Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49145.t1 | AFK38248.1 | 97.3 | 184 | 5 | 0 | 1 | 184 | 7 | 190 | 1.20E-69 | 272.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49145.t1 | I3SDA6 | 97.3 | 184 | 5 | 0 | 1 | 184 | 7 | 190 | 9.0e-70 | 272.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049419 | MS.gene49145 | -0.801681 | 8.03E-49 | -1.69E-46 |
| MS.gene049680 | MS.gene49145 | 0.800181 | 1.63E-48 | -1.69E-46 |
| MS.gene053963 | MS.gene49145 | 0.801178 | 1.02E-48 | -1.69E-46 |
| MS.gene058008 | MS.gene49145 | 0.808288 | 3.33E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene49145 | 0.803642 | 3.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006242 | MS.gene49145 | PPI |
| MS.gene60604 | MS.gene49145 | PPI |
| MS.gene49145 | MS.gene061135 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49145.t1 | MTR_1g100090 | 97.794 | 136 | 3 | 0 | 1 | 136 | 7 | 142 | 2.17e-95 | 274 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49145.t1 | AT4G25670 | 54.054 | 185 | 81 | 3 | 1 | 184 | 1 | 182 | 4.91e-50 | 160 |
| MS.gene49145.t1 | AT4G25670 | 54.054 | 185 | 81 | 3 | 1 | 184 | 1 | 182 | 4.91e-50 | 160 |
| MS.gene49145.t1 | AT4G25690 | 51.795 | 195 | 73 | 5 | 1 | 184 | 1 | 185 | 1.24e-49 | 159 |
| MS.gene49145.t1 | AT4G25690 | 51.795 | 195 | 73 | 5 | 1 | 184 | 1 | 185 | 1.24e-49 | 159 |
| MS.gene49145.t1 | AT5G52550 | 50.273 | 183 | 68 | 6 | 16 | 184 | 181 | 354 | 7.23e-44 | 149 |
| MS.gene49145.t1 | AT5G52550 | 73.333 | 60 | 16 | 0 | 16 | 75 | 78 | 137 | 9.32e-18 | 79.7 |
Find 38 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAACATGGCATGTAATAA+AGG | 0.286451 | 1.3:+68785609 | MS.gene49145:CDS |
| AGATAGACCACTTGCCGTTT+TGG | 0.291490 | 1.3:-68785989 | None:intergenic |
| TAATAATCTTGATTTCTTCA+TGG | 0.361966 | 1.3:+68785911 | MS.gene49145:CDS |
| TGCTGCCATCATATCGGAAC+TGG | 0.404529 | 1.3:+68785740 | MS.gene49145:CDS |
| AATAATCTTGATTTCTTCAT+GGG | 0.406348 | 1.3:+68785912 | MS.gene49145:CDS |
| GCTTGCTTTCCTAATCTAGA+TGG | 0.412460 | 1.3:+68785945 | MS.gene49145:CDS |
| AGGATAGGATTCTCTTCTAA+TGG | 0.433534 | 1.3:+68786141 | MS.gene49145:CDS |
| GGCAAGTGGTCTATCTCGTC+CGG | 0.448307 | 1.3:+68785996 | MS.gene49145:CDS |
| TACTTCTGCTGCCATCATAT+CGG | 0.450688 | 1.3:+68785734 | MS.gene49145:CDS |
| AGACTCGGATGATTCATATA+AGG | 0.459137 | 1.3:+68785860 | MS.gene49145:CDS |
| ATAACATGGCATGTAATAAA+GGG | 0.468465 | 1.3:+68785610 | MS.gene49145:CDS |
| CATGTGCCACCATCTAGATT+AGG | 0.473663 | 1.3:-68785954 | None:intergenic |
| CACGAACCGCTTTACGAAGC+AGG | 0.476420 | 1.3:+68786038 | MS.gene49145:CDS |
| GAAGCAGGTGAAAGATGAGT+TGG | 0.480043 | 1.3:+68785659 | MS.gene49145:CDS |
| AGATCAACAGAGAATCTTGT+AGG | 0.486420 | 1.3:-68786068 | None:intergenic |
| ATCTTGATTTCTTCATGGGT+GGG | 0.489941 | 1.3:+68785916 | MS.gene49145:CDS |
| TGGTCCGTCACTTCCCAAAA+CGG | 0.505701 | 1.3:+68785975 | MS.gene49145:CDS |
| AATCTTGATTTCTTCATGGG+TGG | 0.519569 | 1.3:+68785915 | MS.gene49145:CDS |
| CAGAGAATGCAGATGAGGAT+AGG | 0.524026 | 1.3:+68786126 | MS.gene49145:CDS |
| AACCGCTTTACGAAGCAGGA+TGG | 0.534751 | 1.3:+68786042 | MS.gene49145:CDS |
| ACCGCTTTACGAAGCAGGAT+GGG | 0.538753 | 1.3:+68786043 | MS.gene49145:CDS |
| GAGAAAGACAAAGAAGAAGC+AGG | 0.540706 | 1.3:+68785644 | MS.gene49145:CDS |
| ATTATTATTATAATCATCCC+AGG | 0.556888 | 1.3:-68785895 | None:intergenic |
| CTAATCTAGATGGTGGCACA+TGG | 0.572878 | 1.3:+68785955 | MS.gene49145:CDS |
| GCAAGTGGTCTATCTCGTCC+GGG | 0.576300 | 1.3:+68785997 | MS.gene49145:CDS |
| TCCCATCCTGCTTCGTAAAG+CGG | 0.587174 | 1.3:-68786044 | None:intergenic |
| CATCTGCATTCTCTGCAATG+TGG | 0.593120 | 1.3:-68786118 | None:intergenic |
| ATAAGGTTGAGTGCAAGACC+TGG | 0.599344 | 1.3:+68785877 | MS.gene49145:CDS |
| ACAACAAAGACTTGATGAAG+AGG | 0.600172 | 1.3:+68785791 | MS.gene49145:CDS |
| ATTAAGTTCAATGGATAACA+TGG | 0.608143 | 1.3:+68785596 | None:intergenic |
| TGCTTTCCTAATCTAGATGG+TGG | 0.608683 | 1.3:+68785948 | MS.gene49145:CDS |
| TCACTTCCCAAAACGGCAAG+TGG | 0.610829 | 1.3:+68785982 | MS.gene49145:CDS |
| CATTGCAGAGAATGCAGATG+AGG | 0.616435 | 1.3:+68786121 | MS.gene49145:CDS |
| AAGTAGAACATGCAGAGCGA+CGG | 0.623440 | 1.3:-68785832 | None:intergenic |
| TGAAGAGGGTGCTGCAATTG+CGG | 0.628142 | 1.3:+68785806 | MS.gene49145:CDS |
| TAAGGTTGAGTGCAAGACCT+GGG | 0.633266 | 1.3:+68785878 | MS.gene49145:CDS |
| CAACAAAGACTTGATGAAGA+GGG | 0.641834 | 1.3:+68785792 | MS.gene49145:CDS |
| TGTTCTACTTGATGAAGACT+CGG | 0.648329 | 1.3:+68785845 | MS.gene49145:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATCTTGATTTCTTCAT+GGG | + | chr1.3:68785912-68785931 | MS.gene49145:CDS | 20.0% |
| !! | ATTATTATTATAATCATCCC+AGG | - | chr1.3:68785898-68785917 | None:intergenic | 20.0% |
| !! | TAATAATCTTGATTTCTTCA+TGG | + | chr1.3:68785911-68785930 | MS.gene49145:CDS | 20.0% |
| ! | ATAACATGGCATGTAATAAA+GGG | + | chr1.3:68785610-68785629 | MS.gene49145:CDS | 25.0% |
| AAGCTGAAAAGAAAAAGAGA+CGG | + | chr1.3:68785694-68785713 | MS.gene49145:CDS | 30.0% | |
| GATAACATGGCATGTAATAA+AGG | + | chr1.3:68785609-68785628 | MS.gene49145:CDS | 30.0% | |
| AATCTTGATTTCTTCATGGG+TGG | + | chr1.3:68785915-68785934 | MS.gene49145:CDS | 35.0% | |
| ACAACAAAGACTTGATGAAG+AGG | + | chr1.3:68785791-68785810 | MS.gene49145:CDS | 35.0% | |
| AGACTCGGATGATTCATATA+AGG | + | chr1.3:68785860-68785879 | MS.gene49145:CDS | 35.0% | |
| AGATCAACAGAGAATCTTGT+AGG | - | chr1.3:68786071-68786090 | None:intergenic | 35.0% | |
| ATCTTGATTTCTTCATGGGT+GGG | + | chr1.3:68785916-68785935 | MS.gene49145:CDS | 35.0% | |
| CAACAAAGACTTGATGAAGA+GGG | + | chr1.3:68785792-68785811 | MS.gene49145:CDS | 35.0% | |
| TGAAAAGAAAAAGAGACGGT+TGG | + | chr1.3:68785698-68785717 | MS.gene49145:CDS | 35.0% | |
| TGTTCTACTTGATGAAGACT+CGG | + | chr1.3:68785845-68785864 | MS.gene49145:CDS | 35.0% | |
| ! | TCGTGCACATTGTTTTCAAA+AGG | - | chr1.3:68786023-68786042 | None:intergenic | 35.0% |
| ! | TTTTTCCAGTTCCGATATGA+TGG | - | chr1.3:68785748-68785767 | None:intergenic | 35.0% |
| GAGAAAGACAAAGAAGAAGC+AGG | + | chr1.3:68785644-68785663 | MS.gene49145:CDS | 40.0% | |
| GCTTGCTTTCCTAATCTAGA+TGG | + | chr1.3:68785945-68785964 | MS.gene49145:CDS | 40.0% | |
| TACTTCTGCTGCCATCATAT+CGG | + | chr1.3:68785734-68785753 | MS.gene49145:CDS | 40.0% | |
| TGCTTTCCTAATCTAGATGG+TGG | + | chr1.3:68785948-68785967 | MS.gene49145:CDS | 40.0% | |
| ! | CACATTGTTTTCAAAAGGCC+CGG | - | chr1.3:68786018-68786037 | None:intergenic | 40.0% |
| AAGTAGAACATGCAGAGCGA+CGG | - | chr1.3:68785835-68785854 | None:intergenic | 45.0% | |
| ATAAGGTTGAGTGCAAGACC+TGG | + | chr1.3:68785877-68785896 | MS.gene49145:CDS | 45.0% | |
| CAGAGAATGCAGATGAGGAT+AGG | + | chr1.3:68786126-68786145 | MS.gene49145:CDS | 45.0% | |
| CATCTGCATTCTCTGCAATG+TGG | - | chr1.3:68786121-68786140 | None:intergenic | 45.0% | |
| CATGTGCCACCATCTAGATT+AGG | - | chr1.3:68785957-68785976 | None:intergenic | 45.0% | |
| CATTGCAGAGAATGCAGATG+AGG | + | chr1.3:68786121-68786140 | MS.gene49145:CDS | 45.0% | |
| GAAGCAGGTGAAAGATGAGT+TGG | + | chr1.3:68785659-68785678 | MS.gene49145:CDS | 45.0% | |
| TAAGGTTGAGTGCAAGACCT+GGG | + | chr1.3:68785878-68785897 | MS.gene49145:CDS | 45.0% | |
| ! | CTAATCTAGATGGTGGCACA+TGG | + | chr1.3:68785955-68785974 | MS.gene49145:CDS | 45.0% |
| !! | AGATAGACCACTTGCCGTTT+TGG | - | chr1.3:68785992-68786011 | None:intergenic | 45.0% |
| !!! | GATAGACCACTTGCCGTTTT+GGG | - | chr1.3:68785991-68786010 | None:intergenic | 45.0% |
| AACCGCTTTACGAAGCAGGA+TGG | + | chr1.3:68786042-68786061 | MS.gene49145:CDS | 50.0% | |
| ACCGCTTTACGAAGCAGGAT+GGG | + | chr1.3:68786043-68786062 | MS.gene49145:CDS | 50.0% | |
| TCACTTCCCAAAACGGCAAG+TGG | + | chr1.3:68785982-68786001 | MS.gene49145:CDS | 50.0% | |
| TCCCATCCTGCTTCGTAAAG+CGG | - | chr1.3:68786047-68786066 | None:intergenic | 50.0% | |
| TGCTGCCATCATATCGGAAC+TGG | + | chr1.3:68785740-68785759 | MS.gene49145:CDS | 50.0% | |
| TGGTCCGTCACTTCCCAAAA+CGG | + | chr1.3:68785975-68785994 | MS.gene49145:CDS | 50.0% | |
| !! | TGAAGAGGGTGCTGCAATTG+CGG | + | chr1.3:68785806-68785825 | MS.gene49145:CDS | 50.0% |
| !!! | CTTGCCGTTTTGGGAAGTGA+CGG | - | chr1.3:68785982-68786001 | None:intergenic | 50.0% |
| CACGAACCGCTTTACGAAGC+AGG | + | chr1.3:68786038-68786057 | MS.gene49145:CDS | 55.0% | |
| GCAAGTGGTCTATCTCGTCC+GGG | + | chr1.3:68785997-68786016 | MS.gene49145:CDS | 55.0% | |
| GGCAAGTGGTCTATCTCGTC+CGG | + | chr1.3:68785996-68786015 | MS.gene49145:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 68785606 | 68786160 | 68785606 | ID=MS.gene49145 |
| chr1.3 | mRNA | 68785606 | 68786160 | 68785606 | ID=MS.gene49145.t1;Parent=MS.gene49145 |
| chr1.3 | exon | 68785606 | 68786160 | 68785606 | ID=MS.gene49145.t1.exon1;Parent=MS.gene49145.t1 |
| chr1.3 | CDS | 68785606 | 68786160 | 68785606 | ID=cds.MS.gene49145.t1;Parent=MS.gene49145.t1 |
| Gene Sequence |
| Protein sequence |
