0%
AGTAGAATGCTTGAATTGAA+AGG - chr1.2:67379800-67379819 MS.gene005686:CDS 30.0%
CTGTAAAACAAATGGATCAT+TGG - chr1.2:67381486-67381505 MS.gene005686:CDS 30.0%
GAGAAATAATCATCAGATGA+GGG - chr1.2:67381619-67381638 MS.gene005686:intron 30.0%
GAGATGTAGAAATTCCTATT+TGG + chr1.2:67380241-67380260 None:intergenic 30.0%
GATTCAGAAACTTACTTTGA+TGG - chr1.2:67379375-67379394 MS.gene005686:CDS 30.0%
GCTGAATATAAATGAAGTAG+AGG - chr1.2:67381293-67381312 MS.gene005686:CDS 30.0%
TACGCAAAATATGCTGTTAT+AGG - chr1.2:67381424-67381443 MS.gene005686:CDS 30.0%
TCAAGCATTCTACTAATACA+TGG + chr1.2:67379794-67379813 None:intergenic 30.0%
TCTATCCTCTGTAAAACAAA+TGG - chr1.2:67381478-67381497 MS.gene005686:CDS 30.0%
TGAGGAAATGATTCAAAAAG+TGG - chr1.2:67382196-67382215 MS.gene005686:intron 30.0%
TTTGTTTCCTTCATATGATG+TGG - chr1.2:67381568-67381587 MS.gene005686:CDS 30.0%
! ACTTTTGCTGAAAATCTACA+TGG - chr1.2:67381266-67381285 MS.gene005686:CDS 30.0%
! ATGTTAAAGCTTTATCATGC+AGG + chr1.2:67380696-67380715 None:intergenic 30.0%
! ATTTTGCGTATGTCAAATTC+AGG + chr1.2:67381414-67381433 None:intergenic 30.0%
!! GAGAGCTTTTACTCTAAAAA+TGG - chr1.2:67381512-67381531 MS.gene005686:CDS 30.0%
!! TACCTACCTTTTTAAAGGAA+CGG + chr1.2:67381361-67381380 None:intergenic 30.0%
!!! ATGATCCATTTGTTTTACAG+AGG + chr1.2:67381486-67381505 None:intergenic 30.0%
!!! TAAAAATGCATTTTTGGCCA+AGG + chr1.2:67380626-67380645 None:intergenic 30.0%
!!! TTTCTTTTTTCGTGATGGAT+TGG + chr1.2:67381814-67381833 None:intergenic 30.0%
AAAACAGAGTCACATGCATA+TGG + chr1.2:67380969-67380988 None:intergenic 35.0%
AAACAGAGTCACATGCATAT+GGG + chr1.2:67380968-67380987 None:intergenic 35.0%
AAAGATGGGATTGAAAGATG+AGG - chr1.2:67382178-67382197 MS.gene005686:intron 35.0%
AAATGGTCAAGTCACGTTTA+TGG - chr1.2:67380321-67380340 MS.gene005686:CDS 35.0%
AACAATATTCAGGGACTTCT+TGG - chr1.2:67380532-67380551 MS.gene005686:intron 35.0%
AATGGTCAAGTCACGTTTAT+GGG - chr1.2:67380322-67380341 MS.gene005686:CDS 35.0%
ACTCAATCCACATCATATGA+AGG + chr1.2:67381578-67381597 None:intergenic 35.0%
ATCTCAAGAATATGCCATGA+AGG - chr1.2:67379414-67379433 MS.gene005686:CDS 35.0%
ATGATAGGGTTGTCAACAAT+TGG - chr1.2:67379517-67379536 MS.gene005686:CDS 35.0%
ATTCTCTCATCTACCCAAAT+AGG - chr1.2:67380224-67380243 MS.gene005686:intron 35.0%
CAAATACTGTGATCACTGTA+TGG + chr1.2:67381729-67381748 None:intergenic 35.0%
CACATGAAATCCTTCTGTTT+GGG - chr1.2:67380490-67380509 MS.gene005686:intron 35.0%
GATATCAGAGTCCAAATCTT+AGG - chr1.2:67379573-67379592 MS.gene005686:CDS 35.0%
GTCAATCTATTGATCAACAC+CGG - chr1.2:67380568-67380587 MS.gene005686:intron 35.0%
GTTAACATAAGCAAGAATGC+TGG - chr1.2:67382155-67382174 MS.gene005686:intron 35.0%
GTTCTTTCACTGAAGAAATC+AGG - chr1.2:67381939-67381958 MS.gene005686:intron 35.0%
TCACATGAAATCCTTCTGTT+TGG - chr1.2:67380489-67380508 MS.gene005686:intron 35.0%
TCACTAGGTATGTACATATG+TGG - chr1.2:67380446-67380465 MS.gene005686:intron 35.0%
TGTACATACCTAGTGAAGTA+TGG + chr1.2:67380442-67380461 None:intergenic 35.0%
TGTAGAGTGAATATCCAATG+CGG + chr1.2:67381334-67381353 None:intergenic 35.0%
TTCATGGCATATTCTTGAGA+TGG + chr1.2:67379415-67379434 None:intergenic 35.0%
! CATATGATGTGGATTGAGTT+AGG - chr1.2:67381579-67381598 MS.gene005686:CDS 35.0%
! GAATTGAACCACTCTTTTGA+TGG + chr1.2:67379626-67379645 None:intergenic 35.0%
!! CTGCAAAGTTTTATCATGCT+CGG + chr1.2:67380092-67380111 None:intergenic 35.0%
!! TATTTCAGCTCTAAAGCCAA+CGG - chr1.2:67379667-67379686 MS.gene005686:CDS 35.0%
AAGACCTTAGACGCACAAAA+GGG + chr1.2:67382068-67382087 None:intergenic 40.0%
AAGCAAGAATGCTGGAAAGA+TGG - chr1.2:67382163-67382182 MS.gene005686:intron 40.0%
AGAGAATTGTGTAAGGAGCT+CGG + chr1.2:67381976-67381995 None:intergenic 40.0%
AGCAAGAATGCTGGAAAGAT+GGG - chr1.2:67382164-67382183 MS.gene005686:intron 40.0%
AGTGTATTGTCTGATACTGG+AGG - chr1.2:67380662-67380681 MS.gene005686:intron 40.0%
CAAGAATGCCATCAAAAGAG+TGG - chr1.2:67379615-67379634 MS.gene005686:CDS 40.0%
CCAACGGTTATTGATGTACA+AGG - chr1.2:67379683-67379702 MS.gene005686:CDS 40.0%
CCTTGTACATCAATAACCGT+TGG + chr1.2:67379686-67379705 None:intergenic 40.0%
GACAAAGTAACCCAAACAGA+AGG + chr1.2:67380503-67380522 None:intergenic 40.0%
GACAATCCTCTACATATCAC+CGG + chr1.2:67379914-67379933 None:intergenic 40.0%
GCACATGAGACTATCAACAA+TGG + chr1.2:67381704-67381723 None:intergenic 40.0%
GCGTAACTTCAAAGGTTTGT+CGG + chr1.2:67381158-67381177 None:intergenic 40.0%
GTAGAGTGAATATCCAATGC+GGG + chr1.2:67381333-67381352 None:intergenic 40.0%
GTATCAAAACGCACACATAC+AGG - chr1.2:67380394-67380413 MS.gene005686:intron 40.0%
GTTTGTCGGAAACTTAGTTC+AGG + chr1.2:67381144-67381163 None:intergenic 40.0%
TACTATATCAGTCATGGTGC+CGG - chr1.2:67379892-67379911 MS.gene005686:intron 40.0%
TAGAGTGAATATCCAATGCG+GGG + chr1.2:67381332-67381351 None:intergenic 40.0%
TATCAGCCATCAAGAACAGA+AGG + chr1.2:67381896-67381915 None:intergenic 40.0%
TCGAGTGTATTGTCTGATAC+TGG - chr1.2:67380659-67380678 MS.gene005686:intron 40.0%
TGCTGAAAATCTACATGGAC+AGG - chr1.2:67381271-67381290 MS.gene005686:CDS 40.0%
TGCTTGTTCCATACTTCACT+AGG - chr1.2:67380431-67380450 MS.gene005686:intron 40.0%
TTCACTGAAGAAATCAGGTC+CGG - chr1.2:67381944-67381963 MS.gene005686:intron 40.0%
TTGCGTATGTCAAATTCAGG+AGG + chr1.2:67381411-67381430 None:intergenic 40.0%
TTGGGGAGTAAATAAGCTGA+GGG - chr1.2:67382124-67382143 MS.gene005686:intron 40.0%
! AAGCTGAAAAGTACAGTGCT+TGG + chr1.2:67380049-67380068 None:intergenic 40.0%
! AGCACTGTACTTTTCAGCTT+TGG - chr1.2:67380049-67380068 MS.gene005686:intron 40.0%
! CACTGACCGTTCCTTTAAAA+AGG - chr1.2:67381352-67381371 MS.gene005686:CDS 40.0%
! CTTACTTTGATGGCTTCTCA+TGG - chr1.2:67379385-67379404 MS.gene005686:CDS 40.0%
! GACCGTTCCTTTAAAAAGGT+AGG - chr1.2:67381356-67381375 MS.gene005686:CDS 40.0%
! GCTTTGTTGTCTGACTTGAT+GGG - chr1.2:67380160-67380179 MS.gene005686:intron 40.0%
! TGTTCTTGATGGCTGATAAG+AGG - chr1.2:67381898-67381917 MS.gene005686:intron 40.0%
!! AGCTTTGTTGTCTGACTTGA+TGG - chr1.2:67380159-67380178 MS.gene005686:intron 40.0%
!! GGATTGGCTTTGACTTTCTT+CGG + chr1.2:67381798-67381817 None:intergenic 40.0%
!! TGATGGGTTTGCAACTATCA+GGG - chr1.2:67380176-67380195 MS.gene005686:intron 40.0%
!! TGGGCTTATTTGGCTTTCTT+GGG - chr1.2:67382106-67382125 MS.gene005686:CDS 40.0%
!! TTGATGGGTTTGCAACTATC+AGG - chr1.2:67380175-67380194 MS.gene005686:intron 40.0%
!! TTGGGCTTATTTGGCTTTCT+TGG - chr1.2:67382105-67382124 MS.gene005686:CDS 40.0%
!!! TCTGGTTTTGGTGCATAACA+AGG + chr1.2:67379545-67379564 None:intergenic 40.0%
AAAAGAAAGTAGCCAGGGAG+AGG - chr1.2:67381832-67381851 MS.gene005686:intron 45.0%
AAAGAAAGTAGCCAGGGAGA+GGG - chr1.2:67381833-67381852 MS.gene005686:intron 45.0%
AAGGTCTTCAACACAGGGTT+GGG - chr1.2:67382080-67382099 MS.gene005686:CDS 45.0%
AGACCTTAGACGCACAAAAG+GGG + chr1.2:67382067-67382086 None:intergenic 45.0%
AGAGTGAATATCCAATGCGG+GGG + chr1.2:67381331-67381350 None:intergenic 45.0%
AGTCACATGCATATGGGATC+AGG + chr1.2:67380962-67380981 None:intergenic 45.0%
CATGTTACTCTTGTCTTGCC+AGG - chr1.2:67380850-67380869 MS.gene005686:intron 45.0%
CTCCTTACACAATTCTCTGC+TGG - chr1.2:67381978-67381997 MS.gene005686:CDS 45.0%
CTTGGGGAGTAAATAAGCTG+AGG - chr1.2:67382123-67382142 MS.gene005686:intron 45.0%
GAAGACCTTAGACGCACAAA+AGG + chr1.2:67382069-67382088 None:intergenic 45.0%
GACAAGTAGCTGCAACCAAA+AGG + chr1.2:67380027-67380046 None:intergenic 45.0%
GAGAATTGTGTAAGGAGCTC+GGG + chr1.2:67381975-67381994 None:intergenic 45.0%
GCACGTATGCGTAACTTCAA+AGG + chr1.2:67381166-67381185 None:intergenic 45.0%
GGACTCACTATGCAAGAACA+AGG + chr1.2:67381118-67381137 None:intergenic 45.0%
TAAGGTCTTCAACACAGGGT+TGG - chr1.2:67382079-67382098 MS.gene005686:CDS 45.0%
TCACTATGCAAGAACAAGGC+AGG + chr1.2:67381114-67381133 None:intergenic 45.0%
TCGGAAACTTAGTTCAGGCA+GGG + chr1.2:67381139-67381158 None:intergenic 45.0%
TGAGAGCCTTCTGTTCTTGA+TGG - chr1.2:67381887-67381906 MS.gene005686:intron 45.0%
TGCCAGCAGAGAATTGTGTA+AGG + chr1.2:67381983-67382002 None:intergenic 45.0%
! CGTCTAAGGTCTTCAACACA+GGG - chr1.2:67382075-67382094 MS.gene005686:CDS 45.0%
! GTGATCTGCAAACTAGCTTG+CGG - chr1.2:67382035-67382054 MS.gene005686:CDS 45.0%
! TCAACACAGGGTTGGGTTTT+GGG - chr1.2:67382087-67382106 MS.gene005686:CDS 45.0%
! TTCAACACAGGGTTGGGTTT+TGG - chr1.2:67382086-67382105 MS.gene005686:CDS 45.0%
!! CAGGAGTGCTGTGTTAGTTT+GGG - chr1.2:67380869-67380888 MS.gene005686:intron 45.0%
!! GGGCTTATTTGGCTTTCTTG+GGG - chr1.2:67382107-67382126 MS.gene005686:CDS 45.0%
!!! GGTTGGGTTTTGGGCTTATT+TGG - chr1.2:67382096-67382115 MS.gene005686:CDS 45.0%
ACTGCTGAAGCAGGTGTAGA+TGG - chr1.2:67380127-67380146 MS.gene005686:intron 50.0%
CAATTCTCTGCTGGCATTGC+TGG - chr1.2:67381987-67382006 MS.gene005686:CDS 50.0%
CAGGAGGAAGCCAATTGTCA+TGG + chr1.2:67381395-67381414 None:intergenic 50.0%
CCAAACTAACACAGCACTCC+TGG + chr1.2:67380871-67380890 None:intergenic 50.0%
GACCTTAGACGCACAAAAGG+GGG + chr1.2:67382066-67382085 None:intergenic 50.0%
GTCGGAAACTTAGTTCAGGC+AGG + chr1.2:67381140-67381159 None:intergenic 50.0%
TCACAGATAGCACGACAGCA+AGG + chr1.2:67382019-67382038 None:intergenic 50.0%
TGCTAGTAGTGGCAGTGCTA+AGG - chr1.2:67382258-67382277 MS.gene005686:CDS 50.0%
! ACATCTGAGACTGCTGAAGC+AGG - chr1.2:67380118-67380137 MS.gene005686:intron 50.0%
! CTGCAAACTAGCTTGCGGAA+GGG - chr1.2:67382040-67382059 MS.gene005686:CDS 50.0%
! GCGTCTAAGGTCTTCAACAC+AGG - chr1.2:67382074-67382093 MS.gene005686:CDS 50.0%
! TCTGCAAACTAGCTTGCGGA+AGG - chr1.2:67382039-67382058 MS.gene005686:CDS 50.0%
!! ATGGTGCCGGTGATATGTAG+AGG - chr1.2:67379905-67379924 MS.gene005686:intron 50.0%
!! CCAGGAGTGCTGTGTTAGTT+TGG - chr1.2:67380868-67380887 MS.gene005686:intron 50.0%
!! TGGCTGATAAGAGGCATCGT+CGG - chr1.2:67381907-67381926 MS.gene005686:intron 50.0%
AGCTGCAGCGTTGCTAGTAG+TGG - chr1.2:67382247-67382266 MS.gene005686:CDS 55.0%
CAATGCGGGGGAAATGCTTC+TGG + chr1.2:67381319-67381338 None:intergenic 55.0%
CTTGAACAGATCCCTCTCCC+TGG + chr1.2:67381847-67381866 None:intergenic 55.0%
TCTGCTGGCATTGCTGGTAC+TGG - chr1.2:67381993-67382012 MS.gene005686:CDS 55.0%
!! CAGAAGCATTTCCCCCGCAT+TGG - chr1.2:67381317-67381336 MS.gene005686:CDS 55.0%
!! GGCTGATAAGAGGCATCGTC+GGG - chr1.2:67381908-67381927 MS.gene005686:intron 55.0%
AGCGCGCGAACAGTGAGCAA+CGG - chr1.2:67380770-67380789 MS.gene005686:intron 60.0%
GCTCACTGTTCGCGCGCTTA+TGG + chr1.2:67380768-67380787 None:intergenic 60.0%
GTAAGGAGCTCGGGAAGCTC+CGG + chr1.2:67381966-67381985 None:intergenic 60.0%
! GGCCCCCTTTTGTGCGTCTA+AGG - chr1.2:67382061-67382080 MS.gene005686:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 67379370 67382289 67379370 ID=MS.gene005686
chr1.2 mRNA 67379370 67382289 67379370 ID=MS.gene005686.t1;Parent=MS.gene005686
chr1.2 exon 67382224 67382289 67382224 ID=MS.gene005686.t1.exon1;Parent=MS.gene005686.t1
chr1.2 CDS 67382224 67382289 67382224 ID=cds.MS.gene005686.t1;Parent=MS.gene005686.t1
chr1.2 exon 67381955 67382135 67381955 ID=MS.gene005686.t1.exon2;Parent=MS.gene005686.t1
chr1.2 CDS 67381955 67382135 67381955 ID=cds.MS.gene005686.t1;Parent=MS.gene005686.t1
chr1.2 exon 67381207 67381621 67381207 ID=MS.gene005686.t1.exon3;Parent=MS.gene005686.t1
chr1.2 CDS 67381207 67381621 67381207 ID=cds.MS.gene005686.t1;Parent=MS.gene005686.t1
chr1.2 exon 67380286 67380366 67380286 ID=MS.gene005686.t1.exon4;Parent=MS.gene005686.t1
chr1.2 CDS 67380286 67380366 67380286 ID=cds.MS.gene005686.t1;Parent=MS.gene005686.t1
chr1.2 exon 67379370 67379865 67379370 ID=MS.gene005686.t1.exon5;Parent=MS.gene005686.t1
chr1.2 CDS 67379370 67379865 67379370 ID=cds.MS.gene005686.t1;Parent=MS.gene005686.t1
Gene Sequence

>MS.gene005686

ATGATGATTCAGAAACTTACTTTGATGGCTTCTCATGGATTTCCATCTCAAGAATATGCCATGAAGGTAAAATTATTAGTATTGTTTATGATTTTATTTTTGTCACTGAAGTATTTTTTGTTTTAATTTTGATTGAATTTTTTTTTTATGATAGGGTTGTCAACAATTGGTGCCTTGTTATGCACCAAAACCAGATATTATTAGATATCAGAGTCCAAATCTTAGGTTAAATTCATCTGAAGAATCAAGAATGCCATCAAAAGAGTGGTTCAATTCAAATCAGTTTGTTGATTTTGATATTTCAGCTCTAAAGCCAACGGTTATTGATGTACAAGGTGTGTAGCCAATTTATTGAATTTGTGTATATGATGCAATATTTTGATTATACAATGTTGGTATTTTTAGTTTATTTGTTTTCTTTCCATGTATTAGTAGAATGCTTGAATTGAAAGGTACTTTTTTTCCATGTTGTATATTTTTTATGAGCAATGTGATAGTAACTGAAATTATTCTTAAGAATATTACTATATCAGTCATGGTGCCGGTGATATGTAGAGGATTGTCTAAGATGATGACTTATGTTGCTAAATCTTTTGGTTCAATATTAAGTATTGTAACTTATCTTATGTGTCTTACTCTAAGTAATGTAACTTACCTTTTGGTTGCAGCTACTTGTCCAAGCACTGTACTTTTCAGCTTTGGCATCATTGATCAATGCACCGAGCATGATAAAACTTTGCAGTGTATCACATCTGAGACTGCTGAAGCAGGTGTAGATGGTGTTCGTAAAGCTTTGTTGTCTGACTTGATGGGTTTGCAACTATCAGGGATTAATATGCCTCAAATATCATTGCATTCTCTCATCTACCCAAATAGGAATTTCTACATCTCGAAGCCTTTGCTTGATTTTTTTCAAGATTCAGCCCTTAGCTCAAAAATTAATGTTCATCTAAATGGTCAAGTCACGTTTATGGGTACTGAAATTGAGATGAAAGATTTTCTTGCTATTGTTGCTGAGTCATACGTATCAAAACGCACACATACAGGCGAAAAACAGTCTATGCTTGTTCCATACTTCACTAGGTATGTACATATGTGGAACCGTTTAAATTCAATTGATCACATGAAATCCTTCTGTTTGGGTTACTTTGTCATAATAGAAAACAATATTCAGGGACTTCTTGGTTAAGTTATGACTGTCAATCTATTGATCAACACCGGATTTAATTATAAATATAACATCAAATTTAAAACCTTGGCCAAAAATGCATTTTTAAATGTGTTTGTTTTCGAGTGTATTGTCTGATACTGGAGGCTTTACAACCTGCATGATAAAGCTTTAACATTGTTGACTCTACTATATTGCATTCATGACAAGTAGTTGAAGAATTGTATCCATAAGCGCGCGAACAGTGAGCAACGGTGCTTGTTCTAATAAATTTGTCGTACATATTTTGGTCACTTTGAAAAATTACTAGCTCATGTTACTCTTGTCTTGCCAGGAGTGCTGTGTTAGTTTGGGTCATTGTGATACTGTCATTTAAGATAGCTGTAAATTTGATCATTGGGACAACAGATTTCTTTTTTCTCCTGATCCCATATGCATGTGACTCTGTTTTTTCTTATTTGAATTTTTATATTAATTTATCGAATTTATCTAACTAGGCAACAAATACAAAAATCAGGATGCATCTATGATAAGTTAAATACTACTATAAGTTAAAGTAGTAAAAATCAATGGCCTGCCTTGTTCTTGCATAGTGAGTCCCTGCCTGAACTAAGTTTCCGACAAACCTTTGAAGTTACGCATACGTGCTTCAGTTATAGATAATACACATACTATCTGATTATCCATATTCAATATTCTATTCTTGTTAGTTATGTATCAATCAACTGACTTTTGCTGAAAATCTACATGGACAGGCTGAATATAAATGAAGTAGAGGCCAGAAGCATTTCCCCCGCATTGGATATTCACTCTACACTGACCGTTCCTTTAAAAAGGTAGGTAGAATATGTTTTCCATGACAATTGGCTTCCTCCTGAATTTGACATACGCAAAATATGCTGTTATAGGAGTCATTTTTATTCAAACTGTTCATAATCATTCTATCCTCTGTAAAACAAATGGATCATTGGTCTGAGAGCTTTTACTCTAAAAATGGTTTAATTTTATGCCCGACTTTAATTTTATGCATTTTGTTTCCTTCATATGATGTGGATTGAGTTAGGCCTAGCTCAAATTTTAAGAGAAATAATCATCAGATGAGGGAATCAAGTTTTATTTTTGGGCTGTATTATTGATTTCATTTATGTTAACTGTTTCTGTTGCCATTGTTGATAGTCTCATGTGCTGCCATACAGTGATCACAGTATTTGCTCAAGAAATATATTAATATTTAATGTATTTCTCTTGCTTGACAGTCCGAAGAAAGTCAAAGCCAATCCATCACGAAAAAAGAAAAAGAAAGTAGCCAGGGAGAGGGATCTGTTCAAGAAGAACTACATTCATGCATGTGAGAGCCTTCTGTTCTTGATGGCTGATAAGAGGCATCGTCGGGAAACAGCAGTTCTTTCACTGAAGAAATCAGGTCCGGAGCTTCCCGAGCTCCTTACACAATTCTCTGCTGGCATTGCTGGTACTGGCCTTGCTGTCGTGCTATCTGTGATCTGCAAACTAGCTTGCGGAAGGGCCCCCTTTTGTGCGTCTAAGGTCTTCAACACAGGGTTGGGTTTTGGGCTTATTTGGCTTTCTTGGGGAGTAAATAAGCTGAGGGATACAATTGTTAACATAAGCAAGAATGCTGGAAAGATGGGATTGAAAGATGAGGAAATGATTCAAAAAGTGGATAAGAGTCTAAAAGAAGTTTACTTTAGAGCTGCAGCGTTGCTAGTAGTGGCAGTGCTAAGGCTTGCTTGA
Protein sequence

>MS.gene005686.t1

MMIQKLTLMASHGFPSQEYAMKGCQQLVPCYAPKPDIIRYQSPNLRLNSSEESRMPSKEWFNSNQFVDFDISALKPTVIDVQATCPSTVLFSFGIIDQCTEHDKTLQCITSETAEAGVDGVRKALLSDLMGLQLSGINMPQISLHSLIYPNRNFYISKPLLDFFQDSALSSKINVHLNGQVTFMGTEIEMKDFLAIVAESYVSKRTHTGEKQSMLVPYFTRLNINEVEARSISPALDIHSTLTVPLKSPKKVKANPSRKKKKKVARERDLFKKNYIHACESLLFLMADKRHRRETAVLSLKKSGPELPELLTQFSAGIAGTGLAVVLSVICKLACGRAPFCASKVFNTGLGFGLIWLSWGVNKLRDTIVNISKNAGKMGLKDEEMIQKVDKSLKEVYFRAAALLVVAVLRLA