Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010234.t1 | XP_003615024.1 | 99 | 386 | 4 | 0 | 1 | 386 | 1 | 386 | 3.50E-223 | 783.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010234.t1 | Q803I2 | 52.9 | 393 | 161 | 4 | 5 | 386 | 4 | 383 | 6.2e-120 | 432.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010234.t1 | G7KA80 | 99.0 | 386 | 4 | 0 | 1 | 386 | 1 | 386 | 2.5e-223 | 783.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene69767 | MS.gene010234 | PPI |
| MS.gene70505 | MS.gene010234 | PPI |
| MS.gene044295 | MS.gene010234 | PPI |
| MS.gene25743 | MS.gene010234 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010234.t1 | MTR_5g062720 | 98.964 | 386 | 4 | 0 | 1 | 386 | 1 | 386 | 0.0 | 800 |
| MS.gene010234.t1 | MTR_5g062720 | 98.680 | 303 | 4 | 0 | 1 | 303 | 1 | 303 | 0.0 | 632 |
| MS.gene010234.t1 | MTR_4g094255 | 68.052 | 385 | 121 | 1 | 1 | 385 | 1 | 383 | 0.0 | 591 |
| MS.gene010234.t1 | MTR_4g094255 | 66.216 | 296 | 98 | 1 | 90 | 385 | 1 | 294 | 2.64e-158 | 447 |
| MS.gene010234.t1 | MTR_3g081480 | 36.729 | 373 | 205 | 7 | 8 | 378 | 7 | 350 | 2.54e-76 | 240 |
| MS.gene010234.t1 | MTR_4g080800 | 32.683 | 205 | 97 | 9 | 202 | 384 | 290 | 475 | 1.62e-19 | 90.5 |
| MS.gene010234.t1 | MTR_4g080800 | 38.318 | 107 | 65 | 1 | 6 | 111 | 5 | 111 | 1.02e-15 | 79.0 |
| MS.gene010234.t1 | MTR_2g083260 | 28.470 | 281 | 159 | 10 | 124 | 384 | 220 | 478 | 1.04e-18 | 87.8 |
| MS.gene010234.t1 | MTR_2g083260 | 37.273 | 110 | 68 | 1 | 3 | 111 | 2 | 111 | 3.15e-15 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010234.t1 | AT1G36050 | 82.124 | 386 | 69 | 0 | 1 | 386 | 1 | 386 | 0.0 | 699 |
| MS.gene010234.t1 | AT1G36050 | 81.937 | 382 | 69 | 0 | 1 | 382 | 1 | 382 | 0.0 | 692 |
| MS.gene010234.t1 | AT1G22200 | 77.461 | 386 | 87 | 0 | 1 | 386 | 1 | 386 | 0.0 | 654 |
| MS.gene010234.t1 | AT1G22200 | 76.418 | 335 | 79 | 0 | 1 | 335 | 1 | 335 | 0.0 | 563 |
| MS.gene010234.t1 | AT3G22290 | 36.031 | 383 | 202 | 10 | 4 | 379 | 3 | 349 | 3.42e-75 | 237 |
| MS.gene010234.t1 | AT3G22290 | 36.031 | 383 | 202 | 10 | 4 | 379 | 3 | 349 | 3.42e-75 | 237 |
| MS.gene010234.t1 | AT4G27080 | 31.841 | 201 | 104 | 7 | 202 | 384 | 293 | 478 | 1.73e-21 | 96.3 |
| MS.gene010234.t1 | AT4G27080 | 37.963 | 108 | 66 | 1 | 6 | 112 | 5 | 112 | 5.87e-16 | 79.7 |
| MS.gene010234.t1 | AT4G27080 | 31.841 | 201 | 104 | 7 | 202 | 384 | 345 | 530 | 3.08e-21 | 95.9 |
| MS.gene010234.t1 | AT4G27080 | 38.614 | 101 | 61 | 1 | 13 | 112 | 64 | 164 | 1.07e-13 | 72.8 |
| MS.gene010234.t1 | AT3G20560 | 30.693 | 202 | 108 | 7 | 202 | 384 | 293 | 481 | 4.99e-18 | 85.9 |
| MS.gene010234.t1 | AT3G20560 | 40.187 | 107 | 63 | 1 | 7 | 112 | 6 | 112 | 2.01e-17 | 84.0 |
| MS.gene010234.t1 | AT1G50950 | 26.690 | 281 | 167 | 9 | 124 | 384 | 221 | 482 | 6.76e-17 | 82.4 |
| MS.gene010234.t1 | AT1G50950 | 39.815 | 108 | 64 | 1 | 6 | 112 | 5 | 112 | 1.47e-16 | 81.6 |
| MS.gene010234.t1 | AT1G50950 | 26.690 | 281 | 167 | 9 | 124 | 384 | 226 | 487 | 6.99e-17 | 82.4 |
| MS.gene010234.t1 | AT1G50950 | 39.815 | 108 | 64 | 1 | 6 | 112 | 5 | 112 | 1.56e-16 | 81.6 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTATAGTCGCACTCTTTC+TGG | 0.133582 | 5.1:-48248988 | MS.gene010234:CDS |
| GCAGTTTGATGTTACATTTC+CGG | 0.239902 | 5.1:-48248053 | MS.gene010234:intron |
| ATTACGAAGCTTGTTCATTA+TGG | 0.253518 | 5.1:+48249041 | None:intergenic |
| CTACGACCTCTCTCCAATTA+AGG | 0.298777 | 5.1:-48239545 | MS.gene010234:intron |
| TCTTCTTCTTGATAGCCTTT+TGG | 0.307819 | 5.1:+48239087 | None:intergenic |
| GGCCGATTGCAGTCCCTCCC+TGG | 0.324323 | 5.1:-48239580 | MS.gene010234:CDS |
| TCTCTGTGACAGAACACTTT+AGG | 0.331930 | 5.1:-48239615 | MS.gene010234:CDS |
| CATCTGTATACCTCCAACTA+TGG | 0.342239 | 5.1:+48239273 | None:intergenic |
| GGCAAAAGCTTTCAGCAATC+TGG | 0.371884 | 5.1:-48244726 | MS.gene010234:CDS |
| TACCGCCTCAGCACCATAAC+AGG | 0.379587 | 5.1:+48247346 | None:intergenic |
| CTAGATTCATTTATCTATCA+TGG | 0.385708 | 5.1:-48239110 | MS.gene010234:CDS |
| GCCGATTGCAGTCCCTCCCT+GGG | 0.388413 | 5.1:-48239579 | MS.gene010234:CDS |
| CCCCAGGGAGGGACTGCAAT+CGG | 0.422047 | 5.1:+48239578 | None:intergenic |
| GAAACAAGGCAAGATGGAAT+TGG | 0.435169 | 5.1:-48247820 | MS.gene010234:CDS |
| GATCAATAACCTGGTTTGAC+TGG | 0.437886 | 5.1:+48239713 | None:intergenic |
| ACTTATTGCGGATCCTGTTA+TGG | 0.450203 | 5.1:-48247359 | MS.gene010234:CDS |
| TTGGAAGTAAACAAGGTGGC+AGG | 0.459413 | 5.1:-48244768 | MS.gene010234:CDS |
| CGTACATGTGCATGACCTGC+TGG | 0.468545 | 5.1:-48244703 | MS.gene010234:CDS |
| CTGCCTCCATGTCTTTGCAA+GGG | 0.472630 | 5.1:+48247398 | None:intergenic |
| GAAAAGCAAGAGCATGAGAA+TGG | 0.476070 | 5.1:+48248942 | None:intergenic |
| GAAAGAATCAAGGAAGAAGA+AGG | 0.480671 | 5.1:-48244816 | MS.gene010234:CDS |
| CACATCAACAGAATAGCCTT+TGG | 0.482453 | 5.1:-48242504 | MS.gene010234:CDS |
| CAAACCTGGTCAATTGCATC+CGG | 0.483871 | 5.1:+48245722 | None:intergenic |
| CCTGCCTCCATGTCTTTGCA+AGG | 0.483999 | 5.1:+48247397 | None:intergenic |
| CATTAGCGGAGAACAACATC+TGG | 0.491394 | 5.1:-48247993 | MS.gene010234:CDS |
| GAAAGAGAAATTACGAAGCT+CGG | 0.495749 | 5.1:+48248915 | None:intergenic |
| ACCAATGTGTGCGCCATAGT+TGG | 0.497118 | 5.1:-48239286 | MS.gene010234:CDS |
| CGTGAAGCATATCGAAAGAA+AGG | 0.502223 | 5.1:-48245761 | MS.gene010234:CDS |
| AAGCATATCGAAAGAAAGGT+TGG | 0.504184 | 5.1:-48245757 | MS.gene010234:CDS |
| ATTTATCTATCATGGCCAAA+AGG | 0.514131 | 5.1:-48239102 | MS.gene010234:CDS |
| GTGATAGAAACAAGGCAAGA+TGG | 0.530527 | 5.1:-48247826 | MS.gene010234:CDS |
| TCCAACTATGGCGCACACAT+TGG | 0.530726 | 5.1:+48239285 | None:intergenic |
| AAAGGCTATCAAGAAGAAGA+TGG | 0.538983 | 5.1:-48239084 | MS.gene010234:CDS |
| TTGTTCTCCGCTAATGTCCA+TGG | 0.540332 | 5.1:+48248000 | None:intergenic |
| CTCGATGCCATGGACATTAG+CGG | 0.540952 | 5.1:-48248007 | MS.gene010234:CDS |
| GTCAAGAGGGTTTACAACAC+CGG | 0.543744 | 5.1:+48242470 | None:intergenic |
| CTCACGTCAGTGTACATAGT+TGG | 0.544532 | 5.1:+48239748 | None:intergenic |
| GTCACTTAGTGCAAAAGAGA+AGG | 0.551191 | 5.1:-48244846 | MS.gene010234:intron |
| TCTTGATTATCAGAGTGCAT+TGG | 0.556387 | 5.1:-48242360 | MS.gene010234:intron |
| AGCATATCGAAAGAAAGGTT+GGG | 0.557532 | 5.1:-48245756 | MS.gene010234:CDS |
| CCTTGCAAAGACATGGAGGC+AGG | 0.557759 | 5.1:-48247397 | MS.gene010234:CDS |
| AGTATAGAACACGCTAGTGC+CGG | 0.558703 | 5.1:+48248034 | None:intergenic |
| TATACTTAGTCTCGATGCCA+TGG | 0.561660 | 5.1:-48248017 | MS.gene010234:CDS |
| GGTGAAGGATGCAATGTGTA+TGG | 0.569301 | 5.1:-48244795 | MS.gene010234:CDS |
| AAGAAAAGAATAGACTCTCA+TGG | 0.572185 | 5.1:-48247853 | MS.gene010234:CDS |
| GTGTTGTAAACCCTCTTGAC+CGG | 0.574958 | 5.1:-48242467 | MS.gene010234:intron |
| AGGTTGGGCACTCTCAAGCC+CGG | 0.575134 | 5.1:-48245741 | MS.gene010234:CDS |
| ATCAAGGAAGAAGAAGGTGA+AGG | 0.577027 | 5.1:-48244810 | MS.gene010234:CDS |
| TCACTTAGTGCAAAAGAGAA+GGG | 0.577545 | 5.1:-48244845 | MS.gene010234:intron |
| TAAAGAGCTCACCGGTCAAG+AGG | 0.585229 | 5.1:+48242456 | None:intergenic |
| AATGTGTGCGCCATAGTTGG+AGG | 0.586080 | 5.1:-48239283 | MS.gene010234:intron |
| TCAAGAGGGTTTACAACACC+GGG | 0.589163 | 5.1:+48242471 | None:intergenic |
| TGGAAGTAAACAAGGTGGCA+GGG | 0.593479 | 5.1:-48244767 | MS.gene010234:CDS |
| ATCAAGAAGAAGATGGAACT+CGG | 0.594892 | 5.1:-48239077 | MS.gene010234:CDS |
| TTTGCGCTTGCAGTCAGATG+AGG | 0.596325 | 5.1:-48245810 | MS.gene010234:intron |
| CTTGTTGTAGATACTTCTAG+AGG | 0.598128 | 5.1:-48248784 | MS.gene010234:CDS |
| GAGCACAATGAGACTTATTG+CGG | 0.606344 | 5.1:-48247371 | MS.gene010234:CDS |
| CTATGTACACTGACGTGAGT+GGG | 0.606656 | 5.1:-48239744 | MS.gene010234:CDS |
| AAGCCCGGATGCAATTGACC+AGG | 0.609558 | 5.1:-48245726 | MS.gene010234:intron |
| TGCTTCACGAACATCCTCAC+AGG | 0.609785 | 5.1:+48245775 | None:intergenic |
| AAACCTGGTCAATTGCATCC+GGG | 0.614091 | 5.1:+48245723 | None:intergenic |
| CAAAGGCTATTCTGTTGATG+TGG | 0.617708 | 5.1:+48242505 | None:intergenic |
| AAAGAGCTCACCGGTCAAGA+GGG | 0.621153 | 5.1:+48242457 | None:intergenic |
| ATCCTGTTATGGTGCTGAGG+CGG | 0.626803 | 5.1:-48247348 | MS.gene010234:intron |
| CACTTTAGGACAGCAGATGT+AGG | 0.632948 | 5.1:-48239601 | MS.gene010234:CDS |
| TATAGTCGCACTCTTTCTGG+CGG | 0.635746 | 5.1:-48248985 | MS.gene010234:CDS |
| GAACACTATCCAGTCAAACC+AGG | 0.636074 | 5.1:-48239722 | MS.gene010234:intron |
| ACCGGGAAAATAGTCTCCAA+AGG | 0.641478 | 5.1:+48242488 | None:intergenic |
| CGGATCCTGTTATGGTGCTG+AGG | 0.643851 | 5.1:-48247351 | MS.gene010234:CDS |
| AAAACACCTTAATTGGAGAG+AGG | 0.654012 | 5.1:+48239539 | None:intergenic |
| AAATACTGATACATCCCACT+AGG | 0.656800 | 5.1:+48242325 | None:intergenic |
| ACTATGTACACTGACGTGAG+TGG | 0.665706 | 5.1:-48239745 | MS.gene010234:CDS |
| CCGATTGCAGTCCCTCCCTG+GGG | 0.667693 | 5.1:-48239578 | MS.gene010234:CDS |
| GGACACAAGAAACACCTAGT+GGG | 0.680451 | 5.1:-48242339 | MS.gene010234:CDS |
| ATGGCAATGTGATAGAAACA+AGG | 0.687509 | 5.1:-48247834 | MS.gene010234:CDS |
| AAACCCTTGCAAAGACATGG+AGG | 0.692409 | 5.1:-48247401 | MS.gene010234:CDS |
| TGGACACAAGAAACACCTAG+TGG | 0.727967 | 5.1:-48242340 | MS.gene010234:CDS |
| GGAATGTTGTAATTCCTGTG+AGG | 0.801380 | 5.1:-48245789 | MS.gene010234:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 48239064 | 48249070 | 48239064 | ID=MS.gene010234 |
| chr5.1 | mRNA | 48239064 | 48249070 | 48239064 | ID=MS.gene010234.t1;Parent=MS.gene010234 |
| chr5.1 | exon | 48248929 | 48249070 | 48248929 | ID=MS.gene010234.t1.exon1;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48248929 | 48249070 | 48248929 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48248765 | 48248838 | 48248765 | ID=MS.gene010234.t1.exon2;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48248765 | 48248838 | 48248765 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48247988 | 48248071 | 48247988 | ID=MS.gene010234.t1.exon3;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48247988 | 48248071 | 48247988 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48247810 | 48247890 | 48247810 | ID=MS.gene010234.t1.exon4;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48247810 | 48247890 | 48247810 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48247349 | 48247429 | 48247349 | ID=MS.gene010234.t1.exon5;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48247349 | 48247429 | 48247349 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48245727 | 48245819 | 48245727 | ID=MS.gene010234.t1.exon6;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48245727 | 48245819 | 48245727 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48244680 | 48244859 | 48244680 | ID=MS.gene010234.t1.exon7;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48244680 | 48244859 | 48244680 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48242468 | 48242535 | 48242468 | ID=MS.gene010234.t1.exon8;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48242468 | 48242535 | 48242468 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48242318 | 48242369 | 48242318 | ID=MS.gene010234.t1.exon9;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48242318 | 48242369 | 48242318 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48239723 | 48239776 | 48239723 | ID=MS.gene010234.t1.exon10;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48239723 | 48239776 | 48239723 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48239546 | 48239638 | 48239546 | ID=MS.gene010234.t1.exon11;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48239546 | 48239638 | 48239546 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48239284 | 48239350 | 48239284 | ID=MS.gene010234.t1.exon12;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48239284 | 48239350 | 48239284 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| chr5.1 | exon | 48239064 | 48239155 | 48239064 | ID=MS.gene010234.t1.exon13;Parent=MS.gene010234.t1 |
| chr5.1 | CDS | 48239064 | 48239155 | 48239064 | ID=cds.MS.gene010234.t1;Parent=MS.gene010234.t1 |
| Gene Sequence |
| Protein sequence |
