AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032256

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032256.t1 MTR_8g463380 93.622 439 16 5 1 433 1 433 0.0 697
MS.gene032256.t1 MTR_2g090960 43.846 390 137 13 42 424 7 321 1.04e-79 249
MS.gene032256.t1 MTR_4g079580 37.949 390 152 14 42 416 5 319 2.08e-56 189
MS.gene032256.t1 MTR_2g078200 87.059 85 11 0 42 126 3 87 6.25e-48 166
MS.gene032256.t1 MTR_2g078200 48.148 135 57 5 308 433 164 294 2.51e-14 73.6
MS.gene032256.t1 MTR_7g015010 61.842 76 29 0 53 128 51 126 1.12e-25 107
MS.gene032256.t1 MTR_4g109660 63.235 68 25 0 53 120 50 117 2.96e-22 97.1
MS.gene032256.t1 MTR_6g015350 64.706 68 24 0 53 120 43 110 2.34e-21 94.7
MS.gene032256.t1 MTR_3g026050 58.824 68 28 0 53 120 51 118 8.77e-21 94.0
MS.gene032256.t1 MTR_4g111935 43.299 97 52 2 37 132 112 206 8.83e-17 82.0
MS.gene032256.t1 MTR_1g103380 55.738 61 27 0 58 118 200 260 2.13e-16 81.6
MS.gene032256.t1 MTR_6g017055 50.769 65 32 0 53 117 120 184 8.87e-16 79.3
MS.gene032256.t1 MTR_7g018500 56.140 57 25 0 61 117 122 178 8.88e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032256.t1 AT3G15030 48.214 448 141 20 32 433 18 420 1.26e-96 296
MS.gene032256.t1 AT3G15030 48.214 448 141 20 32 433 18 420 1.26e-96 296
MS.gene032256.t1 AT3G15030 48.214 448 141 20 32 433 18 420 1.26e-96 296
MS.gene032256.t1 AT3G15030 48.214 448 141 20 32 433 18 420 1.26e-96 296
MS.gene032256.t1 AT1G53230 46.793 421 150 16 34 433 24 391 5.08e-96 294
MS.gene032256.t1 AT1G53230 46.793 421 150 16 34 433 24 391 5.08e-96 294
MS.gene032256.t1 AT2G31070 44.872 234 71 7 33 256 3 188 1.57e-46 164
MS.gene032256.t1 AT4G18390 70.588 68 20 0 53 120 36 103 3.46e-26 109
MS.gene032256.t1 AT4G18390 70.588 68 20 0 53 120 36 103 3.46e-26 109
MS.gene032256.t1 AT3G02150 68.116 69 22 0 53 121 68 136 1.52e-24 102
MS.gene032256.t1 AT3G02150 68.116 69 22 0 53 121 68 136 3.47e-24 103
MS.gene032256.t1 AT1G30210 64.706 68 24 0 53 120 44 111 1.55e-22 98.2
MS.gene032256.t1 AT1G30210 64.706 68 24 0 53 120 44 111 1.55e-22 98.2
MS.gene032256.t1 AT5G60970 60.294 68 27 0 53 120 57 124 8.66e-22 96.7
MS.gene032256.t1 AT5G08070 58.824 68 28 0 53 120 27 94 3.24e-21 92.8
MS.gene032256.t1 AT5G08070 58.824 68 28 0 53 120 27 94 3.24e-21 92.8
MS.gene032256.t1 AT1G68800 51.471 68 33 0 55 122 108 175 2.17e-19 89.4
MS.gene032256.t1 AT1G68800 51.471 68 33 0 55 122 108 175 2.24e-19 89.7
MS.gene032256.t1 AT1G67260 52.113 71 34 0 54 124 82 152 9.56e-19 87.4
MS.gene032256.t1 AT1G67260 52.113 71 34 0 54 124 82 152 9.56e-19 87.4
MS.gene032256.t1 AT1G67260 52.113 71 34 0 54 124 82 152 1.32e-18 87.4
MS.gene032256.t1 AT3G18550 50.000 64 32 0 55 118 144 207 1.14e-13 72.8
MS.gene032256.t1 AT3G18550 50.000 64 32 0 55 118 144 207 1.14e-13 72.8
MS.gene032256.t1 AT3G18550 50.000 64 32 0 55 118 144 207 1.27e-13 72.8
MS.gene032256.t1 AT3G18550 50.000 64 32 0 55 118 144 207 1.31e-13 72.8

Find 133 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTATACCAGCACGAATTC+AGG 0.286283 8.2:-46469612 MS.gene032256:CDS
GTCTCTGGTGGTGGATTCTC+AGG 0.305280 8.2:-46469638 MS.gene032256:CDS
ATCCATCAGGCTTCTGTCTC+TGG 0.306407 8.2:-46469653 MS.gene032256:CDS
GCACCGGCGTTTGGCGGTTA+TGG 0.331523 8.2:-46469800 MS.gene032256:CDS
GACGTCGTGGGCGTTTCAAC+TGG 0.345584 8.2:+46470178 None:intergenic
TCCGCTGCCGCTGATTCTCT+TGG 0.351084 8.2:+46470738 None:intergenic
AGCGGCAGCGGAGGAGGAGG+AGG 0.358889 8.2:-46470727 MS.gene032256:CDS
TGGTGGCTCTGAAGCATAAT+AGG 0.365493 8.2:+46470055 None:intergenic
ATCAGCGGCAGCGGAGGAGG+AGG 0.374612 8.2:-46470730 MS.gene032256:CDS
GGCAGCGGAGGAGGAGGAGG+AGG 0.377059 8.2:-46470724 MS.gene032256:CDS
AGCCAATCGACTGCTTTACT+TGG 0.379997 8.2:+46470532 None:intergenic
GAGTTCGTCGATAGCAGATT+TGG 0.383791 8.2:+46470498 None:intergenic
ATAACCAAGGGAGGAGTTTC+CGG 0.384724 8.2:+46469991 None:intergenic
GGAAGCAGAGGACGGTTTAT+CGG 0.389422 8.2:+46469565 None:intergenic
AGTACACCGGCACCGGCGTT+TGG 0.392086 8.2:-46469809 MS.gene032256:CDS
TGACTGGTAGAAGCGGTTAC+TGG 0.399280 8.2:+46470364 None:intergenic
GGTCACATTGTCCGTTCAAC+AGG 0.417152 8.2:-46470676 MS.gene032256:CDS
TGTTGTCCACCGGAGATTGC+CGG 0.418132 8.2:+46470389 None:intergenic
CTGTTGCTGTTCCTGTGTGC+TGG 0.422018 8.2:+46470806 None:intergenic
CAGGCTTCTGTCTCTGGTGG+TGG 0.423247 8.2:-46469647 MS.gene032256:CDS
AGAATCAGCGGCAGCGGAGG+AGG 0.424143 8.2:-46470733 MS.gene032256:CDS
GGTTGTTGTTGTTGTGGTGG+TGG 0.431926 8.2:+46470026 None:intergenic
AGAGTCGTACGCGGCGGTCA+CGG 0.437341 8.2:+46470611 None:intergenic
AACTCGCTCACCTCCCTGCT+TGG 0.437620 8.2:-46470480 MS.gene032256:CDS
AGGAGGAGGAGGAGAAATCG+TGG 0.443308 8.2:-46470710 MS.gene032256:CDS
CTATCTCCAACTGTTGTTGT+TGG 0.445345 8.2:+46470287 None:intergenic
GGTAACAGTGGCCATGGTGC+TGG 0.446213 8.2:-46469842 MS.gene032256:CDS
ACGAACTGAAGGATTGTAAC+TGG 0.446835 8.2:+46469745 None:intergenic
GAGTCGTACGCGGCGGTCAC+GGG 0.446966 8.2:+46470612 None:intergenic
TATCTCCAACTGTTGTTGTT+GGG 0.450024 8.2:+46470288 None:intergenic
TTCGCCGGAAACTCCTCCCT+TGG 0.457148 8.2:-46469995 MS.gene032256:CDS
TGTTGTGGTGGTGGTTGTCA+TGG 0.458311 8.2:+46470035 None:intergenic
ACAGAAGCCTGATGGATCAT+TGG 0.470560 8.2:+46469659 None:intergenic
ACACCGGCACCGGCGTTTGG+CGG 0.478717 8.2:-46469806 MS.gene032256:CDS
GTAGCTGTGGGGTTCCAAGC+AGG 0.479135 8.2:+46470466 None:intergenic
AGAGGCGGAGATCTTGCTGC+TGG 0.481925 8.2:+46470098 None:intergenic
TAATGCCGCTGAGCGGTGAC+TGG 0.483807 8.2:+46470348 None:intergenic
ATATTCGCCGTCGCCATTGA+AGG 0.485547 8.2:+46469710 None:intergenic
ACAATCCTTCAGTTCGTGCT+TGG 0.487224 8.2:-46469739 MS.gene032256:CDS
CGTATACCAGCACGAATTCA+GGG 0.492711 8.2:-46469611 MS.gene032256:CDS
TCCTTCCCAACAACAACAGT+TGG 0.493806 8.2:-46470293 MS.gene032256:CDS
GTCGTTCGAGAGAGTAAATC+CGG 0.495710 8.2:+46470133 None:intergenic
TCTTTCGCCAATGATCCATC+AGG 0.495748 8.2:-46469666 MS.gene032256:CDS
TCCAAGAGAATCAGCGGCAG+CGG 0.496376 8.2:-46470739 MS.gene032256:CDS
GCGGTCACGGGGACCTTTCG+CGG 0.503457 8.2:+46470624 None:intergenic
AAGAGAATCAGCGGCAGCGG+AGG 0.505536 8.2:-46470736 MS.gene032256:CDS
TCCAACTGTTGTTGTTGGGA+AGG 0.512262 8.2:+46470292 None:intergenic
GCACTTGGTTGTTGTTGTTG+TGG 0.512670 8.2:+46470020 None:intergenic
AATGTTTCACCGGCAATCTC+CGG 0.513568 8.2:-46470398 MS.gene032256:CDS
CTTGGTTGTTGTTGTTGTGG+TGG 0.513896 8.2:+46470023 None:intergenic
GAAGGAAATTGTTGTTCTTG+TGG 0.514512 8.2:+46470310 None:intergenic
GGAGAAATCGTGGAGGTACA+AGG 0.518138 8.2:-46470700 MS.gene032256:CDS
GGTTATGACGGTGGTGGAAG+TGG 0.521829 8.2:-46469974 MS.gene032256:CDS
TAACCGCCAAACGCCGGTGC+CGG 0.524727 8.2:+46469803 None:intergenic
TCCCTTGGTTATGACGGTGG+TGG 0.526752 8.2:-46469980 MS.gene032256:CDS
AACTCCTCCCTTGGTTATGA+CGG 0.526876 8.2:-46469986 MS.gene032256:CDS
AGTTTCCGGCGAAAAGCACT+TGG 0.528802 8.2:+46470005 None:intergenic
TCTTCACCCTGAATTCGTGC+TGG 0.532864 8.2:+46469605 None:intergenic
GGTCTTTGCGTCCTGTTGAA+CGG 0.539979 8.2:+46470665 None:intergenic
TGCTTGGATAGATCCTTCAA+TGG 0.540229 8.2:-46469723 MS.gene032256:CDS
TGTGTTCAGTACACCGGCAC+CGG 0.543423 8.2:-46469816 MS.gene032256:CDS
CGGCAGATTGCATAGAATGA+TGG 0.543570 8.2:-46469906 MS.gene032256:CDS
AGGATTGTAACTGGACTGAA+GGG 0.547857 8.2:+46469754 None:intergenic
CACCAGAGACAGAAGCCTGA+TGG 0.548735 8.2:+46469651 None:intergenic
GTTACTGGATTGTTGTCCAC+CGG 0.554367 8.2:+46470379 None:intergenic
GGCCAAGTAAAGCAGTCGAT+TGG 0.560622 8.2:-46470534 MS.gene032256:CDS
ACAACACACGACCAGCACAC+AGG 0.563376 8.2:-46470817 MS.gene032256:CDS
GATTGCATAGAATGATGGCT+TGG 0.567846 8.2:-46469901 MS.gene032256:CDS
GCGAGAGTCGGAGGAAGCAG+AGG 0.568701 8.2:+46469553 None:intergenic
TAGCTGTGGGGTTCCAAGCA+GGG 0.568717 8.2:+46470467 None:intergenic
AGTAAAGTATGCACCGCGAA+AGG 0.570958 8.2:-46470637 MS.gene032256:CDS
AAACAAATGAAATGTTTCAC+CGG 0.571359 8.2:-46470408 MS.gene032256:CDS
GCCGCTGATTCTCTTGGACG+AGG 0.572452 8.2:+46470744 None:intergenic
CATCAAGAGGAACATGATAA+CGG 0.573201 8.2:-46469926 MS.gene032256:CDS
AAGGATTGTAACTGGACTGA+AGG 0.573483 8.2:+46469753 None:intergenic
AGTCGTACGCGGCGGTCACG+GGG 0.577670 8.2:+46470613 None:intergenic
GTAGAATTGAATCGCAGTGT+GGG 0.579117 8.2:+46470585 None:intergenic
GAGGCGGAGATCTTGCTGCT+GGG 0.582996 8.2:+46470099 None:intergenic
AAGATCGTCTCGGCTATGAC+AGG 0.584840 8.2:-46470555 MS.gene032256:CDS
CAATCAATGGCGAGAGTCGG+AGG 0.587240 8.2:+46469544 None:intergenic
CGTAGAATTGAATCGCAGTG+TGG 0.587501 8.2:+46470584 None:intergenic
CATCAGGCTTCTGTCTCTGG+TGG 0.590853 8.2:-46469650 MS.gene032256:CDS
TCTATCCAAGCACGAACTGA+AGG 0.592530 8.2:+46469734 None:intergenic
AGTAGTAGCAGTGGGAAACA+TGG 0.592595 8.2:-46470256 MS.gene032256:CDS
GGAAATTGTTGTTCTTGTGG+TGG 0.596044 8.2:+46470313 None:intergenic
ATGCTGCTGCTCAGACCACA+AGG 0.600677 8.2:+46469946 None:intergenic
TGTGGTGGTGGTTGTCATGG+TGG 0.600873 8.2:+46470038 None:intergenic
TTCCAATCAATGGCGAGAGT+CGG 0.602863 8.2:+46469541 None:intergenic
AGCACGAATTCAGGGTGAAG+AGG 0.603117 8.2:-46469603 MS.gene032256:CDS
GATGGTGGTAACAGTGGCCA+TGG 0.608651 8.2:-46469848 MS.gene032256:CDS
TGACCATAACCGCCAAACGC+CGG 0.611880 8.2:+46469797 None:intergenic
GATAGATCCTTCAATGGCGA+CGG 0.613580 8.2:-46469717 MS.gene032256:CDS
GACTACAACAGTAGTAGCAG+TGG 0.615455 8.2:-46470265 MS.gene032256:CDS
GTTTCACCGGCAATCTCCGG+TGG 0.616152 8.2:-46470395 MS.gene032256:CDS
TACGACGTGCAAGATCGTCT+CGG 0.617162 8.2:-46470565 MS.gene032256:CDS
GTTAATGCTAATACTGCAGA+TGG 0.619281 8.2:-46469866 MS.gene032256:CDS
CGGTGCATACTTTACTGTGA+CGG 0.619367 8.2:+46470644 None:intergenic
GTCTGAGCAGCAGCATCAAG+AGG 0.619394 8.2:-46469939 MS.gene032256:CDS
TTCCACCACCGTCATAACCA+AGG 0.620568 8.2:+46469978 None:intergenic
ATAACTGTGAAACGACGTCG+TGG 0.622223 8.2:+46470165 None:intergenic
TTGGCGAAAGATAGTGATGA+TGG 0.622368 8.2:+46469678 None:intergenic
CTTGAAAAGATTGCAATGAG+AGG 0.622677 8.2:+46470080 None:intergenic
GAGTCGGAGGAAGCAGAGGA+CGG 0.623160 8.2:+46469557 None:intergenic
ACCTCGTCCAAGAGAATCAG+CGG 0.626058 8.2:-46470745 MS.gene032256:CDS
TGTTGAAGAACATGTAGCTG+TGG 0.626291 8.2:+46470453 None:intergenic
GTTGAAGAACATGTAGCTGT+GGG 0.630226 8.2:+46470454 None:intergenic
TCCTCCCTTGGTTATGACGG+TGG 0.633414 8.2:-46469983 MS.gene032256:CDS
TGGATTTGTGTTCAGTACAC+CGG 0.633611 8.2:-46469822 MS.gene032256:CDS
GAAAAGATTGCAATGAGAGG+CGG 0.637117 8.2:+46470083 None:intergenic
TCACAGTTATCAACAACCGC+CGG 0.637674 8.2:-46470152 MS.gene032256:CDS
GGATTTACTCTCTCGAACGA+CGG 0.642953 8.2:-46470131 MS.gene032256:CDS
GCGGAGATCTTGCTGCTGGG+TGG 0.647953 8.2:+46470102 None:intergenic
TCCACCACCGTCATAACCAA+GGG 0.649518 8.2:+46469979 None:intergenic
ACTGCAGATGGTGGTAACAG+TGG 0.650408 8.2:-46469854 MS.gene032256:CDS
GTGGAAGTGGAAGCACCTTG+TGG 0.652297 8.2:-46469961 MS.gene032256:CDS
ACTACAACAGTAGTAGCAGT+GGG 0.659751 8.2:-46470264 MS.gene032256:CDS
TAACTGTGAAACGACGTCGT+GGG 0.673437 8.2:+46470166 None:intergenic
TGAGCGGTGACTGGTAGAAG+CGG 0.674706 8.2:+46470357 None:intergenic
ACCACCGTCATAACCAAGGG+AGG 0.674731 8.2:+46469982 None:intergenic
TTCGCTCTAATGCCGCTGAG+CGG 0.675852 8.2:+46470341 None:intergenic
GGATTGTAACTGGACTGAAG+GGG 0.704803 8.2:+46469755 None:intergenic
TGAACACAAATCCAGCACCA+TGG 0.705786 8.2:+46469831 None:intergenic
AATGCTAATACTGCAGATGG+TGG 0.705808 8.2:-46469863 MS.gene032256:CDS
TTCTACCAGTCACCGCTCAG+CGG 0.712108 8.2:-46470353 MS.gene032256:CDS
CTGTGGGGTTCCAAGCAGGG+AGG 0.716198 8.2:+46470470 None:intergenic
TGTGGGCTGAGAGTCGTACG+CGG 0.717449 8.2:+46470602 None:intergenic
AGGAGGAGGAGAAATCGTGG+AGG 0.725082 8.2:-46470707 MS.gene032256:CDS
TTTACTCTCTCGAACGACGG+CGG 0.728543 8.2:-46470128 MS.gene032256:CDS
GTTCGAGAGAGTAAATCCGG+CGG 0.739877 8.2:+46470136 None:intergenic
GAAATCGTGGAGGTACAAGG+AGG 0.742186 8.2:-46470697 MS.gene032256:CDS
GGGCTGAGAGTCGTACGCGG+CGG 0.755550 8.2:+46470605 None:intergenic
TTGAAGAACATGTAGCTGTG+GGG 0.767979 8.2:+46470455 None:intergenic
CAGGGTGAAGAGGAACACGA+CGG 0.776987 8.2:-46469593 MS.gene032256:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAACAAATGAAATGTTTCAC+CGG - chr8.2:46469965-46469984 MS.gene032256:CDS 25.0%
CATCAAGAGGAACATGATAA+CGG - chr8.2:46470447-46470466 MS.gene032256:CDS 35.0%
CTTGAAAAGATTGCAATGAG+AGG + chr8.2:46470296-46470315 None:intergenic 35.0%
GTTAATGCTAATACTGCAGA+TGG - chr8.2:46470507-46470526 MS.gene032256:CDS 35.0%
! GAAGGAAATTGTTGTTCTTG+TGG + chr8.2:46470066-46470085 None:intergenic 35.0%
! TATCTCCAACTGTTGTTGTT+GGG + chr8.2:46470088-46470107 None:intergenic 35.0%
!!! ATGGTCAGTTTTTTACTCAG+AGG - chr8.2:46470592-46470611 MS.gene032256:CDS 35.0%
!!! TGGTCAGTTTTTTACTCAGA+GGG - chr8.2:46470593-46470612 MS.gene032256:CDS 35.0%
AAGGATTGTAACTGGACTGA+AGG + chr8.2:46470623-46470642 None:intergenic 40.0%
AATGCTAATACTGCAGATGG+TGG - chr8.2:46470510-46470529 MS.gene032256:CDS 40.0%
ACGAACTGAAGGATTGTAAC+TGG + chr8.2:46470631-46470650 None:intergenic 40.0%
ACTACAACAGTAGTAGCAGT+GGG - chr8.2:46470109-46470128 MS.gene032256:CDS 40.0%
AGGATTGTAACTGGACTGAA+GGG + chr8.2:46470622-46470641 None:intergenic 40.0%
CTATCTCCAACTGTTGTTGT+TGG + chr8.2:46470089-46470108 None:intergenic 40.0%
GAAAAGATTGCAATGAGAGG+CGG + chr8.2:46470293-46470312 None:intergenic 40.0%
GATTGCATAGAATGATGGCT+TGG - chr8.2:46470472-46470491 MS.gene032256:CDS 40.0%
GTAGAATTGAATCGCAGTGT+GGG + chr8.2:46469791-46469810 None:intergenic 40.0%
GTTGAAGAACATGTAGCTGT+GGG + chr8.2:46469922-46469941 None:intergenic 40.0%
TGCTTGGATAGATCCTTCAA+TGG - chr8.2:46470650-46470669 MS.gene032256:CDS 40.0%
TGGATTTGTGTTCAGTACAC+CGG - chr8.2:46470551-46470570 MS.gene032256:CDS 40.0%
TGTTGAAGAACATGTAGCTG+TGG + chr8.2:46469923-46469942 None:intergenic 40.0%
TTGAAGAACATGTAGCTGTG+GGG + chr8.2:46469921-46469940 None:intergenic 40.0%
TTGGCGAAAGATAGTGATGA+TGG + chr8.2:46470698-46470717 None:intergenic 40.0%
! GGAAATTGTTGTTCTTGTGG+TGG + chr8.2:46470063-46470082 None:intergenic 40.0%
! GGGAAACATGGTTTTCTTCA+AGG - chr8.2:46470129-46470148 MS.gene032256:CDS 40.0%
!!! GGTCAGTTTTTTACTCAGAG+GGG - chr8.2:46470594-46470613 MS.gene032256:CDS 40.0%
AACTCCTCCCTTGGTTATGA+CGG - chr8.2:46470387-46470406 MS.gene032256:CDS 45.0%
AATGTTTCACCGGCAATCTC+CGG - chr8.2:46469975-46469994 MS.gene032256:CDS 45.0%
ACAATCCTTCAGTTCGTGCT+TGG - chr8.2:46470634-46470653 MS.gene032256:CDS 45.0%
AGCCAATCGACTGCTTTACT+TGG + chr8.2:46469844-46469863 None:intergenic 45.0%
AGTAAAGTATGCACCGCGAA+AGG - chr8.2:46469736-46469755 MS.gene032256:CDS 45.0%
AGTAGTAGCAGTGGGAAACA+TGG - chr8.2:46470117-46470136 MS.gene032256:CDS 45.0%
ATAACCAAGGGAGGAGTTTC+CGG + chr8.2:46470385-46470404 None:intergenic 45.0%
ATAACTGTGAAACGACGTCG+TGG + chr8.2:46470211-46470230 None:intergenic 45.0%
CGGCAGATTGCATAGAATGA+TGG - chr8.2:46470467-46470486 MS.gene032256:CDS 45.0%
CGGTGCATACTTTACTGTGA+CGG + chr8.2:46469732-46469751 None:intergenic 45.0%
CGTAGAATTGAATCGCAGTG+TGG + chr8.2:46469792-46469811 None:intergenic 45.0%
CGTATACCAGCACGAATTCA+GGG - chr8.2:46470762-46470781 MS.gene032256:CDS 45.0%
GACTACAACAGTAGTAGCAG+TGG - chr8.2:46470108-46470127 MS.gene032256:CDS 45.0%
GAGTTCGTCGATAGCAGATT+TGG + chr8.2:46469878-46469897 None:intergenic 45.0%
GATAGATCCTTCAATGGCGA+CGG - chr8.2:46470656-46470675 MS.gene032256:CDS 45.0%
GGATTGTAACTGGACTGAAG+GGG + chr8.2:46470621-46470640 None:intergenic 45.0%
GGATTTACTCTCTCGAACGA+CGG - chr8.2:46470242-46470261 MS.gene032256:CDS 45.0%
GTCGTTCGAGAGAGTAAATC+CGG + chr8.2:46470243-46470262 None:intergenic 45.0%
GTTACTGGATTGTTGTCCAC+CGG + chr8.2:46469997-46470016 None:intergenic 45.0%
TAACTGTGAAACGACGTCGT+GGG + chr8.2:46470210-46470229 None:intergenic 45.0%
TCACAGTTATCAACAACCGC+CGG - chr8.2:46470221-46470240 MS.gene032256:CDS 45.0%
TCCTTCCCAACAACAACAGT+TGG - chr8.2:46470080-46470099 MS.gene032256:CDS 45.0%
TCGTATACCAGCACGAATTC+AGG - chr8.2:46470761-46470780 MS.gene032256:CDS 45.0%
TCTATCCAAGCACGAACTGA+AGG + chr8.2:46470642-46470661 None:intergenic 45.0%
TCTTTCGCCAATGATCCATC+AGG - chr8.2:46470707-46470726 MS.gene032256:CDS 45.0%
TGAACACAAATCCAGCACCA+TGG + chr8.2:46470545-46470564 None:intergenic 45.0%
! AACAACCAAGTGCTTTTCGC+CGG - chr8.2:46470363-46470382 MS.gene032256:CDS 45.0%
! ACAGAAGCCTGATGGATCAT+TGG + chr8.2:46470717-46470736 None:intergenic 45.0%
! CTTGGTTGTTGTTGTTGTGG+TGG + chr8.2:46470353-46470372 None:intergenic 45.0%
! GCACTTGGTTGTTGTTGTTG+TGG + chr8.2:46470356-46470375 None:intergenic 45.0%
! TCCAACTGTTGTTGTTGGGA+AGG + chr8.2:46470084-46470103 None:intergenic 45.0%
!! TGGTGGCTCTGAAGCATAAT+AGG + chr8.2:46470321-46470340 None:intergenic 45.0%
AAGATCGTCTCGGCTATGAC+AGG - chr8.2:46469818-46469837 MS.gene032256:CDS 50.0%
ACCTCGTCCAAGAGAATCAG+CGG - chr8.2:46469628-46469647 MS.gene032256:CDS 50.0%
AGCACGAATTCAGGGTGAAG+AGG - chr8.2:46470770-46470789 MS.gene032256:CDS 50.0%
ATATTCGCCGTCGCCATTGA+AGG + chr8.2:46470666-46470685 None:intergenic 50.0%
ATCCATCAGGCTTCTGTCTC+TGG - chr8.2:46470720-46470739 MS.gene032256:CDS 50.0%
GGAAGCAGAGGACGGTTTAT+CGG + chr8.2:46470811-46470830 None:intergenic 50.0%
GGCCAAGTAAAGCAGTCGAT+TGG - chr8.2:46469839-46469858 MS.gene032256:CDS 50.0%
GGTCACATTGTCCGTTCAAC+AGG - chr8.2:46469697-46469716 MS.gene032256:CDS 50.0%
GGTCTTTGCGTCCTGTTGAA+CGG + chr8.2:46469711-46469730 None:intergenic 50.0%
GTTCGAGAGAGTAAATCCGG+CGG + chr8.2:46470240-46470259 None:intergenic 50.0%
TACGACGTGCAAGATCGTCT+CGG - chr8.2:46469808-46469827 MS.gene032256:CDS 50.0%
TCCACCACCGTCATAACCAA+GGG + chr8.2:46470397-46470416 None:intergenic 50.0%
TCTTCACCCTGAATTCGTGC+TGG + chr8.2:46470771-46470790 None:intergenic 50.0%
TGACTGGTAGAAGCGGTTAC+TGG + chr8.2:46470012-46470031 None:intergenic 50.0%
TTCCACCACCGTCATAACCA+AGG + chr8.2:46470398-46470417 None:intergenic 50.0%
TTTACTCTCTCGAACGACGG+CGG - chr8.2:46470245-46470264 MS.gene032256:CDS 50.0%
! ACTGCAGATGGTGGTAACAG+TGG - chr8.2:46470519-46470538 MS.gene032256:CDS 50.0%
! GAAATCGTGGAGGTACAAGG+AGG - chr8.2:46469676-46469695 MS.gene032256:CDS 50.0%
! GGAGAAATCGTGGAGGTACA+AGG - chr8.2:46469673-46469692 MS.gene032256:CDS 50.0%
! GGTTGTTGTTGTTGTGGTGG+TGG + chr8.2:46470350-46470369 None:intergenic 50.0%
!! AGTTTCCGGCGAAAAGCACT+TGG + chr8.2:46470371-46470390 None:intergenic 50.0%
!! TGTTGTGGTGGTGGTTGTCA+TGG + chr8.2:46470341-46470360 None:intergenic 50.0%
ACAACACACGACCAGCACAC+AGG - chr8.2:46469556-46469575 MS.gene032256:CDS 55.0%
ACCACCGTCATAACCAAGGG+AGG + chr8.2:46470394-46470413 None:intergenic 55.0%
AGGAGGAGGAGAAATCGTGG+AGG - chr8.2:46469666-46469685 MS.gene032256:CDS 55.0%
AGGAGGAGGAGGAGAAATCG+TGG - chr8.2:46469663-46469682 MS.gene032256:CDS 55.0%
ATGCTGCTGCTCAGACCACA+AGG + chr8.2:46470430-46470449 None:intergenic 55.0%
CACCAGAGACAGAAGCCTGA+TGG + chr8.2:46470725-46470744 None:intergenic 55.0%
CAGGGTGAAGAGGAACACGA+CGG - chr8.2:46470780-46470799 MS.gene032256:CDS 55.0%
CATCAGGCTTCTGTCTCTGG+TGG - chr8.2:46470723-46470742 MS.gene032256:CDS 55.0%
GTCTGAGCAGCAGCATCAAG+AGG - chr8.2:46470434-46470453 MS.gene032256:CDS 55.0%
TAGCTGTGGGGTTCCAAGCA+GGG + chr8.2:46469909-46469928 None:intergenic 55.0%
TCCAAGAGAATCAGCGGCAG+CGG - chr8.2:46469634-46469653 MS.gene032256:CDS 55.0%
TCCCTTGGTTATGACGGTGG+TGG - chr8.2:46470393-46470412 MS.gene032256:CDS 55.0%
TCCTCCCTTGGTTATGACGG+TGG - chr8.2:46470390-46470409 MS.gene032256:CDS 55.0%
TGACCATAACCGCCAAACGC+CGG + chr8.2:46470579-46470598 None:intergenic 55.0%
TGAGCGGTGACTGGTAGAAG+CGG + chr8.2:46470019-46470038 None:intergenic 55.0%
TGTGTTCAGTACACCGGCAC+CGG - chr8.2:46470557-46470576 MS.gene032256:CDS 55.0%
TGTTGTCCACCGGAGATTGC+CGG + chr8.2:46469987-46470006 None:intergenic 55.0%
TTCGCTCTAATGCCGCTGAG+CGG + chr8.2:46470035-46470054 None:intergenic 55.0%
TTCTACCAGTCACCGCTCAG+CGG - chr8.2:46470020-46470039 MS.gene032256:CDS 55.0%
! CTGTTGCTGTTCCTGTGTGC+TGG + chr8.2:46469570-46469589 None:intergenic 55.0%
! GATGGTGGTAACAGTGGCCA+TGG - chr8.2:46470525-46470544 MS.gene032256:CDS 55.0%
!! GGTTATGACGGTGGTGGAAG+TGG - chr8.2:46470399-46470418 MS.gene032256:CDS 55.0%
!! GTCTCTGGTGGTGGATTCTC+AGG - chr8.2:46470735-46470754 MS.gene032256:CDS 55.0%
!! GTGGAAGTGGAAGCACCTTG+TGG - chr8.2:46470412-46470431 MS.gene032256:CDS 55.0%
!! TGTGGTGGTGGTTGTCATGG+TGG + chr8.2:46470338-46470357 None:intergenic 55.0%
AACTCGCTCACCTCCCTGCT+TGG - chr8.2:46469893-46469912 MS.gene032256:CDS 60.0%
AAGAGAATCAGCGGCAGCGG+AGG - chr8.2:46469637-46469656 MS.gene032256:CDS 60.0%
AGAGGCGGAGATCTTGCTGC+TGG + chr8.2:46470278-46470297 None:intergenic 60.0%
CAGGCTTCTGTCTCTGGTGG+TGG - chr8.2:46470726-46470745 MS.gene032256:CDS 60.0%
GACGTCGTGGGCGTTTCAAC+TGG + chr8.2:46470198-46470217 None:intergenic 60.0%
GAGGCGGAGATCTTGCTGCT+GGG + chr8.2:46470277-46470296 None:intergenic 60.0%
GAGTCGGAGGAAGCAGAGGA+CGG + chr8.2:46470819-46470838 None:intergenic 60.0%
GGTAACAGTGGCCATGGTGC+TGG - chr8.2:46470531-46470550 MS.gene032256:CDS 60.0%
GTAGCTGTGGGGTTCCAAGC+AGG + chr8.2:46469910-46469929 None:intergenic 60.0%
GTTTCACCGGCAATCTCCGG+TGG - chr8.2:46469978-46469997 MS.gene032256:CDS 60.0%
TAATGCCGCTGAGCGGTGAC+TGG + chr8.2:46470028-46470047 None:intergenic 60.0%
TTCGCCGGAAACTCCTCCCT+TGG - chr8.2:46470378-46470397 MS.gene032256:CDS 60.0%
! TGTGGGCTGAGAGTCGTACG+CGG + chr8.2:46469774-46469793 None:intergenic 60.0%
!! GCCGCTGATTCTCTTGGACG+AGG + chr8.2:46469632-46469651 None:intergenic 60.0%
!! TCCGCTGCCGCTGATTCTCT+TGG + chr8.2:46469638-46469657 None:intergenic 60.0%
AGAATCAGCGGCAGCGGAGG+AGG - chr8.2:46469640-46469659 MS.gene032256:CDS 65.0%
AGAGTCGTACGCGGCGGTCA+CGG + chr8.2:46469765-46469784 None:intergenic 65.0%
CTGTGGGGTTCCAAGCAGGG+AGG + chr8.2:46469906-46469925 None:intergenic 65.0%
GCGAGAGTCGGAGGAAGCAG+AGG + chr8.2:46470823-46470842 None:intergenic 65.0%
GCGGAGATCTTGCTGCTGGG+TGG + chr8.2:46470274-46470293 None:intergenic 65.0%
TAACCGCCAAACGCCGGTGC+CGG + chr8.2:46470573-46470592 None:intergenic 65.0%
TGCTGCTGCTGCTGCTGCTG+TGG + chr8.2:46469946-46469965 None:intergenic 65.0%
! GCACCGGCGTTTGGCGGTTA+TGG - chr8.2:46470573-46470592 MS.gene032256:CDS 65.0%
!! AGTACACCGGCACCGGCGTT+TGG - chr8.2:46470564-46470583 MS.gene032256:CDS 65.0%
AGCGGAGGAGGAGGAGGAGG+AGG - chr8.2:46469652-46469671 MS.gene032256:CDS 70.0%
AGTCGTACGCGGCGGTCACG+GGG + chr8.2:46469763-46469782 None:intergenic 70.0%
ATCAGCGGCAGCGGAGGAGG+AGG - chr8.2:46469643-46469662 MS.gene032256:CDS 70.0%
GAGTCGTACGCGGCGGTCAC+GGG + chr8.2:46469764-46469783 None:intergenic 70.0%
GCGGTCACGGGGACCTTTCG+CGG + chr8.2:46469752-46469771 None:intergenic 70.0%
GGGCTGAGAGTCGTACGCGG+CGG + chr8.2:46469771-46469790 None:intergenic 70.0%
!! ACACCGGCACCGGCGTTTGG+CGG - chr8.2:46470567-46470586 MS.gene032256:CDS 70.0%
AGCGGCAGCGGAGGAGGAGG+AGG - chr8.2:46469646-46469665 MS.gene032256:CDS 75.0%
GGCAGCGGAGGAGGAGGAGG+AGG - chr8.2:46469649-46469668 MS.gene032256:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 46469547 46470848 46469547 ID=MS.gene032256
chr8.2 mRNA 46469547 46470848 46469547 ID=MS.gene032256.t1;Parent=MS.gene032256
chr8.2 exon 46469547 46470848 46469547 ID=MS.gene032256.t1.exon1;Parent=MS.gene032256.t1
chr8.2 CDS 46469547 46470848 46469547 ID=cds.MS.gene032256.t1;Parent=MS.gene032256.t1
Gene Sequence

>MS.gene032256

ATGGGAGAAACAACACACGACCAGCACACAGGAACAGCAACAGCAACAGCTACATCAACATCAACAAAAACAGCAGCAACAACCTCGTCCAAGAGAATCAGCGGCAGCGGAGGAGGAGGAGGAGGAGGAGAAATCGTGGAGGTACAAGGAGGTCACATTGTCCGTTCAACAGGACGCAAAGACCGTCACAGTAAAGTATGCACCGCGAAAGGTCCCCGTGACCGCCGCGTACGACTCTCAGCCCACACTGCGATTCAATTCTACGACGTGCAAGATCGTCTCGGCTATGACAGGCCAAGTAAAGCAGTCGATTGGCTCATTAAAAAAGCCAAATCTGCTATCGACGAACTCGCTCACCTCCCTGCTTGGAACCCCACAGCTACATGTTCTTCAACACCACAGCAGCAGCAGCAGCAGCAAACAAATGAAATGTTTCACCGGCAATCTCCGGTGGACAACAATCCAGTAACCGCTTCTACCAGTCACCGCTCAGCGGCATTAGAGCGAAGAATGCCACCACAAGAACAACAATTTCCTTCCCAACAACAACAGTTGGAGATAGACTACAACAGTAGTAGCAGTGGGAAACATGGTTTTCTTCAAGGCTCGTTAGACACTGACATTGCTGATACTATTAAGTCTTTTTTTCCAGTTGAAACGCCCACGACGTCGTTTCACAGTTATCAACAACCGCCGGATTTACTCTCTCGAACGACGGCGGCAACCACCCAGCAGCAAGATCTCCGCCTCTCATTGCAATCTTTTCAAGATCCTATTATGCTTCAGAGCCACCATGACAACCACCACCACAACAACAACAACCAAGTGCTTTTCGCCGGAAACTCCTCCCTTGGTTATGACGGTGGTGGAAGTGGAAGCACCTTGTGGTCTGAGCAGCAGCATCAAGAGGAACATGATAACGGCAGATTGCATAGAATGATGGCTTGGAATGCTAATAATGTTAATGCTAATACTGCAGATGGTGGTAACAGTGGCCATGGTGCTGGATTTGTGTTCAGTACACCGGCACCGGCGTTTGGCGGTTATGGTCAGTTTTTTACTCAGAGGGGACCCCTTCAGTCCAGTTACAATCCTTCAGTTCGTGCTTGGATAGATCCTTCAATGGCGACGGCGAATATCGCTGATCACCATCATCACTATCTTTCGCCAATGATCCATCAGGCTTCTGTCTCTGGTGGTGGATTCTCAGGCTTTCGTATACCAGCACGAATTCAGGGTGAAGAGGAACACGACGGCGTATCCGATAAACCGTCCTCTGCTTCCTCCGACTCTCGCCATTGA
Protein sequence

>MS.gene032256.t1

MGETTHDQHTGTATATATSTSTKTAATTSSKRISGSGGGGGGGEIVEVQGGHIVRSTGRKDRHSKVCTAKGPRDRRVRLSAHTAIQFYDVQDRLGYDRPSKAVDWLIKKAKSAIDELAHLPAWNPTATCSSTPQQQQQQQTNEMFHRQSPVDNNPVTASTSHRSAALERRMPPQEQQFPSQQQQLEIDYNSSSSGKHGFLQGSLDTDIADTIKSFFPVETPTTSFHSYQQPPDLLSRTTAATTQQQDLRLSLQSFQDPIMLQSHHDNHHHNNNNQVLFAGNSSLGYDGGGSGSTLWSEQQHQEEHDNGRLHRMMAWNANNVNANTADGGNSGHGAGFVFSTPAPAFGGYGQFFTQRGPLQSSYNPSVRAWIDPSMATANIADHHHHYLSPMIHQASVSGGGFSGFRIPARIQGEEEHDGVSDKPSSASSDSRH