Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009443.t1 | XP_003598239.1 | 98.9 | 187 | 2 | 0 | 1 | 187 | 1 | 187 | 2.50E-94 | 354.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009443.t1 | P17407 | 55.3 | 188 | 81 | 3 | 3 | 187 | 4 | 191 | 6.6e-51 | 201.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009443.t1 | G7IYJ3 | 98.9 | 187 | 2 | 0 | 1 | 187 | 1 | 187 | 1.8e-94 | 354.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene009443 | MS.gene77725 | PPI |
| MS.gene009443 | MS.gene72493 | PPI |
| MS.gene009443 | MS.gene77727 | PPI |
| MS.gene009443 | MS.gene045669 | PPI |
| MS.gene009443 | MS.gene78072 | PPI |
| MS.gene009443 | MS.gene72495 | PPI |
| MS.gene009443 | MS.gene045671 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009443.t1 | MTR_3g008840 | 98.930 | 187 | 2 | 0 | 1 | 187 | 1 | 187 | 6.47e-137 | 380 |
| MS.gene009443.t1 | MTR_3g008820 | 93.023 | 172 | 12 | 0 | 16 | 187 | 15 | 186 | 4.51e-120 | 337 |
| MS.gene009443.t1 | MTR_5g012000 | 51.099 | 182 | 84 | 2 | 11 | 187 | 30 | 211 | 1.56e-65 | 200 |
| MS.gene009443.t1 | MTR_4g101760 | 47.917 | 192 | 91 | 2 | 5 | 187 | 11 | 202 | 2.27e-60 | 187 |
| MS.gene009443.t1 | MTR_4g035905 | 48.466 | 163 | 82 | 1 | 25 | 187 | 36 | 196 | 2.64e-49 | 158 |
| MS.gene009443.t1 | MTR_4g025550 | 42.079 | 202 | 101 | 6 | 1 | 187 | 1 | 201 | 2.39e-44 | 146 |
| MS.gene009443.t1 | MTR_4g035870 | 44.785 | 163 | 88 | 1 | 25 | 187 | 36 | 196 | 1.77e-43 | 144 |
| MS.gene009443.t1 | MTR_4g035880 | 44.785 | 163 | 88 | 1 | 25 | 187 | 36 | 196 | 1.07e-42 | 142 |
| MS.gene009443.t1 | MTR_4g025590 | 42.690 | 171 | 89 | 4 | 25 | 187 | 3 | 172 | 1.79e-40 | 135 |
| MS.gene009443.t1 | MTR_4g120825 | 38.350 | 206 | 105 | 5 | 1 | 186 | 1 | 204 | 3.65e-40 | 135 |
| MS.gene009443.t1 | MTR_4g025620 | 40.212 | 189 | 104 | 4 | 7 | 187 | 14 | 201 | 4.33e-40 | 135 |
| MS.gene009443.t1 | MTR_4g120830 | 38.350 | 206 | 105 | 5 | 1 | 186 | 1 | 204 | 5.75e-40 | 135 |
| MS.gene009443.t1 | MTR_4g025610 | 40.212 | 189 | 104 | 4 | 7 | 187 | 14 | 201 | 2.28e-39 | 134 |
| MS.gene009443.t1 | MTR_8g104765 | 37.674 | 215 | 103 | 6 | 1 | 186 | 1 | 213 | 1.48e-38 | 132 |
| MS.gene009443.t1 | MTR_3g008760 | 37.017 | 181 | 102 | 3 | 13 | 182 | 45 | 224 | 1.41e-32 | 117 |
| MS.gene009443.t1 | MTR_7g108790 | 33.929 | 168 | 107 | 3 | 18 | 185 | 26 | 189 | 7.89e-31 | 111 |
| MS.gene009443.t1 | MTR_2g043560 | 40.854 | 164 | 81 | 4 | 24 | 182 | 33 | 185 | 1.90e-30 | 110 |
| MS.gene009443.t1 | MTR_4g124855 | 31.351 | 185 | 117 | 4 | 5 | 182 | 14 | 195 | 1.74e-24 | 95.5 |
| MS.gene009443.t1 | MTR_1g088540 | 31.507 | 146 | 96 | 2 | 40 | 185 | 1 | 142 | 2.89e-23 | 90.5 |
| MS.gene009443.t1 | MTR_4g035855 | 30.457 | 197 | 117 | 4 | 5 | 184 | 9 | 202 | 2.78e-21 | 87.0 |
| MS.gene009443.t1 | MTR_8g023310 | 31.646 | 158 | 101 | 3 | 26 | 179 | 68 | 222 | 2.68e-15 | 73.6 |
| MS.gene009443.t1 | MTR_1g008140 | 28.070 | 171 | 110 | 4 | 20 | 180 | 76 | 243 | 1.90e-14 | 70.9 |
| MS.gene009443.t1 | MTR_8g023150 | 31.138 | 167 | 102 | 6 | 21 | 182 | 72 | 230 | 1.57e-13 | 68.2 |
| MS.gene009443.t1 | MTR_7g112580 | 32.558 | 129 | 83 | 3 | 54 | 182 | 69 | 193 | 3.50e-13 | 67.4 |
| MS.gene009443.t1 | MTR_3g091380 | 27.647 | 170 | 103 | 3 | 22 | 180 | 74 | 234 | 7.56e-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009443.t1 | AT5G62350 | 56.471 | 170 | 70 | 2 | 18 | 184 | 31 | 199 | 8.97e-68 | 206 |
| MS.gene009443.t1 | AT3G47380 | 46.939 | 196 | 91 | 4 | 5 | 187 | 7 | 202 | 2.74e-61 | 189 |
| MS.gene009443.t1 | AT4G25260 | 47.264 | 201 | 92 | 2 | 1 | 187 | 1 | 201 | 1.52e-60 | 187 |
| MS.gene009443.t1 | AT1G62770 | 49.485 | 194 | 89 | 4 | 3 | 187 | 6 | 199 | 1.31e-57 | 180 |
| MS.gene009443.t1 | AT4G12390 | 50.898 | 167 | 79 | 1 | 24 | 187 | 38 | 204 | 2.99e-51 | 164 |
| MS.gene009443.t1 | AT5G62360 | 42.331 | 163 | 91 | 2 | 25 | 187 | 43 | 202 | 6.85e-48 | 155 |
| MS.gene009443.t1 | AT5G20740 | 44.444 | 162 | 86 | 2 | 24 | 182 | 32 | 192 | 4.03e-40 | 135 |
| MS.gene009443.t1 | AT5G20740 | 44.444 | 162 | 86 | 2 | 24 | 182 | 66 | 226 | 9.28e-40 | 135 |
| MS.gene009443.t1 | AT1G14890 | 36.702 | 188 | 105 | 3 | 6 | 184 | 33 | 215 | 1.26e-38 | 132 |
| MS.gene009443.t1 | AT4G00080 | 38.693 | 199 | 104 | 8 | 1 | 182 | 1 | 198 | 2.51e-38 | 131 |
| MS.gene009443.t1 | AT1G62760 | 44.643 | 168 | 86 | 3 | 21 | 185 | 143 | 306 | 7.69e-37 | 130 |
| MS.gene009443.t1 | AT2G01610 | 36.702 | 188 | 105 | 3 | 11 | 184 | 30 | 217 | 2.64e-36 | 126 |
| MS.gene009443.t1 | AT4G25250 | 36.420 | 162 | 102 | 1 | 23 | 183 | 35 | 196 | 1.53e-35 | 123 |
| MS.gene009443.t1 | AT1G70720 | 38.342 | 193 | 103 | 5 | 6 | 186 | 8 | 196 | 2.43e-33 | 118 |
| MS.gene009443.t1 | AT1G70720 | 38.342 | 193 | 103 | 5 | 6 | 186 | 20 | 208 | 2.65e-33 | 118 |
| MS.gene009443.t1 | AT5G51520 | 38.415 | 164 | 92 | 3 | 25 | 180 | 37 | 199 | 4.92e-31 | 112 |
| MS.gene009443.t1 | AT1G23205 | 34.536 | 194 | 112 | 4 | 7 | 186 | 10 | 202 | 1.09e-30 | 111 |
| MS.gene009443.t1 | AT3G62820 | 31.016 | 187 | 115 | 6 | 5 | 184 | 11 | 190 | 7.77e-23 | 90.9 |
| MS.gene009443.t1 | AT5G62340 | 32.039 | 206 | 106 | 8 | 5 | 187 | 12 | 206 | 9.58e-22 | 88.2 |
| MS.gene009443.t1 | AT3G14310 | 30.769 | 156 | 106 | 2 | 26 | 180 | 59 | 213 | 1.12e-20 | 89.0 |
| MS.gene009443.t1 | AT1G53830 | 31.288 | 163 | 110 | 2 | 19 | 180 | 55 | 216 | 4.08e-19 | 84.7 |
| MS.gene009443.t1 | AT2G47670 | 28.750 | 160 | 111 | 2 | 25 | 184 | 48 | 204 | 1.66e-17 | 77.0 |
| MS.gene009443.t1 | AT5G53370 | 28.834 | 163 | 109 | 4 | 19 | 180 | 104 | 260 | 2.60e-17 | 79.3 |
| MS.gene009443.t1 | AT5G53370 | 28.834 | 163 | 109 | 4 | 19 | 180 | 68 | 224 | 2.96e-17 | 79.3 |
| MS.gene009443.t1 | AT3G49220 | 30.625 | 160 | 101 | 4 | 22 | 180 | 128 | 278 | 8.99e-17 | 77.4 |
| MS.gene009443.t1 | AT3G49220 | 30.625 | 160 | 101 | 4 | 22 | 180 | 128 | 278 | 1.01e-16 | 77.8 |
| MS.gene009443.t1 | AT3G49220 | 30.625 | 160 | 101 | 4 | 22 | 180 | 83 | 233 | 1.17e-16 | 77.4 |
| MS.gene009443.t1 | AT3G14300 | 26.111 | 180 | 123 | 4 | 13 | 184 | 258 | 435 | 2.57e-12 | 65.1 |
| MS.gene009443.t1 | AT3G14300 | 27.907 | 172 | 114 | 4 | 21 | 186 | 454 | 621 | 5.24e-12 | 63.9 |
Find 32 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATTGATGAGGGTCTTGT+TGG | 0.397480 | 3.2:+3143229 | None:intergenic |
| GGCTAAGTGATAAAGCTGTT+TGG | 0.397613 | 3.2:+3143205 | None:intergenic |
| AACCATTCTTGATCTAATAG+AGG | 0.404018 | 3.2:+3142900 | None:intergenic |
| TTTGGTGGAAAGGCACTTGA+TGG | 0.433230 | 3.2:-3142934 | MS.gene009443:CDS |
| CCAACAAGACCCTCATCAAT+TGG | 0.458410 | 3.2:-3143229 | MS.gene009443:CDS |
| AACAAAACTCAATCCACAAA+AGG | 0.469923 | 3.2:-3143180 | MS.gene009443:CDS |
| TGAAGAGCAGCATATTCTCT+TGG | 0.472801 | 3.2:+3143120 | None:intergenic |
| ACATGATGATTTGATGAAGT+TGG | 0.487396 | 3.2:+3143311 | None:intergenic |
| AAATCACTGATAGTGTTGAT+AGG | 0.490237 | 3.2:-3143083 | MS.gene009443:CDS |
| AGCACTTGTAGTGATGGATT+TGG | 0.492192 | 3.2:-3142952 | MS.gene009443:CDS |
| GAGATTCAACACAAAGTGTT+GGG | 0.500666 | 3.2:+3143274 | None:intergenic |
| AGCCTCTATTAGATCAAGAA+TGG | 0.523764 | 3.2:-3142902 | MS.gene009443:CDS |
| TCAATATGCTACTAAGCACT+AGG | 0.528472 | 3.2:-3142836 | None:intergenic |
| GAGTTAAGAGAAGGAAAACA+AGG | 0.532043 | 3.2:+3143361 | None:intergenic |
| GTGTTGAAGTTGCAATAGAG+AGG | 0.542818 | 3.2:+3143337 | None:intergenic |
| ACAAAGTGCAAAAGTTTCAA+AGG | 0.542895 | 3.2:-3143150 | MS.gene009443:CDS |
| GATGATTTGATGAAGTTGGT+TGG | 0.544420 | 3.2:+3143315 | None:intergenic |
| AGAGATTCAACACAAAGTGT+TGG | 0.548978 | 3.2:+3143273 | None:intergenic |
| GCCAGCTGAAATCCTCACCT+TGG | 0.553087 | 3.2:+3143022 | None:intergenic |
| TAGAGAGGAGAGTTAAGAGA+AGG | 0.561986 | 3.2:+3143352 | None:intergenic |
| CAATATGCTACTAAGCACTA+GGG | 0.562055 | 3.2:-3142835 | None:intergenic |
| TCCAAGGTGAGGATTTCAGC+TGG | 0.570424 | 3.2:-3143023 | MS.gene009443:CDS |
| CTTAAACTTTGCAAGATCCA+AGG | 0.575402 | 3.2:-3143039 | MS.gene009443:CDS |
| GATGAAAGCACTTGTAGTGA+TGG | 0.588649 | 3.2:-3142958 | MS.gene009443:CDS |
| CTGTTTGGACCAATTGATGA+GGG | 0.590554 | 3.2:+3143220 | None:intergenic |
| TATAAGCAATGTTGAGACAT+GGG | 0.604136 | 3.2:-3142998 | MS.gene009443:CDS |
| ACTTGTAGTGATGGATTTGG+TGG | 0.605401 | 3.2:-3142949 | MS.gene009443:CDS |
| AGTTAAGAGAAGGAAAACAA+GGG | 0.622054 | 3.2:+3143362 | None:intergenic |
| ACTTTGCAAGATCCAAGGTG+AGG | 0.631587 | 3.2:-3143034 | MS.gene009443:CDS |
| GCTGTTTGGACCAATTGATG+AGG | 0.633670 | 3.2:+3143219 | None:intergenic |
| TAGTGATGGATTTGGTGGAA+AGG | 0.647596 | 3.2:-3142944 | MS.gene009443:CDS |
| ATATAAGCAATGTTGAGACA+TGG | 0.650361 | 3.2:-3142999 | MS.gene009443:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAAACTCAATCCACAAA+AGG | - | chr3.2:3143035-3143054 | MS.gene009443:CDS | 30.0% | |
| AACCATTCTTGATCTAATAG+AGG | + | chr3.2:3143318-3143337 | None:intergenic | 30.0% | |
| ACAAAGTGCAAAAGTTTCAA+AGG | - | chr3.2:3143065-3143084 | MS.gene009443:CDS | 30.0% | |
| ACATGATGATTTGATGAAGT+TGG | + | chr3.2:3142907-3142926 | None:intergenic | 30.0% | |
| AGTTAAGAGAAGGAAAACAA+GGG | + | chr3.2:3142856-3142875 | None:intergenic | 30.0% | |
| ATATAAGCAATGTTGAGACA+TGG | - | chr3.2:3143216-3143235 | MS.gene009443:CDS | 30.0% | |
| TATAAGCAATGTTGAGACAT+GGG | - | chr3.2:3143217-3143236 | MS.gene009443:CDS | 30.0% | |
| ! | GTTTAAGCTCTTTTAGTGAA+CGG | + | chr3.2:3143163-3143182 | None:intergenic | 30.0% |
| !! | AAATCACTGATAGTGTTGAT+AGG | - | chr3.2:3143132-3143151 | MS.gene009443:CDS | 30.0% |
| !!! | TTGTGGATTGAGTTTTGTTT+AGG | + | chr3.2:3143034-3143053 | None:intergenic | 30.0% |
| AGAGATTCAACACAAAGTGT+TGG | + | chr3.2:3142945-3142964 | None:intergenic | 35.0% | |
| AGCCTCTATTAGATCAAGAA+TGG | - | chr3.2:3143313-3143332 | MS.gene009443:CDS | 35.0% | |
| CTTAAACTTTGCAAGATCCA+AGG | - | chr3.2:3143176-3143195 | MS.gene009443:CDS | 35.0% | |
| GAGTTAAGAGAAGGAAAACA+AGG | + | chr3.2:3142857-3142876 | None:intergenic | 35.0% | |
| ! | CTTTGTTACAAAGCCTTTTG+TGG | + | chr3.2:3143051-3143070 | None:intergenic | 35.0% |
| ! | GAGATTCAACACAAAGTGTT+GGG | + | chr3.2:3142944-3142963 | None:intergenic | 35.0% |
| ! | GATGATTTGATGAAGTTGGT+TGG | + | chr3.2:3142903-3142922 | None:intergenic | 35.0% |
| CTGTTTGGACCAATTGATGA+GGG | + | chr3.2:3142998-3143017 | None:intergenic | 40.0% | |
| GGCTAAGTGATAAAGCTGTT+TGG | + | chr3.2:3143013-3143032 | None:intergenic | 40.0% | |
| GTGTTGAAGTTGCAATAGAG+AGG | + | chr3.2:3142881-3142900 | None:intergenic | 40.0% | |
| TAGAGAGGAGAGTTAAGAGA+AGG | + | chr3.2:3142866-3142885 | None:intergenic | 40.0% | |
| TGAAGAGCAGCATATTCTCT+TGG | + | chr3.2:3143098-3143117 | None:intergenic | 40.0% | |
| !! | ACTTGTAGTGATGGATTTGG+TGG | - | chr3.2:3143266-3143285 | MS.gene009443:CDS | 40.0% |
| !! | AGCACTTGTAGTGATGGATT+TGG | - | chr3.2:3143263-3143282 | MS.gene009443:CDS | 40.0% |
| !! | GATGAAAGCACTTGTAGTGA+TGG | - | chr3.2:3143257-3143276 | MS.gene009443:CDS | 40.0% |
| !! | TAGTGATGGATTTGGTGGAA+AGG | - | chr3.2:3143271-3143290 | MS.gene009443:CDS | 40.0% |
| ACTTTGCAAGATCCAAGGTG+AGG | - | chr3.2:3143181-3143200 | MS.gene009443:CDS | 45.0% | |
| CCAACAAGACCCTCATCAAT+TGG | - | chr3.2:3142986-3143005 | MS.gene009443:CDS | 45.0% | |
| GCTGTTTGGACCAATTGATG+AGG | + | chr3.2:3142999-3143018 | None:intergenic | 45.0% | |
| ! | CCAATTGATGAGGGTCTTGT+TGG | + | chr3.2:3142989-3143008 | None:intergenic | 45.0% |
| !! | TTTGGTGGAAAGGCACTTGA+TGG | - | chr3.2:3143281-3143300 | MS.gene009443:CDS | 45.0% |
| TCCAAGGTGAGGATTTCAGC+TGG | - | chr3.2:3143192-3143211 | MS.gene009443:CDS | 50.0% | |
| GCCAGCTGAAATCCTCACCT+TGG | + | chr3.2:3143196-3143215 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 3142837 | 3143400 | 3142837 | ID=MS.gene009443 |
| chr3.2 | mRNA | 3142837 | 3143400 | 3142837 | ID=MS.gene009443.t1;Parent=MS.gene009443 |
| chr3.2 | exon | 3142837 | 3143400 | 3142837 | ID=MS.gene009443.t1.exon1;Parent=MS.gene009443.t1 |
| chr3.2 | CDS | 3142837 | 3143400 | 3142837 | ID=cds.MS.gene009443.t1;Parent=MS.gene009443.t1 |
| Gene Sequence |
| Protein sequence |
