AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012051


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012051.t1 MTR_8g076780 99.206 126 1 0 1 126 70 195 3.56e-81 236
MS.gene012051.t1 MTR_2g031960 53.043 115 52 1 14 126 41 155 1.41e-30 107
MS.gene012051.t1 MTR_2g031760 61.386 101 34 1 26 126 95 190 1.63e-29 105
MS.gene012051.t1 MTR_2g064300 51.304 115 47 2 20 126 103 216 7.90e-27 99.4
MS.gene012051.t1 MTR_7g053520 55.046 109 40 2 26 126 110 217 5.40e-26 97.4
MS.gene012051.t1 MTR_6g006060 45.714 70 38 0 56 125 111 180 7.83e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012051.t1 AT4G36420 61.111 126 42 3 1 126 61 179 9.15e-39 129
MS.gene012051.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012051.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012051.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012051.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene012051.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene012051.t1 AT4G37660 69.118 68 21 0 59 126 100 167 2.56e-24 91.7
MS.gene012051.t1 AT2G03130 48.315 89 42 1 38 126 7 91 2.52e-15 67.0
MS.gene012051.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene012051.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene012051.t1 AT3G27850 52.055 73 35 0 53 125 114 186 3.53e-15 68.6
MS.gene012051.t1 AT3G27840 43.210 81 46 0 45 125 112 192 1.64e-13 64.3

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTAATGCGAGAGAAAA+AGG 0.238846 8.1:-31154079 MS.gene012051:CDS
GGGGATTATTGCTAAGTTAA+AGG 0.252904 8.1:-31153761 MS.gene012051:CDS
TGGTTCCTGGAATGGGAATA+AGG 0.262636 8.1:-31154026 MS.gene012051:CDS
AGGTGTAGGTGGAGGGAAAG+GGG 0.337630 8.1:-31153992 MS.gene012051:CDS
TTAAAGTTATTAAAGAAGTT+AGG 0.349767 8.1:-31153876 MS.gene012051:CDS
GGTTGGTGCAAAAGCTTCTA+TGG 0.362512 8.1:-31153737 MS.gene012051:CDS
GGTTCCTGGAATGGGAATAA+GGG 0.368867 8.1:-31154025 MS.gene012051:CDS
GCGTTTGATGTGAAGCTTGA+TGG 0.371046 8.1:-31153919 MS.gene012051:CDS
ATTCCCCTTATTCCCATTCC+AGG 0.371516 8.1:+31154021 None:intergenic
GGTGTAGGTGGAGGGAAAGG+GGG 0.396694 8.1:-31153991 MS.gene012051:CDS
ATTATGATGTTGATGGTTCC+TGG 0.398854 8.1:-31154039 MS.gene012051:CDS
GCTTCCGATTATGATGTTGA+TGG 0.408175 8.1:-31154046 MS.gene012051:CDS
AGAGGTGTAGGTGGAGGGAA+AGG 0.431861 8.1:-31153994 MS.gene012051:CDS
GTTGAAGGAAGCTAAGGATT+TGG 0.435359 8.1:-31153836 MS.gene012051:CDS
GGAACCATCAACATCATAAT+CGG 0.459221 8.1:+31154042 None:intergenic
GAGGTGTAGGTGGAGGGAAA+GGG 0.479166 8.1:-31153993 MS.gene012051:CDS
ATGCCGAGAGGTGTAGGTGG+AGG 0.492930 8.1:-31154000 MS.gene012051:CDS
GATGTTGATGGTTCCTGGAA+TGG 0.504794 8.1:-31154034 MS.gene012051:CDS
ATTGCTAAGTTAAAGGAGGT+TGG 0.508274 8.1:-31153754 MS.gene012051:CDS
TTTGGGGTTGAAGGAAGCTA+AGG 0.515112 8.1:-31153842 MS.gene012051:CDS
TGTGACGAAAGAAGAGGCCG+AGG 0.536944 8.1:-31153782 MS.gene012051:CDS
ATGTTGATGGTTCCTGGAAT+GGG 0.541612 8.1:-31154033 MS.gene012051:CDS
AGGGGAATGCCGAGAGGTGT+AGG 0.541839 8.1:-31154006 MS.gene012051:CDS
GAAGGAAGCTAAGGATTTGG+TGG 0.548600 8.1:-31153833 MS.gene012051:CDS
TAACTTAGCAATAATCCCCT+CGG 0.571218 8.1:+31153765 None:intergenic
GGGTGAGGTGAAGAAAGTTG+AGG 0.571665 8.1:-31153950 MS.gene012051:CDS
TTCCCTCCACCTACACCTCT+CGG 0.576289 8.1:+31153997 None:intergenic
GATTATTGCTAAGTTAAAGG+AGG 0.580906 8.1:-31153758 MS.gene012051:CDS
TGCCGAGAGGTGTAGGTGGA+GGG 0.581642 8.1:-31153999 MS.gene012051:CDS
GGAATGCCGAGAGGTGTAGG+TGG 0.612707 8.1:-31154003 MS.gene012051:CDS
ACACCTTTCTTCAAAAGCGT+AGG 0.629486 8.1:+31153802 None:intergenic
GTGACGAAAGAAGAGGCCGA+GGG 0.636034 8.1:-31153781 MS.gene012051:CDS
GTTCCTGGAATGGGAATAAG+GGG 0.637463 8.1:-31154024 MS.gene012051:CDS
GAAAGGTGTGACGAAAGAAG+AGG 0.651585 8.1:-31153788 MS.gene012051:CDS
TGACGAAAGAAGAGGCCGAG+GGG 0.703464 8.1:-31153780 MS.gene012051:CDS
GGAATAAGGGGAATGCCGAG+AGG 0.729602 8.1:-31154012 MS.gene012051:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGTTATTAAAGAAGTT+AGG - chr8.1:31153946-31153965 MS.gene012051:CDS 15.0%
GATTATTGCTAAGTTAAAGG+AGG - chr8.1:31154064-31154083 MS.gene012051:CDS 30.0%
! AGTTAGGAGTTTTACTAGTT+TGG - chr8.1:31153962-31153981 MS.gene012051:CDS 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG - chr8.1:31153963-31153982 MS.gene012051:CDS 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG - chr8.1:31153964-31153983 MS.gene012051:CDS 30.0%
ATTGCTAAGTTAAAGGAGGT+TGG - chr8.1:31154068-31154087 MS.gene012051:CDS 35.0%
GAAGTAATGCGAGAGAAAAA+GGG - chr8.1:31153744-31153763 MS.gene012051:CDS 35.0%
GGAACCATCAACATCATAAT+CGG + chr8.1:31153783-31153802 None:intergenic 35.0%
GGGGATTATTGCTAAGTTAA+AGG - chr8.1:31154061-31154080 MS.gene012051:CDS 35.0%
TAACTTAGCAATAATCCCCT+CGG + chr8.1:31154060-31154079 None:intergenic 35.0%
TGAAGTAATGCGAGAGAAAA+AGG - chr8.1:31153743-31153762 MS.gene012051:CDS 35.0%
!! ATTATGATGTTGATGGTTCC+TGG - chr8.1:31153783-31153802 MS.gene012051:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG - chr8.1:31153971-31153990 MS.gene012051:CDS 35.0%
GCTTCCGATTATGATGTTGA+TGG - chr8.1:31153776-31153795 MS.gene012051:CDS 40.0%
GTTGAAGGAAGCTAAGGATT+TGG - chr8.1:31153986-31154005 MS.gene012051:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG + chr8.1:31154023-31154042 None:intergenic 40.0%
!! ATGTTGATGGTTCCTGGAAT+GGG - chr8.1:31153789-31153808 MS.gene012051:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG - chr8.1:31153914-31153933 MS.gene012051:CDS 40.0%
ATTCCCCTTATTCCCATTCC+AGG + chr8.1:31153804-31153823 None:intergenic 45.0%
GAAGGAAGCTAAGGATTTGG+TGG - chr8.1:31153989-31154008 MS.gene012051:CDS 45.0%
GCGTTTGATGTGAAGCTTGA+TGG - chr8.1:31153903-31153922 MS.gene012051:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG - chr8.1:31153797-31153816 MS.gene012051:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG - chr8.1:31153798-31153817 MS.gene012051:CDS 45.0%
TGGTTCCTGGAATGGGAATA+AGG - chr8.1:31153796-31153815 MS.gene012051:CDS 45.0%
! GAAAGGTGTGACGAAAGAAG+AGG - chr8.1:31154034-31154053 MS.gene012051:CDS 45.0%
! GGGGTGGTGATAGTGAAAAA+GGG - chr8.1:31153850-31153869 MS.gene012051:CDS 45.0%
! GGGTGGTGATAGTGAAAAAG+GGG - chr8.1:31153851-31153870 MS.gene012051:CDS 45.0%
! GGTGGTGATAGTGAAAAAGG+GGG - chr8.1:31153852-31153871 MS.gene012051:CDS 45.0%
! GGTTGGTGCAAAAGCTTCTA+TGG - chr8.1:31154085-31154104 MS.gene012051:CDS 45.0%
! TGATAGTGAAAAAGGGGGTG+AGG - chr8.1:31153857-31153876 MS.gene012051:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG - chr8.1:31153980-31153999 MS.gene012051:CDS 45.0%
!! GATGTTGATGGTTCCTGGAA+TGG - chr8.1:31153788-31153807 MS.gene012051:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG - chr8.1:31154017-31154036 MS.gene012051:CDS 45.0%
GGGTGAGGTGAAGAAAGTTG+AGG - chr8.1:31153872-31153891 MS.gene012051:CDS 50.0%
! GGGGGTGGTGATAGTGAAAA+AGG - chr8.1:31153849-31153868 MS.gene012051:CDS 50.0%
AGGTGTAGGTGGAGGGAAAG+GGG - chr8.1:31153830-31153849 MS.gene012051:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG - chr8.1:31153829-31153848 MS.gene012051:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG - chr8.1:31153810-31153829 MS.gene012051:CDS 55.0%
GTGACGAAAGAAGAGGCCGA+GGG - chr8.1:31154041-31154060 MS.gene012051:CDS 55.0%
TGACGAAAGAAGAGGCCGAG+GGG - chr8.1:31154042-31154061 MS.gene012051:CDS 55.0%
TGTGACGAAAGAAGAGGCCG+AGG - chr8.1:31154040-31154059 MS.gene012051:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG + chr8.1:31153828-31153847 None:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG - chr8.1:31153828-31153847 MS.gene012051:CDS 55.0%
GGTGTAGGTGGAGGGAAAGG+GGG - chr8.1:31153831-31153850 MS.gene012051:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG - chr8.1:31153816-31153835 MS.gene012051:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG - chr8.1:31153822-31153841 MS.gene012051:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG - chr8.1:31153819-31153838 MS.gene012051:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG - chr8.1:31153823-31153842 MS.gene012051:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG - chr8.1:31153834-31153853 MS.gene012051:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31153732 31154112 31153732 ID=MS.gene012051
chr8.1 mRNA 31153732 31154112 31153732 ID=MS.gene012051.t1;Parent=MS.gene012051
chr8.1 exon 31153732 31154112 31153732 ID=MS.gene012051.t1.exon1;Parent=MS.gene012051.t1
chr8.1 CDS 31153732 31154112 31153732 ID=cds.MS.gene012051.t1;Parent=MS.gene012051.t1
Gene Sequence

>MS.gene012051

ATGGATCTCGTTGAAGTAATGCGAGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGTTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGATAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCAAAGATTAAAGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAAGAAGAGGCCGAGGGGATTATTGCTAAGTTAAAGGAGGTTGGTGCAAAAGCTTCTATGGAATAA

Protein sequence

>MS.gene012051.t1

MDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGDSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME