AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012669

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012669.t1 MTR_4g132590 98.381 494 8 0 50 543 2 495 0.0 1013
MS.gene012669.t1 MTR_4g132590 89.509 591 10 3 1 543 8 594 0.0 952
MS.gene012669.t1 MTR_2g016470 58.921 482 189 5 66 541 137 615 0.0 592
MS.gene012669.t1 MTR_4g084570 38.478 460 241 11 87 541 126 548 6.27e-98 308
MS.gene012669.t1 MTR_1g031590 37.908 459 243 11 87 540 125 546 4.25e-97 305
MS.gene012669.t1 MTR_3g005340 38.496 452 237 12 98 543 131 547 1.56e-95 302
MS.gene012669.t1 MTR_3g086570 36.580 421 229 9 82 495 114 503 1.55e-91 291
MS.gene012669.t1 MTR_5g059260 36.122 526 271 12 22 540 92 559 2.29e-91 291
MS.gene012669.t1 MTR_3g100310 35.345 464 269 11 93 542 71 517 9.69e-91 288
MS.gene012669.t1 MTR_5g032100 37.029 451 259 11 98 541 70 502 1.20e-90 287
MS.gene012669.t1 MTR_4g100810 36.099 446 229 11 76 515 69 464 7.41e-88 281
MS.gene012669.t1 MTR_5g099010 38.131 396 198 7 116 511 122 470 2.52e-86 278
MS.gene012669.t1 MTR_4g082310 37.415 441 221 11 110 540 94 489 2.88e-84 271
MS.gene012669.t1 MTR_3g115220 36.867 415 212 7 101 511 95 463 1.26e-83 271
MS.gene012669.t1 MTR_5g012770 35.714 476 227 15 77 540 86 494 1.34e-83 269
MS.gene012669.t1 MTR_5g042200 37.045 440 224 10 110 540 82 477 2.50e-83 268
MS.gene012669.t1 MTR_5g064620 35.477 482 231 14 87 542 86 513 3.66e-78 255
MS.gene012669.t1 MTR_3g099480 34.927 481 230 13 87 541 115 538 4.69e-77 253
MS.gene012669.t1 MTR_2g059350 36.136 440 221 13 113 539 115 507 9.80e-75 246
MS.gene012669.t1 MTR_4g110030 35.160 438 228 10 113 539 116 508 2.36e-74 245
MS.gene012669.t1 MTR_3g086570 35.735 347 187 7 82 421 114 431 6.26e-74 241
MS.gene012669.t1 MTR_6g016015 34.389 442 226 11 113 539 115 507 6.78e-74 244
MS.gene012669.t1 MTR_3g096020 32.662 447 252 8 82 527 159 557 8.55e-65 222
MS.gene012669.t1 MTR_1g102030 32.353 476 253 14 85 542 60 484 1.17e-63 217
MS.gene012669.t1 MTR_4g125460 28.370 497 291 12 60 542 35 480 3.21e-62 212
MS.gene012669.t1 MTR_4g125460 28.370 497 291 12 60 542 174 619 5.92e-61 212
MS.gene012669.t1 MTR_4g019210 30.752 439 244 9 113 540 232 621 9.69e-59 206
MS.gene012669.t1 MTR_5g081690 28.571 469 271 13 86 541 94 511 1.12e-58 204
MS.gene012669.t1 MTR_3g026650 30.394 431 244 12 116 540 176 556 1.66e-51 185
MS.gene012669.t1 MTR_4g023030 30.983 468 263 16 83 540 199 616 6.54e-50 182
MS.gene012669.t1 MTR_3g026650 31.054 351 194 7 116 463 176 481 8.50e-43 159
MS.gene012669.t1 MTR_4g132680 45.417 240 40 10 207 441 1 154 1.23e-40 144
MS.gene012669.t1 MTR_1g101990 33.022 321 162 9 229 542 6 280 2.97e-39 145
MS.gene012669.t1 MTR_1g095680 73.684 114 12 2 251 364 16 111 7.84e-39 138
MS.gene012669.t1 MTR_4g010990 28.841 371 201 11 113 477 75 388 1.94e-37 144
MS.gene012669.t1 MTR_7g017330 28.986 345 196 8 100 432 83 390 3.80e-36 143
MS.gene012669.t1 MTR_4g010990 27.635 351 191 11 133 477 6 299 4.80e-31 124
MS.gene012669.t1 MTR_3g092590 30.330 333 187 9 109 432 86 382 4.66e-30 125
MS.gene012669.t1 MTR_3g092590 29.909 331 191 7 109 432 86 382 7.06e-30 124
MS.gene012669.t1 MTR_3g026650 30.000 270 141 7 116 382 176 400 3.27e-26 111
MS.gene012669.t1 MTR_1g007390 28.614 339 191 9 109 432 86 388 1.58e-24 108
MS.gene012669.t1 MTR_7g064210 34.518 197 95 4 232 425 24 189 2.64e-23 102
MS.gene012669.t1 MTR_1g101985 42.991 107 57 3 85 189 60 164 2.89e-21 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012669.t1 AT1G62330 68.927 531 156 3 13 540 125 649 0.0 746
MS.gene012669.t1 AT1G11990 68.859 517 144 4 41 540 94 610 0.0 720
MS.gene012669.t1 AT1G11990 66.031 524 125 4 41 540 94 588 0.0 683
MS.gene012669.t1 AT1G29200 56.315 483 200 4 66 541 132 610 0.0 543
MS.gene012669.t1 AT1G29200 56.315 483 200 4 66 541 16 494 0.0 541
MS.gene012669.t1 AT1G29200 56.315 483 200 4 66 541 16 494 0.0 541
MS.gene012669.t1 AT1G29200 59.198 424 169 2 118 541 1 420 2.17e-178 509
MS.gene012669.t1 AT5G65470 36.590 481 282 13 68 542 41 504 2.86e-96 302
MS.gene012669.t1 AT2G01480 38.307 449 236 10 98 541 140 552 6.81e-95 300
MS.gene012669.t1 AT1G14970 37.360 447 243 10 98 541 21 433 4.49e-93 291
MS.gene012669.t1 AT1G14970 37.963 432 232 9 113 541 9 407 6.24e-93 290
MS.gene012669.t1 AT3G26370 36.854 426 230 9 88 506 128 521 7.01e-93 295
MS.gene012669.t1 AT4G38390 37.100 469 236 11 82 542 90 507 1.26e-92 294
MS.gene012669.t1 AT1G14970 37.360 447 243 10 98 541 141 553 1.77e-91 291
MS.gene012669.t1 AT1G35510 37.054 448 248 8 96 541 142 557 3.87e-91 290
MS.gene012669.t1 AT4G24530 36.344 465 253 13 98 542 77 518 6.16e-91 288
MS.gene012669.t1 AT2G01480 39.401 401 206 7 98 495 140 506 5.68e-88 281
MS.gene012669.t1 AT4G16650 35.941 473 243 13 77 541 84 504 1.55e-84 273
MS.gene012669.t1 AT4G16650 35.941 473 243 13 77 541 84 504 1.55e-84 273
MS.gene012669.t1 AT1G14020 37.500 440 222 10 110 540 88 483 4.10e-84 270
MS.gene012669.t1 AT2G03280 36.818 440 224 9 110 539 85 480 5.48e-84 269
MS.gene012669.t1 AT1G38065 35.550 436 240 10 113 540 26 428 3.40e-83 266
MS.gene012669.t1 AT1G38065 35.550 436 240 10 113 540 36 438 5.49e-83 266
MS.gene012669.t1 AT1G38131 35.550 436 240 10 113 540 155 557 3.91e-82 268
MS.gene012669.t1 AT1G76270 33.408 449 246 8 71 515 62 461 4.73e-81 264
MS.gene012669.t1 AT1G20550 34.404 436 229 9 82 511 76 460 2.06e-80 262
MS.gene012669.t1 AT5G15740 36.073 438 224 12 113 539 106 498 1.40e-79 258
MS.gene012669.t1 AT2G03280 34.690 467 224 10 110 539 85 507 5.93e-78 254
MS.gene012669.t1 AT3G02250 35.682 440 223 12 113 539 106 498 3.14e-77 253
MS.gene012669.t1 AT5G64600 32.861 423 229 7 84 497 79 455 1.40e-68 230
MS.gene012669.t1 AT1G22460 34.383 413 214 10 87 494 135 495 6.08e-68 229
MS.gene012669.t1 AT1G04910 32.104 461 250 10 88 541 71 475 8.40e-67 225
MS.gene012669.t1 AT5G01100 30.917 469 264 11 86 542 190 610 9.11e-62 214
MS.gene012669.t1 AT2G37980 29.039 489 275 13 71 540 180 615 5.12e-60 210
MS.gene012669.t1 AT3G54100 29.425 435 255 10 86 516 195 581 1.05e-58 206
MS.gene012669.t1 AT3G26370 36.296 270 139 4 88 351 128 370 2.08e-58 199
MS.gene012669.t1 AT5G35570 30.407 467 261 14 87 540 225 640 2.39e-58 206
MS.gene012669.t1 AT2G44500 31.250 464 262 12 86 540 146 561 1.15e-57 202
MS.gene012669.t1 AT3G07900 29.044 544 320 16 6 540 86 572 4.62e-57 201
MS.gene012669.t1 AT5G63390 29.701 468 272 14 83 541 130 549 6.17e-50 181
MS.gene012669.t1 AT1G52630 28.788 396 218 12 86 475 46 383 6.38e-39 148
MS.gene012669.t1 AT3G30300 30.448 335 186 9 108 432 95 392 1.20e-36 145
MS.gene012669.t1 AT3G30300 30.448 335 186 9 108 432 95 392 1.20e-36 145
MS.gene012669.t1 AT3G03810 30.874 366 191 10 85 432 60 381 1.99e-34 138
MS.gene012669.t1 AT3G03810 30.874 366 191 10 85 432 60 381 1.99e-34 138
MS.gene012669.t1 AT1G52630 28.000 350 193 9 129 475 2 295 4.05e-32 127
MS.gene012669.t1 AT2G44500 30.897 301 159 7 86 380 146 403 1.59e-31 127

Find 140 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAAGTTGTTCACTTTAT+TGG 0.051460 8.1:-1565155 MS.gene012669:CDS
ACACTACTGTACATAAATTT+TGG 0.080188 8.1:+1566208 None:intergenic
TTCTCTTTAGTGACTAATTT+AGG 0.115144 8.1:+1564290 None:intergenic
GCTCATTCCTTATGTGAATT+TGG 0.129244 8.1:-1564722 MS.gene012669:CDS
CCTTGGTCCCTGCTTCTTCT+TGG 0.143948 8.1:-1566648 MS.gene012669:CDS
ACCAGCAGTTTCTTGTATTC+TGG 0.171103 8.1:+1564907 None:intergenic
GTTGTTCACTTTATTGGATT+TGG 0.172106 8.1:-1565149 MS.gene012669:CDS
CTAGCAAGCCTGCGCTTATT+TGG 0.201176 8.1:+1563869 None:intergenic
CTCATTCCTTATGTGAATTT+GGG 0.207573 8.1:-1564721 MS.gene012669:CDS
CATGTTCTGATCAGCAAAAT+TGG 0.221288 8.1:-1566621 MS.gene012669:CDS
TCCATTTGGAGAATTAAATT+TGG 0.226569 8.1:-1564819 MS.gene012669:CDS
AGGAAGTAATGGATACATTT+TGG 0.228640 8.1:-1566446 MS.gene012669:CDS
CGCGGGCATGCAGGTCTTAT+TGG 0.239648 8.1:-1564866 MS.gene012669:CDS
CGGCATCTTGTTGGTCCATT+TGG 0.257656 8.1:-1564833 MS.gene012669:CDS
AGGAAGTCAGCTTTCATCTT+TGG 0.272921 8.1:-1563942 MS.gene012669:CDS
CTTCCTTCTCCTTCTCGTTA+TGG 0.276961 8.1:-1567683 MS.gene012669:CDS
TCTCTTTAGTGACTAATTTA+GGG 0.277243 8.1:+1564291 None:intergenic
TGGTTCGGAGTACGTGAATT+TGG 0.279306 8.1:+1567756 None:intergenic
ATCCTCATGCTTGCTGCTCT+TGG 0.290890 8.1:-1564398 MS.gene012669:CDS
GCATTTGCCATGACTGATTC+AGG 0.299740 8.1:-1563962 MS.gene012669:CDS
TCAATGCCAGTGCAGGAAAA+TGG 0.307253 8.1:+1564654 None:intergenic
TTGTTCACTTTATTGGATTT+GGG 0.312165 8.1:-1565148 MS.gene012669:CDS
GGTTCCTGCCACAAATCTTT+TGG 0.321484 8.1:+1566670 None:intergenic
CCAGCAGTTTCTTGTATTCT+GGG 0.323818 8.1:+1564908 None:intergenic
CATGAAAGCTTCAAAATACT+TGG 0.326457 8.1:-1564777 MS.gene012669:CDS
TCTGGTTATCGAATATATTA+TGG 0.330083 8.1:-1563917 MS.gene012669:CDS
TCCCTCACTATCCGAATATC+AGG 0.331934 8.1:+1565389 None:intergenic
CGCTTATTTGGACGTATTGT+TGG 0.334892 8.1:+1563881 None:intergenic
CAGCTTTCATCTTTGGTGTC+TGG 0.339290 8.1:-1563935 MS.gene012669:CDS
ACCTGCAGCTCAAATGGTAT+TGG 0.362625 8.1:+1565107 None:intergenic
ACAACTTGACTCCTGATATT+CGG 0.369431 8.1:-1565400 MS.gene012669:CDS
TCCAAATTTAATTCTCCAAA+TGG 0.380795 8.1:+1564818 None:intergenic
CGAAGAAGAGAGGAAAGAAT+TGG 0.381135 8.1:-1564693 MS.gene012669:CDS
GTATTCTGGGAACAAATTGC+AGG 0.383617 8.1:+1564921 None:intergenic
GTAGCTGGTTCTAATTTGTA+TGG 0.388082 8.1:-1564350 MS.gene012669:CDS
CCAAAGTTGAATTGAGTAAC+CGG 0.388733 8.1:+1566236 None:intergenic
CTGCTGGTTTGCTTCTTAAG+AGG 0.406111 8.1:-1564892 MS.gene012669:CDS
AGAAGGAAGATGATCTGATT+CGG 0.406728 8.1:+1567697 None:intergenic
GGCATTGGCTGCCCATGCAT+TGG 0.418411 8.1:-1566829 MS.gene012669:CDS
ATTTGCTAATTATTCATCTC+AGG 0.422687 8.1:-1564240 MS.gene012669:intron
CACATACATTCTTTACACCT+TGG 0.424505 8.1:+1563728 None:intergenic
GAAGGAAGATGATCTGATTC+GGG 0.432766 8.1:+1567698 None:intergenic
TCCAATACCATTTGAGCTGC+AGG 0.433289 8.1:-1565108 MS.gene012669:intron
CAGCAAAATTGGGAACCTAA+TGG 0.433939 8.1:-1566610 MS.gene012669:intron
AGATGATCTGATTCGGGAGC+TGG 0.434581 8.1:+1567704 None:intergenic
CCATTGGATCGGCATCTTGT+TGG 0.435710 8.1:-1564842 MS.gene012669:CDS
TTGATTGATCCCACCATTTG+CGG 0.436523 8.1:+1566420 None:intergenic
ATATTTACCTGCAGCTCAAA+TGG 0.439821 8.1:+1565101 None:intergenic
TACCTTCTCCTTCAGAGCTC+AGG 0.441729 8.1:-1564457 MS.gene012669:CDS
CCGGTTACTCAATTCAACTT+TGG 0.444063 8.1:-1566236 MS.gene012669:CDS
CCTTCAGAGCTCAGGTCTGA+AGG 0.450269 8.1:-1564449 MS.gene012669:CDS
GTATAATTCCATAGTCAATT+CGG 0.459391 8.1:-1565452 MS.gene012669:intron
TGCAGGTCTTATTGGACCAT+TGG 0.462153 8.1:-1564858 MS.gene012669:CDS
TCAATTCGGAGACATCTATC+AGG 0.463228 8.1:-1565438 MS.gene012669:CDS
CTTCATAGGCTGTGCTGCCT+CGG 0.466333 8.1:-1563987 MS.gene012669:CDS
TCTATTGTAGTTAGCAGCAC+TGG 0.472992 8.1:-1564011 MS.gene012669:intron
AGATTTGTGGCAGGAACCCT+TGG 0.473935 8.1:-1566665 MS.gene012669:CDS
AGCATGAGGATGGATTCTTC+AGG 0.476449 8.1:+1564410 None:intergenic
GTGAATTTGGTGAGCTGGAA+CGG 0.477372 8.1:+1567769 None:intergenic
AATTTATGTACAGTAGTGTA+TGG 0.478683 8.1:-1566204 MS.gene012669:CDS
ATGGATTCTTCAGGTGTCAA+TGG 0.485741 8.1:+1564419 None:intergenic
AAGAGGTTGCGCGGGCATGC+AGG 0.489665 8.1:-1564875 MS.gene012669:CDS
ACTACAGTCTTTGGACTTGG+AGG 0.490902 8.1:-1565354 MS.gene012669:CDS
TGAGATGAATAATTAGCAAA+TGG 0.493642 8.1:+1564242 None:intergenic
ATGTTCTGATCAGCAAAATT+GGG 0.495806 8.1:-1566620 MS.gene012669:CDS
TGGTGGGATCAATCAACAAC+GGG 0.501801 8.1:-1566413 MS.gene012669:CDS
GTAATGCTGTTGTCGTGGCC+CGG 0.505025 8.1:-1566255 MS.gene012669:CDS
CGGGAGCTGGAGAGCCGGCT+CGG 0.505261 8.1:+1567717 None:intergenic
AAAACTACAGTCTTTGGACT+TGG 0.507383 8.1:-1565357 MS.gene012669:CDS
AGCCGGCTCGGACCGAGTCT+CGG 0.508114 8.1:+1567729 None:intergenic
TCTTTGGACTTGGAGGCAAT+CGG 0.510862 8.1:-1565347 MS.gene012669:CDS
AAGACAAACATATTTGTAGC+TGG 0.511487 8.1:-1564365 MS.gene012669:CDS
TTAGCAGCACTGGACTTCAT+AGG 0.513025 8.1:-1564001 MS.gene012669:CDS
CTATAAACACTTCTTGCCTT+AGG 0.515636 8.1:+1563755 None:intergenic
CCGAGTCTCGGAGAATGGTT+CGG 0.516767 8.1:+1567741 None:intergenic
CCTTCAGACCTGAGCTCTGA+AGG 0.519070 8.1:+1564449 None:intergenic
TCGGACCGAGTCTCGGAGAA+TGG 0.524446 8.1:+1567736 None:intergenic
GTATGGAGGTCGCTCACGGT+TGG 0.525649 8.1:-1564333 MS.gene012669:CDS
AAGAGCAGCAAGCATGAGGA+TGG 0.526483 8.1:+1564400 None:intergenic
TATATACCTCTGCCAATGCA+TGG 0.527491 8.1:+1566817 None:intergenic
ACTACAATAGAAGGAAGTAA+TGG 0.530681 8.1:-1566457 MS.gene012669:intron
CATGGTGTCCAAGAAGAAGC+AGG 0.531478 8.1:+1566640 None:intergenic
CTACAAATATGTTTGTCTTG+CGG 0.533769 8.1:+1564369 None:intergenic
AGTACGTGAATTTGGTGAGC+TGG 0.540415 8.1:+1567764 None:intergenic
CTCCGAGACTCGGTCCGAGC+CGG 0.542444 8.1:-1567731 MS.gene012669:CDS
GTCTTATTGGACCATTGGAT+CGG 0.546272 8.1:-1564853 MS.gene012669:CDS
AACGTGAACCAAAAGATTTG+TGG 0.547039 8.1:-1566678 MS.gene012669:CDS
CCTTCTCGTTATGGTATCGA+AGG 0.548368 8.1:-1567674 MS.gene012669:CDS
CCAGGTAACAGATGTTGACA+TGG 0.548508 8.1:-1565234 MS.gene012669:intron
TATTGGATTTGGGAATCGCT+TGG 0.548541 8.1:-1565138 MS.gene012669:CDS
TGCTTCTTAAGAGGTTGCGC+GGG 0.551538 8.1:-1564883 MS.gene012669:CDS
GGGAAATTATGCAAGGATGT+TGG 0.555862 8.1:-1566850 MS.gene012669:CDS
TATCGAATATATTATGGTGG+AGG 0.560012 8.1:-1563911 MS.gene012669:CDS
CCCAGAATACAAGAAACTGC+TGG 0.564118 8.1:-1564908 MS.gene012669:CDS
GGCTGCCCATGCATTGGCAG+AGG 0.565876 8.1:-1566823 MS.gene012669:intron
CTTCAGAGCTCAGGTCTGAA+GGG 0.566401 8.1:-1564448 MS.gene012669:CDS
ATGTGTTTCAAGTAAAAGCT+CGG 0.568622 8.1:+1565197 None:intergenic
TCATTCCTTATGTGAATTTG+GGG 0.569061 8.1:-1564720 MS.gene012669:CDS
GGTGTAAAGAATGTATGTGC+AGG 0.571881 8.1:-1563724 MS.gene012669:CDS
CTCCTGATATTCGGATAGTG+AGG 0.572428 8.1:-1565391 MS.gene012669:CDS
ATTTGTATGGAGGTCGCTCA+CGG 0.580220 8.1:-1564337 MS.gene012669:CDS
TTATGCAAGGATGTTGGCAT+TGG 0.580228 8.1:-1566844 MS.gene012669:CDS
TTGCTTCTTAAGAGGTTGCG+CGG 0.584549 8.1:-1564884 MS.gene012669:CDS
AGAGAACTTACTTTCTTCAG+CGG 0.584628 8.1:-1564273 MS.gene012669:CDS
ATATACCTCTGCCAATGCAT+GGG 0.584882 8.1:+1566818 None:intergenic
GACTAATTTAGGGTACAAGC+TGG 0.588710 8.1:+1564301 None:intergenic
CCGAACCATTCTCCGAGACT+CGG 0.588983 8.1:-1567741 MS.gene012669:CDS
GCTGGTTCTAATTTGTATGG+AGG 0.591032 8.1:-1564347 MS.gene012669:CDS
TGATTCGGGAGCTGGAGAGC+CGG 0.592818 8.1:+1567712 None:intergenic
TATTCTGGGAACAAATTGCA+GGG 0.594650 8.1:+1564922 None:intergenic
CCAACAAGATGCCGATCCAA+TGG 0.595281 8.1:+1564842 None:intergenic
TTTCAAGTAAAAGCTCGGCT+TGG 0.597881 8.1:+1565202 None:intergenic
CGCAGAAGGGAAATTATGCA+AGG 0.598798 8.1:-1566857 MS.gene012669:CDS
ATACCATAACGAGAAGGAGA+AGG 0.599116 8.1:+1567680 None:intergenic
AGCCAAGAGCAGCAAGCATG+AGG 0.599771 8.1:+1564396 None:intergenic
CAAGAAGAAGCAGGGACCAA+GGG 0.600590 8.1:+1566649 None:intergenic
AGATGTTGACATGGGAAAGG+AGG 0.602228 8.1:-1565225 MS.gene012669:CDS
CTGACTTCCTGAATCAGTCA+TGG 0.602436 8.1:+1563955 None:intergenic
ACATGTACAGACAAGGCCTA+AGG 0.603476 8.1:-1563771 MS.gene012669:CDS
CCAAGAAGAAGCAGGGACCA+AGG 0.605073 8.1:+1566648 None:intergenic
CAATACGTCCAAATAAGCGC+AGG 0.605551 8.1:-1563877 MS.gene012669:CDS
GTTCAGGTATGGACGTAGAT+CGG 0.606473 8.1:-1567794 None:intergenic
AACAGATGTTGACATGGGAA+AGG 0.607086 8.1:-1565228 MS.gene012669:CDS
TGAACCAAAAGATTTGTGGC+AGG 0.613832 8.1:-1566674 MS.gene012669:CDS
CAAAGTTGAATTGAGTAACC+GGG 0.614622 8.1:+1566237 None:intergenic
CCTTCGATACCATAACGAGA+AGG 0.620271 8.1:+1567674 None:intergenic
TCCTGATATTCGGATAGTGA+GGG 0.625925 8.1:-1565390 MS.gene012669:CDS
GGTTATCGAATATATTATGG+TGG 0.638956 8.1:-1563914 MS.gene012669:CDS
CATTCCTTATGTGAATTTGG+GGG 0.642456 8.1:-1564719 MS.gene012669:CDS
AGTCATGGCAAATGCATCCG+AGG 0.654270 8.1:+1563970 None:intergenic
ATGGTGTCCAAGAAGAAGCA+GGG 0.657952 8.1:+1566641 None:intergenic
GGTTTGTAATGCTGTTGTCG+TGG 0.662245 8.1:-1566260 MS.gene012669:CDS
ATGGTGGGATCAATCAACAA+CGG 0.673611 8.1:-1566414 MS.gene012669:CDS
TCTCAGATTTGAAATTGACA+TGG 0.677789 8.1:-1564747 MS.gene012669:CDS
CAGGTAACAGATGTTGACAT+GGG 0.679242 8.1:-1565233 MS.gene012669:CDS
ATTCGGAGACATCTATCAGG+AGG 0.682186 8.1:-1565435 MS.gene012669:CDS
GACCTGAGCTCTGAAGGAGA+AGG 0.682474 8.1:+1564455 None:intergenic
GAAGTGTTTATAGATATCCA+AGG 0.690681 8.1:-1563745 MS.gene012669:CDS
AAACTAAACATGTACAGACA+AGG 0.694433 8.1:-1563778 MS.gene012669:CDS
GTAGTGTATGGAGAGATACA+AGG 0.697044 8.1:-1566192 MS.gene012669:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATAAGTTAAATTGA+CGG + chr8.1:1566022-1566041 None:intergenic 10.0%
!!! ATTTTGAAACAAATTTTTTT+TGG + chr8.1:1565767-1565786 None:intergenic 10.0%
!! AAATTATCCTTATTAACTAT+TGG + chr8.1:1565895-1565914 None:intergenic 15.0%
!! AATAATTTCTATAGATAGAT+AGG + chr8.1:1564054-1564073 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG - chr8.1:1565894-1565913 MS.gene012669:intron 15.0%
!! TCTAACAATTAAATACAAAT+AGG + chr8.1:1566239-1566258 None:intergenic 15.0%
!!! ACTTGATTTTTAAGTATAAT+AGG + chr8.1:1566334-1566353 None:intergenic 15.0%
!!! ATAAGATTTATCAACAATTT+TGG - chr8.1:1565545-1565564 MS.gene012669:intron 15.0%
!!! TAAGATTTATCAACAATTTT+GGG - chr8.1:1565546-1565565 MS.gene012669:intron 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG + chr8.1:1565871-1565890 None:intergenic 15.0%
!!! TTCTGATATTTATTTTTTCA+GGG - chr8.1:1564589-1564608 MS.gene012669:intron 15.0%
!!! TTTCTGATATTTATTTTTTC+AGG - chr8.1:1564588-1564607 MS.gene012669:intron 15.0%
!! AAACCTAATATTTAGTTACT+GGG - chr8.1:1565415-1565434 MS.gene012669:CDS 20.0%
!! AAACTAACCAATAGTTAATA+AGG - chr8.1:1565885-1565904 MS.gene012669:intron 20.0%
!! AAATTTAATTCAACCAATTC+TGG + chr8.1:1565506-1565525 None:intergenic 20.0%
!! AATGATTAAGAGAAAGATAA+TGG + chr8.1:1565928-1565947 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr8.1:1565808-1565827 None:intergenic 20.0%
!! TTAAATGCTTATAGTGTAAT+CGG + chr8.1:1564510-1564529 None:intergenic 20.0%
!!! AAGATTTATCAACAATTTTG+GGG - chr8.1:1565547-1565566 MS.gene012669:intron 20.0%
!!! CATCAAATAAAAAGTACATA+GGG + chr8.1:1565395-1565414 None:intergenic 20.0%
!!! CTACTTTGATTTAAAGTAAT+TGG - chr8.1:1567005-1567024 MS.gene012669:intron 20.0%
!!! GTTTAATAGTTTAACTTGTT+TGG - chr8.1:1564176-1564195 MS.gene012669:intron 20.0%
!!! TAAGCTTTATCTTAAATGAT+TGG - chr8.1:1565188-1565207 MS.gene012669:CDS 20.0%
!!! TATAAGCTATTTCTATAACA+AGG + chr8.1:1564467-1564486 None:intergenic 20.0%
!!! TCATCAAATAAAAAGTACAT+AGG + chr8.1:1565396-1565415 None:intergenic 20.0%
! AACTTATTCGTGTATTTGTT+GGG - chr8.1:1565622-1565641 MS.gene012669:intron 25.0%
! AATTTATGTACAGTAGTGTA+TGG - chr8.1:1565297-1565316 MS.gene012669:intron 25.0%
! AGTTTAATATAGTTCAAGCA+AGG + chr8.1:1564345-1564364 None:intergenic 25.0%
! ATGTCGTAATAAACAATCTA+AGG + chr8.1:1564082-1564101 None:intergenic 25.0%
! GAAAATTGAAAACTTTGCTA+TGG + chr8.1:1564763-1564782 None:intergenic 25.0%
! GAAACCTAATATTTAGTTAC+TGG - chr8.1:1565414-1565433 MS.gene012669:CDS 25.0%
! GTATAATTCCATAGTCAATT+CGG - chr8.1:1566049-1566068 MS.gene012669:intron 25.0%
! TAACTTATTCGTGTATTTGT+TGG - chr8.1:1565621-1565640 MS.gene012669:intron 25.0%
! TAGCTGTAAGTATTCTTTAT+CGG - chr8.1:1565111-1565130 MS.gene012669:CDS 25.0%
! TCCAAATTTAATTCTCCAAA+TGG + chr8.1:1566686-1566705 None:intergenic 25.0%
! TCCATTTGGAGAATTAAATT+TGG - chr8.1:1566682-1566701 MS.gene012669:CDS 25.0%
! TCTCTTTAGTGACTAATTTA+GGG + chr8.1:1567213-1567232 None:intergenic 25.0%
! TCTGGTTATCGAATATATTA+TGG - chr8.1:1567584-1567603 MS.gene012669:intron 25.0%
! TGAAAAACACTACAATAGAA+TGG - chr8.1:1567663-1567682 MS.gene012669:CDS 25.0%
! TGACAGTAAGTAACAATTTA+CGG + chr8.1:1564232-1564251 None:intergenic 25.0%
! TGAGATGAATAATTAGCAAA+TGG + chr8.1:1567262-1567281 None:intergenic 25.0%
! TTCTCTTTAGTGACTAATTT+AGG + chr8.1:1567214-1567233 None:intergenic 25.0%
! TTGTTCACTTTATTGGATTT+GGG - chr8.1:1566353-1566372 MS.gene012669:intron 25.0%
!! AATCAAGTTGTTCACTTTAT+TGG - chr8.1:1566346-1566365 MS.gene012669:intron 25.0%
!! ACACTACTGTACATAAATTT+TGG + chr8.1:1565296-1565315 None:intergenic 25.0%
!! AGATTTATCAACAATTTTGG+GGG - chr8.1:1565548-1565567 MS.gene012669:intron 25.0%
!! AGTTTAATTAGCTTACTACA+AGG + chr8.1:1566203-1566222 None:intergenic 25.0%
!! ATTTGCTAATTATTCATCTC+AGG - chr8.1:1567261-1567280 MS.gene012669:intron 25.0%
!! CAATTAGGAAAAGCATAATA+GGG + chr8.1:1566517-1566536 None:intergenic 25.0%
!! CACTACTGTACATAAATTTT+GGG + chr8.1:1565295-1565314 None:intergenic 25.0%
!! GTTTAATTAGCTTACTACAA+GGG + chr8.1:1566202-1566221 None:intergenic 25.0%
!! TATTATGCTTTTCCTAATTG+AGG - chr8.1:1566517-1566536 MS.gene012669:intron 25.0%
!! TCAATTAGGAAAAGCATAAT+AGG + chr8.1:1566518-1566537 None:intergenic 25.0%
!!! GATTTTTAAGTATAATAGGC+AGG + chr8.1:1566330-1566349 None:intergenic 25.0%
AAACAGGATTTACTGTAACA+AGG + chr8.1:1567348-1567367 None:intergenic 30.0%
AAACTAAACATGTACAGACA+AGG - chr8.1:1567723-1567742 MS.gene012669:CDS 30.0%
AAGACAAACATATTTGTAGC+TGG - chr8.1:1567136-1567155 MS.gene012669:intron 30.0%
AATTGTTAGAAGACTTTACC+AGG - chr8.1:1566249-1566268 MS.gene012669:CDS 30.0%
ACAACACTTCTGAATCATTT+GGG - chr8.1:1565134-1565153 MS.gene012669:CDS 30.0%
ACTAAGAAAAAAACGCAGAA+GGG - chr8.1:1564631-1564650 MS.gene012669:intron 30.0%
ACTACAATAGAAGGAAGTAA+TGG - chr8.1:1565044-1565063 MS.gene012669:intron 30.0%
ACTATATCAAGCAAGTACAT+AGG - chr8.1:1564383-1564402 MS.gene012669:CDS 30.0%
ATATGAGAATTGAAACCTGA+AGG + chr8.1:1563996-1564015 None:intergenic 30.0%
ATGTTCTGATCAGCAAAATT+GGG - chr8.1:1564881-1564900 MS.gene012669:CDS 30.0%
CATGAAAGCTTCAAAATACT+TGG - chr8.1:1566724-1566743 MS.gene012669:intron 30.0%
CTACAAATATGTTTGTCTTG+CGG + chr8.1:1567135-1567154 None:intergenic 30.0%
CTCATTCCTTATGTGAATTT+GGG - chr8.1:1566780-1566799 MS.gene012669:intron 30.0%
CTTACTGTCATAAGTTCTTA+TGG - chr8.1:1564242-1564261 MS.gene012669:CDS 30.0%
GAAATTTGATCAACCAGAAT+TGG - chr8.1:1565490-1565509 MS.gene012669:intron 30.0%
GGCTTATATAACTACAAACT+AGG + chr8.1:1564726-1564745 None:intergenic 30.0%
GGTTATCGAATATATTATGG+TGG - chr8.1:1567587-1567606 MS.gene012669:intron 30.0%
GTTCAAAAGGAAACAGATAT+AGG + chr8.1:1564556-1564575 None:intergenic 30.0%
GTTGTTCACTTTATTGGATT+TGG - chr8.1:1566352-1566371 MS.gene012669:intron 30.0%
TAAAACAAGAATGCTAGAAG+TGG + chr8.1:1565603-1565622 None:intergenic 30.0%
TAATGACAATGAGAGAGAAA+TGG + chr8.1:1565346-1565365 None:intergenic 30.0%
TACTAAGAAAAAAACGCAGA+AGG - chr8.1:1564630-1564649 MS.gene012669:intron 30.0%
TATCGAATATATTATGGTGG+AGG - chr8.1:1567590-1567609 MS.gene012669:intron 30.0%
TATGAGAATTGAAACCTGAA+GGG + chr8.1:1563995-1564014 None:intergenic 30.0%
TCAAATAAGTCAATCCAAAC+AGG - chr8.1:1564277-1564296 MS.gene012669:CDS 30.0%
TCACCCAGTAACTAAATATT+AGG + chr8.1:1565421-1565440 None:intergenic 30.0%
TCATTCCTTATGTGAATTTG+GGG - chr8.1:1566781-1566800 MS.gene012669:intron 30.0%
TCCATAGCTGCAAAAAAAAA+AGG - chr8.1:1564969-1564988 MS.gene012669:intron 30.0%
TCCTAAAAAACTACAGTCTT+TGG - chr8.1:1566138-1566157 MS.gene012669:intron 30.0%
TTAAATGATTGGCGTCATTT+GGG - chr8.1:1565199-1565218 MS.gene012669:CDS 30.0%
TTCTACTCTACTACAATAGA+AGG - chr8.1:1565035-1565054 MS.gene012669:intron 30.0%
TTGTGATGTCTAATTATCGA+AGG + chr8.1:1566426-1566445 None:intergenic 30.0%
! AACTAGTTCTTCACTTATAC+TGG - chr8.1:1566912-1566931 MS.gene012669:intron 30.0%
! AATTTTCAGTAACTCTGACT+TGG - chr8.1:1564776-1564795 MS.gene012669:CDS 30.0%
! ACTTATTCGTGTATTTGTTG+GGG - chr8.1:1565623-1565642 MS.gene012669:intron 30.0%
! ATGTGTTTCAAGTAAAAGCT+CGG + chr8.1:1566307-1566326 None:intergenic 30.0%
! ATTTTCAGTAACTCTGACTT+GGG - chr8.1:1564777-1564796 MS.gene012669:CDS 30.0%
! CTTCTAACTAGCTTAAACTA+TGG + chr8.1:1564304-1564323 None:intergenic 30.0%
! GAAGTGTTTATAGATATCCA+AGG - chr8.1:1567756-1567775 MS.gene012669:CDS 30.0%
! TAGTCTTTTGATGAATGACA+CGG - chr8.1:1567460-1567479 MS.gene012669:intron 30.0%
! TCTCAGATTTGAAATTGACA+TGG - chr8.1:1566754-1566773 MS.gene012669:intron 30.0%
!! AAACGACACTCATTTTGAAA+CGG - chr8.1:1565977-1565996 MS.gene012669:intron 30.0%
!!! AGGAAGTAATGGATACATTT+TGG - chr8.1:1565055-1565074 MS.gene012669:intron 30.0%
!!! CAAGTATTTTGAAGCTTTCA+TGG + chr8.1:1566726-1566745 None:intergenic 30.0%
!!! TCCAAAGACTGTAGTTTTTT+AGG + chr8.1:1566142-1566161 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + chr8.1:1565976-1565995 None:intergenic 30.0%
AAAACTACAGTCTTTGGACT+TGG - chr8.1:1566144-1566163 MS.gene012669:intron 35.0%
AACGCCAAACGTTTAATATG+AGG + chr8.1:1567409-1567428 None:intergenic 35.0%
AACGTGAACCAAAAGATTTG+TGG - chr8.1:1564823-1564842 MS.gene012669:CDS 35.0%
ACAACTTGACTCCTGATATT+CGG - chr8.1:1566101-1566120 MS.gene012669:intron 35.0%
AGAAGGAAGATGATCTGATT+CGG + chr8.1:1563807-1563826 None:intergenic 35.0%
AGAGAACTTACTTTCTTCAG+CGG - chr8.1:1567228-1567247 MS.gene012669:intron 35.0%
ATATTTACCTGCAGCTCAAA+TGG + chr8.1:1566403-1566422 None:intergenic 35.0%
CAAAGTTGAATTGAGTAACC+GGG + chr8.1:1565267-1565286 None:intergenic 35.0%
CACATACATTCTTTACACCT+TGG + chr8.1:1567776-1567795 None:intergenic 35.0%
CATGTTCTGATCAGCAAAAT+TGG - chr8.1:1564880-1564899 MS.gene012669:CDS 35.0%
CATTCCTTATGTGAATTTGG+GGG - chr8.1:1566782-1566801 MS.gene012669:intron 35.0%
CCAAAGTTGAATTGAGTAAC+CGG + chr8.1:1565268-1565287 None:intergenic 35.0%
CTATAAACACTTCTTGCCTT+AGG + chr8.1:1567749-1567768 None:intergenic 35.0%
CTGTAACAAGGAGCTTAATT+AGG + chr8.1:1567336-1567355 None:intergenic 35.0%
CTTAAATGATTGGCGTCATT+TGG - chr8.1:1565198-1565217 MS.gene012669:CDS 35.0%
GACAACACTTCTGAATCATT+TGG - chr8.1:1565133-1565152 MS.gene012669:CDS 35.0%
GACACTTGTGAGATTTCTTA+TGG - chr8.1:1564103-1564122 MS.gene012669:intron 35.0%
GCTCATTCCTTATGTGAATT+TGG - chr8.1:1566779-1566798 MS.gene012669:intron 35.0%
GTGGAGAAATTGATTACTAG+CGG - chr8.1:1565448-1565467 MS.gene012669:intron 35.0%
GTTAGTACTCTTACTCAGTA+TGG + chr8.1:1565004-1565023 None:intergenic 35.0%
TCTAGATGAAGTCCTCAATT+AGG + chr8.1:1566532-1566551 None:intergenic 35.0%
TGCTCCTCATATTAAACGTT+TGG - chr8.1:1567402-1567421 MS.gene012669:intron 35.0%
TTAAACGTTTGGCGTTATTG+AGG - chr8.1:1567413-1567432 MS.gene012669:intron 35.0%
! AATTAGCTTACTACAAGGGA+AGG + chr8.1:1566198-1566217 None:intergenic 35.0%
! ACACAACAGACTTGTTCAAA+AGG + chr8.1:1564569-1564588 None:intergenic 35.0%
! ACATGAAAAGTGCACAAAAC+AGG + chr8.1:1567364-1567383 None:intergenic 35.0%
! ACTTTGCTATGGCAAACAAT+TGG + chr8.1:1564752-1564771 None:intergenic 35.0%
! ATTAGCTTACTACAAGGGAA+GGG + chr8.1:1566197-1566216 None:intergenic 35.0%
! CAATTTTGCTGATCAGAACA+TGG + chr8.1:1564882-1564901 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG + chr8.1:1565846-1565865 None:intergenic 35.0%
! TATTCTGGGAACAAATTGCA+GGG + chr8.1:1566582-1566601 None:intergenic 35.0%
! TATTGAGGACCGGATTATTT+TGG - chr8.1:1567428-1567447 MS.gene012669:intron 35.0%
! TGACTTGGGTATTTCACATT+TGG - chr8.1:1564791-1564810 MS.gene012669:CDS 35.0%
! TGTGCAGAGTATTAATTGGA+GGG + chr8.1:1565842-1565861 None:intergenic 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + chr8.1:1565741-1565760 None:intergenic 35.0%
!! GTAGCTGGTTCTAATTTGTA+TGG - chr8.1:1567151-1567170 MS.gene012669:intron 35.0%
!! GTGTTTTTGAACAGAGAATG+AGG - chr8.1:1567687-1567706 MS.gene012669:CDS 35.0%
!! TAATAACAGTAGAGCATTGC+CGG + chr8.1:1566477-1566496 None:intergenic 35.0%
!! TATCAACAATTTTGGGGGAT+GGG - chr8.1:1565553-1565572 MS.gene012669:intron 35.0%
!! TTATCAACAATTTTGGGGGA+TGG - chr8.1:1565552-1565571 MS.gene012669:intron 35.0%
!!! GCCTTTTTTTTTTGCAGCTA+TGG + chr8.1:1564973-1564992 None:intergenic 35.0%
!!! TACATTTTGGTTACCGCAAA+TGG - chr8.1:1565068-1565087 MS.gene012669:intron 35.0%
AAAGCCATGAACTACAGATG+TGG + chr8.1:1565705-1565724 None:intergenic 40.0%
AACAGATGTTGACATGGGAA+AGG - chr8.1:1566273-1566292 MS.gene012669:intron 40.0%
ACAGCAGCACCAAAATAATC+CGG + chr8.1:1567440-1567459 None:intergenic 40.0%
ACCAGCAGTTTCTTGTATTC+TGG + chr8.1:1566597-1566616 None:intergenic 40.0%
AGATGTCTCCGAATTGACTA+TGG + chr8.1:1566060-1566079 None:intergenic 40.0%
AGGAAGTCAGCTTTCATCTT+TGG - chr8.1:1567559-1567578 MS.gene012669:intron 40.0%
ATACCATAACGAGAAGGAGA+AGG + chr8.1:1563824-1563843 None:intergenic 40.0%
ATATACCTCTGCCAATGCAT+GGG + chr8.1:1564686-1564705 None:intergenic 40.0%
ATGGATTCTTCAGGTGTCAA+TGG + chr8.1:1567085-1567104 None:intergenic 40.0%
CAGCAAAATTGGGAACCTAA+TGG - chr8.1:1564891-1564910 MS.gene012669:CDS 40.0%
CAGGTAACAGATGTTGACAT+GGG - chr8.1:1566268-1566287 MS.gene012669:intron 40.0%
CATGATCTGATTCTTTAGCC+CGG - chr8.1:1566455-1566474 MS.gene012669:intron 40.0%
CCAGCAGTTTCTTGTATTCT+GGG + chr8.1:1566596-1566615 None:intergenic 40.0%
CCGGTTACTCAATTCAACTT+TGG - chr8.1:1565265-1565284 MS.gene012669:intron 40.0%
CGAAGAAGAGAGGAAAGAAT+TGG - chr8.1:1566808-1566827 MS.gene012669:intron 40.0%
CGCTTATTTGGACGTATTGT+TGG + chr8.1:1567623-1567642 None:intergenic 40.0%
CTCTCTTTCTATTTCCCTTC+AGG - chr8.1:1563978-1563997 MS.gene012669:CDS 40.0%
CTGAAGGGAAATAGAAAGAG+AGG + chr8.1:1563980-1563999 None:intergenic 40.0%
GAACGAAGTGATGATTTAGG+AGG + chr8.1:1563958-1563977 None:intergenic 40.0%
GAAGGAAGATGATCTGATTC+GGG + chr8.1:1563806-1563825 None:intergenic 40.0%
GAGGAACGAAGTGATGATTT+AGG + chr8.1:1563961-1563980 None:intergenic 40.0%
GCTATGGCAAACAATTGGAA+AGG + chr8.1:1564747-1564766 None:intergenic 40.0%
GCTTAAACTATGGACCTGTT+TGG + chr8.1:1564294-1564313 None:intergenic 40.0%
GGGAAATTATGCAAGGATGT+TGG - chr8.1:1564651-1564670 MS.gene012669:CDS 40.0%
GGTGTAAAGAATGTATGTGC+AGG - chr8.1:1567777-1567796 MS.gene012669:CDS 40.0%
GTAGTGTATGGAGAGATACA+AGG - chr8.1:1565309-1565328 MS.gene012669:intron 40.0%
GTTCAGACACACATACCATT+AGG + chr8.1:1564909-1564928 None:intergenic 40.0%
TATATACCTCTGCCAATGCA+TGG + chr8.1:1564687-1564706 None:intergenic 40.0%
TATTGGATTTGGGAATCGCT+TGG - chr8.1:1566363-1566382 MS.gene012669:intron 40.0%
TCAATTCGGAGACATCTATC+AGG - chr8.1:1566063-1566082 MS.gene012669:intron 40.0%
TCCTGATATTCGGATAGTGA+GGG - chr8.1:1566111-1566130 MS.gene012669:intron 40.0%
TCTATTGTAGTTAGCAGCAC+TGG - chr8.1:1567490-1567509 MS.gene012669:intron 40.0%
TGAACCAAAAGATTTGTGGC+AGG - chr8.1:1564827-1564846 MS.gene012669:CDS 40.0%
TTATGCAAGGATGTTGGCAT+TGG - chr8.1:1564657-1564676 MS.gene012669:CDS 40.0%
TTGATTGATCCCACCATTTG+CGG + chr8.1:1565084-1565103 None:intergenic 40.0%
! AGCAAGTACATAGGCTTTTC+AGG - chr8.1:1564392-1564411 MS.gene012669:CDS 40.0%
! ATGGTGGGATCAATCAACAA+CGG - chr8.1:1565087-1565106 MS.gene012669:intron 40.0%
! CGTGTATTTGTTGGGGAAAA+TGG - chr8.1:1565630-1565649 MS.gene012669:intron 40.0%
! GACTAATTTAGGGTACAAGC+TGG + chr8.1:1567203-1567222 None:intergenic 40.0%
! GTATTCTGGGAACAAATTGC+AGG + chr8.1:1566583-1566602 None:intergenic 40.0%
! GTCTTATTGGACCATTGGAT+CGG - chr8.1:1566648-1566667 MS.gene012669:CDS 40.0%
! GTGTGCAGAGTATTAATTGG+AGG + chr8.1:1565843-1565862 None:intergenic 40.0%
! TTTCAAGTAAAAGCTCGGCT+TGG + chr8.1:1566302-1566321 None:intergenic 40.0%
! TTTTTCATCAATGCCAGTGC+AGG + chr8.1:1566857-1566876 None:intergenic 40.0%
!! AATAACAGTAGAGCATTGCC+GGG + chr8.1:1566476-1566495 None:intergenic 40.0%
!! GCTGGTTCTAATTTGTATGG+AGG - chr8.1:1567154-1567173 MS.gene012669:intron 40.0%
!! TTTTGGTTACCGCAAATGGT+GGG - chr8.1:1565072-1565091 MS.gene012669:intron 40.0%
!!! ATTTTGGTTACCGCAAATGG+TGG - chr8.1:1565071-1565090 MS.gene012669:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr8.1:1565981-1566000 MS.gene012669:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr8.1:1566000-1565981 None:intergenic 40.0%
ACATGTACAGACAAGGCCTA+AGG - chr8.1:1567730-1567749 MS.gene012669:CDS 45.0%
ACCTGCAGCTCAAATGGTAT+TGG + chr8.1:1566397-1566416 None:intergenic 45.0%
ACTACAGTCTTTGGACTTGG+AGG - chr8.1:1566147-1566166 MS.gene012669:intron 45.0%
AGATGTTGACATGGGAAAGG+AGG - chr8.1:1566276-1566295 MS.gene012669:intron 45.0%
ATGGTGTCCAAGAAGAAGCA+GGG + chr8.1:1564863-1564882 None:intergenic 45.0%
ATTCGGAGACATCTATCAGG+AGG - chr8.1:1566066-1566085 MS.gene012669:intron 45.0%
ATTTGTATGGAGGTCGCTCA+CGG - chr8.1:1567164-1567183 MS.gene012669:intron 45.0%
CAATACGTCCAAATAAGCGC+AGG - chr8.1:1567624-1567643 MS.gene012669:intron 45.0%
CCAGGTAACAGATGTTGACA+TGG - chr8.1:1566267-1566286 MS.gene012669:intron 45.0%
CCATGTCAACATCTGTTACC+TGG + chr8.1:1566270-1566289 None:intergenic 45.0%
CCCAGAATACAAGAAACTGC+TGG - chr8.1:1566593-1566612 MS.gene012669:intron 45.0%
CCTTCGATACCATAACGAGA+AGG + chr8.1:1563830-1563849 None:intergenic 45.0%
CGAAGTGATGATTTAGGAGG+TGG + chr8.1:1563955-1563974 None:intergenic 45.0%
CGCAGAAGGGAAATTATGCA+AGG - chr8.1:1564644-1564663 MS.gene012669:CDS 45.0%
CTCCTGATATTCGGATAGTG+AGG - chr8.1:1566110-1566129 MS.gene012669:intron 45.0%
CTGACTTCCTGAATCAGTCA+TGG + chr8.1:1567549-1567568 None:intergenic 45.0%
CTTCCTTCTCCTTCTCGTTA+TGG - chr8.1:1563818-1563837 MS.gene012669:CDS 45.0%
GATGCCACATCTGTAGTTCA+TGG - chr8.1:1565698-1565717 MS.gene012669:intron 45.0%
GGTTTGTAATGCTGTTGTCG+TGG - chr8.1:1565241-1565260 MS.gene012669:intron 45.0%
GTTACTGGGTGAATGATCAG+TGG - chr8.1:1565429-1565448 MS.gene012669:CDS 45.0%
TCAATGCCAGTGCAGGAAAA+TGG + chr8.1:1566850-1566869 None:intergenic 45.0%
TCCAATACCATTTGAGCTGC+AGG - chr8.1:1566393-1566412 MS.gene012669:intron 45.0%
TCCCTCACTATCCGAATATC+AGG + chr8.1:1566115-1566134 None:intergenic 45.0%
TCCTTATGTGAATTTGGGGG+AGG - chr8.1:1566785-1566804 MS.gene012669:intron 45.0%
TCTTTGGACTTGGAGGCAAT+CGG - chr8.1:1566154-1566173 MS.gene012669:intron 45.0%
TGGTGGGATCAATCAACAAC+GGG - chr8.1:1565088-1565107 MS.gene012669:intron 45.0%
TTGCTTCTTAAGAGGTTGCG+CGG - chr8.1:1566617-1566636 MS.gene012669:CDS 45.0%
! AGCATGAGGATGGATTCTTC+AGG + chr8.1:1567094-1567113 None:intergenic 45.0%
! AGTACGTGAATTTGGTGAGC+TGG + chr8.1:1563740-1563759 None:intergenic 45.0%
! CCTTCTCGTTATGGTATCGA+AGG - chr8.1:1563827-1563846 MS.gene012669:CDS 45.0%
! CTACAGATGTGGCATCGAAA+TGG + chr8.1:1565694-1565713 None:intergenic 45.0%
! GCATTTGCCATGACTGATTC+AGG - chr8.1:1567539-1567558 MS.gene012669:intron 45.0%
! GGTTCCTGCCACAAATCTTT+TGG + chr8.1:1564834-1564853 None:intergenic 45.0%
! GTGAATTTGGTGAGCTGGAA+CGG + chr8.1:1563735-1563754 None:intergenic 45.0%
! TGAGATCCATTTTCCTGCAC+TGG - chr8.1:1566841-1566860 MS.gene012669:CDS 45.0%
! TGGTTCGGAGTACGTGAATT+TGG + chr8.1:1563748-1563767 None:intergenic 45.0%
! TTAGCAGCACTGGACTTCAT+AGG - chr8.1:1567500-1567519 MS.gene012669:intron 45.0%
!! CAGCTTTCATCTTTGGTGTC+TGG - chr8.1:1567566-1567585 MS.gene012669:intron 45.0%
!! CTGCTGGTTTGCTTCTTAAG+AGG - chr8.1:1566609-1566628 MS.gene012669:intron 45.0%
!! TGCAGGTCTTATTGGACCAT+TGG - chr8.1:1566643-1566662 MS.gene012669:CDS 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr8.1:1565980-1565999 MS.gene012669:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr8.1:1565999-1565980 None:intergenic 45.0%
AAGAGCAGCAAGCATGAGGA+TGG + chr8.1:1567104-1567123 None:intergenic 50.0%
AGATTTGTGGCAGGAACCCT+TGG - chr8.1:1564836-1564855 MS.gene012669:CDS 50.0%
AGTCATGGCAAATGCATCCG+AGG + chr8.1:1567534-1567553 None:intergenic 50.0%
CAAGAAGAAGCAGGGACCAA+GGG + chr8.1:1564855-1564874 None:intergenic 50.0%
CATGGTGTCCAAGAAGAAGC+AGG + chr8.1:1564864-1564883 None:intergenic 50.0%
CCAACAAGATGCCGATCCAA+TGG + chr8.1:1566662-1566681 None:intergenic 50.0%
CCATTGGATCGGCATCTTGT+TGG - chr8.1:1566659-1566678 MS.gene012669:CDS 50.0%
CGGCATCTTGTTGGTCCATT+TGG - chr8.1:1566668-1566687 MS.gene012669:CDS 50.0%
CGTTTGGCGTTATTGAGGAC+CGG - chr8.1:1567418-1567437 MS.gene012669:intron 50.0%
CTAGCAAGCCTGCGCTTATT+TGG + chr8.1:1567635-1567654 None:intergenic 50.0%
CTTCAGAGCTCAGGTCTGAA+GGG - chr8.1:1567053-1567072 MS.gene012669:intron 50.0%
GCCTCCCCCAAATTCACATA+AGG + chr8.1:1566789-1566808 None:intergenic 50.0%
GGCTGATTGTGAGTTGCAAC+AGG - chr8.1:1565220-1565239 MS.gene012669:CDS 50.0%
TACCTTCTCCTTCAGAGCTC+AGG - chr8.1:1567044-1567063 MS.gene012669:intron 50.0%
TGCTTCTTAAGAGGTTGCGC+GGG - chr8.1:1566618-1566637 MS.gene012669:CDS 50.0%
! ATCCTCATGCTTGCTGCTCT+TGG - chr8.1:1567103-1567122 MS.gene012669:intron 50.0%
!! AGATGATCTGATTCGGGAGC+TGG + chr8.1:1563800-1563819 None:intergenic 50.0%
AGCCAAGAGCAGCAAGCATG+AGG + chr8.1:1567108-1567127 None:intergenic 55.0%
CCAAGAAGAAGCAGGGACCA+AGG + chr8.1:1564856-1564875 None:intergenic 55.0%
CCGAACCATTCTCCGAGACT+CGG - chr8.1:1563760-1563779 MS.gene012669:CDS 55.0%
CCGAGTCTCGGAGAATGGTT+CGG + chr8.1:1563763-1563782 None:intergenic 55.0%
CCTTCAGAGCTCAGGTCTGA+AGG - chr8.1:1567052-1567071 MS.gene012669:intron 55.0%
CCTTGGTCCCTGCTTCTTCT+TGG - chr8.1:1564853-1564872 MS.gene012669:CDS 55.0%
CTTCATAGGCTGTGCTGCCT+CGG - chr8.1:1567514-1567533 MS.gene012669:intron 55.0%
GTAATGCTGTTGTCGTGGCC+CGG - chr8.1:1565246-1565265 MS.gene012669:intron 55.0%
! CCTTCAGACCTGAGCTCTGA+AGG + chr8.1:1567055-1567074 None:intergenic 55.0%
!! GACCTGAGCTCTGAAGGAGA+AGG + chr8.1:1567049-1567068 None:intergenic 55.0%
GTATGGAGGTCGCTCACGGT+TGG - chr8.1:1567168-1567187 MS.gene012669:intron 60.0%
TCGGACCGAGTCTCGGAGAA+TGG + chr8.1:1563768-1563787 None:intergenic 60.0%
TTGGGGGAGGCGAAGAAGAG+AGG - chr8.1:1566798-1566817 MS.gene012669:intron 60.0%
! CGCGGGCATGCAGGTCTTAT+TGG - chr8.1:1566635-1566654 MS.gene012669:CDS 60.0%
! TGATTCGGGAGCTGGAGAGC+CGG + chr8.1:1563792-1563811 None:intergenic 60.0%
!! GGCATTGGCTGCCCATGCAT+TGG - chr8.1:1564672-1564691 MS.gene012669:CDS 60.0%
AAGAGGTTGCGCGGGCATGC+AGG - chr8.1:1566626-1566645 MS.gene012669:CDS 65.0%
! GGCTGCCCATGCATTGGCAG+AGG - chr8.1:1564678-1564697 MS.gene012669:CDS 65.0%
! CTCCGAGACTCGGTCCGAGC+CGG - chr8.1:1563770-1563789 MS.gene012669:CDS 70.0%
!! AGCCGGCTCGGACCGAGTCT+CGG + chr8.1:1563775-1563794 None:intergenic 70.0%
CGGGAGCTGGAGAGCCGGCT+CGG + chr8.1:1563787-1563806 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 1563715 1567808 1563715 ID=MS.gene012669
chr8.1 mRNA 1563715 1567808 1563715 ID=MS.gene012669.t1;Parent=MS.gene012669
chr8.1 exon 1567662 1567808 1567662 ID=MS.gene012669.t1.exon1;Parent=MS.gene012669.t1
chr8.1 CDS 1567662 1567808 1567662 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1566824 1566913 1566824 ID=MS.gene012669.t1.exon2;Parent=MS.gene012669.t1
chr8.1 CDS 1566824 1566913 1566824 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1566611 1566704 1566611 ID=MS.gene012669.t1.exon3;Parent=MS.gene012669.t1
chr8.1 CDS 1566611 1566704 1566611 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1566408 1566469 1566408 ID=MS.gene012669.t1.exon4;Parent=MS.gene012669.t1
chr8.1 CDS 1566408 1566469 1566408 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1566193 1566281 1566193 ID=MS.gene012669.t1.exon5;Parent=MS.gene012669.t1
chr8.1 CDS 1566193 1566281 1566193 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1565340 1565460 1565340 ID=MS.gene012669.t1.exon6;Parent=MS.gene012669.t1
chr8.1 CDS 1565340 1565460 1565340 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1565109 1565252 1565109 ID=MS.gene012669.t1.exon7;Parent=MS.gene012669.t1
chr8.1 CDS 1565109 1565252 1565109 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1564638 1564972 1564638 ID=MS.gene012669.t1.exon8;Parent=MS.gene012669.t1
chr8.1 CDS 1564638 1564972 1564638 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1564241 1564481 1564241 ID=MS.gene012669.t1.exon9;Parent=MS.gene012669.t1
chr8.1 CDS 1564241 1564481 1564241 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
chr8.1 exon 1563715 1564023 1563715 ID=MS.gene012669.t1.exon10;Parent=MS.gene012669.t1
chr8.1 CDS 1563715 1564023 1563715 ID=cds.MS.gene012669.t1;Parent=MS.gene012669.t1
Gene Sequence

>MS.gene012669

ATGGACGTAGATCGGAACCGTTCCAGCTCACCAAATTCACGTACTCCGAACCATTCTCCGAGACTCGGTCCGAGCCGGCTCTCCAGCTCCCGAATCAGATCATCTTCCTTCTCCTTCTCGTTATGGTATCGAAGGAAACGAGCGAAAGTGATTTTAGCACTTATTGCGTTACTCGCTTTATTTTTCTTTGTGAATTATGTTATGCTTCTTCGTCTTCAACATCAACAACAACAAGATCCACCTCCTAAATCATCACTTCGTTCCTCTCTTTCTATTTCCCTTCAGGTTTCAATTCTCATATATTAATTAATTAATATTCGCATTTCATTTTTCATTCCTATCTATCTATAGAAATTATTTAGTACCTTAGATTGTTTATTACGACATAGACACTTGTGAGATTTCTTATGGAAACTGCTTATTATTTCAACTTATATATAGTTAGATATTAATTATAATTTGTTTAATAGTTTAACTTGTTTGGAAAACTTATGAAAATAAGCTATAAACAATGCCGTAAATTGTTACTTACTGTCATAAGTTCTTATGGCAGTAGATAAGCTCAAATAAGTCAATCCAAACAGGTCCATAGTTTAAGCTAGTTAGAAGTAATTCTAATTCTAACTACCTTGCTTGAACTATATTAAACTAATTCTAATTAATATCTAACTATATCAAGCAAGTACATAGGCTTTTCAGGATTACTTATGAAAATAGCTTATATGAAAACAGCTTGACTTTATTTTATACCTTGTTATAGAAATAGCTTATAGACAAGCAGTTATATGATAACCGATTACACTATAAGCATTTAATTAAGTTGTTTGTTGAAATTGTGCCTATATCTGTTTCCTTTTGAACAAGTCTGTTGTGTTTCTGATATTTATTTTTTCAGGGAAAATCGAACAAGAGTAGTACTAAGAAAAAAACGCAGAAGGGAAATTATGCAAGGATGTTGGCATTGGCTGCCCATGCATTGGCAGAGGTATATATTTTGAAGAAAATGTGCCTAGTTTGTAGTTATATAAGCCTTTCCAATTGTTTGCCATAGCAAAGTTTTCAATTTTCAGTAACTCTGACTTGGGTATTTCACATTTGGTACAGAACAAACGTGAACCAAAAGATTTGTGGCAGGAACCCTTGGTCCCTGCTTCTTCTTGGACACCATGTTCTGATCAGCAAAATTGGGAACCTAATGGTATGTGTGTCTGAACTGAACTTCAAAATTTGACAATTGTTTGTATTATATATCTGTCCATAGCTGCAAAAAAAAAAGGCTTATTAGTCCATACTGAGTAAGAGTACTAACAGTTTGCTCTCTTCTACTCTACTACAATAGAAGGAAGTAATGGATACATTTTGGTTACCGCAAATGGTGGGATCAATCAACAACGGGTAGCTGTAAGTATTCTTTATCGGACAACACTTCTGAATCATTTGGGATTAACTTATTTGAAATGAGAAAAAAATTAATAAGCTTTATCTTAAATGATTGGCGTCATTTGGGCTGATTGTGAGTTGCAACAGGTTTGTAATGCTGTTGTCGTGGCCCGGTTACTCAATTCAACTTTGGTTATCCCAAAATTTATGTACAGTAGTGTATGGAGAGATACAAGGTAACACTATGTCCATTTCTCTCTCATTGTCATTACTCACATAATTACTCTGTTTTGTTTTCCCTATGTACTTTTTATTTGATGAAACCTAATATTTAGTTACTGGGTGAATGATCAGTGGAGAAATTGATTACTAGCGGCATGTTATAGTATGCGTGTGAAATTTGATCAACCAGAATTGGTTGAATTAAATTTATTATTCAGATAAATAGTTATAAGATTTATCAACAATTTTGGGGGATGGGTTGAATATACGACTTTATACTTTACCACTTCTAGCATTCTTGTTTTAACTTATTCGTGTATTTGTTGGGGAAAATGGCTTATTTGAATCTCTAGTTTTAGCTTTTTCTTGTTGTACCATTTCGATGCCACATCTGTAGTTCATGGCTTTTCTATTACTCCCTCCGTTTCAAAATGAGTGTCGCTTTAGCCAAAAAAAATTTGTTTCAAAATGAGTGTCGCTTTCAGTTTCCAATGCAATAATAACTTTTTCTTTTCAATTGTACCCTCCAATTAATACTCTGCACACTACTTCCAAAGTATTACTTTTAAAACTAACCAATAGTTAATAAGGATAATTTGGTAAAATAACCATTATCTTTCTCTTAATCATTGCATTTCTTAATATGTGTGTAAACACCTAAAACGACACTCATTTTGAAACGGAGGGAGTATGATTTTGTGACCGTCAATTTAACTTATAAATTTTTATATTGTATAATTCCATAGTCAATTCGGAGACATCTATCAGGAGGAGCATTTTATTAACAACTTGACTCCTGATATTCGGATAGTGAGGGAGCTTCCTAAAAAACTACAGTCTTTGGACTTGGAGGCAATCGGCAGTGTTGTAAGTCACTCCCTTCCCTTGTAGTAAGCTAATTAAACTCAACGATATGAGTCCTATTTGTATTTAATTGTTAGAAGACTTTACCAGGTAACAGATGTTGACATGGGAAAGGAGGCCAAGCCGAGCTTTTACTTGAAACACATCCTGCCTATTATACTTAAAAATCAAGTTGTTCACTTTATTGGATTTGGGAATCGCTTGGCATTTGATCCAATACCATTTGAGCTGCAGGTAAATATCCTTCGATAATTAGACATCACAATCTAATTTTCATGATCTGATTCTTTAGCCCGGCAATGCTCTACTGTTATTAACTTTCTTAATCTCAAGCCCTATTATGCTTTTCCTAATTGAGGACTTCATCTAGAGACTTCGTTGCAGATGTAATTTTCACGCCCTGCAATTTGTTCCCAGAATACAAGAAACTGCTGGTTTGCTTCTTAAGAGGTTGCGCGGGCATGCAGGTCTTATTGGACCATTGGATCGGCATCTTGTTGGTCCATTTGGAGAATTAAATTTGGAGAAGAGTGAAAAAAGTTCCATGAAAGCTTCAAAATACTTGGCTTTACATCTCAGATTTGAAATTGACATGGTAGCTCATTCCTTATGTGAATTTGGGGGAGGCGAAGAAGAGAGGAAAGAATTGGAAGCATATCGTGAGATCCATTTTCCTGCACTGGCATTGATGAAAAATACTACTAAGTATGTATCACAATTTTTTTTATCTCAACTAGTTCTTCACTTATACTGGAAACACTTGTACTCGTCTTCTTTTAAACACAATAATTTTAATTTGTGATGAAAAATGTTTTGCTCTTTAGCTACTTTGATTTAAAGTAATTGGAGTTTTCTGTGCAGATTACCTTCTCCTTCAGAGCTCAGGTCTGAAGGGCTATGTCCATTGACACCTGAAGAATCCATCCTCATGCTTGCTGCTCTTGGCTTTAACCGCAAGACAAACATATTTGTAGCTGGTTCTAATTTGTATGGAGGTCGCTCACGGTTGGCTGCTTTGACCAGCTTGTACCCTAAATTAGTCACTAAAGAGAACTTACTTTCTTCAGCGGAACTCGAACCATTTGCTAATTATTCATCTCAGGTTTGCTGCTACAGTCATTCTTGTTTCATTTATTTCAATGATGTCACAAACCTAATTAAGCTCCTTGTTACAGTAAATCCTGTTTTGTGCACTTTTCATGTTCTATATGTTCTATAAATTGCTCCTCATATTAAACGTTTGGCGTTATTGAGGACCGGATTATTTTGGTGCTGCTGTTAGTCTTTTGATGAATGACACGGAATTATTTCTATTGTAGTTAGCAGCACTGGACTTCATAGGCTGTGCTGCCTCGGATGCATTTGCCATGACTGATTCAGGAAGTCAGCTTTCATCTTTGGTGTCTGGTTATCGAATATATTATGGTGGAGGAAGAATGCCAACAATACGTCCAAATAAGCGCAGGCTTGCTAGTATATTCATGAAAAACACTACAATAGAATGGCGTGTTTTTGAACAGAGAATGAGGAAAGCTGTCAGACAAACTAAACATGTACAGACAAGGCCTAAGGCAAGAAGTGTTTATAGATATCCAAGGTGTAAAGAATGTATGTGCAGGACAATTTAA
Protein sequence

>MS.gene012669.t1

MDVDRNRSSSPNSRTPNHSPRLGPSRLSSSRIRSSSFSFSLWYRRKRAKGKSNKSSTKKKTQKGNYARMLALAAHALAENKREPKDLWQEPLVPASSWTPCSDQQNWEPNEGSNGYILVTANGGINQQRVAVCNAVVVARLLNSTLVIPKFMYSSVWRDTSQFGDIYQEEHFINNLTPDIRIVRELPKKLQSLDLEAIGSVVTDVDMGKEAKPSFYLKHILPIILKNQVVHFIGFGNRLAFDPIPFELQRLRCRCNFHALQFVPRIQETAGLLLKRLRGHAGLIGPLDRHLVGPFGELNLEKSEKSSMKASKYLALHLRFEIDMVAHSLCEFGGGEEERKELEAYREIHFPALALMKNTTKLPSPSELRSEGLCPLTPEESILMLAALGFNRKTNIFVAGSNLYGGRSRLAALTSLYPKLVTKENLLSSAELEPFANYSSQLAALDFIGCAASDAFAMTDSGSQLSSLVSGYRIYYGGGRMPTIRPNKRRLASIFMKNTTIEWRVFEQRMRKAVRQTKHVQTRPKARSVYRYPRCKECMCRTI