Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012735.t1 | PNY14930.1 | 94.4 | 124 | 7 | 0 | 1 | 124 | 1 | 124 | 1.50E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012735.t1 | Q9FGJ4 | 55.9 | 127 | 52 | 1 | 1 | 123 | 1 | 127 | 7.3e-30 | 131.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012735.t1 | A0A2K3PI09 | 94.4 | 124 | 7 | 0 | 1 | 124 | 1 | 124 | 1.1e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012735 | MS.gene72802 | PPI |
| MS.gene52597 | MS.gene012735 | PPI |
| MS.gene012735 | MS.gene033495 | PPI |
| MS.gene012735 | MS.gene033497 | PPI |
| MS.gene012735 | MS.gene82126 | PPI |
| MS.gene012735 | MS.gene024056 | PPI |
| MS.gene012735 | MS.gene058580 | PPI |
| MS.gene012735 | MS.gene88356 | PPI |
| MS.gene012735 | MS.gene028119 | PPI |
| MS.gene064261 | MS.gene012735 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012735.t1 | MTR_4g133928 | 93.548 | 124 | 8 | 0 | 1 | 124 | 1 | 124 | 2.38e-82 | 237 |
| MS.gene012735.t1 | MTR_0446s0010 | 87.963 | 108 | 10 | 1 | 1 | 108 | 1 | 105 | 2.30e-64 | 191 |
| MS.gene012735.t1 | MTR_7g080760 | 47.297 | 74 | 39 | 0 | 47 | 120 | 43 | 116 | 7.99e-21 | 81.3 |
| MS.gene012735.t1 | MTR_7g080390 | 49.351 | 77 | 39 | 0 | 47 | 123 | 54 | 130 | 9.22e-21 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012735.t1 | AT5G47630 | 55.906 | 127 | 52 | 1 | 1 | 123 | 1 | 127 | 3.96e-42 | 135 |
| MS.gene012735.t1 | AT5G47630 | 55.906 | 127 | 52 | 1 | 1 | 123 | 1 | 127 | 3.96e-42 | 135 |
| MS.gene012735.t1 | AT5G47630 | 55.906 | 127 | 52 | 1 | 1 | 123 | 1 | 127 | 3.96e-42 | 135 |
| MS.gene012735.t1 | AT1G65290 | 49.351 | 77 | 39 | 0 | 47 | 123 | 50 | 126 | 1.34e-20 | 80.9 |
| MS.gene012735.t1 | AT2G44620 | 40.000 | 100 | 47 | 1 | 17 | 116 | 29 | 115 | 2.15e-20 | 80.5 |
Find 18 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAGGGTTGAGCTTATTA+TGG | 0.307345 | 8.1:-768052 | MS.gene012735:CDS |
| AGCTTATCTGCTTTCTCATC+AGG | 0.361227 | 8.1:+768000 | None:intergenic |
| CATAATCACATTATCATCCT+TGG | 0.439969 | 8.1:+768346 | None:intergenic |
| GGACTTAAACCTGGACAGCT+TGG | 0.459469 | 8.1:-768073 | MS.gene012735:CDS |
| TGATTATGCATTTGAGATGT+TGG | 0.460487 | 8.1:-768331 | MS.gene012735:CDS |
| TGAGAAGGTCAACTGAAAGA+TGG | 0.475010 | 8.1:-768376 | MS.gene012735:CDS |
| GTCTAGTATCGGATCAGAGC+TGG | 0.475971 | 8.1:+768292 | None:intergenic |
| AGATGTTGGTGCTCTTCAGC+AGG | 0.487417 | 8.1:-768317 | MS.gene012735:CDS |
| CAACCCTATCCAAGCTGTCC+AGG | 0.498453 | 8.1:+768064 | None:intergenic |
| TAAACCTGGACAGCTTGGAT+AGG | 0.517739 | 8.1:-768068 | MS.gene012735:CDS |
| AAACCTGGACAGCTTGGATA+GGG | 0.522123 | 8.1:-768067 | MS.gene012735:CDS |
| CTGATTACTCGGTCTAGTAT+CGG | 0.536163 | 8.1:+768281 | None:intergenic |
| GGCTATGTACTTTGCAACAT+CGG | 0.551029 | 8.1:+767966 | None:intergenic |
| TCTGGACCAGTCTGATTACT+CGG | 0.560515 | 8.1:+768270 | None:intergenic |
| TTTCTCATCAGGAATTTCGA+TGG | 0.580152 | 8.1:+768011 | None:intergenic |
| TGATAAAATTAATGCAGCAA+AGG | 0.580568 | 8.1:-768243 | MS.gene012735:intron |
| TAAGTCATGTGAATTTGAGA+AGG | 0.613186 | 8.1:-768391 | MS.gene012735:CDS |
| ACTAGACCGAGTAATCAGAC+TGG | 0.680991 | 8.1:-768276 | MS.gene012735:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTAATTTTATCATATTTC+TGG | + | chr8.1:768109-768128 | None:intergenic | 15.0% |
| !!! | ATTGGTATATATTTTTGTGT+AGG | - | chr8.1:768237-768256 | MS.gene012735:intron | 20.0% |
| !!! | TATTTTCTTTTGACTATAAC+TGG | + | chr8.1:768145-768164 | None:intergenic | 20.0% |
| ! | TGATAAAATTAATGCAGCAA+AGG | - | chr8.1:768115-768134 | MS.gene012735:intron | 25.0% |
| CATAATCACATTATCATCCT+TGG | + | chr8.1:768015-768034 | None:intergenic | 30.0% | |
| TAAGTCATGTGAATTTGAGA+AGG | - | chr8.1:767967-767986 | MS.gene012735:CDS | 30.0% | |
| TTCACGTGTAGATTAAGTAT+TGG | - | chr8.1:768219-768238 | MS.gene012735:intron | 30.0% | |
| ! | TGATTATGCATTTGAGATGT+TGG | - | chr8.1:768027-768046 | MS.gene012735:CDS | 30.0% |
| ! | TTTTCAAAAGGACTTAAACC+TGG | - | chr8.1:768276-768295 | MS.gene012735:CDS | 30.0% |
| TTATCAGTGTTACACAGTCT+GGG | - | chr8.1:768188-768207 | MS.gene012735:intron | 35.0% | |
| TTTATCAGTGTTACACAGTC+TGG | - | chr8.1:768187-768206 | MS.gene012735:intron | 35.0% | |
| TTTCTCATCAGGAATTTCGA+TGG | + | chr8.1:768350-768369 | None:intergenic | 35.0% | |
| ! | GTCAACTGAAAGATGGTTTT+TGG | - | chr8.1:767989-768008 | MS.gene012735:CDS | 35.0% |
| !! | TGAAGCAGCTGATTTTCAAA+AGG | - | chr8.1:768264-768283 | MS.gene012735:CDS | 35.0% |
| !!! | AATTTTTTGGTCAGCTTCAG+AGG | + | chr8.1:768416-768435 | None:intergenic | 35.0% |
| AGCTTATCTGCTTTCTCATC+AGG | + | chr8.1:768361-768380 | None:intergenic | 40.0% | |
| CTGATTACTCGGTCTAGTAT+CGG | + | chr8.1:768080-768099 | None:intergenic | 40.0% | |
| GGATAGGGTTGAGCTTATTA+TGG | - | chr8.1:768306-768325 | MS.gene012735:CDS | 40.0% | |
| GGCTATGTACTTTGCAACAT+CGG | + | chr8.1:768395-768414 | None:intergenic | 40.0% | |
| TGAGAAGGTCAACTGAAAGA+TGG | - | chr8.1:767982-768001 | MS.gene012735:CDS | 40.0% | |
| !!! | TGAAAGATGGTTTTTGGCCA+AGG | - | chr8.1:767995-768014 | MS.gene012735:CDS | 40.0% |
| AAACCTGGACAGCTTGGATA+GGG | - | chr8.1:768291-768310 | MS.gene012735:CDS | 45.0% | |
| ACTAGACCGAGTAATCAGAC+TGG | - | chr8.1:768082-768101 | MS.gene012735:CDS | 45.0% | |
| TAAACCTGGACAGCTTGGAT+AGG | - | chr8.1:768290-768309 | MS.gene012735:CDS | 45.0% | |
| TCTGGACCAGTCTGATTACT+CGG | + | chr8.1:768091-768110 | None:intergenic | 45.0% | |
| GGACTTAAACCTGGACAGCT+TGG | - | chr8.1:768285-768304 | MS.gene012735:CDS | 50.0% | |
| ! | AGATGTTGGTGCTCTTCAGC+AGG | - | chr8.1:768041-768060 | MS.gene012735:CDS | 50.0% |
| ! | GTCTAGTATCGGATCAGAGC+TGG | + | chr8.1:768069-768088 | None:intergenic | 50.0% |
| CAACCCTATCCAAGCTGTCC+AGG | + | chr8.1:768297-768316 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 767942 | 768438 | 767942 | ID=MS.gene012735 |
| chr8.1 | mRNA | 767942 | 768438 | 767942 | ID=MS.gene012735.t1;Parent=MS.gene012735 |
| chr8.1 | exon | 768244 | 768438 | 768244 | ID=MS.gene012735.t1.exon1;Parent=MS.gene012735.t1 |
| chr8.1 | CDS | 768244 | 768438 | 768244 | ID=cds.MS.gene012735.t1;Parent=MS.gene012735.t1 |
| chr8.1 | exon | 767942 | 768121 | 767942 | ID=MS.gene012735.t1.exon2;Parent=MS.gene012735.t1 |
| chr8.1 | CDS | 767942 | 768121 | 767942 | ID=cds.MS.gene012735.t1;Parent=MS.gene012735.t1 |
| Gene Sequence |
| Protein sequence |
