AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01262

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01262.t1 MTR_2g010450 94.901 353 18 0 1 353 1 353 0.0 696
MS.gene01262.t1 MTR_4g092700 55.301 349 112 10 7 353 27 333 2.07e-114 335
MS.gene01262.t1 MTR_4g097940 40.230 348 172 14 5 345 2 320 4.66e-59 194
MS.gene01262.t1 MTR_4g097920 40.230 348 172 14 5 345 2 320 3.37e-58 191
MS.gene01262.t1 MTR_5g014600 44.358 257 115 6 106 346 86 330 2.61e-57 189
MS.gene01262.t1 MTR_5g014520 52.632 190 87 2 166 353 144 332 6.47e-55 183
MS.gene01262.t1 MTR_4g097920 42.244 303 148 12 44 345 29 305 1.84e-53 179
MS.gene01262.t1 MTR_0250s0040 45.813 203 106 2 151 353 65 263 1.00e-50 170
MS.gene01262.t1 MTR_0246s0020 45.089 224 106 6 138 353 163 377 3.41e-49 169
MS.gene01262.t1 MTR_5g014640 34.104 346 200 10 5 348 2 321 6.57e-49 167
MS.gene01262.t1 MTR_4g097950 54.749 179 69 4 177 346 70 245 1.61e-48 164
MS.gene01262.t1 MTR_8g009020 42.081 221 116 4 134 346 102 318 2.73e-48 166
MS.gene01262.t1 MTR_6g047570 46.154 195 100 4 161 353 143 334 3.70e-47 163
MS.gene01262.t1 MTR_5g014560 42.793 222 117 4 131 348 86 301 1.76e-46 160
MS.gene01262.t1 MTR_2g104550 50.847 177 81 2 173 346 134 307 2.79e-46 160
MS.gene01262.t1 MTR_0011s0210 47.059 187 82 4 175 353 181 358 8.87e-46 160
MS.gene01262.t1 MTR_0011s0260 45.810 179 96 1 175 353 181 358 2.62e-45 159
MS.gene01262.t1 MTR_0246s0020 45.771 201 92 5 138 330 163 354 6.26e-44 155
MS.gene01262.t1 MTR_4g067010 43.548 186 99 2 168 353 140 319 6.41e-43 152
MS.gene01262.t1 MTR_4g098035 39.904 208 107 3 156 353 141 340 1.67e-41 148
MS.gene01262.t1 MTR_0246s0050 39.904 208 107 3 156 353 120 319 2.11e-41 147
MS.gene01262.t1 MTR_4g009540 45.161 186 93 3 168 346 143 326 1.77e-39 142
MS.gene01262.t1 MTR_4g009110 46.067 178 90 2 173 346 170 345 2.17e-39 143
MS.gene01262.t1 MTR_2g104490 46.927 179 92 2 173 351 133 308 2.18e-39 142
MS.gene01262.t1 MTR_4g066460 41.627 209 111 3 151 352 122 326 7.09e-39 141
MS.gene01262.t1 MTR_2g104560 42.697 178 89 5 174 346 51 220 8.45e-39 138
MS.gene01262.t1 MTR_4g066380 40.952 210 112 3 151 353 120 324 1.66e-37 137
MS.gene01262.t1 MTR_2g104490 46.386 166 86 2 173 338 133 295 1.96e-36 134
MS.gene01262.t1 MTR_2g104650 43.094 181 100 2 168 346 85 264 8.98e-36 131
MS.gene01262.t1 MTR_8g009010 40.230 174 66 2 174 346 131 267 2.67e-35 131
MS.gene01262.t1 MTR_4g009540 45.294 170 84 3 168 330 143 310 1.16e-34 129
MS.gene01262.t1 MTR_8g069740 43.636 165 88 4 168 329 103 265 6.52e-34 126
MS.gene01262.t1 MTR_2g104590 39.773 176 83 3 173 346 65 219 2.88e-32 121
MS.gene01262.t1 MTR_7g080780 41.860 172 90 1 175 346 150 311 3.95e-31 120
MS.gene01262.t1 MTR_7g080780 41.860 172 90 1 175 346 163 324 5.95e-31 120
MS.gene01262.t1 MTR_2g104530 38.150 173 94 3 178 346 106 269 1.11e-29 115
MS.gene01262.t1 MTR_2g104500 38.728 173 94 4 177 346 104 267 4.34e-29 113
MS.gene01262.t1 MTR_7g451650 38.816 152 82 2 200 351 57 197 1.44e-25 102
MS.gene01262.t1 MTR_4g009510 35.260 173 99 4 180 351 45 205 4.28e-24 98.6
MS.gene01262.t1 MTR_7g050350 37.671 146 80 2 206 351 1 135 7.28e-24 95.9
MS.gene01262.t1 MTR_7g451650 37.671 146 80 2 206 351 1 135 7.28e-24 95.9
MS.gene01262.t1 MTR_4g009420 37.500 152 76 2 200 351 65 197 2.45e-23 96.3
MS.gene01262.t1 MTR_5g014550 41.732 127 73 1 207 333 54 179 1.28e-22 94.4
MS.gene01262.t1 MTR_6g047550 44.262 122 62 3 130 249 127 244 7.06e-22 94.0
MS.gene01262.t1 MTR_7g451650 37.778 135 73 2 200 334 57 180 2.53e-21 90.5
MS.gene01262.t1 MTR_1g072320 34.395 157 93 3 177 324 470 625 7.85e-20 91.3
MS.gene01262.t1 MTR_4g009420 36.296 135 67 2 200 334 65 180 5.82e-19 84.3
MS.gene01262.t1 MTR_8g027495 32.353 136 83 2 177 312 458 584 3.65e-16 80.1
MS.gene01262.t1 MTR_7g117670 31.618 136 84 2 177 312 402 528 5.16e-16 79.7
MS.gene01262.t1 MTR_3g104100 29.032 155 101 2 179 324 197 351 1.53e-15 77.4
MS.gene01262.t1 MTR_8g067280 33.143 175 106 3 150 313 434 608 1.68e-15 78.2
MS.gene01262.t1 MTR_5g030430 31.333 150 87 3 177 313 493 639 5.69e-15 76.6
MS.gene01262.t1 MTR_3g030700 29.189 185 120 4 149 329 175 352 1.04e-14 75.1
MS.gene01262.t1 MTR_8g024790 32.168 143 85 3 177 313 291 427 1.12e-14 75.1
MS.gene01262.t1 MTR_3g498695 29.032 155 101 2 179 324 300 454 1.26e-14 75.1
MS.gene01262.t1 MTR_3g498695 29.032 155 101 2 179 324 300 454 1.26e-14 75.1
MS.gene01262.t1 MTR_7g096350 54.386 57 26 0 177 233 355 411 8.77e-14 72.8
MS.gene01262.t1 MTR_8g024770 27.711 166 99 3 177 330 279 435 1.80e-13 71.6
MS.gene01262.t1 MTR_7g083900 28.302 159 99 3 179 324 288 444 3.16e-13 70.9
MS.gene01262.t1 MTR_7g083900 28.302 159 99 3 179 324 288 444 3.35e-13 70.9
MS.gene01262.t1 MTR_2g081040 29.032 155 101 3 179 324 179 333 1.10e-12 68.9
MS.gene01262.t1 MTR_8g064700 25.843 178 110 5 166 327 227 398 1.42e-11 65.5
MS.gene01262.t1 MTR_8g098275 25.962 208 121 5 174 352 241 444 3.56e-11 64.3
MS.gene01262.t1 MTR_8g098275 25.962 208 121 5 174 352 305 508 5.80e-11 63.9
MS.gene01262.t1 MTR_1g112680 38.710 93 53 2 168 257 309 400 6.64e-11 63.9
MS.gene01262.t1 MTR_8g098275 25.962 208 121 5 174 352 398 601 6.73e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01262.t1 AT4G37850 56.757 185 72 4 173 353 148 328 9.90e-60 196
MS.gene01262.t1 AT4G37850 56.757 185 72 4 173 353 148 328 9.90e-60 196
MS.gene01262.t1 AT2G22760 49.153 177 87 3 175 348 117 293 1.10e-48 166
MS.gene01262.t1 AT2G22770 46.809 188 86 2 177 350 132 319 7.09e-45 156
MS.gene01262.t1 AT2G22750 50.811 185 84 3 173 352 122 304 3.38e-43 152
MS.gene01262.t1 AT2G22750 50.811 185 84 3 173 352 146 328 7.15e-43 151
MS.gene01262.t1 AT2G22750 50.811 185 84 3 173 352 150 332 7.37e-43 152
MS.gene01262.t1 AT2G22750 50.811 185 83 4 173 352 122 303 7.11e-42 148
MS.gene01262.t1 AT2G22760 47.468 158 81 2 175 330 117 274 1.29e-41 147
MS.gene01262.t1 AT2G22750 50.811 185 83 4 173 352 150 331 1.63e-41 148
MS.gene01262.t1 AT1G32640 36.025 161 87 4 165 313 432 588 1.18e-18 87.8
MS.gene01262.t1 AT5G46760 34.783 161 101 2 177 334 415 574 3.89e-17 83.2
MS.gene01262.t1 AT2G46510 59.649 57 23 0 177 233 395 451 1.20e-16 81.6
MS.gene01262.t1 AT4G16430 37.143 140 70 5 118 241 247 384 3.30e-16 80.1
MS.gene01262.t1 AT1G01260 52.174 69 32 1 168 235 423 491 8.63e-16 79.0
MS.gene01262.t1 AT1G01260 52.174 69 32 1 168 235 423 491 8.63e-16 79.0
MS.gene01262.t1 AT1G01260 52.174 69 32 1 168 235 423 491 8.63e-16 79.0
MS.gene01262.t1 AT4G17880 31.013 158 107 2 177 334 416 571 1.36e-15 78.2
MS.gene01262.t1 AT1G12860 30.921 152 100 3 178 324 268 419 2.07e-14 74.3
MS.gene01262.t1 AT1G12860 31.126 151 99 3 179 324 269 419 2.35e-14 74.3
MS.gene01262.t1 AT3G26744 30.128 156 100 3 178 324 308 463 2.69e-13 71.2
MS.gene01262.t1 AT3G26744 30.128 156 100 3 178 324 308 463 3.05e-13 70.9
MS.gene01262.t1 AT3G26744 30.128 156 100 3 178 324 308 463 3.05e-13 70.9
MS.gene01262.t1 AT3G26744 30.128 156 100 3 178 324 308 463 3.05e-13 70.9
MS.gene01262.t1 AT5G57150 30.000 170 97 5 173 327 51 213 1.04e-12 67.0
MS.gene01262.t1 AT5G57150 30.000 170 97 5 173 327 51 213 1.22e-12 67.0
MS.gene01262.t1 AT5G57150 30.000 170 97 5 173 327 50 212 1.33e-12 67.0
MS.gene01262.t1 AT4G09820 31.343 134 65 3 178 308 364 473 1.46e-12 68.9
MS.gene01262.t1 AT5G57150 30.000 170 97 5 173 327 51 213 1.68e-12 67.4
MS.gene01262.t1 AT2G16910 28.049 164 89 4 179 317 316 475 2.05e-12 68.6
MS.gene01262.t1 AT5G57150 30.000 170 97 5 173 327 50 212 2.21e-12 66.6
MS.gene01262.t1 AT5G41315 28.824 170 99 3 160 307 429 598 2.28e-12 68.6
MS.gene01262.t1 AT5G41315 28.824 170 99 3 160 307 424 593 2.44e-12 68.6
MS.gene01262.t1 AT5G41315 28.824 170 99 3 160 307 432 601 2.47e-12 68.6
MS.gene01262.t1 AT3G61950 30.496 141 87 3 177 311 128 263 4.73e-12 66.2
MS.gene01262.t1 AT4G00870 46.875 64 34 0 178 241 250 313 5.87e-12 67.0
MS.gene01262.t1 AT3G61950 30.496 141 87 3 177 311 179 314 8.80e-12 65.9
MS.gene01262.t1 AT3G61950 30.496 141 87 3 177 311 128 263 9.53e-12 65.5
MS.gene01262.t1 AT3G61950 30.496 141 87 3 177 311 179 314 1.79e-11 65.1
MS.gene01262.t1 AT1G63650 27.564 156 96 3 169 307 397 552 1.89e-11 65.9
MS.gene01262.t1 AT1G63650 27.564 156 96 3 169 307 397 552 1.89e-11 65.9
MS.gene01262.t1 AT1G63650 27.564 156 96 3 169 307 397 552 1.89e-11 65.9
MS.gene01262.t1 AT4G29930 27.778 180 107 6 178 349 55 219 2.05e-11 63.5
MS.gene01262.t1 AT4G29930 28.161 174 111 4 178 349 55 216 3.17e-11 62.8
MS.gene01262.t1 AT4G29930 25.773 194 119 3 178 349 55 245 5.52e-11 62.8

Find 54 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTTGCTTCAATTTC+AGG 0.082646 2.2:+71041542 None:intergenic
AATATATATCTTACCATTTC+AGG 0.177016 2.2:+71043070 None:intergenic
AACTTAACTGCTTCTGCTTT+TGG 0.208058 2.2:-71042750 MS.gene01262:CDS
AGCAGAAGCAGTTAAGTTAT+TGG 0.221213 2.2:+71042755 None:intergenic
ATGGACAAAGCTTCTGTTCT+TGG 0.230457 2.2:-71041737 MS.gene01262:CDS
AATAGCAGTTCCTTGACTTT+TGG 0.241912 2.2:-71041422 MS.gene01262:CDS
TTTCACCGGTCTTTCGTTTC+CGG 0.252447 2.2:+71042653 None:intergenic
GTTGTTGAAGTTTGGATTAT+TGG 0.283921 2.2:+71042701 None:intergenic
ATCACTCAAGGTAACTTGTT+TGG 0.298879 2.2:-71042438 MS.gene01262:CDS
ATGATTTCTGCAGGGGATAA+TGG 0.322117 2.2:-71042829 MS.gene01262:intron
TTTGATACCTTCTTTAGGTT+TGG 0.325711 2.2:+71042264 None:intergenic
CATTATTTGATACCTTCTTT+AGG 0.327577 2.2:+71042259 None:intergenic
TCACTCAAGGTAACTTGTTT+GGG 0.334384 2.2:-71042437 MS.gene01262:CDS
TTAAAGTGATGGAGAAAGTT+AGG 0.337641 2.2:+71042801 None:intergenic
CTGTTCTTGGAGAAGCTATA+AGG 0.338206 2.2:-71041724 MS.gene01262:CDS
TATTGGTATCAATAGAGTTT+AGG 0.363520 2.2:+71042772 None:intergenic
TATCAGAGGGTTACCTGAAA+TGG 0.381568 2.2:-71043083 MS.gene01262:intron
TTCCAGGATGAGTTCTCATA+TGG 0.444780 2.2:-71043116 None:intergenic
GAAAATTACCTGAGCAATGA+TGG 0.451657 2.2:+71041379 None:intergenic
TTGAATTACACAAATCAGTT+GGG 0.457187 2.2:-71042531 MS.gene01262:CDS
TCAAACAATGTAGTCTTATG+AGG 0.459967 2.2:+71041507 None:intergenic
TATAAGGTACTTAAAGCAAA+TGG 0.467859 2.2:-71041708 MS.gene01262:CDS
GAGCAATGATGGTTATATCA+AGG 0.480152 2.2:+71041390 None:intergenic
TTAGGTGATTATTAAAGTGA+TGG 0.488258 2.2:+71042790 None:intergenic
TTTGAATTACACAAATCAGT+TGG 0.490344 2.2:-71042532 MS.gene01262:CDS
TTATTTCTTAACTGTTTCAC+CGG 0.499185 2.2:+71042639 None:intergenic
AACAGTTAAGAAATAACAGC+TGG 0.500916 2.2:-71042632 MS.gene01262:CDS
TGATATAACCATCATTGCTC+AGG 0.502657 2.2:-71041387 MS.gene01262:intron
GGACACACCATCTCAGACTT+AGG 0.503073 2.2:+71042498 None:intergenic
AGAGAAAGTGAGTGTTCTTG+AGG 0.506732 2.2:-71041684 MS.gene01262:CDS
CTTGAACAGCTTTAAAGACA+TGG 0.514024 2.2:+71042406 None:intergenic
TCATATTGTGTATCTGATGT+TGG 0.522543 2.2:+71042591 None:intergenic
GGTCTTTCGTTTCCGGTAGA+CGG 0.530949 2.2:+71042660 None:intergenic
TGGCAATTTGCAGATGGATG+TGG 0.531836 2.2:-71040996 MS.gene01262:intron
TGCATGGAGACTCCGTCTAC+CGG 0.552215 2.2:-71042672 MS.gene01262:CDS
TCTTGTTCCAAACCTAAAGA+AGG 0.556354 2.2:-71042271 MS.gene01262:intron
CTACATTGTTTGAAGAGTCA+AGG 0.557167 2.2:-71041497 MS.gene01262:CDS
TGTCTTTAAAGCTGTTCAAG+AGG 0.569037 2.2:-71042403 MS.gene01262:CDS
TGTGTAATTCAAATCAGCAA+AGG 0.572771 2.2:+71042542 None:intergenic
GCAAACATGTTGATCACTCA+AGG 0.574022 2.2:-71042450 MS.gene01262:CDS
AGCAATGATGGTTATATCAA+GGG 0.578216 2.2:+71041391 None:intergenic
CTTCAACAACAAAACATGCA+TGG 0.578541 2.2:-71042688 MS.gene01262:CDS
CAGACAGAGCGATGAATCGT+TGG 0.578725 2.2:+71042295 None:intergenic
TGGAGATTTCATCTATCAGA+GGG 0.587975 2.2:-71043096 MS.gene01262:CDS
GAAAGTGAGTGTTCTTGAGG+AGG 0.588414 2.2:-71041681 MS.gene01262:CDS
ATTGAAGCCTAAGTCTGAGA+TGG 0.590244 2.2:-71042505 MS.gene01262:CDS
ATGGAGATTTCATCTATCAG+AGG 0.606668 2.2:-71043097 MS.gene01262:CDS
GGTGAAATTCCCAAAAGTCA+AGG 0.621239 2.2:+71041412 None:intergenic
TATTGTGTATCTGATGTTGG+AGG 0.636554 2.2:+71042594 None:intergenic
GTCTACCGGAAACGAAAGAC+CGG 0.653717 2.2:-71042658 MS.gene01262:CDS
ATTTCTGCAGGGGATAATGG+AGG 0.671540 2.2:-71042826 MS.gene01262:intron
ATGATCACATCGTAGCGGAG+AGG 0.690331 2.2:-71042338 MS.gene01262:CDS
TGACAGTGAAGGATCTTGTG+AGG 0.718300 2.2:-71040964 MS.gene01262:CDS
AGCTCATGATCACATCGTAG+CGG 0.723921 2.2:-71042343 MS.gene01262:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAGTTAATTATCA+GGG - chr2.2:71041994-71042013 MS.gene01262:intron 10.0%
!! GAGTAATATAATTAATTACT+CGG + chr2.2:71042686-71042705 None:intergenic 15.0%
!! TCCAATATTCATATAAAATT+AGG + chr2.2:71043018-71043037 None:intergenic 15.0%
!! TTAACATTAGATATACATAT+AGG - chr2.2:71042253-71042272 MS.gene01262:intron 15.0%
!!! AAAAGTATATATTTCATCTT+AGG - chr2.2:71041833-71041852 MS.gene01262:intron 15.0%
!!! CTTTTTTAATAGTTTATCTT+AGG + chr2.2:71042119-71042138 None:intergenic 15.0%
!!! GTAATATTTCAATTTTGATT+AGG - chr2.2:71041897-71041916 MS.gene01262:intron 15.0%
!!! GTTATTTTTAGTTAATTATC+AGG - chr2.2:71041993-71042012 MS.gene01262:intron 15.0%
!!! TCCTAATTTTATATGAATAT+TGG - chr2.2:71043014-71043033 MS.gene01262:intron 15.0%
!! AAATGGTTCAAAAATAAAGT+AGG + chr2.2:71041088-71041107 None:intergenic 20.0%
!! AATATATATCTTACCATTTC+AGG + chr2.2:71040973-71040992 None:intergenic 20.0%
!! AATTGTGTATCAAGTATTAT+TGG - chr2.2:71041971-71041990 MS.gene01262:intron 20.0%
!! ATATGAATATTGGAATTGTT+TGG - chr2.2:71043024-71043043 MS.gene01262:intron 20.0%
!! ATTCATATAAAATTAGGACT+TGG + chr2.2:71043012-71043031 None:intergenic 20.0%
!! TTGATTCAAAATATGGTATT+TGG - chr2.2:71041116-71041135 MS.gene01262:intron 20.0%
!!! AGTCTAAATTGATTCAATTT+TGG + chr2.2:71041019-71041038 None:intergenic 20.0%
!!! TATATACTTTTGACAATATG+AGG + chr2.2:71041824-71041843 None:intergenic 20.0%
! CATTATTTGATACCTTCTTT+AGG + chr2.2:71041784-71041803 None:intergenic 25.0%
! TATTGGTATCAATAGAGTTT+AGG + chr2.2:71041271-71041290 None:intergenic 25.0%
! TTAGGTGATTATTAAAGTGA+TGG + chr2.2:71041253-71041272 None:intergenic 25.0%
! TTGAATCAACATGTGTTAAA+TGG + chr2.2:71041105-71041124 None:intergenic 25.0%
! TTGAATTACACAAATCAGTT+GGG - chr2.2:71041509-71041528 MS.gene01262:CDS 25.0%
! TTTGAATTACACAAATCAGT+TGG - chr2.2:71041508-71041527 MS.gene01262:CDS 25.0%
!! AATGTTTTATGATTTCTGCA+GGG - chr2.2:71041203-71041222 MS.gene01262:intron 25.0%
!! TAATGTTTTATGATTTCTGC+AGG - chr2.2:71041202-71041221 MS.gene01262:intron 25.0%
!! TATAAGGTACTTAAAGCAAA+TGG - chr2.2:71042332-71042351 MS.gene01262:CDS 25.0%
!! TTATTTCTTAACTGTTTCAC+CGG + chr2.2:71041404-71041423 None:intergenic 25.0%
!! TTTTTGTAAACACCTATTCT+TGG - chr2.2:71040992-71041011 MS.gene01262:intron 25.0%
!!! ACACATGTTGATTCAAAATA+TGG - chr2.2:71041109-71041128 MS.gene01262:intron 25.0%
!!! CTAAAGAAATTTTAACCAAG+AGG + chr2.2:71042033-71042052 None:intergenic 25.0%
!!! GATTGATTGTTATTTGTTGA+CGG + chr2.2:71042897-71042916 None:intergenic 25.0%
AACAGTTAAGAAATAACAGC+TGG - chr2.2:71041408-71041427 MS.gene01262:CDS 30.0%
AACATCGTGATATCTTTGTT+AGG - chr2.2:71041875-71041894 MS.gene01262:intron 30.0%
ACAAAAAAGGAAGAAAACTG+TGG - chr2.2:71042383-71042402 MS.gene01262:CDS 30.0%
AGCAATGATGGTTATATCAA+GGG + chr2.2:71042652-71042671 None:intergenic 30.0%
CAAAATCTTGCTTCAATTTC+AGG + chr2.2:71042501-71042520 None:intergenic 30.0%
GTTGTTGAAGTTTGGATTAT+TGG + chr2.2:71041342-71041361 None:intergenic 30.0%
TCATATTGTGTATCTGATGT+TGG + chr2.2:71041452-71041471 None:intergenic 30.0%
TGTGTAATTCAAATCAGCAA+AGG + chr2.2:71041501-71041520 None:intergenic 30.0%
! ATGTTTTATAGTTCCTCGTA+AGG + chr2.2:71041860-71041879 None:intergenic 30.0%
! ATGTTTTATGATTTCTGCAG+GGG - chr2.2:71041204-71041223 MS.gene01262:intron 30.0%
! TGAGCTTGAGAGATTTTATT+AGG + chr2.2:71041683-71041702 None:intergenic 30.0%
! TTAAAGTGATGGAGAAAGTT+AGG + chr2.2:71041242-71041261 None:intergenic 30.0%
! TTTGATACCTTCTTTAGGTT+TGG + chr2.2:71041779-71041798 None:intergenic 30.0%
! TTTTACATACTCATGATTCC+TGG + chr2.2:71041953-71041972 None:intergenic 30.0%
!! TCAAACAATGTAGTCTTATG+AGG + chr2.2:71042536-71042555 None:intergenic 30.0%
!! TTCAATTTTGGTCCAAGAAT+AGG + chr2.2:71041007-71041026 None:intergenic 30.0%
!!! ATCTAGCTTCTGAAATTTTG+TGG - chr2.2:71041157-71041176 MS.gene01262:intron 30.0%
!!! CATGTTTTGTTGTTGAAGTT+TGG + chr2.2:71041350-71041369 None:intergenic 30.0%
!!! CATGTTTTTTATGACAAACC+AGG - chr2.2:71041932-71041951 MS.gene01262:intron 30.0%
!!! GTACTAGTGTTATCGATTTT+CGG + chr2.2:71041566-71041585 None:intergenic 30.0%
AGCAGAAGCAGTTAAGTTAT+TGG + chr2.2:71041288-71041307 None:intergenic 35.0%
ATGGAGATTTCATCTATCAG+AGG - chr2.2:71040943-71040962 MS.gene01262:CDS 35.0%
ATTGTTTGGCAATTTGCAGA+TGG - chr2.2:71043038-71043057 MS.gene01262:intron 35.0%
CTACATTGTTTGAAGAGTCA+AGG - chr2.2:71042543-71042562 MS.gene01262:CDS 35.0%
CTTCAACAACAAAACATGCA+TGG - chr2.2:71041352-71041371 MS.gene01262:intron 35.0%
GAAAATTACCTGAGCAATGA+TGG + chr2.2:71042664-71042683 None:intergenic 35.0%
GAGCAATGATGGTTATATCA+AGG + chr2.2:71042653-71042672 None:intergenic 35.0%
TATTGTGTATCTGATGTTGG+AGG + chr2.2:71041449-71041468 None:intergenic 35.0%
TCTTGTTCCAAACCTAAAGA+AGG - chr2.2:71041769-71041788 MS.gene01262:intron 35.0%
TGATATAACCATCATTGCTC+AGG - chr2.2:71042653-71042672 MS.gene01262:CDS 35.0%
TGGAGATTTCATCTATCAGA+GGG - chr2.2:71040944-71040963 MS.gene01262:CDS 35.0%
TTCTTGAGGAGGAACAAAAA+AGG - chr2.2:71042370-71042389 MS.gene01262:CDS 35.0%
! AACTTAACTGCTTCTGCTTT+TGG - chr2.2:71041290-71041309 MS.gene01262:intron 35.0%
! AATAGCAGTTCCTTGACTTT+TGG - chr2.2:71042618-71042637 MS.gene01262:CDS 35.0%
! ATAGCAGTTCCTTGACTTTT+GGG - chr2.2:71042619-71042638 MS.gene01262:CDS 35.0%
! TTCAGTTTTCTGCATACAGA+TGG - chr2.2:71042284-71042303 MS.gene01262:CDS 35.0%
!! ATCACTCAAGGTAACTTGTT+TGG - chr2.2:71041602-71041621 MS.gene01262:CDS 35.0%
!! CTTGAACAGCTTTAAAGACA+TGG + chr2.2:71041637-71041656 None:intergenic 35.0%
!! TCACTCAAGGTAACTTGTTT+GGG - chr2.2:71041603-71041622 MS.gene01262:CDS 35.0%
!! TCTGAAATTTTGTGGTAGCT+AGG - chr2.2:71041165-71041184 MS.gene01262:intron 35.0%
!! TGTCTTTAAAGCTGTTCAAG+AGG - chr2.2:71041637-71041656 MS.gene01262:CDS 35.0%
AGAGAAAGTGAGTGTTCTTG+AGG - chr2.2:71042356-71042375 MS.gene01262:CDS 40.0%
ATACCAACTAACTCGGATAG+AGG - chr2.2:71042064-71042083 MS.gene01262:intron 40.0%
ATGATTTCTGCAGGGGATAA+TGG - chr2.2:71041211-71041230 MS.gene01262:intron 40.0%
ATGGACAAAGCTTCTGTTCT+TGG - chr2.2:71042303-71042322 MS.gene01262:CDS 40.0%
ATTGAAGCCTAAGTCTGAGA+TGG - chr2.2:71041535-71041554 MS.gene01262:CDS 40.0%
CAAATGTCATCTTTCCAAGC+AGG + chr2.2:71042202-71042221 None:intergenic 40.0%
CCTCGATATACCAACTAACT+CGG - chr2.2:71042057-71042076 MS.gene01262:intron 40.0%
CTGTTCTTGGAGAAGCTATA+AGG - chr2.2:71042316-71042335 MS.gene01262:CDS 40.0%
GCAAACATGTTGATCACTCA+AGG - chr2.2:71041590-71041609 MS.gene01262:CDS 40.0%
GGTGAAATTCCCAAAAGTCA+AGG + chr2.2:71042631-71042650 None:intergenic 40.0%
TATCAGAGGGTTACCTGAAA+TGG - chr2.2:71040957-71040976 MS.gene01262:CDS 40.0%
TTTCATCTTAGGTCCTTACG+AGG - chr2.2:71041844-71041863 MS.gene01262:intron 40.0%
TTTCCTCTATCCGAGTTAGT+TGG + chr2.2:71042070-71042089 None:intergenic 40.0%
! CGAGTTAGTTGGTATATCGA+GGG + chr2.2:71042059-71042078 None:intergenic 40.0%
! GGAATTTTGCATGACAGTGA+AGG - chr2.2:71043065-71043084 MS.gene01262:intron 40.0%
AGCTCATGATCACATCGTAG+CGG - chr2.2:71041697-71041716 MS.gene01262:CDS 45.0%
GAAAGTGAGTGTTCTTGAGG+AGG - chr2.2:71042359-71042378 MS.gene01262:CDS 45.0%
GGTTTGAACTCTAGACCTCT+TGG - chr2.2:71042015-71042034 MS.gene01262:intron 45.0%
TGACAGTGAAGGATCTTGTG+AGG - chr2.2:71043076-71043095 MS.gene01262:intron 45.0%
TGGCAATTTGCAGATGGATG+TGG - chr2.2:71043044-71043063 MS.gene01262:intron 45.0%
TTTCACCGGTCTTTCGTTTC+CGG + chr2.2:71041390-71041409 None:intergenic 45.0%
! ATTTCTGCAGGGGATAATGG+AGG - chr2.2:71041214-71041233 MS.gene01262:intron 45.0%
! CCGAGTTAGTTGGTATATCG+AGG + chr2.2:71042060-71042079 None:intergenic 45.0%
!! AGGTAGCTCAGTTGGTAAGT+TGG + chr2.2:71042182-71042201 None:intergenic 45.0%
!! GGTAGCTCAGTTGGTAAGTT+GGG + chr2.2:71042181-71042200 None:intergenic 45.0%
!! GTAGCTCAGTTGGTAAGTTG+GGG + chr2.2:71042180-71042199 None:intergenic 45.0%
CAGACAGAGCGATGAATCGT+TGG + chr2.2:71041748-71041767 None:intergenic 50.0%
GGACACACCATCTCAGACTT+AGG + chr2.2:71041545-71041564 None:intergenic 50.0%
GGTCTTTCGTTTCCGGTAGA+CGG + chr2.2:71041383-71041402 None:intergenic 50.0%
GTCTACCGGAAACGAAAGAC+CGG - chr2.2:71041382-71041401 MS.gene01262:intron 50.0%
TACCAACTGAGCTACCTGCT+TGG - chr2.2:71042185-71042204 MS.gene01262:intron 50.0%
TTCCAAGCAGGTAGCTCAGT+TGG + chr2.2:71042190-71042209 None:intergenic 50.0%
! ATGATCACATCGTAGCGGAG+AGG - chr2.2:71041702-71041721 MS.gene01262:CDS 50.0%
! GTAAGTTGGGGTCAGCTACA+TGG + chr2.2:71042168-71042187 None:intergenic 50.0%
TGCATGGAGACTCCGTCTAC+CGG - chr2.2:71041368-71041387 MS.gene01262:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 71040931 71043131 71040931 ID=MS.gene01262
chr2.2 mRNA 71040931 71043131 71040931 ID=MS.gene01262.t1;Parent=MS.gene01262
chr2.2 exon 71043084 71043131 71043084 ID=MS.gene01262.t1.exon1;Parent=MS.gene01262.t1
chr2.2 CDS 71043084 71043131 71043084 ID=cds.MS.gene01262.t1;Parent=MS.gene01262.t1
chr2.2 exon 71042272 71042838 71042272 ID=MS.gene01262.t1.exon2;Parent=MS.gene01262.t1
chr2.2 CDS 71042272 71042838 71042272 ID=cds.MS.gene01262.t1;Parent=MS.gene01262.t1
chr2.2 exon 71041388 71041759 71041388 ID=MS.gene01262.t1.exon3;Parent=MS.gene01262.t1
chr2.2 CDS 71041388 71041759 71041388 ID=cds.MS.gene01262.t1;Parent=MS.gene01262.t1
chr2.2 exon 71040931 71041005 71040931 ID=MS.gene01262.t1.exon4;Parent=MS.gene01262.t1
chr2.2 CDS 71040931 71041005 71040931 ID=cds.MS.gene01262.t1;Parent=MS.gene01262.t1
Gene Sequence

>MS.gene01262

ATGAGTTCTCATATGGAGATTTCATCTATCAGAGGGTTACCTGAAATGGTAAGATATATATTTTTTGTAAACACCTATTCTTGGACCAAAATTGAATCAATTTAGACTATTCAAGTTAATAGTGTGAATCATTTATTATATATGTTTACATTGACCTACTTTATTTTTGAACCATTTAACACATGTTGATTCAAAATATGGTATTTGGTTGATGAAATTTCTATGAATCTAGCTTCTGAAATTTTGTGGTAGCTAGGAGTAATTAAGAGTATAATGTTTTATGATTTCTGCAGGGGATAATGGAGGATCCTAACTTTCTCCATCACTTTAATAATCACCTAAACTCTATTGATACCAATAACTTAACTGCTTCTGCTTTTGGAGATGCTTTACAAAAACATATTTTGTCCAATAATCCAAACTTCAACAACAAAACATGCATGGAGACTCCGTCTACCGGAAACGAAAGACCGGTGAAACAGTTAAGAAATAACAGCTGGAATTACAACAATAGTCCTCCAACATCAGATACACAATATGATAATTGTTGCTCTAATAATCTTCTTTCCTTTGCTGATTTGAATTACACAAATCAGTTGGGACTATTGAAGCCTAAGTCTGAGATGGTGTGTCCGAAAATCGATAACACTAGTACTCTTGCAAACATGTTGATCACTCAAGGTAACTTGTTTGGGAATCAAAACCATGTCTTTAAAGCTGTTCAAGAGGCTAAAGATATTGAGAATCGTCCTAATAAAATCTCTCAAGCTCATGATCACATCGTAGCGGAGAGGAAGCGTCGCGAGAAGCTTAGCCAACGATTCATCGCTCTGTCTGCTCTTGTTCCAAACCTAAAGAAGGTATCAAATAATGTTGTTAATGTATTATTGCCTCATATTGTCAAAAGTATATATTTCATCTTAGGTCCTTACGAGGAACTATAAAACATCGTGATATCTTTGTTAGGTAATATTTCAATTTTGATTAGGATAAATATTAAACATGTTTTTTATGACAAACCAGGAATCATGAGTATGTAAAATTGTGTATCAAGTATTATTGGTTATTTTTAGTTAATTATCAGGGTTTGAACTCTAGACCTCTTGGTTAAAATTTCTTTAGTGTCCCTCGATATACCAACTAACTCGGATAGAGGAAAGTCAGATCACTAGAGACAGATGAAGACCTAAGATAAACTATTAAAAAAGATTTAGAGATTATAGCGTAATTTGATCCATGTAGCTGACCCCAACTTACCAACTGAGCTACCTGCTTGGAAAGATGACATTTGTGTTCAAATAATGCAGTGTCAATTTTTAAACTTAACATTAGATATACATATAGGTCTCATTTTTCAGTTTTCTGCATACAGATGGACAAAGCTTCTGTTCTTGGAGAAGCTATAAGGTACTTAAAGCAAATGGAAGAGAAAGTGAGTGTTCTTGAGGAGGAACAAAAAAGGAAGAAAACTGTGGAATCTGTTGTAATAGTGAAGAAATCTCAACTTTCTATTAATGATGCTGAAAACTCTTGTTCAGACACTGATAGTACATTTGATGAGACATTACCTGAAATTGAAGCAAGATTTTGTGAAAGAAGTGTCCTCATAAGACTACATTGTTTGAAGAGTCAAGGAGTTATTGAGAAAATTATGAGTGAAATTGAGAAACTTCATCTGAAAGTCATCAATAGCAGTTCCTTGACTTTTGGGAATTTCACCCTTGATATAACCATCATTGCTCAGGTAATTTTCCGAGTAATTAATTATATTACTCTAAGCACTATGATTAATTAGTTCACACAATATAGATATTGTTTTAAGTTACATTATTTATAAAAGTAACACAAAAATGTTGATTATACAAATTAAATTAAATACTCAGTACTCACACTCATAATATGTATTGTACATGCAACATTCTATTTTTTCAAAAAATTAATTTTAATTTTAAAAAGTTTTACACCGTCAACAAATAACAATCAATCATATATGCGAATTTAAAAGCAGTTATAATGAAAAGTAAATATTTTTAATGATCTCGCAATTATGATTGATTGATCATGTAAATAAAATTACGCCAAGTCCTAATTTTATATGAATATTGGAATTGTTTGGCAATTTGCAGATGGATGTGGAATTTTGCATGACAGTGAAGGATCTTGTGAGGAAGCTACGTTCAGCATATTCATCTTTCATGTGA
Protein sequence

>MS.gene01262.t1

MSSHMEISSIRGLPEMGIMEDPNFLHHFNNHLNSIDTNNLTASAFGDALQKHILSNNPNFNNKTCMETPSTGNERPVKQLRNNSWNYNNSPPTSDTQYDNCCSNNLLSFADLNYTNQLGLLKPKSEMVCPKIDNTSTLANMLITQGNLFGNQNHVFKAVQEAKDIENRPNKISQAHDHIVAERKRREKLSQRFIALSALVPNLKKMDKASVLGEAIRYLKQMEEKVSVLEEEQKRKKTVESVVIVKKSQLSINDAENSCSDTDSTFDETLPEIEARFCERSVLIRLHCLKSQGVIEKIMSEIEKLHLKVINSSSLTFGNFTLDITIIAQMDVEFCMTVKDLVRKLRSAYSSFM