AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007917

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007917.t1 MTR_8g463380 92.906 437 21 6 1 431 1 433 0.0 692
MS.gene007917.t1 MTR_2g090960 43.038 395 143 12 35 422 2 321 5.58e-79 248
MS.gene007917.t1 MTR_4g079580 37.726 387 157 12 40 414 5 319 1.02e-55 187
MS.gene007917.t1 MTR_2g078200 87.059 85 11 0 40 124 3 87 6.37e-48 166
MS.gene007917.t1 MTR_2g078200 48.148 135 57 5 306 431 164 294 2.48e-14 73.6
MS.gene007917.t1 MTR_7g015010 61.842 76 29 0 51 126 51 126 1.06e-25 107
MS.gene007917.t1 MTR_4g109660 63.235 68 25 0 51 118 50 117 2.90e-22 97.1
MS.gene007917.t1 MTR_3g026050 45.370 108 54 1 11 118 16 118 7.12e-22 97.1
MS.gene007917.t1 MTR_6g015350 64.706 68 24 0 51 118 43 110 2.23e-21 94.7
MS.gene007917.t1 MTR_4g111935 42.424 99 54 2 33 130 110 206 7.05e-17 82.4
MS.gene007917.t1 MTR_1g103380 55.738 61 27 0 56 116 200 260 2.10e-16 81.6
MS.gene007917.t1 MTR_6g017055 50.769 65 32 0 51 115 120 184 9.00e-16 79.3
MS.gene007917.t1 MTR_7g018500 56.140 57 25 0 59 115 122 178 8.86e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007917.t1 AT3G15030 47.846 441 137 19 38 431 26 420 1.78e-95 293
MS.gene007917.t1 AT3G15030 47.846 441 137 19 38 431 26 420 1.78e-95 293
MS.gene007917.t1 AT3G15030 47.846 441 137 19 38 431 26 420 1.78e-95 293
MS.gene007917.t1 AT3G15030 47.846 441 137 19 38 431 26 420 1.78e-95 293
MS.gene007917.t1 AT1G53230 47.368 418 142 18 37 431 29 391 7.10e-95 291
MS.gene007917.t1 AT1G53230 47.368 418 142 18 37 431 29 391 7.10e-95 291
MS.gene007917.t1 AT2G31070 45.374 227 67 7 37 253 9 188 1.76e-45 161
MS.gene007917.t1 AT4G18390 70.588 68 20 0 51 118 36 103 3.68e-26 108
MS.gene007917.t1 AT4G18390 70.588 68 20 0 51 118 36 103 3.68e-26 108
MS.gene007917.t1 AT3G02150 68.116 69 22 0 51 119 68 136 1.69e-24 102
MS.gene007917.t1 AT3G02150 68.116 69 22 0 51 119 68 136 3.42e-24 103
MS.gene007917.t1 AT1G30210 63.768 69 25 0 50 118 43 111 1.64e-22 98.2
MS.gene007917.t1 AT1G30210 63.768 69 25 0 50 118 43 111 1.64e-22 98.2
MS.gene007917.t1 AT5G60970 60.294 68 27 0 51 118 57 124 8.42e-22 96.7
MS.gene007917.t1 AT5G08070 58.824 68 28 0 51 118 27 94 3.22e-21 92.8
MS.gene007917.t1 AT5G08070 58.824 68 28 0 51 118 27 94 3.22e-21 92.8
MS.gene007917.t1 AT1G68800 51.471 68 33 0 53 120 108 175 2.22e-19 89.4
MS.gene007917.t1 AT1G68800 51.471 68 33 0 53 120 108 175 2.23e-19 89.7
MS.gene007917.t1 AT1G67260 52.113 71 34 0 52 122 82 152 9.87e-19 87.4
MS.gene007917.t1 AT1G67260 52.113 71 34 0 52 122 82 152 9.87e-19 87.4
MS.gene007917.t1 AT1G67260 52.113 71 34 0 52 122 82 152 1.39e-18 87.0
MS.gene007917.t1 AT3G18550 50.000 64 32 0 53 116 144 207 1.17e-13 72.8
MS.gene007917.t1 AT3G18550 50.000 64 32 0 53 116 144 207 1.17e-13 72.8
MS.gene007917.t1 AT3G18550 50.000 64 32 0 53 116 144 207 1.24e-13 72.8
MS.gene007917.t1 AT3G18550 50.000 64 32 0 53 116 144 207 1.29e-13 72.8

Find 130 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTATACCAGCACGAATTC+AGG 0.286283 8.3:-46640251 MS.gene007917:CDS
GTCTCTGGTGGTGGATTCTC+AGG 0.305280 8.3:-46640277 MS.gene007917:CDS
ATCCATCAGGCTTCTGTCTC+TGG 0.306407 8.3:-46640292 MS.gene007917:CDS
GTAACCAAGGGAGGAGTTTC+CGG 0.306811 8.3:+46640636 None:intergenic
GCACCGGCGTTTGGCGGTTA+TGG 0.331523 8.3:-46640439 MS.gene007917:CDS
GACGTCGTGGGCGTTTCAAC+TGG 0.345584 8.3:+46640820 None:intergenic
TCCGCTGCCGCTGATTCTCT+TGG 0.351084 8.3:+46641371 None:intergenic
TGGTGGCTCTGAAGCATAAT+AGG 0.365493 8.3:+46640697 None:intergenic
AGCGGCAGCGGAGGAGGAGG+AGG 0.369715 8.3:-46641360 MS.gene007917:CDS
ATCAGCGGCAGCGGAGGAGG+AGG 0.374612 8.3:-46641363 MS.gene007917:CDS
AGCCAATCGACTGCTTTACT+TGG 0.379997 8.3:+46641171 None:intergenic
GAGTTCGTCGATAGCAGATT+TGG 0.383791 8.3:+46641137 None:intergenic
GGAAGCAGAGGACGGTTTAT+CGG 0.389422 8.3:+46640204 None:intergenic
AGTACACCGGCACCGGCGTT+TGG 0.392086 8.3:-46640448 MS.gene007917:CDS
TGACTGGTAGAAGCGGTTAC+TGG 0.399280 8.3:+46641003 None:intergenic
GGTCACATTGTCCGTTCAAC+AGG 0.417152 8.3:-46641315 MS.gene007917:CDS
TGTTGTCCACCGGAGATTGC+CGG 0.418132 8.3:+46641028 None:intergenic
CTGTTGCTGTTCCTGTGTGC+TGG 0.422018 8.3:+46641439 None:intergenic
CAGGCTTCTGTCTCTGGTGG+TGG 0.423247 8.3:-46640286 MS.gene007917:CDS
AGAATCAGCGGCAGCGGAGG+AGG 0.424143 8.3:-46641366 MS.gene007917:CDS
ATATTCGCTGTCGCCATTGA+AGG 0.426291 8.3:+46640349 None:intergenic
CTTGGTTGTTGTTGTGGTGG+TGG 0.431039 8.3:+46640668 None:intergenic
AGAGTCGTACGCGGCGGTCA+CGG 0.437341 8.3:+46641250 None:intergenic
AACTCGCTCACCTCCCTGCT+TGG 0.437620 8.3:-46641119 MS.gene007917:CDS
GGTAACAGTGGCCATGGTGC+TGG 0.446213 8.3:-46640481 MS.gene007917:CDS
ACGAACTGAAGGATTGTAAC+TGG 0.446835 8.3:+46640384 None:intergenic
GAGTCGTACGCGGCGGTCAC+GGG 0.446966 8.3:+46641251 None:intergenic
TTCGCCGGAAACTCCTCCCT+TGG 0.457148 8.3:-46640640 MS.gene007917:CDS
TGTTGTGGTGGTGGTTGTCA+TGG 0.458311 8.3:+46640677 None:intergenic
ACAGAAGCCTGATGGATCAT+TGG 0.470560 8.3:+46640298 None:intergenic
ACACCGGCACCGGCGTTTGG+CGG 0.478717 8.3:-46640445 MS.gene007917:CDS
GTAGCTGTGGGGTTCCAAGC+AGG 0.479135 8.3:+46641105 None:intergenic
ATTTCCTTCTCAACAACAGT+TGG 0.480997 8.3:-46640935 MS.gene007917:CDS
AGAGGCGGAGATCTTGCTGC+TGG 0.481925 8.3:+46640740 None:intergenic
TAATGCCGCTGAGCGGTGAC+TGG 0.483807 8.3:+46640987 None:intergenic
GGTTACGACGGTGGTGGAAG+TGG 0.485371 8.3:-46640619 MS.gene007917:CDS
ACAATCCTTCAGTTCGTGCT+TGG 0.487224 8.3:-46640378 MS.gene007917:CDS
CGTATACCAGCACGAATTCA+GGG 0.492711 8.3:-46640250 MS.gene007917:CDS
TCTTTCGCCAATGATCCATC+AGG 0.495748 8.3:-46640305 MS.gene007917:CDS
TCCAAGAGAATCAGCGGCAG+CGG 0.496376 8.3:-46641372 MS.gene007917:CDS
GCGGTCACGGGGACCTTTCG+CGG 0.503457 8.3:+46641263 None:intergenic
AAGAGAATCAGCGGCAGCGG+AGG 0.505536 8.3:-46641369 MS.gene007917:CDS
ATCTCCAACTGTTGTTGAGA+AGG 0.510561 8.3:+46640931 None:intergenic
TCCCTTGGTTACGACGGTGG+TGG 0.515901 8.3:-46640625 MS.gene007917:CDS
CTGCTGCTGCTCAGACCACA+AGG 0.518049 8.3:+46640591 None:intergenic
CGGTAGATTGCATAGAATGA+TGG 0.518097 8.3:-46640545 MS.gene007917:CDS
GGAGAAATCGTGGAGGTACA+AGG 0.518138 8.3:-46641339 MS.gene007917:CDS
AAAGCACTTGGTTGTTGTTG+TGG 0.519575 8.3:+46640662 None:intergenic
AATATTTCACCGGCAATCTC+CGG 0.520745 8.3:-46641037 MS.gene007917:CDS
TAACCGCCAAACGCCGGTGC+CGG 0.524727 8.3:+46640442 None:intergenic
GCTGTTCGAGAGAGTAAATC+CGG 0.527015 8.3:+46640775 None:intergenic
AGTTTCCGGCGAAAAGCACT+TGG 0.528802 8.3:+46640650 None:intergenic
TCTTCACCCTGAATTCGTGC+TGG 0.532864 8.3:+46640244 None:intergenic
GGTCTTTGCGTCCTGTTGAA+CGG 0.539979 8.3:+46641304 None:intergenic
TGCTTGGATAGATCCTTCAA+TGG 0.540229 8.3:-46640362 MS.gene007917:CDS
AGGAGGAGGAGGAGAAATCG+TGG 0.541859 8.3:-46641349 MS.gene007917:CDS
TGTGTTCAGTACACCGGCAC+CGG 0.543423 8.3:-46640455 MS.gene007917:CDS
GAAGGAAATTGTTGTTCTTG+TGG 0.545015 8.3:+46640949 None:intergenic
AGGATTGTAACTGGACTGAA+GGG 0.547857 8.3:+46640393 None:intergenic
CACCAGAGACAGAAGCCTGA+TGG 0.548735 8.3:+46640290 None:intergenic
GTTACTGGATTGTTGTCCAC+CGG 0.554367 8.3:+46641018 None:intergenic
GATTGCATAGAATGATGGCT+TGG 0.554776 8.3:-46640540 MS.gene007917:CDS
GCACTTGGTTGTTGTTGTGG+TGG 0.555413 8.3:+46640665 None:intergenic
GGCCAAGTAAAGCAGTCGAT+TGG 0.560622 8.3:-46641173 MS.gene007917:CDS
ACAACACACGACCAGCACAC+AGG 0.563376 8.3:-46641450 MS.gene007917:CDS
GCGAGAGTCGGAGGAAGCAG+AGG 0.568701 8.3:+46640192 None:intergenic
TAGCTGTGGGGTTCCAAGCA+GGG 0.568717 8.3:+46641106 None:intergenic
AGTAAAGTATGCACCGCGAA+AGG 0.570958 8.3:-46641276 MS.gene007917:CDS
GCCGCTGATTCTCTTGGACG+AGG 0.572452 8.3:+46641377 None:intergenic
AAGGATTGTAACTGGACTGA+AGG 0.573483 8.3:+46640392 None:intergenic
CATCAAGAGGAACATGATAA+CGG 0.575169 8.3:-46640565 MS.gene007917:CDS
AGTCGTACGCGGCGGTCACG+GGG 0.577670 8.3:+46641252 None:intergenic
GTAGAATTGAATCGCAGTGT+GGG 0.579117 8.3:+46641224 None:intergenic
GAGGCGGAGATCTTGCTGCT+GGG 0.582996 8.3:+46640741 None:intergenic
AAGATCGTCTCGGCTATGAC+AGG 0.584840 8.3:-46641194 MS.gene007917:CDS
TTCCACCACCGTCGTAACCA+AGG 0.587194 8.3:+46640623 None:intergenic
CAATCAATGGCGAGAGTCGG+AGG 0.587240 8.3:+46640183 None:intergenic
CGTAGAATTGAATCGCAGTG+TGG 0.587501 8.3:+46641223 None:intergenic
AAACAAATGAAATATTTCAC+CGG 0.588265 8.3:-46641047 MS.gene007917:CDS
CATCAGGCTTCTGTCTCTGG+TGG 0.590853 8.3:-46640289 MS.gene007917:CDS
AACTCCTCCCTTGGTTACGA+CGG 0.591967 8.3:-46640631 MS.gene007917:CDS
TCTATCCAAGCACGAACTGA+AGG 0.592530 8.3:+46640373 None:intergenic
AGTAGTAGCAGTGGGAAACA+TGG 0.592595 8.3:-46640898 MS.gene007917:CDS
TGTGGTGGTGGTTGTCATGG+TGG 0.600873 8.3:+46640680 None:intergenic
TTCCAATCAATGGCGAGAGT+CGG 0.602863 8.3:+46640180 None:intergenic
AGCACGAATTCAGGGTGAAG+AGG 0.603117 8.3:-46640242 MS.gene007917:CDS
TCCTCCCTTGGTTACGACGG+TGG 0.604523 8.3:-46640628 MS.gene007917:CDS
GCAGCAGCAGCATCATCAAG+AGG 0.605514 8.3:-46640578 MS.gene007917:CDS
GATGGTGGTAACAGTGGCCA+TGG 0.608651 8.3:-46640487 MS.gene007917:CDS
TGACCATAACCGCCAAACGC+CGG 0.611880 8.3:+46640436 None:intergenic
TCCACCACCGTCGTAACCAA+GGG 0.614166 8.3:+46640624 None:intergenic
GACTACAACAGTAGTAGCAG+TGG 0.615455 8.3:-46640907 MS.gene007917:CDS
TACGACGTGCAAGATCGTCT+CGG 0.617162 8.3:-46641204 MS.gene007917:CDS
CGGTGCATACTTTACTGTGA+CGG 0.619367 8.3:+46641283 None:intergenic
ATAACTGTGAAACGACGTCG+TGG 0.622223 8.3:+46640807 None:intergenic
TTGGCGAAAGATAGTGATGA+TGG 0.622368 8.3:+46640317 None:intergenic
CTTGAAAAGATTGCAATGAG+AGG 0.622677 8.3:+46640722 None:intergenic
GAGTCGGAGGAAGCAGAGGA+CGG 0.623160 8.3:+46640196 None:intergenic
ACCTCGTCCAAGAGAATCAG+CGG 0.626058 8.3:-46641378 MS.gene007917:CDS
TGTTGAAGAACATGTAGCTG+TGG 0.626291 8.3:+46641092 None:intergenic
GTTGAAGAACATGTAGCTGT+GGG 0.630226 8.3:+46641093 None:intergenic
ATTTCACCGGCAATCTCCGG+TGG 0.633397 8.3:-46641034 MS.gene007917:CDS
GGAAATTGTTGTTCTTGTGG+TGG 0.633432 8.3:+46640952 None:intergenic
TGGATTTGTGTTCAGTACAC+CGG 0.633611 8.3:-46640461 MS.gene007917:CDS
GAAAAGATTGCAATGAGAGG+CGG 0.637117 8.3:+46640725 None:intergenic
TCACAGTTATCAACAACCGC+CGG 0.637674 8.3:-46640794 MS.gene007917:CDS
GCGGAGATCTTGCTGCTGGG+TGG 0.647953 8.3:+46640744 None:intergenic
GTGGAAGTGGAAGCACCTTG+TGG 0.652297 8.3:-46640606 MS.gene007917:CDS
GTTAATGCTAATGCTGCAGA+TGG 0.654669 8.3:-46640505 MS.gene007917:CDS
ACCACCGTCGTAACCAAGGG+AGG 0.658945 8.3:+46640627 None:intergenic
ACTACAACAGTAGTAGCAGT+GGG 0.659751 8.3:-46640906 MS.gene007917:CDS
AATGCTAATGCTGCAGATGG+TGG 0.664064 8.3:-46640502 MS.gene007917:CDS
GGATTTACTCTCTCGAACAG+CGG 0.665722 8.3:-46640773 MS.gene007917:CDS
CTCGTTAGACACTGACATTG+CGG 0.667721 8.3:-46640863 MS.gene007917:CDS
TAACTGTGAAACGACGTCGT+GGG 0.673437 8.3:+46640808 None:intergenic
TGAGCGGTGACTGGTAGAAG+CGG 0.674706 8.3:+46640996 None:intergenic
TTCGCTCTAATGCCGCTGAG+CGG 0.675852 8.3:+46640980 None:intergenic
GCTGCAGATGGTGGTAACAG+TGG 0.701228 8.3:-46640493 MS.gene007917:CDS
GGATTGTAACTGGACTGAAG+GGG 0.704803 8.3:+46640394 None:intergenic
TGAACACAAATCCAGCACCA+TGG 0.705786 8.3:+46640470 None:intergenic
TTTACTCTCTCGAACAGCGG+CGG 0.705963 8.3:-46640770 MS.gene007917:CDS
TTCTACCAGTCACCGCTCAG+CGG 0.712108 8.3:-46640992 MS.gene007917:CDS
CTGTGGGGTTCCAAGCAGGG+AGG 0.716198 8.3:+46641109 None:intergenic
TGTGGGCTGAGAGTCGTACG+CGG 0.717449 8.3:+46641241 None:intergenic
AGGAGGAGGAGAAATCGTGG+AGG 0.725082 8.3:-46641346 MS.gene007917:CDS
GAAATCGTGGAGGTACAAGG+AGG 0.742186 8.3:-46641336 MS.gene007917:CDS
GGGCTGAGAGTCGTACGCGG+CGG 0.755550 8.3:+46641244 None:intergenic
GTTCGAGAGAGTAAATCCGG+CGG 0.767784 8.3:+46640778 None:intergenic
TTGAAGAACATGTAGCTGTG+GGG 0.767979 8.3:+46641094 None:intergenic
CAGGGTGAAGAGGAACACGA+CGG 0.776987 8.3:-46640232 MS.gene007917:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAATGAAATATTTCAC+CGG - chr8.3:46640598-46640617 MS.gene007917:CDS 20.0%
ATTTCCTTCTCAACAACAGT+TGG - chr8.3:46640710-46640729 MS.gene007917:CDS 35.0%
CATCAAGAGGAACATGATAA+CGG - chr8.3:46641080-46641099 MS.gene007917:CDS 35.0%
CTTGAAAAGATTGCAATGAG+AGG + chr8.3:46640926-46640945 None:intergenic 35.0%
! GAAGGAAATTGTTGTTCTTG+TGG + chr8.3:46640699-46640718 None:intergenic 35.0%
!!! ATGGTCAGTTTTTTACTCAG+AGG - chr8.3:46641225-46641244 MS.gene007917:CDS 35.0%
!!! TGGTCAGTTTTTTACTCAGA+GGG - chr8.3:46641226-46641245 MS.gene007917:CDS 35.0%
AAGGATTGTAACTGGACTGA+AGG + chr8.3:46641256-46641275 None:intergenic 40.0%
AATATTTCACCGGCAATCTC+CGG - chr8.3:46640608-46640627 MS.gene007917:CDS 40.0%
ACGAACTGAAGGATTGTAAC+TGG + chr8.3:46641264-46641283 None:intergenic 40.0%
ACTACAACAGTAGTAGCAGT+GGG - chr8.3:46640739-46640758 MS.gene007917:CDS 40.0%
AGGATTGTAACTGGACTGAA+GGG + chr8.3:46641255-46641274 None:intergenic 40.0%
ATCTCCAACTGTTGTTGAGA+AGG + chr8.3:46640717-46640736 None:intergenic 40.0%
CGGTAGATTGCATAGAATGA+TGG - chr8.3:46641100-46641119 MS.gene007917:CDS 40.0%
GAAAAGATTGCAATGAGAGG+CGG + chr8.3:46640923-46640942 None:intergenic 40.0%
GATTGCATAGAATGATGGCT+TGG - chr8.3:46641105-46641124 MS.gene007917:CDS 40.0%
GTAGAATTGAATCGCAGTGT+GGG + chr8.3:46640424-46640443 None:intergenic 40.0%
GTTAATGCTAATGCTGCAGA+TGG - chr8.3:46641140-46641159 MS.gene007917:CDS 40.0%
GTTGAAGAACATGTAGCTGT+GGG + chr8.3:46640555-46640574 None:intergenic 40.0%
TGCTTGGATAGATCCTTCAA+TGG - chr8.3:46641283-46641302 MS.gene007917:CDS 40.0%
TGGATTTGTGTTCAGTACAC+CGG - chr8.3:46641184-46641203 MS.gene007917:CDS 40.0%
TGTTGAAGAACATGTAGCTG+TGG + chr8.3:46640556-46640575 None:intergenic 40.0%
TTGAAGAACATGTAGCTGTG+GGG + chr8.3:46640554-46640573 None:intergenic 40.0%
TTGGCGAAAGATAGTGATGA+TGG + chr8.3:46641331-46641350 None:intergenic 40.0%
! AAAGCACTTGGTTGTTGTTG+TGG + chr8.3:46640986-46641005 None:intergenic 40.0%
! GGAAATTGTTGTTCTTGTGG+TGG + chr8.3:46640696-46640715 None:intergenic 40.0%
! GGGAAACATGGTTTTCTTCA+AGG - chr8.3:46640759-46640778 MS.gene007917:CDS 40.0%
!!! GGTCAGTTTTTTACTCAGAG+GGG - chr8.3:46641227-46641246 MS.gene007917:CDS 40.0%
AATGCTAATGCTGCAGATGG+TGG - chr8.3:46641143-46641162 MS.gene007917:CDS 45.0%
ACAATCCTTCAGTTCGTGCT+TGG - chr8.3:46641267-46641286 MS.gene007917:CDS 45.0%
AGCCAATCGACTGCTTTACT+TGG + chr8.3:46640477-46640496 None:intergenic 45.0%
AGTAAAGTATGCACCGCGAA+AGG - chr8.3:46640369-46640388 MS.gene007917:CDS 45.0%
AGTAGTAGCAGTGGGAAACA+TGG - chr8.3:46640747-46640766 MS.gene007917:CDS 45.0%
ATAACTGTGAAACGACGTCG+TGG + chr8.3:46640841-46640860 None:intergenic 45.0%
ATATTCGCTGTCGCCATTGA+AGG + chr8.3:46641299-46641318 None:intergenic 45.0%
CGGTGCATACTTTACTGTGA+CGG + chr8.3:46640365-46640384 None:intergenic 45.0%
CGTAGAATTGAATCGCAGTG+TGG + chr8.3:46640425-46640444 None:intergenic 45.0%
CGTATACCAGCACGAATTCA+GGG - chr8.3:46641395-46641414 MS.gene007917:CDS 45.0%
CTCGTTAGACACTGACATTG+CGG - chr8.3:46640782-46640801 MS.gene007917:CDS 45.0%
GACTACAACAGTAGTAGCAG+TGG - chr8.3:46640738-46640757 MS.gene007917:CDS 45.0%
GAGTTCGTCGATAGCAGATT+TGG + chr8.3:46640511-46640530 None:intergenic 45.0%
GCTGTTCGAGAGAGTAAATC+CGG + chr8.3:46640873-46640892 None:intergenic 45.0%
GGATTGTAACTGGACTGAAG+GGG + chr8.3:46641254-46641273 None:intergenic 45.0%
GGATTTACTCTCTCGAACAG+CGG - chr8.3:46640872-46640891 MS.gene007917:CDS 45.0%
GTTACTGGATTGTTGTCCAC+CGG + chr8.3:46640630-46640649 None:intergenic 45.0%
TAACTGTGAAACGACGTCGT+GGG + chr8.3:46640840-46640859 None:intergenic 45.0%
TCACAGTTATCAACAACCGC+CGG - chr8.3:46640851-46640870 MS.gene007917:CDS 45.0%
TCGTATACCAGCACGAATTC+AGG - chr8.3:46641394-46641413 MS.gene007917:CDS 45.0%
TCTATCCAAGCACGAACTGA+AGG + chr8.3:46641275-46641294 None:intergenic 45.0%
TCTTTCGCCAATGATCCATC+AGG - chr8.3:46641340-46641359 MS.gene007917:CDS 45.0%
TGAACACAAATCCAGCACCA+TGG + chr8.3:46641178-46641197 None:intergenic 45.0%
! AACAACCAAGTGCTTTTCGC+CGG - chr8.3:46640990-46641009 MS.gene007917:CDS 45.0%
! ACAGAAGCCTGATGGATCAT+TGG + chr8.3:46641350-46641369 None:intergenic 45.0%
!! TGGTGGCTCTGAAGCATAAT+AGG + chr8.3:46640951-46640970 None:intergenic 45.0%
AACTCCTCCCTTGGTTACGA+CGG - chr8.3:46641014-46641033 MS.gene007917:CDS 50.0%
AAGATCGTCTCGGCTATGAC+AGG - chr8.3:46640451-46640470 MS.gene007917:CDS 50.0%
ACCTCGTCCAAGAGAATCAG+CGG - chr8.3:46640267-46640286 MS.gene007917:CDS 50.0%
AGCACGAATTCAGGGTGAAG+AGG - chr8.3:46641403-46641422 MS.gene007917:CDS 50.0%
ATCCATCAGGCTTCTGTCTC+TGG - chr8.3:46641353-46641372 MS.gene007917:CDS 50.0%
GGAAGCAGAGGACGGTTTAT+CGG + chr8.3:46641444-46641463 None:intergenic 50.0%
GGCCAAGTAAAGCAGTCGAT+TGG - chr8.3:46640472-46640491 MS.gene007917:CDS 50.0%
GGTCACATTGTCCGTTCAAC+AGG - chr8.3:46640330-46640349 MS.gene007917:CDS 50.0%
GGTCTTTGCGTCCTGTTGAA+CGG + chr8.3:46640344-46640363 None:intergenic 50.0%
GTAACCAAGGGAGGAGTTTC+CGG + chr8.3:46641012-46641031 None:intergenic 50.0%
GTTCGAGAGAGTAAATCCGG+CGG + chr8.3:46640870-46640889 None:intergenic 50.0%
TACGACGTGCAAGATCGTCT+CGG - chr8.3:46640441-46640460 MS.gene007917:CDS 50.0%
TCTTCACCCTGAATTCGTGC+TGG + chr8.3:46641404-46641423 None:intergenic 50.0%
TGACTGGTAGAAGCGGTTAC+TGG + chr8.3:46640645-46640664 None:intergenic 50.0%
TTTACTCTCTCGAACAGCGG+CGG - chr8.3:46640875-46640894 MS.gene007917:CDS 50.0%
! CTTGGTTGTTGTTGTGGTGG+TGG + chr8.3:46640980-46640999 None:intergenic 50.0%
! GAAATCGTGGAGGTACAAGG+AGG - chr8.3:46640309-46640328 MS.gene007917:CDS 50.0%
! GCACTTGGTTGTTGTTGTGG+TGG + chr8.3:46640983-46641002 None:intergenic 50.0%
! GGAGAAATCGTGGAGGTACA+AGG - chr8.3:46640306-46640325 MS.gene007917:CDS 50.0%
!! AGTTTCCGGCGAAAAGCACT+TGG + chr8.3:46640998-46641017 None:intergenic 50.0%
!! TGTTGTGGTGGTGGTTGTCA+TGG + chr8.3:46640971-46640990 None:intergenic 50.0%
ACAACACACGACCAGCACAC+AGG - chr8.3:46640195-46640214 MS.gene007917:CDS 55.0%
AGGAGGAGGAGAAATCGTGG+AGG - chr8.3:46640299-46640318 MS.gene007917:CDS 55.0%
AGGAGGAGGAGGAGAAATCG+TGG - chr8.3:46640296-46640315 MS.gene007917:CDS 55.0%
ATTTCACCGGCAATCTCCGG+TGG - chr8.3:46640611-46640630 MS.gene007917:CDS 55.0%
CACCAGAGACAGAAGCCTGA+TGG + chr8.3:46641358-46641377 None:intergenic 55.0%
CAGGGTGAAGAGGAACACGA+CGG - chr8.3:46641413-46641432 MS.gene007917:CDS 55.0%
CATCAGGCTTCTGTCTCTGG+TGG - chr8.3:46641356-46641375 MS.gene007917:CDS 55.0%
GCAGCAGCAGCATCATCAAG+AGG - chr8.3:46641067-46641086 MS.gene007917:CDS 55.0%
TAGCTGTGGGGTTCCAAGCA+GGG + chr8.3:46640542-46640561 None:intergenic 55.0%
TCCAAGAGAATCAGCGGCAG+CGG - chr8.3:46640273-46640292 MS.gene007917:CDS 55.0%
TCCACCACCGTCGTAACCAA+GGG + chr8.3:46641024-46641043 None:intergenic 55.0%
TGACCATAACCGCCAAACGC+CGG + chr8.3:46641212-46641231 None:intergenic 55.0%
TGAGCGGTGACTGGTAGAAG+CGG + chr8.3:46640652-46640671 None:intergenic 55.0%
TGTGTTCAGTACACCGGCAC+CGG - chr8.3:46641190-46641209 MS.gene007917:CDS 55.0%
TGTTGTCCACCGGAGATTGC+CGG + chr8.3:46640620-46640639 None:intergenic 55.0%
TTCCACCACCGTCGTAACCA+AGG + chr8.3:46641025-46641044 None:intergenic 55.0%
TTCGCTCTAATGCCGCTGAG+CGG + chr8.3:46640668-46640687 None:intergenic 55.0%
TTCTACCAGTCACCGCTCAG+CGG - chr8.3:46640653-46640672 MS.gene007917:CDS 55.0%
! CTGTTGCTGTTCCTGTGTGC+TGG + chr8.3:46640209-46640228 None:intergenic 55.0%
! GATGGTGGTAACAGTGGCCA+TGG - chr8.3:46641158-46641177 MS.gene007917:CDS 55.0%
! GCTGCAGATGGTGGTAACAG+TGG - chr8.3:46641152-46641171 MS.gene007917:CDS 55.0%
!! GTCTCTGGTGGTGGATTCTC+AGG - chr8.3:46641368-46641387 MS.gene007917:CDS 55.0%
!! GTGGAAGTGGAAGCACCTTG+TGG - chr8.3:46641039-46641058 MS.gene007917:CDS 55.0%
!! TGTGGTGGTGGTTGTCATGG+TGG + chr8.3:46640968-46640987 None:intergenic 55.0%
AACTCGCTCACCTCCCTGCT+TGG - chr8.3:46640526-46640545 MS.gene007917:CDS 60.0%
AAGAGAATCAGCGGCAGCGG+AGG - chr8.3:46640276-46640295 MS.gene007917:CDS 60.0%
ACCACCGTCGTAACCAAGGG+AGG + chr8.3:46641021-46641040 None:intergenic 60.0%
AGAGGCGGAGATCTTGCTGC+TGG + chr8.3:46640908-46640927 None:intergenic 60.0%
CAGGCTTCTGTCTCTGGTGG+TGG - chr8.3:46641359-46641378 MS.gene007917:CDS 60.0%
CTGCTGCTGCTCAGACCACA+AGG + chr8.3:46641057-46641076 None:intergenic 60.0%
GACGTCGTGGGCGTTTCAAC+TGG + chr8.3:46640828-46640847 None:intergenic 60.0%
GAGGCGGAGATCTTGCTGCT+GGG + chr8.3:46640907-46640926 None:intergenic 60.0%
GAGTCGGAGGAAGCAGAGGA+CGG + chr8.3:46641452-46641471 None:intergenic 60.0%
GGTAACAGTGGCCATGGTGC+TGG - chr8.3:46641164-46641183 MS.gene007917:CDS 60.0%
GTAGCTGTGGGGTTCCAAGC+AGG + chr8.3:46640543-46640562 None:intergenic 60.0%
TAATGCCGCTGAGCGGTGAC+TGG + chr8.3:46640661-46640680 None:intergenic 60.0%
TCCCTTGGTTACGACGGTGG+TGG - chr8.3:46641020-46641039 MS.gene007917:CDS 60.0%
TCCTCCCTTGGTTACGACGG+TGG - chr8.3:46641017-46641036 MS.gene007917:CDS 60.0%
TTCGCCGGAAACTCCTCCCT+TGG - chr8.3:46641005-46641024 MS.gene007917:CDS 60.0%
! TGTGGGCTGAGAGTCGTACG+CGG + chr8.3:46640407-46640426 None:intergenic 60.0%
!! GCCGCTGATTCTCTTGGACG+AGG + chr8.3:46640271-46640290 None:intergenic 60.0%
!! GGTTACGACGGTGGTGGAAG+TGG - chr8.3:46641026-46641045 MS.gene007917:CDS 60.0%
!! TCCGCTGCCGCTGATTCTCT+TGG + chr8.3:46640277-46640296 None:intergenic 60.0%
AGAATCAGCGGCAGCGGAGG+AGG - chr8.3:46640279-46640298 MS.gene007917:CDS 65.0%
AGAGTCGTACGCGGCGGTCA+CGG + chr8.3:46640398-46640417 None:intergenic 65.0%
CTGTGGGGTTCCAAGCAGGG+AGG + chr8.3:46640539-46640558 None:intergenic 65.0%
GCGAGAGTCGGAGGAAGCAG+AGG + chr8.3:46641456-46641475 None:intergenic 65.0%
GCGGAGATCTTGCTGCTGGG+TGG + chr8.3:46640904-46640923 None:intergenic 65.0%
TAACCGCCAAACGCCGGTGC+CGG + chr8.3:46641206-46641225 None:intergenic 65.0%
TGCTGCTGCTGCTGCTGCTG+TGG + chr8.3:46640579-46640598 None:intergenic 65.0%
! GCACCGGCGTTTGGCGGTTA+TGG - chr8.3:46641206-46641225 MS.gene007917:CDS 65.0%
!! AGTACACCGGCACCGGCGTT+TGG - chr8.3:46641197-46641216 MS.gene007917:CDS 65.0%
AGTCGTACGCGGCGGTCACG+GGG + chr8.3:46640396-46640415 None:intergenic 70.0%
ATCAGCGGCAGCGGAGGAGG+AGG - chr8.3:46640282-46640301 MS.gene007917:CDS 70.0%
GAGTCGTACGCGGCGGTCAC+GGG + chr8.3:46640397-46640416 None:intergenic 70.0%
GCGGTCACGGGGACCTTTCG+CGG + chr8.3:46640385-46640404 None:intergenic 70.0%
GGGCTGAGAGTCGTACGCGG+CGG + chr8.3:46640404-46640423 None:intergenic 70.0%
!! ACACCGGCACCGGCGTTTGG+CGG - chr8.3:46641200-46641219 MS.gene007917:CDS 70.0%
AGCGGCAGCGGAGGAGGAGG+AGG - chr8.3:46640285-46640304 MS.gene007917:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 46640186 46641481 46640186 ID=MS.gene007917
chr8.3 mRNA 46640186 46641481 46640186 ID=MS.gene007917.t1;Parent=MS.gene007917
chr8.3 exon 46640186 46641481 46640186 ID=MS.gene007917.t1.exon1;Parent=MS.gene007917.t1
chr8.3 CDS 46640186 46641481 46640186 ID=cds.MS.gene007917.t1;Parent=MS.gene007917.t1
Gene Sequence

>MS.gene007917

ATGGGAGAAACAACACACGACCAGCACACAGGAACAGCAACAGCAACAGCTACATCAACATCAACAAAAACAGCAGCAACAACCTCGTCCAAGAGAATCAGCGGCAGCGGAGGAGGAGGAGGAGAAATCGTGGAGGTACAAGGAGGTCACATTGTCCGTTCAACAGGACGCAAAGACCGTCACAGTAAAGTATGCACCGCGAAAGGTCCCCGTGACCGCCGCGTACGACTCTCAGCCCACACTGCGATTCAATTCTACGACGTGCAAGATCGTCTCGGCTATGACAGGCCAAGTAAAGCAGTCGATTGGCTCATTAAAAAAGCCAAATCTGCTATCGACGAACTCGCTCACCTCCCTGCTTGGAACCCCACAGCTACATGTTCTTCAACACCACAGCAGCAGCAGCAGCAGCAAACAAATGAAATATTTCACCGGCAATCTCCGGTGGACAACAATCCAGTAACCGCTTCTACCAGTCACCGCTCAGCGGCATTAGAGCGAAGAATGCCACCACAAGAACAACAATTTCCTTCTCAACAACAGTTGGAGATAGACTACAACAGTAGTAGCAGTGGGAAACATGGTTTTCTTCAAGGCTCGTTAGACACTGACATTGCGGATACTATTAAGTCTTTTTTTCCAGTTGAAACGCCCACGACGTCGTTTCACAGTTATCAACAACCGCCGGATTTACTCTCTCGAACAGCGGCGGCAACCACCCAGCAGCAAGATCTCCGCCTCTCATTGCAATCTTTTCAAGATCCTATTATGCTTCAGAGCCACCATGACAACCACCACCACAACAACAACCAAGTGCTTTTCGCCGGAAACTCCTCCCTTGGTTACGACGGTGGTGGAAGTGGAAGCACCTTGTGGTCTGAGCAGCAGCAGCATCATCAAGAGGAACATGATAACGGTAGATTGCATAGAATGATGGCTTGGAATGCTAATAATGTTAATGCTAATGCTGCAGATGGTGGTAACAGTGGCCATGGTGCTGGATTTGTGTTCAGTACACCGGCACCGGCGTTTGGCGGTTATGGTCAGTTTTTTACTCAGAGGGGACCCCTTCAGTCCAGTTACAATCCTTCAGTTCGTGCTTGGATAGATCCTTCAATGGCGACAGCGAATATCGCTGATCACCATCATCACTATCTTTCGCCAATGATCCATCAGGCTTCTGTCTCTGGTGGTGGATTCTCAGGCTTTCGTATACCAGCACGAATTCAGGGTGAAGAGGAACACGACGGCGTATCCGATAAACCGTCCTCTGCTTCCTCCGACTCTCGCCATTGA
Protein sequence

>MS.gene007917.t1

MGETTHDQHTGTATATATSTSTKTAATTSSKRISGSGGGGGEIVEVQGGHIVRSTGRKDRHSKVCTAKGPRDRRVRLSAHTAIQFYDVQDRLGYDRPSKAVDWLIKKAKSAIDELAHLPAWNPTATCSSTPQQQQQQQTNEIFHRQSPVDNNPVTASTSHRSAALERRMPPQEQQFPSQQQLEIDYNSSSSGKHGFLQGSLDTDIADTIKSFFPVETPTTSFHSYQQPPDLLSRTAAATTQQQDLRLSLQSFQDPIMLQSHHDNHHHNNNQVLFAGNSSLGYDGGGSGSTLWSEQQQHHQEEHDNGRLHRMMAWNANNVNANAADGGNSGHGAGFVFSTPAPAFGGYGQFFTQRGPLQSSYNPSVRAWIDPSMATANIADHHHHYLSPMIHQASVSGGGFSGFRIPARIQGEEEHDGVSDKPSSASSDSRH