Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061135.t1 | AFK38248.1 | 96.8 | 187 | 6 | 0 | 31 | 217 | 4 | 190 | 1.00E-70 | 276.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061135.t1 | I3SDA6 | 96.8 | 187 | 6 | 0 | 31 | 217 | 4 | 190 | 7.3e-71 | 276.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057208 | MS.gene061135 | 0.805938 | 1.05E-49 | -1.69E-46 |
| MS.gene061135 | MS.gene06643 | 0.80891 | 2.46E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene066746 | 0.809768 | 1.61E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene06857 | 0.803857 | 2.85E-49 | -1.69E-46 |
| MS.gene061135 | MS.gene073139 | 0.809721 | 1.64E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene21562 | 0.80695 | 6.41E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene22695 | 0.80621 | 9.19E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene35317 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene35323 | 0.818423 | 1.98E-52 | -1.69E-46 |
| MS.gene061135 | MS.gene39882 | 0.806081 | 9.78E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene44510 | 0.811188 | 7.93E-51 | -1.69E-46 |
| MS.gene061135 | MS.gene48037 | 0.800255 | 1.57E-48 | -1.69E-46 |
| MS.gene061135 | MS.gene49320 | 0.808315 | 3.29E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene52122 | 0.80325 | 3.81E-49 | -1.69E-46 |
| MS.gene061135 | MS.gene71945 | 0.843564 | 1.30E-58 | -1.69E-46 |
| MS.gene061135 | MS.gene74604 | 0.806127 | 9.57E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene84186 | 0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene061135 | MS.gene87292 | 0.802984 | 4.33E-49 | -1.69E-46 |
| MS.gene061135 | MS.gene92352 | 0.813712 | 2.23E-51 | -1.69E-46 |
| MS.gene061135 | MS.gene92981 | 0.800367 | 1.49E-48 | -1.69E-46 |
| MS.gene061135 | MS.gene97030 | 0.819081 | 1.40E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60604 | MS.gene061135 | PPI |
| MS.gene49145 | MS.gene061135 | PPI |
| MS.gene006242 | MS.gene061135 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061135.t1 | MTR_1g100090 | 97.122 | 139 | 4 | 0 | 31 | 169 | 4 | 142 | 2.37e-96 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061135.t1 | AT4G25670 | 54.054 | 185 | 81 | 3 | 34 | 217 | 1 | 182 | 1.45e-49 | 160 |
| MS.gene061135.t1 | AT4G25670 | 54.054 | 185 | 81 | 3 | 34 | 217 | 1 | 182 | 1.45e-49 | 160 |
| MS.gene061135.t1 | AT4G25690 | 51.795 | 195 | 73 | 5 | 34 | 217 | 1 | 185 | 4.82e-49 | 159 |
| MS.gene061135.t1 | AT4G25690 | 51.795 | 195 | 73 | 5 | 34 | 217 | 1 | 185 | 4.82e-49 | 159 |
| MS.gene061135.t1 | AT5G52550 | 50.273 | 183 | 68 | 6 | 49 | 217 | 181 | 354 | 6.47e-43 | 148 |
| MS.gene061135.t1 | AT5G52550 | 73.333 | 60 | 16 | 0 | 49 | 108 | 78 | 137 | 1.81e-17 | 80.1 |
Find 46 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCTCCTGTCGTTTCTTTC+TGG | 0.038238 | 1.4:+77649458 | MS.gene061135:CDS |
| GTCTCCTGTCGTTTCTTTCT+GGG | 0.246147 | 1.4:+77649459 | MS.gene061135:CDS |
| GATAACATGGCATGTAATAA+AGG | 0.286451 | 1.4:+77649711 | MS.gene061135:CDS |
| AGATAGACCACTTGCCGTTT+TGG | 0.291490 | 1.4:-77650091 | None:intergenic |
| TAATAATCTTGATTTCTTCA+TGG | 0.361966 | 1.4:+77650013 | MS.gene061135:CDS |
| TATGTCGTTATTAAGTTCAA+TGG | 0.374544 | 1.4:+77649689 | MS.gene061135:intron |
| AGACTCAGATGATTCATATA+AGG | 0.397214 | 1.4:+77649962 | MS.gene061135:CDS |
| TGCTGCCATCATATCGGAAC+TGG | 0.404529 | 1.4:+77649842 | MS.gene061135:CDS |
| AATAATCTTGATTTCTTCAT+GGG | 0.406348 | 1.4:+77650014 | MS.gene061135:CDS |
| CCATGTGCCACCATCTAGAT+TGG | 0.409184 | 1.4:-77650057 | None:intergenic |
| AGGATAGGATTCTCTTCTAA+TGG | 0.433534 | 1.4:+77650243 | MS.gene061135:CDS |
| GCTTGCTTTCCCAATCTAGA+TGG | 0.435934 | 1.4:+77650047 | MS.gene061135:CDS |
| TACTTCTGCTGCCATCATAT+CGG | 0.438376 | 1.4:+77649836 | MS.gene061135:CDS |
| GGCAAGTGGTCTATCTCGTC+TGG | 0.460260 | 1.4:+77650098 | MS.gene061135:CDS |
| ATAACATGGCATGTAATAAA+GGG | 0.468465 | 1.4:+77649712 | MS.gene061135:CDS |
| CACGAACCGCTTTACGAAGC+AGG | 0.476420 | 1.4:+77650140 | MS.gene061135:CDS |
| AGATCAACAGAGAATCTTGT+AGG | 0.486420 | 1.4:-77650170 | None:intergenic |
| ATCTTGATTTCTTCATGGGT+GGG | 0.489941 | 1.4:+77650018 | MS.gene061135:CDS |
| GAAGCAGGTGAAAGATGAGC+TGG | 0.492593 | 1.4:+77649761 | MS.gene061135:CDS |
| GGAAGAATGGGGCCAAGGGA+TGG | 0.500345 | 1.4:+77649403 | None:intergenic |
| TGGTCCGTCACTTCCCAAAA+CGG | 0.505701 | 1.4:+77650077 | MS.gene061135:CDS |
| AATCTTGATTTCTTCATGGG+TGG | 0.519569 | 1.4:+77650017 | MS.gene061135:CDS |
| CAGAGAATGCAGATGAGGAT+AGG | 0.524026 | 1.4:+77650228 | MS.gene061135:CDS |
| AGTTAGGAAGAATGGGGCCA+AGG | 0.534435 | 1.4:+77649398 | None:intergenic |
| AACCGCTTTACGAAGCAGGA+TGG | 0.534751 | 1.4:+77650144 | MS.gene061135:CDS |
| CATGTGCCACCATCTAGATT+GGG | 0.537324 | 1.4:-77650056 | None:intergenic |
| ACCGCTTTACGAAGCAGGAT+GGG | 0.538753 | 1.4:+77650145 | MS.gene061135:CDS |
| GAGAAAGACAAAGAAGAAGC+AGG | 0.540706 | 1.4:+77649746 | MS.gene061135:CDS |
| ATTATTATTATAATCATCCC+AGG | 0.556888 | 1.4:-77649997 | None:intergenic |
| GTTAGGAAGAATGGGGCCAA+GGG | 0.557056 | 1.4:+77649399 | None:intergenic |
| ATTGCATAGTTCGTAAAATG+AGG | 0.568760 | 1.4:+77649482 | MS.gene061135:CDS |
| GCACTCTTAGATCCATCCCT+TGG | 0.569872 | 1.4:-77649415 | None:intergenic |
| CCAATCTAGATGGTGGCACA+TGG | 0.577656 | 1.4:+77650057 | MS.gene061135:CDS |
| TGCTTTCCCAATCTAGATGG+TGG | 0.585039 | 1.4:+77650050 | MS.gene061135:CDS |
| TCCCATCCTGCTTCGTAAAG+CGG | 0.587174 | 1.4:-77650146 | None:intergenic |
| CATCTGCATTCTCTGCAATG+TGG | 0.593120 | 1.4:-77650220 | None:intergenic |
| ATAAGGTTGAGTGCAAGACC+TGG | 0.599344 | 1.4:+77649979 | MS.gene061135:CDS |
| ACAACAAAGACTTGATGAAG+AGG | 0.600172 | 1.4:+77649893 | MS.gene061135:CDS |
| ATTAAGTTCAATGGATAACA+TGG | 0.608143 | 1.4:+77649698 | MS.gene061135:intron |
| TCACTTCCCAAAACGGCAAG+TGG | 0.610829 | 1.4:+77650084 | MS.gene061135:CDS |
| CAATCCCAGAAAGAAACGAC+AGG | 0.612234 | 1.4:-77649463 | None:intergenic |
| CATTGCAGAGAATGCAGATG+AGG | 0.616435 | 1.4:+77650223 | MS.gene061135:CDS |
| AAGTAGAACATGCAGAGCGA+CGG | 0.623440 | 1.4:-77649934 | None:intergenic |
| TGAAGAGGGTGCTGCAATTG+CGG | 0.628142 | 1.4:+77649908 | MS.gene061135:CDS |
| TAAGGTTGAGTGCAAGACCT+GGG | 0.633266 | 1.4:+77649980 | MS.gene061135:CDS |
| CAACAAAGACTTGATGAAGA+GGG | 0.641834 | 1.4:+77649894 | MS.gene061135:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTAGCATAATAGTTTA+GGG | + | chr1.4:77649585-77649604 | MS.gene061135:intron | 15.0% |
| !! | TATTATTAGCATAATAGTTT+AGG | + | chr1.4:77649584-77649603 | MS.gene061135:intron | 15.0% |
| !! | AATAATCTTGATTTCTTCAT+GGG | + | chr1.4:77650014-77650033 | MS.gene061135:CDS | 20.0% |
| !! | ATTATTATTATAATCATCCC+AGG | - | chr1.4:77650000-77650019 | None:intergenic | 20.0% |
| !! | TAATAATCTTGATTTCTTCA+TGG | + | chr1.4:77650013-77650032 | MS.gene061135:CDS | 20.0% |
| !! | TTATTAGCATAATAGTTTAG+GGG | + | chr1.4:77649586-77649605 | MS.gene061135:intron | 20.0% |
| ! | ATAACATGGCATGTAATAAA+GGG | + | chr1.4:77649712-77649731 | MS.gene061135:CDS | 25.0% |
| ! | ATTAAGTTCAATGGATAACA+TGG | + | chr1.4:77649698-77649717 | MS.gene061135:intron | 25.0% |
| ! | GAAAATGTATACAACTCTAT+AGG | + | chr1.4:77649621-77649640 | MS.gene061135:intron | 25.0% |
| ! | TATGTCGTTATTAAGTTCAA+TGG | + | chr1.4:77649689-77649708 | MS.gene061135:intron | 25.0% |
| AAGCTGAAAAGAAAAAGAGA+CGG | + | chr1.4:77649796-77649815 | MS.gene061135:CDS | 30.0% | |
| AGACTCAGATGATTCATATA+AGG | + | chr1.4:77649962-77649981 | MS.gene061135:CDS | 30.0% | |
| ATTGCATAGTTCGTAAAATG+AGG | + | chr1.4:77649482-77649501 | MS.gene061135:CDS | 30.0% | |
| GATAACATGGCATGTAATAA+AGG | + | chr1.4:77649711-77649730 | MS.gene061135:CDS | 30.0% | |
| AATCTTGATTTCTTCATGGG+TGG | + | chr1.4:77650017-77650036 | MS.gene061135:CDS | 35.0% | |
| ACAACAAAGACTTGATGAAG+AGG | + | chr1.4:77649893-77649912 | MS.gene061135:CDS | 35.0% | |
| AGATCAACAGAGAATCTTGT+AGG | - | chr1.4:77650173-77650192 | None:intergenic | 35.0% | |
| ATCTTGATTTCTTCATGGGT+GGG | + | chr1.4:77650018-77650037 | MS.gene061135:CDS | 35.0% | |
| CAACAAAGACTTGATGAAGA+GGG | + | chr1.4:77649894-77649913 | MS.gene061135:CDS | 35.0% | |
| TGAAAAGAAAAAGAGACGGT+TGG | + | chr1.4:77649800-77649819 | MS.gene061135:CDS | 35.0% | |
| ! | AGGAGTTTGCTTGAAGAATA+GGG | + | chr1.4:77649641-77649660 | MS.gene061135:intron | 35.0% |
| ! | TAGGAGTTTGCTTGAAGAAT+AGG | + | chr1.4:77649640-77649659 | MS.gene061135:intron | 35.0% |
| ! | TTTTTCCAGTTCCGATATGA+TGG | - | chr1.4:77649850-77649869 | None:intergenic | 35.0% |
| GAGAAAGACAAAGAAGAAGC+AGG | + | chr1.4:77649746-77649765 | MS.gene061135:CDS | 40.0% | |
| TACTTCTGCTGCCATCATAT+CGG | + | chr1.4:77649836-77649855 | MS.gene061135:CDS | 40.0% | |
| ! | TCGTGCACATTGTTTTCGAA+AGG | - | chr1.4:77650125-77650144 | None:intergenic | 40.0% |
| AAGTAGAACATGCAGAGCGA+CGG | - | chr1.4:77649937-77649956 | None:intergenic | 45.0% | |
| ATAAGGTTGAGTGCAAGACC+TGG | + | chr1.4:77649979-77649998 | MS.gene061135:CDS | 45.0% | |
| CAATCCCAGAAAGAAACGAC+AGG | - | chr1.4:77649466-77649485 | None:intergenic | 45.0% | |
| CAGAGAATGCAGATGAGGAT+AGG | + | chr1.4:77650228-77650247 | MS.gene061135:CDS | 45.0% | |
| CATCTGCATTCTCTGCAATG+TGG | - | chr1.4:77650223-77650242 | None:intergenic | 45.0% | |
| CATGTGCCACCATCTAGATT+GGG | - | chr1.4:77650059-77650078 | None:intergenic | 45.0% | |
| CATTGCAGAGAATGCAGATG+AGG | + | chr1.4:77650223-77650242 | MS.gene061135:CDS | 45.0% | |
| GCTTGCTTTCCCAATCTAGA+TGG | + | chr1.4:77650047-77650066 | MS.gene061135:CDS | 45.0% | |
| GTATTCCTACAGACCTGAGT+GGG | + | chr1.4:77649504-77649523 | MS.gene061135:intron | 45.0% | |
| TAAGGTTGAGTGCAAGACCT+GGG | + | chr1.4:77649980-77649999 | MS.gene061135:CDS | 45.0% | |
| TGCTTTCCCAATCTAGATGG+TGG | + | chr1.4:77650050-77650069 | MS.gene061135:CDS | 45.0% | |
| ! | GTCTCCTGTCGTTTCTTTCT+GGG | + | chr1.4:77649459-77649478 | MS.gene061135:CDS | 45.0% |
| ! | TGTCTCCTGTCGTTTCTTTC+TGG | + | chr1.4:77649458-77649477 | MS.gene061135:CDS | 45.0% |
| !! | AGATAGACCACTTGCCGTTT+TGG | - | chr1.4:77650094-77650113 | None:intergenic | 45.0% |
| !!! | GATAGACCACTTGCCGTTTT+GGG | - | chr1.4:77650093-77650112 | None:intergenic | 45.0% |
| AACCGCTTTACGAAGCAGGA+TGG | + | chr1.4:77650144-77650163 | MS.gene061135:CDS | 50.0% | |
| ACCGCTTTACGAAGCAGGAT+GGG | + | chr1.4:77650145-77650164 | MS.gene061135:CDS | 50.0% | |
| CCATGTGCCACCATCTAGAT+TGG | - | chr1.4:77650060-77650079 | None:intergenic | 50.0% | |
| GAAGCAGGTGAAAGATGAGC+TGG | + | chr1.4:77649761-77649780 | MS.gene061135:CDS | 50.0% | |
| GAGACGGTTGGAAAAAGCTC+TGG | + | chr1.4:77649812-77649831 | MS.gene061135:CDS | 50.0% | |
| GCACTCTTAGATCCATCCCT+TGG | - | chr1.4:77649418-77649437 | None:intergenic | 50.0% | |
| GGTATTCCTACAGACCTGAG+TGG | + | chr1.4:77649503-77649522 | MS.gene061135:intron | 50.0% | |
| TCACTTCCCAAAACGGCAAG+TGG | + | chr1.4:77650084-77650103 | MS.gene061135:CDS | 50.0% | |
| TCCCATCCTGCTTCGTAAAG+CGG | - | chr1.4:77650149-77650168 | None:intergenic | 50.0% | |
| TGCTGCCATCATATCGGAAC+TGG | + | chr1.4:77649842-77649861 | MS.gene061135:CDS | 50.0% | |
| TGGTCCGTCACTTCCCAAAA+CGG | + | chr1.4:77650077-77650096 | MS.gene061135:CDS | 50.0% | |
| ! | CCAATCTAGATGGTGGCACA+TGG | + | chr1.4:77650057-77650076 | MS.gene061135:CDS | 50.0% |
| !! | TGAAGAGGGTGCTGCAATTG+CGG | + | chr1.4:77649908-77649927 | MS.gene061135:CDS | 50.0% |
| !!! | CTTGCCGTTTTGGGAAGTGA+CGG | - | chr1.4:77650084-77650103 | None:intergenic | 50.0% |
| ACAGACCTGAGTGGGTGTAG+TGG | + | chr1.4:77649512-77649531 | MS.gene061135:intron | 55.0% | |
| ACCAACCACTACACCCACTC+AGG | - | chr1.4:77649520-77649539 | None:intergenic | 55.0% | |
| ACCTGAGTGGGTGTAGTGGT+TGG | + | chr1.4:77649516-77649535 | MS.gene061135:intron | 55.0% | |
| CACGAACCGCTTTACGAAGC+AGG | + | chr1.4:77650140-77650159 | MS.gene061135:CDS | 55.0% | |
| CTACACCCACTCAGGTCTGT+AGG | - | chr1.4:77649512-77649531 | None:intergenic | 55.0% | |
| GGCAAGTGGTCTATCTCGTC+TGG | + | chr1.4:77650098-77650117 | MS.gene061135:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 77649409 | 77650262 | 77649409 | ID=MS.gene061135 |
| chr1.4 | mRNA | 77649409 | 77650262 | 77649409 | ID=MS.gene061135.t1;Parent=MS.gene061135 |
| chr1.4 | exon | 77649409 | 77649503 | 77649409 | ID=MS.gene061135.t1.exon1;Parent=MS.gene061135.t1 |
| chr1.4 | CDS | 77649409 | 77649503 | 77649409 | ID=cds.MS.gene061135.t1;Parent=MS.gene061135.t1 |
| chr1.4 | exon | 77649704 | 77650262 | 77649704 | ID=MS.gene061135.t1.exon2;Parent=MS.gene061135.t1 |
| chr1.4 | CDS | 77649704 | 77650262 | 77649704 | ID=cds.MS.gene061135.t1;Parent=MS.gene061135.t1 |
| Gene Sequence |
| Protein sequence |
