AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77725


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77725.t1 MTR_4g035905 95.408 196 9 0 1 196 1 196 4.89e-139 386
MS.gene77725.t1 MTR_4g035870 88.776 196 22 0 1 196 1 196 3.30e-127 356
MS.gene77725.t1 MTR_4g035880 87.755 196 24 0 1 196 1 196 4.70e-125 351
MS.gene77725.t1 MTR_3g008840 47.879 165 84 1 34 196 23 187 6.72e-48 155
MS.gene77725.t1 MTR_3g008820 46.667 165 86 1 34 196 22 186 3.26e-47 153
MS.gene77725.t1 MTR_5g012000 42.683 164 91 1 36 196 48 211 4.21e-44 146
MS.gene77725.t1 MTR_4g101760 38.857 175 104 1 25 196 28 202 8.02e-40 135
MS.gene77725.t1 MTR_4g120825 40.000 170 88 4 36 193 35 202 2.20e-36 126
MS.gene77725.t1 MTR_4g120830 39.412 170 89 4 36 193 35 202 6.68e-36 125
MS.gene77725.t1 MTR_8g104765 39.412 170 89 4 36 193 44 211 2.49e-35 124
MS.gene77725.t1 MTR_3g008760 37.107 159 97 2 36 191 66 224 2.13e-31 114
MS.gene77725.t1 MTR_4g035855 35.204 196 116 3 8 193 8 202 6.32e-31 112
MS.gene77725.t1 MTR_7g108790 35.802 162 96 2 37 195 34 190 7.65e-31 112
MS.gene77725.t1 MTR_2g043560 39.394 165 86 4 36 195 34 189 2.85e-30 110
MS.gene77725.t1 MTR_1g088540 39.516 124 73 1 72 195 22 143 7.49e-27 100
MS.gene77725.t1 MTR_4g124855 32.075 212 113 5 1 195 2 199 1.85e-25 98.2
MS.gene77725.t1 MTR_4g025590 35.593 177 91 5 36 196 3 172 2.21e-21 87.0
MS.gene77725.t1 MTR_4g025550 34.314 204 105 7 10 196 10 201 2.68e-21 87.4
MS.gene77725.t1 MTR_4g025610 35.429 175 94 4 36 196 32 201 3.60e-21 87.0
MS.gene77725.t1 MTR_4g025620 32.683 205 106 6 8 196 13 201 1.94e-20 85.1
MS.gene77725.t1 MTR_3g091380 31.868 182 107 4 19 189 59 234 4.20e-19 84.7
MS.gene77725.t1 MTR_1g008140 31.977 172 88 5 37 189 82 243 4.23e-17 79.0
MS.gene77725.t1 MTR_7g112590 28.221 163 105 4 37 195 59 213 6.17e-16 75.5
MS.gene77725.t1 MTR_8g023310 28.205 156 107 3 37 188 68 222 5.03e-15 73.2
MS.gene77725.t1 MTR_7g112580 39.796 98 50 3 99 192 102 194 5.62e-15 72.8
MS.gene77725.t1 MTR_0036s0140 26.087 184 123 4 8 189 49 221 6.01e-14 68.2
MS.gene77725.t1 MTR_8g023150 32.530 166 91 5 37 192 77 231 8.12e-14 69.3
MS.gene77725.t1 MTR_1g083510 27.389 157 110 3 37 193 56 208 2.58e-13 68.2
MS.gene77725.t1 MTR_7g108905 25.654 191 126 3 10 192 18 200 1.46e-12 65.9
MS.gene77725.t1 MTR_1g083470 27.950 161 106 4 37 193 58 212 6.58e-12 63.9
MS.gene77725.t1 MTR_1g083500 27.950 161 106 4 37 193 58 212 6.58e-12 63.9
MS.gene77725.t1 MTR_4g005170 24.719 178 120 4 26 197 52 221 9.75e-12 63.5
MS.gene77725.t1 MTR_8g104580 28.750 160 91 5 40 192 27 170 1.17e-11 63.2
MS.gene77725.t1 MTR_1g083490 26.752 157 105 4 41 193 67 217 2.51e-11 62.4
MS.gene77725.t1 MTR_8g104655 28.125 160 92 5 40 192 35 178 5.20e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77725.t1 AT5G62360 59.259 162 65 1 36 197 43 203 3.52e-64 197
MS.gene77725.t1 AT5G62350 46.667 165 84 1 36 196 38 202 4.15e-44 146
MS.gene77725.t1 AT1G62770 42.365 203 108 4 1 197 1 200 1.63e-43 144
MS.gene77725.t1 AT1G14890 40.000 200 106 5 4 195 24 217 2.04e-41 139
MS.gene77725.t1 AT1G62760 45.882 170 87 2 30 196 141 308 5.12e-41 141
MS.gene77725.t1 AT2G01610 40.838 191 95 4 21 193 27 217 2.96e-40 137
MS.gene77725.t1 AT3G47380 44.848 165 87 1 36 196 38 202 3.12e-40 136
MS.gene77725.t1 AT4G25260 39.378 193 111 2 10 196 9 201 4.53e-40 135
MS.gene77725.t1 AT5G51520 39.286 196 106 5 9 192 8 202 2.49e-38 131
MS.gene77725.t1 AT4G25250 39.506 162 95 2 31 189 32 193 3.27e-38 130
MS.gene77725.t1 AT5G20740 36.316 190 111 3 8 192 9 193 3.10e-37 128
MS.gene77725.t1 AT5G20740 40.373 161 90 2 37 192 68 227 1.19e-36 128
MS.gene77725.t1 AT4G12390 43.976 166 88 1 36 196 39 204 3.40e-34 120
MS.gene77725.t1 AT1G70720 35.789 190 112 3 10 193 9 194 2.44e-32 115
MS.gene77725.t1 AT1G70720 35.789 190 112 3 10 193 21 206 2.99e-32 115
MS.gene77725.t1 AT1G23205 35.838 173 99 3 30 191 27 198 7.29e-29 107
MS.gene77725.t1 AT3G62820 32.620 187 117 5 11 193 9 190 5.14e-24 94.4
MS.gene77725.t1 AT2G47670 36.875 160 96 2 36 193 48 204 9.93e-24 94.0
MS.gene77725.t1 AT5G53370 34.416 154 96 3 37 189 111 260 5.04e-21 90.5
MS.gene77725.t1 AT5G53370 34.416 154 96 3 37 189 75 224 7.37e-21 90.1
MS.gene77725.t1 AT1G53830 31.013 158 107 2 37 192 62 219 2.52e-20 88.6
MS.gene77725.t1 AT4G00080 32.738 168 93 6 36 189 35 196 3.41e-20 84.7
MS.gene77725.t1 AT3G14310 28.994 169 118 2 26 192 48 216 1.81e-19 85.9
MS.gene77725.t1 AT3G49220 31.073 177 102 7 18 189 117 278 1.93e-15 73.9
MS.gene77725.t1 AT3G49220 31.073 177 102 7 18 189 117 278 3.28e-15 73.6
MS.gene77725.t1 AT3G49220 31.073 177 102 7 18 189 72 233 3.72e-15 73.6
MS.gene77725.t1 AT3G05610 28.750 160 104 4 37 193 58 210 5.05e-15 73.2
MS.gene77725.t1 AT5G27870 27.389 157 110 3 37 193 57 209 5.35e-15 73.2
MS.gene77725.t1 AT3G47670 30.435 161 102 4 37 192 113 268 1.02e-13 68.2
MS.gene77725.t1 AT3G14300 27.976 168 107 4 37 193 271 435 4.49e-12 64.7
MS.gene77725.t1 AT3G05620 25.123 203 125 7 9 192 7 201 7.38e-11 60.8

Find 37 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATGCAAGAGTCACGTTT+AGG 0.288631 4.4:-68505209 None:intergenic
CCTCTCTTGGCTTCAGTCCC+TGG 0.289210 4.4:-68505272 None:intergenic
TTCATAGCTGCCGCCTCTCT+TGG 0.353618 4.4:-68505285 None:intergenic
TGAATAATGTGTTTGATTGT+TGG 0.360872 4.4:-68505080 None:intergenic
ACTGTTGTCTGAACATTACC+TGG 0.391345 4.4:-68505483 None:intergenic
TGATGCAAGAGTCACGTTTA+GGG 0.405900 4.4:-68505208 None:intergenic
AAGCACGCGATTTGTTTGAA+TGG 0.408600 4.4:-68505172 None:intergenic
CAATGAGTGATGTACAAACT+TGG 0.417018 4.4:+68505400 MS.gene77725:CDS
TCAATTGACTAGCAATGCTT+TGG 0.428416 4.4:+68505530 MS.gene77725:CDS
GCGATTTGTTTGAATGGAAT+CGG 0.429201 4.4:-68505166 None:intergenic
AATGTGTTTGATTGTTGGTA+TGG 0.433048 4.4:-68505075 None:intergenic
GGTATGGTCTTTCAATTGCT+TGG 0.442357 4.4:-68505059 None:intergenic
TTGGGTCAGTGCCGCTTTGA+CGG 0.448660 4.4:+68505419 MS.gene77725:CDS
AAGAGAGGCGGCAGCTATGA+AGG 0.468721 4.4:+68505287 MS.gene77725:CDS
TCGGTGAAATGAGTCGCCTT+AGG 0.484339 4.4:+68505358 MS.gene77725:CDS
AGCGAGGTGTAGCATAGTCT+TGG 0.500988 4.4:-68505132 None:intergenic
ACTCGAAATTTGAAGTCCTA+AGG 0.502920 4.4:-68505374 None:intergenic
ATCAACTCTCTCGAAAAGCC+AGG 0.512969 4.4:+68505254 MS.gene77725:CDS
GATGAACTACGAAGGTCTAT+CGG 0.538573 4.4:+68505339 MS.gene77725:CDS
GGGACTGAAGCCAAGAGAGG+CGG 0.548500 4.4:+68505275 MS.gene77725:CDS
TTCGAAGAGATTAATGCCCC+AGG 0.583021 4.4:+68505465 MS.gene77725:CDS
AATGAGTGATGTACAAACTT+GGG 0.584779 4.4:+68505401 MS.gene77725:CDS
CTGTTGTCTGAACATTACCT+GGG 0.588535 4.4:-68505482 None:intergenic
AATGTTCAGACAACAGTGAG+AGG 0.607570 4.4:+68505489 MS.gene77725:CDS
TGCATCAGCGAAATCAACCT+CGG 0.612610 4.4:+68505224 MS.gene77725:CDS
CCAGGGACTGAAGCCAAGAG+AGG 0.615497 4.4:+68505272 MS.gene77725:CDS
AGAGTGTGTAATGAAACTAA+AGG 0.619326 4.4:-68505034 None:intergenic
ATCGGCGTGCTTCACTAGCG+AGG 0.620746 4.4:-68505148 None:intergenic
ATTAATCTCTTCGAATCCGT+CGG 0.641331 4.4:-68505457 None:intergenic
GATGAGAGTACTTGTACCGA+CGG 0.647497 4.4:+68505441 MS.gene77725:CDS
TCAACTCTCTCGAAAAGCCA+GGG 0.652947 4.4:+68505255 MS.gene77725:CDS
AGACCTTCGTAGTTCATCAA+CGG 0.656485 4.4:-68505334 None:intergenic
GAGAGTTGATATCACAGCCG+AGG 0.662740 4.4:-68505241 None:intergenic
ATGTTCAGACAACAGTGAGA+GGG 0.673943 4.4:+68505490 MS.gene77725:CDS
AGTACTCTCATCCGTCAAAG+CGG 0.677243 4.4:-68505430 None:intergenic
TGTTGTCTGAACATTACCTG+GGG 0.722489 4.4:-68505481 None:intergenic
ACTCCGTTGATGAACTACGA+AGG 0.736509 4.4:+68505331 MS.gene77725:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGGCAAAAATTATTAGA+AGG - chr4.4:68505020-68505039 None:intergenic 20.0%
! TGAATAATGTGTTTGATTGT+TGG - chr4.4:68505083-68505102 None:intergenic 25.0%
AATGAGTGATGTACAAACTT+GGG + chr4.4:68505401-68505420 MS.gene77725:CDS 30.0%
AATGTGTTTGATTGTTGGTA+TGG - chr4.4:68505078-68505097 None:intergenic 30.0%
AGAGTGTGTAATGAAACTAA+AGG - chr4.4:68505037-68505056 None:intergenic 30.0%
ATTAATCTCTTCGAATCCGT+CGG - chr4.4:68505460-68505479 None:intergenic 35.0%
CAATGAGTGATGTACAAACT+TGG + chr4.4:68505400-68505419 MS.gene77725:CDS 35.0%
TCAATTGACTAGCAATGCTT+TGG + chr4.4:68505530-68505549 MS.gene77725:CDS 35.0%
! ACTCGAAATTTGAAGTCCTA+AGG - chr4.4:68505377-68505396 None:intergenic 35.0%
! GCGATTTGTTTGAATGGAAT+CGG - chr4.4:68505169-68505188 None:intergenic 35.0%
AATGTTCAGACAACAGTGAG+AGG + chr4.4:68505489-68505508 MS.gene77725:CDS 40.0%
ACTGTTGTCTGAACATTACC+TGG - chr4.4:68505486-68505505 None:intergenic 40.0%
AGACCTTCGTAGTTCATCAA+CGG - chr4.4:68505337-68505356 None:intergenic 40.0%
ATGTTCAGACAACAGTGAGA+GGG + chr4.4:68505490-68505509 MS.gene77725:CDS 40.0%
CTGTTGTCTGAACATTACCT+GGG - chr4.4:68505485-68505504 None:intergenic 40.0%
GATGAACTACGAAGGTCTAT+CGG + chr4.4:68505339-68505358 MS.gene77725:CDS 40.0%
GGTATGGTCTTTCAATTGCT+TGG - chr4.4:68505062-68505081 None:intergenic 40.0%
TGATGCAAGAGTCACGTTTA+GGG - chr4.4:68505211-68505230 None:intergenic 40.0%
TGTTGTCTGAACATTACCTG+GGG - chr4.4:68505484-68505503 None:intergenic 40.0%
! AAGCACGCGATTTGTTTGAA+TGG - chr4.4:68505175-68505194 None:intergenic 40.0%
! TCGCGTGCTTTTAACATGTA+CGG + chr4.4:68505185-68505204 MS.gene77725:CDS 40.0%
AGTACTCTCATCCGTCAAAG+CGG - chr4.4:68505433-68505452 None:intergenic 45.0%
ATCAACTCTCTCGAAAAGCC+AGG + chr4.4:68505254-68505273 MS.gene77725:CDS 45.0%
CTGATGCAAGAGTCACGTTT+AGG - chr4.4:68505212-68505231 None:intergenic 45.0%
TCAACTCTCTCGAAAAGCCA+GGG + chr4.4:68505255-68505274 MS.gene77725:CDS 45.0%
TGCATCAGCGAAATCAACCT+CGG + chr4.4:68505224-68505243 MS.gene77725:CDS 45.0%
TTCGAAGAGATTAATGCCCC+AGG + chr4.4:68505465-68505484 MS.gene77725:CDS 45.0%
! ACTCCGTTGATGAACTACGA+AGG + chr4.4:68505331-68505350 MS.gene77725:CDS 45.0%
!! GATGAGAGTACTTGTACCGA+CGG + chr4.4:68505441-68505460 MS.gene77725:CDS 45.0%
AGCGAGGTGTAGCATAGTCT+TGG - chr4.4:68505135-68505154 None:intergenic 50.0%
TCGGTGAAATGAGTCGCCTT+AGG + chr4.4:68505358-68505377 MS.gene77725:CDS 50.0%
!! GAGAGTTGATATCACAGCCG+AGG - chr4.4:68505244-68505263 None:intergenic 50.0%
AAGAGAGGCGGCAGCTATGA+AGG + chr4.4:68505287-68505306 MS.gene77725:CDS 55.0%
TTCATAGCTGCCGCCTCTCT+TGG - chr4.4:68505288-68505307 None:intergenic 55.0%
!! TTGGGTCAGTGCCGCTTTGA+CGG + chr4.4:68505419-68505438 MS.gene77725:CDS 55.0%
ATCGGCGTGCTTCACTAGCG+AGG - chr4.4:68505151-68505170 None:intergenic 60.0%
CCAGGGACTGAAGCCAAGAG+AGG + chr4.4:68505272-68505291 MS.gene77725:CDS 60.0%
CCTCTCTTGGCTTCAGTCCC+TGG - chr4.4:68505275-68505294 None:intergenic 60.0%
GGGACTGAAGCCAAGAGAGG+CGG + chr4.4:68505275-68505294 MS.gene77725:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 68504994 68505587 68504994 ID=MS.gene77725
chr4.4 mRNA 68504994 68505587 68504994 ID=MS.gene77725.t1;Parent=MS.gene77725
chr4.4 exon 68504994 68505587 68504994 ID=MS.gene77725.t1.exon1;Parent=MS.gene77725.t1
chr4.4 CDS 68504994 68505587 68504994 ID=cds.MS.gene77725.t1;Parent=MS.gene77725.t1
Gene Sequence

>MS.gene77725

ATGGAGGCTTATTCTTCAAAGATCCTTCTAATAATTTTTGCCTTTAGTTTCATTACACACTCTATCCAAGCAATTGAAAGACCATACCAACAATCAAACACATTATTCATTAAAAATTCTTGCAGCTCTACAACTTATCCAAGACTATGCTACACCTCGCTAGTGAAGCACGCCGATTCCATTCAAACAAATCGCGTGCTTTTAACATGTACGGCCCTAAACGTGACTCTTGCATCAGCGAAATCAACCTCGGCTGTGATATCAACTCTCTCGAAAAGCCAGGGACTGAAGCCAAGAGAGGCGGCAGCTATGAAGGATTGCGTTGAAGTGCTGAGTGACTCCGTTGATGAACTACGAAGGTCTATCGGTGAAATGAGTCGCCTTAGGACTTCAAATTTCGAGTTAACAATGAGTGATGTACAAACTTGGGTCAGTGCCGCTTTGACGGATGAGAGTACTTGTACCGACGGATTCGAAGAGATTAATGCCCCAGGTAATGTTCAGACAACAGTGAGAGGGAAAATTGTGCAAGTAGCTCAATTGACTAGCAATGCTTTGGCTTTGATAAATAAACTTGCTACTTCACATAGTTAA

Protein sequence

>MS.gene77725.t1

MEAYSSKILLIIFAFSFITHSIQAIERPYQQSNTLFIKNSCSSTTYPRLCYTSLVKHADSIQTNRVLLTCTALNVTLASAKSTSAVISTLSKSQGLKPREAAAMKDCVEVLSDSVDELRRSIGEMSRLRTSNFELTMSDVQTWVSAALTDESTCTDGFEEINAPGNVQTTVRGKIVQVAQLTSNALALINKLATSHS