AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013115


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013115.t1 MTR_3g099680 57.277 213 42 4 1 174 17 219 1.21e-66 203
MS.gene013115.t1 MTR_3g099980 57.346 211 43 4 1 174 17 217 8.82e-66 201
MS.gene013115.t1 MTR_3g099570 65.730 178 47 7 1 174 17 184 4.83e-62 190
MS.gene013115.t1 MTR_3g099580 65.730 178 49 5 1 174 17 186 3.54e-57 177
MS.gene013115.t1 MTR_3g099540 60.345 116 44 2 1 116 18 131 6.96e-39 129
MS.gene013115.t1 MTR_8g007020 46.951 164 74 5 12 169 26 182 5.06e-33 116
MS.gene013115.t1 MTR_8g007035 56.566 99 41 2 12 109 26 123 1.68e-30 110
MS.gene013115.t1 MTR_4g114870 46.610 118 58 2 8 121 27 143 1.23e-27 106
MS.gene013115.t1 MTR_4g114870 47.525 101 48 2 10 106 191 290 2.55e-25 100
MS.gene013115.t1 MTR_5g006040 39.496 119 69 2 5 121 21 138 5.11e-27 100
MS.gene013115.t1 MTR_1g090190 38.983 118 70 2 8 125 21 136 5.93e-27 100
MS.gene013115.t1 MTR_7g086140 43.000 100 57 0 10 109 26 125 2.63e-23 90.5
MS.gene013115.t1 MTR_7g086190 43.000 100 57 0 10 109 26 125 2.63e-23 90.5
MS.gene013115.t1 MTR_7g086160 41.748 103 60 0 7 109 24 126 3.03e-22 87.8
MS.gene013115.t1 MTR_1g105130 42.268 97 56 0 10 106 25 121 3.63e-22 87.4
MS.gene013115.t1 MTR_1g105120 42.268 97 56 0 10 106 25 121 3.63e-22 87.4
MS.gene013115.t1 MTR_8g099220 42.718 103 55 2 8 107 27 128 8.63e-22 85.9
MS.gene013115.t1 MTR_6g080660 46.154 104 54 2 7 110 25 126 8.64e-22 87.8
MS.gene013115.t1 MTR_1g104800 40.000 100 57 2 9 106 24 122 2.18e-21 86.3
MS.gene013115.t1 MTR_2g088990 39.259 135 72 5 7 139 22 148 3.67e-21 86.7
MS.gene013115.t1 MTR_6g083240 33.537 164 82 5 12 169 29 171 3.86e-21 85.5
MS.gene013115.t1 MTR_1g112700 38.318 107 64 2 8 113 31 136 5.42e-21 85.1
MS.gene013115.t1 MTR_7g086220 40.000 100 60 0 10 109 25 124 6.71e-21 84.3
MS.gene013115.t1 MTR_0334s0010 41.121 107 58 3 5 108 23 127 3.08e-19 80.9
MS.gene013115.t1 MTR_4g067200 38.793 116 64 4 1 110 19 133 2.54e-18 78.2
MS.gene013115.t1 MTR_4g066110 38.793 116 64 4 1 110 19 133 2.54e-18 78.2
MS.gene013115.t1 MTR_2g025580 37.037 108 66 2 1 107 18 124 2.16e-17 75.5
MS.gene013115.t1 MTR_6g023760 40.404 99 58 1 8 106 24 121 3.77e-17 75.1
MS.gene013115.t1 MTR_3g089035 43.158 95 50 3 10 104 33 123 4.02e-17 73.6
MS.gene013115.t1 MTR_2g083250 43.269 104 52 3 11 108 27 129 6.63e-17 75.9
MS.gene013115.t1 MTR_4g114830 39.560 91 49 2 11 95 30 120 9.26e-17 73.6
MS.gene013115.t1 MTR_7g086090 40.206 97 57 1 11 107 27 122 3.18e-16 73.9
MS.gene013115.t1 MTR_2g043730 40.625 96 53 3 10 105 32 123 8.17e-16 70.1
MS.gene013115.t1 MTR_1g090420 30.328 122 79 3 7 123 25 145 8.55e-16 72.4
MS.gene013115.t1 MTR_7g086280 38.776 98 59 1 10 107 26 122 8.72e-16 71.2
MS.gene013115.t1 MTR_7g086230 38.776 98 59 1 10 107 26 122 8.72e-16 71.2
MS.gene013115.t1 MTR_6g013420 36.000 125 69 4 8 123 36 158 9.95e-16 71.2
MS.gene013115.t1 MTR_8g463180 33.051 118 73 3 5 117 19 135 1.20e-15 73.2
MS.gene013115.t1 MTR_4g081100 38.889 90 51 1 11 96 33 122 1.35e-15 72.0
MS.gene013115.t1 MTR_2g101300 36.000 125 70 4 12 131 29 148 2.60e-15 70.5
MS.gene013115.t1 MTR_8g086360 29.878 164 110 4 12 173 26 186 6.11e-15 69.7
MS.gene013115.t1 MTR_7g086100 39.583 96 57 1 11 106 27 121 6.95e-15 70.1
MS.gene013115.t1 MTR_4g007250 36.538 104 64 2 4 107 23 124 7.39e-15 68.9
MS.gene013115.t1 MTR_8g088830 40.909 88 48 3 12 98 30 114 1.08e-14 67.4
MS.gene013115.t1 MTR_6g013200 35.294 119 71 4 11 127 36 150 1.11e-14 68.6
MS.gene013115.t1 MTR_2g043790 40.625 96 53 3 10 105 7 98 1.25e-14 66.6
MS.gene013115.t1 MTR_8g089110 37.500 96 56 3 10 105 28 119 2.10e-13 63.9
MS.gene013115.t1 MTR_3g089005 36.538 104 62 3 1 104 24 123 2.40e-13 63.9
MS.gene013115.t1 MTR_4g130780 34.783 92 59 1 24 115 48 138 2.43e-13 65.1
MS.gene013115.t1 MTR_4g130800 35.514 107 63 3 5 106 24 129 3.43e-13 65.9
MS.gene013115.t1 MTR_8g094990 33.929 112 70 2 12 120 27 137 4.59e-13 65.9
MS.gene013115.t1 MTR_4g077787 30.882 136 79 4 12 135 34 166 5.52e-13 64.3
MS.gene013115.t1 MTR_7g086200 34.821 112 57 2 10 121 4 99 6.87e-13 62.4
MS.gene013115.t1 MTR_4g130800 40.244 82 48 1 25 106 48 128 6.94e-13 65.1
MS.gene013115.t1 MTR_4g124280 32.967 91 57 1 12 98 27 117 3.92e-12 62.8
MS.gene013115.t1 MTR_6g022170 37.000 100 55 4 12 106 36 132 5.72e-12 61.2
MS.gene013115.t1 MTR_2g090575 35.955 89 55 1 12 98 27 115 7.05e-12 62.8
MS.gene013115.t1 MTR_2g090580 27.907 172 114 5 12 173 29 200 1.18e-11 60.8
MS.gene013115.t1 MTR_4g111640 30.147 136 80 4 12 135 34 166 1.59e-11 60.1
MS.gene013115.t1 MTR_6g013170 30.201 149 94 5 9 156 30 169 3.20e-11 59.3
MS.gene013115.t1 MTR_4g078410 29.134 127 87 2 12 136 27 152 3.40e-11 59.3
MS.gene013115.t1 MTR_1g014120 34.066 91 59 1 25 115 45 134 5.60e-11 58.9
MS.gene013115.t1 MTR_3g105930 31.132 106 69 2 25 127 47 151 5.72e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013115.t1 AT1G72230 54.386 114 44 3 18 131 31 136 2.86e-34 119
MS.gene013115.t1 AT1G22480 55.670 97 41 2 16 112 25 119 1.59e-27 102
MS.gene013115.t1 AT2G32300 40.909 132 71 3 5 131 21 150 1.80e-25 99.0
MS.gene013115.t1 AT2G32300 40.909 132 71 3 5 131 51 180 3.47e-25 98.6
MS.gene013115.t1 AT5G07475 38.636 132 69 3 8 129 28 157 3.89e-25 96.3
MS.gene013115.t1 AT2G31050 45.299 117 60 3 7 122 26 139 1.84e-24 94.7
MS.gene013115.t1 AT3G17675 40.566 106 62 1 1 106 23 127 1.25e-23 90.5
MS.gene013115.t1 AT2G26720 40.351 114 66 2 7 119 26 138 2.81e-23 91.7
MS.gene013115.t1 AT5G26330 41.284 109 61 2 5 110 19 127 7.40e-23 90.1
MS.gene013115.t1 AT2G44790 49.474 95 46 2 18 111 36 129 2.91e-21 86.7
MS.gene013115.t1 AT3G60270 47.959 98 49 2 12 109 28 123 4.37e-21 85.5
MS.gene013115.t1 AT3G27200 41.837 98 54 3 10 106 26 121 4.84e-19 80.1
MS.gene013115.t1 AT5G20230 41.463 123 66 3 12 131 27 146 2.67e-18 78.6
MS.gene013115.t1 AT3G60280 42.857 98 54 2 12 109 26 121 8.77e-16 72.4
MS.gene013115.t1 AT1G64640 35.772 123 74 3 8 127 31 151 1.80e-14 68.2
MS.gene013115.t1 AT4G28365 28.249 177 118 3 5 173 24 199 1.36e-13 66.2
MS.gene013115.t1 AT5G53870 30.909 110 74 1 12 119 32 141 1.38e-13 67.8
MS.gene013115.t1 AT2G25060 38.542 96 55 2 24 116 50 144 1.53e-13 65.9
MS.gene013115.t1 AT5G53870 30.909 110 74 1 12 119 32 141 1.80e-13 67.4
MS.gene013115.t1 AT5G15350 33.333 132 79 5 1 127 17 144 3.16e-13 64.7
MS.gene013115.t1 AT2G02850 38.384 99 57 3 6 104 32 126 3.89e-13 63.5
MS.gene013115.t1 AT3G01070 33.333 117 77 1 12 127 29 145 1.82e-12 62.4
MS.gene013115.t1 AT4G27520 34.021 97 61 2 12 106 33 128 2.20e-12 64.3
MS.gene013115.t1 AT4G31840 30.693 101 70 0 24 124 46 146 7.14e-12 61.2
MS.gene013115.t1 AT3G20570 30.769 156 97 3 12 157 32 186 1.26e-11 60.8
MS.gene013115.t1 AT4G30590 32.520 123 77 4 9 126 27 148 8.79e-11 58.5

Find 0 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATTCTGATTATACAACAT+GGG - 23849:28841-28860 MS.gene013115:CDS 25.0%
! TTGATTCTGATTATACAACA+TGG - 23849:28840-28859 MS.gene013115:CDS 25.0%
ACGAAAAATAAACCAACGAT+TGG + 23849:29348-29367 None:intergenic 30.0%
TCAATTAGTACAGATAGTAG+TGG - 23849:29069-29088 MS.gene013115:CDS 30.0%
ACAGTGTTCAATTATGAAGC+TGG - 23849:28988-29007 MS.gene013115:CDS 35.0%
AGTGACAAAACCTTTGTAGT+TGG - 23849:28866-28885 MS.gene013115:CDS 35.0%
CAGTGTTCAATTATGAAGCT+GGG - 23849:28989-29008 MS.gene013115:CDS 35.0%
GAAACTCTCTGTTAAGGTTA+AGG - 23849:29173-29192 MS.gene013115:intron 35.0%
! AGTTGAAGATGCATTTTGCA+TGG + 23849:29316-29335 None:intergenic 35.0%
!!! AATGAGTTCCTACTTTTTCG+AGG + 23849:29110-29129 None:intergenic 35.0%
!!! ATGAGTTCCTACTTTTTCGA+GGG + 23849:29109-29128 None:intergenic 35.0%
GGGTGCAATAGTAATTGTGT+CGG + 23849:29268-29287 None:intergenic 40.0%
GGTGCAATAGTAATTGTGTC+GGG + 23849:29267-29286 None:intergenic 40.0%
GTGCAATAGTAATTGTGTCG+GGG + 23849:29266-29285 None:intergenic 40.0%
GTTCCTGTACATTGCATAAG+TGG - 23849:29144-29163 MS.gene013115:CDS 40.0%
TGCAATAGTAATTGTGTCGG+GGG + 23849:29265-29284 None:intergenic 40.0%
TGGCATGAAACTCTCTGTTA+AGG - 23849:29167-29186 MS.gene013115:CDS 40.0%
TGTTCAATTATGAAGCTGGG+TGG - 23849:28992-29011 MS.gene013115:CDS 40.0%
! GAAGATGCATTTTGCATGGT+AGG + 23849:29312-29331 None:intergenic 40.0%
! TTGTTGGAGATGCTTTAGGT+TGG - 23849:28813-28832 MS.gene013115:CDS 40.0%
!! TTTGTAGTTGGTGACAGTCT+CGG - 23849:28878-28897 MS.gene013115:CDS 40.0%
!!! TGAGTTCCTACTTTTTCGAG+GGG + 23849:29108-29127 None:intergenic 40.0%
ACCATTCCCCTCGAAAAAGT+AGG - 23849:29099-29118 MS.gene013115:CDS 45.0%
CACGTTGTTGGAGATGCTTT+AGG - 23849:28809-28828 MS.gene013115:CDS 45.0%
CCACCACTTATGCAATGTAC+AGG + 23849:29150-29169 None:intergenic 45.0%
CCTGTACATTGCATAAGTGG+TGG - 23849:29147-29166 MS.gene013115:CDS 45.0%
GAGACTGTCACCAACTACAA+AGG + 23849:28879-28898 None:intergenic 45.0%
GGGAAAGGTTCACCTTCTAA+TGG - 23849:29237-29256 MS.gene013115:intron 45.0%
TTGCATGGTAGGAGTAGCAA+TGG + 23849:29301-29320 None:intergenic 45.0%
! ACTAGTCTCTCACCAATCGT+TGG - 23849:29333-29352 MS.gene013115:CDS 45.0%
! CTAAAGCATCTCCAACAACG+TGG + 23849:28811-28830 None:intergenic 45.0%
!!! TCCTACTTTTTCGAGGGGAA+TGG + 23849:29103-29122 None:intergenic 45.0%
!!! TTTTTCGAGGGGAATGGTTG+TGG + 23849:29097-29116 None:intergenic 45.0%
CATGGTAGGAGTAGCAATGG+TGG + 23849:29298-29317 None:intergenic 50.0%
CTTCAGCAACGCCATCATCA+GGG - 23849:29217-29236 MS.gene013115:intron 50.0%
GGTGAACCTTTCCCTGATGA+TGG + 23849:29231-29250 None:intergenic 50.0%
ACGTGGACAGTTCCCTGAGT+CGG + 23849:28794-28813 None:intergenic 55.0%
CAGGGAACTGTCCACGTTGT+TGG - 23849:28797-28816 MS.gene013115:CDS 55.0%
CCTTCAGCAACGCCATCATC+AGG - 23849:29216-29235 MS.gene013115:intron 55.0%
GCAACGCCATCATCAGGGAA+AGG - 23849:29222-29241 MS.gene013115:intron 55.0%
GGTAGGAGTAGCAATGGTGG+TGG + 23849:29295-29314 None:intergenic 55.0%
TGCTGAAGGAGCAGCAGAAG+CGG + 23849:29205-29224 None:intergenic 55.0%
!! AGGAGTAGCAATGGTGGTGG+TGG + 23849:29292-29311 None:intergenic 55.0%
!! AGTAGCAATGGTGGTGGTGG+AGG + 23849:29289-29308 None:intergenic 55.0%
!! CCTGATGATGGCGTTGCTGA+AGG + 23849:29219-29238 None:intergenic 55.0%
!! GTAGCAATGGTGGTGGTGGA+GGG + 23849:29288-29307 None:intergenic 55.0%
TGAAGCTGGGTGGCACACAG+TGG - 23849:29002-29021 MS.gene013115:CDS 60.0%
!! GTGTCGGGGGTGCCATTAGA+AGG + 23849:29252-29271 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
23849 gene 28782 29398 28782 ID=MS.gene013115
23849 mRNA 28782 29398 28782 ID=MS.gene013115.t1;Parent=MS.gene013115
23849 exon 29281 29398 29281 ID=MS.gene013115.t1.exon1;Parent=MS.gene013115.t1
23849 CDS 29281 29398 29281 ID=cds.MS.gene013115.t1;Parent=MS.gene013115.t1
23849 exon 28782 29188 28782 ID=MS.gene013115.t1.exon2;Parent=MS.gene013115.t1
23849 CDS 28782 29188 28782 ID=cds.MS.gene013115.t1;Parent=MS.gene013115.t1
Gene Sequence

>MS.gene013115

ATGGCATTGCCGACTCAGGGAACTGTCCACGTTGTTGGAGATGCTTTAGGTTGGACACTTGATTCTGATTATACAACATGGGCTAGTGACAAAACCTTTGTAGTTGGTGACAGTCTCGGTAAGTATCATAATTAATTGAAGTTTATCAATTCTATTGTTTGTTTAGTTTTACAATTTACAAAACTAATGTATGTAACTTTACTATGACAGTGTTCAATTATGAAGCTGGGTGGCACACAGTGGATGAAGTTAGAGAAAGTGACTATAAAACATGCACAACTAGAGATTCAATTAGTACAGATAGTAGTGGTGCCACAACCATTCCCCTCGAAAAAGTAGGAACTCATTACTTCATATGTGCTGTTCCTGTACATTGCATAAGTGGTGGCATGAAACTCTCTGTTAAGGTTAAGGCTTCTTCCGCTTCTGCTGCTCCTTCAGCAACGCCATCATCAGGGAAAGGTTCACCTTCTAATGGCACCCCCGACACAATTACTATTGCACCCTCCACCACCACCATTGCTACTCCTACCATGCAAAATGCATCTTCAACTAGTCTCTCACCAATCGTTGGTTTATTTTTCGTTGTTATGTTGATCAGTTATATATTGATGTGA

Protein sequence

>MS.gene013115.t1

MALPTQGTVHVVGDALGWTLDSDYTTWASDKTFVVGDSLVFNYEAGWHTVDEVRESDYKTCTTRDSISTDSSGATTIPLEKVGTHYFICAVPVHCISGGMKLSVKVKASSASAAPSATPSSGKGSPSNGTPDTITIAPSTTTIATPTMQNASSTSLSPIVGLFFVVMLISYILM