Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006242.t1 | AFK38248.1 | 97.3 | 184 | 5 | 0 | 1 | 184 | 7 | 190 | 1.60E-69 | 272.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006242.t1 | I3SDA6 | 97.3 | 184 | 5 | 0 | 1 | 184 | 7 | 190 | 1.2e-69 | 272.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006242 | MS.gene49145 | PPI |
| MS.gene006242 | MS.gene60604 | PPI |
| MS.gene006242 | MS.gene061135 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006242.t1 | MTR_1g100090 | 97.794 | 136 | 3 | 0 | 1 | 136 | 7 | 142 | 3.56e-95 | 273 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006242.t1 | AT4G25670 | 54.054 | 185 | 81 | 3 | 1 | 184 | 1 | 182 | 3.32e-50 | 160 |
| MS.gene006242.t1 | AT4G25670 | 54.054 | 185 | 81 | 3 | 1 | 184 | 1 | 182 | 3.32e-50 | 160 |
| MS.gene006242.t1 | AT4G25690 | 51.795 | 195 | 73 | 5 | 1 | 184 | 1 | 185 | 1.09e-49 | 159 |
| MS.gene006242.t1 | AT4G25690 | 51.795 | 195 | 73 | 5 | 1 | 184 | 1 | 185 | 1.09e-49 | 159 |
| MS.gene006242.t1 | AT5G52550 | 51.099 | 182 | 66 | 6 | 17 | 184 | 182 | 354 | 2.05e-44 | 150 |
| MS.gene006242.t1 | AT5G52550 | 74.576 | 59 | 15 | 0 | 17 | 75 | 79 | 137 | 9.99e-18 | 79.7 |
Find 38 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAACATGGCATGTAATAA+AGG | 0.286451 | 1.1:+71884560 | MS.gene006242:CDS |
| AGATAGACCACTTGCCGTTT+TGG | 0.291490 | 1.1:-71884940 | None:intergenic |
| TAATAATCTTGATTTCTTCA+TGG | 0.361966 | 1.1:+71884862 | MS.gene006242:CDS |
| GCTTGCTTTCCTAATCTAGA+TGG | 0.392945 | 1.1:+71884896 | MS.gene006242:CDS |
| AGACTCAGATGATTCATATA+AGG | 0.397214 | 1.1:+71884811 | MS.gene006242:CDS |
| TGCTGCCATCATATCGGAAC+TGG | 0.404529 | 1.1:+71884691 | MS.gene006242:CDS |
| AATAATCTTGATTTCTTCAT+GGG | 0.406348 | 1.1:+71884863 | MS.gene006242:CDS |
| TACTTCTGCTGCCATCATAT+CGG | 0.438376 | 1.1:+71884685 | MS.gene006242:CDS |
| ATAACATGGCATGTAATAAA+GGG | 0.468465 | 1.1:+71884561 | MS.gene006242:CDS |
| CATGTGCTACCATCTAGATT+AGG | 0.471484 | 1.1:-71884905 | None:intergenic |
| CACGAACCACTTTACGAAGC+AGG | 0.483308 | 1.1:+71884989 | MS.gene006242:CDS |
| TAAGGTTGAGTGCAAGGCCT+GGG | 0.484400 | 1.1:+71884829 | MS.gene006242:CDS |
| AGATCAACAGAGAATCTTGT+AGG | 0.486420 | 1.1:-71885019 | None:intergenic |
| TTATAGCAGCACAAGCAGTT+AGG | 0.486837 | 1.1:+71885041 | MS.gene006242:CDS |
| ATCTTGATTTCTTCATGGGT+GGG | 0.489941 | 1.1:+71884867 | MS.gene006242:CDS |
| GAAGCAGGTGAAAGATGAGC+TGG | 0.492593 | 1.1:+71884610 | MS.gene006242:CDS |
| TGGTCCGTCACTTCCCAAAA+CGG | 0.505701 | 1.1:+71884926 | MS.gene006242:CDS |
| AGGATAGGATTCTCTTCTAA+AGG | 0.516327 | 1.1:+71885092 | MS.gene006242:CDS |
| AATCTTGATTTCTTCATGGG+TGG | 0.519569 | 1.1:+71884866 | MS.gene006242:CDS |
| TCCCACCCTGCTTCGTAAAG+TGG | 0.522362 | 1.1:-71884995 | None:intergenic |
| CAGAGAATGCAGATGAGGAT+AGG | 0.524026 | 1.1:+71885077 | MS.gene006242:CDS |
| GGCAAGTGGTCTATCTCGTC+CGG | 0.524358 | 1.1:+71884947 | MS.gene006242:CDS |
| ATAAGGTTGAGTGCAAGGCC+TGG | 0.527429 | 1.1:+71884828 | MS.gene006242:CDS |
| GAGAAAGACAAAGAAGAAGC+AGG | 0.540706 | 1.1:+71884595 | MS.gene006242:CDS |
| TTCATATAAGGTTGAGTGCA+AGG | 0.541798 | 1.1:+71884823 | MS.gene006242:CDS |
| ATTATTATTATAATCATCCC+AGG | 0.551179 | 1.1:-71884846 | None:intergenic |
| GAGTAGAACATGCAGAGCGA+CGG | 0.587047 | 1.1:-71884783 | None:intergenic |
| CATCTGCATTCTCTGCAATG+TGG | 0.593120 | 1.1:-71885069 | None:intergenic |
| ATTAAGTTCAATGGATAACA+TGG | 0.608143 | 1.1:+71884547 | None:intergenic |
| TCACTTCCCAAAACGGCAAG+TGG | 0.610829 | 1.1:+71884933 | MS.gene006242:CDS |
| CATTGCAGAGAATGCAGATG+AGG | 0.616435 | 1.1:+71885072 | MS.gene006242:CDS |
| ACAGCAAAGACTTGATGAAG+AGG | 0.619614 | 1.1:+71884742 | MS.gene006242:CDS |
| ACGAACCACTTTACGAAGCA+GGG | 0.620702 | 1.1:+71884990 | MS.gene006242:CDS |
| TGAAGAGGGTGCTGCAATTG+CGG | 0.628142 | 1.1:+71884757 | MS.gene006242:CDS |
| ACCACTTTACGAAGCAGGGT+GGG | 0.630797 | 1.1:+71884994 | MS.gene006242:CDS |
| CTAATCTAGATGGTAGCACA+TGG | 0.631528 | 1.1:+71884906 | MS.gene006242:CDS |
| CAGCAAAGACTTGATGAAGA+GGG | 0.636235 | 1.1:+71884743 | MS.gene006242:CDS |
| AACCACTTTACGAAGCAGGG+TGG | 0.676621 | 1.1:+71884993 | MS.gene006242:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATCTTGATTTCTTCAT+GGG | + | chr1.1:71884863-71884882 | MS.gene006242:CDS | 20.0% |
| !! | ATTATTATTATAATCATCCC+AGG | - | chr1.1:71884849-71884868 | None:intergenic | 20.0% |
| !! | TAATAATCTTGATTTCTTCA+TGG | + | chr1.1:71884862-71884881 | MS.gene006242:CDS | 20.0% |
| ! | ATAACATGGCATGTAATAAA+GGG | + | chr1.1:71884561-71884580 | MS.gene006242:CDS | 25.0% |
| AAGCTGAAAAGAAAAAGAGA+CGG | + | chr1.1:71884645-71884664 | MS.gene006242:CDS | 30.0% | |
| AGACTCAGATGATTCATATA+AGG | + | chr1.1:71884811-71884830 | MS.gene006242:CDS | 30.0% | |
| GATAACATGGCATGTAATAA+AGG | + | chr1.1:71884560-71884579 | MS.gene006242:CDS | 30.0% | |
| AATCTTGATTTCTTCATGGG+TGG | + | chr1.1:71884866-71884885 | MS.gene006242:CDS | 35.0% | |
| AGATCAACAGAGAATCTTGT+AGG | - | chr1.1:71885022-71885041 | None:intergenic | 35.0% | |
| ATCTTGATTTCTTCATGGGT+GGG | + | chr1.1:71884867-71884886 | MS.gene006242:CDS | 35.0% | |
| TGAAAAGAAAAAGAGACGGT+TGG | + | chr1.1:71884649-71884668 | MS.gene006242:CDS | 35.0% | |
| TTCATATAAGGTTGAGTGCA+AGG | + | chr1.1:71884823-71884842 | MS.gene006242:CDS | 35.0% | |
| ! | TTTTTCCAGTTCCGATATGA+TGG | - | chr1.1:71884699-71884718 | None:intergenic | 35.0% |
| ACAGCAAAGACTTGATGAAG+AGG | + | chr1.1:71884742-71884761 | MS.gene006242:CDS | 40.0% | |
| CAGCAAAGACTTGATGAAGA+GGG | + | chr1.1:71884743-71884762 | MS.gene006242:CDS | 40.0% | |
| CATGTGCTACCATCTAGATT+AGG | - | chr1.1:71884908-71884927 | None:intergenic | 40.0% | |
| CTAATCTAGATGGTAGCACA+TGG | + | chr1.1:71884906-71884925 | MS.gene006242:CDS | 40.0% | |
| GAGAAAGACAAAGAAGAAGC+AGG | + | chr1.1:71884595-71884614 | MS.gene006242:CDS | 40.0% | |
| GCTTGCTTTCCTAATCTAGA+TGG | + | chr1.1:71884896-71884915 | MS.gene006242:CDS | 40.0% | |
| TACTTCTGCTGCCATCATAT+CGG | + | chr1.1:71884685-71884704 | MS.gene006242:CDS | 40.0% | |
| TTATAGCAGCACAAGCAGTT+AGG | + | chr1.1:71885041-71885060 | MS.gene006242:CDS | 40.0% | |
| ! | CACATTGTTTTCGAAAGGTC+CGG | - | chr1.1:71884969-71884988 | None:intergenic | 40.0% |
| ! | TCGTGCACATTGTTTTCGAA+AGG | - | chr1.1:71884974-71884993 | None:intergenic | 40.0% |
| ACGAACCACTTTACGAAGCA+GGG | + | chr1.1:71884990-71885009 | MS.gene006242:CDS | 45.0% | |
| CAGAGAATGCAGATGAGGAT+AGG | + | chr1.1:71885077-71885096 | MS.gene006242:CDS | 45.0% | |
| CATCTGCATTCTCTGCAATG+TGG | - | chr1.1:71885072-71885091 | None:intergenic | 45.0% | |
| CATTGCAGAGAATGCAGATG+AGG | + | chr1.1:71885072-71885091 | MS.gene006242:CDS | 45.0% | |
| !! | AGATAGACCACTTGCCGTTT+TGG | - | chr1.1:71884943-71884962 | None:intergenic | 45.0% |
| !!! | GATAGACCACTTGCCGTTTT+GGG | - | chr1.1:71884942-71884961 | None:intergenic | 45.0% |
| AACCACTTTACGAAGCAGGG+TGG | + | chr1.1:71884993-71885012 | MS.gene006242:CDS | 50.0% | |
| ACCACTTTACGAAGCAGGGT+GGG | + | chr1.1:71884994-71885013 | MS.gene006242:CDS | 50.0% | |
| ATAAGGTTGAGTGCAAGGCC+TGG | + | chr1.1:71884828-71884847 | MS.gene006242:CDS | 50.0% | |
| CACGAACCACTTTACGAAGC+AGG | + | chr1.1:71884989-71885008 | MS.gene006242:CDS | 50.0% | |
| GAAGCAGGTGAAAGATGAGC+TGG | + | chr1.1:71884610-71884629 | MS.gene006242:CDS | 50.0% | |
| GAGACGGTTGGAAAAAGCTC+TGG | + | chr1.1:71884661-71884680 | MS.gene006242:CDS | 50.0% | |
| GAGTAGAACATGCAGAGCGA+CGG | - | chr1.1:71884786-71884805 | None:intergenic | 50.0% | |
| TAAGGTTGAGTGCAAGGCCT+GGG | + | chr1.1:71884829-71884848 | MS.gene006242:CDS | 50.0% | |
| TCACTTCCCAAAACGGCAAG+TGG | + | chr1.1:71884933-71884952 | MS.gene006242:CDS | 50.0% | |
| TGCTGCCATCATATCGGAAC+TGG | + | chr1.1:71884691-71884710 | MS.gene006242:CDS | 50.0% | |
| TGGTCCGTCACTTCCCAAAA+CGG | + | chr1.1:71884926-71884945 | MS.gene006242:CDS | 50.0% | |
| !! | TGAAGAGGGTGCTGCAATTG+CGG | + | chr1.1:71884757-71884776 | MS.gene006242:CDS | 50.0% |
| !!! | CTTGCCGTTTTGGGAAGTGA+CGG | - | chr1.1:71884933-71884952 | None:intergenic | 50.0% |
| GGCAAGTGGTCTATCTCGTC+CGG | + | chr1.1:71884947-71884966 | MS.gene006242:CDS | 55.0% | |
| TCCCACCCTGCTTCGTAAAG+TGG | - | chr1.1:71884998-71885017 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 71884557 | 71885111 | 71884557 | ID=MS.gene006242 |
| chr1.1 | mRNA | 71884557 | 71885111 | 71884557 | ID=MS.gene006242.t1;Parent=MS.gene006242 |
| chr1.1 | exon | 71884557 | 71885111 | 71884557 | ID=MS.gene006242.t1.exon1;Parent=MS.gene006242.t1 |
| chr1.1 | CDS | 71884557 | 71885111 | 71884557 | ID=cds.MS.gene006242.t1;Parent=MS.gene006242.t1 |
| Gene Sequence |
| Protein sequence |
