Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47150.t1 | XP_003618846.1 | 90.7 | 183 | 12 | 2 | 1 | 182 | 1 | 179 | 4.10E-57 | 231.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47150.t1 | O82081 | 40.5 | 116 | 67 | 2 | 6 | 120 | 7 | 121 | 3.4e-20 | 99.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47150.t1 | G7KNP1 | 90.7 | 183 | 12 | 2 | 1 | 182 | 1 | 179 | 3.0e-57 | 231.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene47150 | MS.gene054805 | PPI |
| MS.gene47150 | MS.gene38580 | PPI |
| MS.gene47150 | MS.gene054800 | PPI |
| MS.gene020466 | MS.gene47150 | PPI |
| MS.gene47150 | MS.gene05286 | PPI |
| MS.gene47150 | MS.gene054799 | PPI |
| MS.gene47150 | MS.gene054798 | PPI |
| MS.gene47150 | MS.gene013126 | PPI |
| MS.gene47150 | MS.gene38582 | PPI |
| MS.gene58908 | MS.gene47150 | PPI |
| MS.gene47150 | MS.gene58863 | PPI |
| MS.gene47150 | MS.gene013115 | PPI |
| MS.gene47150 | MS.gene38581 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47150.t1 | MTR_6g023760 | 85.246 | 183 | 22 | 2 | 1 | 182 | 1 | 179 | 3.37e-86 | 251 |
| MS.gene47150.t1 | MTR_2g088990 | 40.876 | 137 | 79 | 2 | 1 | 136 | 1 | 136 | 1.45e-30 | 111 |
| MS.gene47150.t1 | MTR_6g080660 | 39.474 | 114 | 68 | 1 | 7 | 120 | 10 | 122 | 7.50e-30 | 109 |
| MS.gene47150.t1 | MTR_8g007020 | 38.764 | 178 | 91 | 5 | 8 | 170 | 9 | 183 | 3.98e-27 | 101 |
| MS.gene47150.t1 | MTR_5g006040 | 34.637 | 179 | 110 | 5 | 4 | 177 | 6 | 182 | 4.22e-27 | 101 |
| MS.gene47150.t1 | MTR_2g025580 | 29.480 | 173 | 110 | 3 | 7 | 176 | 7 | 170 | 4.67e-26 | 98.2 |
| MS.gene47150.t1 | MTR_8g007035 | 46.087 | 115 | 57 | 3 | 8 | 120 | 9 | 120 | 2.57e-24 | 94.7 |
| MS.gene47150.t1 | MTR_3g099570 | 38.971 | 136 | 76 | 3 | 6 | 138 | 7 | 138 | 3.61e-24 | 94.0 |
| MS.gene47150.t1 | MTR_3g099580 | 43.220 | 118 | 60 | 3 | 6 | 120 | 7 | 120 | 6.34e-23 | 90.5 |
| MS.gene47150.t1 | MTR_3g099540 | 39.837 | 123 | 70 | 2 | 8 | 130 | 13 | 131 | 1.17e-21 | 85.9 |
| MS.gene47150.t1 | MTR_7g086140 | 32.773 | 119 | 77 | 3 | 4 | 120 | 5 | 122 | 8.80e-21 | 84.3 |
| MS.gene47150.t1 | MTR_7g086190 | 32.773 | 119 | 77 | 3 | 4 | 120 | 5 | 122 | 8.80e-21 | 84.3 |
| MS.gene47150.t1 | MTR_1g090190 | 29.193 | 161 | 112 | 2 | 22 | 181 | 20 | 179 | 1.82e-20 | 84.0 |
| MS.gene47150.t1 | MTR_3g099980 | 40.496 | 121 | 65 | 3 | 4 | 120 | 5 | 122 | 2.56e-20 | 84.7 |
| MS.gene47150.t1 | MTR_7g086220 | 31.967 | 122 | 80 | 3 | 1 | 120 | 1 | 121 | 4.20e-20 | 82.4 |
| MS.gene47150.t1 | MTR_3g099680 | 40.496 | 121 | 65 | 3 | 4 | 120 | 5 | 122 | 4.27e-20 | 84.0 |
| MS.gene47150.t1 | MTR_1g104800 | 30.323 | 155 | 104 | 3 | 22 | 173 | 22 | 175 | 6.26e-20 | 82.8 |
| MS.gene47150.t1 | MTR_8g086360 | 30.000 | 180 | 114 | 4 | 8 | 175 | 5 | 184 | 3.55e-19 | 80.9 |
| MS.gene47150.t1 | MTR_7g086160 | 35.922 | 103 | 63 | 3 | 20 | 120 | 22 | 123 | 7.95e-19 | 79.3 |
| MS.gene47150.t1 | MTR_4g007250 | 35.354 | 99 | 63 | 1 | 22 | 120 | 26 | 123 | 1.11e-18 | 79.3 |
| MS.gene47150.t1 | MTR_2g101300 | 34.641 | 153 | 89 | 6 | 6 | 153 | 10 | 156 | 2.40e-18 | 78.6 |
| MS.gene47150.t1 | MTR_1g105130 | 29.143 | 175 | 96 | 5 | 7 | 179 | 7 | 155 | 2.52e-18 | 77.8 |
| MS.gene47150.t1 | MTR_1g105120 | 29.143 | 175 | 96 | 5 | 7 | 179 | 7 | 155 | 2.52e-18 | 77.8 |
| MS.gene47150.t1 | MTR_4g067200 | 35.294 | 136 | 74 | 5 | 6 | 134 | 9 | 137 | 2.96e-18 | 78.2 |
| MS.gene47150.t1 | MTR_4g066110 | 35.294 | 136 | 74 | 5 | 6 | 134 | 9 | 137 | 2.96e-18 | 78.2 |
| MS.gene47150.t1 | MTR_4g114870 | 34.074 | 135 | 85 | 1 | 6 | 136 | 10 | 144 | 3.07e-18 | 81.3 |
| MS.gene47150.t1 | MTR_4g114870 | 37.624 | 101 | 57 | 3 | 25 | 120 | 191 | 290 | 3.30e-16 | 75.5 |
| MS.gene47150.t1 | MTR_0334s0010 | 35.593 | 118 | 72 | 3 | 6 | 120 | 9 | 125 | 3.43e-18 | 78.6 |
| MS.gene47150.t1 | MTR_8g088830 | 44.231 | 104 | 48 | 6 | 20 | 119 | 23 | 120 | 4.11e-18 | 76.3 |
| MS.gene47150.t1 | MTR_4g077787 | 31.690 | 142 | 87 | 3 | 23 | 158 | 30 | 167 | 8.00e-18 | 77.0 |
| MS.gene47150.t1 | MTR_2g043730 | 35.345 | 116 | 70 | 3 | 6 | 119 | 11 | 123 | 1.62e-16 | 72.4 |
| MS.gene47150.t1 | MTR_3g089005 | 37.069 | 116 | 67 | 5 | 6 | 119 | 13 | 124 | 1.64e-16 | 72.4 |
| MS.gene47150.t1 | MTR_6g013170 | 36.220 | 127 | 78 | 3 | 25 | 150 | 31 | 155 | 1.75e-16 | 73.6 |
| MS.gene47150.t1 | MTR_4g111640 | 31.343 | 134 | 84 | 3 | 23 | 150 | 30 | 161 | 1.88e-16 | 73.6 |
| MS.gene47150.t1 | MTR_7g086100 | 29.060 | 117 | 83 | 0 | 4 | 120 | 5 | 121 | 2.19e-16 | 74.3 |
| MS.gene47150.t1 | MTR_7g086090 | 31.405 | 121 | 82 | 1 | 1 | 120 | 1 | 121 | 3.68e-16 | 73.9 |
| MS.gene47150.t1 | MTR_3g089035 | 40.816 | 98 | 53 | 4 | 22 | 118 | 30 | 123 | 4.91e-16 | 71.2 |
| MS.gene47150.t1 | MTR_5g088000 | 31.387 | 137 | 89 | 5 | 6 | 139 | 1 | 135 | 1.15e-15 | 70.5 |
| MS.gene47150.t1 | MTR_8g463180 | 32.414 | 145 | 85 | 5 | 1 | 142 | 1 | 135 | 2.59e-15 | 72.4 |
| MS.gene47150.t1 | MTR_6g013420 | 30.147 | 136 | 90 | 4 | 6 | 137 | 17 | 151 | 3.00e-15 | 70.5 |
| MS.gene47150.t1 | MTR_3g105930 | 33.577 | 137 | 83 | 4 | 22 | 150 | 24 | 160 | 7.12e-15 | 69.3 |
| MS.gene47150.t1 | MTR_2g090580 | 25.131 | 191 | 125 | 3 | 6 | 178 | 8 | 198 | 8.80e-15 | 69.3 |
| MS.gene47150.t1 | MTR_1g112700 | 32.456 | 114 | 74 | 3 | 9 | 120 | 17 | 129 | 1.06e-14 | 68.9 |
| MS.gene47150.t1 | MTR_8g094990 | 31.356 | 118 | 76 | 2 | 8 | 120 | 6 | 123 | 1.49e-14 | 70.1 |
| MS.gene47150.t1 | MTR_4g078410 | 27.848 | 158 | 105 | 3 | 22 | 170 | 22 | 179 | 1.77e-14 | 68.6 |
| MS.gene47150.t1 | MTR_8g095013 | 30.508 | 118 | 77 | 2 | 8 | 120 | 6 | 123 | 1.91e-14 | 70.1 |
| MS.gene47150.t1 | MTR_2g043790 | 37.374 | 99 | 59 | 2 | 21 | 119 | 3 | 98 | 2.65e-14 | 65.9 |
| MS.gene47150.t1 | MTR_2g090575 | 33.582 | 134 | 79 | 5 | 6 | 131 | 7 | 138 | 3.14e-14 | 69.7 |
| MS.gene47150.t1 | MTR_7g086230 | 28.926 | 121 | 85 | 1 | 1 | 120 | 1 | 121 | 4.23e-14 | 67.0 |
| MS.gene47150.t1 | MTR_7g086280 | 28.926 | 121 | 85 | 1 | 1 | 120 | 1 | 121 | 4.50e-14 | 67.0 |
| MS.gene47150.t1 | MTR_6g022170 | 35.345 | 116 | 66 | 5 | 20 | 128 | 29 | 142 | 8.58e-14 | 66.2 |
| MS.gene47150.t1 | MTR_4g112310 | 31.250 | 112 | 75 | 2 | 39 | 150 | 2 | 111 | 1.03e-13 | 65.1 |
| MS.gene47150.t1 | MTR_1g014120 | 32.624 | 141 | 81 | 6 | 6 | 137 | 11 | 146 | 1.17e-13 | 66.2 |
| MS.gene47150.t1 | MTR_2g083250 | 35.000 | 100 | 59 | 3 | 27 | 120 | 28 | 127 | 1.39e-13 | 67.4 |
| MS.gene47150.t1 | MTR_8g099220 | 37.864 | 103 | 59 | 4 | 22 | 120 | 26 | 127 | 1.37e-12 | 62.0 |
| MS.gene47150.t1 | MTR_2g079030 | 28.662 | 157 | 104 | 3 | 4 | 153 | 5 | 160 | 1.57e-12 | 65.1 |
| MS.gene47150.t1 | MTR_4g081100 | 34.694 | 98 | 60 | 1 | 27 | 120 | 34 | 131 | 1.85e-12 | 63.5 |
| MS.gene47150.t1 | MTR_1g077790 | 33.036 | 112 | 68 | 4 | 16 | 121 | 20 | 130 | 6.77e-12 | 61.6 |
| MS.gene47150.t1 | MTR_7g086200 | 35.484 | 93 | 57 | 3 | 46 | 136 | 10 | 101 | 7.29e-12 | 60.1 |
| MS.gene47150.t1 | MTR_4g124280 | 27.778 | 162 | 110 | 4 | 1 | 156 | 2 | 162 | 2.84e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47150.t1 | AT3G27200 | 62.609 | 115 | 43 | 0 | 6 | 120 | 7 | 121 | 2.95e-51 | 162 |
| MS.gene47150.t1 | AT2G32300 | 37.500 | 144 | 86 | 3 | 4 | 144 | 5 | 147 | 3.08e-30 | 111 |
| MS.gene47150.t1 | AT2G32300 | 37.500 | 144 | 86 | 3 | 4 | 144 | 35 | 177 | 1.11e-29 | 110 |
| MS.gene47150.t1 | AT5G07475 | 35.714 | 154 | 94 | 3 | 11 | 163 | 16 | 165 | 3.88e-28 | 104 |
| MS.gene47150.t1 | AT2G31050 | 40.800 | 125 | 71 | 3 | 9 | 131 | 13 | 136 | 4.40e-24 | 94.0 |
| MS.gene47150.t1 | AT2G26720 | 37.195 | 164 | 96 | 5 | 4 | 163 | 6 | 166 | 2.33e-23 | 92.4 |
| MS.gene47150.t1 | AT4G31840 | 34.637 | 179 | 100 | 8 | 7 | 179 | 10 | 177 | 2.90e-21 | 86.3 |
| MS.gene47150.t1 | AT1G72230 | 30.899 | 178 | 120 | 3 | 5 | 181 | 3 | 178 | 2.60e-20 | 83.6 |
| MS.gene47150.t1 | AT3G18590 | 38.378 | 185 | 105 | 5 | 4 | 181 | 5 | 187 | 1.41e-19 | 82.0 |
| MS.gene47150.t1 | AT1G48940 | 31.383 | 188 | 110 | 4 | 1 | 181 | 1 | 176 | 4.44e-19 | 80.5 |
| MS.gene47150.t1 | AT5G26330 | 31.667 | 180 | 111 | 5 | 7 | 175 | 4 | 182 | 6.38e-19 | 80.1 |
| MS.gene47150.t1 | AT3G60270 | 40.594 | 101 | 59 | 1 | 20 | 120 | 21 | 120 | 2.19e-18 | 79.0 |
| MS.gene47150.t1 | AT5G53870 | 32.432 | 148 | 94 | 3 | 3 | 145 | 9 | 155 | 2.96e-18 | 81.3 |
| MS.gene47150.t1 | AT2G27035 | 31.159 | 138 | 88 | 6 | 6 | 140 | 9 | 142 | 3.80e-18 | 77.8 |
| MS.gene47150.t1 | AT5G53870 | 32.432 | 148 | 94 | 3 | 3 | 145 | 9 | 155 | 4.51e-18 | 80.9 |
| MS.gene47150.t1 | AT3G17675 | 35.593 | 118 | 71 | 4 | 6 | 120 | 12 | 127 | 6.41e-18 | 76.3 |
| MS.gene47150.t1 | AT3G60280 | 38.614 | 101 | 61 | 1 | 20 | 120 | 19 | 118 | 1.45e-17 | 77.4 |
| MS.gene47150.t1 | AT1G45063 | 36.441 | 118 | 70 | 4 | 19 | 131 | 141 | 258 | 1.46e-17 | 78.2 |
| MS.gene47150.t1 | AT1G45063 | 31.304 | 115 | 77 | 2 | 8 | 120 | 12 | 126 | 4.57e-12 | 63.2 |
| MS.gene47150.t1 | AT1G22480 | 41.489 | 94 | 54 | 1 | 33 | 126 | 27 | 119 | 1.51e-17 | 76.3 |
| MS.gene47150.t1 | AT1G45063 | 36.441 | 118 | 70 | 4 | 19 | 131 | 141 | 258 | 2.67e-17 | 78.6 |
| MS.gene47150.t1 | AT1G45063 | 31.304 | 115 | 77 | 2 | 8 | 120 | 12 | 126 | 5.69e-12 | 63.5 |
| MS.gene47150.t1 | AT2G25060 | 32.778 | 180 | 99 | 7 | 6 | 179 | 19 | 182 | 1.85e-16 | 73.6 |
| MS.gene47150.t1 | AT2G44790 | 40.000 | 95 | 54 | 2 | 33 | 125 | 36 | 129 | 2.58e-16 | 73.6 |
| MS.gene47150.t1 | AT5G14345 | 34.815 | 135 | 74 | 6 | 7 | 133 | 1 | 129 | 1.63e-15 | 70.1 |
| MS.gene47150.t1 | AT4G12880 | 34.188 | 117 | 72 | 5 | 7 | 120 | 11 | 125 | 2.86e-15 | 69.3 |
| MS.gene47150.t1 | AT4G28365 | 31.765 | 170 | 98 | 7 | 27 | 179 | 31 | 199 | 3.08e-14 | 68.2 |
| MS.gene47150.t1 | AT5G57920 | 30.216 | 139 | 85 | 5 | 18 | 149 | 53 | 186 | 5.71e-14 | 67.8 |
| MS.gene47150.t1 | AT5G20230 | 26.613 | 124 | 88 | 1 | 24 | 144 | 24 | 147 | 6.04e-14 | 67.4 |
| MS.gene47150.t1 | AT3G53330 | 32.500 | 120 | 77 | 3 | 25 | 141 | 188 | 306 | 9.98e-14 | 68.2 |
| MS.gene47150.t1 | AT3G53330 | 36.842 | 95 | 57 | 2 | 1 | 94 | 1 | 93 | 9.06e-11 | 59.7 |
| MS.gene47150.t1 | AT1G08500 | 28.488 | 172 | 102 | 3 | 3 | 153 | 8 | 179 | 1.50e-13 | 66.6 |
| MS.gene47150.t1 | AT5G57920 | 29.204 | 113 | 78 | 1 | 39 | 149 | 41 | 153 | 1.67e-13 | 65.9 |
| MS.gene47150.t1 | AT5G57920 | 29.204 | 113 | 78 | 1 | 39 | 149 | 42 | 154 | 1.74e-13 | 65.9 |
| MS.gene47150.t1 | AT2G23990 | 34.146 | 123 | 68 | 6 | 26 | 141 | 28 | 144 | 1.86e-13 | 66.2 |
| MS.gene47150.t1 | AT1G17800 | 32.456 | 114 | 73 | 3 | 7 | 118 | 23 | 134 | 2.90e-13 | 64.3 |
| MS.gene47150.t1 | AT4G32490 | 39.175 | 97 | 55 | 3 | 27 | 120 | 33 | 128 | 3.63e-13 | 65.5 |
| MS.gene47150.t1 | AT4G27520 | 32.203 | 118 | 74 | 4 | 8 | 120 | 12 | 128 | 6.65e-13 | 66.2 |
| MS.gene47150.t1 | AT4G30590 | 35.000 | 100 | 58 | 4 | 27 | 120 | 30 | 128 | 8.45e-13 | 63.9 |
| MS.gene47150.t1 | AT5G25090 | 31.667 | 120 | 72 | 3 | 6 | 120 | 12 | 126 | 9.43e-13 | 63.9 |
| MS.gene47150.t1 | AT2G02850 | 32.773 | 119 | 73 | 5 | 4 | 119 | 13 | 127 | 1.90e-12 | 61.6 |
| MS.gene47150.t1 | AT1G64640 | 31.746 | 126 | 80 | 4 | 19 | 139 | 27 | 151 | 3.81e-12 | 62.4 |
| MS.gene47150.t1 | AT3G20570 | 30.172 | 116 | 78 | 2 | 8 | 120 | 13 | 128 | 1.82e-11 | 60.8 |
Find 40 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAAAAGATGTTGTGAAAT+TGG | 0.298026 | 6.3:+16923421 | MS.gene47150:CDS |
| CATCCTCAGACTTCGATTCA+TGG | 0.350452 | 6.3:+16922728 | MS.gene47150:CDS |
| CAATACCTCCTTTGTGATCA+AGG | 0.383649 | 6.3:-16922665 | None:intergenic |
| GACTTCGATTCATGGAGTTC+AGG | 0.393109 | 6.3:+16922736 | MS.gene47150:CDS |
| GTAAAGATCACAGTAGGAAA+TGG | 0.422825 | 6.3:+16923506 | MS.gene47150:CDS |
| CTTGATCACAAAGGAGGTAT+TGG | 0.426661 | 6.3:+16922666 | MS.gene47150:CDS |
| CTGAAGATGTATCAGATGTA+GGG | 0.476612 | 6.3:-16923616 | None:intergenic |
| GCTGAAGATGTATCAGATGT+AGG | 0.484922 | 6.3:-16923617 | None:intergenic |
| CCAGTGCTTAAGGAATTTCG+TGG | 0.487279 | 6.3:-16923401 | None:intergenic |
| TACTTCACATGTGGTACCTT+AGG | 0.496106 | 6.3:+16923461 | MS.gene47150:CDS |
| TCAGGTCAAACATTCAAAGT+TGG | 0.497486 | 6.3:+16922754 | MS.gene47150:CDS |
| CAGTGCTTAAGGAATTTCGT+GGG | 0.508360 | 6.3:-16923400 | None:intergenic |
| ACCTTAGGTCACTGTGGTCA+AGG | 0.513068 | 6.3:+16923476 | MS.gene47150:CDS |
| CATAATGTTGGTGGAAGTCA+AGG | 0.516548 | 6.3:+16922697 | MS.gene47150:CDS |
| TTCAAAGTTGGAGATCAACT+TGG | 0.523708 | 6.3:+16922766 | MS.gene47150:CDS |
| CCACGAAATTCCTTAAGCAC+TGG | 0.526551 | 6.3:+16923401 | MS.gene47150:CDS |
| ACCTTGACCACAGTGACCTA+AGG | 0.528898 | 6.3:-16923477 | None:intergenic |
| TGTTGGTGGAAGTCAAGGTT+GGG | 0.547956 | 6.3:+16922702 | MS.gene47150:CDS |
| AATTATGTTCTTGCACCCCA+TGG | 0.558852 | 6.3:-16922623 | None:intergenic |
| TCACTGTGGTCAAGGTATGA+AGG | 0.561109 | 6.3:+16923484 | MS.gene47150:CDS |
| TAGTTCAACCACACTATGCA+TGG | 0.572033 | 6.3:-16923339 | None:intergenic |
| ATGTTGGTGGAAGTCAAGGT+TGG | 0.572733 | 6.3:+16922701 | MS.gene47150:CDS |
| CATGTGAAGTACCTTGTACC+AGG | 0.577155 | 6.3:-16923449 | None:intergenic |
| CATGAATCGAAGTCTGAGGA+TGG | 0.577228 | 6.3:-16922727 | None:intergenic |
| GCAACACAACATAATGTTGG+TGG | 0.579218 | 6.3:+16922688 | MS.gene47150:CDS |
| ATGAAGGTAAAGATCACAGT+AGG | 0.591873 | 6.3:+16923500 | MS.gene47150:CDS |
| TTGGCAACACAACATAATGT+TGG | 0.615720 | 6.3:+16922685 | MS.gene47150:CDS |
| AATTGGACAAGCCTGGTACA+AGG | 0.619587 | 6.3:+16923438 | MS.gene47150:CDS |
| TGAAGATGTATCAGATGTAG+GGG | 0.632402 | 6.3:-16923615 | None:intergenic |
| ACTCCATGAATCGAAGTCTG+AGG | 0.645169 | 6.3:-16922731 | None:intergenic |
| AGCTACCTTGATCACAAAGG+AGG | 0.662032 | 6.3:+16922660 | MS.gene47150:CDS |
| GTACACATCCATGCATAGTG+TGG | 0.662604 | 6.3:+16923331 | MS.gene47150:CDS |
| GTTAGCTACCTTGATCACAA+AGG | 0.669540 | 6.3:+16922657 | MS.gene47150:CDS |
| AGTGCTTAAGGAATTTCGTG+GGG | 0.682504 | 6.3:-16923399 | None:intergenic |
| GGTACAAGGTACTTCACATG+TGG | 0.682637 | 6.3:+16923452 | MS.gene47150:CDS |
| GTTGTGAAATTGGACAAGCC+TGG | 0.688360 | 6.3:+16923431 | MS.gene47150:CDS |
| AATAGAAGCAAAGTACTGTG+AGG | 0.719092 | 6.3:-16923639 | None:intergenic |
| GATGACGATGATGACGACGA+TGG | 0.722472 | 6.3:-16923551 | None:intergenic |
| TGTGGTACCTTAGGTCACTG+TGG | 0.739064 | 6.3:+16923470 | MS.gene47150:CDS |
| GACGACGATGAAGATGACGA+TGG | 0.793192 | 6.3:-16923578 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGCAAATAAATAATGT+TGG | + | chr6.3:16922956-16922975 | MS.gene47150:intron | 15.0% |
| !! | GTTTCTTATAAAAAATTTGT+TGG | + | chr6.3:16922792-16922811 | MS.gene47150:intron | 15.0% |
| !! | TTTACATAATTATGTTTACT+AGG | + | chr6.3:16922903-16922922 | MS.gene47150:intron | 15.0% |
| ! | ATATGATTGAATAACTCAAC+TGG | + | chr6.3:16923065-16923084 | MS.gene47150:intron | 25.0% |
| ! | GTTAGTATATTCTCCTTAAT+CGG | - | chr6.3:16923135-16923154 | None:intergenic | 25.0% |
| ! | TACGTCTATACAAAAAATTG+TGG | + | chr6.3:16923168-16923187 | MS.gene47150:intron | 25.0% |
| !!! | GCTAATATCACATTTTTTGT+AGG | - | chr6.3:16923378-16923397 | None:intergenic | 25.0% |
| ATTTCTCCACTTTCTACTAT+AGG | - | chr6.3:16922856-16922875 | None:intergenic | 30.0% | |
| CCATTGTTCAAAACTTTGAT+TGG | - | chr6.3:16922986-16923005 | None:intergenic | 30.0% | |
| GAAATTCCTATAGTAGAAAG+TGG | + | chr6.3:16922847-16922866 | MS.gene47150:intron | 30.0% | |
| GCAATCACAATTTATAACCT+TGG | + | chr6.3:16923218-16923237 | MS.gene47150:intron | 30.0% | |
| TATAACCTTGGATGTGATTA+TGG | + | chr6.3:16923230-16923249 | MS.gene47150:intron | 30.0% | |
| TGAACAATGGTATTGTAGAT+GGG | + | chr6.3:16922996-16923015 | MS.gene47150:intron | 30.0% | |
| TGGAAAAGATGTTGTGAAAT+TGG | + | chr6.3:16923421-16923440 | MS.gene47150:CDS | 30.0% | |
| TTGAACAATGGTATTGTAGA+TGG | + | chr6.3:16922995-16923014 | MS.gene47150:intron | 30.0% | |
| TTGTTCAAAACTTTGATTGG+TGG | - | chr6.3:16922983-16923002 | None:intergenic | 30.0% | |
| ! | ACTCAACTGGTTTAAACTTA+TGG | + | chr6.3:16923078-16923097 | MS.gene47150:intron | 30.0% |
| ! | GTGCAAGAACATAATTTTCT+TGG | + | chr6.3:16922630-16922649 | MS.gene47150:CDS | 30.0% |
| !!! | CCAATCAAAGTTTTGAACAA+TGG | + | chr6.3:16922983-16923002 | MS.gene47150:intron | 30.0% |
| ATGAAGGTAAAGATCACAGT+AGG | + | chr6.3:16923500-16923519 | MS.gene47150:CDS | 35.0% | |
| CTGAAGATGTATCAGATGTA+GGG | - | chr6.3:16923619-16923638 | None:intergenic | 35.0% | |
| GTAAAGATCACAGTAGGAAA+TGG | + | chr6.3:16923506-16923525 | MS.gene47150:CDS | 35.0% | |
| TCAGGTCAAACATTCAAAGT+TGG | + | chr6.3:16922754-16922773 | MS.gene47150:CDS | 35.0% | |
| TCTTTCCATAATCACATCCA+AGG | - | chr6.3:16923238-16923257 | None:intergenic | 35.0% | |
| TGAAGATGTATCAGATGTAG+GGG | - | chr6.3:16923618-16923637 | None:intergenic | 35.0% | |
| TTCAAAGTTGGAGATCAACT+TGG | + | chr6.3:16922766-16922785 | MS.gene47150:CDS | 35.0% | |
| TTGGCAACACAACATAATGT+TGG | + | chr6.3:16922685-16922704 | MS.gene47150:CDS | 35.0% | |
| ! | AACATCTTTTCCAGTGCTTA+AGG | - | chr6.3:16923414-16923433 | None:intergenic | 35.0% |
| !! | AATAGAAGCAAAGTACTGTG+AGG | - | chr6.3:16923642-16923661 | None:intergenic | 35.0% |
| !! | TTGAGTTGAAACTCCGATTA+AGG | + | chr6.3:16923119-16923138 | MS.gene47150:intron | 35.0% |
| AGTGCTTAAGGAATTTCGTG+GGG | - | chr6.3:16923402-16923421 | None:intergenic | 40.0% | |
| ATATTGCTACAACTGAGTCG+TGG | - | chr6.3:16923049-16923068 | None:intergenic | 40.0% | |
| CAATACCTCCTTTGTGATCA+AGG | - | chr6.3:16922668-16922687 | None:intergenic | 40.0% | |
| CAGTGCTTAAGGAATTTCGT+GGG | - | chr6.3:16923403-16923422 | None:intergenic | 40.0% | |
| GCAACACAACATAATGTTGG+TGG | + | chr6.3:16922688-16922707 | MS.gene47150:CDS | 40.0% | |
| GCTGAAGATGTATCAGATGT+AGG | - | chr6.3:16923620-16923639 | None:intergenic | 40.0% | |
| GTTAGCTACCTTGATCACAA+AGG | + | chr6.3:16922657-16922676 | MS.gene47150:CDS | 40.0% | |
| TACTTCACATGTGGTACCTT+AGG | + | chr6.3:16923461-16923480 | MS.gene47150:CDS | 40.0% | |
| TAGTTCAACCACACTATGCA+TGG | - | chr6.3:16923342-16923361 | None:intergenic | 40.0% | |
| ! | CATAATGTTGGTGGAAGTCA+AGG | + | chr6.3:16922697-16922716 | MS.gene47150:CDS | 40.0% |
| ! | CTTGATCACAAAGGAGGTAT+TGG | + | chr6.3:16922666-16922685 | MS.gene47150:CDS | 40.0% |
| AATTGGACAAGCCTGGTACA+AGG | + | chr6.3:16923438-16923457 | MS.gene47150:CDS | 45.0% | |
| ACTCCATGAATCGAAGTCTG+AGG | - | chr6.3:16922734-16922753 | None:intergenic | 45.0% | |
| AGCTACCTTGATCACAAAGG+AGG | + | chr6.3:16922660-16922679 | MS.gene47150:CDS | 45.0% | |
| CATCCTCAGACTTCGATTCA+TGG | + | chr6.3:16922728-16922747 | MS.gene47150:CDS | 45.0% | |
| CATGAATCGAAGTCTGAGGA+TGG | - | chr6.3:16922730-16922749 | None:intergenic | 45.0% | |
| CCACGAAATTCCTTAAGCAC+TGG | + | chr6.3:16923401-16923420 | MS.gene47150:CDS | 45.0% | |
| CCAGTGCTTAAGGAATTTCG+TGG | - | chr6.3:16923404-16923423 | None:intergenic | 45.0% | |
| GACTTCGATTCATGGAGTTC+AGG | + | chr6.3:16922736-16922755 | MS.gene47150:CDS | 45.0% | |
| GTACACATCCATGCATAGTG+TGG | + | chr6.3:16923331-16923350 | MS.gene47150:CDS | 45.0% | |
| GTTGTGAAATTGGACAAGCC+TGG | + | chr6.3:16923431-16923450 | MS.gene47150:CDS | 45.0% | |
| TCACTGTGGTCAAGGTATGA+AGG | + | chr6.3:16923484-16923503 | MS.gene47150:CDS | 45.0% | |
| ! | ATGTTGGTGGAAGTCAAGGT+TGG | + | chr6.3:16922701-16922720 | MS.gene47150:CDS | 45.0% |
| ! | CATGTGAAGTACCTTGTACC+AGG | - | chr6.3:16923452-16923471 | None:intergenic | 45.0% |
| ! | GGTACAAGGTACTTCACATG+TGG | + | chr6.3:16923452-16923471 | MS.gene47150:CDS | 45.0% |
| ! | TGTTGGTGGAAGTCAAGGTT+GGG | + | chr6.3:16922702-16922721 | MS.gene47150:CDS | 45.0% |
| ACCTTAGGTCACTGTGGTCA+AGG | + | chr6.3:16923476-16923495 | MS.gene47150:CDS | 50.0% | |
| ACCTTGACCACAGTGACCTA+AGG | - | chr6.3:16923480-16923499 | None:intergenic | 50.0% | |
| GACGACGATGAAGATGACGA+TGG | - | chr6.3:16923581-16923600 | None:intergenic | 50.0% | |
| TGTGGTACCTTAGGTCACTG+TGG | + | chr6.3:16923470-16923489 | MS.gene47150:CDS | 50.0% | |
| ! | GATGACGATGATGACGACGA+TGG | - | chr6.3:16923554-16923573 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 16922625 | 16923709 | 16922625 | ID=MS.gene47150 |
| chr6.3 | mRNA | 16922625 | 16923709 | 16922625 | ID=MS.gene47150.t1;Parent=MS.gene47150 |
| chr6.3 | exon | 16922625 | 16922787 | 16922625 | ID=MS.gene47150.t1.exon1;Parent=MS.gene47150.t1 |
| chr6.3 | CDS | 16922625 | 16922787 | 16922625 | ID=cds.MS.gene47150.t1;Parent=MS.gene47150.t1 |
| chr6.3 | exon | 16923324 | 16923709 | 16923324 | ID=MS.gene47150.t1.exon2;Parent=MS.gene47150.t1 |
| chr6.3 | CDS | 16923324 | 16923709 | 16923324 | ID=cds.MS.gene47150.t1;Parent=MS.gene47150.t1 |
| Gene Sequence |
| Protein sequence |
