AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77727


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77727.t1 MTR_4g035870 94.416 197 11 0 1 197 1 197 6.30e-138 383
MS.gene77727.t1 MTR_4g035880 94.416 197 11 0 1 197 1 197 1.01e-137 383
MS.gene77727.t1 MTR_4g035905 83.673 196 32 0 1 196 1 196 9.10e-119 335
MS.gene77727.t1 MTR_3g008820 43.558 163 90 1 36 196 24 186 1.15e-42 142
MS.gene77727.t1 MTR_5g012000 42.683 164 91 1 36 196 48 211 1.65e-42 142
MS.gene77727.t1 MTR_3g008840 43.558 163 90 1 36 196 25 187 1.82e-42 141
MS.gene77727.t1 MTR_4g101760 39.645 169 99 1 31 196 34 202 2.63e-40 136
MS.gene77727.t1 MTR_4g120825 41.566 166 85 3 36 190 35 199 1.08e-36 127
MS.gene77727.t1 MTR_4g120830 40.964 166 86 3 36 190 35 199 3.28e-36 125
MS.gene77727.t1 MTR_8g104765 40.964 166 86 3 36 190 44 208 8.67e-36 125
MS.gene77727.t1 MTR_4g035855 38.509 161 95 2 36 193 43 202 2.01e-31 113
MS.gene77727.t1 MTR_2g043560 39.394 165 86 4 36 195 34 189 1.58e-30 111
MS.gene77727.t1 MTR_7g108790 34.591 159 102 1 37 195 34 190 1.89e-29 108
MS.gene77727.t1 MTR_3g008760 35.443 158 99 2 36 190 66 223 5.18e-29 107
MS.gene77727.t1 MTR_1g088540 39.683 126 70 2 72 195 22 143 3.38e-27 101
MS.gene77727.t1 MTR_4g124855 31.429 210 117 4 1 195 2 199 3.46e-25 97.4
MS.gene77727.t1 MTR_4g025590 33.333 171 103 3 34 194 1 170 1.12e-19 82.4
MS.gene77727.t1 MTR_1g008140 33.333 180 91 5 32 189 71 243 2.33e-19 85.5
MS.gene77727.t1 MTR_4g025550 33.728 169 101 3 36 194 32 199 3.72e-19 82.0
MS.gene77727.t1 MTR_4g025610 33.136 169 102 3 36 194 32 199 4.14e-19 81.6
MS.gene77727.t1 MTR_3g091380 35.849 159 94 3 37 189 78 234 4.20e-19 84.7
MS.gene77727.t1 MTR_4g025620 33.136 169 102 3 36 194 32 199 3.35e-18 79.3
MS.gene77727.t1 MTR_8g023310 26.875 160 114 2 37 193 68 227 1.89e-17 80.1
MS.gene77727.t1 MTR_7g112590 29.630 162 104 5 37 195 59 213 2.86e-17 79.3
MS.gene77727.t1 MTR_1g083470 28.108 185 118 6 15 193 37 212 5.68e-15 72.8
MS.gene77727.t1 MTR_1g083500 28.108 185 118 6 15 193 37 212 5.68e-15 72.8
MS.gene77727.t1 MTR_7g112580 38.235 102 54 3 96 193 99 195 1.67e-14 71.6
MS.gene77727.t1 MTR_0036s0140 29.444 180 110 5 13 189 56 221 4.47e-14 68.6
MS.gene77727.t1 MTR_4g005170 25.989 177 117 5 25 195 51 219 1.33e-13 68.9
MS.gene77727.t1 MTR_1g083510 27.160 162 104 5 37 193 56 208 1.35e-13 68.9
MS.gene77727.t1 MTR_4g120290 31.613 155 96 4 41 193 63 209 5.89e-13 67.0
MS.gene77727.t1 MTR_8g023150 31.288 163 89 5 41 192 81 231 8.53e-13 66.6
MS.gene77727.t1 MTR_1g083490 26.286 175 115 5 25 193 51 217 1.83e-12 65.5
MS.gene77727.t1 MTR_4g120280 30.323 155 98 4 41 193 63 209 2.16e-11 62.4
MS.gene77727.t1 MTR_4g124550 32.632 95 60 1 100 194 143 233 2.91e-11 62.0
MS.gene77727.t1 MTR_8g104580 28.235 170 96 6 34 193 18 171 6.87e-11 60.8
MS.gene77727.t1 MTR_4g005180 25.714 175 116 5 25 193 33 199 7.35e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77727.t1 AT5G62360 56.790 162 69 1 36 197 43 203 2.08e-62 192
MS.gene77727.t1 AT1G62760 46.746 169 85 2 31 196 142 308 3.35e-43 147
MS.gene77727.t1 AT5G62350 45.679 162 84 1 36 193 38 199 1.15e-41 139
MS.gene77727.t1 AT1G62770 45.732 164 83 3 36 193 33 196 3.03e-40 136
MS.gene77727.t1 AT1G14890 41.053 190 99 4 9 190 28 212 2.16e-38 132
MS.gene77727.t1 AT2G01610 41.714 175 84 3 33 190 41 214 2.63e-38 132
MS.gene77727.t1 AT3G47380 44.444 162 86 1 36 193 38 199 5.59e-38 130
MS.gene77727.t1 AT4G25260 40.000 165 95 1 36 196 37 201 1.06e-36 127
MS.gene77727.t1 AT5G51520 36.735 196 111 4 9 192 8 202 3.86e-35 123
MS.gene77727.t1 AT5G20740 35.385 195 118 3 8 197 7 198 3.88e-35 123
MS.gene77727.t1 AT5G20740 38.323 167 97 2 36 197 67 232 1.23e-34 122
MS.gene77727.t1 AT4G25250 36.420 162 100 2 31 189 32 193 4.84e-34 120
MS.gene77727.t1 AT1G70720 36.649 191 111 3 7 191 6 192 1.48e-30 111
MS.gene77727.t1 AT1G70720 36.649 191 111 3 7 191 18 204 1.84e-30 111
MS.gene77727.t1 AT1G23205 37.725 167 92 3 36 191 33 198 1.31e-29 108
MS.gene77727.t1 AT4G12390 41.718 163 90 1 36 193 39 201 5.60e-29 107
MS.gene77727.t1 AT2G47670 34.810 158 102 1 36 193 48 204 7.75e-23 91.7
MS.gene77727.t1 AT3G14310 30.460 174 118 3 23 193 44 217 8.73e-23 95.5
MS.gene77727.t1 AT3G62820 34.161 161 100 4 36 193 33 190 2.23e-22 89.7
MS.gene77727.t1 AT1G53830 29.560 159 110 2 37 193 62 220 3.07e-21 90.9
MS.gene77727.t1 AT5G53370 34.416 154 96 3 37 189 111 260 3.75e-20 87.8
MS.gene77727.t1 AT5G53370 34.416 154 96 3 37 189 75 224 5.66e-20 87.4
MS.gene77727.t1 AT4G00080 31.098 164 101 5 36 189 35 196 1.85e-18 80.1
MS.gene77727.t1 AT3G05610 29.341 167 102 6 37 197 58 214 2.54e-15 73.9
MS.gene77727.t1 AT5G27870 26.994 163 111 4 37 197 57 213 7.76e-15 72.4
MS.gene77727.t1 AT3G14300 27.014 211 136 5 3 196 229 438 2.98e-14 70.9
MS.gene77727.t1 AT3G49220 33.121 157 91 5 37 189 132 278 3.73e-14 70.5
MS.gene77727.t1 AT3G49220 33.121 157 91 5 37 189 87 233 4.84e-14 70.1
MS.gene77727.t1 AT3G49220 33.121 157 91 5 37 189 132 278 5.32e-14 70.1
MS.gene77727.t1 AT3G47670 31.088 193 114 9 6 192 89 268 1.51e-12 65.1
MS.gene77727.t1 AT2G26450 28.302 159 105 3 37 193 109 260 2.76e-12 65.1
MS.gene77727.t1 AT5G49180 26.875 160 109 4 41 197 59 213 3.77e-12 64.7
MS.gene77727.t1 AT5G51490 27.545 167 104 5 38 195 32 190 1.52e-11 62.8
MS.gene77727.t1 AT1G53840 28.659 164 100 5 41 192 77 235 2.95e-11 62.0

Find 40 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGATTTGTTTGTATGAAAT+TGG 0.232980 4.4:-68606108 None:intergenic
TTCATAGCTGCAGCCTCTCT+TGG 0.298648 4.4:-68606227 None:intergenic
TGAATAATAAGTTGGATTGT+TGG 0.368191 4.4:-68606022 None:intergenic
ATTTCTAATGAATAATAAGT+TGG 0.390843 4.4:-68606030 None:intergenic
AGGGATGTGTAGCATAATCT+TGG 0.405480 4.4:-68606074 None:intergenic
GTCAACTCTCTCAAAAGGTC+AGG 0.407740 4.4:+68606196 MS.gene77727:CDS
CAATGAGTGATGTACAAACT+TGG 0.414535 4.4:+68606342 MS.gene77727:CDS
TCATAGCTGCAGCCTCTCTT+GGG 0.426216 4.4:-68606226 None:intergenic
TTGGGTTAGTGCTGCTTTGA+CGG 0.427334 4.4:+68606361 MS.gene77727:CDS
GGTATGGTCTTTCATTTGCT+TGG 0.429513 4.4:-68606001 None:intergenic
AATAAGTTGGATTGTTGGTA+TGG 0.440873 4.4:-68606017 None:intergenic
TAGAAGGATCCTTGAAGAAA+AGG 0.444342 4.4:-68605943 None:intergenic
AAGAGAGGCTGCAGCTATGA+AGG 0.471251 4.4:+68606229 MS.gene77727:CDS
TTGATGTCAACTCTCTCAAA+AGG 0.498369 4.4:+68606191 MS.gene77727:CDS
TGAAATTGGCATGCTTCACT+AGG 0.501560 4.4:-68606094 None:intergenic
CAAAATTTGATGTGCTAAGA+CGG 0.506965 4.4:-68606313 None:intergenic
GGGATGTGTAGCATAATCTT+GGG 0.510147 4.4:-68606073 None:intergenic
TCATTTCATCGATAGACCTT+CGG 0.524168 4.4:-68606289 None:intergenic
CGATGCAAGAGTCACGTTGA+GGG 0.540531 4.4:-68606150 None:intergenic
ATGTTAAGACAGTGGTGAGA+GGG 0.549785 4.4:+68606432 MS.gene77727:CDS
AATGTTAAGACAGTGGTGAG+AGG 0.559999 4.4:+68606431 MS.gene77727:CDS
ATTTCAAGAGATTAGTGCTA+CGG 0.561546 4.4:+68606406 MS.gene77727:CDS
TCAACTCTCTCAAAAGGTCA+GGG 0.566267 4.4:+68606197 MS.gene77727:CDS
GCATAATCTTGGGTAAGTTG+TGG 0.573599 4.4:-68606063 None:intergenic
AATGAGTGATGTACAAACTT+GGG 0.584779 4.4:+68606343 MS.gene77727:CDS
AATAAACTTTCCACTTCACA+AGG 0.605086 4.4:+68606503 MS.gene77727:CDS
TGCATCGGCAAAATCAACCT+CGG 0.611496 4.4:+68606166 MS.gene77727:CDS
ACTAATCTCTTGAAATCCGT+CGG 0.615355 4.4:-68606399 None:intergenic
CCGATGCAAGAGTCACGTTG+AGG 0.618537 4.4:-68606151 None:intergenic
GATCAAAACACTTGTACCGA+CGG 0.623780 4.4:+68606383 MS.gene77727:CDS
GAAATTGGCATGCTTCACTA+GGG 0.624258 4.4:-68606093 None:intergenic
GAGAGTTGACATCAAAGCCG+AGG 0.641012 4.4:-68606183 None:intergenic
ATTCCGTCGATGAACTCCGA+AGG 0.643719 4.4:+68606273 MS.gene77727:CDS
CCTCAACGTGACTCTTGCAT+CGG 0.643902 4.4:+68606151 MS.gene77727:CDS
CAACTCTCTCAAAAGGTCAG+GGG 0.648709 4.4:+68606198 MS.gene77727:CDS
TCAGGGGATGAACCCAAGAG+AGG 0.650942 4.4:+68606214 MS.gene77727:CDS
AGAGTGTGTGATGAAACTAA+AGG 0.661077 4.4:-68605976 None:intergenic
AGACCTTCGGAGTTCATCGA+CGG 0.662948 4.4:-68606276 None:intergenic
AGCTATGAAGGATTGCGTCG+AGG 0.696363 4.4:+68606241 MS.gene77727:CDS
TACGGAGAATGTTAAGACAG+TGG 0.702949 4.4:+68606424 MS.gene77727:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCTAATGAATAATAAGT+TGG - chr4.4:68606033-68606052 None:intergenic 15.0%
! TAAAGGCAAAAATTGTTAGA+AGG - chr4.4:68605962-68605981 None:intergenic 25.0%
!! TGAATAATAAGTTGGATTGT+TGG - chr4.4:68606025-68606044 None:intergenic 25.0%
AATAAACTTTCCACTTCACA+AGG + chr4.4:68606503-68606522 MS.gene77727:CDS 30.0%
AATGAGTGATGTACAAACTT+GGG + chr4.4:68606343-68606362 MS.gene77727:CDS 30.0%
ATTTCAAGAGATTAGTGCTA+CGG + chr4.4:68606406-68606425 MS.gene77727:CDS 30.0%
CAAAATTTGATGTGCTAAGA+CGG - chr4.4:68606316-68606335 None:intergenic 30.0%
! AATAAGTTGGATTGTTGGTA+TGG - chr4.4:68606020-68606039 None:intergenic 30.0%
! GCGATTTGTTTGTATGAAAT+TGG - chr4.4:68606111-68606130 None:intergenic 30.0%
ACTAATCTCTTGAAATCCGT+CGG - chr4.4:68606402-68606421 None:intergenic 35.0%
AGAGTGTGTGATGAAACTAA+AGG - chr4.4:68605979-68605998 None:intergenic 35.0%
CAATGAGTGATGTACAAACT+TGG + chr4.4:68606342-68606361 MS.gene77727:CDS 35.0%
TAGAAGGATCCTTGAAGAAA+AGG - chr4.4:68605946-68605965 None:intergenic 35.0%
TCATTTCATCGATAGACCTT+CGG - chr4.4:68606292-68606311 None:intergenic 35.0%
TTGATGTCAACTCTCTCAAA+AGG + chr4.4:68606191-68606210 MS.gene77727:CDS 35.0%
! ACAAATCGCATGCTTTTAAC+AGG + chr4.4:68606122-68606141 MS.gene77727:CDS 35.0%
AATGTTAAGACAGTGGTGAG+AGG + chr4.4:68606431-68606450 MS.gene77727:CDS 40.0%
AGGGATGTGTAGCATAATCT+TGG - chr4.4:68606077-68606096 None:intergenic 40.0%
ATGTTAAGACAGTGGTGAGA+GGG + chr4.4:68606432-68606451 MS.gene77727:CDS 40.0%
GAAATTGGCATGCTTCACTA+GGG - chr4.4:68606096-68606115 None:intergenic 40.0%
GATCAAAACACTTGTACCGA+CGG + chr4.4:68606383-68606402 MS.gene77727:CDS 40.0%
GCATAATCTTGGGTAAGTTG+TGG - chr4.4:68606066-68606085 None:intergenic 40.0%
GGGATGTGTAGCATAATCTT+GGG - chr4.4:68606076-68606095 None:intergenic 40.0%
GGTATGGTCTTTCATTTGCT+TGG - chr4.4:68606004-68606023 None:intergenic 40.0%
TACGGAGAATGTTAAGACAG+TGG + chr4.4:68606424-68606443 MS.gene77727:CDS 40.0%
TCAACTCTCTCAAAAGGTCA+GGG + chr4.4:68606197-68606216 MS.gene77727:CDS 40.0%
TGAAATTGGCATGCTTCACT+AGG - chr4.4:68606097-68606116 None:intergenic 40.0%
! TCGCATGCTTTTAACAGGAA+CGG + chr4.4:68606127-68606146 MS.gene77727:CDS 40.0%
CAACTCTCTCAAAAGGTCAG+GGG + chr4.4:68606198-68606217 MS.gene77727:CDS 45.0%
GTCAACTCTCTCAAAAGGTC+AGG + chr4.4:68606196-68606215 MS.gene77727:CDS 45.0%
! TGCATCGGCAAAATCAACCT+CGG + chr4.4:68606166-68606185 MS.gene77727:CDS 45.0%
!! TTGGGTTAGTGCTGCTTTGA+CGG + chr4.4:68606361-68606380 MS.gene77727:CDS 45.0%
AAGAGAGGCTGCAGCTATGA+AGG + chr4.4:68606229-68606248 MS.gene77727:CDS 50.0%
AGACCTTCGGAGTTCATCGA+CGG - chr4.4:68606279-68606298 None:intergenic 50.0%
AGCTATGAAGGATTGCGTCG+AGG + chr4.4:68606241-68606260 MS.gene77727:CDS 50.0%
ATTCCGTCGATGAACTCCGA+AGG + chr4.4:68606273-68606292 MS.gene77727:CDS 50.0%
CCTCAACGTGACTCTTGCAT+CGG + chr4.4:68606151-68606170 MS.gene77727:CDS 50.0%
CGATGCAAGAGTCACGTTGA+GGG - chr4.4:68606153-68606172 None:intergenic 50.0%
GAGAGTTGACATCAAAGCCG+AGG - chr4.4:68606186-68606205 None:intergenic 50.0%
TCATAGCTGCAGCCTCTCTT+GGG - chr4.4:68606229-68606248 None:intergenic 50.0%
TTCATAGCTGCAGCCTCTCT+TGG - chr4.4:68606230-68606249 None:intergenic 50.0%
CCGATGCAAGAGTCACGTTG+AGG - chr4.4:68606154-68606173 None:intergenic 55.0%
TCAGGGGATGAACCCAAGAG+AGG + chr4.4:68606214-68606233 MS.gene77727:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 68605936 68606529 68605936 ID=MS.gene77727
chr4.4 mRNA 68605936 68606529 68605936 ID=MS.gene77727.t1;Parent=MS.gene77727
chr4.4 exon 68605936 68606529 68605936 ID=MS.gene77727.t1.exon1;Parent=MS.gene77727.t1
chr4.4 CDS 68605936 68606529 68605936 ID=cds.MS.gene77727.t1;Parent=MS.gene77727.t1
Gene Sequence

>MS.gene77727

ATGGAGGCCTTTTCTTCAAGGATCCTTCTAACAATTTTTGCCTTTAGTTTCATCACACACTCTATCCAAGCAAATGAAAGACCATACCAACAATCCAACTTATTATTCATTAGAAATTCTTGTAGTTCCACAACTTACCCAAGATTATGCTACACATCCCTAGTGAAGCATGCCAATTTCATACAAACAAATCGCATGCTTTTAACAGGAACGGCCCTCAACGTGACTCTTGCATCGGCAAAATCAACCTCGGCTTTGATGTCAACTCTCTCAAAAGGTCAGGGGATGAACCCAAGAGAGGCTGCAGCTATGAAGGATTGCGTCGAGGTGCTAAGTGATTCCGTCGATGAACTCCGAAGGTCTATCGATGAAATGAGCCGTCTTAGCACATCAAATTTTGAACTAACAATGAGTGATGTACAAACTTGGGTTAGTGCTGCTTTGACGGATCAAAACACTTGTACCGACGGATTTCAAGAGATTAGTGCTACGGAGAATGTTAAGACAGTGGTGAGAGGGAGCATTGTGCAAGTTGCTCAATTGACTAGCAATGCTTTAGCATTGATAAATAAACTTTCCACTTCACAAGGTTAA

Protein sequence

>MS.gene77727.t1

MEAFSSRILLTIFAFSFITHSIQANERPYQQSNLLFIRNSCSSTTYPRLCYTSLVKHANFIQTNRMLLTGTALNVTLASAKSTSALMSTLSKGQGMNPREAAAMKDCVEVLSDSVDELRRSIDEMSRLSTSNFELTMSDVQTWVSAALTDQNTCTDGFQEISATENVKTVVRGSIVQVAQLTSNALALINKLSTSQG