AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012053


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012053.t1 MTR_8g076780 99.206 126 1 0 1 126 70 195 3.56e-81 236
MS.gene012053.t1 MTR_2g031960 53.043 115 52 1 14 126 41 155 1.41e-30 107
MS.gene012053.t1 MTR_2g031760 61.386 101 34 1 26 126 95 190 1.63e-29 105
MS.gene012053.t1 MTR_2g064300 51.304 115 47 2 20 126 103 216 7.90e-27 99.4
MS.gene012053.t1 MTR_7g053520 55.046 109 40 2 26 126 110 217 5.40e-26 97.4
MS.gene012053.t1 MTR_6g006060 45.714 70 38 0 56 125 111 180 7.83e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012053.t1 AT4G36420 61.111 126 42 3 1 126 61 179 9.15e-39 129
MS.gene012053.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012053.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012053.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene012053.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene012053.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene012053.t1 AT4G37660 69.118 68 21 0 59 126 100 167 2.56e-24 91.7
MS.gene012053.t1 AT2G03130 48.315 89 42 1 38 126 7 91 2.52e-15 67.0
MS.gene012053.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene012053.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene012053.t1 AT3G27850 52.055 73 35 0 53 125 114 186 3.53e-15 68.6
MS.gene012053.t1 AT3G27840 43.210 81 46 0 45 125 112 192 1.64e-13 64.3

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTAATGCGAGAGAAAA+AGG 0.238846 8.1:-31079618 MS.gene012053:CDS
GGGGATTATTGCTAAGTTAA+AGG 0.252904 8.1:-31079300 MS.gene012053:CDS
TGGTTCCTGGAATGGGAATA+AGG 0.262636 8.1:-31079565 MS.gene012053:CDS
AGGTGTAGGTGGAGGGAAAG+GGG 0.337630 8.1:-31079531 MS.gene012053:CDS
TTAAAGTTATTAAAGAAGTT+AGG 0.349767 8.1:-31079415 MS.gene012053:CDS
GGTTGGTGCAAAAGCTTCTA+TGG 0.362512 8.1:-31079276 MS.gene012053:CDS
GGTTCCTGGAATGGGAATAA+GGG 0.368867 8.1:-31079564 MS.gene012053:CDS
GCGTTTGATGTGAAGCTTGA+TGG 0.371046 8.1:-31079458 MS.gene012053:CDS
ATTCCCCTTATTCCCATTCC+AGG 0.371516 8.1:+31079560 None:intergenic
GGTGTAGGTGGAGGGAAAGG+GGG 0.396694 8.1:-31079530 MS.gene012053:CDS
ATTATGATGTTGATGGTTCC+TGG 0.398854 8.1:-31079578 MS.gene012053:CDS
GCTTCCGATTATGATGTTGA+TGG 0.408175 8.1:-31079585 MS.gene012053:CDS
AGAGGTGTAGGTGGAGGGAA+AGG 0.431861 8.1:-31079533 MS.gene012053:CDS
GTTGAAGGAAGCTAAGGATT+TGG 0.435359 8.1:-31079375 MS.gene012053:CDS
GGAACCATCAACATCATAAT+CGG 0.459221 8.1:+31079581 None:intergenic
GAGGTGTAGGTGGAGGGAAA+GGG 0.479166 8.1:-31079532 MS.gene012053:CDS
ATGCCGAGAGGTGTAGGTGG+AGG 0.492930 8.1:-31079539 MS.gene012053:CDS
GATGTTGATGGTTCCTGGAA+TGG 0.504794 8.1:-31079573 MS.gene012053:CDS
ATTGCTAAGTTAAAGGAGGT+TGG 0.508274 8.1:-31079293 MS.gene012053:CDS
TTTGGGGTTGAAGGAAGCTA+AGG 0.515112 8.1:-31079381 MS.gene012053:CDS
TGTGACGAAAGAAGAGGCCG+AGG 0.536944 8.1:-31079321 MS.gene012053:CDS
ATGTTGATGGTTCCTGGAAT+GGG 0.541612 8.1:-31079572 MS.gene012053:CDS
AGGGGAATGCCGAGAGGTGT+AGG 0.541839 8.1:-31079545 MS.gene012053:CDS
GAAGGAAGCTAAGGATTTGG+TGG 0.548600 8.1:-31079372 MS.gene012053:CDS
TAACTTAGCAATAATCCCCT+CGG 0.571218 8.1:+31079304 None:intergenic
GGGTGAGGTGAAGAAAGTTG+AGG 0.571665 8.1:-31079489 MS.gene012053:CDS
TTCCCTCCACCTACACCTCT+CGG 0.576289 8.1:+31079536 None:intergenic
GATTATTGCTAAGTTAAAGG+AGG 0.580906 8.1:-31079297 MS.gene012053:CDS
TGCCGAGAGGTGTAGGTGGA+GGG 0.581642 8.1:-31079538 MS.gene012053:CDS
GGAATGCCGAGAGGTGTAGG+TGG 0.612707 8.1:-31079542 MS.gene012053:CDS
ACACCTTTCTTCAAAAGCGT+AGG 0.629486 8.1:+31079341 None:intergenic
GTGACGAAAGAAGAGGCCGA+GGG 0.636034 8.1:-31079320 MS.gene012053:CDS
GTTCCTGGAATGGGAATAAG+GGG 0.637463 8.1:-31079563 MS.gene012053:CDS
GAAAGGTGTGACGAAAGAAG+AGG 0.651585 8.1:-31079327 MS.gene012053:CDS
TGACGAAAGAAGAGGCCGAG+GGG 0.703464 8.1:-31079319 MS.gene012053:CDS
GGAATAAGGGGAATGCCGAG+AGG 0.729602 8.1:-31079551 MS.gene012053:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGTTATTAAAGAAGTT+AGG - chr8.1:31079485-31079504 MS.gene012053:CDS 15.0%
GATTATTGCTAAGTTAAAGG+AGG - chr8.1:31079603-31079622 MS.gene012053:CDS 30.0%
! AGTTAGGAGTTTTACTAGTT+TGG - chr8.1:31079501-31079520 MS.gene012053:CDS 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG - chr8.1:31079502-31079521 MS.gene012053:CDS 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG - chr8.1:31079503-31079522 MS.gene012053:CDS 30.0%
ATTGCTAAGTTAAAGGAGGT+TGG - chr8.1:31079607-31079626 MS.gene012053:CDS 35.0%
GAAGTAATGCGAGAGAAAAA+GGG - chr8.1:31079283-31079302 MS.gene012053:CDS 35.0%
GGAACCATCAACATCATAAT+CGG + chr8.1:31079322-31079341 None:intergenic 35.0%
GGGGATTATTGCTAAGTTAA+AGG - chr8.1:31079600-31079619 MS.gene012053:CDS 35.0%
TAACTTAGCAATAATCCCCT+CGG + chr8.1:31079599-31079618 None:intergenic 35.0%
TGAAGTAATGCGAGAGAAAA+AGG - chr8.1:31079282-31079301 MS.gene012053:CDS 35.0%
!! ATTATGATGTTGATGGTTCC+TGG - chr8.1:31079322-31079341 MS.gene012053:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG - chr8.1:31079510-31079529 MS.gene012053:CDS 35.0%
GCTTCCGATTATGATGTTGA+TGG - chr8.1:31079315-31079334 MS.gene012053:CDS 40.0%
GTTGAAGGAAGCTAAGGATT+TGG - chr8.1:31079525-31079544 MS.gene012053:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG + chr8.1:31079562-31079581 None:intergenic 40.0%
!! ATGTTGATGGTTCCTGGAAT+GGG - chr8.1:31079328-31079347 MS.gene012053:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG - chr8.1:31079453-31079472 MS.gene012053:CDS 40.0%
ATTCCCCTTATTCCCATTCC+AGG + chr8.1:31079343-31079362 None:intergenic 45.0%
GAAGGAAGCTAAGGATTTGG+TGG - chr8.1:31079528-31079547 MS.gene012053:CDS 45.0%
GCGTTTGATGTGAAGCTTGA+TGG - chr8.1:31079442-31079461 MS.gene012053:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG - chr8.1:31079336-31079355 MS.gene012053:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG - chr8.1:31079337-31079356 MS.gene012053:CDS 45.0%
TGGTTCCTGGAATGGGAATA+AGG - chr8.1:31079335-31079354 MS.gene012053:CDS 45.0%
! GAAAGGTGTGACGAAAGAAG+AGG - chr8.1:31079573-31079592 MS.gene012053:CDS 45.0%
! GGGGTGGTGATAGTGAAAAA+GGG - chr8.1:31079389-31079408 MS.gene012053:CDS 45.0%
! GGGTGGTGATAGTGAAAAAG+GGG - chr8.1:31079390-31079409 MS.gene012053:CDS 45.0%
! GGTGGTGATAGTGAAAAAGG+GGG - chr8.1:31079391-31079410 MS.gene012053:CDS 45.0%
! GGTTGGTGCAAAAGCTTCTA+TGG - chr8.1:31079624-31079643 MS.gene012053:CDS 45.0%
! TGATAGTGAAAAAGGGGGTG+AGG - chr8.1:31079396-31079415 MS.gene012053:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG - chr8.1:31079519-31079538 MS.gene012053:CDS 45.0%
!! GATGTTGATGGTTCCTGGAA+TGG - chr8.1:31079327-31079346 MS.gene012053:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG - chr8.1:31079556-31079575 MS.gene012053:CDS 45.0%
GGGTGAGGTGAAGAAAGTTG+AGG - chr8.1:31079411-31079430 MS.gene012053:CDS 50.0%
! GGGGGTGGTGATAGTGAAAA+AGG - chr8.1:31079388-31079407 MS.gene012053:CDS 50.0%
AGGTGTAGGTGGAGGGAAAG+GGG - chr8.1:31079369-31079388 MS.gene012053:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG - chr8.1:31079368-31079387 MS.gene012053:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG - chr8.1:31079349-31079368 MS.gene012053:CDS 55.0%
GTGACGAAAGAAGAGGCCGA+GGG - chr8.1:31079580-31079599 MS.gene012053:CDS 55.0%
TGACGAAAGAAGAGGCCGAG+GGG - chr8.1:31079581-31079600 MS.gene012053:CDS 55.0%
TGTGACGAAAGAAGAGGCCG+AGG - chr8.1:31079579-31079598 MS.gene012053:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG + chr8.1:31079367-31079386 None:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG - chr8.1:31079367-31079386 MS.gene012053:CDS 55.0%
GGTGTAGGTGGAGGGAAAGG+GGG - chr8.1:31079370-31079389 MS.gene012053:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG - chr8.1:31079355-31079374 MS.gene012053:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG - chr8.1:31079361-31079380 MS.gene012053:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG - chr8.1:31079358-31079377 MS.gene012053:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG - chr8.1:31079362-31079381 MS.gene012053:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG - chr8.1:31079373-31079392 MS.gene012053:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31079271 31079651 31079271 ID=MS.gene012053
chr8.1 mRNA 31079271 31079651 31079271 ID=MS.gene012053.t1;Parent=MS.gene012053
chr8.1 exon 31079271 31079651 31079271 ID=MS.gene012053.t1.exon1;Parent=MS.gene012053.t1
chr8.1 CDS 31079271 31079651 31079271 ID=cds.MS.gene012053.t1;Parent=MS.gene012053.t1
Gene Sequence

>MS.gene012053

ATGGATCTCGTTGAAGTAATGCGAGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGTTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGATAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCAAAGATTAAAGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAAGAAGAGGCCGAGGGGATTATTGCTAAGTTAAAGGAGGTTGGTGCAAAAGCTTCTATGGAATAA

Protein sequence

>MS.gene012053.t1

MDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGDSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME