Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010130.t1 | XP_024639642.1 | 56.8 | 806 | 246 | 9 | 31 | 737 | 35 | 837 | 2.20E-136 | 496.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010130.t1 | P61430 | 33.5 | 775 | 413 | 11 | 31 | 710 | 28 | 795 | 8.3e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010130.t1 | G7KB78 | 53.0 | 843 | 253 | 11 | 31 | 737 | 35 | 870 | 2.9e-122 | 449.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene81353 | MS.gene010130 | PPI |
| MS.gene010130 | MS.gene82770 | PPI |
| MS.gene010130 | MS.gene032693 | PPI |
| MS.gene63911 | MS.gene010130 | PPI |
| MS.gene010130 | MS.gene27815 | PPI |
| MS.gene20859 | MS.gene010130 | PPI |
| MS.gene020750 | MS.gene010130 | PPI |
| MS.gene016661 | MS.gene010130 | PPI |
| MS.gene010130 | MS.gene45755 | PPI |
| MS.gene07298 | MS.gene010130 | PPI |
| MS.gene010130 | MS.gene20809 | PPI |
| MS.gene053106 | MS.gene010130 | PPI |
| MS.gene010130 | MS.gene058851 | PPI |
| MS.gene023588 | MS.gene010130 | PPI |
| MS.gene005513 | MS.gene010130 | PPI |
| MS.gene010130 | MS.gene95260 | PPI |
| MS.gene010130 | MS.gene07294 | PPI |
| MS.gene010130 | MS.gene056175 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010130.t1 | MTR_5g063730 | 79.416 | 787 | 56 | 11 | 50 | 737 | 91 | 870 | 0.0 | 1144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010130.t1 | AT1G22260 | 41.048 | 821 | 366 | 15 | 31 | 740 | 28 | 841 | 1.47e-165 | 500 |
| MS.gene010130.t1 | AT1G22275 | 40.988 | 810 | 379 | 16 | 21 | 737 | 20 | 823 | 3.66e-163 | 493 |
| MS.gene010130.t1 | AT1G22260 | 40.610 | 820 | 371 | 13 | 31 | 740 | 28 | 841 | 1.52e-161 | 489 |
| MS.gene010130.t1 | AT1G22275 | 41.191 | 789 | 366 | 15 | 21 | 717 | 20 | 802 | 1.86e-158 | 480 |
| MS.gene010130.t1 | AT1G22260 | 39.897 | 777 | 347 | 16 | 76 | 740 | 1 | 769 | 1.26e-146 | 449 |
| MS.gene010130.t1 | AT1G22275 | 39.036 | 643 | 303 | 11 | 21 | 579 | 20 | 657 | 2.54e-107 | 342 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTTCTCCTCTAATT+TGG | 0.132309 | 5.2:+55526986 | None:intergenic |
| TATTCGATACTTTGGATTTC+TGG | 0.140046 | 5.2:+55525405 | None:intergenic |
| TTGATTCTTCTTTGCACTTT+TGG | 0.140775 | 5.2:+55526129 | None:intergenic |
| TTAACTTCTGGGTCCTCTTT+TGG | 0.227517 | 5.2:+55525266 | None:intergenic |
| ATTACCTTAACAACAGTTCT+AGG | 0.252326 | 5.2:+55522567 | None:intergenic |
| CATAGCCAGTGAACAGAAAT+TGG | 0.268411 | 5.2:-55527470 | MS.gene010130:intron |
| AGGCTATTAATGACATTAAA+AGG | 0.292046 | 5.2:-55526309 | MS.gene010130:CDS |
| GACTGAAAGCAAAGAAAATA+TGG | 0.298375 | 5.2:-55526812 | MS.gene010130:CDS |
| AGTCACAATTTCCAAATTAG+AGG | 0.303525 | 5.2:-55526997 | MS.gene010130:CDS |
| TTTCACTGTGTTCAGCTTGC+AGG | 0.305499 | 5.2:+55525675 | None:intergenic |
| ATTAAAAGAAGGAGTTCTTT+TGG | 0.305809 | 5.2:-55523179 | MS.gene010130:CDS |
| TCGTTGTACGGTTCAACTTC+GGG | 0.306730 | 5.2:-55530390 | MS.gene010130:CDS |
| ATGCTTTAATCTCAGCTTCA+TGG | 0.331628 | 5.2:+55525480 | None:intergenic |
| AAGTCTCAACTTCATATTCA+TGG | 0.337259 | 5.2:+55522823 | None:intergenic |
| CATGTTAACTTCTAGTAGAT+TGG | 0.371843 | 5.2:-55527569 | MS.gene010130:CDS |
| GTCTTCTAAATTCTTTATCA+TGG | 0.377090 | 5.2:+55527591 | None:intergenic |
| CTCTGTAGAATCTAGCTTCA+AGG | 0.380220 | 5.2:+55529593 | None:intergenic |
| TATTATGAAGCTCATCATAC+TGG | 0.387315 | 5.2:+55527210 | None:intergenic |
| GAAGTATGAATTAGAGAAGA+TGG | 0.391033 | 5.2:-55526287 | MS.gene010130:CDS |
| ATCGTTGTACGGTTCAACTT+CGG | 0.402876 | 5.2:-55530391 | MS.gene010130:CDS |
| TAACGAAAATGCGAAGCTTA+AGG | 0.403071 | 5.2:-55529923 | MS.gene010130:intron |
| CCCAGAAGTTAATTCAAAAC+AGG | 0.404035 | 5.2:-55525255 | MS.gene010130:intron |
| TGAAATTTGCTGCTGAAGAA+AGG | 0.404224 | 5.2:-55529427 | MS.gene010130:CDS |
| CAATCTTGAAGGATCACTTC+AGG | 0.408264 | 5.2:-55527371 | MS.gene010130:CDS |
| ATTCCACCATCAGAGGTTAA+AGG | 0.411987 | 5.2:+55521613 | None:intergenic |
| ACAGCTTCGGAATGTCTTAG+AGG | 0.416291 | 5.2:-55526683 | MS.gene010130:CDS |
| TATGAAGTTGAGACTTCTAA+TGG | 0.417404 | 5.2:-55522816 | MS.gene010130:CDS |
| GAAGCAGTTGATACACATTC+AGG | 0.420127 | 5.2:-55526105 | MS.gene010130:CDS |
| TTCTAAACACTGCAGCGATT+CGG | 0.428408 | 5.2:-55521677 | MS.gene010130:intron |
| TACCCCTAGAACTGTTGTTA+AGG | 0.431512 | 5.2:-55522571 | MS.gene010130:intron |
| TGATCAACTATAATACCATT+TGG | 0.435411 | 5.2:+55526931 | None:intergenic |
| TTCTTCAACAACTTCGGCAC+TGG | 0.436472 | 5.2:+55523008 | None:intergenic |
| TGCAAATGTTATAGAAAAGA+AGG | 0.438541 | 5.2:-55529372 | MS.gene010130:CDS |
| CACTTTGCAGCATAAAGAAC+GGG | 0.451299 | 5.2:-55526647 | MS.gene010130:CDS |
| CAGAAAATAAACAATCTTGA+AGG | 0.457143 | 5.2:-55527382 | MS.gene010130:CDS |
| TTACCTTAACAACAGTTCTA+GGG | 0.457211 | 5.2:+55522568 | None:intergenic |
| TCTTCAGATTCCGTTTCATC+GGG | 0.458626 | 5.2:-55530328 | MS.gene010130:CDS |
| ATCTTCAGATTCCGTTTCAT+CGG | 0.462299 | 5.2:-55530329 | MS.gene010130:CDS |
| TGCTGAAAGAATTCAGCGTT+TGG | 0.463548 | 5.2:-55527063 | MS.gene010130:CDS |
| ACTCTGCTACAGTGGACTCT+TGG | 0.466070 | 5.2:+55527108 | None:intergenic |
| CGCGAAATTTCCCGATGAAA+CGG | 0.468531 | 5.2:+55530318 | None:intergenic |
| TGTAGAAAACAATGTTTATG+AGG | 0.473803 | 5.2:-55525508 | MS.gene010130:intron |
| AGTTTGAATGAACAGATGAA+TGG | 0.475445 | 5.2:-55529621 | MS.gene010130:CDS |
| GAAGAACAATTACAAAGAAA+AGG | 0.477258 | 5.2:-55522794 | MS.gene010130:CDS |
| TAGAAATCAATACGATCAAA+TGG | 0.477543 | 5.2:-55526468 | MS.gene010130:intron |
| GAAATTTGCTGCTGAAGAAA+GGG | 0.478421 | 5.2:-55529426 | MS.gene010130:CDS |
| GTATCACCTTTAACCTCTGA+TGG | 0.480455 | 5.2:-55521619 | None:intergenic |
| ACAAGGAAGTCCTACAGCTT+CGG | 0.480664 | 5.2:-55526696 | MS.gene010130:CDS |
| GGAAATTGATACTCTGCAAA+AGG | 0.483387 | 5.2:-55526755 | MS.gene010130:CDS |
| TGAAGCTTTAATCTCAAAGA+AGG | 0.488627 | 5.2:-55527030 | MS.gene010130:CDS |
| TCCGCTTTCTTCAACAACTT+CGG | 0.488814 | 5.2:+55523002 | None:intergenic |
| AGAAAGTGTCTCTTTCGTGC+TGG | 0.501908 | 5.2:+55527249 | None:intergenic |
| CCGTTTCATCAATTAAAAGA+AGG | 0.503750 | 5.2:-55523190 | MS.gene010130:CDS |
| CAGCAGCAGTTAATAATGAC+CGG | 0.503791 | 5.2:+55526510 | None:intergenic |
| TTCGTGTGCAGAGAAATTAG+TGG | 0.505393 | 5.2:-55530187 | MS.gene010130:intron |
| AGGCTTTGCTGCAGTTGCAA+TGG | 0.513603 | 5.2:-55525307 | MS.gene010130:CDS |
| AAGAAGTACTGTCTTGTTCG+AGG | 0.515969 | 5.2:-55522766 | MS.gene010130:intron |
| AAAAGTGCAAAGAAGAATCA+AGG | 0.516421 | 5.2:-55526127 | MS.gene010130:CDS |
| TAAAGAACGGGAAAAGAAGC+TGG | 0.519691 | 5.2:-55526635 | MS.gene010130:CDS |
| AGAAAATATGGAAAGAATGC+AGG | 0.520425 | 5.2:-55526800 | MS.gene010130:CDS |
| CTCAGTATACTCTGCTACAG+TGG | 0.526073 | 5.2:+55527100 | None:intergenic |
| GAAAACAGTTCTTGATATTG+TGG | 0.531568 | 5.2:-55530148 | MS.gene010130:intron |
| TGAAGGATCACTTCAGGAGA+TGG | 0.533658 | 5.2:-55527365 | MS.gene010130:CDS |
| GCTGATCTTGCTGAAAAGCT+TGG | 0.538124 | 5.2:-55527415 | MS.gene010130:CDS |
| CGATGATGATAACCGTTTCG+CGG | 0.538590 | 5.2:+55530365 | None:intergenic |
| TGCAGCAGGAACATGATAAG+AGG | 0.539558 | 5.2:-55525706 | MS.gene010130:CDS |
| TCAGGAGGAACACGCTAAGC+TGG | 0.546991 | 5.2:-55526087 | MS.gene010130:intron |
| GCGAATCTGAAGCTAACTGC+AGG | 0.547220 | 5.2:-55530298 | MS.gene010130:intron |
| CAACTTCGGGAACCGCGAAA+CGG | 0.550915 | 5.2:-55530377 | MS.gene010130:CDS |
| TTGAGGTGTGAACTTGAAGA+TGG | 0.552365 | 5.2:-55525458 | MS.gene010130:CDS |
| GATGAGTGACAAGCCAAAAG+AGG | 0.555709 | 5.2:-55525279 | MS.gene010130:CDS |
| CTAGGCTATTGCAGCAACTG+AGG | 0.565974 | 5.2:-55526162 | MS.gene010130:intron |
| GAGACTCTTCAACAGCTGAC+TGG | 0.566823 | 5.2:-55529819 | MS.gene010130:CDS |
| TTTCATCAAACTTGTTAACA+AGG | 0.574635 | 5.2:+55527305 | None:intergenic |
| ACAGGTTACTCATATGCAGC+AGG | 0.576637 | 5.2:-55525720 | MS.gene010130:intron |
| ATTAGTGGAAGAGCAAGCTT+CGG | 0.579996 | 5.2:-55530172 | MS.gene010130:CDS |
| GACAGATTCAAATCGTTGTA+CGG | 0.582183 | 5.2:-55530402 | MS.gene010130:CDS |
| AGTCAGCTGTTGAAGAGTCT+CGG | 0.587798 | 5.2:+55529821 | None:intergenic |
| TGGGTGTAGGAAGCTAGAAG+AGG | 0.588514 | 5.2:-55525438 | MS.gene010130:CDS |
| GCACTTTGCAGCATAAAGAA+CGG | 0.589489 | 5.2:-55526648 | MS.gene010130:CDS |
| GCCGAAGTTGTTGAAGAAAG+CGG | 0.596194 | 5.2:-55523003 | MS.gene010130:CDS |
| TAGGCTATTGCAGCAACTGA+GGG | 0.597211 | 5.2:-55526161 | MS.gene010130:CDS |
| GAAGATGGAGATTGTCAACA+TGG | 0.599410 | 5.2:-55526272 | MS.gene010130:CDS |
| CTAAGACATTCCGAAGCTGT+AGG | 0.605028 | 5.2:+55526686 | None:intergenic |
| TAGATCATCAGAGAACCAAA+TGG | 0.606718 | 5.2:-55526946 | MS.gene010130:CDS |
| GCAAATGTTATAGAAAAGAA+GGG | 0.606856 | 5.2:-55529371 | MS.gene010130:intron |
| CTGACTGGTATCGTTCAAGA+TGG | 0.609733 | 5.2:-55529804 | MS.gene010130:intron |
| TCGTGTTGTTTGATGCGTGT+AGG | 0.610324 | 5.2:+55521650 | None:intergenic |
| TACCTTAACAACAGTTCTAG+GGG | 0.611278 | 5.2:+55522569 | None:intergenic |
| AGACATATTGAGTAAATCAG+AGG | 0.616055 | 5.2:-55526776 | MS.gene010130:CDS |
| CTATTGCAGCAACTGAGGGA+AGG | 0.619754 | 5.2:-55526157 | MS.gene010130:CDS |
| AAGCTGAGATTAAAGCATTG+AGG | 0.623242 | 5.2:-55525475 | MS.gene010130:CDS |
| TTGATACTCTGCAAAAGGAG+AGG | 0.623737 | 5.2:-55526750 | MS.gene010130:CDS |
| GTGAACTTGAAGATGGGTGT+AGG | 0.643578 | 5.2:-55525451 | MS.gene010130:CDS |
| TGAGGTGTGAACTTGAAGAT+GGG | 0.664276 | 5.2:-55525457 | MS.gene010130:CDS |
| AGCTGAATATGAACTGAGAG+AGG | 0.693062 | 5.2:-55525630 | MS.gene010130:intron |
| GCAGTTGATACACATTCAGG+AGG | 0.702381 | 5.2:-55526102 | MS.gene010130:CDS |
| CACTGTAGCAGAGTATACTG+AGG | 0.703937 | 5.2:-55527099 | MS.gene010130:CDS |
| CTTGAAGCTAGATTCTACAG+AGG | 0.724886 | 5.2:-55529592 | MS.gene010130:CDS |
| GCTGGAAGATCAGATCACAG+AGG | 0.771101 | 5.2:-55526617 | MS.gene010130:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 55521622 | 55530452 | 55521622 | ID=MS.gene010130 |
| chr5.2 | mRNA | 55521622 | 55530452 | 55521622 | ID=MS.gene010130.t1;Parent=MS.gene010130 |
| chr5.2 | exon | 55530299 | 55530452 | 55530299 | ID=MS.gene010130.t1.exon1;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55530299 | 55530452 | 55530299 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55530149 | 55530198 | 55530149 | ID=MS.gene010130.t1.exon2;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55530149 | 55530198 | 55530149 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55529924 | 55530012 | 55529924 | ID=MS.gene010130.t1.exon3;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55529924 | 55530012 | 55529924 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55529805 | 55529860 | 55529805 | ID=MS.gene010130.t1.exon4;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55529805 | 55529860 | 55529805 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55529577 | 55529702 | 55529577 | ID=MS.gene010130.t1.exon5;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55529577 | 55529702 | 55529577 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55529372 | 55529470 | 55529372 | ID=MS.gene010130.t1.exon6;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55529372 | 55529470 | 55529372 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55527547 | 55527616 | 55527547 | ID=MS.gene010130.t1.exon7;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55527547 | 55527616 | 55527547 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55527316 | 55527483 | 55527316 | ID=MS.gene010130.t1.exon8;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55527316 | 55527483 | 55527316 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55526939 | 55527277 | 55526939 | ID=MS.gene010130.t1.exon9;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55526939 | 55527277 | 55526939 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55526618 | 55526867 | 55526618 | ID=MS.gene010130.t1.exon10;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55526618 | 55526867 | 55526618 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55526469 | 55526534 | 55526469 | ID=MS.gene010130.t1.exon11;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55526469 | 55526534 | 55526469 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55526261 | 55526329 | 55526261 | ID=MS.gene010130.t1.exon12;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55526261 | 55526329 | 55526261 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55526088 | 55526180 | 55526088 | ID=MS.gene010130.t1.exon13;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55526088 | 55526180 | 55526088 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55525631 | 55525738 | 55525631 | ID=MS.gene010130.t1.exon14;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55525631 | 55525738 | 55525631 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55525415 | 55525525 | 55525415 | ID=MS.gene010130.t1.exon15;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55525415 | 55525525 | 55525415 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55525256 | 55525342 | 55525256 | ID=MS.gene010130.t1.exon16;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55525256 | 55525342 | 55525256 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55523154 | 55523219 | 55523154 | ID=MS.gene010130.t1.exon17;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55523154 | 55523219 | 55523154 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55522998 | 55523060 | 55522998 | ID=MS.gene010130.t1.exon18;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55522998 | 55523060 | 55522998 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55522767 | 55522853 | 55522767 | ID=MS.gene010130.t1.exon19;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55522767 | 55522853 | 55522767 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55522572 | 55522631 | 55522572 | ID=MS.gene010130.t1.exon20;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55522572 | 55522631 | 55522572 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| chr5.2 | exon | 55521622 | 55521684 | 55521622 | ID=MS.gene010130.t1.exon21;Parent=MS.gene010130.t1 |
| chr5.2 | CDS | 55521622 | 55521684 | 55521622 | ID=cds.MS.gene010130.t1;Parent=MS.gene010130.t1 |
| Gene Sequence |
| Protein sequence |
