AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012049


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012049.t1 MTR_8g076780 99.206 126 1 0 1 126 70 195 3.56e-81 236
MS.gene012049.t1 MTR_2g031960 53.043 115 52 1 14 126 41 155 1.41e-30 107
MS.gene012049.t1 MTR_2g031760 61.386 101 34 1 26 126 95 190 1.63e-29 105
MS.gene012049.t1 MTR_2g064300 51.304 115 47 2 20 126 103 216 7.90e-27 99.4
MS.gene012049.t1 MTR_7g053520 55.046 109 40 2 26 126 110 217 5.40e-26 97.4
MS.gene012049.t1 MTR_6g006060 45.714 70 38 0 56 125 111 180 7.83e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012049.t1 AT4G36420 61.111 126 42 3 1 126 61 179 9.15e-39 129
MS.gene012049.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012049.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012049.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012049.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene012049.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene012049.t1 AT4G37660 69.118 68 21 0 59 126 100 167 2.56e-24 91.7
MS.gene012049.t1 AT2G03130 48.315 89 42 1 38 126 7 91 2.52e-15 67.0
MS.gene012049.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene012049.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene012049.t1 AT3G27850 52.055 73 35 0 53 125 114 186 3.53e-15 68.6
MS.gene012049.t1 AT3G27840 43.210 81 46 0 45 125 112 192 1.64e-13 64.3

Find 0 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGTTATTAAAGAAGTT+AGG + 50848:17817-17836 MS.gene012049:CDS 15.0%
GATTATTGCTAAGTTAAAGG+AGG + 50848:17935-17954 MS.gene012049:CDS 30.0%
! AGTTAGGAGTTTTACTAGTT+TGG + 50848:17833-17852 MS.gene012049:CDS 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG + 50848:17834-17853 MS.gene012049:CDS 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG + 50848:17835-17854 MS.gene012049:CDS 30.0%
ATTGCTAAGTTAAAGGAGGT+TGG + 50848:17939-17958 MS.gene012049:CDS 35.0%
GAAGTAATGCGAGAGAAAAA+GGG + 50848:17615-17634 MS.gene012049:CDS 35.0%
GGAACCATCAACATCATAAT+CGG - 50848:17654-17673 MS.gene012049:intergenic 35.0%
GGGGATTATTGCTAAGTTAA+AGG + 50848:17932-17951 MS.gene012049:CDS 35.0%
TAACTTAGCAATAATCCCCT+CGG - 50848:17931-17950 MS.gene012049:intergenic 35.0%
TGAAGTAATGCGAGAGAAAA+AGG + 50848:17614-17633 MS.gene012049:CDS 35.0%
!! ATTATGATGTTGATGGTTCC+TGG + 50848:17654-17673 MS.gene012049:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG + 50848:17842-17861 MS.gene012049:CDS 35.0%
GCTTCCGATTATGATGTTGA+TGG + 50848:17647-17666 MS.gene012049:CDS 40.0%
GTTGAAGGAAGCTAAGGATT+TGG + 50848:17857-17876 MS.gene012049:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG - 50848:17894-17913 MS.gene012049:intergenic 40.0%
!! ATGTTGATGGTTCCTGGAAT+GGG + 50848:17660-17679 MS.gene012049:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG + 50848:17785-17804 MS.gene012049:CDS 40.0%
ATTCCCCTTATTCCCATTCC+AGG - 50848:17675-17694 MS.gene012049:intergenic 45.0%
GAAGGAAGCTAAGGATTTGG+TGG + 50848:17860-17879 MS.gene012049:CDS 45.0%
GCGTTTGATGTGAAGCTTGA+TGG + 50848:17774-17793 MS.gene012049:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG + 50848:17668-17687 MS.gene012049:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG + 50848:17669-17688 MS.gene012049:CDS 45.0%
TGGTTCCTGGAATGGGAATA+AGG + 50848:17667-17686 MS.gene012049:CDS 45.0%
! GAAAGGTGTGACGAAAGAAG+AGG + 50848:17905-17924 MS.gene012049:CDS 45.0%
! GGGGTGGTGATAGTGAAAAA+GGG + 50848:17721-17740 MS.gene012049:CDS 45.0%
! GGGTGGTGATAGTGAAAAAG+GGG + 50848:17722-17741 MS.gene012049:CDS 45.0%
! GGTGGTGATAGTGAAAAAGG+GGG + 50848:17723-17742 MS.gene012049:CDS 45.0%
! GGTTGGTGCAAAAGCTTCTA+TGG + 50848:17956-17975 MS.gene012049:CDS 45.0%
! TGATAGTGAAAAAGGGGGTG+AGG + 50848:17728-17747 MS.gene012049:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG + 50848:17851-17870 MS.gene012049:CDS 45.0%
!! GATGTTGATGGTTCCTGGAA+TGG + 50848:17659-17678 MS.gene012049:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG + 50848:17888-17907 MS.gene012049:CDS 45.0%
GGGTGAGGTGAAGAAAGTTG+AGG + 50848:17743-17762 MS.gene012049:CDS 50.0%
! GGGGGTGGTGATAGTGAAAA+AGG + 50848:17720-17739 MS.gene012049:CDS 50.0%
AGGTGTAGGTGGAGGGAAAG+GGG + 50848:17701-17720 MS.gene012049:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG + 50848:17700-17719 MS.gene012049:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG + 50848:17681-17700 MS.gene012049:CDS 55.0%
GTGACGAAAGAAGAGGCCGA+GGG + 50848:17912-17931 MS.gene012049:CDS 55.0%
TGACGAAAGAAGAGGCCGAG+GGG + 50848:17913-17932 MS.gene012049:CDS 55.0%
TGTGACGAAAGAAGAGGCCG+AGG + 50848:17911-17930 MS.gene012049:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG - 50848:17699-17718 MS.gene012049:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG + 50848:17699-17718 MS.gene012049:CDS 55.0%
GGTGTAGGTGGAGGGAAAGG+GGG + 50848:17702-17721 MS.gene012049:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG + 50848:17687-17706 MS.gene012049:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG + 50848:17693-17712 MS.gene012049:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG + 50848:17690-17709 MS.gene012049:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG + 50848:17694-17713 MS.gene012049:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG + 50848:17705-17724 MS.gene012049:CDS 65.0%


Chromosome Type Strat End Strand Name
50848 gene 17603 17983 17603 ID=MS.gene012049
50848 mRNA 17603 17983 17603 ID=MS.gene012049.t1;Parent=MS.gene012049
50848 exon 17603 17983 17603 ID=MS.gene012049.t1.exon1;Parent=MS.gene012049.t1
50848 CDS 17603 17983 17603 ID=cds.MS.gene012049.t1;Parent=MS.gene012049.t1
Gene Sequence

>MS.gene012049

ATGGATCTCGTTGAAGTAATGCGAGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGTTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGATAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCAAAGATTAAAGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAAGAAGAGGCCGAGGGGATTATTGCTAAGTTAAAGGAGGTTGGTGCAAAAGCTTCTATGGAATAA

Protein sequence

>MS.gene012049.t1

MDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGDSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME