Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38577.t1 | XP_003602850.1 | 83.2 | 327 | 47 | 2 | 1 | 325 | 1 | 321 | 4.20E-137 | 497.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38577.t1 | Q9C7T4 | 51.9 | 337 | 129 | 8 | 1 | 322 | 1 | 319 | 2.8e-57 | 223.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38577.t1 | F0V437 | 83.2 | 327 | 47 | 2 | 1 | 325 | 1 | 321 | 3.0e-137 | 497.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene38577.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene38577 | -0.820875 | 5.44E-53 | -1.69E-46 |
| MS.gene050170 | MS.gene38577 | -0.833432 | 5.35E-56 | -1.69E-46 |
| MS.gene050323 | MS.gene38577 | -0.806131 | 9.55E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene38577 | -0.800574 | 1.35E-48 | -1.69E-46 |
| MS.gene050504 | MS.gene38577 | -0.82568 | 4.11E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene38577 | -0.862309 | 5.55E-64 | -1.69E-46 |
| MS.gene050612 | MS.gene38577 | -0.815187 | 1.05E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene38577 | -0.803448 | 3.47E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene38577 | -0.812782 | 3.57E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene38577 | -0.81155 | 6.62E-51 | -1.69E-46 |
| MS.gene050904 | MS.gene38577 | -0.814321 | 1.63E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene38577 | -0.801042 | 1.09E-48 | -1.69E-46 |
| MS.gene050986 | MS.gene38577 | -0.810843 | 9.42E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene38577 | 0.813078 | 3.07E-51 | -1.69E-46 |
| MS.gene051317 | MS.gene38577 | -0.815343 | 9.70E-52 | -1.69E-46 |
| MS.gene051439 | MS.gene38577 | 0.817019 | 4.10E-52 | -1.69E-46 |
| MS.gene051517 | MS.gene38577 | 0.801855 | 7.40E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene38577 | 0.816183 | 6.31E-52 | -1.69E-46 |
| MS.gene051973 | MS.gene38577 | 0.826322 | 2.89E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene38577 | -0.81663 | 5.01E-52 | -1.69E-46 |
| MS.gene052494 | MS.gene38577 | 0.830428 | 2.95E-55 | -1.69E-46 |
| MS.gene052606 | MS.gene38577 | 0.81788 | 2.62E-52 | -1.69E-46 |
| MS.gene052705 | MS.gene38577 | 0.805335 | 1.40E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene38577 | -0.812075 | 5.09E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene38577 | -0.80568 | 1.19E-49 | -1.69E-46 |
| MS.gene053097 | MS.gene38577 | 0.839207 | 1.82E-57 | -1.69E-46 |
| MS.gene053203 | MS.gene38577 | -0.82937 | 5.35E-55 | -1.69E-46 |
| MS.gene053479 | MS.gene38577 | -0.809458 | 1.87E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene38577 | -0.878595 | 2.47E-69 | -1.69E-46 |
| MS.gene054884 | MS.gene38577 | 0.810182 | 1.31E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene38577 | -0.803438 | 3.49E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene38577 | 0.813847 | 2.08E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene38577 | -0.807786 | 4.26E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene38577 | -0.804155 | 2.47E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene38577 | -0.852466 | 4.53E-61 | -1.69E-46 |
| MS.gene055750 | MS.gene38577 | -0.817778 | 2.76E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene38577 | -0.846137 | 2.62E-59 | -1.69E-46 |
| MS.gene05589 | MS.gene38577 | -0.848543 | 5.73E-60 | -1.69E-46 |
| MS.gene056266 | MS.gene38577 | -0.825698 | 4.07E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene38577 | -0.822621 | 2.15E-53 | -1.69E-46 |
| MS.gene056678 | MS.gene38577 | 0.817742 | 2.82E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene38577 | -0.805409 | 1.35E-49 | -1.69E-46 |
| MS.gene056694 | MS.gene38577 | 0.854599 | 1.11E-61 | -1.69E-46 |
| MS.gene056697 | MS.gene38577 | 0.816778 | 4.64E-52 | -1.69E-46 |
| MS.gene056748 | MS.gene38577 | 0.807827 | 4.18E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene38577 | 0.815576 | 8.61E-52 | -1.69E-46 |
| MS.gene056988 | MS.gene38577 | 0.818091 | 2.35E-52 | -1.69E-46 |
| MS.gene057658 | MS.gene38577 | -0.818285 | 2.12E-52 | -1.69E-46 |
| MS.gene057768 | MS.gene38577 | 0.835532 | 1.59E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene38577 | -0.81772 | 2.85E-52 | -1.69E-46 |
| MS.gene058334 | MS.gene38577 | 0.828971 | 6.68E-55 | -1.69E-46 |
| MS.gene058418 | MS.gene38577 | 0.813517 | 2.46E-51 | -1.69E-46 |
| MS.gene058452 | MS.gene38577 | 0.800431 | 1.45E-48 | -1.69E-46 |
| MS.gene058544 | MS.gene38577 | -0.820506 | 6.62E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene38577 | -0.818606 | 1.80E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene38577 | -0.812358 | 4.41E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene38577 | -0.822106 | 2.83E-53 | -1.69E-46 |
| MS.gene05926 | MS.gene38577 | 0.805287 | 1.44E-49 | -1.69E-46 |
| MS.gene059667 | MS.gene38577 | -0.811178 | 7.97E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene38577 | -0.804294 | 2.31E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene38577 | -0.820789 | 5.70E-53 | -1.69E-46 |
| MS.gene06000 | MS.gene38577 | -0.846657 | 1.89E-59 | -1.69E-46 |
| MS.gene060087 | MS.gene38577 | 0.817872 | 2.63E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene38577 | -0.815908 | 7.26E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene38577 | -0.832603 | 8.60E-56 | -1.69E-46 |
| MS.gene06080 | MS.gene38577 | 0.801842 | 7.44E-49 | -1.69E-46 |
| MS.gene06084 | MS.gene38577 | 0.803248 | 3.82E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene38577 | -0.837611 | 4.69E-57 | -1.69E-46 |
| MS.gene061038 | MS.gene38577 | 0.805751 | 1.15E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene38577 | 0.843144 | 1.68E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene047441 | MS.gene38577 | PPI |
| MS.gene38577 | MS.gene02239 | PPI |
| MS.gene38577 | MS.gene028530 | PPI |
| MS.gene38577 | MS.gene40107 | PPI |
| MS.gene38577 | MS.gene002282 | PPI |
| MS.gene38577 | MS.gene01262 | PPI |
| MS.gene38577 | MS.gene90276 | PPI |
| MS.gene38577 | MS.gene053631 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38577.t1 | MTR_3g099620 | 94.495 | 327 | 10 | 2 | 1 | 325 | 1 | 321 | 0.0 | 638 |
| MS.gene38577.t1 | MTR_5g064950 | 56.098 | 328 | 106 | 10 | 1 | 321 | 1 | 297 | 3.59e-107 | 315 |
| MS.gene38577.t1 | MTR_7g113830 | 51.250 | 320 | 131 | 6 | 1 | 319 | 14 | 309 | 7.60e-97 | 289 |
| MS.gene38577.t1 | MTR_1g082480 | 50.755 | 331 | 140 | 6 | 1 | 319 | 14 | 333 | 1.77e-93 | 281 |
| MS.gene38577.t1 | MTR_5g064950 | 55.000 | 300 | 97 | 10 | 1 | 293 | 1 | 269 | 8.16e-93 | 278 |
| MS.gene38577.t1 | MTR_1g082480 | 50.820 | 305 | 127 | 6 | 1 | 293 | 14 | 307 | 1.07e-83 | 256 |
| MS.gene38577.t1 | MTR_8g066770 | 57.674 | 215 | 74 | 3 | 111 | 319 | 103 | 306 | 2.53e-80 | 247 |
| MS.gene38577.t1 | MTR_8g024210 | 52.427 | 206 | 84 | 3 | 111 | 316 | 170 | 361 | 2.87e-58 | 192 |
| MS.gene38577.t1 | MTR_0194s0050 | 47.556 | 225 | 93 | 6 | 103 | 313 | 165 | 378 | 2.38e-56 | 187 |
| MS.gene38577.t1 | MTR_2g022280 | 41.146 | 192 | 102 | 4 | 122 | 311 | 1 | 183 | 1.03e-39 | 138 |
| MS.gene38577.t1 | MTR_3g090850 | 34.661 | 251 | 122 | 6 | 102 | 311 | 77 | 326 | 1.62e-37 | 137 |
| MS.gene38577.t1 | MTR_1g028470 | 36.437 | 247 | 127 | 7 | 85 | 311 | 54 | 290 | 4.47e-36 | 132 |
| MS.gene38577.t1 | MTR_1g112680 | 26.066 | 211 | 122 | 7 | 125 | 314 | 319 | 516 | 1.97e-12 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38577.t1 | AT1G72210 | 49.560 | 341 | 131 | 9 | 1 | 322 | 1 | 319 | 3.42e-91 | 275 |
| MS.gene38577.t1 | AT1G22490 | 46.084 | 332 | 137 | 9 | 1 | 319 | 1 | 303 | 1.04e-85 | 260 |
| MS.gene38577.t1 | AT1G72210 | 48.397 | 312 | 120 | 9 | 1 | 293 | 1 | 290 | 7.96e-76 | 235 |
| MS.gene38577.t1 | AT1G22490 | 44.194 | 310 | 131 | 9 | 1 | 297 | 1 | 281 | 1.33e-70 | 223 |
| MS.gene38577.t1 | AT4G01460 | 41.784 | 213 | 118 | 1 | 110 | 322 | 103 | 309 | 3.18e-53 | 177 |
| MS.gene38577.t1 | AT4G01460 | 41.784 | 213 | 118 | 1 | 110 | 322 | 103 | 309 | 3.18e-53 | 177 |
| MS.gene38577.t1 | AT5G65320 | 47.059 | 204 | 100 | 3 | 118 | 319 | 98 | 295 | 3.39e-52 | 174 |
| MS.gene38577.t1 | AT3G24140 | 44.196 | 224 | 108 | 4 | 101 | 313 | 176 | 393 | 1.27e-51 | 176 |
| MS.gene38577.t1 | AT3G24140 | 44.196 | 224 | 108 | 4 | 101 | 313 | 176 | 393 | 1.83e-51 | 176 |
| MS.gene38577.t1 | AT2G46810 | 46.040 | 202 | 92 | 3 | 113 | 313 | 112 | 297 | 9.38e-51 | 170 |
| MS.gene38577.t1 | AT2G46810 | 46.040 | 202 | 92 | 3 | 113 | 313 | 172 | 357 | 2.63e-50 | 171 |
| MS.gene38577.t1 | AT2G46810 | 46.040 | 202 | 92 | 3 | 113 | 313 | 184 | 369 | 3.19e-50 | 171 |
| MS.gene38577.t1 | AT5G46690 | 43.802 | 242 | 87 | 7 | 111 | 319 | 77 | 302 | 7.86e-49 | 166 |
| MS.gene38577.t1 | AT5G65320 | 47.568 | 185 | 90 | 2 | 110 | 293 | 90 | 268 | 1.77e-47 | 161 |
| MS.gene38577.t1 | AT3G61950 | 45.098 | 204 | 94 | 3 | 111 | 313 | 116 | 302 | 2.37e-47 | 162 |
| MS.gene38577.t1 | AT3G61950 | 45.098 | 204 | 94 | 3 | 111 | 313 | 167 | 353 | 3.98e-47 | 162 |
| MS.gene38577.t1 | AT2G46810 | 40.870 | 230 | 91 | 4 | 113 | 313 | 112 | 325 | 3.60e-45 | 157 |
| MS.gene38577.t1 | AT3G61950 | 46.471 | 170 | 74 | 2 | 111 | 280 | 116 | 268 | 6.17e-43 | 150 |
| MS.gene38577.t1 | AT3G61950 | 46.471 | 170 | 74 | 2 | 111 | 280 | 167 | 319 | 1.12e-42 | 150 |
| MS.gene38577.t1 | AT5G46690 | 43.056 | 216 | 74 | 7 | 111 | 293 | 77 | 276 | 1.21e-38 | 139 |
| MS.gene38577.t1 | AT3G06120 | 36.683 | 199 | 108 | 5 | 122 | 311 | 1 | 190 | 5.01e-32 | 119 |
| MS.gene38577.t1 | AT5G53210 | 66.154 | 65 | 22 | 0 | 120 | 184 | 100 | 164 | 5.09e-23 | 98.2 |
| MS.gene38577.t1 | AT5G53210 | 66.154 | 65 | 22 | 0 | 120 | 184 | 100 | 164 | 5.41e-23 | 98.2 |
| MS.gene38577.t1 | AT3G61950 | 69.767 | 43 | 13 | 0 | 111 | 153 | 167 | 209 | 8.96e-14 | 69.7 |
| MS.gene38577.t1 | AT3G61950 | 69.767 | 43 | 13 | 0 | 111 | 153 | 167 | 209 | 8.96e-14 | 69.7 |
Find 68 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCCCATTGTTTGTGTTCT+TGG | 0.102767 | 3.3:+82094208 | None:intergenic |
| AGAACAACATTTACAGTCTA+TGG | 0.205160 | 3.3:-82094376 | MS.gene38577:CDS |
| TGATCAACGGTGCAGATTTC+AGG | 0.214308 | 3.3:+82095579 | None:intergenic |
| GGTGACCAAGCTTCTATTAT+TGG | 0.242168 | 3.3:-82094426 | MS.gene38577:CDS |
| TTGTTGGGTTACAAAATCTT+AGG | 0.275677 | 3.3:-82094098 | MS.gene38577:CDS |
| AGCAAAATCTCCATGCAAAC+TGG | 0.293552 | 3.3:-82095653 | MS.gene38577:CDS |
| CTGGGATTCTTGTTCTGTTA+AGG | 0.317030 | 3.3:-82095634 | MS.gene38577:CDS |
| TGTTACCACGGTCGATGATA+TGG | 0.322275 | 3.3:-82094055 | MS.gene38577:CDS |
| CTGCTGTGAATCAACTATTA+AGG | 0.338632 | 3.3:-82093832 | MS.gene38577:CDS |
| TTTAAGCCTATGTTCTCGTT+TGG | 0.389954 | 3.3:+82094330 | None:intergenic |
| TGCCTTCTTCTTATGTTCAA+AGG | 0.395209 | 3.3:-82095365 | MS.gene38577:CDS |
| GTTGATAAGCCAGTTCTTGT+TGG | 0.407779 | 3.3:+82093804 | None:intergenic |
| GAGAACATAGGCTTAAATAA+TGG | 0.421439 | 3.3:-82094324 | MS.gene38577:CDS |
| AACTTATGAAGATTGTTGTT+GGG | 0.423130 | 3.3:-82094113 | MS.gene38577:CDS |
| GCCTTCTTCTTATGTTCAAA+GGG | 0.428871 | 3.3:-82095364 | MS.gene38577:intron |
| CAACTTATGAAGATTGTTGT+TGG | 0.446451 | 3.3:-82094114 | MS.gene38577:CDS |
| TTCTGTTAAGGATCATCAAT+GGG | 0.447040 | 3.3:-82095622 | MS.gene38577:CDS |
| GTTCATTGTTGATGATTCCT+AGG | 0.461075 | 3.3:+82095676 | None:intergenic |
| TGCAGTTGCTGTTGCTTCCA+CGG | 0.462288 | 3.3:+82095527 | None:intergenic |
| GAACAACATTTACAGTCTAT+GGG | 0.463577 | 3.3:-82094375 | MS.gene38577:CDS |
| GTTCTGTTAAGGATCATCAA+TGG | 0.470917 | 3.3:-82095623 | MS.gene38577:CDS |
| TGATTGGAGCAGGTAGAGGA+GGG | 0.476683 | 3.3:-82093887 | MS.gene38577:intron |
| ACAATCTTCATAAGTTGTCC+TGG | 0.483622 | 3.3:+82094120 | None:intergenic |
| AAGGACGGTCCAACAAGAAC+TGG | 0.486926 | 3.3:-82093813 | MS.gene38577:CDS |
| TTGATTGGAGCAGGTAGAGG+AGG | 0.489389 | 3.3:-82093888 | MS.gene38577:intron |
| CTCCTCCAATAATAGAAGCT+TGG | 0.501188 | 3.3:+82094421 | None:intergenic |
| TCTTTGTAACTATATGTGAA+TGG | 0.514401 | 3.3:+82095765 | None:intergenic |
| AACAAGAATCCCAGTTTGCA+TGG | 0.514438 | 3.3:+82095643 | None:intergenic |
| AAAGTGAAGAATTCAGCAAA+AGG | 0.515194 | 3.3:+82094297 | None:intergenic |
| GGTAACATTAAGATGAAGAA+TGG | 0.520032 | 3.3:+82094071 | None:intergenic |
| GTTGCTTCCACGGTGGAAGA+AGG | 0.520106 | 3.3:+82095537 | None:intergenic |
| GACCAAGCTTCTATTATTGG+AGG | 0.520738 | 3.3:-82094423 | MS.gene38577:CDS |
| AAGAGGAGATTGAAAATCAA+AGG | 0.536216 | 3.3:-82095461 | MS.gene38577:CDS |
| GAACAAGAGAATGTTCTCCT+AGG | 0.539799 | 3.3:-82095693 | MS.gene38577:CDS |
| TTACCAAGAACACAAACAAT+GGG | 0.539923 | 3.3:-82094211 | MS.gene38577:CDS |
| ACCCTTTGAACATAAGAAGA+AGG | 0.558671 | 3.3:+82095363 | None:intergenic |
| TCTTTACTCAGTTAGTGTCA+AGG | 0.560473 | 3.3:-82094031 | MS.gene38577:intron |
| GATTGGAGCAGGTAGAGGAG+GGG | 0.565920 | 3.3:-82093886 | MS.gene38577:intron |
| ATTACCAAGAACACAAACAA+TGG | 0.566449 | 3.3:-82094212 | MS.gene38577:CDS |
| AATAATGGCTCTTGAAACTG+TGG | 0.572614 | 3.3:-82095805 | None:intergenic |
| ATGTGAATGGATCTTGTTGT+GGG | 0.578863 | 3.3:+82095778 | None:intergenic |
| GCAAAATCTCCATGCAAACT+GGG | 0.580662 | 3.3:-82095652 | MS.gene38577:CDS |
| CACCGTTGATCAAACTATCA+CGG | 0.598610 | 3.3:-82095568 | MS.gene38577:CDS |
| AGGAGCAATAAACTTTGTGA+AGG | 0.602345 | 3.3:-82094403 | MS.gene38577:CDS |
| CAAGAGTGCAAAGAACAAAG+AGG | 0.609385 | 3.3:-82095478 | MS.gene38577:CDS |
| AAACAACCAAACGAGAACAT+AGG | 0.609692 | 3.3:-82094336 | MS.gene38577:CDS |
| AACGGTGCAGATTTCAGGAG+AGG | 0.610812 | 3.3:+82095584 | None:intergenic |
| GGGGAGTCAGCTGAATAGTG+TGG | 0.613845 | 3.3:-82093867 | MS.gene38577:CDS |
| TCCACGGTGGAAGAAGGAGG+AGG | 0.617851 | 3.3:+82095543 | None:intergenic |
| TCTTCATCTTAATGTTACCA+CGG | 0.620808 | 3.3:-82094067 | MS.gene38577:CDS |
| CAACAACAACAACGTCACAA+TGG | 0.620930 | 3.3:-82094244 | MS.gene38577:CDS |
| AACAACATTTACAGTCTATG+GGG | 0.630582 | 3.3:-82094374 | MS.gene38577:CDS |
| CAACAGCAACTGCAACGTGT+AGG | 0.632313 | 3.3:-82095518 | MS.gene38577:CDS |
| GCTTCCACGGTGGAAGAAGG+AGG | 0.636333 | 3.3:+82095540 | None:intergenic |
| AGCCGTGATAGTTTGATCAA+CGG | 0.636568 | 3.3:+82095566 | None:intergenic |
| GGCAGACATTGAAGTTACAA+TGG | 0.641899 | 3.3:-82094184 | MS.gene38577:CDS |
| GTGAAGAATTCAGCAAAAGG+TGG | 0.646381 | 3.3:+82094300 | None:intergenic |
| AGAACACAAACAATGGGCTG+TGG | 0.648246 | 3.3:-82094205 | MS.gene38577:CDS |
| TATGTGAATGGATCTTGTTG+TGG | 0.651367 | 3.3:+82095777 | None:intergenic |
| ACAACATTTACAGTCTATGG+GGG | 0.663491 | 3.3:-82094373 | MS.gene38577:CDS |
| GAATAGTGTGGATGAAATTG+CGG | 0.673702 | 3.3:-82093855 | MS.gene38577:CDS |
| TGTGAATCAACTATTAAGGA+CGG | 0.674482 | 3.3:-82093828 | MS.gene38577:CDS |
| TGACTATGATCATCTTCATG+CGG | 0.681857 | 3.3:-82095718 | MS.gene38577:CDS |
| GTTGCACTTGTTGTGTACTG+AGG | 0.689048 | 3.3:+82094273 | None:intergenic |
| TCCTCCTCCTTCTTCCACCG+TGG | 0.691097 | 3.3:-82095544 | MS.gene38577:CDS |
| AAGAACCATATCATCGACCG+TGG | 0.707791 | 3.3:+82094050 | None:intergenic |
| CAACATTTACAGTCTATGGG+GGG | 0.730568 | 3.3:-82094372 | MS.gene38577:CDS |
| AGTTGCTGTTGCTTCCACGG+TGG | 0.802421 | 3.3:+82095530 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTTAAAAATTAAA+TGG | + | chr3.3:82095635-82095654 | None:intergenic | 0.0% |
| !! | AAATATAATTAGAAAGTTAA+CGG | + | chr3.3:82094443-82094462 | None:intergenic | 10.0% |
| !!! | AATTTTTATTTATTGATTGT+TGG | - | chr3.3:82094751-82094770 | MS.gene38577:intron | 10.0% |
| !!! | TAAATATGCATTTTTTATAA+TGG | + | chr3.3:82094929-82094948 | None:intergenic | 10.0% |
| !!! | TTAAAATTATTTATTTCTGT+TGG | - | chr3.3:82095606-82095625 | MS.gene38577:CDS | 10.0% |
| !!! | TTAATTAATTTATGAGTTTT+GGG | - | chr3.3:82095680-82095699 | MS.gene38577:CDS | 10.0% |
| !! | AATATAATTAGAAAGTTAAC+GGG | + | chr3.3:82094442-82094461 | None:intergenic | 15.0% |
| !! | TATTTGCATATTTATTTATG+TGG | - | chr3.3:82094726-82094745 | MS.gene38577:intron | 15.0% |
| !! | TTTAAGCTCTTAAAAAAATT+AGG | + | chr3.3:82094805-82094824 | None:intergenic | 15.0% |
| !!! | AGTAGAAATTTTAAGTAATT+TGG | - | chr3.3:82095657-82095676 | MS.gene38577:CDS | 15.0% |
| !!! | GTTAATTAATTTATGAGTTT+TGG | - | chr3.3:82095679-82095698 | MS.gene38577:CDS | 15.0% |
| !!! | TACAATTTTTTTTTTTGCAA+AGG | - | chr3.3:82094971-82094990 | MS.gene38577:intron | 15.0% |
| !! | AATTGTTATGTGAGTTAAAA+TGG | - | chr3.3:82095092-82095111 | MS.gene38577:intron | 20.0% |
| !!! | ATTTGTTTTACTTATGATAG+AGG | - | chr3.3:82094523-82094542 | MS.gene38577:intron | 20.0% |
| ! | ACACTAGTAGTACATAAAAA+GGG | - | chr3.3:82094837-82094856 | MS.gene38577:intron | 25.0% |
| ! | ACATTCAAAGTGAAAATTGA+AGG | + | chr3.3:82094472-82094491 | None:intergenic | 25.0% |
| ! | AGCTAACACTCAATAAAAAT+TGG | - | chr3.3:82094619-82094638 | MS.gene38577:intron | 25.0% |
| ! | ATACAAAATTCCATGTGTAT+TGG | - | chr3.3:82095135-82095154 | MS.gene38577:intron | 25.0% |
| ! | CATTCAAAGTGAAAATTGAA+GGG | + | chr3.3:82094471-82094490 | None:intergenic | 25.0% |
| ! | TCTTTGTAACTATATGTGAA+TGG | + | chr3.3:82093835-82093854 | None:intergenic | 25.0% |
| ! | TGTATTGGATTGAAAAAACA+GGG | - | chr3.3:82095150-82095169 | MS.gene38577:intron | 25.0% |
| !! | AACTTATGAAGATTGTTGTT+GGG | - | chr3.3:82095484-82095503 | MS.gene38577:CDS | 25.0% |
| !! | AAGTTGTCTTTAAAGAATTG+TGG | - | chr3.3:82094589-82094608 | MS.gene38577:intron | 25.0% |
| !! | AGTTGTCTTTAAAGAATTGT+GGG | - | chr3.3:82094590-82094609 | MS.gene38577:intron | 25.0% |
| !! | ATTTTCACTTTGAATGTATG+TGG | - | chr3.3:82094475-82094494 | MS.gene38577:intron | 25.0% |
| !! | CTTGCTATGATAAAAGATTA+TGG | + | chr3.3:82094899-82094918 | None:intergenic | 25.0% |
| !! | TTTTCACTTTGAATGTATGT+GGG | - | chr3.3:82094476-82094495 | MS.gene38577:intron | 25.0% |
| !!! | TTTTTTCAATCCAATACACA+TGG | + | chr3.3:82095148-82095167 | None:intergenic | 25.0% |
| AAAGTGAAGAATTCAGCAAA+AGG | + | chr3.3:82095303-82095322 | None:intergenic | 30.0% | |
| AACAACATTTACAGTCTATG+GGG | - | chr3.3:82095223-82095242 | MS.gene38577:intron | 30.0% | |
| AAGAGGAGATTGAAAATCAA+AGG | - | chr3.3:82094136-82094155 | MS.gene38577:CDS | 30.0% | |
| AGAACAACATTTACAGTCTA+TGG | - | chr3.3:82095221-82095240 | MS.gene38577:intron | 30.0% | |
| ATTACCAAGAACACAAACAA+TGG | - | chr3.3:82095385-82095404 | MS.gene38577:CDS | 30.0% | |
| ATTCTATCCAAGAAAAAACC+AGG | - | chr3.3:82095459-82095478 | MS.gene38577:CDS | 30.0% | |
| CAACTTATGAAGATTGTTGT+TGG | - | chr3.3:82095483-82095502 | MS.gene38577:CDS | 30.0% | |
| GAACAACATTTACAGTCTAT+GGG | - | chr3.3:82095222-82095241 | MS.gene38577:intron | 30.0% | |
| GACACATAAATGCAAATCTA+TGG | - | chr3.3:82094641-82094660 | MS.gene38577:intron | 30.0% | |
| GACACTAGTAGTACATAAAA+AGG | - | chr3.3:82094836-82094855 | MS.gene38577:intron | 30.0% | |
| GAGAACATAGGCTTAAATAA+TGG | - | chr3.3:82095273-82095292 | MS.gene38577:intron | 30.0% | |
| GGTAACATTAAGATGAAGAA+TGG | + | chr3.3:82095529-82095548 | None:intergenic | 30.0% | |
| GTGTATTGGATTGAAAAAAC+AGG | - | chr3.3:82095149-82095168 | MS.gene38577:intron | 30.0% | |
| TACTTATGATAGAGGAAAGA+TGG | - | chr3.3:82094531-82094550 | MS.gene38577:intron | 30.0% | |
| TCTTCATCTTAATGTTACCA+CGG | - | chr3.3:82095530-82095549 | MS.gene38577:CDS | 30.0% | |
| TGTGAATCAACTATTAAGGA+CGG | - | chr3.3:82095769-82095788 | MS.gene38577:CDS | 30.0% | |
| TTACCAAGAACACAAACAAT+GGG | - | chr3.3:82095386-82095405 | MS.gene38577:CDS | 30.0% | |
| TTCTGTTAAGGATCATCAAT+GGG | - | chr3.3:82093975-82093994 | MS.gene38577:intron | 30.0% | |
| TTGTTGGGTTACAAAATCTT+AGG | - | chr3.3:82095499-82095518 | MS.gene38577:CDS | 30.0% | |
| ! | CTTGGATAGAATTTTCATGT+TGG | + | chr3.3:82095451-82095470 | None:intergenic | 30.0% |
| !! | TTGGTTGTTTCGTTTTCTTT+TGG | + | chr3.3:82095251-82095270 | None:intergenic | 30.0% |
| !!! | AGTTTTGGGTATGAATTGAT+TGG | - | chr3.3:82095694-82095713 | MS.gene38577:CDS | 30.0% |
| AAACAACCAAACGAGAACAT+AGG | - | chr3.3:82095261-82095280 | MS.gene38577:intron | 35.0% | |
| ACAACATTTACAGTCTATGG+GGG | - | chr3.3:82095224-82095243 | MS.gene38577:intron | 35.0% | |
| ACAATCTTCATAAGTTGTCC+TGG | + | chr3.3:82095480-82095499 | None:intergenic | 35.0% | |
| ACCCTTTGAACATAAGAAGA+AGG | + | chr3.3:82094237-82094256 | None:intergenic | 35.0% | |
| AGGAGCAATAAACTTTGTGA+AGG | - | chr3.3:82095194-82095213 | MS.gene38577:intron | 35.0% | |
| CCTAAAGCTCACTCAAAAAA+CGG | + | chr3.3:82094672-82094691 | None:intergenic | 35.0% | |
| CTGCTGTGAATCAACTATTA+AGG | - | chr3.3:82095765-82095784 | MS.gene38577:CDS | 35.0% | |
| GAATAGTGTGGATGAAATTG+CGG | - | chr3.3:82095742-82095761 | MS.gene38577:CDS | 35.0% | |
| GCCTTCTTCTTATGTTCAAA+GGG | - | chr3.3:82094233-82094252 | MS.gene38577:CDS | 35.0% | |
| GTTCTGTTAAGGATCATCAA+TGG | - | chr3.3:82093974-82093993 | MS.gene38577:intron | 35.0% | |
| TATGTGAATGGATCTTGTTG+TGG | + | chr3.3:82093823-82093842 | None:intergenic | 35.0% | |
| TCTTTACTCAGTTAGTGTCA+AGG | - | chr3.3:82095566-82095585 | MS.gene38577:CDS | 35.0% | |
| TGACTATGATCATCTTCATG+CGG | - | chr3.3:82093879-82093898 | MS.gene38577:intron | 35.0% | |
| TGCCTTCTTCTTATGTTCAA+AGG | - | chr3.3:82094232-82094251 | MS.gene38577:CDS | 35.0% | |
| TTTAAGCCTATGTTCTCGTT+TGG | + | chr3.3:82095270-82095289 | None:intergenic | 35.0% | |
| ! | ATGTGAATGGATCTTGTTGT+GGG | + | chr3.3:82093822-82093841 | None:intergenic | 35.0% |
| !! | GTTCATTGTTGATGATTCCT+AGG | + | chr3.3:82093924-82093943 | None:intergenic | 35.0% |
| !!! | AAGTTGTCCTGGTTTTTTCT+TGG | + | chr3.3:82095469-82095488 | None:intergenic | 35.0% |
| AACAAGAATCCCAGTTTGCA+TGG | + | chr3.3:82093957-82093976 | None:intergenic | 40.0% | |
| AGCAAAATCTCCATGCAAAC+TGG | - | chr3.3:82093944-82093963 | MS.gene38577:intron | 40.0% | |
| AGCCGTGATAGTTTGATCAA+CGG | + | chr3.3:82094034-82094053 | None:intergenic | 40.0% | |
| CAACAACAACAACGTCACAA+TGG | - | chr3.3:82095353-82095372 | MS.gene38577:intron | 40.0% | |
| CAACATTTACAGTCTATGGG+GGG | - | chr3.3:82095225-82095244 | MS.gene38577:intron | 40.0% | |
| CACCGTTGATCAAACTATCA+CGG | - | chr3.3:82094029-82094048 | MS.gene38577:intron | 40.0% | |
| CACTTTGAATGTATGTGGGT+AGG | - | chr3.3:82094480-82094499 | MS.gene38577:intron | 40.0% | |
| CTCCTCCAATAATAGAAGCT+TGG | + | chr3.3:82095179-82095198 | None:intergenic | 40.0% | |
| CTGGGATTCTTGTTCTGTTA+AGG | - | chr3.3:82093963-82093982 | MS.gene38577:intron | 40.0% | |
| GAACAAGAGAATGTTCTCCT+AGG | - | chr3.3:82093904-82093923 | MS.gene38577:intron | 40.0% | |
| GACCAAGCTTCTATTATTGG+AGG | - | chr3.3:82095174-82095193 | MS.gene38577:intron | 40.0% | |
| GCAAAATCTCCATGCAAACT+GGG | - | chr3.3:82093945-82093964 | MS.gene38577:intron | 40.0% | |
| GGCAGACATTGAAGTTACAA+TGG | - | chr3.3:82095413-82095432 | MS.gene38577:CDS | 40.0% | |
| GGTGACCAAGCTTCTATTAT+TGG | - | chr3.3:82095171-82095190 | MS.gene38577:intron | 40.0% | |
| GTGAAGAATTCAGCAAAAGG+TGG | + | chr3.3:82095300-82095319 | None:intergenic | 40.0% | |
| ! | CAAGAGTGCAAAGAACAAAG+AGG | - | chr3.3:82094119-82094138 | MS.gene38577:CDS | 40.0% |
| ! | GTTGATAAGCCAGTTCTTGT+TGG | + | chr3.3:82095796-82095815 | None:intergenic | 40.0% |
| !! | TGAGTGAGCTTTAGGTTGTT+TGG | - | chr3.3:82094677-82094696 | MS.gene38577:intron | 40.0% |
| !!! | CCGTTTTTTGAGTGAGCTTT+AGG | - | chr3.3:82094669-82094688 | MS.gene38577:intron | 40.0% |
| AAGAACCATATCATCGACCG+TGG | + | chr3.3:82095550-82095569 | None:intergenic | 45.0% | |
| CAGCCCATTGTTTGTGTTCT+TGG | + | chr3.3:82095392-82095411 | None:intergenic | 45.0% | |
| GAATTGATTGGAGCAGGTAG+AGG | - | chr3.3:82095706-82095725 | MS.gene38577:CDS | 45.0% | |
| GGGTATGAATTGATTGGAGC+AGG | - | chr3.3:82095700-82095719 | MS.gene38577:CDS | 45.0% | |
| TGATCAACGGTGCAGATTTC+AGG | + | chr3.3:82094021-82094040 | None:intergenic | 45.0% | |
| TGTTACCACGGTCGATGATA+TGG | - | chr3.3:82095542-82095561 | MS.gene38577:CDS | 45.0% | |
| !! | AGAACACAAACAATGGGCTG+TGG | - | chr3.3:82095392-82095411 | MS.gene38577:CDS | 45.0% |
| !! | GTTGCACTTGTTGTGTACTG+AGG | + | chr3.3:82095327-82095346 | None:intergenic | 45.0% |
| !!! | ATTTTAAATTTACTTAATTA+TGG | - | chr3.3:82094416-82094435 | MS.gene38577:CDS | 5.0% |
| AACGGTGCAGATTTCAGGAG+AGG | + | chr3.3:82094016-82094035 | None:intergenic | 50.0% | |
| AAGGACGGTCCAACAAGAAC+TGG | - | chr3.3:82095784-82095803 | MS.gene38577:CDS | 50.0% | |
| CAACAGCAACTGCAACGTGT+AGG | - | chr3.3:82094079-82094098 | MS.gene38577:CDS | 50.0% | |
| TGATTGGAGCAGGTAGAGGA+GGG | - | chr3.3:82095710-82095729 | MS.gene38577:CDS | 50.0% | |
| TTGATTGGAGCAGGTAGAGG+AGG | - | chr3.3:82095709-82095728 | MS.gene38577:CDS | 50.0% | |
| ! | TGCAGTTGCTGTTGCTTCCA+CGG | + | chr3.3:82094073-82094092 | None:intergenic | 50.0% |
| GATTGGAGCAGGTAGAGGAG+GGG | - | chr3.3:82095711-82095730 | MS.gene38577:CDS | 55.0% | |
| GGGGAGTCAGCTGAATAGTG+TGG | - | chr3.3:82095730-82095749 | MS.gene38577:CDS | 55.0% | |
| GTTGCTTCCACGGTGGAAGA+AGG | + | chr3.3:82094063-82094082 | None:intergenic | 55.0% | |
| ! | AGTTGCTGTTGCTTCCACGG+TGG | + | chr3.3:82094070-82094089 | None:intergenic | 55.0% |
| GCTTCCACGGTGGAAGAAGG+AGG | + | chr3.3:82094060-82094079 | None:intergenic | 60.0% | |
| TCCACGGTGGAAGAAGGAGG+AGG | + | chr3.3:82094057-82094076 | None:intergenic | 60.0% | |
| TCCTCCTCCTTCTTCCACCG+TGG | - | chr3.3:82094053-82094072 | MS.gene38577:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 82093796 | 82095823 | 82093796 | ID=MS.gene38577 |
| chr3.3 | mRNA | 82093796 | 82095823 | 82093796 | ID=MS.gene38577.t1;Parent=MS.gene38577 |
| chr3.3 | exon | 82095365 | 82095823 | 82095365 | ID=MS.gene38577.t1.exon1;Parent=MS.gene38577.t1 |
| chr3.3 | CDS | 82095365 | 82095823 | 82095365 | ID=cds.MS.gene38577.t1;Parent=MS.gene38577.t1 |
| chr3.3 | exon | 82094032 | 82094448 | 82094032 | ID=MS.gene38577.t1.exon2;Parent=MS.gene38577.t1 |
| chr3.3 | CDS | 82094032 | 82094448 | 82094032 | ID=cds.MS.gene38577.t1;Parent=MS.gene38577.t1 |
| chr3.3 | exon | 82093796 | 82093897 | 82093796 | ID=MS.gene38577.t1.exon3;Parent=MS.gene38577.t1 |
| chr3.3 | CDS | 82093796 | 82093897 | 82093796 | ID=cds.MS.gene38577.t1;Parent=MS.gene38577.t1 |
| Gene Sequence |
| Protein sequence |
