Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064261.t1 | XP_003624206.1 | 98.5 | 130 | 2 | 0 | 1 | 130 | 1 | 130 | 4.50E-66 | 260.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064261.t1 | O80800 | 72.9 | 133 | 26 | 3 | 1 | 130 | 1 | 126 | 4.2e-44 | 178.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064261.t1 | B7FLI7 | 98.5 | 130 | 2 | 0 | 1 | 130 | 1 | 130 | 3.3e-66 | 260.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene064261 | MS.gene95103 | PPI |
| MS.gene064261 | MS.gene43422 | PPI |
| MS.gene064261 | MS.gene042091 | PPI |
| MS.gene064261 | MS.gene012735 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064261.t1 | MTR_7g080390 | 98.462 | 130 | 2 | 0 | 1 | 130 | 1 | 130 | 7.91e-93 | 264 |
| MS.gene064261.t1 | MTR_7g080760 | 51.240 | 121 | 49 | 3 | 10 | 129 | 7 | 118 | 2.50e-35 | 118 |
| MS.gene064261.t1 | MTR_4g133928 | 51.948 | 77 | 37 | 0 | 54 | 130 | 47 | 123 | 1.99e-21 | 83.2 |
| MS.gene064261.t1 | MTR_0446s0010 | 58.929 | 56 | 23 | 0 | 54 | 109 | 47 | 102 | 4.89e-16 | 68.9 |
| MS.gene064261.t1 | MTR_8g099920 | 41.772 | 79 | 44 | 2 | 53 | 130 | 59 | 136 | 2.70e-11 | 57.4 |
| MS.gene064261.t1 | MTR_8g099920 | 41.772 | 79 | 44 | 2 | 53 | 130 | 61 | 138 | 2.81e-11 | 57.4 |
| MS.gene064261.t1 | MTR_4g118750 | 35.398 | 113 | 67 | 3 | 16 | 123 | 4 | 115 | 5.81e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064261.t1 | AT1G65290 | 72.932 | 133 | 26 | 3 | 1 | 130 | 1 | 126 | 4.19e-60 | 181 |
| MS.gene064261.t1 | AT2G44620 | 52.713 | 129 | 53 | 4 | 1 | 129 | 1 | 121 | 1.12e-35 | 119 |
| MS.gene064261.t1 | AT5G47630 | 37.097 | 124 | 71 | 3 | 10 | 130 | 8 | 127 | 8.30e-20 | 79.3 |
| MS.gene064261.t1 | AT5G47630 | 37.097 | 124 | 71 | 3 | 10 | 130 | 8 | 127 | 8.30e-20 | 79.3 |
| MS.gene064261.t1 | AT5G47630 | 37.097 | 124 | 71 | 3 | 10 | 130 | 8 | 127 | 8.30e-20 | 79.3 |
Find 36 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTAGAAGGATCAACTTTC+TGG | 0.153197 | 7.3:-27145792 | None:intergenic |
| ATCTTGTCTGCTTCATTATC+AGG | 0.242778 | 7.3:-27147358 | None:intergenic |
| GAAACGACGAAGGAAGAAAT+TGG | 0.274656 | 7.3:-27145695 | None:intergenic |
| TATCCAATCCAAGGTCATTC+TGG | 0.280875 | 7.3:-27147279 | None:intergenic |
| CCAGAAAGTTGATCCTTCTA+AGG | 0.310928 | 7.3:+27145792 | MS.gene064261:CDS |
| GTCGTTTCAGCGAAGAGGTT+AGG | 0.325470 | 7.3:+27145710 | MS.gene064261:CDS |
| TGACCTTGGATTGGATAGTT+TGG | 0.330609 | 7.3:+27147285 | MS.gene064261:CDS |
| ATGGCTCTTGAGGAAGAGTT+TGG | 0.348440 | 7.3:+27147325 | MS.gene064261:CDS |
| TCGTTTCAGCGAAGAGGTTA+GGG | 0.350830 | 7.3:+27145711 | MS.gene064261:CDS |
| AGGAATGAATATACCTTAGA+AGG | 0.366704 | 7.3:-27145805 | None:intergenic |
| TGGCTCTTGAGGAAGAGTTT+GGG | 0.415460 | 7.3:+27147326 | MS.gene064261:CDS |
| ACAATGGCGGCGAGAAGAAC+CGG | 0.449215 | 7.3:+27145595 | None:intergenic |
| TTTCCAGAATGACCTTGGAT+TGG | 0.459097 | 7.3:+27147276 | MS.gene064261:CDS |
| CTTCATTGCATCTCATCCTC+AGG | 0.467249 | 7.3:+27147405 | MS.gene064261:CDS |
| GTAATTGATCAGAGGTAAAC+CGG | 0.480151 | 7.3:-27145614 | None:intergenic |
| AAACGACGAAGGAAGAAATT+GGG | 0.492698 | 7.3:-27145694 | None:intergenic |
| GCTCATTTCCAGAATGACCT+TGG | 0.503501 | 7.3:+27147271 | MS.gene064261:CDS |
| AAAGGCGATTGGTGGTTCAA+TGG | 0.507901 | 7.3:-27145661 | None:intergenic |
| CTCATCCTCAGGCAAAGTAA+TGG | 0.513408 | 7.3:+27147416 | MS.gene064261:CDS |
| TCATTCTGGAAATGAGCACT+TGG | 0.522843 | 7.3:-27147265 | None:intergenic |
| CCTTCGTCGTTTCAGCGAAG+AGG | 0.534191 | 7.3:+27145705 | MS.gene064261:CDS |
| GAGAATCAACTTGCACTCTC+AGG | 0.548584 | 7.3:-27145636 | None:intergenic |
| CATTCTGGAAATGAGCACTT+GGG | 0.569313 | 7.3:-27147264 | None:intergenic |
| GAAGAAGACGGAAAGGCGAT+TGG | 0.569472 | 7.3:-27145672 | None:intergenic |
| GAAGACGGAAAGGCGATTGG+TGG | 0.580452 | 7.3:-27145669 | None:intergenic |
| GAATTCCATTACTTTGCCTG+AGG | 0.593411 | 7.3:-27147421 | None:intergenic |
| AGAGATTGTGATGGCTCTTG+AGG | 0.598690 | 7.3:+27147315 | MS.gene064261:CDS |
| CGTTTCAGCGAAGAGGTTAG+GGG | 0.604330 | 7.3:+27145712 | MS.gene064261:CDS |
| GGATGCTGTAGAGATTGTGA+TGG | 0.619499 | 7.3:+27147306 | MS.gene064261:CDS |
| AAATTGGGAAGAAGACGGAA+AGG | 0.623328 | 7.3:-27145679 | None:intergenic |
| GGAAGAAATTGGGAAGAAGA+CGG | 0.626548 | 7.3:-27145684 | None:intergenic |
| CATCCAAACTATCCAATCCA+AGG | 0.636485 | 7.3:-27147288 | None:intergenic |
| TACGTTTCTCGACAAATCTG+AGG | 0.640218 | 7.3:+27145735 | MS.gene064261:CDS |
| ATTCTGGAAATGAGCACTTG+GGG | 0.645151 | 7.3:-27147263 | None:intergenic |
| CCTCTTCGCTGAAACGACGA+AGG | 0.663136 | 7.3:-27145705 | None:intergenic |
| ACTCTCAGGTAATTGATCAG+AGG | 0.675792 | 7.3:-27145622 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTATTTCCGATAATT+AGG | + | chr7.3:27145941-27145960 | MS.gene064261:intron | 15.0% |
| !! | AACTAAAACAATGTTATTAT+CGG | - | chr7.3:27146692-27146711 | None:intergenic | 15.0% |
| !! | TACAAAAATATATAAATCCT+TGG | + | chr7.3:27146535-27146554 | MS.gene064261:intron | 15.0% |
| !!! | ATTGTTTTCATATAAGTTAT+AGG | + | chr7.3:27146042-27146061 | MS.gene064261:intron | 15.0% |
| !!! | TTGTTTTCATATAAGTTATA+GGG | + | chr7.3:27146043-27146062 | MS.gene064261:intron | 15.0% |
| !!! | TTTAACTTTATTTTACCTTT+TGG | - | chr7.3:27146153-27146172 | None:intergenic | 15.0% |
| !! | TGATGAATGAAACAATAATT+AGG | - | chr7.3:27145861-27145880 | None:intergenic | 20.0% |
| !! | TTCACTTTATCTTTAGTTAT+AGG | - | chr7.3:27146018-27146037 | None:intergenic | 20.0% |
| !!! | AACATTGTTTTAGTTATATG+AGG | + | chr7.3:27146697-27146716 | MS.gene064261:intron | 20.0% |
| !!! | ATTTTTGTATTGCATTTTAG+TGG | - | chr7.3:27146524-27146543 | None:intergenic | 20.0% |
| !!! | TAAGCTAGATTATTTTCTTA+AGG | - | chr7.3:27146337-27146356 | None:intergenic | 20.0% |
| ! | AAGTGATTACTTCATTTAAG+AGG | + | chr7.3:27146803-27146822 | MS.gene064261:intron | 25.0% |
| ! | AATTGAGTGTAATTCTTACA+TGG | - | chr7.3:27146613-27146632 | None:intergenic | 25.0% |
| ! | ATAAACTTGTGAGACTAATT+GGG | - | chr7.3:27147038-27147057 | None:intergenic | 25.0% |
| ! | ATATAAGAATTGCTCATTGA+TGG | - | chr7.3:27146488-27146507 | None:intergenic | 25.0% |
| ! | ATTAACGTGATGTTATTGTT+AGG | + | chr7.3:27145880-27145899 | MS.gene064261:intron | 25.0% |
| ! | CAATTCTTATATGAAGCTAA+AGG | + | chr7.3:27146496-27146515 | MS.gene064261:intron | 25.0% |
| ! | GAAACGAATGATAATAATAG+AGG | - | chr7.3:27145828-27145847 | None:intergenic | 25.0% |
| ! | GTATATGCTATAAGCTATAT+TGG | + | chr7.3:27146181-27146200 | MS.gene064261:intron | 25.0% |
| ! | TAAGCTACTTATATACCAAA+AGG | + | chr7.3:27146135-27146154 | MS.gene064261:intron | 25.0% |
| ! | TACTTCATTTAAGAGGATTA+AGG | + | chr7.3:27146810-27146829 | MS.gene064261:intron | 25.0% |
| ! | TATAAACTTGTGAGACTAAT+TGG | - | chr7.3:27147039-27147058 | None:intergenic | 25.0% |
| ! | TATAAATCCTTGGATTTGAT+TGG | + | chr7.3:27146545-27146564 | MS.gene064261:intron | 25.0% |
| ! | TCCAAACATTACCTAATTAT+CGG | - | chr7.3:27145955-27145974 | None:intergenic | 25.0% |
| !!! | TAACTTGTTTGAAAACAGTT+TGG | - | chr7.3:27146926-27146945 | None:intergenic | 25.0% |
| AAACATTTCATACGCTACTT+TGG | + | chr7.3:27146308-27146327 | MS.gene064261:intron | 30.0% | |
| AAAGCTGGTAGCAATATTAA+AGG | - | chr7.3:27146459-27146478 | None:intergenic | 30.0% | |
| AAAGTAGCGTATGAAATGTT+TGG | - | chr7.3:27146309-27146328 | None:intergenic | 30.0% | |
| AACATAACCAATCAAATCCA+AGG | - | chr7.3:27146555-27146574 | None:intergenic | 30.0% | |
| AAGAAGTTAGAAACAGCTTA+TGG | + | chr7.3:27146983-27147002 | MS.gene064261:intron | 30.0% | |
| ACAAATAAACCAATCCAAAC+AGG | + | chr7.3:27147068-27147087 | MS.gene064261:intron | 30.0% | |
| ACTTCAAACAGTAAAGTGTA+AGG | - | chr7.3:27147127-27147146 | None:intergenic | 30.0% | |
| AGGAATGAATATACCTTAGA+AGG | - | chr7.3:27145808-27145827 | None:intergenic | 30.0% | |
| CAAATAAACCAATCCAAACA+GGG | + | chr7.3:27147069-27147088 | MS.gene064261:intron | 30.0% | |
| CTTATACTCTCTTGTTAACA+TGG | + | chr7.3:27147210-27147229 | MS.gene064261:intron | 30.0% | |
| TCCGATAATTAGGTAATGTT+TGG | + | chr7.3:27145951-27145970 | MS.gene064261:intron | 30.0% | |
| TTAGGTAATGTTTGGATCAA+CGG | + | chr7.3:27145959-27145978 | MS.gene064261:intron | 30.0% | |
| ! | TTGGATTTGATTGGTTATGT+TGG | + | chr7.3:27146554-27146573 | MS.gene064261:intron | 30.0% |
| !! | TTGAAGTGTGTTTGTCATTT+TGG | + | chr7.3:27147140-27147159 | MS.gene064261:intron | 30.0% |
| AAACGACGAAGGAAGAAATT+GGG | - | chr7.3:27145697-27145716 | None:intergenic | 35.0% | |
| ATCTTGTCTGCTTCATTATC+AGG | - | chr7.3:27147361-27147380 | None:intergenic | 35.0% | |
| CATCTACTCATGTAAGCATT+CGG | - | chr7.3:27146404-27146423 | None:intergenic | 35.0% | |
| CTAGCTTATAGATGTGTCAT+TGG | + | chr7.3:27146349-27146368 | MS.gene064261:intron | 35.0% | |
| CTGATAAATGGAAAGAAGCA+CGG | - | chr7.3:27147239-27147258 | None:intergenic | 35.0% | |
| CTGGTAGCAATATTAAAGGA+AGG | - | chr7.3:27146455-27146474 | None:intergenic | 35.0% | |
| GATGAAATTATCACTGCCAA+GGG | + | chr7.3:27146767-27146786 | MS.gene064261:intron | 35.0% | |
| GTAATTGATCAGAGGTAAAC+CGG | - | chr7.3:27145617-27145636 | None:intergenic | 35.0% | |
| ! | TAAGCTATCTTAGAGAGCAT+AGG | + | chr7.3:27146959-27146978 | MS.gene064261:intron | 35.0% |
| ! | TGATAAATGGAAAGAAGCAC+GGG | - | chr7.3:27147238-27147257 | None:intergenic | 35.0% |
| AAATTGGGAAGAAGACGGAA+AGG | - | chr7.3:27145682-27145701 | None:intergenic | 40.0% | |
| ACATTGTTCAGGAGAGCTTA+TGG | - | chr7.3:27146381-27146400 | None:intergenic | 40.0% | |
| ACTCTCAGGTAATTGATCAG+AGG | - | chr7.3:27145625-27145644 | None:intergenic | 40.0% | |
| ACTTGGGGTAACCTGATAAA+TGG | - | chr7.3:27147251-27147270 | None:intergenic | 40.0% | |
| AGCGTATGAAATGTTTGGCT+TGG | - | chr7.3:27146304-27146323 | None:intergenic | 40.0% | |
| AGCTTATCTACGAGACTCTT+TGG | - | chr7.3:27146273-27146292 | None:intergenic | 40.0% | |
| AGTACACAATTGCTTGCAGA+GGG | - | chr7.3:27147177-27147196 | None:intergenic | 40.0% | |
| CATCCAAACTATCCAATCCA+AGG | - | chr7.3:27147291-27147310 | None:intergenic | 40.0% | |
| CCTTAGAAGGATCAACTTTC+TGG | - | chr7.3:27145795-27145814 | None:intergenic | 40.0% | |
| CGATGAAATTATCACTGCCA+AGG | + | chr7.3:27146766-27146785 | MS.gene064261:intron | 40.0% | |
| CGTGCTTCTTTCCATTTATC+AGG | + | chr7.3:27147237-27147256 | MS.gene064261:intron | 40.0% | |
| CTTAAGAGAGATCAAACCCT+TGG | - | chr7.3:27146786-27146805 | None:intergenic | 40.0% | |
| GAAACGACGAAGGAAGAAAT+TGG | - | chr7.3:27145698-27145717 | None:intergenic | 40.0% | |
| GCTTATCTACGAGACTCTTT+GGG | - | chr7.3:27146272-27146291 | None:intergenic | 40.0% | |
| GGAAGAAATTGGGAAGAAGA+CGG | - | chr7.3:27145687-27145706 | None:intergenic | 40.0% | |
| GTTTACACTATAGCCCTGTT+TGG | - | chr7.3:27147085-27147104 | None:intergenic | 40.0% | |
| TAAGCATTCGGACATTGTTC+AGG | - | chr7.3:27146392-27146411 | None:intergenic | 40.0% | |
| TACGTTTCTCGACAAATCTG+AGG | + | chr7.3:27145735-27145754 | MS.gene064261:CDS | 40.0% | |
| TAGTACACAATTGCTTGCAG+AGG | - | chr7.3:27147178-27147197 | None:intergenic | 40.0% | |
| TATCCAATCCAAGGTCATTC+TGG | - | chr7.3:27147282-27147301 | None:intergenic | 40.0% | |
| TTTCCAGAATGACCTTGGAT+TGG | + | chr7.3:27147276-27147295 | MS.gene064261:CDS | 40.0% | |
| ! | ATTCTGGAAATGAGCACTTG+GGG | - | chr7.3:27147266-27147285 | None:intergenic | 40.0% |
| ! | CATTCTGGAAATGAGCACTT+GGG | - | chr7.3:27147267-27147286 | None:intergenic | 40.0% |
| ! | CCAGAAAGTTGATCCTTCTA+AGG | + | chr7.3:27145792-27145811 | MS.gene064261:CDS | 40.0% |
| ! | TCATTCTGGAAATGAGCACT+TGG | - | chr7.3:27147268-27147287 | None:intergenic | 40.0% |
| ! | TGACCTTGGATTGGATAGTT+TGG | + | chr7.3:27147285-27147304 | MS.gene064261:CDS | 40.0% |
| AGAGATTGTGATGGCTCTTG+AGG | + | chr7.3:27147315-27147334 | MS.gene064261:CDS | 45.0% | |
| ATGGCTCTTGAGGAAGAGTT+TGG | + | chr7.3:27147325-27147344 | MS.gene064261:CDS | 45.0% | |
| CACTATAGCCCTGTTTGGAT+TGG | - | chr7.3:27147080-27147099 | None:intergenic | 45.0% | |
| CTTCATTGCATCTCATCCTC+AGG | + | chr7.3:27147405-27147424 | MS.gene064261:CDS | 45.0% | |
| CTTGTGAGACTAATTGGGAG+AGG | - | chr7.3:27147033-27147052 | None:intergenic | 45.0% | |
| GAGAATCAACTTGCACTCTC+AGG | - | chr7.3:27145639-27145658 | None:intergenic | 45.0% | |
| GCTCATTTCCAGAATGACCT+TGG | + | chr7.3:27147271-27147290 | MS.gene064261:CDS | 45.0% | |
| GGATGCTGTAGAGATTGTGA+TGG | + | chr7.3:27147306-27147325 | MS.gene064261:CDS | 45.0% | |
| TCGTTTCAGCGAAGAGGTTA+GGG | + | chr7.3:27145711-27145730 | MS.gene064261:CDS | 45.0% | |
| TGGCTCTTGAGGAAGAGTTT+GGG | + | chr7.3:27147326-27147345 | MS.gene064261:CDS | 45.0% | |
| ! | AAAGGCGATTGGTGGTTCAA+TGG | - | chr7.3:27145664-27145683 | None:intergenic | 45.0% |
| ! | CATTGATGGCATCGTAAAGC+TGG | - | chr7.3:27146474-27146493 | None:intergenic | 45.0% |
| CGTTTCAGCGAAGAGGTTAG+GGG | + | chr7.3:27145712-27145731 | MS.gene064261:CDS | 50.0% | |
| GAAGAAGACGGAAAGGCGAT+TGG | - | chr7.3:27145675-27145694 | None:intergenic | 50.0% | |
| GTCGTTTCAGCGAAGAGGTT+AGG | + | chr7.3:27145710-27145729 | MS.gene064261:CDS | 50.0% | |
| CCTCTTCGCTGAAACGACGA+AGG | - | chr7.3:27145708-27145727 | None:intergenic | 55.0% | |
| CCTTCGTCGTTTCAGCGAAG+AGG | + | chr7.3:27145705-27145724 | MS.gene064261:CDS | 55.0% | |
| GAAGACGGAAAGGCGATTGG+TGG | - | chr7.3:27145672-27145691 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 27145598 | 27147435 | 27145598 | ID=MS.gene064261 |
| chr7.3 | mRNA | 27145598 | 27147435 | 27145598 | ID=MS.gene064261.t1;Parent=MS.gene064261 |
| chr7.3 | exon | 27145598 | 27145813 | 27145598 | ID=MS.gene064261.t1.exon1;Parent=MS.gene064261.t1 |
| chr7.3 | CDS | 27145598 | 27145813 | 27145598 | ID=cds.MS.gene064261.t1;Parent=MS.gene064261.t1 |
| chr7.3 | exon | 27147259 | 27147435 | 27147259 | ID=MS.gene064261.t1.exon2;Parent=MS.gene064261.t1 |
| chr7.3 | CDS | 27147259 | 27147435 | 27147259 | ID=cds.MS.gene064261.t1;Parent=MS.gene064261.t1 |
| Gene Sequence |
| Protein sequence |
