Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00955.t1 | XP_003594049.2 | 99.3 | 153 | 1 | 0 | 1 | 153 | 1 | 153 | 1.20E-78 | 302.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00955.t1 | Q9ZNV9 | 71.1 | 152 | 44 | 0 | 1 | 152 | 1 | 152 | 3.0e-57 | 222.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00955.t1 | B7FGU6 | 99.3 | 153 | 1 | 0 | 1 | 153 | 1 | 153 | 8.8e-79 | 302.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00955 | MS.gene83535 | PPI |
| MS.gene89014 | MS.gene00955 | PPI |
| MS.gene75348 | MS.gene00955 | PPI |
| MS.gene026751 | MS.gene00955 | PPI |
| MS.gene034089 | MS.gene00955 | PPI |
| MS.gene60023 | MS.gene00955 | PPI |
| MS.gene067406 | MS.gene00955 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00955.t1 | MTR_2g020770 | 99.346 | 153 | 1 | 0 | 1 | 153 | 1 | 153 | 5.93e-111 | 311 |
| MS.gene00955.t1 | MTR_2g020770 | 96.429 | 140 | 5 | 0 | 1 | 140 | 1 | 140 | 2.35e-97 | 277 |
| MS.gene00955.t1 | MTR_1g082290 | 56.835 | 139 | 60 | 0 | 5 | 143 | 6 | 144 | 1.09e-59 | 182 |
| MS.gene00955.t1 | MTR_7g114020 | 60.993 | 141 | 54 | 1 | 9 | 148 | 6 | 146 | 4.28e-59 | 180 |
| MS.gene00955.t1 | MTR_1g089130 | 55.944 | 143 | 63 | 0 | 5 | 147 | 3 | 145 | 1.06e-56 | 174 |
| MS.gene00955.t1 | MTR_2g100880 | 42.446 | 139 | 80 | 0 | 1 | 139 | 1 | 139 | 5.20e-39 | 129 |
| MS.gene00955.t1 | MTR_2g100900 | 42.446 | 139 | 80 | 0 | 1 | 139 | 1 | 139 | 9.47e-39 | 129 |
| MS.gene00955.t1 | MTR_2g103870 | 46.207 | 145 | 73 | 3 | 10 | 149 | 10 | 154 | 1.79e-31 | 110 |
| MS.gene00955.t1 | MTR_4g010160 | 43.478 | 115 | 63 | 2 | 37 | 149 | 28 | 142 | 7.56e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00955.t1 | AT3G21510 | 70.588 | 153 | 45 | 0 | 1 | 153 | 1 | 153 | 6.73e-71 | 210 |
| MS.gene00955.t1 | AT1G03430 | 52.632 | 152 | 71 | 1 | 3 | 153 | 6 | 157 | 7.81e-56 | 172 |
| MS.gene00955.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 5 | 155 | 3.76e-51 | 160 |
| MS.gene00955.t1 | AT3G29350 | 48.026 | 152 | 78 | 1 | 3 | 153 | 5 | 156 | 4.67e-51 | 160 |
| MS.gene00955.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 38 | 188 | 1.44e-50 | 160 |
| MS.gene00955.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 54 | 204 | 3.23e-50 | 160 |
| MS.gene00955.t1 | AT5G39340 | 50.331 | 151 | 74 | 1 | 3 | 152 | 54 | 204 | 3.23e-50 | 160 |
| MS.gene00955.t1 | AT1G03430 | 51.852 | 135 | 64 | 1 | 3 | 136 | 6 | 140 | 1.82e-46 | 149 |
| MS.gene00955.t1 | AT3G16360 | 44.030 | 134 | 75 | 0 | 6 | 139 | 1 | 134 | 8.65e-39 | 129 |
| MS.gene00955.t1 | AT3G16360 | 45.000 | 120 | 66 | 0 | 19 | 138 | 8 | 127 | 1.04e-36 | 123 |
| MS.gene00955.t1 | AT3G29350 | 47.273 | 110 | 57 | 1 | 3 | 111 | 5 | 114 | 6.43e-34 | 115 |
| MS.gene00955.t1 | AT1G80100 | 49.275 | 138 | 68 | 2 | 14 | 149 | 17 | 154 | 2.32e-27 | 100 |
| MS.gene00955.t1 | AT1G80100 | 47.778 | 90 | 46 | 1 | 61 | 149 | 43 | 132 | 1.21e-21 | 84.7 |
| MS.gene00955.t1 | AT4G04402 | 33.750 | 160 | 94 | 4 | 6 | 153 | 8 | 167 | 1.32e-21 | 85.9 |
| MS.gene00955.t1 | AT1G80100 | 46.774 | 124 | 65 | 1 | 14 | 136 | 17 | 140 | 1.51e-21 | 85.5 |
Find 30 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACATGTTCACCAGTTTA+AGG | 0.220882 | 2.3:+66517551 | MS.gene00955:CDS |
| ATGGTGAACAAAGGGTTTCT+TGG | 0.350647 | 2.3:+66517099 | MS.gene00955:intron |
| ACTCAGTTCAGTTCCATCAT+AGG | 0.355461 | 2.3:-66518239 | None:intergenic |
| GCTGCTGAAGTTGGAGAAAC+TGG | 0.408482 | 2.3:-66517126 | None:intergenic |
| TTGCACTGCTACCCTTAAAC+TGG | 0.425560 | 2.3:-66517563 | None:intergenic |
| GCACATGTTCACCAGTTTAA+GGG | 0.428803 | 2.3:+66517552 | MS.gene00955:CDS |
| CTACATCAAATCCATGTTCA+TGG | 0.459422 | 2.3:+66516947 | MS.gene00955:CDS |
| CTAATTTATGCAGGTGCCGC+AGG | 0.459692 | 2.3:+66518022 | MS.gene00955:intron |
| CATCTTGTAGCTGCTGAAGT+TGG | 0.465876 | 2.3:-66517135 | None:intergenic |
| GCAGCTTGAGCAGCAGATAG+TGG | 0.473916 | 2.3:+66518199 | MS.gene00955:intron |
| CTCAGTTCAGTTCCATCATA+GGG | 0.476361 | 2.3:-66518238 | None:intergenic |
| GGGTCAGATGCGGAGACAGT+GGG | 0.506261 | 2.3:+66516920 | MS.gene00955:CDS |
| TAAGCTTGAAACATTGTTGA+GGG | 0.522076 | 2.3:+66518086 | MS.gene00955:CDS |
| TGAACAAAGGGTTTCTTGGA+TGG | 0.522720 | 2.3:+66517103 | MS.gene00955:intron |
| TGACAGCATAGGCGCACAAA+GGG | 0.523421 | 2.3:+66517773 | MS.gene00955:intron |
| GAGCAGCAGATAGTGGCAGC+TGG | 0.537850 | 2.3:+66518206 | MS.gene00955:CDS |
| TGGTCTAACCTCCATGAACA+TGG | 0.544818 | 2.3:-66516958 | None:intergenic |
| TGGTGGCTCAATCCCTATGA+TGG | 0.558041 | 2.3:+66518226 | MS.gene00955:CDS |
| TTTGTAGAGAAACATGGAGG+TGG | 0.564605 | 2.3:+66516899 | None:intergenic |
| ATAAGCTTGAAACATTGTTG+AGG | 0.584829 | 2.3:+66518085 | MS.gene00955:CDS |
| TGGGTCAGATGCGGAGACAG+TGG | 0.592973 | 2.3:+66516919 | MS.gene00955:CDS |
| TCAGATGCGGAGACAGTGGG+TGG | 0.608143 | 2.3:+66516923 | MS.gene00955:CDS |
| TTGTAGAGAAACATGGAGGT+GGG | 0.611758 | 2.3:+66516900 | None:intergenic |
| AGTTTAAGGGTAGCAGTGCA+AGG | 0.619461 | 2.3:+66517565 | MS.gene00955:CDS |
| TCACTTGCTGCAAACACCTG+CGG | 0.622315 | 2.3:-66518038 | None:intergenic |
| CAGCAGATAGTGGCAGCTGG+TGG | 0.634128 | 2.3:+66518209 | MS.gene00955:CDS |
| GAAATTGCGAAAAGCAACGC+AGG | 0.637314 | 2.3:-66517805 | None:intergenic |
| ACATGGAGGTGGGTCAGATG+CGG | 0.639294 | 2.3:+66516910 | None:intergenic |
| TTGACAGCATAGGCGCACAA+AGG | 0.651584 | 2.3:+66517772 | MS.gene00955:intron |
| CATCAAATCCATGTTCATGG+AGG | 0.716249 | 2.3:+66516950 | MS.gene00955:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTATATGATTTCTTTTA+TGG | + | chr2.3:66518167-66518186 | MS.gene00955:intron | 10.0% |
| !! | AAATCCATAAGTTTAATATA+AGG | - | chr2.3:66518145-66518164 | None:intergenic | 15.0% |
| !! | ATTAGAGAATATAATGAATT+TGG | + | chr2.3:66517296-66517315 | MS.gene00955:intron | 15.0% |
| !! | AACAAGTGAAAAAACATAAA+TGG | - | chr2.3:66517699-66517718 | None:intergenic | 20.0% |
| !! | AATAAAGTATAACCAAGAAT+TGG | - | chr2.3:66517393-66517412 | None:intergenic | 20.0% |
| !! | AGAACCTTATATTAAACTTA+TGG | + | chr2.3:66518138-66518157 | MS.gene00955:intron | 20.0% |
| !!! | TTGTTTTTCTGTTTTGTTTA+TGG | + | chr2.3:66517080-66517099 | MS.gene00955:intron | 20.0% |
| !!! | TTTTCAATTGTTGATTTTGT+TGG | + | chr2.3:66517054-66517073 | MS.gene00955:intron | 20.0% |
| !!! | TTTTGCTTTTGTTTTTGTTT+AGG | + | chr2.3:66517021-66517040 | MS.gene00955:intron | 20.0% |
| ! | AACATGAGAGAAATGAAAAA+AGG | - | chr2.3:66517272-66517291 | None:intergenic | 25.0% |
| ! | ACAACTTCAAAAACAAATTC+AGG | - | chr2.3:66517169-66517188 | None:intergenic | 25.0% |
| ! | ACCAAAAATATATAGTAGCA+TGG | - | chr2.3:66517726-66517745 | None:intergenic | 25.0% |
| ! | CTGTAGCATTTATACATTTA+AGG | - | chr2.3:66517613-66517632 | None:intergenic | 25.0% |
| ! | GTGAAATTTCTAATTTATGC+AGG | + | chr2.3:66518013-66518032 | MS.gene00955:intron | 25.0% |
| !! | ACCATGCTACTATATATTTT+TGG | + | chr2.3:66517722-66517741 | MS.gene00955:intron | 25.0% |
| !! | AGCATTTATACATTTAAGGT+TGG | - | chr2.3:66517609-66517628 | None:intergenic | 25.0% |
| !! | TGAATTTTTCGCTAATTTCA+TGG | + | chr2.3:66517981-66518000 | MS.gene00955:intron | 25.0% |
| !!! | GACTAGTTGTTTAATAGTTT+TGG | + | chr2.3:66517869-66517888 | MS.gene00955:intron | 25.0% |
| !!! | TTTTGTTTATGGTGAACAAA+GGG | + | chr2.3:66517091-66517110 | MS.gene00955:intron | 25.0% |
| AAGTATAACCAAGAATTGGT+TGG | - | chr2.3:66517389-66517408 | None:intergenic | 30.0% | |
| AGACATGATCTAATGTTGTT+GGG | + | chr2.3:66517480-66517499 | MS.gene00955:intron | 30.0% | |
| ATAAGCTTGAAACATTGTTG+AGG | + | chr2.3:66518085-66518104 | MS.gene00955:CDS | 30.0% | |
| GCTACAGAATCTGAATAAAT+CGG | + | chr2.3:66517626-66517645 | MS.gene00955:intron | 30.0% | |
| TAAGCTTGAAACATTGTTGA+GGG | + | chr2.3:66518086-66518105 | MS.gene00955:CDS | 30.0% | |
| ! | ACACATTAATTGACTCTTCT+GGG | + | chr2.3:66516987-66517006 | MS.gene00955:intron | 30.0% |
| ! | GTCAATTAATGTGTGTTTTG+TGG | - | chr2.3:66516981-66517000 | None:intergenic | 30.0% |
| !! | TTTTCTTTGATGATTCTGAG+AGG | + | chr2.3:66517194-66517213 | MS.gene00955:CDS | 30.0% |
| !!! | GTTTTGTTTATGGTGAACAA+AGG | + | chr2.3:66517090-66517109 | MS.gene00955:intron | 30.0% |
| CTACATCAAATCCATGTTCA+TGG | + | chr2.3:66516947-66516966 | MS.gene00955:CDS | 35.0% | |
| GACATGATCTAATGTTGTTG+GGG | + | chr2.3:66517481-66517500 | MS.gene00955:intron | 35.0% | |
| GAGACATGATCTAATGTTGT+TGG | + | chr2.3:66517479-66517498 | MS.gene00955:intron | 35.0% | |
| GTATGTTTGATTGACAGCAT+AGG | + | chr2.3:66517762-66517781 | MS.gene00955:intron | 35.0% | |
| TCTCCTGAATGATTCATTCT+AGG | - | chr2.3:66517351-66517370 | None:intergenic | 35.0% | |
| ! | CACACATTAATTGACTCTTC+TGG | + | chr2.3:66516986-66517005 | MS.gene00955:intron | 35.0% |
| ATGGTGAACAAAGGGTTTCT+TGG | + | chr2.3:66517099-66517118 | MS.gene00955:intron | 40.0% | |
| CAGCTAGTGCAATAGTTTGA+AGG | + | chr2.3:66517908-66517927 | MS.gene00955:intron | 40.0% | |
| CATCAAATCCATGTTCATGG+AGG | + | chr2.3:66516950-66516969 | MS.gene00955:CDS | 40.0% | |
| CCACCTAGAATGAATCATTC+AGG | + | chr2.3:66517345-66517364 | MS.gene00955:intron | 40.0% | |
| CCTGAATGATTCATTCTAGG+TGG | - | chr2.3:66517348-66517367 | None:intergenic | 40.0% | |
| CTTTACTGCTTAGATGCTAG+AGG | + | chr2.3:66517950-66517969 | MS.gene00955:intron | 40.0% | |
| GCACATGTTCACCAGTTTAA+GGG | + | chr2.3:66517552-66517571 | MS.gene00955:CDS | 40.0% | |
| TGAACAAAGGGTTTCTTGGA+TGG | + | chr2.3:66517103-66517122 | MS.gene00955:intron | 40.0% | |
| TGCACATGTTCACCAGTTTA+AGG | + | chr2.3:66517551-66517570 | MS.gene00955:CDS | 40.0% | |
| !! | CTCTCTTGCTATTAATCACC+CGG | - | chr2.3:66517460-66517479 | None:intergenic | 40.0% |
| AGTTTAAGGGTAGCAGTGCA+AGG | + | chr2.3:66517565-66517584 | MS.gene00955:CDS | 45.0% | |
| ATCATTCAGGAGAACCCTCT+TGG | + | chr2.3:66517358-66517377 | MS.gene00955:intron | 45.0% | |
| CATCTTGTAGCTGCTGAAGT+TGG | - | chr2.3:66517138-66517157 | None:intergenic | 45.0% | |
| CTATTAATCACCCGGTTCTG+GGG | - | chr2.3:66517452-66517471 | None:intergenic | 45.0% | |
| GAAATTGCGAAAAGCAACGC+AGG | - | chr2.3:66517808-66517827 | None:intergenic | 45.0% | |
| GCTATTAATCACCCGGTTCT+GGG | - | chr2.3:66517453-66517472 | None:intergenic | 45.0% | |
| TCATTCAGGAGAACCCTCTT+GGG | + | chr2.3:66517359-66517378 | MS.gene00955:intron | 45.0% | |
| TGCTATTAATCACCCGGTTC+TGG | - | chr2.3:66517454-66517473 | None:intergenic | 45.0% | |
| TGGTCTAACCTCCATGAACA+TGG | - | chr2.3:66516961-66516980 | None:intergenic | 45.0% | |
| TTGCACTGCTACCCTTAAAC+TGG | - | chr2.3:66517566-66517585 | None:intergenic | 45.0% | |
| TTTACCTCTCGAGCGAACTT+CGG | + | chr2.3:66517411-66517430 | MS.gene00955:intron | 45.0% | |
| ! | GTTGGGGTTTTACATGTGCA+GGG | + | chr2.3:66517497-66517516 | MS.gene00955:intron | 45.0% |
| ! | TGTTGGGGTTTTACATGTGC+AGG | + | chr2.3:66517496-66517515 | MS.gene00955:intron | 45.0% |
| GCTGCTGAAGTTGGAGAAAC+TGG | - | chr2.3:66517129-66517148 | None:intergenic | 50.0% | |
| TAATCCGAAGTTCGCTCGAG+AGG | - | chr2.3:66517418-66517437 | None:intergenic | 50.0% | |
| TGACAGCATAGGCGCACAAA+GGG | + | chr2.3:66517773-66517792 | MS.gene00955:intron | 50.0% | |
| TGGGTGGACCAACCAATTCT+TGG | + | chr2.3:66517378-66517397 | MS.gene00955:intron | 50.0% | |
| TGGTGGCTCAATCCCTATGA+TGG | + | chr2.3:66518226-66518245 | MS.gene00955:CDS | 50.0% | |
| TTGACAGCATAGGCGCACAA+AGG | + | chr2.3:66517772-66517791 | MS.gene00955:intron | 50.0% | |
| ! | AATTGGTTGGTCCACCCAAG+AGG | - | chr2.3:66517376-66517395 | None:intergenic | 50.0% |
| ! | ATTGGTTGGTCCACCCAAGA+GGG | - | chr2.3:66517375-66517394 | None:intergenic | 50.0% |
| ! | CTAATTTATGCAGGTGCCGC+AGG | + | chr2.3:66518022-66518041 | MS.gene00955:intron | 50.0% |
| ! | TCACTTGCTGCAAACACCTG+CGG | - | chr2.3:66518041-66518060 | None:intergenic | 50.0% |
| CGAGCGAACTTCGGATTACC+AGG | + | chr2.3:66517420-66517439 | MS.gene00955:intron | 55.0% | |
| GCAGCTTGAGCAGCAGATAG+TGG | + | chr2.3:66518199-66518218 | MS.gene00955:intron | 55.0% | |
| TTCAGGAGAACCCTCTTGGG+TGG | + | chr2.3:66517362-66517381 | MS.gene00955:intron | 55.0% | |
| !! | AATCACCCGGTTCTGGGGAA+GGG | - | chr2.3:66517447-66517466 | None:intergenic | 55.0% |
| !! | TAATCACCCGGTTCTGGGGA+AGG | - | chr2.3:66517448-66517467 | None:intergenic | 55.0% |
| GGGTCAGATGCGGAGACAGT+GGG | + | chr2.3:66516920-66516939 | MS.gene00955:CDS | 60.0% | |
| TCAGATGCGGAGACAGTGGG+TGG | + | chr2.3:66516923-66516942 | MS.gene00955:CDS | 60.0% | |
| TGGGTCAGATGCGGAGACAG+TGG | + | chr2.3:66516919-66516938 | MS.gene00955:CDS | 60.0% | |
| ! | CAGCAGATAGTGGCAGCTGG+TGG | + | chr2.3:66518209-66518228 | MS.gene00955:CDS | 60.0% |
| ! | GAGCAGCAGATAGTGGCAGC+TGG | + | chr2.3:66518206-66518225 | MS.gene00955:CDS | 60.0% |
| !! | ATCACCCGGTTCTGGGGAAG+GGG | - | chr2.3:66517446-66517465 | None:intergenic | 60.0% |
| CAGGCCCCTTCCCCAGAACC+GGG | + | chr2.3:66517439-66517458 | MS.gene00955:intron | 70.0% | |
| CCAGGCCCCTTCCCCAGAAC+CGG | + | chr2.3:66517438-66517457 | MS.gene00955:intron | 70.0% | |
| !! | CCGGTTCTGGGGAAGGGGCC+TGG | - | chr2.3:66517441-66517460 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 66516912 | 66518259 | 66516912 | ID=MS.gene00955 |
| chr2.3 | mRNA | 66516912 | 66518259 | 66516912 | ID=MS.gene00955.t1;Parent=MS.gene00955 |
| chr2.3 | exon | 66516912 | 66516971 | 66516912 | ID=MS.gene00955.t1.exon1;Parent=MS.gene00955.t1 |
| chr2.3 | CDS | 66516912 | 66516971 | 66516912 | ID=cds.MS.gene00955.t1;Parent=MS.gene00955.t1 |
| chr2.3 | exon | 66517112 | 66517242 | 66517112 | ID=MS.gene00955.t1.exon2;Parent=MS.gene00955.t1 |
| chr2.3 | CDS | 66517112 | 66517242 | 66517112 | ID=cds.MS.gene00955.t1;Parent=MS.gene00955.t1 |
| chr2.3 | exon | 66517518 | 66517586 | 66517518 | ID=MS.gene00955.t1.exon3;Parent=MS.gene00955.t1 |
| chr2.3 | CDS | 66517518 | 66517586 | 66517518 | ID=cds.MS.gene00955.t1;Parent=MS.gene00955.t1 |
| chr2.3 | exon | 66517779 | 66517850 | 66517779 | ID=MS.gene00955.t1.exon4;Parent=MS.gene00955.t1 |
| chr2.3 | CDS | 66517779 | 66517850 | 66517779 | ID=cds.MS.gene00955.t1;Parent=MS.gene00955.t1 |
| chr2.3 | exon | 66518035 | 66518107 | 66518035 | ID=MS.gene00955.t1.exon5;Parent=MS.gene00955.t1 |
| chr2.3 | CDS | 66518035 | 66518107 | 66518035 | ID=cds.MS.gene00955.t1;Parent=MS.gene00955.t1 |
| chr2.3 | exon | 66518203 | 66518259 | 66518203 | ID=MS.gene00955.t1.exon6;Parent=MS.gene00955.t1 |
| chr2.3 | CDS | 66518203 | 66518259 | 66518203 | ID=cds.MS.gene00955.t1;Parent=MS.gene00955.t1 |
| Gene Sequence |
| Protein sequence |
