AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34255

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34255.t1 MTR_8g463380 94.240 434 20 3 1 430 1 433 0.0 699
MS.gene34255.t1 MTR_2g090960 42.677 396 144 12 33 421 2 321 5.02e-79 248
MS.gene34255.t1 MTR_4g079580 37.047 386 162 11 38 413 5 319 7.18e-56 188
MS.gene34255.t1 MTR_2g078200 87.059 85 11 0 38 122 3 87 5.60e-48 166
MS.gene34255.t1 MTR_2g078200 48.148 135 57 5 305 430 164 294 7.99e-14 72.0
MS.gene34255.t1 MTR_7g015010 61.842 76 29 0 49 124 51 126 1.09e-25 107
MS.gene34255.t1 MTR_4g109660 63.235 68 25 0 49 116 50 117 3.05e-22 97.1
MS.gene34255.t1 MTR_3g026050 45.714 105 52 1 12 116 19 118 6.72e-22 97.1
MS.gene34255.t1 MTR_6g015350 64.706 68 24 0 49 116 43 110 2.30e-21 94.7
MS.gene34255.t1 MTR_4g111935 42.424 99 54 2 31 128 110 206 7.40e-17 82.4
MS.gene34255.t1 MTR_1g103380 55.738 61 27 0 54 114 200 260 1.79e-16 81.6
MS.gene34255.t1 MTR_6g017055 50.769 65 32 0 49 113 120 184 8.54e-16 79.3
MS.gene34255.t1 MTR_7g018500 38.393 112 68 1 3 114 69 179 4.05e-15 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34255.t1 AT3G15030 48.073 441 137 19 36 430 26 420 1.50e-96 296
MS.gene34255.t1 AT3G15030 48.073 441 137 19 36 430 26 420 1.50e-96 296
MS.gene34255.t1 AT3G15030 48.073 441 137 19 36 430 26 420 1.50e-96 296
MS.gene34255.t1 AT3G15030 48.073 441 137 19 36 430 26 420 1.50e-96 296
MS.gene34255.t1 AT1G53230 47.482 417 144 16 35 430 29 391 5.79e-96 293
MS.gene34255.t1 AT1G53230 47.482 417 144 16 35 430 29 391 5.79e-96 293
MS.gene34255.t1 AT2G31070 37.113 291 72 10 35 262 9 251 3.83e-36 136
MS.gene34255.t1 AT4G18390 70.588 68 20 0 49 116 36 103 3.50e-26 109
MS.gene34255.t1 AT4G18390 70.588 68 20 0 49 116 36 103 3.50e-26 109
MS.gene34255.t1 AT3G02150 68.116 69 22 0 49 117 68 136 1.69e-24 102
MS.gene34255.t1 AT3G02150 68.116 69 22 0 49 117 68 136 3.39e-24 103
MS.gene34255.t1 AT1G30210 64.706 68 24 0 49 116 44 111 1.47e-22 98.2
MS.gene34255.t1 AT1G30210 64.706 68 24 0 49 116 44 111 1.47e-22 98.2
MS.gene34255.t1 AT5G60970 60.294 68 27 0 49 116 57 124 7.51e-22 96.7
MS.gene34255.t1 AT5G08070 58.824 68 28 0 49 116 27 94 3.32e-21 92.4
MS.gene34255.t1 AT5G08070 58.824 68 28 0 49 116 27 94 3.32e-21 92.4
MS.gene34255.t1 AT1G68800 51.471 68 33 0 51 118 108 175 2.11e-19 89.7
MS.gene34255.t1 AT1G68800 51.471 68 33 0 51 118 108 175 2.12e-19 89.4
MS.gene34255.t1 AT1G67260 52.113 71 34 0 50 120 82 152 1.06e-18 87.4
MS.gene34255.t1 AT1G67260 52.113 71 34 0 50 120 82 152 1.06e-18 87.4
MS.gene34255.t1 AT1G67260 52.113 71 34 0 50 120 82 152 1.36e-18 87.0
MS.gene34255.t1 AT3G18550 50.000 64 32 0 51 114 144 207 1.14e-13 72.8
MS.gene34255.t1 AT3G18550 50.000 64 32 0 51 114 144 207 1.14e-13 72.8
MS.gene34255.t1 AT3G18550 50.000 64 32 0 51 114 144 207 1.19e-13 72.8
MS.gene34255.t1 AT3G18550 50.000 64 32 0 51 114 144 207 1.19e-13 72.8

Find 130 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTATACCAGCACGAATTC+AGG 0.286283 8.4:-46996590 MS.gene34255:CDS
ATCCATCAGGCTTCTGTCTC+TGG 0.301766 8.4:-46996631 MS.gene34255:CDS
GTCTCTGGTGGTGGATTCTC+AGG 0.305280 8.4:-46996616 MS.gene34255:CDS
GTAACCAAGGGAGGAGTTTC+CGG 0.306811 8.4:+46996975 None:intergenic
GCGCCGGCGTTTGGCGGTTA+TGG 0.318409 8.4:-46996778 MS.gene34255:CDS
GACGTGGTGGGCGTTTCAAC+TGG 0.325465 8.4:+46997159 None:intergenic
TCCGCTGCCGCTGATTCTCT+TGG 0.351084 8.4:+46997713 None:intergenic
TGGTGACTCTGAAGCATAAT+AGG 0.363902 8.4:+46997039 None:intergenic
AGCGGCAGCGGAGGAGGAGG+AGG 0.369715 8.4:-46997702 MS.gene34255:CDS
CTGTTGCTGTTGCTGTGTGC+TGG 0.371934 8.4:+46997775 None:intergenic
ATCAGCGGCAGCGGAGGAGG+AGG 0.374612 8.4:-46997705 MS.gene34255:CDS
AGCCAATCGACTGCTTTACT+TGG 0.379997 8.4:+46997513 None:intergenic
AGTACACCGGCGCCGGCGTT+TGG 0.380968 8.4:-46996787 MS.gene34255:CDS
GAGTTCGTCGATAGCAGATT+TGG 0.383791 8.4:+46997479 None:intergenic
GGAAGCAGAGGACGGTTTAT+CGG 0.389422 8.4:+46996543 None:intergenic
TGACTGGTAGAAGCGGTTAC+TGG 0.399280 8.4:+46997345 None:intergenic
GGTCACATTGTCCGTTCAAC+AGG 0.408415 8.4:-46997657 MS.gene34255:CDS
CAGGCTTCTGTCTCTGGTGG+TGG 0.423247 8.4:-46996625 MS.gene34255:CDS
AGAATCAGCGGCAGCGGAGG+AGG 0.424143 8.4:-46997708 MS.gene34255:CDS
GGTTGTTGTTGTTGTGGTGG+TGG 0.431926 8.4:+46997010 None:intergenic
AGAGTCGTACGCGGCGGTCA+CGG 0.437341 8.4:+46997592 None:intergenic
AACTCGCTCACCTCCCTGCT+TGG 0.437620 8.4:-46997461 MS.gene34255:CDS
CTATCTCCAACTGTTGTTGT+TGG 0.445345 8.4:+46997268 None:intergenic
GGTAACAGTGGCCATGGTGC+TGG 0.446213 8.4:-46996820 MS.gene34255:CDS
ACGAACTGAAGGATTGTAAC+TGG 0.446835 8.4:+46996723 None:intergenic
GAGTCGTACGCGGCGGTCAC+GGG 0.446966 8.4:+46997593 None:intergenic
TATCTCCAACTGTTGTTGTT+GGG 0.450024 8.4:+46997269 None:intergenic
TTCGCCGGAAACTCCTCCCT+TGG 0.457148 8.4:-46996979 MS.gene34255:CDS
TGTTGTGGTGGTGGTTGTCA+TGG 0.458311 8.4:+46997019 None:intergenic
AGAGGCGGAGATCTTGCTGC+TGG 0.481925 8.4:+46997082 None:intergenic
TAATGCCGCTGAGCGGTGAC+TGG 0.483807 8.4:+46997329 None:intergenic
GGTTACGACGGTGGTGGAAG+TGG 0.485371 8.4:-46996958 MS.gene34255:CDS
ATATTCGCCGTCGCCATTGA+AGG 0.485547 8.4:+46996688 None:intergenic
ACAATCCTTCAGTTCGTGCT+TGG 0.487224 8.4:-46996717 MS.gene34255:CDS
ACACCGGCGCCGGCGTTTGG+CGG 0.489761 8.4:-46996784 MS.gene34255:CDS
CGTATACCAGCACGAATTCA+GGG 0.492711 8.4:-46996589 MS.gene34255:CDS
TCCTTCCCAACAACAACAGT+TGG 0.493806 8.4:-46997274 MS.gene34255:CDS
GGTCTTTCCTTCCTGTTGAA+CGG 0.495671 8.4:+46997646 None:intergenic
TCCAAGAGAATCAGCGGCAG+CGG 0.496376 8.4:-46997714 MS.gene34255:CDS
ACCGTTCGAGAGAGTAAATC+CGG 0.501307 8.4:+46997114 None:intergenic
GCGGTCACGGGGACCTTTCG+CGG 0.503457 8.4:+46997605 None:intergenic
AAGAGAATCAGCGGCAGCGG+AGG 0.505536 8.4:-46997711 MS.gene34255:CDS
TCTTTCGCCGATGATCCATC+AGG 0.505750 8.4:-46996644 MS.gene34255:CDS
TCCAACTGTTGTTGTTGGGA+AGG 0.512262 8.4:+46997273 None:intergenic
GCACTTGGTTGTTGTTGTTG+TGG 0.512670 8.4:+46997004 None:intergenic
AATGTTTCACCGGCAATCTC+CGG 0.513568 8.4:-46997379 MS.gene34255:CDS
CTTGGTTGTTGTTGTTGTGG+TGG 0.513896 8.4:+46997007 None:intergenic
GAAGGAAATTGTTGTTCTTG+TGG 0.514512 8.4:+46997291 None:intergenic
GTAGCTGTGGGATTCCAAGC+AGG 0.515567 8.4:+46997447 None:intergenic
TCCCTTGGTTACGACGGTGG+TGG 0.515901 8.4:-46996964 MS.gene34255:CDS
GGAGAAATCGTGGAGGTACA+AGG 0.518138 8.4:-46997681 MS.gene34255:CDS
AGTTTCCGGCGAAAAGCACT+TGG 0.528802 8.4:+46996989 None:intergenic
TCTTCACCCTGAATTCGTGC+TGG 0.532864 8.4:+46996583 None:intergenic
TAACCGCCAAACGCCGGCGC+CGG 0.534572 8.4:+46996781 None:intergenic
TGCTTGGATAGATCCTTCAA+TGG 0.540229 8.4:-46996701 MS.gene34255:CDS
AGGAGGAGGAGGAGAAATCG+TGG 0.541859 8.4:-46997691 MS.gene34255:CDS
CGGCAGATTGCATAGAATGA+TGG 0.543570 8.4:-46996884 MS.gene34255:CDS
TGTGTTCAGTACACCGGCGC+CGG 0.543712 8.4:-46996794 MS.gene34255:CDS
AGGATTGTAACTGGACTGAA+GGG 0.547857 8.4:+46996732 None:intergenic
CACCAGAGACAGAAGCCTGA+TGG 0.548735 8.4:+46996629 None:intergenic
GGCCAAGTAAAGCAGTCGAT+TGG 0.560622 8.4:-46997515 MS.gene34255:CDS
GATTGCATAGAATGATGGCT+TGG 0.567846 8.4:-46996879 MS.gene34255:CDS
GCGAGAGTCGGAGGAAGCAG+AGG 0.568701 8.4:+46996531 None:intergenic
ACAGAAGCCTGATGGATCAT+CGG 0.568719 8.4:+46996637 None:intergenic
AGTAAAGTATGCACCGCGAA+AGG 0.570958 8.4:-46997618 MS.gene34255:CDS
AAACAAATGAAATGTTTCAC+CGG 0.571359 8.4:-46997389 MS.gene34255:CDS
GCCGCTGATTCTCTTGGACG+AGG 0.572452 8.4:+46997719 None:intergenic
CATCAAGAGGAACATGATAA+CGG 0.573201 8.4:-46996904 MS.gene34255:CDS
AAGGATTGTAACTGGACTGA+AGG 0.573483 8.4:+46996731 None:intergenic
TCGGCGAAAGATAGTGATGA+TGG 0.573536 8.4:+46996656 None:intergenic
TGACCATAACCGCCAAACGC+CGG 0.576444 8.4:+46996775 None:intergenic
AGTCGTACGCGGCGGTCACG+GGG 0.577670 8.4:+46997594 None:intergenic
GTAGAATTGAATCGCAGTGT+GGG 0.579117 8.4:+46997566 None:intergenic
GAGGCGGAGATCTTGCTGCT+GGG 0.582996 8.4:+46997083 None:intergenic
AAGATCGTCTCGGCTATGAC+AGG 0.584840 8.4:-46997536 MS.gene34255:CDS
TTCCACCACCGTCGTAACCA+AGG 0.587194 8.4:+46996962 None:intergenic
CAATCAATGGCGAGAGTCGG+AGG 0.587240 8.4:+46996522 None:intergenic
CGTAGAATTGAATCGCAGTG+TGG 0.587501 8.4:+46997565 None:intergenic
TAGCTGTGGGATTCCAAGCA+GGG 0.590184 8.4:+46997448 None:intergenic
CATCAGGCTTCTGTCTCTGG+TGG 0.590853 8.4:-46996628 MS.gene34255:CDS
AACTCCTCCCTTGGTTACGA+CGG 0.591967 8.4:-46996970 MS.gene34255:CDS
TCTATCCAAGCACGAACTGA+AGG 0.592530 8.4:+46996712 None:intergenic
AGTAGTAGCAGTGGGAAACA+TGG 0.592595 8.4:-46997237 MS.gene34255:CDS
GGAAATTGTTGTTCTTGTGG+TGG 0.596044 8.4:+46997294 None:intergenic
CTCATTAGACACTGACATTG+CGG 0.602039 8.4:-46997202 MS.gene34255:CDS
TTCCAATCAATGGCGAGAGT+CGG 0.602863 8.4:+46996519 None:intergenic
AGCACGAATTCAGGGTGAAG+AGG 0.603117 8.4:-46996581 MS.gene34255:CDS
TGGATTTGTGTTCAGTACAC+CGG 0.603893 8.4:-46996800 MS.gene34255:CDS
TCCTCCCTTGGTTACGACGG+TGG 0.604523 8.4:-46996967 MS.gene34255:CDS
GCAGCAGCAGCATCATCAAG+AGG 0.605514 8.4:-46996917 MS.gene34255:CDS
GATGGTGGTAACAGTGGCCA+TGG 0.608651 8.4:-46996826 MS.gene34255:CDS
GATAGATCCTTCAATGGCGA+CGG 0.613580 8.4:-46996695 MS.gene34255:CDS
TCCACCACCGTCGTAACCAA+GGG 0.614166 8.4:+46996963 None:intergenic
GTTTCACCGGCAATCTCCGG+TGG 0.615022 8.4:-46997376 MS.gene34255:CDS
GACTACAACAGTAGTAGCAG+TGG 0.615455 8.4:-46997246 MS.gene34255:CDS
TACGACGTGCAAGATCGTCT+CGG 0.617162 8.4:-46997546 MS.gene34255:CDS
CGGTGCATACTTTACTGTGA+CGG 0.619367 8.4:+46997625 None:intergenic
CTTGAAAAGATTGCAATGAG+AGG 0.622677 8.4:+46997064 None:intergenic
GAGTCGGAGGAAGCAGAGGA+CGG 0.623160 8.4:+46996535 None:intergenic
ACCTCGTCCAAGAGAATCAG+CGG 0.626058 8.4:-46997720 MS.gene34255:CDS
TGTTGAAGAGCATGTAGCTG+TGG 0.628587 8.4:+46997434 None:intergenic
GCCGGATTTACTCTCTCGAA+CGG 0.634960 8.4:-46997115 MS.gene34255:CDS
GGATTTACTCTCTCGAACGG+TGG 0.636159 8.4:-46997112 MS.gene34255:CDS
GAAAAGATTGCAATGAGAGG+CGG 0.637117 8.4:+46997067 None:intergenic
TCACAGTTATCAACAACCGC+CGG 0.637674 8.4:-46997133 MS.gene34255:CDS
GCGGAGATCTTGCTGCTGGG+TGG 0.647953 8.4:+46997086 None:intergenic
ACATTGTCCGTTCAACAGGA+AGG 0.649388 8.4:-46997653 MS.gene34255:CDS
GTGGAAGTGGAAGCAACTTG+TGG 0.651942 8.4:-46996945 MS.gene34255:CDS
GTTAATGCTAATGCTGCAGA+TGG 0.654669 8.4:-46996844 MS.gene34255:CDS
ACCACCGTCGTAACCAAGGG+AGG 0.658945 8.4:+46996966 None:intergenic
ACTACAACAGTAGTAGCAGT+GGG 0.659751 8.4:-46997245 MS.gene34255:CDS
ATAACTGTGAAACGACGTGG+TGG 0.660445 8.4:+46997146 None:intergenic
AATGCTAATGCTGCAGATGG+TGG 0.664064 8.4:-46996841 MS.gene34255:CDS
TGAGCGGTGACTGGTAGAAG+CGG 0.674706 8.4:+46997338 None:intergenic
TTCGCTCTAATGCCGCTGAG+CGG 0.675852 8.4:+46997322 None:intergenic
GTTGAAGAGCATGTAGCTGT+GGG 0.676940 8.4:+46997435 None:intergenic
GCTGCAGATGGTGGTAACAG+TGG 0.701228 8.4:-46996832 MS.gene34255:CDS
GGATTGTAACTGGACTGAAG+GGG 0.704803 8.4:+46996733 None:intergenic
TGAACACAAATCCAGCACCA+TGG 0.705786 8.4:+46996809 None:intergenic
TTCTACCAGTCACCGCTCAG+CGG 0.712108 8.4:-46997334 MS.gene34255:CDS
TGTGGGCTGAGAGTCGTACG+CGG 0.717449 8.4:+46997583 None:intergenic
AAGATGGGAGAAACAACACA+CGG 0.717634 8.4:-46997798 MS.gene34255:CDS
CTGTGGGATTCCAAGCAGGG+AGG 0.723634 8.4:+46997451 None:intergenic
AGGAGGAGGAGAAATCGTGG+AGG 0.725082 8.4:-46997688 MS.gene34255:CDS
TAACTGTGAAACGACGTGGT+GGG 0.729729 8.4:+46997147 None:intergenic
GTTCGAGAGAGTAAATCCGG+CGG 0.741897 8.4:+46997117 None:intergenic
GAAATCGTGGAGGTACAAGG+AGG 0.742186 8.4:-46997678 MS.gene34255:CDS
GGGCTGAGAGTCGTACGCGG+CGG 0.755550 8.4:+46997586 None:intergenic
TTGATAACTGTGAAACGACG+TGG 0.755826 8.4:+46997143 None:intergenic
CAGGGTGAAGAGGAACACGA+CGG 0.776987 8.4:-46996571 MS.gene34255:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAACAAATGAAATGTTTCAC+CGG - chr8.4:46996931-46996950 MS.gene34255:CDS 25.0%
CATCAAGAGGAACATGATAA+CGG - chr8.4:46997416-46997435 MS.gene34255:CDS 35.0%
CTTGAAAAGATTGCAATGAG+AGG + chr8.4:46997259-46997278 None:intergenic 35.0%
! GAAGGAAATTGTTGTTCTTG+TGG + chr8.4:46997032-46997051 None:intergenic 35.0%
! TATCTCCAACTGTTGTTGTT+GGG + chr8.4:46997054-46997073 None:intergenic 35.0%
AAGGATTGTAACTGGACTGA+AGG + chr8.4:46997592-46997611 None:intergenic 40.0%
ACGAACTGAAGGATTGTAAC+TGG + chr8.4:46997600-46997619 None:intergenic 40.0%
ACTACAACAGTAGTAGCAGT+GGG - chr8.4:46997075-46997094 MS.gene34255:CDS 40.0%
AGGATTGTAACTGGACTGAA+GGG + chr8.4:46997591-46997610 None:intergenic 40.0%
CTATCTCCAACTGTTGTTGT+TGG + chr8.4:46997055-46997074 None:intergenic 40.0%
CTCATTAGACACTGACATTG+CGG - chr8.4:46997118-46997137 MS.gene34255:CDS 40.0%
GAAAAGATTGCAATGAGAGG+CGG + chr8.4:46997256-46997275 None:intergenic 40.0%
GATTGCATAGAATGATGGCT+TGG - chr8.4:46997441-46997460 MS.gene34255:CDS 40.0%
GTAGAATTGAATCGCAGTGT+GGG + chr8.4:46996757-46996776 None:intergenic 40.0%
GTTAATGCTAATGCTGCAGA+TGG - chr8.4:46997476-46997495 MS.gene34255:CDS 40.0%
TGCTTGGATAGATCCTTCAA+TGG - chr8.4:46997619-46997638 MS.gene34255:CDS 40.0%
TGGATTTGTGTTCAGTACAC+CGG - chr8.4:46997520-46997539 MS.gene34255:CDS 40.0%
! ATGGTCAGCTTTTTACTCAG+AGG - chr8.4:46997561-46997580 MS.gene34255:CDS 40.0%
! GGAAATTGTTGTTCTTGTGG+TGG + chr8.4:46997029-46997048 None:intergenic 40.0%
! GGGAAACATGGTTTTCTTCA+AGG - chr8.4:46997095-46997114 MS.gene34255:CDS 40.0%
! GTTACTGGATTGTTTTCCAC+CGG + chr8.4:46996963-46996982 None:intergenic 40.0%
! TGGTCAGCTTTTTACTCAGA+GGG - chr8.4:46997562-46997581 MS.gene34255:CDS 40.0%
! TTGATAACTGTGAAACGACG+TGG + chr8.4:46997180-46997199 None:intergenic 40.0%
!! TGGTGACTCTGAAGCATAAT+AGG + chr8.4:46997284-46997303 None:intergenic 40.0%
AATGCTAATGCTGCAGATGG+TGG - chr8.4:46997479-46997498 MS.gene34255:CDS 45.0%
AATGTTTCACCGGCAATCTC+CGG - chr8.4:46996941-46996960 MS.gene34255:CDS 45.0%
ACAATCCTTCAGTTCGTGCT+TGG - chr8.4:46997603-46997622 MS.gene34255:CDS 45.0%
ACATTGTCCGTTCAACAGGA+AGG - chr8.4:46996667-46996686 MS.gene34255:CDS 45.0%
ACCGTTCGAGAGAGTAAATC+CGG + chr8.4:46997209-46997228 None:intergenic 45.0%
AGCCAATCGACTGCTTTACT+TGG + chr8.4:46996810-46996829 None:intergenic 45.0%
AGTAAAGTATGCACCGCGAA+AGG - chr8.4:46996702-46996721 MS.gene34255:CDS 45.0%
AGTAGTAGCAGTGGGAAACA+TGG - chr8.4:46997083-46997102 MS.gene34255:CDS 45.0%
ATAACTGTGAAACGACGTGG+TGG + chr8.4:46997177-46997196 None:intergenic 45.0%
CGGCAGATTGCATAGAATGA+TGG - chr8.4:46997436-46997455 MS.gene34255:CDS 45.0%
CGGTGCATACTTTACTGTGA+CGG + chr8.4:46996698-46996717 None:intergenic 45.0%
CGTAGAATTGAATCGCAGTG+TGG + chr8.4:46996758-46996777 None:intergenic 45.0%
CGTATACCAGCACGAATTCA+GGG - chr8.4:46997731-46997750 MS.gene34255:CDS 45.0%
GACTACAACAGTAGTAGCAG+TGG - chr8.4:46997074-46997093 MS.gene34255:CDS 45.0%
GAGTTCGTCGATAGCAGATT+TGG + chr8.4:46996844-46996863 None:intergenic 45.0%
GATAGATCCTTCAATGGCGA+CGG - chr8.4:46997625-46997644 MS.gene34255:CDS 45.0%
GGATTGTAACTGGACTGAAG+GGG + chr8.4:46997590-46997609 None:intergenic 45.0%
GGTCTTTCCTTCCTGTTGAA+CGG + chr8.4:46996677-46996696 None:intergenic 45.0%
TAACTGTGAAACGACGTGGT+GGG + chr8.4:46997176-46997195 None:intergenic 45.0%
TCACAGTTATCAACAACCGC+CGG - chr8.4:46997187-46997206 MS.gene34255:CDS 45.0%
TCCTTCCCAACAACAACAGT+TGG - chr8.4:46997046-46997065 MS.gene34255:CDS 45.0%
TCGGCGAAAGATAGTGATGA+TGG + chr8.4:46997667-46997686 None:intergenic 45.0%
TCGTATACCAGCACGAATTC+AGG - chr8.4:46997730-46997749 MS.gene34255:CDS 45.0%
TCTATCCAAGCACGAACTGA+AGG + chr8.4:46997611-46997630 None:intergenic 45.0%
TGAACACAAATCCAGCACCA+TGG + chr8.4:46997514-46997533 None:intergenic 45.0%
! AACAACCAAGTGCTTTTCGC+CGG - chr8.4:46997326-46997345 MS.gene34255:CDS 45.0%
! ACAGAAGCCTGATGGATCAT+CGG + chr8.4:46997686-46997705 None:intergenic 45.0%
! CTTGGTTGTTGTTGTTGTGG+TGG + chr8.4:46997316-46997335 None:intergenic 45.0%
! GCACTTGGTTGTTGTTGTTG+TGG + chr8.4:46997319-46997338 None:intergenic 45.0%
! GGTCAGCTTTTTACTCAGAG+GGG - chr8.4:46997563-46997582 MS.gene34255:CDS 45.0%
! GTTGAAGAGCATGTAGCTGT+GGG + chr8.4:46996888-46996907 None:intergenic 45.0%
! TCCAACTGTTGTTGTTGGGA+AGG + chr8.4:46997050-46997069 None:intergenic 45.0%
! TGTTGAAGAGCATGTAGCTG+TGG + chr8.4:46996889-46996908 None:intergenic 45.0%
AACTCCTCCCTTGGTTACGA+CGG - chr8.4:46997350-46997369 MS.gene34255:CDS 50.0%
AAGATCGTCTCGGCTATGAC+AGG - chr8.4:46996784-46996803 MS.gene34255:CDS 50.0%
ACCTCGTCCAAGAGAATCAG+CGG - chr8.4:46996600-46996619 MS.gene34255:CDS 50.0%
AGCACGAATTCAGGGTGAAG+AGG - chr8.4:46997739-46997758 MS.gene34255:CDS 50.0%
ATATTCGCCGTCGCCATTGA+AGG + chr8.4:46997635-46997654 None:intergenic 50.0%
ATCCATCAGGCTTCTGTCTC+TGG - chr8.4:46997689-46997708 MS.gene34255:CDS 50.0%
GCCGGATTTACTCTCTCGAA+CGG - chr8.4:46997205-46997224 MS.gene34255:CDS 50.0%
GGAAGCAGAGGACGGTTTAT+CGG + chr8.4:46997780-46997799 None:intergenic 50.0%
GGATTTACTCTCTCGAACGG+TGG - chr8.4:46997208-46997227 MS.gene34255:CDS 50.0%
GGCCAAGTAAAGCAGTCGAT+TGG - chr8.4:46996805-46996824 MS.gene34255:CDS 50.0%
GGTCACATTGTCCGTTCAAC+AGG - chr8.4:46996663-46996682 MS.gene34255:CDS 50.0%
GTAACCAAGGGAGGAGTTTC+CGG + chr8.4:46997348-46997367 None:intergenic 50.0%
GTGGAAGTGGAAGCAACTTG+TGG - chr8.4:46997375-46997394 MS.gene34255:CDS 50.0%
GTTCGAGAGAGTAAATCCGG+CGG + chr8.4:46997206-46997225 None:intergenic 50.0%
TACGACGTGCAAGATCGTCT+CGG - chr8.4:46996774-46996793 MS.gene34255:CDS 50.0%
TAGCTGTGGGATTCCAAGCA+GGG + chr8.4:46996875-46996894 None:intergenic 50.0%
TCTTCACCCTGAATTCGTGC+TGG + chr8.4:46997740-46997759 None:intergenic 50.0%
TCTTTCGCCGATGATCCATC+AGG - chr8.4:46997676-46997695 MS.gene34255:CDS 50.0%
TGACTGGTAGAAGCGGTTAC+TGG + chr8.4:46996978-46996997 None:intergenic 50.0%
! GAAATCGTGGAGGTACAAGG+AGG - chr8.4:46996642-46996661 MS.gene34255:CDS 50.0%
! GGAGAAATCGTGGAGGTACA+AGG - chr8.4:46996639-46996658 MS.gene34255:CDS 50.0%
! GGTTGTTGTTGTTGTGGTGG+TGG + chr8.4:46997313-46997332 None:intergenic 50.0%
! TGTTTTCCACCGGAGATTGC+CGG + chr8.4:46996953-46996972 None:intergenic 50.0%
!! AGTTTCCGGCGAAAAGCACT+TGG + chr8.4:46997334-46997353 None:intergenic 50.0%
!! TGTTGTGGTGGTGGTTGTCA+TGG + chr8.4:46997304-46997323 None:intergenic 50.0%
AGGAGGAGGAGAAATCGTGG+AGG - chr8.4:46996632-46996651 MS.gene34255:CDS 55.0%
AGGAGGAGGAGGAGAAATCG+TGG - chr8.4:46996629-46996648 MS.gene34255:CDS 55.0%
CACCAGAGACAGAAGCCTGA+TGG + chr8.4:46997694-46997713 None:intergenic 55.0%
CAGGGTGAAGAGGAACACGA+CGG - chr8.4:46997749-46997768 MS.gene34255:CDS 55.0%
CATCAGGCTTCTGTCTCTGG+TGG - chr8.4:46997692-46997711 MS.gene34255:CDS 55.0%
GCAGCAGCAGCATCATCAAG+AGG - chr8.4:46997403-46997422 MS.gene34255:CDS 55.0%
GTAGCTGTGGGATTCCAAGC+AGG + chr8.4:46996876-46996895 None:intergenic 55.0%
TCCAAGAGAATCAGCGGCAG+CGG - chr8.4:46996606-46996625 MS.gene34255:CDS 55.0%
TCCACCACCGTCGTAACCAA+GGG + chr8.4:46997360-46997379 None:intergenic 55.0%
TGACCATAACCGCCAAACGC+CGG + chr8.4:46997548-46997567 None:intergenic 55.0%
TGAGCGGTGACTGGTAGAAG+CGG + chr8.4:46996985-46997004 None:intergenic 55.0%
TTCCACCACCGTCGTAACCA+AGG + chr8.4:46997361-46997380 None:intergenic 55.0%
TTCGCTCTAATGCCGCTGAG+CGG + chr8.4:46997001-46997020 None:intergenic 55.0%
TTCTACCAGTCACCGCTCAG+CGG - chr8.4:46996986-46997005 MS.gene34255:CDS 55.0%
! CTGTTGCTGTTGCTGTGTGC+TGG + chr8.4:46996548-46996567 None:intergenic 55.0%
! GATGGTGGTAACAGTGGCCA+TGG - chr8.4:46997494-46997513 MS.gene34255:CDS 55.0%
! GCTGCAGATGGTGGTAACAG+TGG - chr8.4:46997488-46997507 MS.gene34255:CDS 55.0%
!! GTCTCTGGTGGTGGATTCTC+AGG - chr8.4:46997704-46997723 MS.gene34255:CDS 55.0%
AACTCGCTCACCTCCCTGCT+TGG - chr8.4:46996859-46996878 MS.gene34255:CDS 60.0%
AAGAGAATCAGCGGCAGCGG+AGG - chr8.4:46996609-46996628 MS.gene34255:CDS 60.0%
ACCACCGTCGTAACCAAGGG+AGG + chr8.4:46997357-46997376 None:intergenic 60.0%
AGAGGCGGAGATCTTGCTGC+TGG + chr8.4:46997241-46997260 None:intergenic 60.0%
CAGGCTTCTGTCTCTGGTGG+TGG - chr8.4:46997695-46997714 MS.gene34255:CDS 60.0%
CTGTGGGATTCCAAGCAGGG+AGG + chr8.4:46996872-46996891 None:intergenic 60.0%
GAGGCGGAGATCTTGCTGCT+GGG + chr8.4:46997240-46997259 None:intergenic 60.0%
GAGTCGGAGGAAGCAGAGGA+CGG + chr8.4:46997788-46997807 None:intergenic 60.0%
GGTAACAGTGGCCATGGTGC+TGG - chr8.4:46997500-46997519 MS.gene34255:CDS 60.0%
GTTTCACCGGCAATCTCCGG+TGG - chr8.4:46996944-46996963 MS.gene34255:CDS 60.0%
TAATGCCGCTGAGCGGTGAC+TGG + chr8.4:46996994-46997013 None:intergenic 60.0%
TCCCTTGGTTACGACGGTGG+TGG - chr8.4:46997356-46997375 MS.gene34255:CDS 60.0%
TCCTCCCTTGGTTACGACGG+TGG - chr8.4:46997353-46997372 MS.gene34255:CDS 60.0%
TGTGTTCAGTACACCGGCGC+CGG - chr8.4:46997526-46997545 MS.gene34255:CDS 60.0%
TTCGCCGGAAACTCCTCCCT+TGG - chr8.4:46997341-46997360 MS.gene34255:CDS 60.0%
! TGTGGGCTGAGAGTCGTACG+CGG + chr8.4:46996740-46996759 None:intergenic 60.0%
!! GACGTGGTGGGCGTTTCAAC+TGG + chr8.4:46997164-46997183 None:intergenic 60.0%
!! GCCGCTGATTCTCTTGGACG+AGG + chr8.4:46996604-46996623 None:intergenic 60.0%
!! GGTTACGACGGTGGTGGAAG+TGG - chr8.4:46997362-46997381 MS.gene34255:CDS 60.0%
!! TCCGCTGCCGCTGATTCTCT+TGG + chr8.4:46996610-46996629 None:intergenic 60.0%
AGAATCAGCGGCAGCGGAGG+AGG - chr8.4:46996612-46996631 MS.gene34255:CDS 65.0%
AGAGTCGTACGCGGCGGTCA+CGG + chr8.4:46996731-46996750 None:intergenic 65.0%
GCGAGAGTCGGAGGAAGCAG+AGG + chr8.4:46997792-46997811 None:intergenic 65.0%
GCGGAGATCTTGCTGCTGGG+TGG + chr8.4:46997237-46997256 None:intergenic 65.0%
TGCTGCTGCTGCTGCTGCTG+TGG + chr8.4:46996912-46996931 None:intergenic 65.0%
AGTCGTACGCGGCGGTCACG+GGG + chr8.4:46996729-46996748 None:intergenic 70.0%
ATCAGCGGCAGCGGAGGAGG+AGG - chr8.4:46996615-46996634 MS.gene34255:CDS 70.0%
GAGTCGTACGCGGCGGTCAC+GGG + chr8.4:46996730-46996749 None:intergenic 70.0%
GCGGTCACGGGGACCTTTCG+CGG + chr8.4:46996718-46996737 None:intergenic 70.0%
GGGCTGAGAGTCGTACGCGG+CGG + chr8.4:46996737-46996756 None:intergenic 70.0%
TAACCGCCAAACGCCGGCGC+CGG + chr8.4:46997542-46997561 None:intergenic 70.0%
! GCGCCGGCGTTTGGCGGTTA+TGG - chr8.4:46997542-46997561 MS.gene34255:CDS 70.0%
!! AGTACACCGGCGCCGGCGTT+TGG - chr8.4:46997533-46997552 MS.gene34255:CDS 70.0%
AGCGGCAGCGGAGGAGGAGG+AGG - chr8.4:46996618-46996637 MS.gene34255:CDS 75.0%
!! ACACCGGCGCCGGCGTTTGG+CGG - chr8.4:46997536-46997555 MS.gene34255:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 46996525 46997817 46996525 ID=MS.gene34255
chr8.4 mRNA 46996525 46997817 46996525 ID=MS.gene34255.t1;Parent=MS.gene34255
chr8.4 exon 46996525 46997817 46996525 ID=MS.gene34255.t1.exon1;Parent=MS.gene34255.t1
chr8.4 CDS 46996525 46997817 46996525 ID=cds.MS.gene34255.t1;Parent=MS.gene34255.t1
Gene Sequence

>MS.gene34255

ATGGGAGAAACAACACACGGCCAGCACACAGCAACAGCAACAGCAACAGCTACATCAACAAAAACAGCAGCAACAACCTCGTCCAAGAGAATCAGCGGCAGCGGAGGAGGAGGAGGAGAAATCGTGGAGGTACAAGGAGGTCACATTGTCCGTTCAACAGGAAGGAAAGACCGTCACAGTAAAGTATGCACCGCGAAAGGTCCCCGTGACCGCCGCGTACGACTCTCAGCCCACACTGCGATTCAATTCTACGACGTGCAAGATCGTCTCGGCTATGACAGGCCAAGTAAAGCAGTCGATTGGCTCATTAAAAAAGCCAAATCTGCTATCGACGAACTCGCTCACCTCCCTGCTTGGAATCCCACAGCTACATGCTCTTCAACACCACAGCAGCAGCAGCAGCAGCAAACAAATGAAATGTTTCACCGGCAATCTCCGGTGGAAAACAATCCAGTAACCGCTTCTACCAGTCACCGCTCAGCGGCATTAGAGCGAAGAATGCCACCACAAGAACAACAATTTCCTTCCCAACAACAACAGTTGGAGATAGACTACAACAGTAGTAGCAGTGGGAAACATGGTTTTCTTCAAGGCTCATTAGACACTGACATTGCGGATACTATTAAGTCTTTTTTTCCAGTTGAAACGCCCACCACGTCGTTTCACAGTTATCAACAACCGCCGGATTTACTCTCTCGAACGGTGGCAACCACCCAGCAGCAAGATCTCCGCCTCTCATTGCAATCTTTTCAAGATCCTATTATGCTTCAGAGTCACCATGACAACCACCACCACAACAACAACAACCAAGTGCTTTTCGCCGGAAACTCCTCCCTTGGTTACGACGGTGGTGGAAGTGGAAGCAACTTGTGGTCTGAGCAGCAGCAGCATCATCAAGAGGAACATGATAACGGCAGATTGCATAGAATGATGGCTTGGAATGCTAATAATGTTAATGCTAATGCTGCAGATGGTGGTAACAGTGGCCATGGTGCTGGATTTGTGTTCAGTACACCGGCGCCGGCGTTTGGCGGTTATGGTCAGCTTTTTACTCAGAGGGGACCCCTTCAGTCCAGTTACAATCCTTCAGTTCGTGCTTGGATAGATCCTTCAATGGCGACGGCGAATATCGCTGATCACCATCATCACTATCTTTCGCCGATGATCCATCAGGCTTCTGTCTCTGGTGGTGGATTCTCAGGCTTTCGTATACCAGCACGAATTCAGGGTGAAGAGGAACACGACGGCGTATCCGATAAACCGTCCTCTGCTTCCTCCGACTCTCGCCATTGA
Protein sequence

>MS.gene34255.t1

MGETTHGQHTATATATATSTKTAATTSSKRISGSGGGGGEIVEVQGGHIVRSTGRKDRHSKVCTAKGPRDRRVRLSAHTAIQFYDVQDRLGYDRPSKAVDWLIKKAKSAIDELAHLPAWNPTATCSSTPQQQQQQQTNEMFHRQSPVENNPVTASTSHRSAALERRMPPQEQQFPSQQQQLEIDYNSSSSGKHGFLQGSLDTDIADTIKSFFPVETPTTSFHSYQQPPDLLSRTVATTQQQDLRLSLQSFQDPIMLQSHHDNHHHNNNNQVLFAGNSSLGYDGGGSGSNLWSEQQQHHQEEHDNGRLHRMMAWNANNVNANAADGGNSGHGAGFVFSTPAPAFGGYGQLFTQRGPLQSSYNPSVRAWIDPSMATANIADHHHHYLSPMIHQASVSGGGFSGFRIPARIQGEEEHDGVSDKPSSASSDSRH