Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60604.t1 | AFK38248.1 | 97.8 | 184 | 4 | 0 | 1 | 184 | 7 | 190 | 7.30E-70 | 273.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60604.t1 | I3SDA6 | 97.8 | 184 | 4 | 0 | 1 | 184 | 7 | 190 | 5.3e-70 | 273.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60604 | MS.gene061135 | PPI |
| MS.gene006242 | MS.gene60604 | PPI |
| MS.gene60604 | MS.gene49145 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60604.t1 | MTR_1g100090 | 97.794 | 136 | 3 | 0 | 1 | 136 | 7 | 142 | 3.02e-95 | 274 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60604.t1 | AT4G25670 | 54.011 | 187 | 78 | 5 | 1 | 184 | 1 | 182 | 2.15e-50 | 161 |
| MS.gene60604.t1 | AT4G25670 | 54.011 | 187 | 78 | 5 | 1 | 184 | 1 | 182 | 2.15e-50 | 161 |
| MS.gene60604.t1 | AT4G25690 | 51.282 | 195 | 74 | 5 | 1 | 184 | 1 | 185 | 5.46e-49 | 157 |
| MS.gene60604.t1 | AT4G25690 | 51.282 | 195 | 74 | 5 | 1 | 184 | 1 | 185 | 5.46e-49 | 157 |
| MS.gene60604.t1 | AT5G52550 | 51.099 | 182 | 66 | 6 | 17 | 184 | 182 | 354 | 1.99e-44 | 150 |
| MS.gene60604.t1 | AT5G52550 | 74.576 | 59 | 15 | 0 | 17 | 75 | 79 | 137 | 1.04e-17 | 79.7 |
Find 38 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAACATGGCATGTAATAA+AGG | 0.286451 | 1.2:-69544466 | MS.gene60604:CDS |
| AGATAGACCACTTGCCGTTT+TGG | 0.291490 | 1.2:+69544086 | None:intergenic |
| TAATAATCTTGATTTCTTCA+TGG | 0.361966 | 1.2:-69544164 | MS.gene60604:CDS |
| GCTTGCTTTCCTAATCTAGA+TGG | 0.392945 | 1.2:-69544130 | MS.gene60604:CDS |
| AGACTCAGATGATTCATATA+AGG | 0.397214 | 1.2:-69544215 | MS.gene60604:CDS |
| TGCTGCCATCATATCGGAAC+TGG | 0.404529 | 1.2:-69544335 | MS.gene60604:CDS |
| AATAATCTTGATTTCTTCAT+GGG | 0.406348 | 1.2:-69544163 | MS.gene60604:CDS |
| AGGATAGGATTCTCTTCTAA+TGG | 0.433534 | 1.2:-69543934 | None:intergenic |
| TACTTCTGCTGCCATCATAT+CGG | 0.438376 | 1.2:-69544341 | MS.gene60604:CDS |
| ATAACATGGCATGTAATAAA+GGG | 0.468465 | 1.2:-69544465 | MS.gene60604:CDS |
| CATGTGCTACCATCTAGATT+AGG | 0.471484 | 1.2:+69544121 | None:intergenic |
| TAAGGTTGAGTGCAAGGCCT+GGG | 0.484400 | 1.2:-69544197 | MS.gene60604:CDS |
| CACGAACCACTTTACGAAGC+AGG | 0.484453 | 1.2:-69544037 | MS.gene60604:CDS |
| AGATCAACAGAGAATCTTGT+AGG | 0.486420 | 1.2:+69544007 | None:intergenic |
| TTATAGCAGCACAAGCAGTT+AGG | 0.486837 | 1.2:-69543985 | MS.gene60604:CDS |
| ATCTTGATTTCTTCATGGGT+GGG | 0.489941 | 1.2:-69544159 | MS.gene60604:CDS |
| GAAGCAGGTGAAAGATGAGC+TGG | 0.492593 | 1.2:-69544416 | MS.gene60604:CDS |
| TGGTCCGTCACTTCCCAAAA+CGG | 0.505701 | 1.2:-69544100 | MS.gene60604:CDS |
| AATCTTGATTTCTTCATGGG+TGG | 0.519569 | 1.2:-69544160 | MS.gene60604:CDS |
| TCCCACCCTGCTTCGTAAAG+TGG | 0.522362 | 1.2:+69544031 | None:intergenic |
| CAGAGAATGCAGATGAGGAT+AGG | 0.524026 | 1.2:-69543949 | MS.gene60604:CDS |
| GGCAAGTGGTCTATCTCGTC+CGG | 0.524358 | 1.2:-69544079 | MS.gene60604:CDS |
| ATAAGGTTGAGTGCAAGGCC+TGG | 0.527429 | 1.2:-69544198 | MS.gene60604:CDS |
| GAGAAAGACAAAGAAGAAGC+AGG | 0.540706 | 1.2:-69544431 | MS.gene60604:CDS |
| TTCATATAAGGTTGAGTGCA+AGG | 0.541798 | 1.2:-69544203 | MS.gene60604:CDS |
| ATTATTATTATAATCATCCC+AGG | 0.551179 | 1.2:+69544180 | None:intergenic |
| GAGTAGAACATGCAGAGCGA+CGG | 0.587047 | 1.2:+69544243 | None:intergenic |
| CATCTGCATTCTCTGCAATG+TGG | 0.593120 | 1.2:+69543957 | None:intergenic |
| ACAACAAAGACTTGATGAAG+AGG | 0.600172 | 1.2:-69544284 | MS.gene60604:CDS |
| ACGAACCACTTTACGAAGCA+GGG | 0.603396 | 1.2:-69544036 | MS.gene60604:CDS |
| ATTAAGTTCAATGGATAACA+TGG | 0.608143 | 1.2:-69544479 | None:intergenic |
| TCACTTCCCAAAACGGCAAG+TGG | 0.610829 | 1.2:-69544093 | MS.gene60604:CDS |
| CATTGCAGAGAATGCAGATG+AGG | 0.616435 | 1.2:-69543954 | MS.gene60604:CDS |
| TGAAGAGGGTGCTGCAATTG+CGG | 0.628142 | 1.2:-69544269 | MS.gene60604:CDS |
| ACCACTTTACGAAGCAGGGT+GGG | 0.630797 | 1.2:-69544032 | MS.gene60604:CDS |
| CTAATCTAGATGGTAGCACA+TGG | 0.631528 | 1.2:-69544120 | MS.gene60604:CDS |
| CAACAAAGACTTGATGAAGA+GGG | 0.641834 | 1.2:-69544283 | MS.gene60604:CDS |
| AACCACTTTACGAAGCAGGG+TGG | 0.676621 | 1.2:-69544033 | MS.gene60604:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATCTTGATTTCTTCAT+GGG | - | chr1.2:69544243-69544262 | MS.gene60604:CDS | 20.0% |
| !! | ATTATTATTATAATCATCCC+AGG | + | chr1.2:69544229-69544248 | None:intergenic | 20.0% |
| !! | TAATAATCTTGATTTCTTCA+TGG | - | chr1.2:69544242-69544261 | MS.gene60604:CDS | 20.0% |
| ! | ATAACATGGCATGTAATAAA+GGG | - | chr1.2:69543941-69543960 | MS.gene60604:CDS | 25.0% |
| AAGCTGAAAAGAAAAAGAGA+CGG | - | chr1.2:69544025-69544044 | MS.gene60604:CDS | 30.0% | |
| AGACTCAGATGATTCATATA+AGG | - | chr1.2:69544191-69544210 | MS.gene60604:CDS | 30.0% | |
| GATAACATGGCATGTAATAA+AGG | - | chr1.2:69543940-69543959 | MS.gene60604:CDS | 30.0% | |
| AATCTTGATTTCTTCATGGG+TGG | - | chr1.2:69544246-69544265 | MS.gene60604:CDS | 35.0% | |
| ACAACAAAGACTTGATGAAG+AGG | - | chr1.2:69544122-69544141 | MS.gene60604:CDS | 35.0% | |
| AGATCAACAGAGAATCTTGT+AGG | + | chr1.2:69544402-69544421 | None:intergenic | 35.0% | |
| ATCTTGATTTCTTCATGGGT+GGG | - | chr1.2:69544247-69544266 | MS.gene60604:CDS | 35.0% | |
| CAACAAAGACTTGATGAAGA+GGG | - | chr1.2:69544123-69544142 | MS.gene60604:CDS | 35.0% | |
| TGAAAAGAAAAAGAGACGGT+TGG | - | chr1.2:69544029-69544048 | MS.gene60604:CDS | 35.0% | |
| TTCATATAAGGTTGAGTGCA+AGG | - | chr1.2:69544203-69544222 | MS.gene60604:CDS | 35.0% | |
| ! | TTTTTCCAGTTCCGATATGA+TGG | + | chr1.2:69544079-69544098 | None:intergenic | 35.0% |
| CATGTGCTACCATCTAGATT+AGG | + | chr1.2:69544288-69544307 | None:intergenic | 40.0% | |
| CTAATCTAGATGGTAGCACA+TGG | - | chr1.2:69544286-69544305 | MS.gene60604:CDS | 40.0% | |
| GAGAAAGACAAAGAAGAAGC+AGG | - | chr1.2:69543975-69543994 | MS.gene60604:CDS | 40.0% | |
| GCTTGCTTTCCTAATCTAGA+TGG | - | chr1.2:69544276-69544295 | MS.gene60604:CDS | 40.0% | |
| TACTTCTGCTGCCATCATAT+CGG | - | chr1.2:69544065-69544084 | MS.gene60604:CDS | 40.0% | |
| TTATAGCAGCACAAGCAGTT+AGG | - | chr1.2:69544421-69544440 | MS.gene60604:CDS | 40.0% | |
| ACGAACCACTTTACGAAGCA+GGG | - | chr1.2:69544370-69544389 | MS.gene60604:CDS | 45.0% | |
| CAGAGAATGCAGATGAGGAT+AGG | - | chr1.2:69544457-69544476 | MS.gene60604:CDS | 45.0% | |
| CATCTGCATTCTCTGCAATG+TGG | + | chr1.2:69544452-69544471 | None:intergenic | 45.0% | |
| CATTGCAGAGAATGCAGATG+AGG | - | chr1.2:69544452-69544471 | MS.gene60604:CDS | 45.0% | |
| ! | CGCATTGTTTTCGAAAGGTC+CGG | + | chr1.2:69544349-69544368 | None:intergenic | 45.0% |
| ! | TCGTGCGCATTGTTTTCGAA+AGG | + | chr1.2:69544354-69544373 | None:intergenic | 45.0% |
| !! | AGATAGACCACTTGCCGTTT+TGG | + | chr1.2:69544323-69544342 | None:intergenic | 45.0% |
| !!! | GATAGACCACTTGCCGTTTT+GGG | + | chr1.2:69544322-69544341 | None:intergenic | 45.0% |
| AACCACTTTACGAAGCAGGG+TGG | - | chr1.2:69544373-69544392 | MS.gene60604:CDS | 50.0% | |
| ACCACTTTACGAAGCAGGGT+GGG | - | chr1.2:69544374-69544393 | MS.gene60604:CDS | 50.0% | |
| ATAAGGTTGAGTGCAAGGCC+TGG | - | chr1.2:69544208-69544227 | MS.gene60604:CDS | 50.0% | |
| CACGAACCACTTTACGAAGC+AGG | - | chr1.2:69544369-69544388 | MS.gene60604:CDS | 50.0% | |
| GAAGCAGGTGAAAGATGAGC+TGG | - | chr1.2:69543990-69544009 | MS.gene60604:CDS | 50.0% | |
| GAGACGGTTGGAAAAAGCTC+TGG | - | chr1.2:69544041-69544060 | MS.gene60604:CDS | 50.0% | |
| GAGTAGAACATGCAGAGCGA+CGG | + | chr1.2:69544166-69544185 | None:intergenic | 50.0% | |
| TAAGGTTGAGTGCAAGGCCT+GGG | - | chr1.2:69544209-69544228 | MS.gene60604:CDS | 50.0% | |
| TCACTTCCCAAAACGGCAAG+TGG | - | chr1.2:69544313-69544332 | MS.gene60604:CDS | 50.0% | |
| TGCTGCCATCATATCGGAAC+TGG | - | chr1.2:69544071-69544090 | MS.gene60604:CDS | 50.0% | |
| TGGTCCGTCACTTCCCAAAA+CGG | - | chr1.2:69544306-69544325 | MS.gene60604:CDS | 50.0% | |
| !! | TGAAGAGGGTGCTGCAATTG+CGG | - | chr1.2:69544137-69544156 | MS.gene60604:CDS | 50.0% |
| !!! | CTTGCCGTTTTGGGAAGTGA+CGG | + | chr1.2:69544313-69544332 | None:intergenic | 50.0% |
| GGCAAGTGGTCTATCTCGTC+CGG | - | chr1.2:69544327-69544346 | MS.gene60604:CDS | 55.0% | |
| TCCCACCCTGCTTCGTAAAG+TGG | + | chr1.2:69544378-69544397 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 69543937 | 69544491 | 69543937 | ID=MS.gene60604 |
| chr1.2 | mRNA | 69543937 | 69544491 | 69543937 | ID=MS.gene60604.t1;Parent=MS.gene60604 |
| chr1.2 | exon | 69543937 | 69544491 | 69543937 | ID=MS.gene60604.t1.exon1;Parent=MS.gene60604.t1 |
| chr1.2 | CDS | 69543937 | 69544491 | 69543937 | ID=cds.MS.gene60604.t1;Parent=MS.gene60604.t1 |
| Gene Sequence |
| Protein sequence |
