Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009229.t1 | XP_003628618.1 | 99 | 395 | 3 | 1 | 1 | 394 | 1 | 395 | 1.60E-223 | 785 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009229.t1 | P52902 | 82.5 | 377 | 66 | 0 | 18 | 394 | 21 | 397 | 1.9e-185 | 649.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009229.t1 | G7LEP9 | 99.0 | 395 | 3 | 1 | 1 | 394 | 1 | 395 | 1.1e-223 | 785.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76034 | MS.gene009229 | PPI |
| MS.gene009229 | MS.gene049277 | PPI |
| MS.gene009229 | MS.gene06396 | PPI |
| MS.gene074258 | MS.gene009229 | PPI |
| MS.gene009229 | MS.gene054963 | PPI |
| MS.gene013304 | MS.gene009229 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009229.t1 | MTR_8g062670 | 98.987 | 395 | 3 | 1 | 1 | 394 | 1 | 395 | 0.0 | 818 |
| MS.gene009229.t1 | MTR_5g036600 | 82.984 | 382 | 63 | 1 | 13 | 394 | 16 | 395 | 0.0 | 654 |
| MS.gene009229.t1 | MTR_8g024310 | 35.629 | 421 | 226 | 9 | 6 | 388 | 3 | 416 | 3.22e-81 | 255 |
| MS.gene009229.t1 | MTR_3g088845 | 29.231 | 325 | 220 | 4 | 57 | 375 | 31 | 351 | 1.23e-42 | 153 |
| MS.gene009229.t1 | MTR_3g088845 | 29.231 | 325 | 220 | 4 | 57 | 375 | 119 | 439 | 5.27e-42 | 154 |
| MS.gene009229.t1 | MTR_3g096390 | 28.994 | 338 | 218 | 7 | 49 | 373 | 90 | 418 | 1.01e-40 | 150 |
| MS.gene009229.t1 | MTR_3g096390 | 28.063 | 253 | 160 | 7 | 49 | 288 | 90 | 333 | 3.34e-24 | 102 |
| MS.gene009229.t1 | MTR_3g088845 | 25.683 | 183 | 129 | 2 | 57 | 236 | 119 | 297 | 3.66e-17 | 82.0 |
| MS.gene009229.t1 | MTR_8g075470 | 27.074 | 229 | 139 | 7 | 99 | 311 | 61 | 277 | 8.80e-15 | 75.5 |
| MS.gene009229.t1 | MTR_7g118200 | 38.095 | 84 | 47 | 3 | 223 | 301 | 5 | 88 | 1.95e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009229.t1 | AT1G24180 | 77.834 | 397 | 81 | 4 | 1 | 394 | 1 | 393 | 0.0 | 647 |
| MS.gene009229.t1 | AT1G59900 | 76.396 | 394 | 88 | 3 | 1 | 394 | 1 | 389 | 0.0 | 645 |
| MS.gene009229.t1 | AT1G59900 | 76.579 | 380 | 84 | 3 | 1 | 380 | 1 | 375 | 0.0 | 624 |
| MS.gene009229.t1 | AT1G01090 | 37.306 | 386 | 221 | 9 | 20 | 388 | 36 | 417 | 7.58e-77 | 244 |
| MS.gene009229.t1 | AT5G09300 | 28.399 | 331 | 217 | 6 | 54 | 373 | 49 | 370 | 6.38e-42 | 152 |
| MS.gene009229.t1 | AT5G09300 | 28.399 | 331 | 217 | 6 | 54 | 373 | 120 | 441 | 1.95e-41 | 152 |
| MS.gene009229.t1 | AT1G21400 | 31.148 | 305 | 196 | 5 | 99 | 394 | 72 | 371 | 1.65e-40 | 147 |
| MS.gene009229.t1 | AT1G21400 | 31.148 | 305 | 196 | 5 | 99 | 394 | 72 | 371 | 1.65e-40 | 147 |
| MS.gene009229.t1 | AT1G21400 | 31.148 | 305 | 196 | 5 | 99 | 394 | 89 | 388 | 2.70e-40 | 147 |
| MS.gene009229.t1 | AT1G21400 | 31.148 | 305 | 196 | 5 | 99 | 394 | 164 | 463 | 3.57e-40 | 149 |
| MS.gene009229.t1 | AT1G21400 | 31.148 | 305 | 196 | 5 | 99 | 394 | 216 | 515 | 4.98e-40 | 149 |
| MS.gene009229.t1 | AT5G09300 | 27.935 | 247 | 158 | 6 | 54 | 289 | 120 | 357 | 4.08e-25 | 105 |
| MS.gene009229.t1 | AT5G34780 | 34.043 | 188 | 109 | 5 | 194 | 373 | 31 | 211 | 1.04e-21 | 95.9 |
| MS.gene009229.t1 | AT5G09300 | 27.419 | 186 | 128 | 2 | 54 | 236 | 120 | 301 | 5.91e-20 | 90.5 |
| MS.gene009229.t1 | AT1G21400 | 28.378 | 148 | 103 | 1 | 99 | 243 | 216 | 363 | 6.98e-17 | 81.6 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGATCTTCCTGCTATTT+TGG | 0.127087 | 8.1:-42322186 | MS.gene009229:CDS |
| TTTACCAATGTATATGTTAA+AGG | 0.140584 | 8.1:-42314859 | MS.gene009229:CDS |
| CTTGGAGTTGGATTGGCTTT+TGG | 0.188485 | 8.1:-42322308 | MS.gene009229:CDS |
| TTCTACTGAAAAGGAATTAA+AGG | 0.202760 | 8.1:-42316167 | MS.gene009229:intron |
| TCGTATTGAGGAAAGGAAAT+AGG | 0.238108 | 8.1:+42322787 | None:intergenic |
| CTCTTAATATCGCCGCTCTT+TGG | 0.242363 | 8.1:-42322205 | MS.gene009229:CDS |
| TGGTGATGGTGCTGCTAATC+AGG | 0.297061 | 8.1:-42322243 | MS.gene009229:CDS |
| GGTTTCGACGGTTAGGGTTT+CGG | 0.303370 | 8.1:+42322739 | None:intergenic |
| GGATGGAGGTTTCGACGGTT+AGG | 0.337250 | 8.1:+42322732 | None:intergenic |
| AATGTATATGTTAAAGGCTT+AGG | 0.339639 | 8.1:-42314853 | MS.gene009229:CDS |
| TCTTAATATCGCCGCTCTTT+GGG | 0.347641 | 8.1:-42322204 | MS.gene009229:CDS |
| AGCTTTCTTACTCTCTCAAT+TGG | 0.359233 | 8.1:+42316208 | None:intergenic |
| GCAGAAACCGCGGATGAGTT+TGG | 0.369250 | 8.1:+42322580 | None:intergenic |
| ATTTACTTTGTACAGGTAGA+TGG | 0.383832 | 8.1:-42320456 | MS.gene009229:intron |
| ATGTATATGTTAAAGGCTTA+GGG | 0.394667 | 8.1:-42314852 | MS.gene009229:CDS |
| GTTGGAGCTCAGATTCCTCT+TGG | 0.398329 | 8.1:-42322326 | MS.gene009229:CDS |
| GATTCCTCTTGGAGTTGGAT+TGG | 0.401098 | 8.1:-42322315 | MS.gene009229:CDS |
| TGGCCACTCCATGTCTGATC+CGG | 0.401479 | 8.1:-42316369 | MS.gene009229:CDS |
| CTTGTAATACGCAGGACTTT+TGG | 0.405631 | 8.1:+42322028 | None:intergenic |
| TAATGTCCAGATTCTTGAAA+TGG | 0.407066 | 8.1:-42316408 | MS.gene009229:intron |
| TATGGTGGACATGGGATTGT+TGG | 0.408224 | 8.1:-42322344 | MS.gene009229:CDS |
| TGGAAGGTGGTTCGCAGTTG+TGG | 0.409089 | 8.1:+42322699 | None:intergenic |
| ATATGGTTATACCTTTGCTT+TGG | 0.412121 | 8.1:+42315014 | None:intergenic |
| GCTCAGATTCCTCTTGGAGT+TGG | 0.414889 | 8.1:-42322320 | MS.gene009229:CDS |
| CTTTGTACAGGTAGATGGTA+TGG | 0.416779 | 8.1:-42320451 | MS.gene009229:intron |
| CGTATTGAGGAAAGGAAATA+GGG | 0.419858 | 8.1:+42322788 | None:intergenic |
| GATGGAGGTTTCGACGGTTA+GGG | 0.431680 | 8.1:+42322733 | None:intergenic |
| GGTTAGGGTTTCGGTGGAAG+AGG | 0.446559 | 8.1:+42322748 | None:intergenic |
| TGAGTTCGGAGGCGGTGGTT+TGG | 0.454095 | 8.1:+42322669 | None:intergenic |
| GTGGTTTGGACGGTGGTGGA+AGG | 0.454242 | 8.1:+42322683 | None:intergenic |
| AAGCGTGGAGATTATGCTCC+TGG | 0.457396 | 8.1:-42322008 | MS.gene009229:CDS |
| TTCGGAGGCGGTGGTTTGGA+CGG | 0.457880 | 8.1:+42322673 | None:intergenic |
| AGATTATGCTCCTGGATTGA+AGG | 0.459060 | 8.1:-42322000 | MS.gene009229:intron |
| CTGCCATCTCTACGACGGTC+AGG | 0.461075 | 8.1:-42322561 | MS.gene009229:CDS |
| ATCTTTCTAACCTTCAATCC+AGG | 0.464660 | 8.1:+42321990 | None:intergenic |
| TTCGGTGGAAGAGGAAGAAA+TGG | 0.466080 | 8.1:+42322757 | None:intergenic |
| GGAAGGTGGTTCGCAGTTGT+GGG | 0.469466 | 8.1:+42322700 | None:intergenic |
| GGCGGTGGTTTGGACGGTGG+TGG | 0.470193 | 8.1:+42322679 | None:intergenic |
| TACAAAGCAAAAGTAACATT+GGG | 0.474957 | 8.1:+42322266 | None:intergenic |
| CGATTCTCATCCTCTCAATT+CGG | 0.478801 | 8.1:-42322827 | MS.gene009229:CDS |
| CATGGAACCACCTTTCCCCT+TGG | 0.480315 | 8.1:+42322394 | None:intergenic |
| TCACGACATTTCTACTGAAA+AGG | 0.484619 | 8.1:-42316176 | MS.gene009229:CDS |
| GAATTGATGGGAAGGAAAGA+TGG | 0.489679 | 8.1:-42322422 | MS.gene009229:CDS |
| TTATACCTTTGCTTTGGCAA+TGG | 0.489696 | 8.1:+42315020 | None:intergenic |
| GAGAGTAAGAAAGCTGGTAT+TGG | 0.491864 | 8.1:-42316200 | MS.gene009229:CDS |
| CGCAGTTGTGGGAAGTGAAA+GGG | 0.491966 | 8.1:+42322711 | None:intergenic |
| ATTTGTGTAACAGATGGAAT+GGG | 0.492493 | 8.1:-42322071 | MS.gene009229:intron |
| AAGGAACATGCTTTGAAGAA+TGG | 0.493732 | 8.1:-42320393 | MS.gene009229:CDS |
| GGACATGAGTTCGGAGGCGG+TGG | 0.495990 | 8.1:+42322664 | None:intergenic |
| TGAGATTAGCGGTGTTAGAC+AGG | 0.497125 | 8.1:-42316324 | MS.gene009229:intron |
| GATTTGTGTAACAGATGGAA+TGG | 0.503553 | 8.1:-42322072 | MS.gene009229:intron |
| CGCGTGCGGTATGTGCTGCC+CGG | 0.506711 | 8.1:+42316350 | None:intergenic |
| AATGGAACGGTGTCGTATTG+AGG | 0.512155 | 8.1:+42322775 | None:intergenic |
| GAGAACCATGTCGTTGAAGA+AGG | 0.516045 | 8.1:+42322643 | None:intergenic |
| ACATTGGTAAATAGATCAGA+TGG | 0.517521 | 8.1:+42314871 | None:intergenic |
| GGCCACTCCATGTCTGATCC+GGG | 0.519101 | 8.1:-42316368 | MS.gene009229:CDS |
| TTCTCACAAACCAAAATAGC+AGG | 0.519315 | 8.1:+42322176 | None:intergenic |
| GTAAATAGATCAGATGGGTC+TGG | 0.522363 | 8.1:+42314877 | None:intergenic |
| TGCAGGCATGTGGTGCTGAT+AGG | 0.523890 | 8.1:-42314522 | MS.gene009229:intron |
| GTTGAAGAAGGACATGAGTT+CGG | 0.527595 | 8.1:+42322655 | None:intergenic |
| GGTCAGGAAGCCGTTGCTGT+TGG | 0.530115 | 8.1:-42322545 | MS.gene009229:CDS |
| GTTTGGACGGTGGTGGAAGG+TGG | 0.531937 | 8.1:+42322686 | None:intergenic |
| ATACAAAGCAAAAGTAACAT+TGG | 0.535697 | 8.1:+42322265 | None:intergenic |
| CATGGTTCTCATGCGCCGTA+TGG | 0.536252 | 8.1:-42322630 | MS.gene009229:CDS |
| AGGAAAGATGGGTGTTCCAA+GGG | 0.540079 | 8.1:-42322410 | MS.gene009229:CDS |
| GGAAGCCGTTGCTGTTGGAA+TGG | 0.541582 | 8.1:-42322540 | MS.gene009229:CDS |
| TCGCAGTTGTGGGAAGTGAA+AGG | 0.544039 | 8.1:+42322710 | None:intergenic |
| AAGGAAAGATGGGTGTTCCA+AGG | 0.549770 | 8.1:-42322411 | MS.gene009229:CDS |
| GTGGGAAGTGAAAGGGATGG+AGG | 0.555368 | 8.1:+42322718 | None:intergenic |
| TGTTAAAGGCTTAGGGGTCG+AGG | 0.557159 | 8.1:-42314845 | MS.gene009229:intron |
| GGAGGCGGTGGTTTGGACGG+TGG | 0.567848 | 8.1:+42322676 | None:intergenic |
| TAAGCCTTTAACATATACAT+TGG | 0.568328 | 8.1:+42314855 | None:intergenic |
| CTTCCTGACCGTCGTAGAGA+TGG | 0.569109 | 8.1:+42322558 | None:intergenic |
| TGCTTTGAAGAATGGTCCCC+TGG | 0.570115 | 8.1:-42320385 | MS.gene009229:intron |
| AAAGGAAATAGGGTTTGAGA+AGG | 0.572428 | 8.1:+42322798 | None:intergenic |
| GGGTGTTCCAAGGGGAAAGG+TGG | 0.573684 | 8.1:-42322401 | MS.gene009229:CDS |
| CATGTCCTTCTTCAACGACA+TGG | 0.574969 | 8.1:-42322648 | MS.gene009229:CDS |
| GAAGGACATGAGTTCGGAGG+CGG | 0.576411 | 8.1:+42322661 | None:intergenic |
| AAGGAAATAGGGTTTGAGAA+GGG | 0.576534 | 8.1:+42322799 | None:intergenic |
| AGCTTCCATTCCAACAGCAA+CGG | 0.577026 | 8.1:+42322535 | None:intergenic |
| GAAAGGGATGGAGGTTTCGA+CGG | 0.581945 | 8.1:+42322727 | None:intergenic |
| GATCAGACATGGAGTGGCCA+TGG | 0.584534 | 8.1:+42316372 | None:intergenic |
| GAAATAGGGTTTGAGAAGGG+TGG | 0.590452 | 8.1:+42322802 | None:intergenic |
| TTCGACGGTTAGGGTTTCGG+TGG | 0.592878 | 8.1:+42322742 | None:intergenic |
| AATTGATGGGAAGGAAAGAT+GGG | 0.593176 | 8.1:-42322421 | MS.gene009229:CDS |
| TGTGCTGCCCGGATCAGACA+TGG | 0.594191 | 8.1:+42316361 | None:intergenic |
| GATGGGTGTTCCAAGGGGAA+AGG | 0.595669 | 8.1:-42322404 | MS.gene009229:CDS |
| CGAAGCCATTGCCAAAGCAA+AGG | 0.598171 | 8.1:-42315025 | MS.gene009229:intron |
| AATTGAGAGAGTAAGAAAGC+TGG | 0.598823 | 8.1:-42316206 | MS.gene009229:CDS |
| TGCCCGGATCAGACATGGAG+TGG | 0.600111 | 8.1:+42316366 | None:intergenic |
| GCAAGCTTGCAAATTTGCAA+AGG | 0.602106 | 8.1:-42320412 | MS.gene009229:CDS |
| GGTGATGGTGCTGCTAATCA+GGG | 0.617038 | 8.1:-42322242 | MS.gene009229:CDS |
| AGTCAGCAGCGATCTCCATA+CGG | 0.627581 | 8.1:+42322615 | None:intergenic |
| AACGGTGTCGTATTGAGGAA+AGG | 0.627993 | 8.1:+42322780 | None:intergenic |
| AGTACAGTGATCACGATAAG+CGG | 0.629898 | 8.1:+42322484 | None:intergenic |
| CGCACGCGTGATGAGATTAG+CGG | 0.635779 | 8.1:-42316335 | MS.gene009229:CDS |
| CGCTAATCTCATCACGCGTG+CGG | 0.639390 | 8.1:+42316336 | None:intergenic |
| AAGGGTGGATCCGAATTGAG+AGG | 0.642462 | 8.1:+42322817 | None:intergenic |
| ATGGACACTTACAGATACCA+TGG | 0.644817 | 8.1:-42316389 | MS.gene009229:CDS |
| AGTCCTGCGTATTACAAGCG+TGG | 0.644994 | 8.1:-42322023 | MS.gene009229:CDS |
| AAAGCCAATCCAACTCCAAG+AGG | 0.648911 | 8.1:+42322311 | None:intergenic |
| CGTAGAGATGGCAGAAACCG+CGG | 0.660828 | 8.1:+42322570 | None:intergenic |
| CATTGGTAAATAGATCAGAT+GGG | 0.666454 | 8.1:+42314872 | None:intergenic |
| TACAAAGCCAAACTCATCCG+CGG | 0.667143 | 8.1:-42322587 | MS.gene009229:CDS |
| TGTATATGTTAAAGGCTTAG+GGG | 0.667239 | 8.1:-42314851 | MS.gene009229:CDS |
| ATGGAATGGGGACTGCTGAG+TGG | 0.670807 | 8.1:-42322058 | MS.gene009229:CDS |
| TGGAAGAGGAAGAAATGGAA+CGG | 0.671358 | 8.1:+42322762 | None:intergenic |
| TCTCCACGCTTGTAATACGC+AGG | 0.672536 | 8.1:+42322020 | None:intergenic |
| AGCAGGAAGATCCCAAAGAG+CGG | 0.675016 | 8.1:+42322193 | None:intergenic |
| GGAAAGATGGGTGTTCCAAG+GGG | 0.677477 | 8.1:-42322409 | MS.gene009229:CDS |
| GAAGAAGGACATGAGTTCGG+AGG | 0.678757 | 8.1:+42322658 | None:intergenic |
| GTTGTGGGAAGTGAAAGGGA+TGG | 0.697878 | 8.1:+42322715 | None:intergenic |
| GGTTTCTGCCATCTCTACGA+CGG | 0.718050 | 8.1:-42322566 | MS.gene009229:CDS |
| TTTGTGTAACAGATGGAATG+GGG | 0.720617 | 8.1:-42322070 | MS.gene009229:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 42314495 | 42322861 | 42314495 | ID=MS.gene009229 |
| chr8.1 | mRNA | 42314495 | 42322861 | 42314495 | ID=MS.gene009229.t1;Parent=MS.gene009229 |
| chr8.1 | exon | 42322168 | 42322861 | 42322168 | ID=MS.gene009229.t1.exon1;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42322168 | 42322861 | 42322168 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42322001 | 42322080 | 42322001 | ID=MS.gene009229.t1.exon2;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42322001 | 42322080 | 42322001 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42320386 | 42320463 | 42320386 | ID=MS.gene009229.t1.exon3;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42320386 | 42320463 | 42320386 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42316325 | 42316420 | 42316325 | ID=MS.gene009229.t1.exon4;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42316325 | 42316420 | 42316325 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42316168 | 42316239 | 42316168 | ID=MS.gene009229.t1.exon5;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42316168 | 42316239 | 42316168 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42315026 | 42315079 | 42315026 | ID=MS.gene009229.t1.exon6;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42315026 | 42315079 | 42315026 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42314846 | 42314911 | 42314846 | ID=MS.gene009229.t1.exon7;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42314846 | 42314911 | 42314846 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| chr8.1 | exon | 42314495 | 42314539 | 42314495 | ID=MS.gene009229.t1.exon8;Parent=MS.gene009229.t1 |
| chr8.1 | CDS | 42314495 | 42314539 | 42314495 | ID=cds.MS.gene009229.t1;Parent=MS.gene009229.t1 |
| Gene Sequence |
| Protein sequence |
