AlfalfaGEDB | MS.gene72493

M. sativa cultivar XinJiangDaYe / MS.gene72493

KEGG: -
GO: GO:0003674 GO:0004857 GO:0030234 GO:0098772
PFAM: PF04043.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72493.t1	XP_013455330.1	94.7	132	7	0	1	132	66	197	4.00E-46	194.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72493.t1	Q9SI74	43.8	128	67	2	7	131	183	308	6.8e-18	91.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72493.t1	A0A072UIY9	94.7	132	7	0	1	132	66	197	2.9e-46	194.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051244	MS.gene72493	0.801423	9.07E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene72493	MS.gene84370	PPI
MS.gene72493	MS.gene009441	PPI
MS.gene72493	MS.gene51614	PPI
MS.gene009443	MS.gene72493	PPI
MS.gene72493	MS.gene009442	PPI
MS.gene72493	MS.gene56244	PPI
MS.gene72493	MS.gene56243	PPI
MS.gene72493	MS.gene84366	PPI
MS.gene72493	MS.gene56242	PPI
MS.gene51615	MS.gene72493	PPI
MS.gene72493	MS.gene84367	PPI
MS.gene84369	MS.gene72493	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72493.t1	MTR_4g035870	95.455	132	6	0	1	132	66	197	1.00e-87	253
MS.gene72493.t1	MTR_4g035880	93.182	132	9	0	1	132	66	197	6.03e-86	249
MS.gene72493.t1	MTR_4g035905	83.206	131	22	0	1	131	66	196	7.64e-74	218
MS.gene72493.t1	MTR_5g012000	43.939	132	71	1	3	131	80	211	7.59e-35	120
MS.gene72493.t1	MTR_3g008820	43.511	131	72	1	3	131	56	186	2.86e-33	115
MS.gene72493.t1	MTR_3g008840	43.511	131	72	1	3	131	57	187	1.01e-32	114
MS.gene72493.t1	MTR_4g101760	40.625	128	73	1	7	131	75	202	4.51e-30	107
MS.gene72493.t1	MTR_1g088540	40.476	126	69	2	7	130	22	143	2.65e-28	101
MS.gene72493.t1	MTR_2g043560	44.961	129	58	3	7	130	69	189	2.04e-26	98.2
MS.gene72493.t1	MTR_7g108790	37.097	124	76	1	7	130	69	190	1.62e-25	95.9
MS.gene72493.t1	MTR_4g120825	38.806	134	70	3	3	125	67	199	4.56e-23	89.7
MS.gene72493.t1	MTR_4g120830	38.060	134	71	3	3	125	67	199	1.09e-22	88.6
MS.gene72493.t1	MTR_8g104765	38.060	134	71	3	3	125	76	208	3.60e-22	87.4
MS.gene72493.t1	MTR_4g035855	37.405	131	78	2	1	128	73	202	2.55e-19	79.7
MS.gene72493.t1	MTR_4g124855	36.364	110	70	0	21	130	90	199	2.78e-18	77.4
MS.gene72493.t1	MTR_3g008760	33.858	127	79	3	3	125	98	223	4.18e-16	72.0
MS.gene72493.t1	MTR_1g008140	38.095	105	51	2	32	124	141	243	3.11e-15	71.2
MS.gene72493.t1	MTR_7g112580	37.255	102	55	3	31	128	99	195	1.36e-14	69.3
MS.gene72493.t1	MTR_4g025590	34.307	137	79	3	3	129	35	170	1.48e-14	67.0
MS.gene72493.t1	MTR_3g091380	37.500	128	72	3	3	124	109	234	1.93e-14	68.9
MS.gene72493.t1	MTR_4g025550	34.532	139	76	4	3	129	64	199	3.08e-14	66.6
MS.gene72493.t1	MTR_4g025610	33.577	137	80	3	3	129	64	199	3.35e-14	66.6
MS.gene72493.t1	MTR_4g025620	37.500	104	58	2	32	129	97	199	5.17e-14	66.2
MS.gene72493.t1	MTR_7g112590	29.771	131	83	4	3	130	89	213	1.34e-12	63.5
MS.gene72493.t1	MTR_1g083470	29.703	101	65	2	32	130	118	214	2.32e-12	63.2
MS.gene72493.t1	MTR_1g083500	29.703	101	65	2	32	130	118	214	2.32e-12	63.2
MS.gene72493.t1	MTR_8g023310	31.092	119	79	2	7	123	105	222	4.32e-12	62.4
MS.gene72493.t1	MTR_4g120290	34.259	108	65	2	23	128	106	209	1.75e-11	60.5
MS.gene72493.t1	MTR_2g044660	30.851	94	61	1	35	128	141	230	4.24e-11	59.3
MS.gene72493.t1	MTR_4g124550	31.579	95	61	1	35	129	143	233	5.00e-11	59.3
MS.gene72493.t1	MTR_0036s0140	34.483	116	65	2	12	124	114	221	8.47e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72493.t1	AT5G62360	57.252	131	55	1	2	132	74	203	2.08e-47	152
MS.gene72493.t1	AT1G62770	47.328	131	64	2	3	128	66	196	2.37e-34	118
MS.gene72493.t1	AT1G62760	49.219	128	60	2	7	131	183	308	2.62e-32	116
MS.gene72493.t1	AT5G62350	44.615	130	68	1	3	128	70	199	1.45e-30	108
MS.gene72493.t1	AT4G25260	40.602	133	75	1	3	131	69	201	1.22e-27	101
MS.gene72493.t1	AT3G47380	43.846	130	69	1	3	128	70	199	6.24e-27	99.8
MS.gene72493.t1	AT5G20740	40.000	135	75	2	3	132	65	198	8.76e-27	99.4
MS.gene72493.t1	AT5G20740	40.000	135	75	2	3	132	99	232	2.21e-26	99.0
MS.gene72493.t1	AT2G01610	41.912	136	61	3	7	125	80	214	2.88e-26	98.6
MS.gene72493.t1	AT1G14890	42.188	128	64	3	3	125	90	212	6.40e-23	89.7
MS.gene72493.t1	AT4G12390	41.791	134	73	1	3	131	71	204	2.06e-22	88.2
MS.gene72493.t1	AT4G25250	37.600	125	75	2	3	124	69	193	4.41e-22	87.0
MS.gene72493.t1	AT1G23205	37.500	136	73	3	2	126	64	198	6.98e-22	86.7
MS.gene72493.t1	AT5G51520	39.706	136	69	4	3	127	69	202	7.77e-22	86.7
MS.gene72493.t1	AT1G70720	42.000	100	53	1	34	128	107	206	9.36e-22	86.7
MS.gene72493.t1	AT1G70720	42.000	100	53	1	34	128	95	194	9.84e-22	86.3
MS.gene72493.t1	AT3G62820	43.299	97	52	2	34	128	95	190	3.24e-19	79.3
MS.gene72493.t1	AT2G47670	42.553	94	54	0	35	128	111	204	5.18e-17	73.9
MS.gene72493.t1	AT1G53830	31.452	124	83	2	7	128	97	220	2.21e-16	74.7
MS.gene72493.t1	AT3G14310	32.381	105	70	1	25	128	113	217	5.15e-16	73.6
MS.gene72493.t1	AT5G53370	36.585	123	73	3	3	124	142	260	1.35e-15	72.4
MS.gene72493.t1	AT5G53370	36.585	123	73	3	3	124	106	224	1.61e-15	72.0
MS.gene72493.t1	AT4G00080	33.077	130	78	3	3	124	68	196	2.47e-14	67.0
MS.gene72493.t1	AT5G27870	28.788	132	87	3	3	132	87	213	4.38e-14	68.2
MS.gene72493.t1	AT3G05610	31.884	138	79	5	1	132	86	214	1.78e-13	66.2
MS.gene72493.t1	AT3G49220	37.234	94	53	2	31	124	191	278	3.94e-11	59.3
MS.gene72493.t1	AT3G49220	37.234	94	53	2	31	124	146	233	4.12e-11	59.7

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATAGCTGCAGCCTCTCT+TGG	0.298648	4.2:-69273957	None:intergenic
CCGAAGGTCTATCGATGAAA+TGG	0.365879	4.2:+69274019	MS.gene72493:CDS
GTCAACTCTCTCAAAAGGTC+AGG	0.401169	4.2:+69273926	MS.gene72493:CDS
CAATGAGTGATGTACAAACT+TGG	0.414535	4.2:+69274072	MS.gene72493:CDS
TCATAGCTGCAGCCTCTCTT+GGG	0.426216	4.2:-69273956	None:intergenic
TTGGGTTAGTGCTGCTTTGA+CGG	0.427334	4.2:+69274091	MS.gene72493:CDS
AATGTTAAGACAGTGGTAAG+AGG	0.466616	4.2:+69274161	MS.gene72493:CDS
AAGAGAGGCTGCAGCTATGA+AGG	0.471251	4.2:+69273959	MS.gene72493:CDS
TTGATGTCAACTCTCTCAAA+AGG	0.498369	4.2:+69273921	MS.gene72493:CDS
CGAAGGTCTATCGATGAAAT+GGG	0.502061	4.2:+69274020	MS.gene72493:CDS
CCATTTCATCGATAGACCTT+CGG	0.513898	4.2:-69274019	None:intergenic
CGATGCAAGAGTCACGTTGA+GGG	0.540531	4.2:-69273880	None:intergenic
ATGTTAAGACAGTGGTAAGA+GGG	0.554026	4.2:+69274162	MS.gene72493:CDS
TCAACTCTCTCAAAAGGTCA+GGG	0.559696	4.2:+69273927	MS.gene72493:CDS
ATTTCAAGAGATTAGTGCTA+CGG	0.561546	4.2:+69274136	MS.gene72493:CDS
AATGAGTGATGTACAAACTT+GGG	0.584779	4.2:+69274073	MS.gene72493:CDS
CAAAATTTGATGTACTAAGA+CGG	0.589135	4.2:-69274043	None:intergenic
AATAAACTTTCCACTTCACA+AGG	0.605086	4.2:+69274233	MS.gene72493:CDS
TGCATCGGCAAAATCAACCT+CGG	0.611496	4.2:+69273896	MS.gene72493:CDS
ACTAATCTCTTGAAATCCGT+CGG	0.615355	4.2:-69274129	None:intergenic
CCGATGCAAGAGTCACGTTG+AGG	0.618537	4.2:-69273881	None:intergenic
GATCAAAACACTTGTACCGA+CGG	0.623780	4.2:+69274113	MS.gene72493:CDS
GAGAGTTGACATCAAAGCCG+AGG	0.641012	4.2:-69273913	None:intergenic
ATTCCGTCGATGAACTCCGA+AGG	0.643719	4.2:+69274003	MS.gene72493:CDS
CCTCAACGTGACTCTTGCAT+CGG	0.643902	4.2:+69273881	MS.gene72493:CDS
CAACTCTCTCAAAAGGTCAG+GGG	0.654007	4.2:+69273928	MS.gene72493:CDS
TCAGGGGTTGAACCCAAGAG+AGG	0.654231	4.2:+69273944	MS.gene72493:CDS
AGACCTTCGGAGTTCATCGA+CGG	0.662948	4.2:-69274006	None:intergenic
TACGGAGAATGTTAAGACAG+TGG	0.694614	4.2:+69274154	MS.gene72493:CDS
AGCTATGAAGGATTGCGTCG+AGG	0.696363	4.2:+69273971	MS.gene72493:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAAAATTTGATGTACTAAGA+CGG	-	chr4.2:69274046-69274065	None:intergenic	25.0%
	AATAAACTTTCCACTTCACA+AGG	+	chr4.2:69274233-69274252	MS.gene72493:CDS	30.0%
	AATGAGTGATGTACAAACTT+GGG	+	chr4.2:69274073-69274092	MS.gene72493:CDS	30.0%
	ATTTCAAGAGATTAGTGCTA+CGG	+	chr4.2:69274136-69274155	MS.gene72493:CDS	30.0%
	AATGTTAAGACAGTGGTAAG+AGG	+	chr4.2:69274161-69274180	MS.gene72493:CDS	35.0%
	ACTAATCTCTTGAAATCCGT+CGG	-	chr4.2:69274132-69274151	None:intergenic	35.0%
	ATGTTAAGACAGTGGTAAGA+GGG	+	chr4.2:69274162-69274181	MS.gene72493:CDS	35.0%
	CAATGAGTGATGTACAAACT+TGG	+	chr4.2:69274072-69274091	MS.gene72493:CDS	35.0%
	TTGATGTCAACTCTCTCAAA+AGG	+	chr4.2:69273921-69273940	MS.gene72493:CDS	35.0%
	CCATTTCATCGATAGACCTT+CGG	-	chr4.2:69274022-69274041	None:intergenic	40.0%
	CGAAGGTCTATCGATGAAAT+GGG	+	chr4.2:69274020-69274039	MS.gene72493:CDS	40.0%
	GATCAAAACACTTGTACCGA+CGG	+	chr4.2:69274113-69274132	MS.gene72493:CDS	40.0%
	TACGGAGAATGTTAAGACAG+TGG	+	chr4.2:69274154-69274173	MS.gene72493:CDS	40.0%
	TCAACTCTCTCAAAAGGTCA+GGG	+	chr4.2:69273927-69273946	MS.gene72493:CDS	40.0%
	CAACTCTCTCAAAAGGTCAG+GGG	+	chr4.2:69273928-69273947	MS.gene72493:CDS	45.0%
	CCGAAGGTCTATCGATGAAA+TGG	+	chr4.2:69274019-69274038	MS.gene72493:CDS	45.0%
	GTCAACTCTCTCAAAAGGTC+AGG	+	chr4.2:69273926-69273945	MS.gene72493:CDS	45.0%
!	TGCATCGGCAAAATCAACCT+CGG	+	chr4.2:69273896-69273915	MS.gene72493:CDS	45.0%
!!	TTGGGTTAGTGCTGCTTTGA+CGG	+	chr4.2:69274091-69274110	MS.gene72493:CDS	45.0%
	AAGAGAGGCTGCAGCTATGA+AGG	+	chr4.2:69273959-69273978	MS.gene72493:CDS	50.0%
	AGACCTTCGGAGTTCATCGA+CGG	-	chr4.2:69274009-69274028	None:intergenic	50.0%
	AGCTATGAAGGATTGCGTCG+AGG	+	chr4.2:69273971-69273990	MS.gene72493:CDS	50.0%
	ATTCCGTCGATGAACTCCGA+AGG	+	chr4.2:69274003-69274022	MS.gene72493:CDS	50.0%
	CCTCAACGTGACTCTTGCAT+CGG	+	chr4.2:69273881-69273900	MS.gene72493:CDS	50.0%
	CGATGCAAGAGTCACGTTGA+GGG	-	chr4.2:69273883-69273902	None:intergenic	50.0%
	GAGAGTTGACATCAAAGCCG+AGG	-	chr4.2:69273916-69273935	None:intergenic	50.0%
	TCATAGCTGCAGCCTCTCTT+GGG	-	chr4.2:69273959-69273978	None:intergenic	50.0%
	TTCATAGCTGCAGCCTCTCT+TGG	-	chr4.2:69273960-69273979	None:intergenic	50.0%
	CCGATGCAAGAGTCACGTTG+AGG	-	chr4.2:69273884-69273903	None:intergenic	55.0%
	TCAGGGGTTGAACCCAAGAG+AGG	+	chr4.2:69273944-69273963	MS.gene72493:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	69273861	69274259	69273861	ID=MS.gene72493
chr4.2	mRNA	69273861	69274259	69273861	ID=MS.gene72493.t1;Parent=MS.gene72493
chr4.2	exon	69273861	69274259	69273861	ID=MS.gene72493.t1.exon1;Parent=MS.gene72493.t1
chr4.2	CDS	69273861	69274259	69273861	ID=cds.MS.gene72493.t1;Parent=MS.gene72493.t1

Gene Sequence

>MS.gene72493

ATGCTTTTAACAGGAACGGCCCTCAACGTGACTCTTGCATCGGCAAAATCAACCTCGGCTTTGATGTCAACTCTCTCAAAAGGTCAGGGGTTGAACCCAAGAGAGGCTGCAGCTATGAAGGATTGCGTCGAGGTGCTAAGTGATTCCGTCGATGAACTCCGAAGGTCTATCGATGAAATGGGCCGTCTTAGTACATCAAATTTTGATCTAACAATGAGTGATGTACAAACTTGGGTTAGTGCTGCTTTGACGGATCAAAACACTTGTACCGACGGATTTCAAGAGATTAGTGCTACGGAGAATGTTAAGACAGTGGTAAGAGGGAGCATTGTGCAAGTTGCTCAATTGACTAGCAATGCTTTAGCTTTGATAAATAAACTTTCCACTTCACAAGGTTAA

Protein sequence

>MS.gene72493.t1

MLLTGTALNVTLASAKSTSALMSTLSKGQGLNPREAAAMKDCVEVLSDSVDELRRSIDEMGRLSTSNFDLTMSDVQTWVSAALTDQNTCTDGFQEISATENVKTVVRGSIVQVAQLTSNALALINKLSTSQG